LAMPIRAN A STANDARISASI SIMPLISIA HASIL PERHITUNGAN SUSUT PENGERINGAN SERBUK Replikasi
Hasil susut pengeringan daun belimbing manis 6,60% 6,80% 6,40%
Hasil susut pengeringan daun alpukat 5,30 % 5,20 % 5,10 %
1 2 3
5,30 % + 5,20 % + 5,10 % = 5,20% 3 6,60 % + 6,80 % + 6,40% Rata-rata daun belimbing manis : = 6,60% 3
Rata-rata daun alpukat :
HASIL PERHITUNGAN PENETAPAN KADAR ABU DAUN ALPUKAT No
W (krus kosong) (gram)
W (bahan) (gram)
W (krus + abu) (gram)
% Kadar Abu
Rerata
1 2 3
20,0074 20,0063 20,0090
2,0458 2,0438 2,0423
20,0885 20,0875 20,0897
7,17 7,20 7,23
7,22%
I. Kadar abu :
(berat kurs + serbuk) - berat kurs kosong X 100% berat serbuk :
II. Kadar abu :
20,0885− 20,0074 x 100 % = 7,17 % 2,0458
(berat kurs + serbuk) - berat kurs kosong X 100 % berat serbuk :
20,0875− 20,0063 x 100 % = 7,20 % 2,0438
84
(berat kurs + serbuk) - berat kurs kosong X 100 % berat serbuk
III Kadar abu :
:
Rata-rata kadar abu =
20,0897 − 20,0090 x 100 % = 7,23 % 2,0423 7,17 % + 7,20 % + 7,23 % = 3,96 % 3
HASIL PERHITUNGAN PENETAPAN KADAR ABU DAUN BELIMBING MANIS No
W (krus kosong) (gram)
W (bahan) (gram)
W (krus + abu) (gram)
% Kadar Abu
Rerata
1 2 3
20,0074 20,0063 20,0070
2,0458 2,0438 2,0040
20,0885 20,0875 20,1507
7,23 7,20 7,17
7,22 %
I. Kadar abu :
(berat kurs + serbuk) - berat kurs kosong X 100 % berat serbuk :
II. Kadar abu :
20,1525− 20,0074 x 100 % = 7,23 % 2,0060
(berat kurs + serbuk) - berat kurs kosong X 100 % berat serbuk :
20,1522 − 20,0081 x 100 % = 7,20 % 2,0022 (berat kurs + serbuk) - berat kurs kosong X 100 % berat serbuk
III Kadar abu :
:
20,1507 − 20,0070 x 100 % = 7,17 % 2,0040
Rata-rata kadar abu =
7,23% + 7,20 % + 7,17 % = 7,22 % 3 85
HASIL PERHITUNGAN RANDEMEN EKSTRAK Daun alpukat Daun belimbing manis
berat ekstrak kental X 100% berat serbuk berat ekstrak kental X 100% berat serbuk 187,2236 x 100 %= 62,40 % 300,0043
119,2521 x 100 %= 39,74 % 300,0058 HASIL PERHITUNGAN KADAR SARI LARUT ETANOL DAUN ALPUKAT Berat cawan Berat cawan Berat ekstrak No +ekstrak setelah kosong diuapkan 1 69,1472 68,4391 5,0033 2
69,5139
68,5683
5,0098
3
68,3580
67,5876
5,1311
I Kadar sari larut etanol =
(berat cawan + ekstrak) - (berat cawan kosong) X 100% berat ekstrak =
69,1472 − 68,4391 x 100 % = 14,15 % 5,0033
II Kadar sari larut etanol =
(berat cawan + ekstrak) - (berat cawan kosong) X 100% berat ekstrak
86
=
69,5139 − 68,683 x 100 % = 18,88 % 5,0018
III Kadar sari larut etanol =
(beratcawan+ ekstrak)- (beratcawankosong) X100% beratekstrak =
68,3580 − 67,5876 x 100 % = 15,01 % 5,1311
Rata-rata kadar sari larut etanol =
14,15% + 18,88% + 15,01% = 16,01 % 3
HASIL PERHITUNGAN KADAR SARI LARUT ETANOL BELIMBING MANIS Berat cawan Berat cawan Berat ekstrak No +ekstrak setelah kosong diuapkan 1 67,7910 66,9725 5,2366 2 67,9444 67,1617 5,1326 3 70,0808 69,2513 5,2502 I Kadar sari larut etanol =
(berat cawan + ekstrak) - (berat cawan kosong) X 100% berat ekstrak =
67,7910 − 66,9725 x 100 % = 15,63 % 5,2366
II Kadar sari larut etanol =
(berat cawan + ekstrak) - (berat cawan kosong) X 100% berat ekstrak =
67,9444 − 67,1617 x 100 % = 15,25% 5,1326
87
III Kadar sari larut etanol =
(berat cawan + ekstrak) - (berat cawan kosong) X 100% berat ekstrak =
70,0808 − 69,2513 x 100 % = 15,80 % 5,2502
Rata-rata kadar sari larut etanol =
15,63% + 15,25% + 15,80% = 15,56 3
88
LAMPIRAN B RANGKUMAN RUMUS ANAVA
Rumus anava rambang lugas digunakan untuk beda antar perlakuan yang lebih dari 2, dimana tiap kelompok dihitung dengan harga n, x, ∑x, ∑x2, kemudian dihitung nilai= N = jumlah subyek seluruhnya = n1+n2+ n3+ n4+n5 J = Ji = Jumlah data total = ∑x1+∑x2+∑x3+∑x4+∑x5 ∑Y2ij = Jumlah kuadrat data total = ∑x12+∑x22+∑x32+∑x42+∑x52 J2i = jumlah kuadrat dari J = Ji = (∑x1+∑x2+∑x3+∑x4+∑x5)2 JKT = jumlah kuadrat total = ∑Y2ij - ∑J2 / N JKPy = jumlah kuadrat perlakuan = ∑J2i - ∑J2 / N JKEy = jumlah kuadrat dalam = JKT- JKPy dbT = derajat bebas total = N – 1 dbPy = derajat bebas perlakuan = a – 1 dbEy = derajat bebas dalam = dbT-dbPy RJKPy = rataan jumlah kuadrat perlakuan = JKPy/dbPy RJKEy = rataan jumlah kuadrat dalam = JKEy/ dbEy Fhitung = RJKPy/RJKEy Ket : N = jumlah subyek dalam perlakuan a = jumlah perlakuan Dari hasil pengolahan data tersebut akan diperolah harga F hitung yang kemudian akan dibandingkan dengan harga F tabel. Kriteria pengujian : • Bila F hitung < F tabel , maka terdapat perbedaan yang tidak bermakna • Bila F hitung > Ftabel, meka terdapat perbedaan yang bermakna Untuk mengetahui adanya perbedaan efek perlakuan antar pasangan kelompok perlakuan terhadap kadar glukosa darah, maka dilakukan uji HSD 5 % dan 1 % HSD 5 % =
q (0,05; P, db / dk ) 2
HSD 1 % = q (0,01; P, db / dk )
2
89
1 ⎞ ⎛ 1 RJK ⎜ + ⎟ ⎝ nA nB ⎠ 1 ⎞ ⎛ 1 RJK ⎜ + ⎟ nA nB ⎝ ⎠
LAMPIRAN C
5 5 5 5 5 25
70.8000 70.0000 71.4000 69.8000 69.6000 70.3200
Mean 2.77489 7.00000 6.80441 5.80517 1.14018 4.84527 5.25357
Std. Deviation 67.3545 61.3084 62.9512 62.5919 68.1843 68.3200 68.1282 67.4028a
1.05071a
Lower Bound
1.24097 3.13050 3.04302 2.59615 .50990 .96905 1.05071
Std. Error
73.2372a
74.2455 78.6916 79.8488 77.0081 71.0157 72.3200 72.5118
68.00 61.00 64.00 63.00 68.00 61.00
75.00 78.00 79.00 76.00 71.00 79.00
Upper Bound Minimum Maximum
90
a. Warning: Between-component variance is negative. It was replaced by 0.0 in computing this random effects measure.
Random Effects
kontrol t0 75 : 25 t0 50 : 50 t0 25 : 75 t0 pembanding t0 Total Model Fixed Effects
N
95% Confidence Interval for Mean
Descriptives
Perhitungan Anava Kadar Glukosa Darah Puasa Tikus Putih (0 menit)
PERHITUNGAN ANAVA KADAR GLUKOSA DARAH PUASA TIKUS PUTIH PADA BERBAGAI WAKTU PENGAMATAN
552.000 563.440
Within Groups Total
24
20
4
df
27.600
2.860
Mean Square .104
F .980
Sig.
Dari hasil anova one way menggunakan bantuan komputer program SPSS 16.0 diatas dapat diketahui F hitung <
11.440
Between Groups
Squares
Sum of
ANOVA
91
perlakuan dengan kelompok control dan distribusi sampel homogen.
F tabel, maka Ho diterima dan Ha ditolak, dengan demikan tidak terdapat perbedaan bermakna antar kelompok
5 5 5 25
50 : 50 t30
25 : 75 t30
pembanding t30
Total
Effects
Random
Fixed Effects
5
75 : 25 t30
Model
5
N
kontrol t30
for Mean
3.11448
9.93982
7.29383
8.10555
8.73499
7.79359
92
5.51957
1.55872
2.66501
1.39284
4.44522
3.26190
3.62491
3.90640
96.5152
108.5886
106.3397
86.3329
104.2581
106.1435
105.7356
110.5541
127.1648
115.0914
117.3403
94.0671
128.9419
124.2565
125.8644
132.2459
87.00
87.00
101.00
107.00
106.00
108.00
132.00
95.00
127.00
123.00
124.00
132.00
Deviation Std. Error Lower Bound Upper Bound Minimum Maximum
111.8400 13.32504
90.2000
116.6000
115.2000
115.8000
121.4000
Mean
Std.
95% Confidence Interval
Perhitungan Anava Kadar Glukosa Darah Tikus Putih (30 menit) Descriptives
3046.560 1214.800 4261.360
Between Groups Within Groups Total
Sum of Squares
24
20
4
df
93
ANOVA
60.740
761.640
Mean Square 12.539
F .000
Sig.
Effects
5 5 5 5 5 25
N 111.0000 90.8000 94.4000 102.2000 79.4000 95.5600
Mean 9.79796 6.68581 18.43366 9.44458 3.91152 14.68866 10.81665 80.7687
5.32744
94
98.8342 82.4985 71.5116 90.4730 74.5432 89.4968 91.0474
Lower Bound
4.38178 2.98998 8.24379 4.22374 1.74929 2.93773 2.16333
Std. Deviation Std. Error
110.3513
123.1658 99.1015 117.2884 113.9270 84.2568 101.6232 100.0726
Upper Bound
95% Confidence Interval for Mean
Perhitungan Anava Kadar Glukosa Darah Tikus Putih (60 menit) Descriptives
kontrol t60 75 : 25 t60 50 : 50 t60 25 : 75 t60 pembanding t60 Total Mode Fixed Effects l Random
101.00 81.00 68.00 92.00 75.00 68.00
Minimum
125.00 98.00 113.00 114.00 84.00 125.00
Maximum
2838.160 2340.000 5178.160
Between Groups Within Groups Total
Sum of Squares
95
24
20
4
df
ANOVA
117.000
709.540
Mean Square 6.064
F .002
Sig.
5 86.0000 5 67.2000 25 84.2400 13.61151
25 : 75 t120
pembanding t120
Total
Model Fixed Effects
5 87.8000 17.21046
50 : 50 t120
9.61769
2.77489
7.10634
9.88939
5 81.6000
3.20936
Deviation
75 : 25 t120
Mean 5 98.6000
N
Std.
96
1.92354
2.72230
1.24097
3.17805
7.69675
4.42267
1.43527
Error
Std.
80.2276
78.6214
63.7545
77.1763
66.4304
69.3207
94.6151
88.2524
89.8586
70.6455
94.8237
109.1696
93.8793
102.5849
Lower Bound Upper Bound
Mean
95% Confidence Interval for
Perhitungan Anava Kadar Glukosa Darah Tikus Putih (120 menit) Descriptives
kontrol t120
64.00
64.00
80.00
64.00
67.00
94.00
m
Minimu
108.00
71.00
96.00
108.00
93.00
102.00
Maximum
2596.560 1850.000 4446.560
Between Groups Within Groups Total
Sum of Squares
24
20
4
df
97
ANOVA
92.500
649.140
Mean Square 7.018
F .001
Sig.
25
Total
2.16795
.96954
3.01662
75.6000 13.12123 2.62425
60.8000
6.74537
Effects
98
4.16989
5
pembanding t180
80.0000
80.0000 17.88854 8.00000
Random
5
25 : 75 t180
3.57211
10.93892 2.18778
5
50 : 50 t180
7.98749
Error
72.6000 12.81796 5.73236
84.6000
Mean Deviation
Std.
Fixed Effects
5
75 : 25 t180
Model
5
N
Std.
64.0225
71.0364
70.1838
58.1081
71.6245
57.7884
56.6844
74.6822
87.1775
80.1636
81.0162
63.4919
88.3755
102.2116
88.5156
94.5178
51.00
58.00
76.00
54.00
51.00
74.00
Lower Bound Upper Bound Minimum
for Mean
95% Confidence Interval
Perhitungan Anava Kadar Glukosa Darah Tikus Putih (180 menit) Descriptives
kontrol t180
102.00
63.00
92.00
102.00
84.00
94.00
Maximum
2393.200
Within Groups
20
4
df
119.660
434.700
Mean Square 3.633
F .022
Sig.
Total 4132.000 24 Dari hasil anova one way menggunakan bantuan komputer program SPSS 17.0 diatas dapat diketahui F hitung >
1738.800
Between Groups
Sum of Squares
ANOVA
ŬĞůŽŵƉŽŬ͘
99
dengan kelompok kontrol, maka dilanjutkan dengan uji HSD 5 % untuk melihat di mana letak perbedaan bermakna tiap
F tabel, maka Ho ditolak dan Ha diterima, dengan demikian ada perbedaan bermakna antar kelompok perlakuan
31.20000*
75 : 25 t30
4.92910
4.80000
25 : 75 t30
100
4.92910
25.60000*
pembanding t30
4.92910
-.80000
25 : 75 t30
4.92910
.60000
50 : 50 t30
4.92910
-5.60000
4.92910
kontrol t30
pembanding t30
4.92910
6.20000
50 : 50 t30
4.92910
5.60000
75 : 25 t30
kontrol t30
Difference (I-J) Std. Error
(J) perlakuan
Mean
.000
1.000
1.000
.786
.000
.864
.719
.786
Sig.
Perhitungan uji HSD pada menit ke 30 Multiple Comparisons
10.8503
-15.5497
-14.1497
-20.3497
16.4503
-9.9497
-8.5497
-9.1497
Lower Bound
40.3497
13.9497
15.3497
9.1497
45.9497
19.5497
20.9497
20.3497
Upper Bound
95% Confidence Interval
PERHITUNGAN UJI HSD PADA BERBAGAI WAKTU PENGAMATAN
LAMPIRAN D
(I) perlakuan
4.92910 4.92910
-1.40000 25.00000*
25 : 75 t30 pembanding t30
4.92910 4.92910 4.92910 4.92910
-31.20000* -25.60000* -25.00000* -26.40000*
kontrol t30 75 : 25 t30 50 : 50 t30 25 : 75 t30
101
4.92910
26.40000*
pembanding t30
4.92910
1.40000
50 : 50 t30
4.92910
.80000
75 : 25 t30
4.92910
-4.80000
kontrol t30
4.92910
-.60000
75 : 25 t30
4.92910
-6.20000
kontrol t30
*. The mean difference is significant at the 0.05 level.
pembanding t30
25 : 75 t30
50 : 50 t30
.000
.001
.000
.000
.000
.998
1.000
.864
.001
.998
1.000
.719
-41.1497
-39.7497
-40.3497
-45.9497
11.6503
-13.3497
-13.9497
-19.5497
10.2503
-16.1497
-15.3497
-20.9497
-11.6503
-10.2503
-10.8503
-16.4503
41.1497
16.1497
15.5497
9.9497
39.7497
13.3497
14.1497
8.5497
31.60000*
50 : 50 t60
75 : 25 t60
6.84105
8.80000
25 : 75 t60
102
3.60000
11.40000
pembanding t60
75 : 25 t60
-11.40000
25 : 75 t60
-16.60000
-3.60000
50 : 50 t60
kontrol t60
-20.20000
kontrol t60
pembanding t60
6.84105
16.60000
50 : 50 t60
6.84105
6.84105
6.84105
6.84105
6.84105
6.84105
6.84105
6.84105
20.20000
75 : 25 t60
kontrol t60
Difference (I-J) Std. Error
(J) perlakuan
Mean
.984
.149
.475
.475
.984
.054
.001
.702
.149
.054
Sig.
Perhitungan uji HSD pada menit ke 60 Multiple Comparisons
(I) perlakuan
-16.8710
-37.0710
-9.0710
-31.8710
-24.0710
-40.6710
11.1290
-11.6710
-3.8710
-.2710
Lower Bound
24.0710
3.8710
31.8710
9.0710
16.8710
.2710
52.0710
29.2710
37.0710
40.6710
Upper Bound
95% Confidence Interval
-22.80000
-15.00000
50 : 50 t60 25 : 75 t60
-11.40000
75 : 25 t60
103
*
-31.60000
*
kontrol t60
22.80000
7.80000
50 : 50 t60 pembanding t60
11.40000
75 : 25 t60
*
-8.80000
15.00000
pembanding t60 kontrol t60
-7.80000
25 : 75 t60
*. The mean difference is significant at the 0.05 level.
pembanding t60
25 : 75 t60
6.84105
6.84105
6.84105
6.84105
6.84105
6.84105
6.84105
6.84105
6.84105
6.84105
.024
.223
.475
.001
.024
.784
.475
.702
.223
.784
-43.2710
-35.4710
-31.8710
-52.0710
2.3290
-12.6710
-9.0710
-29.2710
-5.4710
-28.2710
-2.3290
5.4710
9.0710
-11.1290
43.2710
28.2710
31.8710
11.6710
35.4710
12.6710
-4.40000 14.40000*
25 : 75 t120 pembanding t120
50 : 50 t120
104
6.20000
6.08276
-6.20000
50 : 50 t120
75 : 25 t120
6.08276
-17.00000*
kontrol t120
-10.80000
6.08276
31.40000*
pembanding t120
kontrol t120
6.08276
12.60000
25 : 75 t120
75 : 25 t120
6.08276
10.80000
50 : 50 t120
6.08276
6.08276
6.08276
6.08276
6.08276
17.00000*
75 : 25 t120
Std. Error
kontrol t120
(I-J)
(J) perlakuan
Mean Difference
.320
.091
.028
.478
.320
.011
.000
.051
.091
.011
Sig.
Perhitungan uji HSD pada menit ke 120 Multiple Comparisons
(I) perlakuan
-6.4884
-23.4884
1.7116
-17.0884
-18.8884
-29.6884
18.7116
-.0884
-1.8884
4.3116
Lower Bound
18.8884
1.8884
27.0884
8.2884
6.4884
-4.3116
44.0884
25.2884
23.4884
29.6884
Upper Bound
95% Confidence Interval
-14.40000 -20.60000 -18.80000
75 : 25 t120 50 : 50 t120 25 : 75 t120
*
*
*
*
105
-31.40000
kontrol t120
18.80000
-1.80000
50 : 50 t120 pembanding t120
4.40000
75 : 25 t120
*
-12.60000
20.60000
*
1.80000
kontrol t120
pembanding t120
25 : 75 t120
*. The mean difference is significant at the 0.05 level.
pembanding t120
25 : 75 t120
6.08276
6.08276
6.08276
6.08276
6.08276
6.08276
6.08276
6.08276
6.08276
6.08276
.006
.003
.028
.000
.006
.770
.478
.051
.003
.770
-31.4884
-33.2884
-27.0884
-44.0884
6.1116
-14.4884
-8.2884
-25.2884
7.9116
-10.8884
-6.1116
-7.9116
-1.7116
-18.7116
31.4884
10.8884
17.0884
.0884
33.2884
14.4884
23.80000*
50 : 50 t180
75 : 25 t180
6.91838
4.60000
25 : 75 t180
.00000
25 : 75 t180
106
7.40000
11.80000
pembanding t180
75 : 25 t180
-7.40000
25 : 75 t180
-4.60000
-7.40000
50 : 50 t180
kontrol t180
-12.00000
kontrol t180
pembanding t180
6.91838
4.60000
50 : 50 t180
6.91838
6.91838
6.91838
6.91838
6.91838
6.91838
6.91838
6.91838
6.91838
12.00000
75 : 25 t180
Std. Error
kontrol t180
(I-J)
(J) perlakuan
Mean Difference
1.000
.820
.962
.453
.820
.820
.437
.019
.962
.962
.437
Sig.
Perhitungan uji HSD pada menit ke 180 Multiple Comparisons
(I) perlakuan
-20.7024
-13.3024
-25.3024
-8.9024
-28.1024
-28.1024
-32.7024
3.0976
-16.1024
-16.1024
-8.7024
Lower Bound
20.7024
28.1024
16.1024
32.5024
13.3024
13.3024
8.7024
44.5024
25.3024
25.3024
32.7024
Upper Bound
95% Confidence Interval
-19.20000 -19.20000
50 : 50 t180 25 : 75 t180
107
-11.80000
75 : 25 t180
6.91838
6.91838
6.91838
6.91838
-23.80000*
kontrol t180
6.91838 6.91838
.00000
50 : 50 t180
6.91838
6.91838
6.91838
19.20000
7.40000
75 : 25 t180
pembanding t180
-4.60000
19.20000
kontrol t180
pembanding t180
*. The mean difference is significant at the 0.05 level.
pembanding t180
25 : 75 t180
.077
.077
.453
.019
.077
1.000
.820
.962
.077
-39.9024
-39.9024
-32.5024
-44.5024
-1.5024
-20.7024
-13.3024
-25.3024
-1.5024
1.5024
1.5024
8.9024
-3.0976
39.9024
20.7024
28.1024
16.1024
39.9024
LAMPIRAN E TABEL DERAJAT KEBEBASAN
108
LAMPIRAN F TABEL UJI HSD (0,05)
Sumber : Schefler (1987) 109
LAMPIRAN G
110
LAMPIRAN H TABEL KORELASI (r)
Sumber : Soedigdo S., Soedigdo P., 1977
111
LAMPIRAN I SURAT DETERMINASI TANAMAN BELIMBING MANIS
112
LAMPIRAN J SURAT DETERMINASI TANAMAN ALPUKAT
LAMPIRAN K 113
SERTIFIKAT GLIBENCLAMIDE
114
LAMPIRAN L DATA QUALITY CONTROL Nilai yang tertera pada tabel standart serum control untuk glucose adalah 82,8-1414 mg/dL(590009983061) Data ke-
Hasil
Interpretasi
1
97
Memenuhi Syarat
2
98
Memenuhi Syarat
3
97
Memenuhi Syarat
Rata-rata
97,33
Memenuhi Syarat
SD
97±0,47
Memenuhi Syarat
115
