Lampiran 1. Bagan Alur Posedur Pembuatan Pakan Diet Tinggi Lemak. Dicampur rata sampai setengah padat

Lampiran 1. Bagan Alur Posedur Pembuatan Pakan Diet Tinggi Lemak

81% Pakan Standar pellet 551

10% Lemak Kambing 1% Kuning Telur Dicampur rata sampai setengah padat Dibentuk berupa silinder dengan ukuran diameter 0,5 cm panjang 1 cm. Dikeringkan

Pakan Diet Tinggi Lemak

Universitas Sumatera Utara

Lampiran 2. Bagan Alur Pengujian terhadap Profil Lipida Serum Darah Tikus

Tikus

Diukur serum darah Diberi perlakuan selama 21 hari

Kadar profil lipida awal

Diukur kadar profil lipida pada hari ke-1, 7, dan 14.

Kadar profil lipida


Lampiran 3. Bagan Alur Pengambilan Serum Darah Tikus

Tikus

Didislokasikan leher

Dibedah diambil darah dari jantung

Darah Tikus

Didiamkan selama 10 menit Disentrifugasi dengan kecepatan 3000 rpm selama 15 menit Diambil cairan bening Serum siap diukur


Lampiran 4. Bagan Alur Pengukuran Kadar Kolesterol Total

Reagen Kolesterol

Diukur larutan blanko

Diukur larutan standar

Serum

Dipipet sebanyak 10 µl Masukkan dalam tabung reaksi yang berisi larutan reagen kolesterol sebanyak 1 ml Dihomogenkan Diinkubasi pada suhu kamar selama 20 menit Diukur dengan spektrofotometer mikrolab 300 pada panjang gelombang 546 nm Dicatat hasilnya

Kadar Kolesterol Total


Lampiran 5. Bagan Alur Pengukuran Kadar Trigliserida

Reagen Trigliserida Diukur larutan blanko

Diukur larutan standar

Serum

Dipipet sebanyak 10 µl Masukkan dalam tabung reaksi yang berisi larutan reagen trigliserida sebanyak 1 ml Dihomogenkan Diinkubasi pada suhu kamar selama 20 menit Diukur dengan spektrofotometer mikrolab 300 pada panjang gelombang 546 nm Dicatat hasilnya

Kadar Trigliserida


Lampiran 6. Bagan Alur Pengukuran Kadar HDL

200 µl larutan standart HDL

Ditambah 500 µl pereaksi kolesterol yang telah diencerkan dengan akuades ( rasio 4 + 1 ) Diinkubasi pada suhu kamar selama 20 menit Disentrifugasi dengan kecepatan 3000 rpm selama 15 menit Larutan supernatan Diukur larutan blanko Diukur larutan standar

Serum Dipipet sebanyak 100 µl Dimasukkan dalam tabung reaksi yang berisi larutan reagen kolesterol HDL sebanyak 1 ml Dihomogenkan Diinkubasi pada suhu kamar selama 20 menit Diukur pada mikrolab 300 dengan Panjang gelombang 546 nm Dicatat hasilnya Kadar HDL


Lampiran 7. Data hasil Pengukuran Kadar lipida Tikus sebelum perlakuan (mg/dl) Lipida

Kolesterol total

Trigliserida

HDL

71

Non diet selama 21 hari 77

diet selama 21 hari 84

79

80

80

77

82

83

174

214

219

171

210

215

169

203

220

34

32

26

41

36

31

41

40

35

Awal


Lampiran 8. Data hasil Pengukuran Kadar Kolesterol Total Tikus setelah perlakuan (mg/dl) Perlakuan ND

D

ND+VCO100

ND+MK100

D+VCO100

D+MK100

D+VCO75+MK25

D+VCO75+MK25

D+VCO50+MK50

Minggu 1 63 68 69 80 81 80 68 69 70 66 62 64 76 78 83 75 78 81 93 89 96 58 59 56 64 65 64

Minggu 2 65 70 66 90 89 88 73 75 68 65 65 67 86 82 81 62 63 62 67 65 67 45 45 46 48 58 55

minggu 3 84 83 84 95 89 92 82 85 90 59 62 61 83 85 84 63 61 61 58 61 64 43 41 46 49 53 51


Lampiran 9. Data hasil Pengukuran Kadar Trigliserida Tikus setelah perlakuan (mg/dl) Perlakuan ND

D

ND+VCO100

ND+MK100

D+VCO100

D+MK100

D+VCO75+MK25

D+VCO75+MK25

D+VCO50+MK50

Minggu 1 120 128 127 190 194 192 99 98 140 68 68 69 154 150 167 140 138 139 93 89 96 69 68 67 84 82 80

Minggu 2 122 124 120 200 208 204 85 96 71 63 65 62 118 120 116 130 128 132 67 65 67 62 62 62 68 67 68

minggu 3 120 135 125 205 203 207 72 80 82 63 65 60 115 108 96 120 117 123 58 61 64 61 62 50 53 53 53


Lampiran 10. Data hasil Pengukuran Kadar HDL Tikus setelah perlakuan (mg/dl) Perlakuan ND

D

ND+VCO100

ND+MK100

D+VCO100

D+MK100

D+VCO75+MK25

D+VCO75+MK25

D+VCO50+MK50

Minggu 1 36 38 37 39 36 39 38 39 40 42 44 46 39 41 41 43 41 44 37 35 42 37 36 35 43 45 44

Minggu 2 32 32 40 43 45 42 45 46 44 41 41 43 42 50 54 33 32 32 49 40 39 30 31 32 33 42 39

minggu 3 40 38 36 46 43 44 65 65 64 38 42 42 54 51 58 30 31 32 45 45 45 29 25 32 35 37 38


Lampiran 11. Analisis Statistik Data Profil lipida Kontrol Awal

1. Uji normalitas dengan menggunakan uji Kolmogorov-Smirnov

One-Sample Kolmogorov-Smirnov Test Kolesterol total Trigliserida N

HDL

9

9

9

Mean

79,2222

199,4444

35,1111

Std. Deviation

3,92994

21,69741

5,06074

,175

,242

,166

Positive

,112

,213

,122

Negative

-,175

-,242

-,166

Kolmogorov-Smirnov Z

,524

,727

,499

Asymp. Sig. (2-tailed)

,946

,666

,965

Normal Parameters

a,b

Most Extreme Differences Absolute

a. Test distribution is Normal. b. Calculated from data.

2. Uji ANOVA dan uji lanjutan Duncan ANOVA Mean Sum of Squares

df

Square

F

Sig.

3,619

,093

124,429

,000

2,835

,136

Kolesterol

Between Groups

67,556

2

33,778

total

Within Groups

56,000

6

9,333

Total

123,556

8

Between Groups

3677,556

2

1838,778

88,667

6

14,778

3766,222

8

Between Groups

99,556

2

49,778

Within Groups

105,333

6

17,556

Total

204,889

8

Trigliserida

Within Groups Total HDL


Lampiran 11 (lanjutan) Kolesterol Total a

Duncan

Subset for alpha = 0.05 perlakuan

N

1

2

awal/normal

3

75,6667

nondiet selama 21 hari

3

79,6667

diet selama 21 hari

3

79,6667 82,3333

Sig.

,160

,326

Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.

Trigliserida a

Duncan


N

1

awal/normal

3

171,3333


3

diet selama 21 hari

3

Sig.

2

3

209,0000 218,0000 1,000

1,000

1,000

HDL a

Duncan


N

1

diet selama 21 hari

3

30,6667


3

36,0000

awal/normal

3

38,6667

Sig.

,065



Lampiran 12. Analisis Statistik Data Kolesterol Total


One-Sample Kolmogorov-Smirnov Test KT

KT

KT

minggu_1

minggu_2

minggu_3

27

27

27

Mean

72,4074

67,1481

69,2222

Std. Deviation

10,63832

13,04343

16,81193

Absolute

,145

,141

,221

Positive

,145

,141

,178

Negative

-,071

-,124

-,221


,754

,731

1,147


,621

,660

,144

N Normal Parameters

a,b

Most Extreme Differences


2. Uji ANOVA dan uji lanjutan Duncan ANOVA Sum of Squares

df

Mean Square

F

Sig.

60,604

,000

84,045

,000

164,205

,000

KT

Between Groups

2837,185

8

354,648

minggu_1

Within Groups

105,333

18

5,852

Total

2942,519

26

KT

Between Groups

4308,074

8

538,509

minggu_2

Within Groups

115,333

18

6,407

Total

4423,407

26

KT

Between Groups

7249,333

8

906,167

minggu_3

Within Groups

99,333

18

5,519

7348,667

26

Total


Lampiran 12 (lanjutan) Kolesterol Total minggu -1 a

Duncan


N

1

2

3

4

5

D+VCO25+MK75

3

57,6667

ND+MK100

3

64,0000

D+VCO50+MK50

3

64,3333

ND

3

66,6667

ND+VCO100

3

D+MK100

3

78,0000

D+VCO100

3

79,0000

D

3

80,3333

D+VCO75+MK25

3

66,6667 69,0000

92,6667

Sig.

1,000

,217

,253

,278

1,000


Kolesterol Total minggu -2 a

Duncan


N

1

D+VCO25+MK75

3

45,3333

D+VCO50+MK50

3

D+MK100

3

62,3333

ND+MK100

3

65,6667

D+VCO75+MK25

3

66,3333

ND

3

67,0000

ND+VCO100

3

D+VCO100

3

D

3

Sig.

2

3

4

5

6

53,6667

72,0000 83,0000 89,0000 1,000

1,000

,051

1,000

1,000

1,000



Lampiran 12 (lanjutan)

Kolesterol Total minggu -3 a

Duncan


N

1

D+VCO25+MK75

3

43,3333

D+VCO50+MK50

3

ND+MK100

3

60,6667

D+VCO75+MK25

3

61,0000

D+MK100

3

61,6667

ND

3

83,6667

D+VCO100

3

84,0000

ND+VCO100

3

85,6667

D

3

Sig.

2

3

4

5

51,0000

92,0000 1,000

1,000

,628

,337

1,000



Lampiran 13. Analisis Statistik Data Trigliserida


One-Sample Kolmogorov-Smirnov Test TG

TG

TG

minggu_1

minggu_2

minggu_3

27

27

27

Mean

115,1481

101,9259

96,7037

Std. Deviation

41,34350

45,37021

47,61126

Absolute

,170

,234

,192

Positive

,170

,234

,192

Negative

-,122

-,189

-,163


,886

1,215

,996


,413

,105

,274

N Normal Parameters

a,b

Most Extreme Differences



df

Mean Square

F

Sig.

69,688

,000

316,321

,000

261,562

,000

TG

Between Groups

43051,407

8

5381,426

minggu_1

Within Groups

1390,000

18

77,222

Total

44441,407

26

TG

Between Groups

53141,852

8

6642,731

minggu_2

Within Groups

378,000

18

21,000

Total

53519,852

26

TG

Between Groups

58434,963

8

7304,370

minggu_3

Within Groups

502,667

18

27,926

58937,630

26

Total


Lampiran 13 (lanjutan) Trigliserida minggu -1 a

Duncan


N

1

2

3

D+VCO25+MK75

3

68,0000

ND+MK100

3

68,3333

D+VCO50+MK50

3

82,0000

D+VCO75+MK25

3

ND+VCO100

3

112,3333

ND

3

125,0000

D+MK100

3

D+VCO100

3

D

3

4

5

6

82,0000 92,6667

125,0000 139,0000 157,0000 192,0000

Sig.

,080

,154

,094

,067

1,000

1,000


Trigliserida minggu -2 a

Duncan


N

1

2

3

4

D+VCO25MK75

3

62,0000

ND+MK100

3

63,3333

D+VCO75+MK25

3

66,3333

D+VCO50+MK50

3

67,6667

ND+VCO100

3

D+VCO100

3

118,0000

ND

3

122,0000

D+MK100

3

D

3

Sig.

5

84,0000

130,0000 204,0000 ,181

1,000

,299

1,000

1,000

Means for groups in homogeneous subsets are displayed. a.

Uses Harmonic Mean Sample Size = 3,000.


Lampiran 13 (lanjutan) Trigliserida minggu -3

Duncana


N

1

D+VCO50+MK50

3

53,0000

D+VCO25MK75

3

57,6667

D+VCO75+MK25

3

61,0000

ND+MK100

3

62,6667

ND+VCO100

3

D+VCO100

3

D+MK100

3

120,0000

ND

3

126,6667

D

3

Sig.

2

3

4

5

78,0000 106,3333

205,0000 ,053

1,000

1,000

,140

1,000



Lampiran 14. Analisis Statistik Data HDL


One-Sample Kolmogorov-Smirnov Test HDL HDL

minggu_

HDL

minggu_1

2

minggu_3

27

27

27

Mean

39,8889

39,7037

42,5926

Std. Deviation

3,25025

6,53284

11,00091

,126

,181

,156

Positive

,126

,181

,156

Negative

-,090

-,124

-,085


,656

,940

,811


,782

,340

,526

N Normal Parameters

a,b

Most Extreme Differences Absolute



df

Mean Square

F

Sig.

8,919

,000

8,511

,000

86,246

,000

HDL

Between Groups

219,333

8

27,417

minggu_1

Within Groups

55,333

18

3,074

Total

274,667

26

HDL

Between Groups

877,630

8

109,704

minggu_2

Within Groups

232,000

18

12,889

Total

1109,630

26

HDL

Between Groups

3066,519

8

383,315

minggu_3

Within Groups

80,000

18

4,444

3146,519

26

Total


Lampiran 14 (lanjutan) HDL minggu -1 a

Duncan


N

1

2

3

D+VCO25+MK75

3

36,0000

ND

3

37,0000

D

3

38,0000

38,0000

D+VCO75+MK25

3

38,0000

38,0000

ND+VCO100

3

39,0000

39,0000

D+VCO100

3

D+MK100

3

ND+MK100

3

44,0000

D+VCO50+MK50

3

44,0000

40,3333

4

40,3333 42,6667

Sig.

,074

,151

42,6667

,120

,390

Means for groups in homogeneous subsets are displayed. Uses Harmonic Mean Sample Size = 3,000.

HDL minggu -2 a

Duncan


N

1

2

3

4

5

D+VCO25MK75

3

31,0000

D+MK100

3

32,3333

32,3333

ND

3

34,6667

34,6667

D+VCO50+MK50

3

ND+MK100

3

41,6667

41,6667

D+VCO75+MK25

3

42,6667

42,6667

42,6667

D

3

43,3333

43,3333

43,3333

ND+VCO100

3

45,0000

45,0000

D+VCO100

3

Sig.

38,0000

38,0000

48,6667 ,252

,083

,111

,310

,075

Means for groups in homogeneous subsets are displayed. a.

Uses Harmonic Mean Sample Size = 3,000.


Lampiran 14 (lanjutan) HDL minggu -3 a

Duncan


N

1

D+VCO25+MK75

3

28,6667

D+MK100

3

31,0000

D+VCO50+MK50

3

36,6667

ND

3

38,0000

ND+MK100

3

D

3

44,3333

D+VCO75+MK25

3

45,0000

D+VCO100

3

ND+VCO100

3

Sig.

2

3

4

5

6

38,0000 40,6667

54,3333 64,6667 ,192

,449

,139

,703

1,000

1,000



Lampiran 15 Analisis statistik rasio kolesterol total dengan HDL

Test of Homogeneity of Variances

minggu1 minggu2 minggu3

Levene Statistic 4.385 3.810 2.724

df1

df2 8 8 8

Sig. .004 .009 .037

18 18 18

ANOVA

minggu1

Between Groups Within Groups Total Between Groups Within Groups Total Between Groups Within Groups Total

minggu2

minggu3

Sum of Squares 1.276 .180 1.456 4.012 .287 4.299 2.993 .113 3.107

df 8 18 26 8 18 26 8 18 26

Mean Square .160 .010

F 15.954

Sig. .000

.501 .016

31.488

.000

.374 .006

59.426

.000

minggu1 Duncan

a

Perlakuan ND+MK100 D+VCO50+MK50 D+VCO75+MK25 D+VCO25+MK75 D D+MK100 ND+VCO100 D+VCO100 ND Sig.

N 3 3 3 3 3 3 3 3 3

1 1.4667 1.4667 1.5667 1.6000

.150

Subset for alpha = .05 2 3

1.6000 1.7667

.056

4

1.7667 1.8000 1.8000 1.9333 .076

2.1667 1.000

Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3.000.


Lampiran 15 (lanjutan) minggu2 Duncan

a

Perlakuan D+VCO100 D+VCO50+MK50 D+VCO25+MK75 D+MK100 D+VCO75+MK25 ND+MK100 D ND+VCO100 ND Sig.

N 3 3 3 3 3 3 3 3 3

Subset for alpha = .05 1 2 1.2333 1.2667 1.3000 1.3333 1.3667 1.6000 1.7333

.259

.212

3

2.1667 2.3333 .123


minggu3 Duncan

a

Perlakuan D D+VCO25+MK75 ND+VCO100 ND+MK100 D+VCO75+MK25 D+VCO50+MK50 D+MK100 D+VCO100 ND Sig.

N 3 3 3 3 3 3 3 3 3

Subset for alpha = .05 1 2 1.2000 1.2000 1.2333 1.2333 1.2333 1.2667 1.3000 1.3000 1.4333 .190

.054

3

2.3000 1.000



Lampiran 16 Gambar Alat

Spektofotometer Microlab 300 ( Vital Scientific )

Sentrigufe ( Swing type model CD-50 SR Tomy Seiko )

Micropipet


Lampiran 17


Lampiran 1. Bagan Alur Posedur Pembuatan Pakan Diet Tinggi Lemak. Dicampur rata sampai setengah padat

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