94
DAFTAR PUSTAKA Accevedo E, Fereres E. 1993. Resistance to abiotic stress. Di dalam: Hayward MD, Bosemark NO, Romagosa I, editor. Plant Breeding: Principles and Prospect. London: Chapman and Hall. hlm 281-313. Agustina M. 2004. Daya gabung dan pewarisan karakter agronomik padi gogo (Oryza sativa L.) pada tanah podsolik merah kuning [tesis]. Bogor: Program Pascasarjana, Institut Pertanian Bogor. Ambreen A, Chowdhry MA, Khaliq I, Ahmad R. 2002. Determination for some drought related leaf traits in bread wheat. Asian Journal of Plant Sciences 1 (3): 232-234. Andersen SB, Torp AM. 2002. QTL Mapping In Crop Plants. Di dalam: Mohan Jain, Brar DS, Ahloowalia BS, editor. Molecular Techiniques in Crop Improvement. Dordrecht: Kluwer Academic Publisher. hlm 9-54. Anderson JM, Osmond CB. 1987. Sun-shade Responses: Compromises Between Acclimation And Photoinhibition. Di dalam: Kyle DJ, Osmond CB, Arntzen CJ, editor. Topics in Photosynthesis: Photoinhibition. hlm 1-30. Archana MN, Narkhede BD, Ghorade RBG, Golhar SR. 1999. Path coefficient analysis in soybean (Glycine max L. Merril). J Soils and Crop 9: 250-251 Arsyad DM. 2000. Varietas Unggul dan Strategi Pemuliaan Kedelai di Indonesia. Prosiding lokakarya: penelitian dan pengembangan produksi kedelai di Indonesia. Direktorat Teknologi Lingkungan, badan Pengkajian dan Penerapan Teknologi. hlm 39-42. Arus P, Moreno-Gonzales J. 1993. Marker-assisted selection. Di dalam: Hayward MD, Bosemark NO, Romagosa I, [editor]. Plant Breeding: Principles and Prospect. London: Chapman and Hall. hlm 315-331. Asadi B, Arsyad DM, Zahara H, Darmijati. 1997. Pemuliaan kedelai untuk toleran naungan. Buletin Agrobio 1(2):15-20. Austin RB. 1993. Augmentd yield-based selection. Di dalam: Hayward MD, Bosemark NO, Romagosa I, editor. Plant Breeding: Principles and Prospect. London: Chapman and Hall. hlm 391-405. Azrai M, Murdaningsih HK, Neni R, Muljopawiro S, Ruswandi D. 2002. QTL mapping of maize resistance to downy mildew in Bogor. Zuriat 13(2): 113120. [Balitbang Deptan] Badan Penelitian dan Pengembangan Departemen Pertanian. 2005a. Rencana Aksi Pemantapan Ketahanan pangan 2005-2010. Lima Komoditas: Beras, Jagung, kedelai, Gula, dan daging Sapi. Jakarta: Balitbangtan Deptan.
95
[Balitbang Deptan] Badan Penelitian dan Pengembangan Departemen Pertanian. 2005b. Prospek Agribisnis dan Arah Pengembangan Agribisnis Kedelai. Jakarta: Balitbangtan Deptan. Baharsjah JS. 1980. Pengaruh naungan pada berbagai tahap perkembangan dan populasi tanaman terhadap pertumbuhan, hasil dan komponen hasil kedelai (Glycine max (L.) Merr) [disertasi]. Bogor: Program Pasca Sarjana, Institut Pertanian Bogor. Baker RJ. 1993. Breeding methods and selection indices for improved tolerance to biotic and abiotic stresses in cool season food legumes. Euphytica 73 (1-2): 67-72. Bari A. 1998. Pengujian sebaran frekuensi hasil padi dalam pertanaman tumpang sari padi dengan jagung dan ubi kayu. Comm Ag 4(1): 41-45. Bernardo R. 2002. Breeding for Quantitative Minnnesota: Stemma Press.
Traits in Plants. Woodbury,
Bizeti HS, de Carvalho CGP, Souza J, Destro D. 2004. Path analysis under multicollinearity in soybean. Brazilian Archives of Biology and Technology Journal 47(5): 669-676. Brar DS. 2002. Molecular Marker Assisted Breeding. Di dalam: Mohan Jain S, Brar DS, Ahloowalia BS, [editor]. Molocular Techniques in Crop Improvement. Dordrecht: Kluwer Academic Publisher. hlm 55-83. Burnham KD, Dorrance AE, Van Toai TT, St. Martin SK. 2003. Quantitative trait loci for partial resistance to Phytophthora in soybean. Crop Sci 43:1610-1617. Burton JW. 1987. Quantitative Genetics Result Relevant To Soybean Breeding. Di dalam: Wilcox JR, editor. Soybeans: Improvement, Production, and Uses (2nd). Wisconsin. hlm 212-247. Ceccarelli S. 1994. Specific adaptation and breeding for marginal conditions. Euphytica 77: 205-209. Ceccarelli S. 1996. Adaptation to low/high input cultivation. Euphytica 92: 203214. Ceccarelli S, Erskine W, Humblin J, Brando S. 2007. Genotype by environment interaction and international breeding program. http://www.icrisat.com. [15 Januari 2007]. Chahal GS, Gosal SS. 2003. Principles and Procedures of Plant Breeding: Biotechnological and Conventional Approaches. Kolkata: Narosa Publishing House.
96
Changrong Y, Sripichitt P, Hongtrakul V, Juntakool S, Sripichitt A. 2006. A QTL controlling the field resistance of soybean. SABRAO Journal of Breeding and Genetics 38 (2): 93-103. Chaturvedi GS, Ram PC, Singh AK, Ram P, Ingram KT, Singh BB, RK Singh, Singh VP. 1996. Carbohydrat Status Of Rainfed Lowland Rice In Relation To Submergence, Drought, And Shade Tolerance. Di dalam: Lucknow VP, editor. Physiology of Stress Tolerance in Rice. Philippines: India-IRRI. hlm 104 – 122. Cho Y, Scott RA. 2000. Combining ability of seed vigor and seed yield in soybean. Euphytica 112:145-150. Chowdry MA, Ambreen A, Khaliq I. 2002. Genetic of some polygenic traits in Aestivum species. Asian Journal of Plant Sciences 1 (3):235-237. Chozin MA, Sopandie D, Sastrosumarjo S, Suwarno. 1999. Physiology and genetic of upland rice adaptation to shade. Final Report of Graduate Team Reseach Grant, URGE Project. Jakarta: Directorate General of Higher Education, Ministry of Education and Culture. Chozin MA, Sopandie D, Sastrosumarjo S, Suwarno. 1998. Physiology and Genetic of Upland Rice Adaptation to Shade. Final Report of Graduate Team Reseach Grant, URGE Project. Jakarta: Directorate General of Higher Education, Ministry of Education and Culture. Conner AJ, Keizer LCP, Mlynarova L, Stiekema WJ, Nap J-P. 1998. Evaluation of diallel analysis using β-glucuronidase activity from transgenes in Nicotiana tabacum. Euphytica 102:161-168. Daubenmire RF. 1974. Plants and Environments: A Texbook of Plant Autoecology. Ed ke-3. New York: Ed Jhon Wiley. Elfarisna. 2000. Adaptasi kedelai terhadap naungan: studi morfologi dan anatomi. [tesis]. Bogor: Program Pascasarjana, Institut Pertanian Bogor. Falconer DS, Mackay TFC. 1996. Introduction to Quantitative Genetics. Ed ke-4. Essex: Longman. Farooq S, Azam F. 2002. Molecular markers in plant breeding-III: practical application and difficulties encountered. Pakistan Journal of Biological Sciences 5 (10): 1148-1154. Forster BP, Ellis RP, Thomas WTB, Newton AC, Tuberosa R, This D, El-Enein RA, Bahri MH, Ben Salem M, 2000. The development and application of molecular markers for abiotic stress tolerance in barley. Experimental Botany 51 (342):19-27. Fronza V, Velo NA, Camargo LEA. 2004. Genetic analysis of soybean resistance to Fusarium solani f. sp. glycines. Genet Mol Biol (27) 3: 1-14.
97
Funatsuki H, Kawaguchi K, Matsuba S, Sato Y, Ishimoto M. 2005. Mapping of QTL associated with chilling tolerance during reproductive growth in soybean. Theoretical and Applied Genetics 111 (5) : 851-861. Gesteira AS, Schuster I, Jose IC, Piovesan ND, Viana JMS, de Barros EG, Moreira MA. 2003. Biometrical analyses of linolenic acid content of soybean seeds. Genetics and Molecular Biology (26) 1: 65-68. Gomez KA, Gomez AA. 1995. Prosedur Statistik untuk Penelitian Pertanian. Sjamsudin E, Baharsjah JS. Jakarta: Universitas Indonesia. Griffing B. 1956. Concept of general and specific of combining ability in relation to diallel crossing systems. Aust J Sci 9:463-493. Gupta PK, Varshney RK, Prasad M. 2002. Molecular Markers: Principles And Methodology. Di dalam: Mohan Jain S, DS Brar, Ahloowalia BS, editor. Molocular Techniques in Crop Improvement. Dordrecht: Kluwer Academic Publisher. hlm 9-54. Haldane JBS. 1919. The Combination of linkage value and the calculation of distance between the loci of linked factors. J Genet 8:299-309. Hale MG, Orcutt DM. 1987. The Physiology of Plant under stress. New York: Jhon Wiley and Sons. Handayani T. 2003. Pola pewarisan sifat toleransi terhadap intensitas cahaya rendah pada kedelai (Glycine max (L.) Merril) dengan penciri spesifik karakter anatomi, morfologi, dan molekuler [disertasi]. Bogor: Program Pasca Sarjana, Institut Pertanian Bogor. Hayman BI. 1954. The theory and analysis of diallel crosses. Genetics 39: 701809. Hoeck JA, Walter RF, Randy CS, Grace AW, Susan LJ, SR Cianzio. 2003. Molecular marker analysis of seed size in soybean. Crop Sci 43: 68-74. Hussain SS. 2006. Molecular breeding for abiotic stress tolerance: drought perspective. Proc Pakistan Acad Sci 43 (3): 189-210. Iqbal MJ, Meksem K, Njiti VN, My A Kasem. 2001. Microsatelite markers identify three additional quantitative trait loci for resistance to soybean sudden death syndrome (SDS) in Essex. Forest. Theor Appl Genet 102:187192. Iqbal S, Ariq M, Tahira M, Ali M, Anwar M, Sarwar M. 2003. Path coefficient analysis in different genotypes of soybean (Glycine max (L.) Merr). Pakistan Journal of Biological Sciences 6 (12): 1085-1087. Irwansyah E. 2004. Peta pautan genetik marka rapd dan analisis qtl kelapa sawit menggunakan populasi silang balik generasi pertama menuju perbaikan
98
kualitas minyak [disertasi]. Bogor: Program Pasca Sarjana, Institut Pertanian Bogor. Ishitani M, Rao I, Wenzl P, Beebe S, Tohme J. 2004. Integration of genomics approach with traditional breeding toward improving abiotic stress adaptation: drought and aluminium toxicity as case study. Field Crops Research 90: 35-45. Isler N, Caliskan ME. 1998. Correlation and path coefficient analysis for yield and some yield components of soybean (Glycine max L. Merr) grown in South eastern Anatolia. Turkish J Agri Res 116:5-8. Kelker D, Kelker H. 1986. The effect of skewness on selection in a plant breeding program. Euphytica 35 (1): 303-309. Khan SA, Habib I. 2003. Gene action in a five parents diallel cross of spring wheat (Triticum aestivum L.). Pakistan Journal of Biological Sciences 6 (23): 1945-1948. Khanghah, Sohani. 1999. Genetic evaluation of some important agronomic traits related to seed yield by multivariete of soybean analysis methods. Iranian J Agri Sci 30: 807-816. Khumaida N. 2002. Studies on upland rice and soybean to shade strees [disertasi]. Tokyo: Graduate School of Agricultural and Life Science, The University of Tokyo. Khush GS. 2002. Molecular genetics-plant breeders’s perspective. Di dalam: Mohan Jain S, Brar DS, Ahloowalia BS, editor. Molecular Techniques in Crop Improvement. Dordrecht: Kluwer Academic Publisher. hlm 1-8. Kisman, Khumaida N, Trikoesoemaningtyas, Sobir, Sopandie D. 2007. Respon Molekuler Tanaman Kedelai terhadap Cekaman Intensitas Cahaya Rendah: Analisis Ekspresi Gen ATHB dan CAB. Prosiding Seminar Nasional Bioteknologi dan Pemuliaan Tanaman; Dept AGH, Faperta IPB Bogor, 1-2 Agustus 2006. Kisman. 2007. Analisis genetik dan molekuler adaptasi kedelai terhadap intensitas cahaya rendah berdasarkan karakter morfo-fisiologi daun [disertasi]. Bogor: Program Pasca Sarjana, Institut Pertanian Bogor. Korzun V. 2004. Molecular marker and their application in cereal breeding. Proceeding of the seminar Marker Assisted Selection: A Fast Track to Increase Genetic Gain In Plant and Animal Breeding. Einbeck, Germany. La Muhuria. 2007. Mekanisme fisiologi dan pewarisan sifat toleransi kedelai (glycine max l. (merril) terhadap intensitas cahaya rendah [disertasi]. Bogor: Program Pasca Sarjana, Institut Pertanian Bogor.
99
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L. 1987. Mapmaker: an interactive package for constructing primary linkage map of experimental and natural populations. Genomics 1:174-181. Levitt J. 1980. Response of Plant to Environmental Stress. New York: Academic Press. Lincoln S, Daly MJ, Lander ES. 1992. Constructing linkage map with MAPMAKER/EXP 3.0. Whitehead Institute. Research Technical Report. Liu BH. 1998. Statistical Genomics: Linkage, Mapping, and QTL Analysis. Boca: CRC Press. Makmur A. 1992. Pengantar Pemuliaan Tanaman. Jakarta: Riweka Cipta. Mather SK, Jinks JL. 1982. Biometrical Genetics: study of continuous variation. Ed ke-3 . London: Chapman and Hall. Maughan PJ, Saghai-Maroof MA, Buss GR. 2003. Identification of quantitative trait loci controlling sucrose content in soybean (Glycine max). Molecular Breeding 6:105-111. Mohan, M., S. Nair, A. Bhagwat, T. G. Krishna, Masahiro Y. 1997. Genom mapping, molecular markers and markers assisted selection in crop plants. Molecular Breeding. 3: 87-103. Moreno-Gonzales J, Cubero JI. 1993. Selection strategies and choice of breeding methods. Di dalam: Hayward MD, Bosemark NO, Romagosa I, editor. Plant Breeding: Principles and Prospect. London: Chapman and Hall. hlm 281-313. Nindra M. 2005. Evaluasi keragaman dan pendugaaan keragaman genetik populasi kedelai (Glycine max (L.) Merr) generasi F5 [skripsi]. Bogor: Fakultas Pertanian, Institut pertanian Bogor. Nugraha US, Damardjati DS, Widowati S. 2002. Pengembangan mutu kedelai untuk agroindustri. Prosiding lokakarya: penelitian dan pengembangan produksi kedelai di Indonesia. Direktorat Teknologi Lingkungan, badan Pengkajian dan Penerapan teknologi. hlm 24-38. Pantalone VR, Burton JW, Carter TE. 1996. Soybean fibrous root heritability and genotipic correlations with agronomic and seed quality traits. Crops Science. 36: 1120-1125 Pinem R. 2000. Strategi pengembangan produksi kedelai. Prosiding Lokakarya Penelitian dan Pengembangan Kedelai di Indonesia. BPP Teknologi, Jakarta; 6-7 Agustus 1996. hlm 11-16
100
Poehlman JM, Sleper DA. 1983. Breeding Field crops. 2nd edition. Publishing Company.
Avi
Poespodarsono S. 1988. Dasar-dasar Ilmu Pemuliaan Tanaman. Pusat Antar Universitas Institut Pertanian Bogor bekerja sama dengan Lembaga Sumberdaya Informasi-IPB. Reyna N, Sneller CH. 2001. Evaluation of marker – assisted introgression of yield QTL alleles into adapted soybean. Crop SC 41: 1317-1321. Romagosa I, Fox PN. 1993. Genotipe x environment interaction and adaptation. Di dalam: Hayward MD, Bosemark NO, Romagosa I, editor. Plant Breeding: Principles and Prospect. London: Chapman and Hall. hlm 373390. Rostini N, Baihaki A, Setiamihardja R, Suryatma G. 2002. Pewarisan karakter kandungan klorofil pada kedelai. Zuriat 2 (2): 25-28. Roy D. 2000. Plant Breeding: Analysis and Exploitation of Variation. Calcutta: Narosa Publishing House. Ruswandi D, Hautea DM, Carpena AL, Lantican RM, Salazar AM, Raymundo AD. 2002. Quantitative trait loci maping of Philippine downy mildew resistance gene in mayze (Zea mays L.). Zuriat 13(1): 27-34. Saleem M, Chowdory MA, Khasif M, Khaliq M. 2005. Inheritance pattern of plant height, grain yield and some leaf characteristic of spring wheat. International Journal of Agriculture and Biology (7) 6: 1015-1018. Salisbury FB, Ross CW. 1992. Plant Physiology. Ed ke-4. Wadsworth Pub. Co. Sambrook J, Fritsch EF, Manuatis T. 1989. Molecular Cloning: a laboratory manual (3). New York: Cold Spring Harbour Lab. Press. Shoemaker RC, Polzin KM, Lorenzen LL, Specht JE. 1996. Molecular Mapping of Soybean. Di dalam: Verma DPS, Shoemaker RC, editor. Soybean: Genetics, Molecular Biology, and Biotechnology . hlm 37-53. Shrivasatava MK, Sukla RS, Jain PK. 2001. Path coefficient analysis in diverse genotype of soybean (Glycine max L). Plant Science 4: 47-51. Silva MP da, Junior AT do A, Rodrigues R, Pereira MG, Viana AP. 2004. Genetic control on morphogenetic traits in snap bean. Journal of Brazilian Archives of Biology and Technology (47) 6: 855-862. Singh RK, Chaudary BD. 1976. Biometrical Methods in Quantitative Genetics Analysis. New Delhi: Kalyani Publisher. Sopandie D, Trikoesoemaningtyas, Sulistyono E, Heryani N. 2002. Pengembangan kedelai sebagai tanaman sela: Fisiologi dan pemuliaan untuk
101
toleransi terhadap naungan. Laporan Penelitian Hibah Bersaing. Dirjen Dikti. Sopandie D, Chozin MA, Sastrosumarjo S, Juaheti T, Sahardi. 2003a. Toleransi terhadap naungan pada padi gogo. Hayati 10:71-75. Sopandie D, Chozin MA, Tjitrosemito S, Sahardi. 2003b. Keefektifan uji cepat ruang gelap untuk seleksi ketenggangan pada padi gogo. Hayati 10:91-95. Sopandie D, Trikoesoemaningtyas, Sulistyono E, Heryani N. 2003c. Pengembangan kedelai sebagai tanaman sela : Fisiologi dan pemuliaan untuk toleransi terhadap naungan. Laporan Penelitian Hibah Bersaing. Dirjen Dikti. Sopandie D, Trikoesoemaningtyas, Khumaida N. 2004. Pembentukan varietas unggul kedelai toleran naungan: fisiologi, genetik, dan molekuler adaptasi terhadap intensitas cahaya rendah. Usulan Penelitian Hibah Penelitian Tim Pascasarjana. Dirjen Pendidikan Tinggi. Mendiknas Sopandie D. 2006. Perspektif fisiologi dalam pengembangan tanaman pangan di lahan marjinal. Orasi Ilimiah Guru Besar Tetap Fisiologi Tanaman. Bogor: Institut Pertanian Bogor. 16 September 2006 Sopandie D, Trikoesoemaningtyas, Khumaida N. 2006. Pembentukan varietas unggul kedelai toleran naungan: Fisiologi, genetik, dan molekuler. Adaptasi terhadap intensitas cahaya rendah. Laporan Penelitian Hibah Penelitian Tim Pascasarjana. Dirjen Pendidikan Tinggi. Mendiknas Specht JE, Graef GL. 1996. Limitations and potentials of genetic manipulation of soybean. Di dalam: Verma DPS, Shoemaker RC, editor. Soybean: Genetics, Molecular Biology and Biotechnology. Wallingfor, UK: CAB International. Specht JE, Chase K, Macrander M, Graef GL, Chung J, Markwell JP, Germann M, Orf JH, Lark KG. 2001. Soybean response to water: a QTL analysis of drought tolerance. Crop Science 41: 493-509. Stanfield WD. 1983. Theory and Problems of Genetics. Ed ke-2. New York: McGraw-Hill. Steel RGD, Torrie HJ. 1993. Principles and Procedurs of Statistics. A Biometrical Approach. New York: McGraw-Hill Book. Co. Stoskopf NC, Tomes DT, Christie BR. 1993. Plant Breeding: Theory and Practice. San Fransisco: Westview Press. Sumarno T, Sarwanto. 2000. Perkembangan Penelitian Budidaya Kedelai di Indonesia. Prosiding Lokakarya: Penelitian dan Pengembangan Produksi Kedelai di Indonesia. Direktorat Teknologi Lingkungan, Badan Pengkajian dan Penerapan Teknologi. Hal 17-25.
102
Surahman M. 2002. Peta genetik tanaman, prinsip dan aplikasinya. Bul Agron 30 (1): 27-30. Taiz L, Zeiger E. 1991. Plant Physiology. California: The Benjamin/Cummings Pub. Co. Inc. Tasma IM, Shoemaker RC. 2003. Mapping flowering time gene in soyben and their association with maturity (E) loci. Crop Sci 43: 319-328. Teng S, Qian Qian, Dali Z, Yasufumi K, Kan F, Danian H., Lihuang Z. 2004. QTL analysis of leaf photosynthetic and related physiological traits in rice (Oryza sativa L.). Euphytica 135:1-7. Terry LI, Chase K, Jarvic T, Orf J, Mansur L, KG Lark. 2000. Soybean quantitative trait loci for resistance to insect. Crop Science 40: 375-381. Tester M, Bacic A. 2005. Abiotic stress tolerance in grasses. From model plants to crops plants. Plant Physiology 137:791-793. Toruan-Mathius N, Hutabarat T. 1997. Analysis of genetic integrity of banana planlets from in vitro culture by random amplified polymorphic DNA (RAPD). Menara Perkebunan 65 (1):17-25. Trikoesoemaningtyas. 2002. Fisiologi dan pewarisan sifat efisiensi kalium dalam keadaan aluminium pada padi gogo (oryza sativa l.) [tesis]. Bogor: Sekolah Pasca Sarjana, Institut Pertanian Bogor. Trikoesoemaningtyas, Sopandie D, Takano T. 2003. Genetic and Breding of Soybean for adaptation to shade stress. Proceeding of the 3nd Seminar Toward Harmonization between Development and Environmental Conservation in Biological Production. Tokyo: Tokyo University, 15-16 Pebruari 2003. Trikoesoemaningtyas, Wirnas D, Sopandie D, Takano T. 2004. Development of selection criteria for the selection of shade-tolerant soybean lines. Proceeding of the 4nd Seminar Toward Harmonization between Development and Environmental Conservation in Biological Production. Banten, 2-4 Desember 2004. Tukamuhabwa P, Rubaihayo P, Dashiel KE. 2002. Genetic component of pod shattering in soybean. Euphytica 125: 29-34. Utz HF, Melchinger AE. 2003. PLABQTL: A Computer Program to Map QTL. Institute of Plant Breeding, Seed Science and Population Genetics. Stuttgart: University of Hohenheim. Vello NA, Luis EAC. 2004. Genetic analysis of soybean resistance to Fusarium solani f. sp.glycines. Genet Mol Biol 27(3):1-14.
103
Vieira AJD, de Olivera DA, Soares TCB, Schuster I, Piovesan ND, Martinez CA, de Barros EG, Moreira MA. 2006. Use of the QTL to the study of soybean trait relationship in two population of recombinant inbred lines at the F7 and F8 generations. Braz J Plant Physiol 18 (2): 281-290. Wang D, Graef GL , Procopiuk AM , Diers BW. 2004. Identification of putative QTL that underlie yield in interspecific soybean backcross populations. Theoretical and Applied Genetics. 108 (3): 458-467 Wibowo CS. 2002. Pendugaan Parameter Genetik Karakter Toleran Naungan pada Generasi F2 Hasil Persilangan Kedelai (Glycine max (L.) Merr.). [skripsi]. Bogor : Fakultas Pertanian, Institut Pertanian Bogor. Wijayanti L. 2002. Evaluasi karakter morfologi dan agronomi 12 genotipe f1 kedelai (glycine max (l.) Merr.) pada keadaan ternaungi [skripsi]. Bogor: Fakultas Pertanian, Institut Pertanian Bogor. Winicov I. 2002. Molecular Markers and Abiotic Stresses. Di dalam S Mohan Jain, Brar DS, Ahloowalia BS, editor. Molecular Techniques in Crop Improvement. Dordrecht: Kluwer Academic Publisher. Wirnas D, Trikoesoemaningtyas, Sopandie D. 2005. Seleksi secara simultan terhadap hasil dalam rangka mengembangkan varietas kedelai toleran naungan. Seminar Hasil-Hasil Penelitian Fakultas Pertanian, IPB. Kampus IPB Darmaga, 21-22 September 2005 Wirnas D, Widodo I, Trikoesoemaningtyas, Sobir, Sopandie D. 2006. Pemilihan karakter agronomi untuk menyusun indeks seleksi pada 11 populasi kedelai generasi F6. Bull Agronomi XXXIV (1):19-24. Wrickle G, Weber WE. 1986. Quantitative Genetics and Selection in Plant Breeding. Berlin: Welter de Gruyter. Yuan J, Njiti VN, Meksem K, Iqbal MJ, Triwitayakom K, Kassem MA, Davis GT, Schmidt ME, Lightfoot DA. 2003. Quantitative trait loci in two soybean recombinant inbred line populations segregating for yield and disease resistance. Crop Sci 43:1610-1617.
104
LAMPIRAN
105
Tabel Lampiran 1. Daftar primer yang diseleksi untuk mendapatkan primer yang polimorfik dan terpaut dengan tetua toleran No. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30
Primer OPE01 OPE02 OPE03 OPE04 OPE05 OPE06 OPE07 OPE08 OPE09 OPE10 OPE11 OPE12 OPE13 OPE14 OPE15 OPE16 OPE17 OPE18 OPE19 OPE20 OPH01 OPH02 OPH03 OPH04 OPH05 OPH06 OPH07 OPH08 OPH09 OPH10
Sekuens 5’CCCAAGGTCC’3 5’GGTGCGGGAA’3 5’CCAGATGCAC’3 5’GTGACATGCC’3 5’TCAGGGAGGT’3 5’AAGACCCCTC’3 5’AGATGCAGCC’3 5’TCACCACGGT’3 5’CTTCACCCGA’3 5’CACCAGGTGA’3 5’GAGTCTCAGG’3 5’TTATCGCCCC’3 5’CCCGATTCGG’3 5’TGCGGCTGAG’3 5’ACGCACAACC’3 5’GGTGACTGTG’3 5’CTACTGCCGT’3 5’GGACTGCAGA’3 5’ACGGCGTATG’3 5’AACGGTGACC’3 5’GGTCGGAGAA’3 5’TCGGACGTGA’3 5’AGACGTCCAC’3 5’GGAAGTCGCC’3 5’AGTCGTCCCC’3 5’ACGCATCGCA’3 5’CTGCATCGTG’3 5’GAAACACCCC’3 5’TGTAGCTGGG’3 5’CCTACGTCAC’3
No. 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60
Primer OPH11 OPH12 OPH13 OPH14 OPH15 OPH16 OPH17 OPH18 OPH19 OPH20 OPM01 OPM02 OPM03 OPM04 OPM05 OPM06 OPM07 OPM08 OPM09 OPM10 OPM11 OPM12 OPM13 OPM14 OPM15 OPM16 OPM17 OPM18 OPM19 OPM20
Sekuens 5’CTTCCGCATG’3 5’ACGCGCATGT’3 5’GACGCCACAC’3 5’ACCAGGTTGG’3 5’AATGGCGCAG’3 5’TCTCAGCTGG’3 5’CACTCTCCTC’3 5’GAATCGGCCA’3 5’CTGACCAGCC’3 5’GGGAGACATC’3 5’GTTGGTGGCT’3 5’ACAACGCCTC’3 5’GGCGGATGAG’3 5’GGCGGTTGTC’3 5’GGGAACGTGT’3 5’CTGGGCAACT’3 5’CCGTGACTAC’3 5’TCTGTTCCCC’3 5’GTCTTGCGGA’3 5’TCTGGCGCAC’3 5’GTCCACTGTG’3 5’GGGACGTTGG’3 5’GGTGGTCAAG’3 5’AGGGTACCAC’3 5’GACCTACCAC’3 5’GTAACCAGCC’3 5’TCAGTCCGGG’3 5’CACCATCCGT’3 5’CCTCCAGGCA’3 5’AGGTCTTGGG’3
106
Tabel Lampiran 2. Hasil pengukuran dengan spektrofotometer DNA RILs F6 kedelai hasil persilangan Ceneng dan Godek No. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41
genotipe
λ1
λ2
CG-30-10 CG-28-3 CG-2-2 CG-26-4 CG-56-3 CG-31-7 CG-69-8 CG-69-3 CG-2-1 CG-43-6 CG-60-1 CG-57-8 CG-13-6 CG-31-4 CG-63-1 CG-45-2 CG-5-8 CG-43-10 CG-60-4 CG-9-2 CG-22-6 CG-49-6 CG-18-5 CG-32-4 CG-30-4 CG-27-8 CG-74-10 CG-9-5 CG- 46-2 CG- 22-4 CG-23-10 CG-14-8 CG-17-7 CG-57-4 CG-38-2 CG-3-7 CG-66-8 CG-44-9 CG-17-6 CG-6-2 CG-78-5
0.080 0.190 0.053 0.115 0.047 0.100 0.144 0.088 0.121 0.193 0.141 0.055 0.065 0.122 0.096 0.106 0.186 0.224 0.114 0.106 0.181 0.066 0.211 0.208 0.247 0.196 0.063 0.101 0.246 0.064 0.075 0.163 0.147 0.093 0.085 0.222 0.084 0.231 0.112 0.255 0.068
0.044 0.100 0.029 0.056 0.026 0.053 0.072 0.047 0.064 0.102 0.070 0.030 0.036 0.062 0.053 0.053 0.091 0.124 0.055 0.052 0.095 0.036 0.106 0.108 0.129 0.096 0.034 0.050 0.128 0.036 0.036 0.078 0.075 0.049 0.045 0.114 0.038 0.116 0.053 0.135 0.037
λ1/λ2 1.82 1.90 1.83 2.05 1.81 1.89 2.00 1.87 1.89 1.89 2.01 1.83 1.81 1.97 1.81 2.00 2.04 1.81 2.07 2.04 1.91 1.83 1.99 1.93 1.91 2.04 1.85 2.02 1.92 1.78 2.08 2.09 1.96 1.90 1.89 1.95 2.21 1.99 2.11 1.89 1.84
Konsentrasi DNA (ng/µL) 600.0 1425.0 397.5 862.5 352.5 750.0 1080.0 660.0 907.5 1447.5 1057.5 412.5 487.5 915.0 720.0 795.0 1395.0 1680.0 855.0 795.0 1357.5 495.0 1582.5 1560.0 1852.5 1470.0 472.5 757.5 1845.0 480.0 562.5 1222.5 1102.5 697.5 637.5 1665.0 630.0 1732.5 840.0 1912.5 510.0
V1 (µL) 4.2 1.8 6.3 2.9 7.1 3.3 2.3 3.8 2.8 1.7 2.4 6.1 5.1 2.7 3.5 3.1 1.8 1.5 2.9 3.1 1.8 5.1 1.6 1.6 1.3 1.7 5.3 3.3 1.4 5.2 4.4 2.0 2.3 3.6 3.9 1.5 4.0 1.4 3.0 1.3 4.9
V2 (µL) 95.8 98.2 93.7 97.1 92.9 96.7 97.7 96.2 97.2 98.3 97.6 93.9 94.9 97.3 96.5 96.9 98.2 98.5 97.1 96.9 98.2 94.9 98.4 98.4 98.7 98.3 94.7 96.7 98.6 94.8 95.6 98.0 97.7 96.4 96.1 98.5 96.0 98.6 97.0 98.7 95.1
107
Tabel Lampiran 2. Hasil pengukuran dengan spektrofotometer DNA RILs F6 kedelai hasil persilangan Ceneng dan Godek (lanjutan) No.
genotipe
λ1
λ2
λ1/λ2
Konsentrasi DNA (ng/µL) 1027.5 1080.0 915.0 412.5 922.5 712.5 817.5 1222.5
V1 (µL) 2.4 2.3 2.7 6.1 2.7 3.5 3.1 2.0
V2 (µL) 97.6 97.7 97.3 93.9 97.3 96.5 96.9 98.0
42 CG-80-5 0.137 0.068 2.01 43 CG-29-2 0.144 0.073 1.97 44 CG-23-7 0.122 0.060 2.03 45 CG-53-9 0.055 0.028 1.96 46 CG-21-1 0.123 0.061 2.02 47 CG-29-4 0.095 0.045 2.11 48 CG-76-7 0.109 0.060 1.82 49 CG-76-10 0.163 0.084 1.94 Keterangan : V1= volume DNA yang diperlukan untuk menyiapkan larutan DNA dengan konsentrasi 25 ng/µL V2= volume H2O yang diperlukan untuk memyiapkan 100 µL larutan DNA dengan konsentrasi 25 ng/µL
