SATURDAY, OCTOBER 3, 2015 HALL A | EVROPA 1 19.00 – 19.30 1.
Opening Chairmen: Doležel J., Kalina T.
Opening Lecture Robinson J.P. | West Lafayette, Indiana, USA
FOYER 19.30 - 21.30
Welcome Drink
SUNDAY, OCTOBER 4, 2015 HALL A | EVROPA 1 | PLENARY SESSIONS 8.30
Opening Ceremony
8.55 – 10.25
Future of Cell Analysis Chairmen: Souček K., Kalina T.
2.
Spectral Cytometry – Is This the Future or Is It Now? Robinson J.P. | West Lafayette, Indiana, USA
(35´)
3.
Single Cell Analysis on Microfluidic Devices Dittrich P. | Zürich, CH
(35´)
4.
Company Presentati on: Plati num Partner - Beckman Coulter Beckman Coulter Flow Cytometry in 2015 and Beyond Jaen O.
(20´)
10.25 – 10.45
Coffee Break
10.45 – 12.15
Cytometry Inspirati on Chairmen: Hofmanová J., Říhová L.
5.
Flow Cytometry Detection of Microparticles in Whole Blood: A Novel Approach Manganaro D. | Milano, IT
(35´)
6.
Challenges of Minimal Residual Disease Testing and its Promise for Multiple Myeloma Therapeutics Wallace P.K. | Buffalo, NY, USA
7.
Company Presentation: Gold Partner - BD Biosciences | I.T.A. - Intertact s.r.o. New Tools in Flow Cytometry for Research and Diagnostics (15´) Loehr A.
8.
Company Presentation: Silver Partner – Bio-Rad How the Biorad S3e Cell Sorter Simplifies the Science of Sorting (10´) Astruc C. | Business Development Manager CBBU
12.15 – 13.45
Lunch / Lunch Sessions
13.45 – 15.35
Cytometry Investigation of Mice and Man Chairmen: Průcha M., Drbal K.
9.
(35´)
Application of Flow Cytometry in the Diagnostics of Allergy Knol E. | Utrecht, NL
(35´)
10. The Immunophenome of the Mice - Integrative Multiparametric Approach Luche H. | Marseilles, FR
(35´)
Company Presentation: Silver Partner – Miltenyi Biotec GmbH | Biohem (10´) 11. REAfinityTM and Vio®Dye Antibodies Goczalik-Schuster I. Company Presentation: Silver Partner – Merck Millipore 12. Fast flow of Recent Merck Millipore Innovations for Flow Cytometry Kukla S.
(10´)
Company Presentation: Silver Partner – Accela 13. High-End Cytometric Solutions for Cancer Research Pasculli R.
(10´)
Company Presentation: Partner – Life Technologies Czech Republic 14. Attune NxT: The Next Generation Acoustic Flow Cytometer Brey A.
(8´)
15.35 – 15.55
Coffee Break
15.55 – 17.10
Back to Origins Chairmen: Eckschlager T., Kanderová V.
15. Toll-Like Receptors Expressed on Embryonic Macrophages Couple Inflammatory Signals to Iron Metabolism During Early Ontogenesis Balounová J., Vavrochová T., Dobešová M., Ballek O., Kolář M., Filipp D. | Prague, CZ
(20´)
16. The Transmembrane Protein EVI2B Regulates Hematopoietic (20´) Stem Cell Function Zjablovskaja P.1, Kardošová M.1, Brdička T1., Benoukraf T.2, Tenen D.2, Malissen B.3, Balaštík M.1, Hořejší V.1, Alberich-Jorda M.1 | 1Prague, CZ, 2Singapore, SG, 3 Marseille, FR Company Presentation: Partner – EXBIO Praha 17. EXBIO aneb výroba protilátek v Čechách Špryngar M.
(8)
Company Presentation: Partner – Biolegend 18. Effective Magnetic Cell Separation with MojoSort™ Fröhlich A.
(8´)
Company Presentation: Partner – Olympus 19. Scan R – Microscopic System for High Content Screening (8´) Jendrulek T. 17.00 – 17.10 Award Ceremony – The Best Cytometric Publication 2014
SUNDAY, OCTOBER 4, 2015 HALL P | OLOMOUC 1+2+3 17.10 – 18.10
Poster Presentati ons I Moderated poster walk for authors of odd-numbered posters (Posters P1, P3, P5 …)
MONDAY, OCTOBER 5, 2015 HALL P | OLOMOUC 1+2+3 17.05 – 18.00
Poster Presentati ons II Moderated poster walk for authors of even-numbered posters (Posters P2, P4, P6 …)
MONDAY, OCTOBER 5, 2015 HALL A | EVROPA 1 | PLENARY SESSION 8.30 – 10.35
Stories Told by Cells Chairmen: Doležel J., Vlková M.
20. Cytometry of Immunological Memory and Immunity Radbruch A. | Berlin, DE
(35´)
21. The Private Life of Plants: New Insights Provided by DNA Flow Cytometry Suda J. | Prague, CZ
(35´)
22. Application of Flow Cytometry in Protein and Metabolic Engineering (20´) Prokop Z.1, Fedr R.1, Buryška T.1, Dvořák P.1, Chrást L.1, Fischlechner M.2, Nikel P. I.3, Chaloupková R.1, Hollfelder F.4, de Lorenzo V.3, Souček K.1, Damborský J.1 | 1Brno, CZ, 2Southampton, UK, 3Cantoblanco-Madrid, ES, 4Cambridge, UK Company Presentati on: Partner – BIO-PORT Europe 23. eBioscience PrimeFlow™ RNA Assay – Simultaneous Detection of RNA and Proteins on Single Cell Level Hovorka O. Company Presentati on: Gold Partner – Scinti la 24. Spectral Analysis in Flow Cytometry Piedavent-Salomon M. | Sony Europe Company Presentati on: Partner – KRD 25. Across Living Colors Fluorescent Proteins and Cytometer Calibration Beads in Recent Science Lorencová M. 10.35 – 10.55
(8´)
(15´)
(8´)
Coffee Break
HALL A | EVROPA 1 | PARALLEL SESSION I 10.55 – 13.10
Cell Biology and Signalling Chairmen: Jaroš J., Kozubík A.
26. Variability in the Cellular Response to Activation of P53 on the Genome-Wide Scale Andrysik Z., Galbraith M.D., Allen M.A., Azofeifa J.G., Dowell R.D., Espinosa J.M. | Boulder, CO, USA
(20´)
27. Glycoprotein Asporin is Upregulated By Three-Dimensional (20´) Growth and Promotes Invasion of Triple-Negative Breast Cancer Cells Bouchal J., Simkova D., Kharaishvili G., Ozdian T., Soukup T., Krupka M., Galandakova A., Dzubak P., Mickova A., Korinkova G. | Olomouc, CZ 28. The Transcription Factor C/EBPγ is Dispensable for Hematopoiesis Kardošová M., Zjablovskaja P., Alberich-Jorda M. | Prague, CZ
(20´)
29. Valproic ACID Increases Cd133 Positive Cells that Show Stem Cell (20´) Features in Neuroblastoma Khalil M.A., Hraběta J., Groh T., Procházka P., Doktorová H., Eckschlager T. | Prague, CZ Company Presentati on: Gold Partner – BioTech 30. Think Antibody, Think Specific Think Imaging, Think Possible With Cytation Rego A., Fukalová J.
(15´)
31. Role of IFNalpha and IL-10 Dysregulation in Central Nervous System (20´) in Transgenic Porcine Huntington’s Disease Model Valekova I.1, 2, Jarkovska K.1, Kotrcova E.1, 2, Bucci J.3, Ellederova Z.1, Juhas S.1, Motlik J.1, Gadher S.J.4, Kovarova H.1 | 1Libechov, CZ, 2Prague, CZ, 3Carlsbad, CA, USA, 4Maryland, USA 32. New Challenges in the Treatment of Failing Heart: Focus (20´) on Inflammatory Responses of Innate Immune Cells Pekarová M.1, Ambrožová G.1, Martišková H.1, Koudelka A.1, Lojek A.1, Klinke A.2, Rudoplh T.K.2, Kadlec J.1, Trundova M.3, Kuchta R.1, Kuchtova Z.1, Kubala L. | 1Brno, CZ, 2Cologne, DE, 3Prague, CZ 12.50 – 14.15
Lunch / Lunch Sessions
HALL A | EVROPA 1 | EDUCATIONAL SESSION I 14.15 – 17.05
Image Analysis Chairman: Souček K., Ešner M.
33. Basics of Image Analysis Jaroš J. | Brno, CZ 34. Image Processing - Concepts and Methods Fedr R. | Brno, CZ 35. Optimization of Live Cell Imaging Ešner M. | Brno, CZ Discussion 15.55 – 16.15
Coffee Break
HALL A | | EVROPA 1 | CSAC MEMBERS ONLY 18.00 – 19.00
Plenary Meeti ng of the Czech Society for Analyti cal Cytology Chairmen: Kalina T., CSAC Committee
MONDAY, OCTOBER 5, 2015 HALL B | EVROPA 2 | PARALLEL SESSION II 10.55 – 12.50
Botany I Chairmen: Bureš P., Vrána J.
36. Flow Cytometric Detection of Holocentric Chromosomes in Plants Bureš P., Veselý P., Zedek F. | Brno, CZ
(20´)
37. Development of High-Lipid Content Strains of Microalgae Chlamydomonas Reinhardtii by Fluorescence-Activated Cell Sorting Búzová D., Fedorko J., Červený J. | Drásov, CZ
(20´)
Company Presentati on: Gold Partner – BioTech 38. Think Antibody, Think Specific Think Imaging, Think Possible With Cytation Rego A., Fukalová J. 39. Representative Amplification of DNA from Single Plant Chromosomes Cápal P., Blavet N., Vrána J., Kubaláková M., Doležel J. | Olomouc, CZ
(15´)
(20´)
40. 25-Fold Genome Size Difference in Genlisea – What Makes These (20´) Genomes Different? Tran T.D.1, Cao H.X.1, Jovtchev G.1, Neumann P.2, Novák P.2, Fojtová M.3, Vu G.T.H.1, Macas J.2, Fajkus J.3, Schubert I.1, Fuchs J.1 | 1Gatersleben, DE, 2 České Budějovice, CZ, 3Brno, CZ 41. Residual Sexuality in Supposed Apomicts: The Case of Sorbus Polyploids Lepší M.1, Koutecký P.1, Bílá J.2, Lepší P.3, Vít P.4 | 1České Budějovice, CZ, 2 Praha, CZ, 3Český Krumlov, CZ, 4Průhonice, CZ 12.50 – 14.15
Lunch / Lunch Sessions
14.15 – 15.55
Botany II Chairmen: Suda J., Šmarda P.
(20´)
42. Cell-Type-Specific Cytokinin Distribution Within the Arabidopsis (20´) Primary Root Apex Antoniadi I.1,2, Plačková L.3, Simonovik B.1, Doležal K.3, Turnbull C.2, Ljung K.1, Novák O.3 | 1Umeå, SE, 2London, UK, 3Olomouc, CZ 43. Flow Cytometry Teams Up With Mass Spectrometry to Unlock (20´) the Plant Nuclear Proteome Petrovská B., Jeřábková H., Chamrád I., Vrána J., Lenobel R., Uřinovská J., Šebela M., Doležel J. | Olomouc, CZ
44. Bionano Genome Mapping of Flow-Sorted Chromosomes (20´) Supports Physical Mapping and Sequence Assembly in Complex Plant Genomes Staňková H.1, Hastie A.2, Vrána J.1, Kubaláková M.1, Visendi P.3, Hayashi S.4, Luo M.C.5, Batley J.4, Edwards D.3, Doležel J.1, Šimková H.1 | 1Olomouc, CZ, 2 San Diego, USA, 3Brisbane, AU, 4Brisbane, AU, 5Davis, USA 45. Genomic GC Content Variation in Plants (20´) Šmarda P., Březinová A., Bureš P., Veselý P. | Brno, CZ 46. The Role of Whole-Genome Processes in the Origin (20´) of the Hyperdiverse Cape Flora Urfus T.1,2, Trávníček P.2, Bílá J.1, Vítová J.2, Chumová Z.1,Hanušová K.1, Suda J.1,2 | 1Prague, CZ, 2Průhonice, CZ 15.55 – 16.15
Coffee Break
MONDAY, OCTOBER 5, 2015 HALL C | MADRID 1 | EDUCATIONAL SESSION II | CZECH ONLY 14.15 – 17.05
Klinická cytometrie | CZ Předsedající: Vlková M., Kanderová V.
47. Kontrola kvality v klinické cytometrii aneb Jak se vyrovnat s ISO15189? Vlková M.1, Kanderová V.2 | 1Brno, CZ, 2Praha, CZ 48. Jak validovat a verifikovat cytometrická vyšetření? Vlková M.1, Kanderová V.2 | 1Brno, CZ, 2Praha, CZ Problémy, se kterými se v klinice potýkáme: 49. Titrace protilátek a kitů – co si můžeme dovolit? Vášková M. | Praha, CZ 50. Jak správně stanovit HLA-B27? Kryštůvková O. | Praha, CZ 51. CD8 Escapees aneb jak neztratit CD8 pozitivní T-lymfocyty? Vášková M., Kanderová V. | Praha, CZ 52. Co můžeme zjistit z vyšetření leukocytárních subpopulací v periferní krvi? Vlková M., Nechvátalová J. | Brno, CZ 53. Částečný IgD deficit Nechvátalová J., Chovancová Z. | Brno, CZ 15.55 – 16.15
Coffee Break
MONDAY, OCTOBER 5, 2015 HALL D | MADRID 2 | EDUCATIONAL SESSION III 14.15 – 17.05
FACS Sorti ng Chairman: Šinkora J., Vrána J.
54. Sorting vs. Analysis – Thinking Out of the Box (50´) Presentation about sorting, including tips,&tricks and extra panel design for sorting. Schlender J. | Erembodegem, BE 55. Sorting Out Sorting Issues What should the sorter operator know about your soting experiment. Semerák P., Kalina T. | Prague, CZ
(20´)
56. The BD FACSAria Fusion – Thinking In The Box Presentation focusing on Aria Fusion & Biosafety. Schlender J. | Erembodegem, BE
(20´)
57. What Can an Application Specialist Advice a FACSAria User? Experience form the field, configurations, Firmware tuning, standardization. Šinkora J. | Prague, CZ
(20´)
58. What We Are Proud of and What We Have Been Coping with (40´) Representatives of Individual Laboratories Will Be Requested to Prepare a Short Presentation About Their Top Applications and Successes. Their comments on bottlenecks and troubleshooting tips will be welcome. Sorter users 15.55 – 16.15
Coffee Break
TUESDAY, OCTOBER 6, 2015 HALL A | EVROPA 1 | PARALLEL SESSION III 8.30 – 10.30
Cytometry in Translati onal Research Chairmen: Fišer K.
59. Cell of Origin Search in Bladder Carcinoma: Cell Surface Phenotype (20´) and Chemosensitivity Analyzed by Multivariate Cytometry Drbal K., Müller N., Jariwala M-S., Matěj R., Stejskal J., Záleský M., Vicenová K., Zachoval R. | Prague, CZ 60. Expression and Relevance of Cancer Stem Cell Markers in Colorectal Cancer Matúšková M., Ďuriníková E., Kozovská Z., Kučerová L. | Bratislava, SK
(20´)
61. Analysis of Fluorescently Labeled Microvesicles and Exosomes Using (20´) Dedicated Flow Cytometry Pospichalova V., Svoboda J., Dave Z., Kotrbová A., Kaiser K., Klemova D., Ilkovics L., Hampl A., Crha I., Jandakova E., Minar L., Weinberger V., Bryja V. | Brno, CZ 62. Diagnostic Features Extracted from EuroFlow PID Dataset (20´) for Evaluation of Primary Immunodeficiency Stuchlý J.1, Kalina T.1, Šinkorová V.1, Mejstříková E.1, Nováková M.1, Vlková M.1, van Zelm M.C.3, Perez-Andres M.4, Lopez E.5, Orfao A.4, van Dongen J.J.M.3, van der Burg M.3 | 1Praha, CZ, 2Brno, CZ, 3Rotterdam, NL , 4Salamanca, ES, 5Madrid, Spain 63. Melanoma Regression in MeLiM Pigs Is Associated with Expansion (20´) of CD4+CD8+ T-Cells Sinkorova Z.1,2, Kucerova P.1, Cizkova J.1, Horak V.1, Planska D.3, Krizan J.1, Cervinkova M.1, Sinkora M.4, Sinkora J.4 | 1Liběchov, CZ, 2Hradec Králové, CZ, 3 Prague, CZ, 4Prague,CZ 64. EuroFlow Quality Assurance Program: Proposal for Structure (20´) and Implementation Kalina T.1, Flores-Montero J.2, Lecrevisse Q.2, Pedreira C.3, van der Velden V.H.J.4, Novakova M.1, Mejstrikova E.1, Hrusak O.1, Böttcher S.5, Karsch D.5, Sędek Ł.6, Trinquand A.7, Boeckx N.8, Caetano J.9, Asnafi V.7, Lucio P.9, Lima M.10, Santos A.H.10, Bonaccorso P.11, van der Sluijs-Gelling A.J.12, Langerak A.W.4, Martin-Ayuso M.2, Szczepański T.6, van Dongen J.J.M.4, Orfao A.2 | 1Prague, CZ, 2Salamanca, ES, 3 Rio de Janeiro, BR, 4Rotterdam, NL, 5Kiel, DE, 6Zabrze, PL, 7Paris, FR, 8Leuven, BE, 9 Lisbon, PT, 10Porto, PT, 11Monza, IT, 12The Hague, NL 10.30 – 10.50
Coffee Break
HALL A | EVROPA 1 | EDUCATIONAL SESSION IV 10.50 – 12.20
Cell Biology Chairman: Kalina T., Wallace P.K.
65. Introduction Wallace P.K. | Buffalo, NY, USA 66. Combined Detection of Surface and Intracellular Antigens: Opportunities and Obstacles Šimečková Š. | Brno, CZ 67. Fluorescent Barcoding in Facs-Based Profiling of Cell Surface Antigen Expressions Remšík J. | Brno, CZ 68. Phospho Flow in Immunodeficiency Kanderova V. | Prague, CZ 69. Multicolor Phenotyping With Proliferation Dyes and Viability Dyes Wallace P.K. | Buffalo, NY, USA 12.20 – 12.35
Closing Ceremony Chairmen: Kalina T., Doležel J.
12.35
Lunch
TUESDAY, OCTOBER 6, 2015 HALL B | EVROPA 2 | PARALLEL SESSION IV 8.30 – 10.30
Clinical Cytometry Chairperson: Vášková M.
70. Molecules Exhibiting Dipeptidyl Peptidase-LV-Like Enzymatic Activity in Local and Systemic Pathogenetic Processes of Rheumatoid Arthritis Sromova L., Busek P., Mareckova H, Sedova L., Sedo A. | Prague, CZ
(20´)
71. KV1.3 Lymphocyte Potassium Channel Inhibition as a Potential (20´) Novel Therapeutic Approach in Acute Ischemic Stroke Bajnok A., Folyovich A., Biró E., Orbán C., Vásárhelyi B., Toldi G. | Budapest, HU 72. Flow Cytometry In Bone Marrow Involvement with Mantle Cell (20´) Lymphoma – Correlations with Cytomorphology, Trephine Biopsy and Genetics Gyarfas J.1, Mlcakova A.1, Plank L.2, Szepe P.2, Balharek T.2, Marcinek J.2, Leitnerova M.1, Copakova L.1 | 1Bratislava, SK, 2Martin, SK 73. Different Immunological Characteristics of Cord Blood Cells (20´) of Newborns of Healthy and Allergic Mothers Hrdý J., Novotná O., Vlachynská K., Prokešová L. | Prague, CZ 74. B-Lymphocyte Maturation and T-Independent Response in Patients (20´) with Digeorge Syndrome Klocperk A., Kayserová J., Mejstříková E., Kalina T., Šedivá, A. | Prague, CZ 75. Phenotype Profile of Waldenström Macroglobulinaemia (20´) Rihova L.1, Vsianska P.1, Bezdekova R.1, Suska R.1, Stejskalova M.1, Adam Z.1, Penka M.1, Hajek R.1,2 | 1Brno, CZ, 2Ostrava, CZ 10.30 – 10.50
Coffee Break
HALL B | EVROPA 2 | EDUCATIONAL SESSION V | CZECH ONLY 10.50 – 12.20
Základy použití cytometrie | CZ Chairman: Janda J., Šinkora J.
76. Technické základy Svoboda J. | Praha, CZ 77. Fluorescence a kompenzace Janda J. | Praha, CZ 78. Standardizace Šinkora J. | Prague, CZ 79. Pravidla pro design vícebarevných panelů Vlček R. | Prague, CZ 12.35
Lunch
