LAMPIRAN 1 HASIL DETERMINASI DAUN ANGSANA
86
LAMPIRAN 2 HASIL PERHITUNGAN
Hasil Perhitungan Randemen Ekstrak
•
ܴ݁݊݀݁݉݁݊ =
•
ሺberat cawan + ekstrak kentalሻ − berat cawan kosong x 100% berat ekstrak cair ሺ572,8 + 57,3ሻ − 572,8 = x 100% = 48,6% 117,9 Hasil skrinning fitokimia No.
•
1.
Analisis Alkaloid
Hasil Analisis +
2.
Flavonoid
+
3.
Tanin
+
4.
Saponin
-
5.
Kuinon
-
6.
Sterol/Terpen
+ (terpen)
Keterangan Terbentuk endapan putih dan jingga Lapisan amil alcohol (berwarna kuning) terpisah dengan alkohol klorhidrik Terbentuk warna hijau Tidak tebentuk busa yang stabil Tidak terbentuk endapan merah Terbentuk warna hijau
Hasil Perhitungan Kadar Air pada Ekstrak Kental I.
Kadar Air = =
II.
Kadar Air = =
III.
Kadar Air =
௩௨ ௬ ௧ ௧ ௦௦
,ସ଼ସ ହ,ଷ
ݔ100% = 9,6%
௩௨ ௬ ௧ ௧ ௦௦
,ସ଼ ହ,ଵଷ
ݔ100%
ݔ100%
ݔ100% = 9,31%
௩௨ ௬ ௧ ௧ ௦௦
ݔ100% 87
= Rata-rata kadar air =
•
,ସ଼ଷ ହ,ଵ
ݔ100% = 9,47%
ଽ,ାଽ,ଷଵାଽ,ସ ଷ
= 9,46%
Hasil Perhitungan Harga Rf pada pemeriksaan KLT dengan pelarut= n-butanol : asam asetat glasial : air (4 : 1 : 5) 254 nm Ekstrak 0,24 0,31 0,6
366 nm Ekstrak Rutin 0,25 0,37 0,56 0,6
Rutin
0,6
Contoh perhitungan harga Rf : Rf Ekstrak etanol angsana = Rf Rutin =
ସ,ହ ଼
ସ,ହ ଼
= 0,56
= 0,56
88
LAMPIRAN 3 UJI ENZIMATIS •
Tabel Pengolahan Data % Inhibisi Acarbose
Konsentrasi Acarbose (µg/ml)
Aktivitas Enzim α-glucosidase tanpa Inhibitor
Kontrol
0,00019 0,00020 0,00018 0,00019 0,00020 0,00018 0,00019 0,00020 0,00018 0,00019 0,00020 0,00018 0,00019 0,00020 0,00018 0,00019 0,00020 0,00018
50
250
1000
2000
4000
Aktivitas Enzim α-glucosidase dengan Inhibitor
0,00016 0,00015 0,00013 0,00007 0,00007 0,00007 0,00004 0,00005 0,00005 0,00004 0,00003 0,00004 0,00003 0,00004 0,00003
% Inhibisi
% Inhibisi rata-rata
16,262 21,154 25,199 63,350 64,904 64,191 79,612 74,760 70,292 79,612 82,933 79,045 86,408 78,846 85,146
20,872
64,148
74,888
80,530
83,467
Contoh perhitungan aktivitas acarbose 50 µg/ml: % Inhibisi = % Inhibisi =
aktivitas enzim tanpa inhibisi − aktivitas enzim dengan inhibisi × 100% aktivitas enzim tanpa inhibisi ,ଵଽି,ଵ ,ଵଽ
× 100%
= 16,262 %
89
•
Tabel Pengolahan Data % Inhibisi Ekstrak Etanol Daun Angsana
Konsentrasi Acarbose (µg/ml) Kontrol
50
250
Aktivitas Enzim α-glucosidase tanpa Inhibitor 0,00031 0,00033 0,00030 0,00031 0,00033 0,00030 0,00031 0,00033 0,00030
Aktivitas Enzim α-glucosidase dengan Inhibitor
% Inhibisi
0,00027 0,00027 0,00026 0,00019 0,00021 0,00020
12,903 18,182 13,333 38,710 36,364 33,333
14,806
0,00031
0,00018
41,935
41,453
0,00033 0,00030 0,00031 0,00033 0,00030 0,00031 0,00033 0,00030
0,00019 0,00018 0,00015 0,00016 0,00014 0,00013 0,00013 0,00012
42,424 40,000 51,613 51,515 53,333 58,065 60,606 60,000
1000
2000
4000
% Inhibisi rata-rata
36,136
52,154
59,557
Contoh perhitungan aktivitas ekstrak etanol daun angsana 50 µg/ml: % Inhibisi = % Inhibisi =
aktivitas enzim tanpa inhibisi − aktivitas enzim dengan inhibisi × 100% aktivitas enzim tanpa inhibisi ,ଷଵି,ଶ ,ଷଵ
× 100%
= 16,129 %
90
Tabel Pengolahan Data Kinetika Enzim Tanpa Inhibitor
•
Replikasi
Persamaan Regresi
1
Y= 73,51692 + 72,06785x Y= 69,14244 + 80,35815x Y = 73,08701 + 76,2741x
2 3
(-ൗࡷ ሻ ࡹ -1,02011
ൗ ࢂࢇ࢞ 73,51692
ࡷࡹ
ࢂࢇ࢞
0,980289
0,013602
-0,86043
69,14244
1,162212
0,014463
-0,95822
73,08701
1,043607
0,013682
1,062036 ± 0,075
0,013916 ± 0,00038
Rata-rata ± SD
Contoh perhitungan: Perhitungan K ቀy = 0 ; x = −
ଵ
ಾ
ቁ
Perhitungan ܸ௫ ቀx = 0 ; y =
ܽ = ݕ+ ܾݔ
y = 73,51692 + 72,06785x
0 = 73,51692 + 72,06785ቀ− ଵ
ಾ
ቁ
ܽ = ݕ+ ܾݔ
y = 73,51692 + 72,06785x
ቀ−
ଵ
ೌೣ
ଵ
ಾ
ቁ
ቀ
ቁ = -1,02011
ଵ
ೌೣ
ቁ = 73,51692 + 72,06785
(0) ቀ
ܭெ = 0,980289
ଵ
ೌೣ
ቁ = 73,51692
ܸெ௫ = 0,013602
Tabel Pengolahan Data Kinetika Enzim dengan Inhibitor
•
Ekstrak Etanol daun Angsana 100 µg/ml Replikasi
Persamaan Regresi
1
Y = 63,574 + 76,962x Y = 48,9953 + 92,40x Y = 59,563 + 81,135x
2 3
(-ൗࡷ ሻ ࡹ -0,82604
ൗ ࢂࢇ࢞ 63,574
ࡷࡹ
ࢂࢇ࢞
1,21059
0,01573
-0,53025
48,9953
1,88589
0,02041
-0,73412
59,563
1,36217
0,01679
1,48622±0,29
0,01764±0,002
Rata-rata ± SD
Contoh perhitungan: Perhitungan K ቀy = 0 ; x = −
ଵ
ಾ
ቁ
ܽ = ݕ+ ܾݔ
ቀ−
ଵ
ಾ
ቁ = -0,82604
ܭெ = 1,21059
ଵ
ೌೣ
ቁ
ܽ = ݕ+ ܾݔ
y = 63,574 + 76,962x 0 = 63,574 + 76,962ቀ−
Perhitungan ܸ௫ ቀx = 0 ; y =
y = 63,574 + 76,962x ଵ
ಾ
ቁ
ቀ ቀ
ଵ
ቁ = 63,574 + 76,962 (0)
ଵ
ቁ = 63,574
ೌೣ ೌೣ
ܸெ௫ = 0,01573
91
Tabel Pengolahan Data Kinetika Enzim dengan Inhibitor
•
Ekstrak Etanol daun Angsana 200 µg/ml Replikasi
Persamaan Regresi Y = 63,3838 +106,4722x Y = 70,30083 + 95,75449 Y = 56,36422 + 125,8381
1 2 3
(-ൗࡷ ሻ ࡹ
ൗ ࢂࢇ࢞
ࡷࡹ
ࢂࢇ࢞
-0,59531
63,38383
1,679801
0,015777
-0,73418
70,30083
1,362068
0,014225
-0,44791
56,36422
2,232588
0,017742
1,758152±0,36
0,015914±0,016
Rata-rata ± SD
Contoh perhitungan: Perhitungan K ቀy = 0 ; x = −
ଵ
ಾ
ቁ
Perhitungan ܸ௫ ቀx = 0 ; y =
ܽ = ݕ+ ܾݔ
y = 63,3838 +106,4722x
0 = 63,3838 +106,4722ቀ− ଵ
ಾ
ቁ
ܽ = ݕ+ ܾݔ
y = 63,3838 +106,4722x
ቀ−
ଵ
ೌೣ
ଵ
ಾ
ቁ
ቀ
ቁ = -0,59531
ቀ
ܭெ = 1,679801
ଵ
ቁ = 63,3838 +106,4722 (0)
ଵ
ቁ = 63,38383
ೌೣ ೌೣ
ܸெ௫ = 0,015777
Tabel Pengolahan Data Kinetika Enzim dengan Inhibitor
•
Ekstrak Etanol daun Angsana 500 µg/ml Replikasi
Persamaan Regresi
1
Y=69,89402+160,9472x
2
Y=71,57646+137,4988x
3
Y=55,7469 + 165,0762x
(ൗ ሻ ൗࢂࢇ࢞ ࡷࡹ 69,89402 0,4343 71,57646 0,5205 55,7469 0,3377 Rata-rata ± SD
ࡷࡹ
ࢂࢇ࢞
2,302732
0,014307
1,921006
0,013971
2,961173
0,017938
2,39497±0,42
0,015406±0,018
Contoh perhitungan: Perhitungan K ቀy = 0 ; x = −
ଵ
ಾ
ቁ
Perhitungan ܸ௫ ቀx = 0 ; y =
ܽ = ݕ+ ܾݔ
ቀ−
ଵ
ಾ
ቁ = -0,4343
ܭெ = 2,302732
ቁ
ܽ = ݕ+ ܾݔ
y = 69,89402+160,9472x 0 = 69,89402+160,9472 ቀ−
ଵ
ೌೣ
y = 69,89402+160,9472x ଵ
ಾ
ቁ
ቀ ቀ
ଵ
ቁ = 69,89402+160,9472 (0)
ଵ
ቁ = 69,89402
ೌೣ ೌೣ
ܸெ௫ = 0,014307 92
LAMPIRAN 4 PRINTOUT ANALISA STATISTIK •
Printout Analisa Statistik Nilai IC50
T-TEST GROUPS=kelompok(1 2) /MISSING=ANALYSIS /VARIABLES=IC50 /CRITERIA=CI(.95). T-Test Notes Output Created Comments Input
Missing Value Handling
Syntax
Resources
16-Nov-2014 18:25:18 Data
C:\Users\Erica\Desktop\Hasil skripsi\finish\SPSS\Independe nt sample T test\perbandingan IC50 acarbose dgn ekstrak.sav Active Dataset DataSet1 Filter <none> Weight <none> Split File <none> N of Rows in Working 6 Data File Definition of Missing User defined missing values are treated as missing. Cases Used Statistics for each analysis are based on the cases with no missing or out-of-range data for any variable in the analysis. T-TEST GROUPS=kelompok(1 2) /MISSING=ANALYSIS /VARIABLES=IC50 /CRITERIA=CI(.95). Processor Time 0:00:00.015 Elapsed Time
0:00:00.013
Group Statistics
93
kelompok N IC5 Acarbose
Mean
Std. Deviation
Std. Error Mean
3 216.01166666666 19.0539718781501 11.0008157929
0
7 ekstrak
815
3 1743.9376666666 102.453980753962 59.1518333678
etanol
67
5
490
Independent Samples Test Leve ne's Test for Equal ity of Varia nces
t-test for Equality of Means 95% Confidence Interval of the Difference
Si F g.
t
Sig . (2tail Mean Std. Error df ed) Difference Difference
Lower
Upper
IC Equa 2. .1 - 4 .00 - 6.0166081 50 l 97 6 25.3 0 1.5279260 299091E1 1.6949738 1.3608781 varia 5 0 95 000000E3 219218E3 780782E3 nces assu med Equa l varia nces not assu med
- 2. .00 - 6.0166081 25.3 13 1 1.5279260 299091E1 1.7714102 1.2844417 95 8 000000E3 542965E3 457035E3
94
•
Printout Analisa Statistik Nilai ࢂࢇ࢞
Oneway Notes Output Created Comments Input
Missing Value Handling
Syntax
Resources
11-Nov-2014 12:11:55 Data
C:\Users\Erwin\Desktop\Hasil skripsi\finish\SPSS\oneway ANOVA\KM VM oneway ANOVA.sav DataSet1 <none> <none> <none> 12
Active Dataset Filter Weight Split File N of Rows in Working Data File Definition of Missing User-defined missing values are treated as missing. Cases Used Statistics for each analysis are based on cases with no missing data for any variable in the analysis. ONEWAY Vmax BY kelompok /STATISTICS HOMOGENEITY /MISSING ANALYSIS. Processor Time 0:00:00.000 Elapsed Time
0:00:00.000
95
Test of Homogeneity of Variances Vmax Levene Statistic
df1
2.223
df2 3
Sig. 8
.163
ANOVA Vmax Sum of Squares Between
df
Mean Square
.000
3
.000
Within Groups
.000
8
.000
Total
.000
11
F 1.998
Sig. .193
Groups
96
LAMPIRAN 5 PENENTUAN PANJANG GELOMBANG MAKSIMAL
97
LAMPIRAN 6 TABEL T
98
