LAMPIRAN
Universitas Sumatera Utara
1. Alat dan Bahan Alat
Botol Ukuran 250 ml
Mikroskop Cahaya
Aerator
Bohlam 5 Watt
Haemocytometer
Akuarium Mini
Universitas Sumatera Utara
Termometer
Corong
Pipet Tetes
pH Meter
Tisu
Jarum Suntik
Universitas Sumatera Utara
Kertas Label
Spidol
Bahan
Vitamin B Kompleks Cair
Ragi Roti
Ampas Tahu
Universitas Sumatera Utara
Aquadest
Alkohol 70 %
Universitas Sumatera Utara
2.
Prosedur Penelitian
Bibit dari Alam
Proses Pemasukan Bibit Yang Sudah Dikultur
Pemberian Pakan Ragi Roti dan Vitamin B Kompleks
Universitas Sumatera Utara
Media Uji
Tampilan Media Siap Untuk Diuji
Universitas Sumatera Utara
3.
Rotifera (Brachionus sp)
Universitas Sumatera Utara
4. Tabel Pengamatan Pertumbuhan Harian Brachionus sp Perlakuan
A (Fitoplankton)
B (Ampas Tahu)
C (Ragi Roti)
D (Vit. B Kompleks)
Pengamatan
Ulangan
1 2 3 Jumlah Rata-rata 1 2 3 Jumlah Rata-rata 1 2 3 Jumlah Rata-rata 1 2 3 Jumlah Rata-rata
Awal
1 (Hari ke-2)
2 (Hari ke-4)
3 (Hari k3-6)
4 (Hari k3-8)
5 (Hari ke-10)
0.001 0.001 0.001 0.003 0.001 0.001 0.001 0.001 0.003 0.001 0.001 0.001 0.001 0.003 0.001 0.001 0.001 0.001 0.003 0.001
2 1 1 4 1.33 2 3 3 8 2.67 1 0.3 0.3 1.6 0.53 2 2 2 6 2.00
2 1 1 4 1.33 12 15 10 37 12.33 1 0.3 1 2.3 0.77 15 25 10 50 16.67
2 1 2 5 1.67 22 16 15 53 17.67 5 1 1 7 2.33 5 5 5 15 5.00
2 2 2 6 2.00 60 18 25 103 34.33 40 30 20 90 30.00 3 5 80 88 29.33
1 1 1 3 1.00 50 15 20 85 28.33 30 20 15 65 21.67 2 2 60 64 21.33
Universitas Sumatera Utara
5. Tabel Hasil Pertumbuhan Populasi dan Pertumbuhan Harian Brachionus sp pada Perlakuan
Pertumbuhan Populasi Brachionus sp Perlakuan
I 8 96 47 25
A (Fitoplankton) B ( Ampas Tahu) C ( Ragi Roti) D ( Vit. B Kompleks )
Ulangan II III 5 6 52 53 31.6 22.3 37 97
Jumlah
Rata-rata
19 201 100.9 159
6.33 67 33.63 53
Pertumbuhan Harian Populasi Brachionus sp Pada Setiap Perlakuan. Media Perlakuan
Waktu Pengamatan Fitoplankton
Ampas Tahu
Ragi Roti
Vitamin B Kompleks
Hari Pertama
0.001
0.001
0.001
0.001
Hari Ke-2
1.33
2.67
0.53
2.00
Hari Ke-4
1.33
12.33
0.77
16.67
Hari Ke-6
1.67
17.67
2.33
5.00
Hari Ke-8
2.00
34.33
30.00
29.33
Hari ke 10
1.00
18.33
15.00
9.66
Universitas Sumatera Utara
6. Tabel Analisis Anova Sidik Ragam Pertumbuhan Populasi Rotifera (Brachionus sp)
Perlakuan A (Fitoplankton) B ( Ampas Tahu) C ( Ragi Roti) D ( Vit. B Kompleks ) Jumlah Rata-rata
I 8 96 47 25 176 44
Ulangan II 5 52 31.6 37 125.6 31.4
III 6 53 22.3 97 178.3 44.575
∑
Ẋ
19 201 100.9 159 479.9
6.33 67 33.63 53 39.99
DB Total
= Jumlah seluruh observasi- 1 = n – 1 = 12 – 1 = 11
DB Perlakuan
= Banyaknya perlakuan – 1 = 4 – 1 = 3
DB Galat
= DB total - DB perlakuan = t (r –1) = (Jumlah perlakuan) (jumlah ulangan – 1) = 11 – 3 = 8
Faktor Koreksi (FK)
= (GT)2 = (479,9)2 = 230304,01 = 19192,00 n 12 12
JK Total
= (n-observasi total )2 – FK = {(8)2 + (5)2 + (6)2 + (96)2 + (52)2 + (53)2 + (47)2 + (31,6)2
+(22,3)2 +(25)2+ (37)2 + (97)2 } –
19192,00 = 10769,85
JK Perlakuan
= ∑ (total perlakuan)2 – FK r = (19)2 + (201)2 + (100.9)2 + (159)2 – 19192,00 3 = 6215,9366
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JK Galat
= JK Total – JK Perlakuan = 10769,85 – 6215,9366 = 4553,91
KT Perlakuan
= JK Perlakuan = 6215,9366 = 2071.9788 DB Perlakuan 3
KT Galat
= JK Galat = 4553,91 = 569.2387 T (r - 1) 8
F Hitung Perlakuan
= KT Perlakuan = 2071.9788 KT Galat
F Hitung Ulangan
= KT Ulangan KT Galat
JK
= 221.78083
Sumber Variasi
DB
KT
PERLAKUAN
3
6215.9366 2071.9788
ULANGAN GALAT TOTAL
2 8 11
443.56167 221.78083 4553.91 569.2387 10769.85
F hit 3.63991204 0.38960954
F tabel 5% 4.76
Universitas Sumatera Utara
1% 9.78
7. Hasil Analisis Anova Menggunakan Program SPSS 17
Correlations Notes Output Created
20-Nov-2013 09:50:19
Comments Input
Active Dataset
DataSet0er
Filter
<none>
Weight
<none>
Split File
<none>
N of Rows in Working Data File Missing Value Handling
Definition of Missing
4 User-defined missing values are treated as missing.
Cases Used
Statistics for each pair of variables are based on all the cases with valid data for that pair.
Syntax
CORRELATIONS /VARIABLES=Fitoplankton AmpasTahu RagiRoti VitaminBKompleks /PRINT=TWOTAIL NOSIG /STATISTICS DESCRIPTIVES /MISSING=PAIRWISE.
Resources
Processor Time
0:00:00.000
Elapsed Time
0:00:00.032
[DataSet0] Descriptive Statistics Mean
Std. Deviation
N
Fitoplankton
6.33
1.528
3
Ampas Tahu
67.00
25.120
3
Ragi Roti
33.633
12.4749
3
Vit. B Kompleks
53.000
38.5746
3
Universitas Sumatera Utara
Correlations Fitoplankton Fitoplankton
Pearson Correlation
Ampas Tahu 1
.755
-.339
.200
.455
.780
3
3
3
3
Pearson Correlation
.951
1
.920
-.613
Sig. (2-tailed)
.200
.256
.580
N
N Ragi Roti
3
3
3
3
Pearson Correlation
.755
.920
1
-.873
Sig. (2-tailed)
.455
.256
3
3
3
3
-.339
-.613
-.873
1
.780
.580
.324
3
3
3
N Vit. B Kompleks
Pearson Correlation Sig. (2-tailed) N
REGRESSION LISTWISE
Vit. B Kompleks
.951
Sig. (2-tailed)
Ampas Tahu
Ragi Roti
/DESCRIPTIVES MEAN STDDEV CORR SIG N
.324
/MISSING
/STATISTICS COEFF OUTS R ANOVA COLLIN TOL CHANGE ZPP
/CRITERIA=PIN(.05) POUT(.10)
/NOORIGIN
/DEPENDENT Fitoplankton
/METHOD=ENTER AmpasTahu RagiRoti VitaminBKompleks
/RESIDUALS
HIST(ZRESID) NORM(ZRESID).
Universitas Sumatera Utara
3
Regression
Notes Output Created
20-Nov-2013 09:51:15
Comments Input
Active Dataset
DataSet0
Filter
<none>
Weight
<none>
Split File
<none>
N of Rows in Working Data File Missing Value Handling
Definition of Missing
4 User-defined missing values are treated as missing.
Cases Used
Statistics are based on cases with no missing values for any variable used.
Syntax
REGRESSION /DESCRIPTIVES MEAN STDDEV CORR SIG N /MISSING LISTWISE /STATISTICS COEFF OUTS R ANOVA COLLIN TOL CHANGE ZPP /CRITERIA=PIN(.05) POUT(.10) /NOORIGIN /DEPENDENT Fitoplankton /METHOD=ENTER AmpasTahu RagiRoti VitaminBKompleks /RESIDUALS HIST(ZRESID) NORM(ZRESID).
Resources
Processor Time
0:00:00.063
Elapsed Time
0:00:00.077
Memory Required Additional Memory Required for
1956 bytes 640 bytes
Residual Plots
[DataSet
Universitas Sumatera Utara
Warnings For the final model with dependent variable Fitoplankton, the variance- covariance matrix is singular. Influence statistics cannot be computed.
Descriptive Statistics Mean
Std. Deviation
N
Fitoplankton
6.33
1.528
3
Ampas Tahu
67.00
25.120
3
Ragi Roti
33.633
12.4749
3
Vit. B Kompleks
53.000
38.5746
3
Correlations Fitoplankton Pearson Correlation
N
Ragi Roti
Vit. B Kompleks
Fitoplankton
1.000
.951
.755
-.339
Ampas Tahu
.951
1.000
.920
-.613
Ragi Roti
.755
.920
1.000
-.873
-.339
-.613
-.873
1.000
Fitoplankton
.
.100
.228
.390
Ampas Tahu
.100
.
.128
.290
Ragi Roti
.228
.128
.
.162
Vit. B Kompleks
.390
.290
.162
.
Fitoplankton
3
3
3
3
Ampas Tahu
3
3
3
3
Ragi Roti
3
3
3
3
Vit. B Kompleks
3
3
3
3
Vit. B Kompleks Sig. (1-tailed)
Ampas Tahu
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Model Summaryb Change Statistics
Std. Error of the Model
R
R Square
1.000a
1
Adjusted R Square
1.000
Estimate
.
R Square Change .
1.000
F Change
df1 .
df2 2
Sig. F Change 0
.
a. Predictors: (Constant), Vit. B Kompleks, Ampas Tahu b. Dependent Variable: Fitoplankton Model 1
Variables Entered Variables Removed Vit. B Kompleks,
Method
. Enter
a
Ampas Tahu
a. Tolerance = .000 limits reached.
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ANOVAb Model 1
Sum of Squares Regression Residual Total
df
Mean Square
F
4.667
2
2.333
.000
0
.
4.667
2
Sig. .a
.
a. Predictors: (Constant), Vit. B Kompleks, Ampas Tahu b. Dependent Variable: Fitoplankton
Coefficientsa Standardized Unstandardized Coefficients Model
B
1(Constant)
Coefficients
Std. Error
Correlations
Beta
.663
.000
Ampas Tahu
.072
.000
Vit. B Kompleks
.015
.000
t
Sig.
Zero-order
Partial
Collinearity Statistics Part
Tolerance
VIF
.
.
1.191
.
.
.951
1.000
.941
.624
1.602
.390
.
.
-.339
1.000
.308
.624
1.602
a. Dependent Variable: Fitoplankton
Universitas Sumatera Utara
Excluded Variablesb Collinearity Statistics Minimum Model 1
Beta In
t .a
Ragi Roti
Sig. .
Partial Correlation .
Tolerance .
VIF
.000
Tolerance .
.000
a. Predictors in the Model: (Constant), Vit. B Kompleks, Ampas Tahu b. Dependent Variable: Fitoplankton
Collinearity Diagnosticsa Variance Proportions
Dimensio Model
n
Eigenvalue
Condition Index
(Constant)
Ampas Tahu
Vit. B Kompleks
1
1
2.701
1.000
.00
.01
.02
2
.281
3.101
.00
.07
.36
3
.018
12.114
.99
.93
.62
a. Dependent Variable: Fitoplankton
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Residuals Statisticsa Minimum
Maximum
Mean
Std. Deviation
N
Predicted Value
5.00
8.00
6.33
1.528
3
Residual
.000
.000
.000
.000
3
-.873
1.091
.000
1.000
3
.
.
.
.
0
Std. Predicted Value Std. Residual a. Dependent Variable: Fitoplankton
Universitas Sumatera Utara
Universitas Sumatera Utara