LAMPIRAN A SURAT DETERMINASI TANAMAN PUTRI MALU

LAMPIRAN A SURAT DETERMINASI TANAMAN PUTRI MALU

69

LAMPIRAN B SERTIFIKAT HEWAN COBA

70

LAMPIRAN C SERTIFIKAT KODE ETIK

71

LAMPIRAN D DASAR PENGGUNAAN DOSIS Dalam penelitian ini penggunaan dosis ditingkatkan berdasarkan faktor pengali 3,2. Untuk menentukan faktor pengali dapat diketahui dengan rumus sebagai berikut: F= √ F = faktor pengali n = jumlah dosis rentang -1 I= I= = 9,09 ~ 9,1 F = √9,1 = x log 9,1 = x 0,96 = 0,48 ~ 0,5 = anti log 0,5 = 3,16 ~ 3,2 Dosis 1 = 550 mg/kg BB Dosis 2 = 550 x 3,2 = 1760 mg/kg BB, (dosis yang dipakai 1750 mg/kg BB) Dosis 3 = 1750 x 3,2 = 5600 mg/kg BB, (dosis yang dipakai 5000 mg/kg BB)

72

LAMPIRAN E HASIL PERHITUNGAN KADAR ABU SIMPLISIA No

1. 2. 3. I.

W (krus kosong) (gram) 33,4309 33,9405 32,6390

W (bahan) (gram) 2,0077 2,0053 2,0087

Kadar Abu =

(

)

= II.

Kadar Abu =

,

, )

,

,

(

)

=

Rata – Rata =

,

,

,

,

= 4,44%

73

4,44% ± 0,135

× 100%

×100%

× 100% ,

,

Rata – rata ± SD

× 100% = 4,30%

,

Kadar Abu =

% Kadar Abu 4,57 4,30 4,45

× 100% = 4,57%

,

(

= III.

W (krus + abu) (gram) 33,5226 33,0268 32,7283

× 100% = 4,45

LAMPIRAN F HASIL PERHITUNGAN KADAR AIR SIMPLISIA No.

W (cawan W (bahan)W (cawan + % Kadar Rata – rata± SD kosong)

(gram)

simplisia)

(gram)

Air

(gram)

1.

119,0803

2,0119

120,9250

8,31%

2.

85,2373

2,0049

87,0713

8,49%

I.

–

Kadar Air = =

,

,

–

=

,

× 100% × 100% = 8,31%

,

II. Kadar Abu =

Rata – Rata =

8,40%± 0,127

,

,

, ,

= 8,40%

74

× 100% × 100% = 8,49%

LAMPIRAN G HASIL PERHITUNGAN KADAR SARI LARUT AIR SIMPLISIA No.

W (Cawan W (bahan) kosong)

W

% Kadar Sari Rata – rata± SD

(gram)

Larut (cawan +

(gram)

Etanol

filtrat) (gram) 1.

83,5523

5,0037

83,7050

15,26%

2.

79,1089

5,0036

79,2652

15,62%

3.

57,3892

5,0052

57,5441

15,47%

1. Kadar Sari Larut Etanol =

(

= 2.Kadar Sari Larut Etanol =

)– ,

,

(

)–

=

,

,

(

)– ,

=

,

,

, ,

,

= 15,45%

75

× 100% × 100% = 15,26% ×100%

×

,

3.Kadar Sari Larut Etanol =

Rata – Rata =

×

,

15,45% ± 0,18

× 100% = 15,62% × 100%

×

× 100% = 15,47%

LAMPIRAN H HASIL PERHITUNGAN KADAR SARI LARUT ETANOL SIMPLISIA No. W (CawanW (bahan) kosong)

W

% Kadar Sari Rata – rata± SD

(gram) (cawan +

(gram)

Larut Air

filtrat) (gram)

1.

83,5494

5,0028

83,6828

13,33%

2.

83,1287

5,0079

83,2676

13,87%

1.Kadar Sari Larut Air =

(

)–

=

,

×

,

(

2.Kadar Sari Larut Air =

)–

=

Rata – Rata =

,

,

,

,

×

,

,

= 13,60%

76

13,60%± 0,38

× 100% × 100% = 13,33 % ×100% × 100% = 13,87 %

77

LAMPIRAN I SKRINING FITOKIMIA

A.

B.

(D)

C.

(E)

Keterangan : A. Alkaloid dengan pereaksi dragendroff B. Flavonoid dengan penambahan serbuk Mg, 2 ml alkohol khlorhidrik, dan amil alkohol C. Saponin D. Tanin dengan pereaksi FeCL3 dan larutan gelatin E. Terpenoid atau steroid

78

LAMPIRAN J HASIL PERHITUNGAN KADAR ABU EKSTRAK No

1. 2. I.

W (krus kosong) (gram) 31,7720 33,3549

W (bahan) (gram) 2,0009 2,0052

Kadar Abu =

(

)

= II.

W (krus + abu) (gram) 31,8652 33,4415

,

,

(

)

=

Rata – Rata =

,

,

,

= 4,48%

79

4,48% ± 0,226 ×100%

× 100% ,

,

Rata – rata ± SD

× 100% = 4,64%

,

Kadar Abu =

% Kadar Abu 4,64% 4,32%

× 100% = 4,32%

LAMPIRAN K HASIL PERHITUNGAN KADAR ABU TIDAK LARUT AIR No

1. 2. I.

W (krus kosong) (gram)

Bobot abu awal (gram)

33,3549 31,7720

2,0052 2,0009

W (Krus + bahan) (gram) 33,4415 31,8652

Kadar Abu =

(

= II.

Kadar Abu =

,

,

% Kadar Abu Larut Air 3,68% 3,34%

) ,

,

, ,

×100% × 100% = 3,34%

= 3,51%

\

80

3,51% ±0,24 × 100%

) ,

Rata – rata ± SD

× 100% = 3,68%

,

(

=

Rata – Rata =

W (Krus + kertas saring) (gram) 33,3677 31,7983

LAMPIRAN L HASIL PERHITUNGAN KADAR ABU TIDAK LARUT ASAM No

W (krus kosong) (gram)

Bobot abu awal (gram)

1. 2.

32,1937 31,7524

2,0097 2,0087

I.

W (Krus + bahan) (gram) 32,2276 31,7941

Kadar Abu =

32,2013 31,7629

(

)

= II.

W (Krus + kertas saring) (gram)

,

,

(

)

Rata – Rata =

,

,

,

Rata – rata ± SD

1,43% ±0,170 ×100%

× 100% = 1,31%

,

Kadar Abu =

=

% Kadar Abu Larut Air 1,31% 1,55%

, ,

= 1,43%

81

× 100% × 100% = 1,55%

LAMPIRAN M HASIL PERHITUNGAN KADAR AIR EKSTRAK No. W (cawan W (Zat) W (cawan +% Kadar Rata – rata± SD kosong)

(gram)

simplisia)

(gram)

Air

(gram)

1.

83,5648

5,0054

88,1658

8,01%

2.

88,1440

5,0012

92,7140

8,62%

3.

79,0626

5,0051

83,6179

8,98%

I.

× 100%

Kadar Air = ,

= II.

,

× 100% = 8,01%

,

×100%

Kadar Abu = ,

= III.

8,54%± 0,490

,

× 100% = 8,62%

,

× 100%

Kadar Abu ,

=

Rata – Rata =

,

,

, ,

,

= 8,54%

82

× 100% = 8,98%

LAMPIRAN N PERHITUNGAN PENIMBANGAN EKSTRAK Berat Badan Sebelum Perlakuan (gram) Kelompok Kelompok Perlakuan 1Kelompok Perlakuan 1 Kelompok Perlakuan 1 Kontrol (Dosis 550 (Dosis 1750 (Dosis 5000 mg/kgBB) mg/kgBB) mg/kgBB) 26 21 33 28 29 29 28 27 28 26 29 36 17 32 29 33

Berikut adalah perhitungan penimbangan ekstrak untuk mencit jantan galur Swiss : Vp =

( (

) /

)

,

Keterangan : Vp : Faktor Pengenceran D

: Dosis

C

: Konsentrasi, berdasarkan rumus diatas maka dapat dihitung :

Dosis 550 mg/kgBB : Vp(2,5ml)=

× 550 : C = 59,4 mg = 0,0594 gram

0,0594 gram = 0,0297 gram/ml X 5 ml = 0,1485 gram/5ml 2 ml Dosis 1750 mg/kgBB : C=

Vp(2,5ml)=

× 1750 : C = 208, 25mg = 0,20825 gram

0,20825 gram = 0,1041 gram/ml X 5 ml = 0,5206 gram/5ml 2 ml Dosis 5000 mg/kgBB : C=

Vp(2,5ml)= C=

× 5000 : C = 620 mg = 0,62 gram 0,62 gram = 0,31 gram/ml X 5 ml = 1,55 gram/5ml 2 ml 83

LAMPIRAN O PENIMBANGAN ORGAN (gram) Kode

BB awal

BB akhir

Bobot Organ (gram) Jantung

Ginjal

Paru

Testis

Limfa

Hati

Vas. Differens

84

Kontrol 1

16

28

0,16

0,48

0,34

0,28

0,42

2,16

0,22

Kontrol 2

17

31

0,14

0,43

0,23

0,23

0,19

1,48

0,22

Kontrol 3

17

31

0,16

0,61

0,29

0,31

0,26

1,99

0,26

Kontrol 4

15

21

0,14

0,23

0,27

0,28

0,24

1,82

0,26

Dosis 550 mg/kgBB

16

25

0,09

0,34

0,19

0,24

0,25

1,31

0,15

Dosis 550 mg/kgBB

17

31

0,14

0,45

0,25

0,28

0,31

2,07

0,38

Dosis 550 mg/kgBB

16

32

0,16

0,33

0,35

0,34

0,13

1,51

0,27

Dosis 550 mg/kgBB

19

34

0,16

0,48

0,24

0,24

0,15

1,44

0,18

Dosis 1750 mg/kgBB

20

35

0,15

0,48

0,22

0,34

0,24

2,05

0,37

Dosis 1750 mg/kgBB

17

30

0,14

0,50

0,24

0,26

0,24

0,94

0,16

Dosis 1750 mg/kgBB

18

33

0,13

0,51

0,25

0,25

0,48

1,75

0,21

Dosis 1750 mg/kgBB

18

32

0,11

0,38

0,31

0,23

0,13

1,39

0,27

Dosis 5000 mg/kgBB

17

29

0,16

0,49

0,26

0,29

0,22

1,84

0,36

Dosis 5000 mg/kgBB

17

29

0,12

0,44

0,21

0,23

0,22

1,75

0,19

Dosis 5000 mg/kgBB

20

34

0,14

0,46

0,32

0,23

0,26

1,76

0,31

Dosis 5000 mg/kgBB

19

34

0,13

0,48

0,24

0,27

0,16

1,49

0,35

85

LAMPIRAN P PERHITUNGAN INDEKS ORGAN (%) Jantung

Ginjal

0,16 × 100 28 = 0,57

Kontrol 1

Paru

0,48 28 × 100 = 1,71

0,34 × 100 28 = 1,21

Testis

Limpa

0,28 × 100 28 = 1,00

0,42 × 100 28 = 1,50

Hati

Vas Differens

2,16 28 × 100 = 7,71

0,22 × 100 28 = 0,79

0,45

1,39

0,74

0,74

0,61

4,77

0,71

Kontrol 3

0,52

1,97

0,94

1,00

0,84

6,42

0,84

Kontrol 4

0,67

1,95

1,29

1,33

1,14

8,67

1,24

86

Kontrol 2

0,36

1,36

0,76

0,96

1,00

5,24

0,60

Dosis 550 mg/kgBB

0,45

1,45

0,81

0,90

1,00

6,67

1,22

Dosis 550 mg/kgBB

0,50

1,22

1,30

1,26

0,48

5,59

1,00

Dosis 550 mg/kgBB

0,47

1,41

0,71

0,70

0,44

4,24

0,53

0,43

1,37

0,63

0,97

0,69

5,86

1,06

Dosis 1750 mg/kgBB

0,47

1,67

0,80

0,87

0,80

3,13

0,53

Dosis 1750 mg/kgBB

0,39

1,55

0,76

0,76

1,45

5,30

0,64

Dosis 550 mg/kgBB

Dosis 1750 mg/kgBB

0,34

Dosis 1750 mg/kgBB

1,19

0,97

0,72

0,41

4,34

0,84

0,55

1,69

0,90

1,00

0,76

6,34

1,24

Dosis 5000 mg/kgBB

0,41

1,52

0,72

0,79

0,76

6,03

0,66

Dosis 5000 mg/kgBB

0,41

1,35

0,94

0,68

0,76

5,18

0,91

Dosis 5000 mg/kgBB

0,38

1,41

0,71

0,79

0,47

4,38

1,03

Dosis 5000 mg/kgBB

87

LAMPIRAN Q HASIL PENGAMATAN KELOMPOK KONTROL

Keterangan : + : Ada Aktivitas : Tidak Ada Aktivitas Kj : Kuning Jernih P : Padat

88

89

LAMPIRAN R HASIL PENGAMATAN KELOMPOK PERLAKUAN DOSIS 550 mg/kgBB

90

91

LAMPIRAN S HASIL PENGAMATAN KELOMPOK PERLAKUAN DOSIS 1750 mg/kgBB

92

93

LAMPIRAN T HASIL PENGAMATAN KELOMPOK PERLAKUAN DOSIS 5000 mg/kgBB

94

95

LAMPIRAN U ORGAN KELOMPOK KONTROL DAN KELOMPOK PERLAKUAN Kontrol 1

Kontrol 2

96

Kontrol 3

Kontrol 4

97

Dosis 550 mg/kgBB (5)


98



99



100



101



102



103

LAMPIRAN V LAMBUNG KELOMPOK KONTROL DAN KELOMPOK PERLAKUAN Kontrol

Perlakuan Dosis 550 mg/kgBB



104

LAMPIRAN W PERHITUNGAN JUMLAH JENGUKAN DENGAN ONE WAY ANOVA

Test of Homogeneity of Variances Jumlah_Jengukan Levene Statistic

df1

df2

1.556

3

Sig. 12

.251

ANOVA Jumlah_Jengukan Sum of Squares Between Groups Within Groups Total

Df

8.188 50.250 58.438

Mean Square 3 12 15

F

Sig.

2.729 .652 .597 4.188

Test of Homogeneity of Variances Jumlah_jengukan Levene Statistic

df1

7.643

df2

Sig.

3

12

.004

ANOVA Jumlah_jengukan Sum of Squares Between Groups Within Groups Total

10.188 37.750 47.938

Df 3 12 15

Mean Square 3.396 3.146

Test of Homogeneity of Variances 102

F 1.079

Sig. .395

Jumlah_Jengukan Levene Statistic

df1

df2

.770

3

Sig. 12

.533

Mean Square

F


Df

15.500 23.500 39.000

3 12 15

Sig.

5.167 2.638 .097 1.958

Test of Homogeneity of Variances Jumlah_Jengukan Levene Statistic .404

df1

df2 3

Sig. 12

.753

ANOVA Jumlah_Jengukan Sum of Squares

Mean Square

Df

Between Groups

29.500

3

Within Groups Total

11.500 41.000

12 15

103

F

9.833 10.26 1 .958

Sig. .001

Post Hoc Tests Multiple Comparisons Jumlah_Jengukan Tukey HSD

(I) Parameter

(J) Parameter

Kontrol

Dosis 550 mg/kgBB

Mean Difference (I-J)

Dosis 1750 mg/kgBB Dosis 5000 mg/kgBB Dosis 550 mg/kgBB

104

Dosis 1750 mg/kgBB

Dosis 5000 mg/kgBB

Kontrol

95% Confidence Interval Std. Error

Sig.

Lower Bound

Upper Bound

-.750

.692

.706

-2.81

*

.692

.016

-4.56

-.44

1.250

.692

.317

-.81

3.31 2.81

-2.500

1.31

.750

.692

.706

-1.31

Dosis 1750 mg/kgBB

-1.750

.692

.105

-3.81

.31

Dosis 5000 mg/kgBB

2.000

.692

.057

-.06

4.06

2.500*

.692

.016

.44

4.56

Dosis 550 mg/kgBB

1.750

.692

.105

-.31

3.81

Dosis 5000 mg/kgBB

3.750*

.692

.001

1.69

5.81

Kontrol

-1.250

.692

.317

-3.31

.81

Dosis 550 mg/kgBB

-2.000

.692

.057

-4.06

.06

Dosis 1750 mg/kgBB

-3.750*

.692

.001

-5.81

-1.69

Kontrol

*. The mean difference is significant at the 0.05 level.

Homogeneous Subsets Jumlah_Jengukan Tukey HSDa Subset for alpha = 0.05 Parameter

N

1

2

Dosis 5000 mg/kgBB 4 1.50 Kontrol 4 2.75 Dosis 550 mg/kgBB 4 3.50 Dosis 1750 mg/kgBB 4 Sig. .057 Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 4,000.

3.50 5.25 .105


df1

1.493

df2

Sig.

3

12

.266


23.500 36.500 60.000

Df

Mean Square

3 12 15

105

F

7.833 2.575 3.042

Sig. .103


df1

df2

1.338

Sig.

3

12

.308


Mean Square

df

32.188 57.250 89.438

3 12 15

F

10.729 4.771

Sig.

2.249

.135


df1

2.267

df2 3

Sig. 12

.133


13.688 72.750 86.438

Mean Square

Df 3 12 15

106

4.563 6.063

F .753

Sig. .542


df1

1.811

df2 3

Sig. 12

.199


7.188 42.250 49.438

Mean Square

Df 3 12 15

107

2.396 3.521

F .680

Sig. .581

LAMPIRAN X PERHITUNGAN WAKTU MENGGELANTUNG DENGAN ONE WAY ANOVA

Test of Homogeneity of Variances Waktu_Menggelantung Levene Statistic

df1

df2

5.697

Sig.

3

12

.012

ANOVA Waktu_Menggelantung Sum of Squares Between Groups Within Groups Total

Mean Square

df

72.610

3

24.203

258.031 330.640

12 15

21.503

F

Sig.

1.126

.378


df1

34.762

df2

Sig.

3

12

.000


241.470 625.710 867.180

df

Mean Square

3 12 15

108

80.490 52.142

F 1.544

Sig. .254

Test of Homogeneity of Variances Waktu_menggelantung Levene Statistic

df1

df2

.492

Sig.

3

12

.694

ANOVA Waktu_menggelantung Sum of Squares Between Groups Within Groups Total

Mean Square

Df

82.228 820.385 902.613

3 12 15

F

27.409 68.365

Sig.

.401

.755


df1

df2

.617

3

Sig. 12

.617

Mean Square

F


8.383 489.224 497.607

Df

3 2.794 12 40.769 15

109

.069

Sig. .976


df1

6.000

df2 3

Sig. 12

.010


Mean Square

df

F

30.583

3

10.194

183.493 214.076

12 15

15.291

Sig.

.667

.588


df1

3.882

df2 3

Sig. 12

.038


57.756 535.608 593.364

Mean Square

df 3 12 15

F

19.252 44.634

Test of Homogeneity of Variances Waktu_Menggelantung Levene Statistic 3.747

df1

df2 3

Sig. 12

110

.041

.431

Sig. .734

ANOVA Waktu_Menggelantung Sum of Squares Between Groups

Df

Mean Square

60.182

3

20.061

Within Groups

578.158

12

48.180

Total

638.339

15

F

Sig.

.416

.744


df1

.711

df2

Sig.

3

12

.564


Df

Mean Square

208.195

3

69.398

1097.507 1305.701

12 15

91.459

111

F .759

Sig. .538

LAMPIRAN Y PERHITUNGAN INDEKS ORGAN DENGAN ONE WAY ANOVA

Test of Homogeneity of Variances Organ_Ginjal Levene Statistic

df1

.573

df2 3

Sig. 12

.644

ANOVA Organ_Ginjal Sum of Squares Between Groups Within Groups Total

Mean Square

Df

.408 .525 .933

3 12 15

F

.136 .044

3.109

Sig. .067

Test of Homogeneity of Variances Organ_Hati Levene Statistic

df1

.432

df2 3

Sig. 12

.734

ANOVA Organ_Hati Sum of Squares Between Groups Within Groups Total

9.453 17.812 27.265

Mean Square

Df 3 12 15

112

3.151 1.484

F 2.123

Sig. .151

Test of Homogeneity of Variances Organ_Jantung Levene Statistic

df1

df2

.321

3

Sig. 12

.810

ANOVA Organ_Jantung Sum of Squares Between Groups Within Groups Total

df

.046 .062 .108

Mean Square

3 12 15

F

.015 2.990 .005

Sig. .073

Test of Homogeneity of Variances Organ_Limpa Levene Statistic

df1

1.367

df2 3

Sig. 12

.300

ANOVA Organ_Limpa Sum of Squares Between Groups Within Groups Total

Mean Square

df

.281 1.421 1.702

3 12 15

F

.094 .118

.792

Test of Homogeneity of Variances Organ_Paru Levene Statistic 1.711

df1

df2 3

Sig. 12

113

.218

Sig. .521

ANOVA Organ_Paru Sum of Squares Between Groups Within Groups Total

Mean Square

df

.163 .378 .541

3 12 15

F

.054 .032

1.724

Sig. .215

Test of Homogeneity of Variances Organ_Testis Levene Statistic

df1

df2

.330

3

Sig. 12

.804

ANOVA Organ_Testis Sum of Squares Between Groups Within Groups Total

Mean Square

df

.121 .301 .422

3 12 15

F

.040 .025

Test of Homogeneity of Variances Organ_vasDifferen Levene Statistic .185

df1

df2 3

Sig. 12

114

.904

1.611

Sig. .239

ANOVA Organ_vasDifferen Sum of Squares Between Groups Within Groups Total

Mean Square

df

.094 .797 .892

3 12 15

115

.031 .066

F .473

Sig. .707

LAMPIRAN A SURAT DETERMINASI TANAMAN PUTRI MALU

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