7.
LAMPIRAN
Lampiran 1. Kurva Standar Protein Larutan Bardfrod Commasive blue ditimbang sebanyak 0,01 gram kemudian dilarutkan ke dalam 5 ml etanol 95% dan ditambah dengan 10 ml asam fosfor. Larutan selanjutnya disaring dengan menggunakan kertas saring dan disimpan dalam botol gelap pada suhu rendah. Sebelum digunakan, larutan harus diencerkan terlebih dahulu sebanyak lima kali (Bollag & Stuart, 1991) Pembuatan Kurva Standar Bovine Serum Albumin (BSA) ditimbang sebanyak 100 mg kemudian dilarutkan ke dalam 100 ml ddH2O dan larutan tersebut dinamakan larutan stok BSA 1 mg/ml. Selanjutnya, larutan stok BSA dibuat seri pengenceran larutan standar dengan konsentrasi 0 mg/ml, 0,1 mg/ml, 0,2 mg/ml, 0,3 mg/ml, 0,4 mg/ml, 0,5 mg/ml, 0,6 mg/ml, 0,7 mg/ml, 0,8 mg/ml, 0,9 mg/ml, dan 1 mg/ml. Dari masing-masing konsentrasi larutan standar diambil sebanyak 0,1 ml, lalu ditambahkan 5 ml larutan Bradford. Setelah itu, larutan didiamkan selama 5 menit, dan diukur absorbansinya pada panjang gelombang 595 nm (Bradford, 1976).
Gambar 19. Kurva Standar Protein
49
50
Lampiran 2. Komposisi Reagen Elektroforesis Protein
Larutan Stok (Stock Solutions)
Bahan-bahan yang digunakan dalam pembuatan larutan stok adalah 2M Tris-HCl (pH 8,8) 100 ml, 1 Tris-HCl (pH 6,8) 100 ml, 10% SDS 100 ml, 50% gliserol 100 ml, dan 1% bromophenol blue 10 ml.
Larutan Kerja (Working Solutions) Nama Larutan
Larutan A (Acrylamide Stock Solution), 100 ml 30% acrylamide, 0,8% bisacrylamide Larutan B (4x Separating Gel Buffer), 100 ml Larutan C (4x Stacking Gel Buffer), 100 ml 10% Ammonium Persulphate (APS) Buffer Elektroforesis
Sampel Buffer
Komposisi 29,2 gram acrylamide 0,8 gram bis-acrylamide
75 ml 2M Tris-HCl (pH 8,8) --- 1,5 M 4 ml 10% SDS --- 0,4 % 21 ml ddH2O 50 ml 1M Tris-HCl (pH 6,8) --- 0,5 M 4 ml 10% SDS --- 0,4 % 46 ml ddH2O 0,5 gram APS 5 ml DDH2O 3 gr Tris --- 25Mm 14,4 gram glycine --- 192Mm 1 gram SDS --- 0,1% ddH2O sampai 1 liter 3,028 gram Tris 0,25 ml larutan C 0,4 ml 10% SDS 25 ml gliserol 0,2 mg bromophenol blue 0,031 gram Dithiothreitol (DTT)
Larutan 12,5% Separating Gel Buffer (dalam 10 ml)
Larutan Larutan acrylamide 4x Separating gel buffer 10% SDS ddH2O 10% Ammonium Persulphate (APS) TEMED
Jumlah 4,2 ml 2,5 ml 100 µl 3,2 ml 50 µl 3,4 µl
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Larutan 4x Stacking Gel Buffer (dalam 10 ml) Larutan Jumlah Larutan acrylamide 2,66 ml 4x stacking gel buffer 2,5 ml 10% SDS 0,1 ml ddH2O 6 ml 10% Ammonium Persulphate (APS) 50 µl TEMED 5 µl
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Lampiran 3. Kurva Standar BSA (Bovine Serum Albumin) Low Moleculer Weight
Gambar 20. Kurva Standar BSA (Bovine Serum Albumin) Low Moleculer Weight
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Lampiran 4. Hasil Pengolahan SPSS Tingkat Kekerasan (Hardness) pada Adonan Tepung Terigu
Hard ness a
Duncan
Perlakuan Adonan Tpeung Terigu Kontrol Adonan Tepung Terigu + Jahe 1,5% Adonan Tepung Terigu + Jahe 3% Sig.
N
1 6
Subset f or alpha = .05 2 3
183,0533
6
220,4083
6
310,7017 1,000
1,000
Means f or groups in homogeneous subsets are display ed. a. Uses Harmonic Mean Sam ple Size = 6,000.
1,000
54
Lampiran 5. Hasil Pengolahan SPSS Karakteristik Fisik Roti Kukus a.
Tingkat Kekerasan (Hardness)
Roti Kukus Jahe 0% Hard ness Roti Ku kus Jahe 0% a,b
Duncan
Tahapan Proof ing Mixing St eam ing Sig.
N 6 6 6
1 115,9317
Subset 2
3
176,0700 1,000
1,000
370,4967 1,000
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = 40,777. a. Uses Harmonic Mean Sample Size = 6,000. b. Alpha = , 05.
Roti Kukus Jahe 1,5% Hardness Roti Kukus Jahe 1,5% a,b
Duncan
Tahapan Proof ing Mixing St eam ing Sig.
N 6 6 6
1 143,8850
Subset 2
3
217,5817 1,000
1,000
712,6117 1,000
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = 261,490. a. Uses Harmonic Mean Sample Size = 6,000. b. Alpha = , 05.
Roti Kukus Jahe 3% Hardness Roti Kukus Jahe 3% a,b
Duncan
Tahapan Proof ing Mixing St eam ing Sig.
N 6 6 6
1 168,1267
Subset 2
3
265,6117 1,000
1,000
899,9483 1,000
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = 57,050. a. Uses Harmonic Mean Sample Size = 6,000. b. Alpha = , 05.
55
Mixing Hardness Mixing a,b
Duncan
Perlakuan Roti Kukus Kont rol Roti Kukus Jahe 1,5% Roti Kukus Jahe 3% Sig.
N 6 6 6
1 176,0700
Subset 2
3
217,5817 1,000
1,000
265,6117 1,000
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = 56,683. a. Uses Harmonic Mean Sample Size = 6,000. b. Alpha = , 05.
Proofing Hardness Proo fing a,b
Duncan
Perlakuan Roti Kukus Kont rol Roti Kukus Jahe 1,5% Roti Kukus Jahe 3% Sig.
N 6 6 6
1 115,9317
Subset 2
3
143,8850 1,000
1,000
168,1267 1,000
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = 22,232. a. Uses Harmonic Mean Sample Size = 6,000. b. Alpha = , 05.
Steaming Hardness Steaming a,b
Duncan
Perlakuan Roti Kukus Kont rol Roti Kukus Jahe 1,5% Roti Kukus Jahe 3% Sig.
N 6 6 6
1 370,4967
Subset 2 712,6117
1,000
1,000
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = 280,403. a. Uses Harmonic Mean Sample Size = 6,000. b. Alpha = , 05.
3
899,9483 1,000
56
Warna
b.
L* L a
Duncan
Perlakuan Roti Kukus Jahe 3% Roti Kukus Jahe 1,5% Roti Kukus Kont rol Sig.
Subset f or alpha = . 05 1 2 3 75,1567 76,3233 84,1850 1,000 1,000 1,000
N 6 6 6
Means f or groups in homogeneous subset s are display ed. a. Uses Harmonic Mean Sample Size = 6,000.
a* a a
Duncan
Perlakuan Roti Kukus Kont rol Roti Kukus Jahe 1,5% Roti Kukus Jahe 3% Sig.
Subset f or alpha = . 05 1 2 3 -2,7733 -2,3667 -,8083 1,000 1,000 1,000
N 6 6 6
Means f or groups in homogeneous subset s are display ed. a. Uses Harmonic Mean Sample Size = 6,000.
b* b a
Duncan
Perlakuan Roti Kukus Kont rol Roti Kukus Jahe 3% Roti Kukus Jahe 1,5% Sig.
N 6 6 6
Subset f or alpha = . 05 1 2 3 20,4600 24,3767 27,7800 1,000 1,000 1,000
Means f or groups in homogeneous subset s are display ed. a. Uses Harmonic Mean Sample Size = 6,000.
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c.
Volume Pengembangan
Roti Kukus Jahe 0% Vo lume a,b
Duncan
Tahapan Mixing Proof ing St eam ing Sig.
N 6 6 6
1 31,2017
Subset 2
3
55,3967 1,000
1,000
81,6717 1,000
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = ,792. a. Uses Harmonic Mean Sample Size = 6,000. b. Alpha = , 05.
Roti Kukus Jahe 1,5% Vo lume a,b
Duncan
Tahapan Mixing Proof ing St eam ing Sig.
N 6 6 6
1 35,4583
Subset 2
3
47,7350 1,000
1,000
74,2233 1,000
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = ,761. a. Uses Harmonic Mean Sample Size = 6,000. b. Alpha = , 05.
Roti Kukus Jahe 3% Vo lume a,b
Duncan
Tahapan Mixing Proof ing St eam ing Sig.
N 6 6 6
1 39,4717
Subset 2
3
51,8533 1,000
1,000
77,4100 1,000
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = ,894. a. Uses Harmonic Mean Sample Size = 6,000. b. Alpha = , 05.
58
Mixing Volume a,b
Duncan
Perlakuan Roti Kukus Kont rol Roti Kukus Jahe 1,5% Roti Kukus Jahe 3% Sig.
N 6 6 6
1 31,2017
Subset 2
3
35,4583 1,000
1,000
39,4717 1,000
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = ,778. a. Uses Harmonic Mean Sample Size = 6,000. b. Alpha = , 05.
Proofing Volume a,b
Duncan
Perlakuan Roti Kukus Jahe 1,5% Roti Kukus Jahe 3% Roti Kukus Kont rol Sig.
N 6 6 6
1 47,7350
Subset 2
3
51,8533 1,000
1,000
55,3967 1,000
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = ,801. a. Uses Harmonic Mean Sample Size = 6,000. b. Alpha = , 05.
Steaming Volume a,b
Duncan
Perlakuan Roti Kukus Jahe 1,5% Roti Kukus Jahe 3% Roti Kukus Kont rol Sig.
N 6 6 6
1 74,2233
Subset 2 77,4100
1,000
1,000
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = ,868. a. Uses Harmonic Mean Sample Size = 6,000. b. Alpha = , 05.
3
81,6717 1,000
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Lampiran 6. Hasil Pengolahan SPSS Aktivitas Antioksidan pada Roti Kukus
Roti Kukus Jahe 0% Anti oksidan a,b
Duncan
Tahapan St eam ing Proof ing Mixing Sig.
N
1 2,9567
6 6 6
Subset 2
3
8,0750 1,000
1,000
11,4750 1,000
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = ,877. a. Uses Harmonic Mean Sample Size = 6,000. b. Alpha = , 05.
Roti Kukus Jahe 1,5% Anti oksidan a,b
Duncan
Tahapan St eam ing Proof ing Mixing Sig.
N
1 9,3483
6 6 6
Subset 2
3
13,8067 1,000
1,000
21,6717 1,000
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = 3,089. a. Uses Harmonic Mean Sample Size = 6,000. b. Alpha = , 05.
Roti Kukus Jahe 3% Anti oksidan a,b
Duncan
Tahapan St eaming Proof ing Mixing Sig.
N 6 6 6
1 18,7217
Subset 2
3
26,3800 1,000
1,000
34,1617 1,000
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = 1,153. a. Uses Harmonic Mean Sample Size = 6,000. b. Alpha = , 05.
60
Mixing Anti oksidan a,b
Duncan
Perlakuan Roti Kukus Kont rol Roti Kukus Jahe 1,5% Roti Kukus Jahe 3% Sig.
N 6 6 6
1 11,4750
Subset 2
3
21,6717 1,000
1,000
34,1617 1,000
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = 1,327. a. Uses Harmonic Mean Sample Size = 6,000. b. Alpha = , 05.
Proofing Anti oksidan a,b
Duncan
Perlakuan Roti Kukus Kont rol Roti Kukus Jahe 1,5% Roti Kukus Jahe 3% Sig.
N 6 6 6
1 8,0750
Subset 2
3
13,8067 1,000
1,000
26,3800 1,000
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = 2,604. a. Uses Harmonic Mean Sample Size = 6,000. b. Alpha = , 05.
Steaming Anti oksidan a,b
Duncan
Perlakuan Roti Kukus Kont rol Roti Kukus Jahe 1,5% Roti Kukus Jahe 3% Sig.
N 6 6 6
1 2,9567
Subset 2 9,3483
1,000
1,000
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = 1,188. a. Uses Harmonic Mean Sample Size = 6,000. b. Alpha = , 05.
3
18,7217 1,000
