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dengan pengencer dasar tris untuk pembekuan semen kambing memiliki hasil yang mirip dengan gliserol. Dengan demikian DMF dapat digunakan sebagai krioprotektan alternatif untuk pembekuan semen kambing.
SIMPULAN DAN SARAN Simpulan Pengencer tris-soya dapat digunakan sebagai pengencer semen cair kambing dan suplementasi rafinosa mempertahankan daya hidup spermatozoa dalam tris-soya sampai 60 jam, sedangkan suplementasi trehalosa mempertahankan daya hidup spermatozoa dalam tris-kuning telur sampai 84 jam. Pengencer tris-kuning telur trehalosa dengan penambahan gliserol menunjukkan peranan yang lebih baik dalam melindungi kualitas spermatozoa pada saat pembekuan dibandingkan dengan pengencer tris-soya rafinosa dengan penambahan DMF. Pemeriksaan motilitas spermatozoa dapat menggambarkan pemeriksaan membran plasma utuh dan spermatozoa hidup. Saran Penelitian ini merupakan penelitian pertama untuk mengevaluasi efek dari lesitin kacang kedelai untuk preservasi dan kriopreservasi semen kambing PE, sehingga diperlukan penelitian lebih lanjut untuk mendapatkan informasi tentang lesitin kacang kedelai. Untuk membuat semen cair kambing dapat digunakan pengencer tris-kuning telur dengan suplementasi trehalosa 50 mM dan tris-soya dengan suplementasi rafinosa 50 mM. Perlu dilakukan IB pada kambing betina menggunakan semen cair hasil penelitian ini untuk mengetahui fertilitas spermatozoa yang sebenarnya.
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