THP
LAPORAN HASIL PENELITIAN PROGRAM RESEARCH GRANT I-MHERE JURUSAN TAHUN ANGGARAN 2010
Judul
Ketua Anggota
: ISOLASI DAN KARAKTERISASI BAKTERI ASAM LAKTAT INDIGENOUS PENGHASIL ANTIBAKTERI DARI FESES BAYI :Dr. Ir. Joni Kusnadi, MSi. :Tunjung Mahatmanto, STP, MSi.
Dibiayai Oleh Direktorat Jenderal Pendidikan Tinggi, Kementerian Pendidikan Nasional, melalui DIPA Universitas Brawijaya berdasarkan SK Rektor Nomor : 214/SK/2010, tanggal 22 Juli 2010.
UNIVERSITAS BRAWIJAYA NOPEMBER 2010
RINGKASAN
Penelitian ini bertujuan untuk mengisolasi, mengidentifikasi dan mengkarakterisasi bakteri asam laktat bersifat anaerob yang menghasilkan senyawa antimikroba dari feces bayi. Metode yang digunakan dalam penelitian ini adalah eksploratif deskriptif. Dari feces bayi yang hanya mengkonsumsi ASI dilakukan isolasi bakteri secara anaerob, dan didapatkan 5 isolat yang mempunyai aktivitas antimikroba pada supernatan bakteri yang pH-nya sudah dinetralkan. Hasil identifikasi dengan API 50 CHL, ke-lima bakteri tersebut menunjukkan kedekatan dengan persentase identitas 99,2% mendekati bakteri Lactobacillus paracasei.
Kata Kunci : Feses bayi, bakteri asam laktat, anaerob, anti mikroba
SUMMARY
The aim of this research was to isolate, identify and to characterize anaerobic lactic acid bacteria isolated from infant feces which have capability to produce antimicrobial compound. Explorative descriptive method was in this experimental work. From infant feces who consumed only ASI, 5 isolates of lactic acid bacteria confering antimicrobial activity were succesfully isolated. Further identification using API 50 C HL test kit showed that all 5 isolates were Lactobacillus paracasei with percentage identity 99,2%.
Key Word : Infant feces, lactic acid bacteria, anaerobic, antimicrobial
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