THP
LAPORAN HASIL PENELITIAN PROGRAM RESEARCH GRANT I-MHERE JURUSAN TAHUN ANGGARAN 2010
Judul Ketua Anggota
: Sintesis Fosfolipid Mengandung Asam Lemak ω-3 dari Fosfolipid Kedelai dan Minyak Kaya Asam Lemak ω -3 dari Hasil Samping Pengalengan Tuna : Mokhamad Nur, STP, MSc. : Dr. Teti Estiasih, STP, MP
Dibiayai Oleh Direktorat Jenderal Pendidikan Tinggi, Kementerian Pendidikan Nasional, melalui DIPA Universitas Brawijaya berdasarkan SK Rektor Nomor : 214/SK/2010, tanggal 22 Juli 2010.
UNIVERSITAS BRAWIJAYA NOPEMBER 2010
RINGKASAN
Fosfolipid merupakan pengemulsi yang bersifat alami. Fosfolipid komersial yang biasa digunakan oleh industri pangan adalah fosfolipid atau lesitin kedelai. Keunggulan fosfolipid dapat lebih ditingkatkan dengan menggabungkan asam lemak ω-3, terutama EPA (eicosapentaenoic acid, C20:5 ω-3) dan DHA docosahexaenoic acid, C22:6 ω-3), pada struktur fosfolipid sehingga diperoleh fosfolipid terstruktur. Fosfolipid kedelai mengandung asam lemak ω-3 merupakan inovasi baru bahan penyedia asam lemak ω-3 sekaligus juga sebagai pengemulsi. Asam lemak ω-3 merupakan asam lemak yang penting bagi kesehatan dengan sumber utama adalah minyak ikan. Minyak ikan hasil samping pengalengan tuna merupakan sumber asam lemak ω-3 yang potensial dengan keunggulan tinggi asam dokosaheksaenoat. Kadar asam lemak ω-3 dalam minyak ikan dapat ditingkatkan dengan menggunakan teknik kristalisasi pelarut suhu rendah. Pada proses sintesis fosfolipid terstruktur diperlukan sumber asil tinggi asam lemak ω-3. Pada penelitian ini, sumber asil yang digunakan adalah minyak kaya asam lemak ω-3 dalam bentuk asam lemak bebas yang diperoleh dari teknik kristalisasi pelarut suhu rendah. Kristalisasi pelarut suhu rendah dilakukan setelah minyak hasil samping pengalengan tuna disaponifikasi dan diekstrak asam lemaknya. Asam lemak jenuh dengan titik leleh tinggi akan mengkristal sedangkan asam lemak ω-3 karena mempunyai titik leleh rendah diharapkan tetap cair sehingga dapat dipisahkan dengan asam lemak jenuh. Strukturisasi fosfolipid dilakukan dengan cara mengganti asam lemak dari fosfolipid kedelai dengan asam lemak ω-3 dari minyak kaya asam lemak ω-3 dari hasil samping pengelangen tuna yang diperoleh dengan teknik kristalisasi pelarut suhu rendah. Sintesisi fosfolipid terstruktur dilakukan secara asidolisis enzimatis dengan menggunakan lipase dari R.miehei. Faktor yang dikaji pada proses sintesis ini adalah konsentrasi enzim dan lama sintesis. Variabel respon yang diteliti adalah tingkat inkorporasi asam lemak ω-3 (EPA+DHA) pada fosfolipid terstruktur dan nilai HLB. Fosfolipid terstruktur mengandung asam lemak ω-3 yang dihasilkan dengan tingkat inkorporasi EPA+DHA tertinggi selanjutnya dikarakterisasi meliputi profil fosfolipid dan tingkat inkorporasi EPA+DHA pada jenis-jenis fosfolipid. Hasil penelitian menunjukkan bahwa teknik kristalisasi pelarut suhu rendah dengan menggunakan asam lemak bebas hasil hidrolisis sebagai sumber minyak dapat meningkatkan kadar EPA+DHA sebanyak 1,37 kali. Tingkat inkorporasi EPA dan DHA pada struktur fosfolipid dipengaruhi oleh konsentrasi enzim dan lama reaksi asidolisis enzimatis. Ada interaksi antara konsentrasi enzim dan lama reaksi. Pada konsentrasi enzim yang rendah, peningkatan lama reaksi setelah tingkat inkoporasi optimum tercapai cenderung menyebabkan penurunan tingkat inkorporasi EPA+DHA. Pada konsentrasi enzim yang tinggi, lama reaksi tampaknya tidak mempengaruhi tingkat inkorporasi. Tingkat inkorporasi DHA lebih tinggi dari EPA sehingga fosfolipid terstruktur yang dihasilkan sangat sesuai digunakan untuk produk pangan yang memerlukan kadar DHA tinggi seperti makanan bayi. Kemampuan emulsifikasi fosfolipid terstruktur yang ditunjukkan oleh nilai EAI dan ESI dipengaruhi oleh tingkat inkorporasi EPA+DHA. Ada preferensi inkorporasi EPA+DHA pada fosfatidiletanolamin dibandingkan jenis fosfolipid yang lain. Kata kunci: fosfolipid, fosfolipid terstruktur, asam lemak ω-3, asidolisis enzimatis
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