LAMPIRAN
57
LAMPIRAN 1 PERHITUNGAN DOSIS 1. Perhitungan dosis pemberian Anthelmentik Albendazole 5% = 5 g/100ml = 50 gram/ml Dosis untuk mencit = 10 mg/kg BB Dosis untuk mencit BB 20 gram = =
= 0,04 ml/ekor 2. Perhitungan dosis pemberian Antibiotik (Clavamox®) Konsentrasi = 125 mg/ml = 25 mg/ml Dosis untuk anak-anak BB 25 kg = 1 mg/kg BB/hari
= 0,001 mg/g BB
Dosis untuk mencit = Dosis untuk mencit BB 20 gram =
= 0,0008 ml/ekor/hari
3. Perhitungan dosis pemberian Antiprotozoa (Flagyl®) Sediaan 500 mg/tablet Dosis untuk manusia BB 50 kg = 1500 mg/50 kg BB/hari = 30 mg/kg BB/hari
Dosis untuk mencit
= = 0,03 mg/g BB
Dosis untuk mencit BB 25 gram = 25 (gram) x 0,03 mg/g BB = 0,75 mg/ekor/hari
58
4.
Perhitungan Dosis Pemakaian Jintan Hitam Dosis Pemakaian = 1-2 sdm 3x sehari Dosis Preventif = =
Dosis kuratif
= 0,3 ml/kg x 2 sdm = 0,6 ml/kg x 0,6 x 3 = 0,054 ml ≈ 0,1 ml/ hari
= 2x Dosis Preventif = 0,2 ml/ hari
Dosis Hs madu = 3x Dosis Preventif = 0,3 ml/ hari
59
LAMPIRAN 2 JADWAL
Jadwal Pelaksanaan Penelitian Waktu Juni 2010
Kegiatan Mempersiapkan dan membersihkan Fasilitas Kandang Hewan Percobaan Bagian Patologi FKH IPB Persiapan peralatan perkandangan hewan coba seperti box kandang, alas kain, tempat makan, dan tempat minum
16 Agustus 2010
Mencit untuk hewan percobaan dimasukkan ke dalam kandang dan diistirahatkan selama 2 hari agar dapat beradaptasi dengan kandang
20 Agustus 2010
Pemberian antihelmintik pada mencit
23-27 Agustus 2010
Pemberian antibiotik pada mencit
28 Agustus 2010
Pemberian antihelmintik pada mencit
30 Agustus-3 September 2010
Pemberian antijamur pada mencit
16 September-16 November 2010
Pemberian ekstrak minyak jintan hitam dan kombinasi madu pada kelompok perlakuan
22 November 2010
Nekropsi mencit
29 November 2010
Trimming organ mencit
1-15 Desember 2010
Pembuatan preparat histopatologi
Januari -Februari 2011
Pengamatan preparat histopatologi
60
LAMPIRAN 3 HASIL ANALISIS DATA LIMFONODUS JANTAN Case Processing Summary Cases Included
Excluded
Total
N
Percent
N
Percent
N
Percent
Jumlah * Perlakuan
11
100.0%
0
.0%
11
100.0%
Luas * Perlakuan
11
100.0%
0
.0%
11
100.0%
Jumlah * Sampel
11
100.0%
0
.0%
11
100.0%
Luas * Sampel
11
100.0%
0
.0%
11
100.0%
Jumlah Luas * Perlakuan
Kontrol
Perlakuan
Jumlah
Luas
Mean
6.5000
71.5000
2
2
Std. Deviation
.70711
1.484921
Mean
6.6667
1.2167E2
3
3
Std. Deviation
2.88675
1.258311
Mean
4.0000
2.01002
3
3
Std. Deviation
.00000
3.119291
Mean
4.3333
3.06672
3
3
Std. Deviation
1.52753
6.806861
Mean
5.2727
1.84642
11
11
1.95402
9.732551
N
Pre
N
Ku
N
Madu
N
Total
N Std. Deviation
61
ANOVA Sum of Squares
df
Mean Square
F
Sig.
.409
3
.136
.147
.928
Within Groups
6.500
7
.929
Total
6.909
10
16.348
3
5.449
1.747
.244
Within Groups
21.833
7
3.119
Total
38.182
10
82972.712
3
27657.571
16.477
.001
Within Groups
11749.833
7
1678.548
Total
94722.545
10
Sampel Between Groups
Jumlah Between Groups
Luas
Between Groups
Jumlah Duncan Perlakuan
N
Subset for alpha = 0.05 1
Kuraftif
3
4.0000
Madu
3
4.3333
Kontrol
2
6.5000
Preventif
3
6.6667
Sig.
.143
Means for groups in homogeneous subsets are displayed.
62
Luas Duncan Subset for alpha = 0.05 Perlakuan
N
1
Kontrol
2
71.5000
Preventif
3
1.2167
Kuratif
3
Madu
3
Sig.
2
3
1.2167 2.0100 3.0667
.200
.060
1.000
Means for groups in homogeneous subsets are displayed.
63
LAMPIRAN 4 HASIL ANALISIS DATA LIMFONODUS BETINA Case Processing Summary Cases Included
Excluded
Total
N
Percent
N
Percent
N
Percent
Jumlah * Perlakuan
11
100.0%
0
.0%
11
100.0%
Luas * Perlakuan
11
100.0%
0
.0%
11
100.0%
Jumlah * Sampel
11
100.0%
0
.0%
11
100.0%
Luas * Sampel
11
100.0%
0
.0%
11
100.0%
Jumlah Luas * Perlakuan Perlakuan Kontrol
Jumlah
Luas
5.5000
78.0000
2
2
Std. Deviation
.70711
1.13137
Mean
6.6667
1.61002
3
3
Std. Deviation
1.52753
6.82862
Mean
6.0000
2.51332
3
3
Std. Deviation
2.64575
8.25671
Mean
6.6667
3.42672
3
3
Std. Deviation
3.51188
1.02632
Mean
6.2727
2.20092
11
11
2.14900
1.105252
Mean N
Pre
N
Ku
N
Madu
N
Total
N Std. Deviation
64
ANOVA Sum of Squares
df
Mean Square
F
Sig.
.409
3
.136
.147
.928
Within Groups
6.500
7
.929
Total
6.909
10
2.348
3
.783
.125
.942
Within Groups
43.833
7
6.262
Total
46.182
10
Between Groups
98857.576
3
32952.525
9.900
.007
Within Groups
23299.333
7
3328.476
Total
122156.909
10
Sampel Between Groups
Jumlah Between Groups
Luas
Jumlah Duncan Perlakuan
N
Subset for alpha = 0.05 1
Kontrol
2
5.5000
Ku
3
6.0000
Pre
3
6.6667
Madu
3
6.6667
Sig.
.624
Means for groups in homogeneous subsets are displayed.
65
Luas Duncan Subset for alpha = 0.05 Perlakuan
N
1
Kontrol
2
78.0000
Pre
3
1.6100
Ku
3
Madu
3
Sig.
2
3
1.6100 2.5133
2.5133 3.4267
.141
.114
.110
Means for groups in homogeneous subsets are displayed.
66
LAMPIRAN 5 HASIL ANALISIS DATA LIMPA JANTAN Case Processing Summary Cases Included
Excluded
Total
N
Percent
N
Percent
N
Percent
Jumlah * Perlakuan
11
100.0%
0
.0%
11
100.0%
Luas * Perlakuan
11
100.0%
0
.0%
11
100.0%
Jumlah * Sampel
11
100.0%
0
.0%
11
100.0%
Luas * Sampel
11
100.0%
0
.0%
11
100.0%
Jumlah Luas * Perlakuan Perlakuan Kontrol
Jumlah
Luas
6.5000
1.4250
2
2
Std. Deviation
2.12132
4.59619
Mean
7.3333
3.2500
3
3
Std. Deviation
1.15470
4.68615
Mean
6.0000
4.8767
3
3
Std. Deviation
2.64575
7.40968
Mean
6.3333
5.2333
3
3
Std. Deviation
1.52753
1.33088
Mean
6.5455
3.9027
11
11
1.69491
1.64331
Mean N
Pre
N Ku
N Madu
N Total
N Std. Deviation
67
ANOVA Sum of Squares
df
Mean Square
F
Sig.
.409
3
.136
.147
.928
Within Groups
6.500
7
.929
Total
6.909
10
2.894
3
.965
.261
.851
Within Groups
25.833
7
3.690
Total
28.727
10
Between Groups
217136.348
3
72378.783
9.576
.007
Within Groups
52909.833
7
7558.548
Total
270046.182
10
Sampel Between Groups
Jumlah Between Groups
Luas
Jumlah Duncan Perlakuan
Subset for alpha = 0.05
N
1 Ku
3
6.0000
Madu
3
6.3333
Kontrol
2
6.5000
Pre
3
7.3333
Sig. Means for groups in homogeneous subsets are displayed.
.471
68
Luas Duncan Subset for alpha = 0.05 Perlakuan
N
1
Kontrol
2
1.4250
Pre
3
3.2500
Ku
3
4.8767
Madu
3
Sig.
2
3
4.8767 5.2333
1.000
.068
Means for groups in homogeneous subsets are displayed.
.650
69
LAMPIRAN 6 HASIL ANALISIS DATA LIMPA BETINA Case Processing Summary Cases Included
Excluded
Total
N
Percent
N
Percent
N
Percent
Jumlah * Perlakuan
11
100.0%
0
.0%
11
100.0%
Luas * Perlakuan
11
100.0%
0
.0%
11
100.0%
Jumlah * Sampel
11
100.0%
0
.0%
11
100.0%
Luas * Sampel
11
100.0%
0
.0%
11
100.0%
Jumlah Luas * Perlakuan Perlakuan Kontrol
Jumlah
Luas
8.0000
1.1500
2
2
Std. Deviation
.00000
7.07107
Mean
6.3333
2.4533
3
3
Std. Deviation
2.08167
7.25282
Mean
6.0000
4.6833
3
3
Std. Deviation
1.00000
1.97791
Mean
6.0000
5.6767
3
3
Std. Deviation
3.46410
1.67112
Mean
6.4545
3.7036
11
11
2.01810
2.16232
Mean N
Pre
N Ku
N Madu
N Total
N Std. Deviation
70
ANOVA Sum of Squares
df
Mean Square
F
Sig.
.409
3
.136
.147
.928
Within Groups
6.500
7
.929
Total
6.909
10
6.061
3
2.020
.408
.752
Within Groups
34.667
7
4.952
Total
40.727
10
Between Groups
322898.545
3
107632.848
5.208
.033
Within Groups
144666.000
7
20666.571
Total
467564.545
10
Sampel Between Groups
Jumlah Between Groups
Luas
Jumlah Duncan Subset for alpha = 0.05 Perlakuan
N
1
Ku
3
6.0000
Madu
3
6.0000
Pre
3
6.3333
Kontrol
2
8.0000
Sig.
.357
Means for groups in homogeneous subsets are displayed.
71
Luas Duncan Subset for alpha = 0.05 Perlakuan
N
1
Kontrol
2
1.1500
Pre
3
2.4533
Ku
3
Madu
3
Sig.
2
3
2.4533 4.6833
4.6833 5.6767
.330
.116
Means for groups in homogeneous subsets are displayed.
.451
