THP
LAPORAN HASIL PENELITIAN PROGRAM RESEARCH GRANT I-MHERE JURUSAN TAHUN ANGGARAN 2010
Judul
Ketua Anggota
: ISOLASI DAN KARAKTERISASI BAKTERI ASAM LAKTAT INDIGENOUS PENGHASIL ANTIBAKTERI DARI FESES BAYI :Dr. Ir. Joni Kusnadi, MSi. :Tunjung Mahatmanto, STP, MSi.
Dibiayai Oleh Direktorat Jenderal Pendidikan Tinggi, Kementerian Pendidikan Nasional, melalui DIPA Universitas Brawijaya berdasarkan SK Rektor Nomor : 214/SK/2010, tanggal 22 Juli 2010.
UNIVERSITAS BRAWIJAYA NOPEMBER 2010
RINGKASAN
Penelitian ini bertujuan untuk mengisolasi, mengidentifikasi dan mengkarakterisasi bakteri asam laktat bersifat anaerob yang menghasilkan senyawa antimikroba dari feces bayi. Metode yang digunakan dalam penelitian ini adalah eksploratif deskriptif. Dari feces bayi yang hanya mengkonsumsi ASI dilakukan isolasi bakteri secara anaerob, dan didapatkan 5 isolat yang mempunyai aktivitas antimikroba pada supernatan bakteri yang pH-nya sudah dinetralkan. Hasil identifikasi dengan API 50 CHL, ke-lima bakteri tersebut menunjukkan kedekatan dengan persentase identitas 99,2% mendekati bakteri Lactobacillus paracasei.
Kata Kunci : Feses bayi, bakteri asam laktat, anaerob, anti mikroba
SUMMARY
The aim of this research was to isolate, identify and to characterize anaerobic lactic acid bacteria isolated from infant feces which have capability to produce antimicrobial compound. Explorative descriptive method was in this experimental work. From infant feces who consumed only ASI, 5 isolates of lactic acid bacteria confering antimicrobial activity were succesfully isolated. Further identification using API 50 C HL test kit showed that all 5 isolates were Lactobacillus paracasei with percentage identity 99,2%.
Key Word : Infant feces, lactic acid bacteria, anaerobic, antimicrobial
DAFTAR PUSTAKA
Ahl, U. V., Mozzetti, V., Lacroix, C., Kheadr, E. E., Fliss, I. and Meile, L.. 2007. Classification of a moderately oxygen-tolerant isolate from baby faeces as Bifidobacterium thermophilum. BMC Microbiology 2007, 7:79 doi:10.1186/1471-2180-7-79 Abdelbasset, M and Djamila, K., 2008., Antimicrobial activity of autochthonous lactic acid bacteria isolated from Algerian traditional fermented milk “Raïb” African Journal of Biotechnology Vol. 7 (16), pp. 2908-2914, 18 August, 2008 Available online at http://www.academicjournals.org/AJB Anonymous. 2004. Bacteriocin. http://en.wikipedia.org/wiki/Bacteriocin. Tanggal akses 5 September 2005 Anonymous. 2006. Bifidobacterium-From MicrobeWiki, the student-edited microbiology resource. http://microbewiki.kenyon.edu/index.php/ Bifidobacterium. Diakses 18 Agustus 2009 Anonymous. 2008. Lacto Spore-Bacillus http://www.lactospore.com/back.htm. Diakses 22 Juni 2009
coagulans.
Anonymous. 2009a. Lactic Acid Bacteria http://en.wikipedia.org/wik. Diakses 22 Juni 2009 Anonymous. 2009b. Isolasi Bakteri Asam Laktat Penghasil http://iqbalali.com/2008/03/30/isolasi-bakteri-asam-laktatpenghasilantimikroba/. Diakses 22 Juni 2009
Antimikroba.
Anonymous. 2009c. Bifidobacterium. http://en.wikipedia.org/wiki/Bifidobacterium. Diakses 18 Agustus 2009 Barasubramanyam, B.V and M.C. Varadaraj. 1995 Antibacterial effect ofLactobacillus spp. On foodborne pathogenic bacteria in an Indian milk based fermented culinary food item. Culture Dairy Product J 30:22-24, 26-27. Beasley, S. 2004. Isolation, Identification and Exploitation of Lactic Acid Bacteria from Human and Animal Microbiota. Academic Dissertation in Microbiology Bell, C., Neaves, P., and Williams, A.P. 2005. Food Microbiology and Laboratory Practice. Blackwell Publishing. London Chen, Y. S., Yanagida, F., and Srionnual. Characteristics of Bacteriocin-like Inhibitory Substances from Dochi-isolated Enterococcus faecium D081821 and D081833. Journal Compilation 2006 The Society for Applied Microbiology, Letters in Applied Microbiology 44 (2007) 320-325 Cleusix, V., Lacroix, C., Vollenweider, S., Duboux, M. and Le Blay G. 2007 Inhibitory activity spectrum of reuterin produced by Lactobacillus reuteri against intestinal bacteria. BMC Microbiology 2007, 7:101doi:10.1186/1471- 2180-7101 Coolborn, A. F. 2005. Antibacterial Quantification from Lactic Acid Bacteria Isolated from Food Sources and Soil. Journal of Food Technology 3 (4): 568-571, 2005
Dart, R. K. 1996. Microbiology of The Analytical Chemist. The Royal Society of Chemistry. London Dugas, J. 2009. Lactic Acid Bacteria. http://www.waksmanfo. Diakses 22 Juni 2009 Fox, P. F., T. M. Cogan, T. P. Guinee, P. L. H. McSweeney. 2000. Fundamental of Cheese Science. Aspen Publishers, Inc. Maryland Guessas, B. and Kihal, M. 2004. Characterization of Lactic Acid Bacteria Isolated from Algerian Arid Zone Raw Goats’ Milk. African Journal of Biotechnology Vol 3 (6), pp. 339-342, June 2004 Hadadji, M., Benama, R., Saidi, N., Henni, D. E., and Kihal, M. 2005. Identification of Cultivable Bifidobacterium Species Isolated from Breast-fed Infants Feces in West Algeria. African Journal of Biotechnology Vol 4 (4), pp. 422- 430, May 2005 Haddadin, M. S. Y., Awaisheh, S. S., and Robinson, R. K. 2004. The production of Yoghurt with Probiotic Bacteria Isolated from Infants in Jordan. Pakistan Journal of Nutrition 3 (5): 290-293, 2004 Heu, et al. 2001. gly Gene Cloning and Expression and Purification ofGlycinecin A, a Bacteriocin Produced by Xanthomonas campestris pv. glycines 8ra. Applied and Environmental Microbiology, p. 4105-411 Hosono A, Wardojo R, Otani H (1989). Microbial flora in (Dadih), a traditional fermented milk in Indonesia. Lebensm Wiss Tec. 22: 20-24. Khalil, R., Mahrous, H., El-Halafawy, K., Kamaly, K., Frank, J., and El-Soda, M. 2007. Evaluation of the Probiotic Potential of Lactic Acid Bacteria Isolated from Faeces of Breast-fed Infants in Egypt. African Journal of Biotechnology Vol. 6 (7), pp. 939-949, 2 April 2007 Nair, P. S. and Surendran, P. K. 2005. Biochemical Characterization of Lactic Acid Bacteria Isolated from Fish and Prawn. Journal of Culture Collections Volume 4, 2004-2005, pp. 48-52 Ogunbawo, S. T., A. I. Sanni, A. A. Onilude. 2003. Characterization of Bacteriocin Produced by Lactobacillus plantarum F1 and Lactobacillus brevis OG1. African Journal of Biotechnology Vol.2 (8), pp. 219-227. Rodriguez, A. V., and Manca de Nadra, M. C. 1995. Production of hydrogen peroxide by Lactobacillus hilgardii and its effect on Leuconostoc oenos growth. Current Microbiology Volume 30, Number 1 / January, 1995 Salha, H. A. and Fozyah, S. Z., 2006., Production of Bacteriocin(s) by Four Lactic acid Bacteria Isolated from Raw Milk on Organic Waste., world Applied Science Journal 1 (2): 135-143, 2006 Salminen, S., Wright, A.V., and Ouwehand, A. 2004. Lactic Acid Bacteria. Microbiological and Functional Aspects. Marcel Dekker, Inc. New York Savadogo, A., Ouattara, C. A. T., Bassole, I.H. N., and Traore, A. S. 2004. Antimicrobial Activities of Lactic Acid Bacteria Strain Isolated from Burkina Faso Fermented Milk. Pakistan Journal of Nutrition 3 (3): 174-179, 2004
Shafie, H. E., Nagwa I., Hanem A. E., and Yomna A. M. 2008. Purification and Characterization of Bacteriocin Produced by Isolated Strain of Lactococcus lactis., Journal of Applied Sciences Research, 4(11): 1315- 1321, 2008 INSInet Publication Sunaryanto R., Marwoto B, Lisnawati N, Soemiati A., 2006., Karakterisasi dan Uji Antimikrobia Bakteriosin yang dihasilkan Lactobacillus acidophillus., Perhimpunan Mikrobiologi Indonesia cabang Surakarta. Torres, S., Tracanna, M., Amani, S., González, A., Gutiérrez de Ferro, M., Gutiérrez, S. 2008. Determination of the MIC and MBC of Xenophyllum poposum on Lactobacillus casei http://iadr.confex.com/iadr/argentine08/preliminaryprogram/abstract_122508. htm. Diakses 18 Agustus 2009 Toure, R., Kheadr, E., Lacroix, C., Moroni, O.,and Fliss, I. Production of Antibacterial Substances by Bifidobacterial isolates from infant stool active against Listeria monocytogenes. Journal of Applied Microbiology 2003, 95, 1058-1069 Vlkova, E., Rada, V., Bujnakova, D., Kmet, V. 2004. Enumeration, Isolation, and Identification of Bifidobacteria from Infant Feces. Folia Microbiol. 49 (2), 209212 (2004) Wahyudi, D. 2009. REVIEW: Isolasi, Karakterisasi, Purifikasi Dan Aktivitas Antimikrobia dari Bacteriocin yang Diproduksi Oleh Bakteri Asam Laktat Terhadap Pertumbuhan Beberapa Bakteri Patogen dan Pembusuk Makanan. http://aaknasional.ac.id/berita.php?ct=2009-07-01%2012:28:20. diakses 30 Agustus 2009