LAMPIRAN A. Hasil Uji Mutu Fisik Granul

LAMPIRAN A

Hasil Uji Mutu Fisik Granul Mutu fisik yang diuji

Kadar air (persen)

Batch I II III X SD I

Waktu alir (detik)

II

III

Di uji 1 2 3

1 2 3 1 2 3 1 2 3

X SD I

Sudut diam (derajat)

II

III

1 2 3 1 2 3 1 2 3

X SD I

Indeks Kompresibilitas (persen)

II

III X SD

1 2 3 1 2 3 1 2 3

Formula Tablet Salbutamol FA FB FC FD 4,67 4,75 4,39 4,3 4,65 4,73 4,37 4,28 4,69 4,77 4,41 4,32 4,67 475 4,39 4,3 0,02 0,02 0,02 0,02 9,69 9,55 9,39 9,11 9,65 9,53 9,33 9,12 9,67 9,52 9,37 9,15 9,64 9,55 9,38 9,17 9,66 9,58 9,35 9,18 9,63 9,57 9,34 9,11 9,69 9,54 9,38 9,15 9,68 9,55 9,36 9,14 9,67 9,59 9,37 9,13 9,66 9,55 9,36 9,14 0,02 0,02 0,01 0,02 35,62 36,1 36,6 35,33 34,69 35,24 36,98 35,95 35,3 36,18 35,8 35,12 34,38 35,19 35,9 36,91 34,76 36,12 36,87 37,1 35,76 35,87 36,2 35,76 35,9 35,9 36,98 36,99 34,1 36,58 35,17 35,13 34,96 36,97 35,67 36,96 35,05 36,01 36,15 36,13 0,63 0,56 0,73 0,85 13,48 13,78 14,01 13,94 13,25 13,51 14,62 13,35 13,48 13,51 14,01 13,35 13,91 13,78 14,62 13,94 13,48 13,32 13,5 14,05 13,69 13,32 13,5 14,05 13,25 13,78 14,79 13,9 13,48 13,51 13,89 13,35 13,69 13,78 13,89 13,35 13,52 13,58 14,09 13,69 0,21 0,19 0,47 0,33

67

Persyaratan

3-5 (Voigt, 1995)

Tidak lebih dari 10 detik (Banker & Anderson, 1986)

25-40 (Banker & Anderson, 1986)

11-15 = baik

(Siregar, 1992)

LAMPIRAN B

Hasil Uji Kekerasan Tablet Salbutamol Batch I Kekerasan Salbutamol Formula A Formula B 13,6 13,4 1 13,7 14,1 2 13,3 13,8 3 13,8 13,4 4 13,9 13,7 5 13,6 14,2 6 13,4 13,9 7 13,9 14,1 8 13,8 13,3 9 13,7 13,7 10 X±SD 13,67±0,20 13,76±0,32

Tablet

No

Formula C Formula D 14,0 13,9 14,1 14,1 14,2 13,9 14,0 14,2 13,9 13,8 13,8 13,9 13,9 14,1 13,9 14,3 14,1 14,0 14,0 13,9 14,01±0,15 13,99±0,11

Batch II No 1 2 3 4 5 6 7 8 9 10 X±SD

Formula A 13,5 13,6 13,1 14,1 14,0 13,9 13,2 13,9 13,1 13,9 13,63±0,38

Kekerasan Tablet Salbutamol Formula B Formula C 13,5 13,7 13,6 13,8 13,1 14,0 14,1 14,1 14,0 14,1 13,9 13,9 13,2 13,8 12,9 14,1 13,1 13,7 12,5 13,9 13,39±0,51 13,91±0,15

68

Formula D 14,1 13,7 14,1 14,2 13,8 13,8 14,0 13,9 14,1 14,2 13,99±0,17

Batch III Kekerasan Tablet Salbutamol Formula A Formula B Formula C Formula D 1 13,7 13,2 13,5 13,9 2 13,5 13,8 13,7 13,8 3 13,4 14,1 13,9 14,1 4 13,7 13,7 14,0 14,2 5 13,1 13,8 14,1 14,1 6 13,9 13,2 14,1 13,9 7 13,7 13,2 13,9 13,9 8 13,0 13,6 13,8 14,1 9 13,3 14,1 14,1 14,2 10 13,8 14,0 14,0 14,0 X±SD 13,51±0,30 13,67±0,36 13,91±0,19 14,02±0,13 No

69

LAMPIRAN C

Hasil Uji Kerapuhan Tablet Salbutamol Batch I

Formula Replikasi 1 2 3 A 1 2 3 B 1 2 3 C 1 2 3 D

Berat awal (gram) 5,989 5,997 5,983 5,813 5,815 5,802 5,997 5,951 6,031 6,023 5,997 5,938

Berat akhir (gram) 5,978 5,986 5,972 5,802 5,804 5,791 5,986 5,940 6,020 6,012 5,986 5,927

Kerapuhan (%) 0,184 0,183 0,184 0,189 0,189 0,190 0,183 0,185 0,182 0,183 0,183 0,185

X±SD 0,183±0,000215

0,189±0,000228

0,183±0,001231

0,184±0,001341

Batch II

Formula

A

B

C

D

Berat awal Replikasi (gram) 1 5,989 2 5,997 3 5,983 1 5,813 2 5,815 3 5,802 1 5,997 2 5,951 3 6,031 1 6,023 2 5,997 3 5,938

Berat akhir (gram) 5,978 5,986 5,972 5,802 5,804 5,791 5,986 5,940 6,020 6,012 5,986 5,927 70

Kerapuhan (%) 0,184 0,183 0,184 0,189 0,189 0,190 0,183 0,185 0,182 0,183 0,183 0,185

X±SD 0,183±0,000215

0,189±0,000228

0.183±0,001231

0,184±0,001341

Batch III

Formula

A

B

C D

Replikasi 1 2 3 1 2 3 1 2 3 1 2

Berat awal (gram) 5,989 5,997 5,983 5,813 5,815 5,802 5,997 5,951 6,031 6,023 5,997

Berat akhir (gram) 5,978 5,986 5,972 5,802 5,804 5,791 5,986 5,940 6,020 6,012 5,986

71

Kerapuhan (%) 0,184 0,183 0,184 0,189 0,189 0,190 0,183 0,185 0,182 0,183 0,183

X±SD 0,183±0,000215

0,189±0,000228

0.183±0,001231

0,184±0,001341

LAMPIRAN D

Hasil Penetapan Kadar Tablet Lepas Lambat Salbutamol Batch I Formula A

B

C

D

Replikasi 1 2 3 1 2 3 1 2 3 1 2 3

absorbansi 0,045 0,044 0,044 0,043 0,044 0,045 0,044 0,044 0,045 0,044 0,046 0,043

Csampel 6,3056 6,1667 6,2222 6,0278 6,1667 6,3611 6,1667 6,1667 6,3611 6,1667 6,4444 6,0833

Cteoritis 6,2587 6,2442 6,2379 6,1506 6,2379 6,4355 6,2587 6,2483 6,4210 6,1526 6,4022 6,1714

%kadar 100,75 98,76 99,75 98,00 98,86 98,84 98,53 98,69 99,07 100,23 100,66 98,57

X±SD 99,75 ± 0,99 98,57 ± 0,48 98,76 ± 0,27 99,82 ± 1,1

Batch II Formula A

B

C

D

Replikasi

absorbansi

Csampel

Cteoritis

%kadar

X±SD

1

0,045

6,3056

6,2566

100,78

100,50

2

0,045

6,3056

6,2421

101,02

±

3

0,044

6,2222

6,2400

99,72

0,69

1

0,043

6,0278

6,1006

98,81

98,87

2

0,044

6,1667

6,2421

98,79

±

3

0,043

6,0833

6,1443

99,01

0,12

1

0,045

6,3056

6,2670

100,61

99,60

2

0,043

6,0278

6,0778

99,18

±

3

0,044

6,2222

6,2837

99,02

0,87

1

0,045

6,3056

6,2483

100,92

100,60

2

0,045

6,3056

6,2774

100,45

±

3

0,044

6,2222

6,1942

100,45

0,26

72

Batch III Formula A

B

C

D

Replikasi

absorbansi

1 2 3 1 2 3 1 2 3 1 2 3

0,044 0,045 0,046 0,045 0,043 0,046 0,043 0,045 0,044 0,044 0,043 0,045

Csampel

Cteoritis

%kadar

X±SD

6,1667 6,3056 6,5000 6,3056 6,0278 6,5000 6,0278 6,3056 6,2222 6,1667 6,0278 6,3611

6,2171 6,2421 6,4376 6,2629 6,0798 6,4397 6,0986 6,2504 6,2795 6,2587 6,0798 6,3086

99,19 101,02 100,97 100,68 99,14 100,94 98,84 100,88 99,09 98,53 99,14 100,83

100,39 ± 1,04 100,25 ± 0,96 99,6 ± 1,11 99,5 ± 1,19

73

LAMPIRAN E

Contoh Perhitungan

Contoh perhitungan sudut diam: Formula A: W persegi panjang

= 4,28 gram

W lingkaran

= 0,88 gram

Luas persegi panjang

= 21,5 x 28 = 602 cm2

Luas lingkaran A

= π.r2

r2

=

=

=

0,88 × 602 = 123,78 cm2 4,28

A π

123,78 = 39,42 3,14

r

= 6,28 cm

tg α

=

α

= 35,62°

4,5 t = = 0,72 r 6,28

Contoh perhitungan indeks kompresibilitas: Formula A : Berat gelas = 125,89 g (W1) Berat gelas + granul = 165,69 g (W2) V1 = 100 ml 74

V2 = 86 ml

(W2 − W1 ) (165,69 − 125,89) = = 0,398 100 V1

Bj nyata =

(W2 − W1 ) (165,69 − 125,89) = = 0,46 87 V2

Bj mampat

=

% kompresibilitas

= 1 − 

 

Bj.nyata   x 100 % = 13,48% Bj.mampat 

Contoh perhitungan akurasi & presisi: Bahan %

aktif (mg)

100

9,6

Matriks (mg)

290,4

+Dapar

+Dapar fosfat pH 6,8

Pipet

ad

fosfat pH 6,8 ad

100

0,65

10

Konsent

rasi (ppm) 6,24

Absorbansi = 0,044 → y = 0,0072x – 0,0004 Konsentrasi sebenarnya = 6,1667 ppm Konsentrasi teoritis = 6,2338 ppm % perolehan kembali = (konsentrasi sebenarnya / konsentrasi teoritis) x 100% = (6,1667 / 6,2338) x 100% = 98,92 % Untuk menghitung % KV

=

SD x100% X

= 0,93 x100 % 99,72 = 0,9331 %

75

Contoh perhitungan % obat terlepas: % obat terlepas =

Wt PK × dosis 100

× 100%

Formula A replikasi 1 pada t = 30 menit % obat terlepas =

2,4072 × 100% = 25,02% 100,22 × 9,6 100

Contoh perhitungan AUC pada disolusi: Rumus: Wtn + W tn-1 x tn – tn-1 2 Formula A batch 1 Wtn-1 = 2,4072 Wtn

= 3,1779

tn = 90 menit tn-1 = 60 menit AUC = 2,4072 + 3,1779 x(90 − 60) 2 =83,7765

Luas □ = 360 x penetapan kadar x dosis = 360 x 100,22% x 9,6 mg = 3463,6032

% ED Formula A batch 1= (∑ AUC / luas □) x 100% = (2268.7611/3463,6032) x 100% = 65,50 %

76

Perhitungan persamaan orde satu: Rumus: ln ( X -Ct) = ln C0 - k . t Dari persamaan regresi ln ( X -Ct) versus t (waktu), maka didapatkan suatu persamaan regresi dan nilai r, slope serta intersept. Nilai kdiss adalah –slope.

X adalah rata-rata penetapan kadar.

77

LAMPIRAN F

Persamaan Formula A Persamaan Orde Nol

15 10 5 0

Formula A

0

200

Linear (Formula A)

400 r = 0.980345 r tabel = 0,404

Persamaan Higuchi

20 Formula A

10 0 0

5

10

15

20

Linear (Formula A)

r = 0,9926 r tabel = 0,404 Persamaan orde satu

4 Formula A 2 0 -2 0

200

400

r = -0,9793 r tabel = 0,404 78

Linear (Formula A)

LAMPIRAN G Persamaan Formula B Persamaan orde nol

10 Formula B

5

Linear (Formula B)

0 0

100

200

300

400

r = 0,9859 r tabel = 0,404 Persamaan Higuchi

10 Formula B 5 Linear (Formula B)

0 0

5

10

15

20

r = 0,9988 r tabel = 0,404 Persamaan orde satu

4

Formula B

2 0 -2 0

100

200

300

r = -0,9909 r tabel = 0,404 79

400

Linear (Formula B)

LAMPIRAN H Persamaan Formula C Persamaan Orde Nol

10 Formula C

5 0 0

100

200

300

400

Linear (Formula C)


10 Formula C

5

Linear (Formula C)

0 0

5

10

15

20

r = 0,9960 r tabel = 0,404 Persamaan Orde Satu

4 Formula C 2 0 -2 0

100

200

300

400

r = 0,9890 r tabel = 0,404 80

Linear (Formula C)

LAMPIRAN I Persamaan Formula D Persamaan Orde Nol

10

Formula D

5 0 0

100

200

300

Linear (Formula D)

400


8 6 4 2 0

Formula D Linear (Formula D) 0

5

10

15

20

r = 0,9888 r tabel = 0,404 Persamaan Orde Satu

2 Formula D 1 0 -1 0

100

200

300

400

r = 0,9547 r tabel = 0,404 81

Linear (Formula D)

LAMPIRAN J

Sertifikat Hidroksi Propil Metil Selulosa

82

LAMPIRAN K

Sertifikat Analisis Polivinil Pirolidon K-30

83

LAMPIRAN L

Sertifikat Analisis Talkum

84

LAMPIRAN M

Sertifikat Analisis Magnesium Stearat

85

LAMPIRAN N

Sertifikat Analisis Laktosa

86

LAMPIRAN O Sertifikat Analisis Natrium Hidroksida

87

LAMPIRAN P Sertifikat Analisa Kalium Dihidrogen Fosfat

88

LAMPIRAN Q

Tabel Uji r

89

LAMPIRAN R

Tabel Uji HSD (0,05)

90

LAMPIRAN S

Hasil Uji Statistik Kadar Air Antar Formula Anova: Single Factor SUMMARY Groups Column 1 Column 2 Column 3 Column 4

ANOVA Source of Variation Between Groups Within Groups Total

Count 3 3 3 3

SS

Sum 14.01 14.25 13.17 12.9

Average 4.67 4.75 4.39 4.3

Variance 0.0004 0.0004 0.0004 0.0004

df

MS

F

0.421425

3

0.140475

0.0032

8

0.0004

0.424625

11

3.1875

P-value

F crit

0.1191

4.0661

PENGUJIAN HIPOTESA : a. H : Pi = 0 Yang berarti tidak ada perbedaan EFEK yang signifikan sebagai akibat perbedaan perlakuan. b. KESIMPULAN: Karena F hitung < F(0.05) maka H diterima. Dengan perkataan lain perlakuan-perlakuan tidak memberikan perbedaan efek yang signifikan.

91

LAMPIRAN T

Hasil Uji Statistik Waktu AlirAntar Formula Anova: Single Factor SUMMARY Groups Column 1 Column 2 Column 3 Column 4

Count 3 3 3 3

Sum 29.01 28.6 28.09 27.38

Average 9.67 9.5333 9.3633 9.1266

Variance 0.0004 0.000233 0.000933 0.000433

df

MS

F

Pvalue

F crit

3.91111

0.0683

4.0661

ANOVA Source of Variation Between Groups Within Groups

0.493667 0.004

3 8

Total

0.497667

11

SS

0.1645 0.0005


92

LAMPIRAN U

Hasil Uji Indeks Kompresibilitas Antar Formula Anova: Single Factor SUMMARY Groups Column 1 Column 2 Column 3 Column 4


Count 3 3 3 3

SS

Sum 105.61 107.52 109.38 106.4

Average 35.203 35.84 36.46 35.466

Variance 0.223233 0.2716 0.3628 0.186233

df

MS

F

Pvalue

F crit

3.414602

0.0732

4.0661

2.673292

3

0.8910

2.087733

8

0.2609

4.761025

11


93

LAMPIRAN V Hasil Uji Statistik Sudut Diam Antar Formula Anova: Single Factor SUMMARY Groups Column 1 Column 2 Column 3 Column 4

Count 3 3 3 3

Sum 40.21 40.8 42.64 40.64

Average 13.40333 13.6 14.21333 13.54667

Variance 0.017633 0.0243 0.124033 0.116033

df

MS 0.384697 0.0705

F 3.456698


SS 1.154092 0.564

Total

1.718092

3 8

Pvalue 0.0695

11


94

F crit 4.0661

LAMPIRAN W

Hasil Uji Statistik Kekerasan Tablet Formula A Antar Batch Anova: Single Factor SUMMARY Groups Column 1 Column 2 Column 3

Count 10 10

Sum 136.7 136.3

Average 13.67 13.63

Variance 0.040111 0.149

10

135.1

13.51

0.092111

MS 0.069333 0.093741

F 0.739629


SS 0.138667 2.531

Total

2.669667

df 2 27

Pvalue 0.4867

F crit 3.3541

29


95

LAMPIRAN X

Hasil Uji Statistik Kekerasan Tablet Formula B Antar Batch Anova: Single Factor SUMMARY Groups Column 1 Column 2 Column 3

Count 10 10 10


SS 0.744667 4.534

Total

5.278667

Sum 137.6 133.9 136.7

Average 13.76 13.39 13.67

Variance 0.102667 0.269889 0.131222

df

MS 0.372333 0.167926

F 2.217247

2 27

Pvalue 0.1283

F crit 3.3541

29


96

LAMPIRAN Y

Hasil Uji Statistik Kekerasan Tablet Formula C Antar Batch Anova: Single Factor SUMMARY Groups Column 1 Column 2 Column 3

Count 10 10 10


SS 0.0666667 0.807

Total

0.8736667

Sum 140.1 139.1 139.1

Average 14.01 13.91 13.91

Variance 0.025444 0.025444 0.038778

df

MS 0.033333 0.029889

F 1.115242

2 27

Pvalue 0.3424

29


97

F crit 3.3541

LAMPIRAN Z

Hasil Uji Statistik Kekerasan Tablet Formula D Antar Batch Anova: Single Factor SUMMARY Groups Column 1 Column 2 Column 3

Count 10 10 10

Sum 139.9 139.9 140.2


SS 0.006 0.594

df

Total

0.6

Average 13.99 13.99 14.02

Variance 0.014333 0.032111 0.019556

MS 0.003 0.022

F 0.136364

2 27

Pvalue 0.8731

F crit 3.3541

29


98

LAMPIRAN AA

Hasil Uji Statistik Kekerasan Tablet Antar Formula Batch 1

Anova: Single Factor SUMMARY Groups Column 1 Column 2 Column 3 Column 4


Count 10 10 10 10

Sum 136.7 137.6 140.1 139.9

Average 13.67 13.76 14.01 13.99

Variance 0.040111 0.102667 0.025444 0.014333

SS

df

MS

F

Pvalue

F crit

2.242848

0.0715

2.8662

0.85475

3

0.284917

1.643

36

0.045639

2.49775

39


99

LAMPIRAN AB

Hasil Uji Statistik Kekerasan Tablet Antar Formula Batch 2 Anova: Single Factor SUMMARY Groups Column 1 Column 2 Column 3

Count 10 10 10

Sum 136.3 133.9 139.1

Average 13.63 13.39 13.91

Variance 0.149 0.269889 0.025444

10

139.9

13.99

0.032111

MS 0.752 0.119111

F 2.313433

Column 4


SS 2.256 4.288

Total

6.544

df 3 36

P-value 0.07149

39


100

F crit 2.8662

LAMPIRAN AC

Hasil Uji Statistik Kekerasan Tablet Antar Formula Batch 3 Anova: Single Factor SUMMARY Groups Column 1 Column 2 Column 3 Column 4

Count 10 10 10 10

Sum 135.1 136.7 139.1 140.2


1.59475 2.535

3 36

Total

4.12975

39

SS

Average 13.51 13.67 13.91 14.02

Variance 0.092111 0.131222 0.038778 0.019556

MS

F

Pvalue

F crit

0.531583 0.070417

2.549112

0.0704

2.8662

df


101

LAMPIRAN AD

Hasil Uji Statistik Kerapuhan Tablet Formula A Antar Batch Anova: Single Factor SUMMARY Groups Column 1 Column 2 Column 3

ANOVA Source of Variation Between Groups

Count 3 3 3

SS

Sum 0.567987 0.567987 0.567987

Average 0.189328 0.189328 0.189328

Variance 5.21E-08 5.21E-08 5.21E-08

df

MS -8.47E22 5.2099E08

F

Within Groups

0 3.1259E07

2 6

Total

3.1259E07

8

Pvalue

F crit

1

5.1432

-5.6E-16


102

LAMPIRAN AE

Hasil Uji Statistik Kerapuhan Tablet Formula B Antar Batch Anova: Single Factor SUMMARY Groups Column 1 Column 2 Column 3

Count 3 3 3

Sum 0.5679 0.5679 0.5679

Average 0.189328 0.189328 0.189328

Variance 5.21E-08 5.21E-08 5.21E-08

df

MS -8.47E22 5.2099E08

F


0 3.1259E07

6

Total

3.1259E07

8

SS

2

Pvalue

F crit

1

5.1432

-5.6E-16


103

LAMPIRAN AF

Hasil Uji Statistik Kerapuhan Tablet Formula C Antar Batch Anova: Single Factor SUMMARY Groups Column 1 Column 2

Count 3 3

Sum 0.550659 0.550659

Average 0.183553 0.183553

Variance 1.52E-06 1.52E-06

Column 3

3

0.550659

0.183553

1.52E-06

df

MS

F

2

-8.5E-22

-5.6E-16

6

1.52E-06


SS -1.7E21 9.09E06

Total

9.09E06

Pvalue

F crit

1

5.1432

8


104

LAMPIRAN AG

Hasil Uji Statistik Kerapuhan Tablet Formula D Antar Batch Anova: Single Factor SUMMARY Groups Column 1 Column 2 Column 3


Count 3 3 3

SS

Sum 0.5513 0.5513 0.5513

Average 0.183769 0.183769 0.183769

df

MS

1.69E-21

2

8.47E-22

1.08E-05

6

1.8E-06

1.08E-05

8

Variance 1.8E-06 1.8E-06 1.8E-06

F 4.71E16

Pvalue

F crit

1

5.1432


105

LAMPIRAN AH

Hasil Uji Statistik Kerapuhan Tablet Antar Formula Batch 1 Anova: Single Factor SUMMARY Groups Column 1 Column 2 Column 3 Column 4

Count 3 3 3 3


SS 7.25E05 6.82E06

Total

7.93E05

Sum 0.550949 0.567987 0.550659 0.551306

Average 0.18365 0.189329 0.183553 0.183769

Variance 4.64E-08 5.21E-08 1.52E-06 1.8E-06

MS

F

Pvalue

F crit

3

2.42E-05

2.321472

0.070

4.0661

8

8.53E-07

df

11


106

LAMPIRAN AI Hasil Uji Statistik Kerapuhan Tablet Antar Formula Batch 2 Anova: Single Factor SUMMARY Groups Column 1 Column 2 Column 3 Column 4

Count 3 3 3

Sum 0.550949 0.567987 0.550659

Average 0.18365 0.189329 0.183553

Variance 4.64E-08 5.21E-08 1.52E-06

3

0.551306

0.183769

1.8E-06

df

MS

F

3

2.42E-05

2.321472

8

8.53E-07


SS 7.25E05 6.82E06

Total

7.93E05

P-value

F crit

0.070

4.0661

11


107

LAMPIRAN AJ

Hasil Uji Statistik Kerapuhan Tablet Antar Formula Batch 3 Anova: Single Factor SUMMARY Groups Column 1 Column 2 Column 3 Column 4


Count 3 3 3 3

SS

Sum 0.550949 0.567987 0.550659 0.551306

Average 0.18365 0.189329 0.183553 0.183769

Variance 4.64E-08 5.21E-08 1.52E-06 1.8E-06

df

MS

F

Pvalue

F crit

2.321472

0.070

4.0661

7.25E-05

3

2.42E-05

6.82E-06

8

8.53E-07

7.93E-05

11


108

LAMPIRAN AK

Hasil Uji Statistik Penetapan Kadar Tablet Formula A Antar Batch Anova: Single Factor SUMMARY Groups Column 1 Column 2 Column 3


Count 3 3 3

SS

Sum 299.2556 301.5138 301.1747

Average 99.75187 100.5046 100.3916

Variance 0.989415 0.481305 1.086122

df

MS

F

Pvalue

F crit

0.579963

0.5884

5.1432

0.988583

2

0.494291

5.113684

6

0.852281

6.102267

8


109

LAMPIRAN AL

Hasil Uji Statistik Penetapan Kadar Tablet Formula B Antar Batch Anova: Single Factor SUMMARY Groups Column 1 Column 2 Column 3

Count 3 3 3

Sum 295.7052 296.6049 300.7618

Average 98.56841 98.86832 100.2539

Variance 0.239205 0.014562 0.940765

df

MS

F

Pvalue

F crit

5.091291

0.0759

5.1432


4.85083

2

2.425415

2.389065

6

0.398177

Total

7.239895

8

SS


110

LAMPIRAN AM

Hasil Uji Statistik Penetapan Kadar Tablet Formula C Antar Batch Anova: Single Factor SUMMARY Groups Column 1 Column 2 Column 3


Count 3 3 3

SS

Sum 296.2903 298.8141 298.8093

Average 98.76344 99.60471 99.60311

Variance 0.076254 0.770921 1.242892

df

MS

F

Pvalue

F crit

1.013945

0.4174

5.1432

1.412808

2

0.706404

4.180134

6

0.696689

5.592943

8


111

LAMPIRAN AN

Hasil Uji Statistik Penetapan Kadar Tablet Formula D Antar Batch Anova: Single Factor SUMMARY Groups Column 1 Column 2 Column 3


Count 3 3 3

SS

Sum 299.4608 301.8156 298.5046

Average 99.82027 100.6052 99.50154

Variance 1.212087 0.072452 1.42146

df

MS

F

Pvalue

F crit

1.073071

0.3995

5.1432

1.935818

2

0.967909

5.411996

6

0.901999

7.347814

8


112

LAMPIRAN AO

Hasil Uji Statistik Penetapan Kadar Tablet Antar Formula Batch 1 Anova: Single Factor SUMMARY Groups Column 1 Column 2 Column 3 Column 4


Count 3 3 3 3

SS

Sum 299.2556 295.7052 296.2903 299.4608

Average 99.75187 98.56841 98.76344 99.82027

Variance 0.989415 0.239205 0.076254 1.212087

df

MS

F

Pvalue

F crit

2.027984

0.188

4.0661

3.828267

3

1.276089

5.033922

8

0.62924

8.862188

11


113

LAMPIRAN AP



Count 3 3 3 3

SS

Sum 301.5138 296.6049 298.8141 301.8156

Average 100.5046 98.86832 99.60471 100.6052

Variance 0.481305 0.014562 0.770921 0.072452

df

MS

F

Pvalue

F crit

4.016449

0.0693

4.0661

6.043101

3

2.014367

2.678479

8

0.33481

8.72158

11


114

LAMPIRAN AQ



Count 3 3 3 3

SS

Sum 301.1747 300.7618 298.8093 299.6354

Average 100.3916 100.2539 99.60311 99.87848

Variance 1.086122 0.940765 1.242892 0.748171

df

MS

F

Pvalue

F crit

0.384325

0.7673

4.0661

1.158149

3

0.38605

8.035901

8

1.004488

9.19405

11


115

LAMPIRAN AR

Hasil Uji Statistik % ED360 PERHITUNGAN ANAVA (Uji Statistik % ED360 ) Anova: Single Factor SUMMARY Sum

Average

Column 1

Groups

3

196.26

65.421

Column 2

3

155.126

51.708

0.001

Column 3

3

142.2

47.423

0.0126

Column 4

3

117.914

39.304

0.0069

df

MS


Count

SS 1074.162

3

358.054

0.671547

8

0.08394

Variance 0.3150

F

Pvalue

F crit

4265.423 3.7E-13

4.066

Total 1074.834 11 keterangan: F hitung>F tabel (0,05) sehingga H ditolak dan ada perbedaan yang bermakna antar formula HSD =

0.6562 FA Mean

formula A

65.4216

formula B

51.7088

formula C

47.4233

Formula D

39.3047

FB

65.4216

51.7088

0

13.71278 0

*

FC

FD

47.423

39.3047

17.998

*

26.1169

*

4.2855

*

12.4041

*

8.11861

*

0

Keterangan

116

LAMPIRAN AS

Hasil Uji Statistik % Obat Terlepas Anova: Single Factor SUMMAR Y Groups

Count

Sum

Average

Variance

Column 1

3

298.48

99.493

0.1980

Column 2

3

256.16

85.387

0.1758

Column 3

3

240.59

80.197

0.0037

Column 4

3

210.58

70.193

0.0058


SS

df

MS

1340.767

3

446.92

0.766867

8

0.0959

F

Pvalue

F crit

4662.322

3E-13

4.066181

Total 1341.534 11 keterangan: F hitung>F tabel (0,05) sehingga H ditolak dan ada perbedaan yang bermakna antar formula HSD =

0.701267 FA Mean

formula A

99.493

formula B

85.386

formula C

80.196

Formula D

70.193

FB

99.493

85.386

0

14.106 0

FC

FD

80.196 *

70.19333

19.296

*

29.3

*

5.19

*

15.19333

*

10.00333

*

0

Keterangan * : Perbedaannya signifikan, karena selisihnya > HSD(5%) TS : Perbedaannya tidak signifikan, karena selisihnya < HSD(5%)

117

LAMPIRAN AT

Uji F Kurva Baku REPLIKASI 1 KONSENTRASI

ABSORBANSI

1.53 4.07 6.11 8.14 10.18 30.03

0.011 0.029 0.044 0.054 0.075 0.213

REPLIKASI 2 KONSENTRASI 1.52 4.04 6.06 8.08 10.10 29.80

ABSORBANSI 0.012 0.028 0.042 0.052 0.074 0.208

REPLIKASI 3 KONSENTRASI

ABSORBANSI

1.54 4.10 6.14 8.19 10.24 30.21

0.014 0.033 0.049 0.057 0.079 0.232 ΣX2 226.1777 222.6368 228.8517 677.6663

Regresi I Regresi II Regresi III

SSc=

X2

Y2

XY

2.331 16.581 37.307 66.324 103.632 226.177

0.000121 0.000841 0.001936 0.002916 0.005625 0.011439

0.0168 0.1181 0.2688 0.4398 0.7635 1.6069

X2 2.295 16.321 36.723 65.286 102.010 222.636

Y2 0.000144 0.000784 0.001764 0.002704 0.005476 0.010872

XY 0.0182 0.1131 0.2545 0.4202 0.7474 1.5534

Y2

XY

2.359 16.777 37.748 67.108 104.857 228.851

0.000196 0.001089 0.002401 0.003249 0.006241 0.013176

0.0215 0.1352 0.3011 0.4669 0.8090 1.7336

ΣXY 1.606 1.553 1.733 4.893

Σ Y2 0.011439 0.010872 0.013176 0.035487

N 5 5 5

X2

0.028265 267

F= 3.2929

< Ftabel0,05(2;12) 3,89

118

SSi 0.0043 0.0039 0.0056 0.0138

RDF 4 4 4

LAMPIRAN AU LOD dan LOQ y

ŷ

y-ŷ

(y-ŷ)2

0.011

0.01128

-0.00028

0.0000001

0.029

0.02948

-0.00048

0.0000002

0.044

0.04404

-0.00004

0.0000000

0.054

0.05860

-0.00460

0.0000212

0.075

0.07316

0.00184

0.0000034

Σ(y-ŷ)2

0.0000248

SB

0.0000143

y

ŷ

y-ŷ

(y-ŷ)2

0.012

0.01043

0.00157

0.0000025

0.028

0.02801

-0.00001

0.0000000

0.042

0.04208

-0.00008

0.0000000

0.052

0.05614

-0.00414

0.0000171

0.074

0.07020

0.00380

0.0000144

Σ(y-ŷ)2

0.0000340

SB

0.0000196

y

ŷ

y-ŷ

(y-ŷ)2

0.014

0.00788

0.00612

0.0000375

0.033

0.02621

0.00679

0.0000460

0.049

0.04088

0.00812

0.0000659

0.057

0.05555

0.00145

0.0000021

0.079

0.07022

0.00878

0.0000770

Σ(y-ŷ)2

0.0002285

SB

0.0001319

LOD

y=yB+3SB=

-0.0003

X= LOQ

LOD

0.006

y=yB+10SB=

-0.0002

X=

0.0201

y=yB+3SB=

0.0002

X= LOQ

LOD

0.008

y=yB+10SB=

0.0003

X=

0.0282

y=yB+3SB=

0.0035

X= LOQ

0.055

y=yB+10SB=

0.0044

X=

0.1842

Rata-rata

119

LOD=

0.023

ppm

LOQ=

0.077

ppm

LAMPIRAN A. Hasil Uji Mutu Fisik Granul

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