LAMPIRAN 1. Bagan pembuatan sediaan krim
Fase minyak
Fase air
-
asam stearat setil alkoho gliseril monostearat Vaselin BHT dimasukkan ke dalam cawan penguap - dilebur di atas penangas air pada suhu 70°C
-
Nipagin Natrium edetat propilen glikol trietanolamin aquadest
- dilarutkan dalam air panas yg telah ditakar pada suhu 70°C
Fase minyak + Fase air - digerus konstan di dalam lumpang panas - ditambahkan 3 tetes pewangi Dasar Krim
Sari tomat - ditimbang masing-masing konsentrasi - dimasukkan bahan dasar krim ke dalam lumpang - ditambahkan sari tomat yang telah ditimbang sedikit demi sedikit hingga homogen Krim sari tomat
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Lampiran 2. Hasil identifikasi tumbuhan
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Lampiran 3. Gambar buah tomat, tomat setelah di potong, tomat di juice dan sari tomat yang telah di freeze drying
Buah tomat
Tomat setelah dipotong kecil - kecil
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Lampiran 3. (Lanjutan)
Tomat di juice
Sari tomat yang telah di freeze drying
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Lampiran 4. Gambar alat-alat penelitian
A
B
C Keterangan: A: Juicer yang berisi sampel (Miyako), B: Skin analyzer (Aramo), C: Moisturizer checker (Aramo), D: pH meter (Hanna Instrumen).
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Lampiran 5. Gambar sediaan setelah dimasukkan kedalam wadah dan gambar sediaan setelah penyimpanan hari ke–90 a. Gambar sediaan setelah dimasukkan kedalam wadah
Keterangan : Formula A : Blanko (dasar krim tanpa sampel). Formula B : krim sari tomat 5% Formula C : krim sari tomat 7,5% Formula D : krim sari tomat 10% Formula E : krim sari tomat 12%
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Lampiran 5. (Lanjutan) b. Gambar sediaan setelah penyimpanan hari ke - 90
Keterangan : Formula A : Blanko (dasar krim tanpa sampel). Formula B : krim sari tomat 5% Formula C : krim sari tomat 7,5% Formula D : krim sari tomat 10% Formula E : krim sari tomat 12%
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Lampiran 6. Gambar hasil uji homogenitas dan tipe emulsi a. Uji homogenitas
FA
FB
FC
FD
FE
FF
b. Tipe emulsi
FA
FB
FC
FD
FE
FF
Keterangan : Formula A : Blanko (dasar krim tanpa sampel). Formula B : krim sari tomat 5% Formula C : krim sari tomat 7,5% Formula D : krim sari tomat 10% Formula E : krim sari tomat 12%
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Lampiran 7. Salah satu contoh hasil uji efektivitas anti-aging pada sukarelawan a.. Hasil pengukuran kadar air (Moisture) - Kondisi awal
- Pemulihan minggu pertama (Minggu1)
- Pemulihan minggu kedua (Minggu 2)
- Pemulihan minggu ketiga (Minggu 3)
- Pemulihan minggu ke empat (Minggu 4)
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Lampiran 7. (Lanjutan) a. Hasil pengukuran kehalusan dan besar pori (Evenness/pore) - Kondisi awal
- Pemulihan minggu pertama (Minggu1)
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Lampiran 7. (Lanjutan) - Pemulihan minggu kedua (Minggu 2)
- Pemulihan minggu ketiga (Minggu 3)
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Lampiran 7. (Lanjutan) - Pemulihan minggu ke empat (Minggu 4)
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Lampiran 7. (Lanjutan) b. Hasil pengukuran banyak noda (spot) - Kondisi awal
- Pemulihan minggu pertama (Minggu1)
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Lampiran 7. (Lanjutan) - Pemulihan minggu pertama (Minggu2)
- Pemulihan minggu pertama (Minggu3)
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Lampiran 7. (Lanjutan) - Pemulihan minggu pertama (Minggu4)
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Lampiran 8. Hasil variansi (ANAVA) dan Tukey untuk sukarelawan selama 4 minggu
pemulihan kulit
a. Kadar air (Moisture) ANOVA Sum of Squares minggu0
minggu1
minggu2
minggu3
minggu4
Between Groups
df
Mean Square
2.000
5
.400
Within Groups
12.000
12
1.000
Total
14.000
17
Between Groups
12.944
5
2.589
Within Groups
18.667
12
1.556
Total
31.611
17
Between Groups
42.944
5
8.589
Within Groups
12.667
12
1.056
Total
55.611
17
Between Groups
57.778
5
11.556
Within Groups
10.000
12
.833
Total
67.778
17
125.611
5
25.122
11.333
12
.944
136.944
17
Between Groups Within Groups Total
F
Sig. .400
.840
1.664
.217
8.137
.001
13.867
.000
26.600
.000
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minggu0 TukeyHSDa Subset for alpha = 0.05 Formula
N
1
Blanko
3
28.00
KST 5%
3
27.67
KST 7,5%
3
28.00
KST 10%
3
27.67
KST 12%
3
28.67
Olay
3
28.00
Sig.
.817
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
minggu1 TukeyHSDa Subset for alpha = 0.05 Formula
N
1
Blanko
3
28.00
KST 5%
3
28.33
KST 7,5%
3
29.33
KST 10%
3
29.67
KST 12%
3
30.00
OLAY
3
30.33
Sig.
.269
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
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minggu2 TukeyHSDa Subset for alpha = 0.05 Formula
N
1
2
3
Blanko
3
28.00
KST 5%
3
29.33
KST 7,5%
3
31.33
31.33
KST 10%
3
31.33
31.33
KST 12%
3
32.00
32.00
OLAY
3
29.33
32.33
Sig.
.620
.067
.833
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
minggu3 TukeyHSDa Subset for alpha = 0.05 Formula
N
1
2
3
Blanko
3
29.00
KST 5%
3
30.00
KST 7,5%
3
KST 10%
3
32.67
KST 12%
3
33.33
OLAY
3
34.00
Sig.
30.00 32.33
.758
.073
32.33
.290
Means for groups in homogeneous subsets are displayed. a.
Uses Harmonic Mean Sample Size = 3,000.
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minggu4 TukeyHSDa Subset for alpha = 0.05 Formula
N
1
2
3
Blanko
3
29.33
KST 5%
3
30.00
KST 7,5%
3
33.33
KST 10%
3
33.67
KST 12%
3
35.33
OLAY
3
Sig.
35.33 36.67
.954
.192
.567
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
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Lampiran 8. (Lanjutan) b. Kehalusan (Evenness) ANOVA Sum of Squares minggu0
minggu2
minggu3
minggu4
Mean Square
Between Groups
4.667
5
.933
Within Groups
9.333
12
.778
14.000
17
9.333
5
1.867
Within Groups
12.667
12
1.056
Total
22.000
17
Between Groups
37.111
5
7.422
Within Groups
10.000
12
.833
Total
47.111
17
Between Groups
78.278
5
15.656
Within Groups
18.000
12
1.500
Total
96.278
17
Between Groups
98.278
5
19.656
8.667
12
.722
106.944
17
Total minggu1
df
Between Groups
Within Groups Total
F
Sig.
1.200
.366
1.768
.194
8.907
.001
10.437
.000
27.215
.000
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minggu0 TukeyHSDa Subset for alpha = 0.05 Formula
N
1
Olay
3
35.67
KST 12%
3
34.67
KST 10%
3
35.67
KST 7,5%
3
35.67
KST 5%
3
36.33
Blanko
3
36.00
Sig.
.260
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
minggu1 TukeyHSDa Subset for alpha = 0.05 Formula
N
1
OLAY
3
34.00
KST 12%
3
34.33
KST 10%
3
34.67
KST 7,5%
3
35.33
Blanko
3
35.67
KST 5%
3
36.00
Sig.
.235
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
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minggu2 TukeyHSDa Subset for alpha = 0.05 Formula
N
1
2
3
OLAY
3
32.00
KST 12%
3
32.33
32.33
KST 10%
3
33.00
33.00
KST 5%
3
34.67
34.67
KST 7,5%
3
34.67
34.67
Blanko
3
36.00
Sig.
.758
.073
.506
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
minggu3 TukeyHSDa Subset for alpha = 0.05 Formula
N
1
2
KST 12%
3
30.00
KST 10%
3
31.00
OLAY
3
31.00
KST 7,5%
3
33.33
KST 5%
3
34.67
Blanko
3
35.67
Sig.
33.33
.052
.253
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
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minggu4
TukeyHSDa Subset for alpha = 0.05 Formula
N
1
2
3
OLAY
3
28.00
KST 12%
3
29.00
29.00
KST 10%
3
30.00
30.00
KST 7,5%
3
KST 5%
3
Blanko
3
Sig.
31.00
4
31.00 32.67 35.00
.110
.110
.229
1.000
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
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Lampiran 8. (Lanjutan) c. pori (pore) ANOVA Sum of Squares minggu0
minggu1
47.611
5
9.522
Within Groups
62.000
12
5.167
Total
109.611
17
Between Groups
104.500
5
20.900
78.000
12
6.500
Total
182.500
17
Between Groups
277.111
5
55.422
72.667
12
6.056
Total
349.778
17
Between Groups
445.778
5
89.156
70.000
12
5.833
Total
515.778
17
Between Groups
625.833
5
125.167
96.667
12
8.056
722.500
17
Within Groups
minggu3
Within Groups
minggu4
Mean Square
Between Groups
Within Groups
minggu2
df
Within Groups Total
F
Sig.
1.843
.179
3.215
.045
9.152
.001
15.284
.000
15.538
.000
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minggu0 Tukey HSDa Subset for alpha = 0.05 Formula
N
1
Olay
3
31.33
KST 12%
3
32.33
KST 10%
3
32.33
KST 7,5%
3
32.67
KST 5%
3
32.33
Blanko
3
35.33
Sig.
.253
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
minggu1 Tukey HSDa Subset for alpha = 0.05 Formula
N
1
2
OLAY
3
28.33
KST 12%
3
32.00
32.00
KST 10%
3
31.00
31.00
KST 7,5%
3
31.33
31.33
KST 5%
3
35.00
35.00
Blanko
3
Sig.
35.33 .065
.357
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
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minggu2 Tukey HSDa Subset foralpha = 0.05 Formula
N
1
OLAY
3
23.00
KST 12%
3
28.67
28.67
KST 10%
3
29.00
29.00
KST 7,5%
3
29.67
29.67
KST 5%
3
35.33
Blanko
3
35.33
Sig.
.054
.093
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
minggu3 Tukey HSDa Subset for alpha = 0.05 Formula
N
1
2
3
4
OLAY
3
KST 12%
3
25.00
KST 10%
3
26.67
26.67
KST 7,5%
3
27.00
27.00
KST 5%
3
Blanko
3
Sig.
18.33
32.00
32.00 33.67
1.000
.904
.145
.953
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
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minggu4 Tukey HSDa Subset for alpha = 0.05 Formula
N
1
2
3
4
OLAY
3
15.67
KST 12%
3
22.33
KST 10%
3
24.00
24.00
KST 7,5%
3
26.00
26.00
26.00
KST 5%
3
31.67
31.67
Blanko
3
Sig.
22.33
33.33 .111
.624
.054
.069
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
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Lampiran 8. (Lanjutan) d. Noda (spot) ANOVA Sum of Squares minggu0
minggu1
minggu2
minggu3
Mean Square
Between Groups
10.500
5
2.100
Within Groups
28.000
12
2.333
Total
38.500
17
Between Groups
37.833
5
7.567
Within Groups
34.667
12
2.889
Total
72.500
17
Between Groups
81.333
5
16.267
Within Groups
32.667
12
2.722
Total
114.000
17
Between Groups
203.111
5
40.622
38.667
12
3.222
Total
241.778
17
Between Groups
379.167
5
75.833
55.333
12
4.611
434.500
17
Within Groups
minggu4
df
Within Groups Total
F
Sig. .900
.512
2.619
.080
5.976
.005
12.607
.000
16.446
.000
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minggu0 TukeyHSDa Subset for alpha = 0.05 Formula
N
1
OLAY
3
29.67
KST 7,5%
3
31.00
KST 10%
3
31.00
KST 5%
3
31.67
KST 12%
3
31.67
Blanko
3
32.00
Sig.
.462
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
minggu1 TukeyHSDa Subset for alpha = 0.05 Formula
N
1
OLAY
3
28.33
KST 12%
3
28.33
KST 10%
3
30.00
KST 7,5%
3
30.67
KST 5%
3
31.67
Blanko
3
32.00
Sig.
.160
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
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minggu2 TukeyHSDa Subset for alpha = 0.05 Formula
N
1
2
KST 12%
3
26.33
OLAY
3
26.67
KST 10%
3
28.67
28.67
KST 7,5%
3
29.00
29.00
KST 5%
3
31.33
Blanko
3
32.00
Sig.
.406
.206
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
minggu3 TukeyHSDa Subset for alpha = 0.05 Formula
N
1
2
3
4
OLAY
3
22.33
KST 12%
3
24.33
24.33
KST 10%
3
25.67
25.67
25.67
KST 7,5%
3
28.00
28.00
28.00
KST 5%
3
30.33
30.33
Blanko
3
Sig.
32.00 .275
.198
.067
.140
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
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minggu4 TukeyHSDa Subset for alpha = 0.05 Formula
N
1
2
3
OLAY
3
19.33
KST 12%
3
21.33
KST 10%
3
23.00
KST 7,5%
3
KST 5%
3
30.33
Blanko
3
31.67
Sig.
23.00 27.33
.352
.207
27.33
.207
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
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SURAT PERNYATAAN PERSETUJUAN IKUT SERTA DALAM PENELITIAN
Nama lengkap
:……………………………………………..
Usia
:…………………………………………….
Jenis kelamin
:…………………………………………….
Alamat
:…………………………………………….
Bersedia menjadi sukarelawan untuk test anti-aging ?…………. Telah mendapat penjelasan secukupnya bahwa tangan saya akan digunakan sebagai daerah yang akan dianalisis. Setelah mendapat penjelasan secukupnya tentang manfaat penelitian ini maka saya menyatakan SETUJU untuk ikut serta dalam penelitian DARA YUNITA dengan judul “FORMULASI SEDIAAN KRIM SARI TOMAT (Solanum lycopersicum L.) DAN UJI EFEK ANTI-AGING ” sebagai usaha untuk mengetahui apakah sediaan krim yang dihasilkan mampu memberikan efek anti penuaan dini. Saya menyatakan sukarela dan bersedia untuk mengikuti prosedur penelitian yang telah ditetapkan. Persetujuan ini saya buat dengan penuh kesadaran dan tanpa paksaan dari pihak manapun. Demikian surat pernyataan ini dibuat untuk dapat dipergunakan sebagaimana mestinya.
Peneliti ,
Medan,
September 2015
Dara Yunita NIM 101501008
NIM
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