DAFTAR PUSTAKA Ackman RG. 1982. Fatty Acid Composition of Fish Oils. Di dalam: Barlow SM and Stasty ME, Editor. Nutritional Evaluation of Long Chain Fatty Acids in Fish Oil. London: Academic Pr. hlm. 25-88. Alexander DW, Mc Guire SO, Cassity NA, Fritsche KL. 1995. Fish oil lower rat plasma and hepatic, but not immune cell α-tocopherol concentration. J Nutr 125:2640-2649. Anggorodi. 1985. Kemajuan Mutakhir dalam Ilmu Makanan Unggas. Jakarta: UI Pr. Bandie MJ. 1982. Status perikanan lemuru di Jawa Timur. Di dalam: Prosiding Seminar Perikanan Lemuru. Banyuwangi, 18 – 21 Januari 1982. Jakarta: Pusat Penelitian dan Pengembangan Perikanan. Badan Penelitian dan Pengembangan Pertanian, Departemen Pertanian. hlm. 37-45. Baumann H, Gauldie J. 1994. The acute phase response. Immunol Today 15: 74-80. Beaur J. 1993. The potential for dietary polyunsaturated fatty acid in domestic animals. Aus Vet J 71:342-345. Bellanti AJ. 1993. Imunologi III. Yogyakarta: UGM Pr. [BNF] British Nutrition Foundation’s. 1994. Unsaturated Fatty Acid, Nutritional and Physiological Significance. London : The Report of The British Nutrition Foundation’s, Task Force. Billiar TR et al. 1988. Fatty acid intake and Kufper cell function: fish oil alters eicosanoid and monokine production to endotoxin stimulation. Surgery 104: 343-349. Boudreau MD, Chanmugan PS, Hart SB, Lee SH, Hwang DH. 1991. Lack of dose response by dietary n-3 fatty acids at a constant ratio of n-3 to n-6 fatty acids in suppressing eicosanoid biosynthesis from arachidonic acid. Am J Clin Nutr 54:111-117. Bremner I , Beattle JH. 1990. Metallothionein and the trace minerals. Am Rev Nutr 10:63-83. Broughton KS, Whelan J, Hardardottir I, Kinsella JE. 1991. Effect of increasing the dietary (n-3) to (n-6) polyunsaturated fatty acid ration on murine liver and peritoneal cell fatty acids and eicosanoid formation. J Nutr 121:155-164. Cahyanto MN, Santoso U, Zuprizal, Irianto HE, Sastrodihardjo S. 1997. Ekstraksi Minyak Mengandung Asam Lemak Omega-3 dari Limbah Industri Minyak Ikan Lemuru dan Penggunaannya dalam Peningkatan Kandungan Asam Lemak Omega-3. [laporan penelitian]. Yogyakarta: Kerjasama Lembaga Penelitian UGM dengan Badan Penelitian dan Pengembangan Pertanian.
84
Chanmugan P et al. 1992. Incorporation of different types of n-3 fatty acids into tissue lipids of poultry. Poult Sci 71:516-521. Decker JM. 2000. Science Inc.
Introduction to Immunology.
Massachusetts; Blackwell
Denbow DM. 2000. Gastrointestinal Anatomy and Physiology. Di dalam: Whittow GC, editor. Sturkie’s Avian Physiology. San Diego: Academy Pr. Dewi EN. 1996. Isolasi Asam Lemak Omega-3 dari Minyak Hasil Limbah Penepungan dan Pengalengan Ikan Lemuru (Sardinella Longiceps) [skripsi]. Bogor: Fakultas Teknologi Pertanian, Institut Pertanian Bogor. Dinarello CA. 1988. Biology of interleukin 1. FASEB J 2:108-115. Dwiponggo A. 1982. Beberapa aspek biologi ikan lemuru, Sardinella longiceps. spp. Di dalam: Prosiding Seminar Perikanan Lemuru. Banyuwangi, 18 – 21 Januari 1982. Jakarta: Pusat Penelitian dan Pengembangan Perikanan. Badan Penelitian dan Pengembangan Pertanian, Departemen Pertanian. hlm. 205-216. Endres S et al. 1989. The effect of dietary supplementation with n-3 polyunsaturated fatty acids on synthesis of interleukin-1 and tumor necrosis factor by mononuclear cells. N Engl J Med 320:265-271. Erf GF, Bottje WG, Bersi TK, Headrick MD, Fritts CA. 1998. Effect of dietary vitamin E on the immune system in broilers: altered proportions of CD4 T cells in the thymus and spleen. Poult Sci 77:529-537. Friedman A, Sklan D. 1995. Effect of dietary fatty acids on antibody production and fatty acid composition of lymphoid organs in broiler chicks. Poult Sci 74: 1463-1469. Friedman A, Sklan D. 1997. Effect of dietary fatty acids on humoral immune response of turkeys. Br Poult Sci 38:342-348. Fritsche KL, Cassity NA, Huang SC. 1991a. Effect of dietary fat source on antibody production and lymphocyte proliferation in chickens. Poult Sci 70: 611-617. Fritsche KL, Cassity NA, Huang SC. 1991b. Effect of dietary fats on the fatty acid compositions of serum and immune tissues in chickens. Poult Sci 70: 1213-1222. Fritsche KL, Cassity NA. 1992. Dietary (n-3) fatty acids reduce antibody dependent cell cytotoxicity and alter eicosanoid release by chicken immune cells. Poult Sci 71:1646-1657. Fritsche KL, Cassity NA, Huang SC. 1992. Dietary (n-3) fatty acid and vitamin E interactions in rats: effects on vitamin E status, immune cell prostaglandin E production and primary antibody response. J Nutr 122:1009-1018.
85
Fritsche KL, Johnston PV. 1990. Effect of dietary omega-3 fatty acids on cellmediated cytotoxic activity in BALB/c mice. Nutr Res 10:577-588. German JB, Lokesh B, Kinsella JE. 1987. Modulation of zymosan stimulated leukotriene release by dietary unsaturated fatty acids. Prostaglandins Leukotrienes Med 30:69-76. Glick B, Olah J. 1993. Bursal secretory dendritic-like cell: a microenvironment issue. Poult Sci 72:1262-1266. Grenfell S, Smither N, Withan S, Sham A. 1991. Analysis of mutation in the putative nuclear localization sequenzes of interleukin-1 B. Biochem J 280: 111-116. Guyton AC. 1983. Fisiologi Kedokteran. Dharma A dan Lukito P, Penerjemah; Jakarta: Penerbit Buku Kedokteran. Terjemahan dari : Medical Fisiology. Hardoko. 1998. Pengaruh Jenis Minyak yang Dikonsumsi terhadap Komposisi Asam Lemak Otak dan Kemampuan Belajar Tikus Percobaan. [disertasi]. Bogor: Program Pascasarjana, Institut Pertanian Bogor. Hamid H, Campbell RS, Parede L. 1991. Studies of pathology of velogenic newcastle disease virus infection in non immune and immune birds. Avian Pathol 20:561-575 Hasmawati, 2005. Studi Histopatologi Organ Paru-paru, Hati, Limpa, dan Ginjal Ayam yang Diinfeksi Eimeria tennela setelah Pemberian Infusa Meniran (Phylanthus niruri L) melalui Air Minum [skripsi]. Bogor : Fakultas Kedokteran Hewan, Institut Pertanian Bogor. Hinds A, Sanders TAB. 1993. The effect of increasing levels of dietary fish oil rich in eicosapentaenoic and docosahexaenoic acids on lymphocyte phospholipid fat acid composition and cell-mediated immunity in the mouse. Br J Nutr 69:423-429. Huang CC, Misfeldt ML, Fritshe KL. 1992. Dietary fat influences la antigen expression and immune cell populations in the murine peritoneum and spleen. J Nutr 122:1219-1231. Husband AJ. 1995. The immune system and intergrated homeostasis. Immunol and Cell Biol 73:377-382. Hwang DH, Beaudreau M, Chanmugan P. 1988. Dietary linolenic acid and longer-chain n-3 fatty acid: comparasion of effect on arachidonic acid metabolism in rats. J Nutr 118:427-437. Kinsella JE, Lokesh B, Broughton S, Whelan J. 1990. Dietary polyunsaturated fatty acids and eicosanoids: Potential effects on the modulation of inflammatory and immune cells. An overview Nutrition 6:24-40. Kizaki H, Suzuki K, Tadakuma T, Ishimura Y, 1990. Adenosin recept-mediated accumulation of cyclic AMP-induced T-lymphocyte death through internucloeosomal DNA cleavage. The J Biol and Biochem 9:5280-5284.
86
Klasing KC. 1984. Effect of inflammatory agents and interleukin-1 on iron and zinc metabolism. Am J Phisiol 247:R901-R904. Klasing KC. 1994. Avian leukocytic cytokines. Poult Sci 73:1035-1043. Klasing KC, Korver DR. 1997. Leukocyte cytokines regulate growth rate and composition following activation of the immune system. J Animal Sci 75 Supl 2: 58 –67. Knudsen PJ, Dinarello CA, Strom TB. 1986. Prostaglandins posttranscrptionally inhibit monocyte expression of interleukin-1 activity by increasing intracellular cyclic adenosine monophosphate. J Immunol 137:3189-3194. Korver DR, Klasing KC. 1997. Dietary fish oil alters specific and inflammatory immune response in chicks. J Nutr 127:2039-2046. Kreutler PA. 1980. Nutrition in Prespective. USA: Prentice-hall, Inc Englewood, Lands WEM. 1986. Fish and Human Health. Chicago: Departement of Biological Chemistry, University of Illionis , Academic Pr, Inc. Leece AA, Allman MA. 1996. The relationships between dietary α-linolenic : linoleic acid and rat platelet eicosapentaenoic and arachidonic acids. British J of Nutr 76:447-452. Lefkowith JB. 1990. Essential fatty acid deficiency: probing the role of arachidonate in biology, Di dalam: Samuelsson B, Dahlen SE, Fritsche SE, Hedqvist P, editor. Advances in Prostaglandin Thromboxane and Leukotriene research. New York : Raven Pr. hlm. 224-231. Lehninger A. 1991. Dasar-Dasar Biokimia. Thenawidjaja M, Penerjemah; Jakarta: Penerbit Erlangga. Terjemahan dari : Principles of Biochemistry. Lowenthal JW, Conaick TE, McWateres PG, York JJ. 1994. Development of T cells immune responsivenes in chicken. Immunol And Cell Biol 72:12941300. Marsh JA. 1993. The humoral activity of the avian thymic microenveroment. Poult Sci 72:1294-1296. Mason JR, Clark L. 2000. The Chemical Senses in Birds. Di dalam: Whittow GC, editor. Sturkie”s Avian Physiology. San Diego: Academic Pr. Meydani M et al. 1991. Effect of longterm fish oil suplementation on vitamin E status and lipid peroxidation in women. J Nutr 121:484-491. Meydani SN et al. 1993. Immunologic effect of national cholesterol education panel step-2 diets with and without fish derived n-3 fatty acid enrichment. J Clin Invest 92:105-113. Meydani SN. 1995. Vitamin E enhancement T cell-mediated function in healthy elderly: mechanisms of action. Nutr Rev Supl 53:52-58.
87
[MFI] Mikrobiologi Farmasi Indonesia. 2007. Sistem Kekebalan Tubuh. http://mikrobia.wordpress.com/2007/03/08/sistem-kekebalan-tubuh068114009068114048068114055/. Navarra P et al. 1992. Interleukin-1β and interleukin-6 specifically increase the release of prostaglandin E2 from rat hypothalamic explants in vitro. Nueroendocrinol 56:61-68. [NRC] National Research Council. 1987. Predicting Feed Intake of FoodProducing Animals. Washington: National Academy Pr. [NRC] National Research Council. 1994. Nutrient Requirement of Poultry. Washington: National Academy Pr. Parakkasi A. 1988. Ilmu Nutrisi dan Makanan Ternak. Volume ke-IB. Jakarta: UI Pr. Pendino KJ, Chepenik KP, Schmidt RR. 1992. Defferential eicosanoid synthesis by murine fetal thymic non-lymphoid cells. Immunol And Cell Biol 70:237-252. Piliang WG. 2002. Nutrisi Vitamin. Volume ke-1. Bogor: Pusat Antar Universitas Ilmu Hayati, Institut Pertanian Bogor. Piliang WG, Djojosoebagio Al Haj S. 2006. Fisiologi Nutrisi. Volume ke-1. Bogor: Pusat Antar Universitas Ilmu Hayati, Institut Pertanian Bogor. Prescott SM. 1984. The effect of eicosapentaenoic acid on leukotriene B production by human neutrophils. J Biol Chem 259:7615-7621. Rodenamm HP, Goldberg. 1982. Arachidonic acid, prostaglandin E2 and F2ά, influence rate of protein turnover in skeletal and cardiac muscle. The J Biol and Biochem 25:1632-1638. Rompannen, T. 1982. Post embryonic development of chicken bursa Fabricius: a light microcopic histoquantitative study. Poult Sci 60:2261-2270. Rusmana D. 2000. Pengaruh Suplementasi Minyak Ikan, Minyak Jagung dan ZnCO3 dalam Ransum terhadap Kandungan “ω-3, ω-6 PUFA” dan Kolesterol Telur dan Karkas Ayam Kampung [tesis]. Bogor: Program Pascasarjana, Institut Pertanian Bogor. Scales WE, Chensue SW, Otterness I, Kunkel SL. 1989. Regulation of monokine expresion: Prostaglandin E2 suppresses tumor necrosis factor but not interleukin-1α or β-mRNA and cell-associated bioactivity. J Leukocyte Biol 45:416-421. Schmidt EB et al. 1991. n-3 fatty acids and leukocyte chemotaxis. Effects in hyperlipidemia and dose-response studies in healthy men. Arteriosclerosis Thrombosis 11:429-435.
88
Schmidt A, Thierauch KH, Schleuning WD, Dinter H. 1995. Slice variant of human EP3 receptor for prostaglandin E2. Eur J Biochem 228:23-30. Setiabudi E. 1990. Pengaruh Waktu Penyimpanan dan Jenis Filter pada Jumlah Asam Lemak Omega-3 dalam Minyak Limbah Hasil Pengalengan dan Penepungan Ikan Lemuru [tesis]. Bogor: Program Pascasarjana, Institut Pertanian Bogor. Sijben JW, Schrama JW, Niuewland MG, Parmentier HK. 2000. Immunomodulatory effects of Indomethacin and Prostaglandin E2 on primary and secondary antibody response in growing layer hens. Poult Sci 79:949955. Sijben JW, Niuewland MG, Kemp B, Parmentier HK, Schrama JW. 2001. Interaction and antigen dependence of dietary n-3 and n-6 polyunsaturated fatty acids on antibody responsiveness in growing layer hens. Poult Sci 80 : 885-893. Smith WL, Borgeat P. 1985. The eicosanoid as prostaglandin, thromboxanes, leukotrins and hydroxyeicosanoid-acid. Di dalam: Vance DE , Vance JE, editor. Biochemistry of Lipid and Membranes. California: The Benyamin Cumming Pub. Co. Inc. Soeharsono. 1997. Pengaruh Pemberian Minyak Ikan Lemuru (Sardinella longiceps) terhadap Penampilan Tubuh dan Aktivitas Beberapa Organ Limfoid Ayam Pedaging yang Divaksin Tetelo [tesis]. Bogor: Program Pascasarjana, Institut Pertanian Bogor. Steel GD, Torrie JH. 1991. Prinsip dan Prosedur Statistika. Sumantri B, Penerjemah; Jakarta: Penerbit PT Gramedia Pustaka Utama. Terjemahan dari : Principles and Procedures of Statistics. Suprijana O. 1995. Pengaruh minyak ikan dalam diet terhadap lipoprotein plasma pada tikus. J of Padjadjaran University. 13: 29. Tizard I. 1982. Pengantar Imunologi Veteriner. Partodiredjo M dan Hardjosworo S Penerjemah; Surabaya: Penerbit Airlangga University Pr. Terjemahan dari : An Introduction to Veterinary Immunology. Wahju J. 1997. Ilmu Nutrisi Unggas. Yogyakarta : UGM Pr. Wander RC, Hall JA, Gradin JL, Du S -H, Jewe DE. 1997. The Ratio of Dietary (n-6) to (n-3) Fatty Acids Influence Immune System Function, Eicosanoid Metabolism, Lipid Peroxidation and Vitamin E Status in Aged Dogs. J Nutr 127:1198-1205. Wander RC, Du S-H, Ketchum S0, Rowe KE. 1996a. Effect of interaction of RRR α-tocopheryl acetate and fish oil on low-density-lipoprotein oxidation in postmenopausal women with and without hormon replacement therapy. Am J Clin Nutr 63:184-193.
89
Wander RC, Du S-H, Ketchum S0, Rowe KE. 1996b. α-tocopherol influences in vivo indices of lipid peroxidation in postmenopausal women given fish oil. J Nutr 126:643-652. Whelan J, Broughton KS, Kinsella JE. 1991. The comparative effects of dietary α-linolenic acid and fish oil on 4- and 5-series leukotriene formation in vivo. Lipids 26:119-126. Virella G, Kilpatrick JM, Rugeles MT, Hyman B, Rusell R. 1989. Depression of humoral respons and phagocytic function in vivo and in vitro by fish oil and eicosapentanoic acid. Clin Immunol Immunopathol 52:257-270. Zoschke DC, Messner. 1984. Suppresion of human lymphocyte mitogenesis mediated by phagocyte – released reaction oxygen species: comparative activities in normal and in chronic granulomatous disease. Clin Immunol Immunopathol 32:29-40.
90
Lampiran 1. Analisis konsentrasi asam lemak (AOCS official method Ce 1-62 1990) Sebanyak ± 25 mg sampel ditimbang secara tepat, kemudian ditambahkan standar internal (C17 : 0) sebanyak 0.5 mg. Ke dalam campuran ditambahkan 1.5 ml NaOH metanolik 0.5 N, kemudian tabung diisi dengan gas N2, ditutup rapat, dikocok dan dipanaskan dalam penangas air suhu 100 0C selama 5 menit. Setelah tabung didinginkan, ke dalam campuran ditambahkan 2 ml BF3-metanol (14% b/v), tabung diisi dengan gas N2 ditutup dan tabung divorteks selama 30 detik. Ke dalam tabung segera ditambahkan larutan NaCl jenuh sebanyak 5 ml, diisi dengan gas N2 ditutup dan dikocok. Lapisan heksana dipisahkan dan dipindahkan ke dalam vial. Fase metanol/air diekstrak kembali dengan 1 ml heksana dan hasil ekstraksi digabung serta dilakukan penyaringan dengan Na2SO4 anhidrid.
Sampel methil
ester asam lemak dikonsentratkan dengan hembusan gas N2 hingga volume ± 1 ml. Sampel siap diinjeksikanke alat kromatografi gas. Kondisi pemisahan asam lemak adalah suhu injektor 250 0C, suhu detektor 260 0C, suhu kolom awal 140 0C yang dipertahankan selama 6menit, peningkatan suhu kolom kemudian adalah 3 0C per menit sehingga suhu akhir 230 0C dan dipertahankan selama 20 menit.
Gas
2
pembawa digunakan helium dengan tekanan 1 kg/cm , sedangkan tekanan gas Hidrogen dan udara masing-masing adalah 0.5 kg/cm2.
Identifikasi dilakukan
dengan menggunakan standar methil ester asam lemak dan kuantitasi dilakukan dengan perbandingan standar internal.
91
Lampiran 2. Koefisien variasi bobot awal ayam broiler umur 1 hari (DOC) penelitian tahap I Perlakuan R1
R2
R3
R4
R5
R6
R7
R8
R9
Ulangan 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 Jumlah total Rataan STDEV KV
1
2 47 40 46 42 41 41 46 39 42 48 42 42 44 41 50 47 41 43 45 43 42 51 46 45 43 44 46 38 42 46 40 50 45 45 39 48
3 44 42 41 45 42 42 39 44 40 45 43 41 42 50 42 44 42 46 52 41 41 43 43 40 45 45 47 43 42 42 46 41 42 42 42 48
41 45 43 46 43 39 40 42 41 40 39 49 44 43 44 43 44 38 47 43 46 42 39 43 43 40 48 45 47 42 44 40 43 43 42 48 9534 44.13889 3.089241 6.99891
4
5 40 47 44 41 40 47 49 44 39 47 45 46 49 39 42 42 47 46 46 46 40 47 48 51 41 39 45 45 42 42 41 47 43 43 42 45
%
6 46 46 45 45 44 40 45 45 47 46 50 51 49 44 45 44 48 42 42 46 44 46 42 50 45 47 42 46 46 46 45 47 49 49 44 37
45 40 48 45 45 46 48 46 48 48 46 46 50 48 44 39 48 40 43 45 40 45 52 46 45 46 43 49 46 46 44 40 44 44 46 40
92
Lampiran 3. Analisis statistika pengaruh tingkat penggunaan minyak ikan dan suplementasi vitamin E dalam ransum terhadap pertambahan bobot badan ayam broiler Ulangan
Rataan Total
R1 2118 1764 1806 1875 1891 7563
R2 2007 1947 1741 1880 1894 7576
R3 1947 1606 1923 1867 1836 7343
R4 1848 1708 1888 1857 1825 7301
Faktor 0 100 200
Vit. E Total Total 2
Sumber
db
Perlakuan M. Ikan Vit. E MXE Galat Total
8 2 2 4 27 35
0 7562.70 7301.00 7682.23 22545.93 508319110
JK 91100.83 25093.0183 47498.8846 18508.92 502181.17 593282.00
R5 1805 1746 1810 1624 1746 6985
M. Ikan 3 7575.58 6985.23 7296.23 21857.05 477730635
KT
R6 1741 1773 1944 1745 1801 7202
R7 1998 1642 1899 2144 1921 7682
6 7342.83 7202.27 7346.95 21892.05 479261853
Fhit
11387.6034 0.6122597 12546.5092 0.6745688 23749.4423 1.27689961 4627.23113 0.2487852 18599.3026
R8 1687 1800 1930 1879 1824 7296
R9 1990 1959 1685 1713 1837 7347
Total 22481.12 21488.50 22325.42 66295.03
F0.05 2.31 3.35 3.35 2.73
505400607 461755632 498424229
93
Lampiran 4. Analisis statistika pengaruh tingkat penggunaan minyak ikan dan suplementasi vitamin E dalam ransum terhadap konsumsi ransum ayam broiler Ulangan
R1 4176 3472 3724 3777 3787 15149
Rataan Total
R2 R3 R4 R5 R6 R7 R8 R9 4048 3564 4169 3959 3941 3818 3929 3836 3606 3319 4062 3928 3720 3552 3783 3307 4252 3181 4101 3776 3652 3428 3675 3336 4234 3372 3814 3424 3748 3555 3663 3541 4035 3359 4036 3772 3765 3588 3763 3505 16141 13436 16146 15087 15061 14353 15050 14019
Faktor
Vit. E
0 100 200 Total Total 2
Sumber
M. Ikan 0 3 6 15148.90 16140.53 13435.92 16145.89 15086.78 15060.67 14352.75 15050.00 14019.21 45647.54 46277.31 42515.79 2083697807 2141589009 1807592305
db
Perlakuan M. Ikan Vit. E MXE Galat Total
8 2 2 4 27 35
JK
KT
Fhit
F0.05
1610557.07 676485.3264 344506.1693 589565.58 1205001.06 2815558.13
201319.6343 338242.6632 172253.0846 147391.3948 44629.66889
4.510892403 7.578874583 3.85960929 3.302542869
2.31 3.35 3.35 2.73
Perlakuan Konsumsi (g) R4 R2 R1 R5 R6 R8 R7 R9 R3
4036 4035 3787 3772 3765 3763 3588 3505 3359
Total 44725.34 2000356436 46293.33 2143072711 43421.96 1885466224 134440.63
SSR LSR 0.05 0.05
Perbedaan antar perlakuan
1 249 265 271 274 448 532 677
248 263 16 270 22 273 25 447 199 530 282 676 428
7 9 184 267 413
3 177 174 260 258 406 404
83 229
2.91 3.05 3.14 3.21 3.27 3.30 3.34 146 3.36
306.85 322.17 331.15 338.54 344.88 348.57 352.27 354.38
94
Lampiran 5. Analisis statistika pengaruh tingkat penggunaan minyak ikan dan suplementasi vitamin E dalam ransum terhadap konversi ransum ayam broiler Ulangan 1 2 3 4 Rataan Total
R1 1.97 1.97 2.06 2.01 2.00 8.02
R2 2.02 1.85 2.44 2.25 2.14 8.56
R3 1.83 2.07 1.65 1.81 1.84 7.36
Faktor
Vit. E
0 100 200 Total Total 2
Sumber Perlakuan M E ME Galat Total
0 8.02 8.86 7.54 24.41 596.0845
db 8 2 2 4 27 35
R4 2.26 2.38 2.17 2.05 2.22 8.86
M. Ikan 3 8.56 8.64 8.28 25.48 649.4374
R5 2.19 2.25 2.09 2.11 2.16 8.64
R6 2.26 2.10 1.88 2.15 2.10 8.39
6 7.36 8.39 7.66 23.41 547.9721
R7 1.91 2.16 1.80 1.66 1.88 7.54
Total 23.94 25.89 23.48 73.31
JK
KT
Fhit
F0.05
0.56 0.179501 0.271437 0.11 0.77 1.33
0.070151 0.089751 0.135718 0.027568 0.028568
2.455567 3.141612 4.750662 0.964998
2.31 3.35 3.35 2.73
Signifikansi
M. Ikan
FC
a ab b
3% 0% 6%
2.12 2.03 1.95
Signifikansi
Vit. E
FC
a b b
100 ppm 0 ppm 200 ppm
2.16 1.99 1.96
Perbedaan Antar Perlakuan
0.09 0.17
0.08
Perbedaan Antar Perlakuan
0.16 0.20
0.04
R8 2.33 2.10 1.90 1.95 2.07 8.28
R9 1.93 1.69 1.98 2.07 1.92 7.66
572.991 670.0912 551.515
SSR 0.05 2.905 3.05 SSR 0.05 2.905 3.05
LSR 0.05
0.141742 0.148817 LSR 0.05
0.141742 0.148817
95
Lampiran 6. Analisis statistika pengaruh tingkat penggunaan minyak ikan dan suplementasi vitamin E dalam ransum terhadap titer antibodi setelah vaksin ND pertama R1 6 4 6 6 6 5 5 6 7 4 8 3 5.5 66
R2 6 7 6 6 5 6 7 6 6 5 6 6 6 72
R3
R4
6 5 8 5 5 5 7 6 4 4 6 6 5.58 67
5 7 6 6 5 6 5 5 6 6 6 5 5.67 68
R5
Faktor
Vit. E
Sumber Perlakuan M E ME Galat Total
R6
7 5 7 6 5 6 6 6 6 5 5 5 5.75 69
5 6 4 6 7 6 5 5 4 5 4 7 5.33 64
0
0 66
M. Ikan 3 72
100 200 Total Total 2
68 71 205 42025
69 67 208 43264
db 8 2 2 4 99 107
JK 5.185185 0.351852 1.12963 3.703704 107.33 112.52
KT 0.648148 0.175926 0.564815 0.925926 1.084175
R7
R8
R9
6 6 6 5 9 4 5 6 6 6 5 7 5.92 71
6 4 6 6 5 6 5 7 5 6 5 6 5.58 67
6 67
Total 205
42025
64 72 203 41209
201 210 616
40401 44100 379456
Fhit 0.597826 0.162267 0.520963 0.854037
F0.05 2.056 3.106 3.106 2.486
5 5 6 7 6 5 4 7 9 6 5 7 6 72
96
Lampiran 7. Analisis statistika pengaruh tingkat penggunaan minyak ikan dan suplementasi vitamin E dalam ransum terhadap titer antibodi setelah vaksin ND kedua
R1
R2
R3
R4
R5
R6
R7
R8
R9
7 5 6
5 7 6
8 8 8
8 9 5
7 7 7
7 8 7
8 6 6
8 8 7
8 8 8
7 7 7 6 4 7 5 6 6 6.08 73
7 8 7 5 6 4 7 8 7 6.42 77
6 7 8 7 6 8 6 7 7 7.17 86
10 9 8 8 8 8 9 10 9 8.42 101
7 7 6 8 8 8 10 8 8 7.58 91
9 8 9 6 8 6 7 10 10 7.92 95
9 7 7 7 7 6 7 8 6 7.00 84
8 8 7 7 8 8 8 8 7 7.67 92
10 7 10 11 11 11 9 8 8 9.08 109
Faktor 0 Vit. E
Sumber Perlakuan M E ME Galat Total
0 100 200 Total Total 2 db 8 2 2 4 99 107
73 101 84 258 66564
M. Ikan 3 77 91 92 260 67600
JK 85.12963 17.85185 46.35185 20.92593 121.83 206.96
KT 10.6412 8.925926 23.17593 5.231481 1.23064
6 86 95 109 290 84100
Total 236 287 285 808
Fhit 8.646888 7.253078 18.83242 4.251026
F0.05 2.056 3.106 3.106 2.486
55696 82369 81225 652864
97
Perlakuan R9 R4 R6 R8 R5 R3 R7 R2 R1
Perbedaan Rataan Antar Perlakuan 9.08 8.42 7.92 7.67 7.58 7.17 7.00 6.42 6.08
0.67 1.17 1.42 1.50 1.92 2.08 2.67 3.00
0.50 0.75 0.83 1.25 1.42 2.00 2.33
0.25 0.33 0.75 0.92 1.50 1.83
0.08 0.50 0.67 1.25 1.58
0.42 0.58 1.17 1.50
SSR 0.05
0.17 0.75 1.08
0.58 0.92
0.33
2.80 2.95 3.05 3.12 3.18 3.22 3.26 3.29
LSR 0.05
Signi fikansi
0.90 0.94 0.98 1.00 1.02 1.03 1.04 1.05
a ab bc bc bc cd cde de e
98
Lampiran 8. Analisis statistika pengaruh tingkat penggunaan minyak ikan dan suplementasi vitamin E dalam ransum terhadap titer antibodi setelah vaksin IBD R1
R2
R3
R4
R5
R6
R7
R8
R9
0 3 3 3 2 2 2 2 2 2.11 19
1 3 2 2 3 2 2 2
1 3 1 2 2 2 2 3 4 2.22 20
2 4 2 2 4 2 2 2 2 2.44 22
2 3 3 3 2 2 3 3
3 3 4 3 2 3 3 2
2.63 21
2.88 23
3 3 3 3 2 3 4 3 2 2.89 26
3 1 2 3 2 4 2 3 3 2.56 23
2 2 2 3 3 4 3 3 2 2.67 24
2.13 17
Faktor 0 Vit. E
Sumber Perlakuan M E* ME Galat Total Vitamin E 200 100 0
0 100 200 Total Total 2 db 8 2 2 4 69 77
19 22 26 67 4489 JK 6.097222 0.253105 4.725755 1.118362 41.40 47.50
M. Ikan 3 17 21 23 61 3721 KT 0.762152778 0.126552707 2.362877493 0.279590456 0.600040258
Perbedaan Rataan Antar Perlakuan 2.703704 2.648148 2.225694
0.055556 0.478009
0.422453704
6 20 23 24 67 4489 Fhit 1.270169 0.210907 3.937865 0.465953
Total 56 66 73
3136 4356 5329
F0.05 2.088 3.138 3.138 2.518
SSR 0.05
LSR 0.05
Signifikansi
2.82325 2.971
0.428897 0.451342
a ab b
99
Lampiran 9. Analisis statistika pengaruh tingkat penggunaan minyak ikan dan suplementasi vitamin E dalam ransum terhadap eosinofil R1
R2
R3
R4
R5
R6
R7
R8
R9
2 0 4 8 0 2 5 2 2.88
3 1 2 5 3 2 0 2 2.25
1 1 1 0 0 2 0 3 1.00
0 3 3 1 0 0 0 0 0.88
0 3 1 0 5 0 0 0 1.13
0 1 1 0 0 1 0 1 0.50
1 0 0 2 0 0 0 0 0.38
3 2 1 3 0 3 3 0 1.88
2 1 4 1 0 0 0 0 1.00
R1
R2
R3
R4
R5
R6
R7
R8
R9
8.13 0.00 11.54 16.43 0.00 8.13 12.92 8.13 8.16 65.28
9.97 5.74 8.13 12.92 9.97 8.13 0.00 8.13 7.87 63.00
5.74 5.74 5.74 0.00 0.00 8.13 0.00 9.97 4.42 35.32
0.00 9.97 9.97 5.74 0.00 0.00 0.00 0.00 3.21 25.69
0.00 9.97 5.74 0.00 12.92 0.00 0.00 0.00 3.58 28.63
0.00 5.74 5.74 0.00 0.00 5.74 0.00 5.74 2.87 22.96
5.74 0.00 0.00 8.13 0.00 0.00 0.00 0.00 1.73 13.87
9.97 8.13 5.74 9.97 0.00 9.97 9.97 0.00 6.72 53.77
8.13 5.74 11.54 5.74 0.00 0.00 0.00 0.00 3.89 31.15
Faktor 0 100 200
Vit. E Total
Sumber
a b b
M. Ikan 3 62.9988875 28.6343587 53.76616 145.399406 21140.9873
6 35.3218356 22.9566819 31.1454023 89.4239198 7996.63744
Total 163.598896 77.2786546 98.7808352
JK
KT
Fhit
F0.05
339.883402 69.6697849 168.262622 101.950995 1211.75541 1551.63882
42.4854252 34.8348925 84.1313109 25.4877487 19.2342129
2.20884657 1.81109009 4.3740449 1.32512564
db
Perlakuan M. Ikan Vit. E MXE Galat Total Signifikansi
0 65.2781727 25.6876141 13.8692728 104.83506 10990.3897
8 2 2 4 63 71 Vit. E 0 200 100
Eisonofil 6.82 4.12 3.22
Perbedaan Antar Perlakuan 2.70 3.60
0.90
26764.5987 5971.99046 9757.6534
2.096 3.146 3.146 2.526
SSR 0.05 2.83 2.98
LSR 0.05 2.53 2.67
100
Lampiran 10. Analisis statistika pengaruh tingkat penggunaan minyak ikan dan suplementasi vitamin E dalam ransum terhadap heterofil R1
R2
R3
R4
R5
R6
R7
R8
R9
9 21 51 36 13 18 13 29 23.75
25 18 14 56 8 41 10 54 28.25
18 16 23 17 23 12 14 43 20.75
19 17 14 10 12 52 18 13 19.38
16 10 46 58 7 10 15 42 25.50
15 15 4 14 18 20 17 5 13.50
27 21 19 17 29 16 14 38 22.63
7 12 28 25 10 36 2 30 18.75
1 5 14 21 10 28 13 22 14.25
R1
R2
R3
R4
R5
R6
R7
R8
R9
17.46 27.27 45.57 36.87 21.13 25.10 21.13 32.58 28.39 227.13
30.00 25.10 21.97 48.45 16.43 39.82 18.43 47.29 30.94 247.50
25.10 23.58 28.66 24.35 28.66 20.27 21.97 40.98 26.70 213.57
25.84 24.35 21.97 18.43 20.27 46.15 25.10 21.13 25.41 203.25
23.58 18.43 42.71 49.60 15.34 18.43 22.79 40.40 28.91 231.28
22.79 22.79 11.54 21.97 25.10 26.57 24.35 12.92 21.00 168.02
31.31 27.27 25.84 24.35 32.58 23.58 21.97 38.06 28.12 224.96
15.34 20.27 31.95 30.00 18.43 36.87 8.13 33.21 24.28 194.20
5.74 12.92 21.97 27.27 18.43 31.95 21.13 27.97 20.92 167.40
0 100 200 Total
0 227.1306 203.2522 224.9635 655.3464 429478.9
Faktor
Vit. E
M. Ikan 3 247.4972 231.282 194.2035 672.9827 452905.7
6 213.5654 168.0229 167.397 548.9853 301384.9
Total 688.1932 602.5571 586.5641
473609.9 363075.1 344057.4
101
Lampiran 11. Analisis statistika pengaruh tingkat penggunaan minyak ikan dan suplementasi vitamin E dalam ransum terhadap limfosit R1
R2
R3
R4
R5
R6
R7
R8
R9
89 79 45 56 87 80 82 69 73.375
72 81 84 39 89 57 90 44 69.5
81 83 76 83 77 86 86 54 78.25
81 80 83 89 88 48 82 87 79.75
84 87 53 42 88 90 85 58 73.375
85 84 95 86 82 79 83 94 86
72 79 81 81 71 84 86 62 77
90 86 71 72 90 61 95 70 79.375
97 94 82 78 90 72 87 78 84.75
R1
R2
R3
R4
R5
R6
R7
R8
R9
70.63 62.73 42.13 48.45 68.87 63.43 64.90 56.17 59.66 477.30
58.05 64.16 66.42 38.65 70.63 49.02 71.57 41.55 57.51 460.05
64.16 65.65 60.67 65.65 61.34 68.03 68.03 47.29 62.60 500.81
64.16 63.43 65.65 70.63 69.73 43.85 64.90 68.87 63.90 511.22
66.42 68.87 46.72 40.40 69.73 71.57 67.21 49.60 60.06 480.52
67.21 66.42 77.08 68.03 64.90 62.73 65.65 75.82 68.48 547.83
58.05 62.73 64.16 64.16 57.42 66.42 68.03 51.94 61.61 492.90
71.57 68.03 57.42 58.05 71.57 51.35 77.08 56.79 63.98 511.85
80.03 75.82 64.90 62.03 71.57 58.05 68.87 62.03 67.91 543.28
Faktor 0 100 200
Vit. E Total
Sumber Perlakuan M. Ikan Vit. E MXE Galat Total M. Ikan 6 0 3
db 8 2 2 4 63 71 Lymfosit 66.33 61.73 60.52
0 477.29543 511.22074 492.90297 1481.4191 2194602.7
M. Ikan 3 460.05052 480.51809 511.84922 1452.4178 2109517.5
6 500.81465 547.8339 543.28137 1591.9299 2534240.8
Total 1438.1606 1539.5727 1548.0336
JK
KT
Fhit
F0.05
861.99142 451.62956 311.50094 98.860915 5157.7777 6019.7691
107.74893 225.81478 155.75047 24.715229 81.869487
1.3161061 2.7582289 1.9024239 0.3018857
Perbedaan Antar Perlakuan 4.60 5.81
1.21
2068305.9 2370284.2 2396407.9
2.096 3.146 3.146 2.526
SSR 0.05 2.83 2.98
LSR 0.05
Signifikansi
5.22 5.50
a ab b
102
Lampiran 12. Koefisien variasi bobot awal ayam broiler umur 1 hari (DOC) penelitian tahap II Kode
1
2
3
4
5
6
7
8
9
10
1.1. 1.2. 1.3 1.4 2.1 2.2 2.3 2.4 3.1 3.2 3.3 3.4 4.1 4.2 4.3 4.4 5.1 5.2 5.3
47 49 48 50 50 47 40 50 51 50 53 54 48 44 42 54 52 48 49
50 51 51 48 47 47 43 48 49 45 49 49 43 48 53 45 42 50 53
50 49 51 52 52 48 40 48 53 51 44 48 48 50 55 48 55 47 46
50 51 49 42 52 52 46 36 48 47 51 55 53 48 44 48 50 48 54
52 47 49 49 47 49 45 51 50 51 46 54 51 49 54 50 59 51 51
50 39 51 49 49 51 55 44 48 54 51 52 53 46 50 48 47 52 48
53 48 45 52 50 48 48 50 55 52 55 44 47 52 52 61 49 52 45
49 52 49 50 53 47 49 49 44 50 50 51 50 46 52 51 52 52 45
42 47 56 50 40 50 48 50 54 48 48 52 49 41 53 43 48 48 53
53 51 48 48 54 53 40 56 54 51 52 53 46 46 47 47 42 50 50
6.1 6.2 6.3 6.4 7.1 7.2 7.3 7.4 8.1 8.2 8.3
51 47 48 46 55 49 53 50 51 50 50
52 53 48 53 47 55 49 53 55 55 56
50 52 53 54 51 51 56 46 51 47 51
55 48 54 53 50 52 45 58 49 52 50
51 45 56 57 55 45 56 49 48 49 56
48 50 43 43 48 45 50 51 52 50 45
50 52 43 53 56 50 52 54 49 51 51
49 51 48 52 51 50 46 52 44 55 52
48 48 51 56 54 54 48 57 53 51 47
50 50 49 48 46 57 52 50 44 46 45
Jumlah Total Rataan STDEV KV
14909 49.7 3.72 7.485
%
103
Lampiran 13. Analisis statistika pengaruh perlakuan terhadap konsumsi ransum sebelum dan sesudah uji tantang virus IBD atau ND
Total
1
2
3
4
5
6
7
8
1187.90 1119.20 1192.08 1169.97 1167.29 4669.14
1260.87 1190.76 1168.96 1130.42 1187.75 4751.00
1180.45 1119.04 1140.23 1085.26 1131.24 4524.97
1063.93 1116.91 1028.96
1135.61 1268.08 1112.31 1195.83 1177.96 4711.84
1253.74 1133.48 1183.07 1181.01 1187.83 4751.31
1147.62 1183.31 1109.68 1153.48 1148.52 4594.09
1036.94 1132.52 1086.76
Sumber
db
JK
Perlakuan Galat Total
7 22 29
Perla kuan 6 2 4 1 7 3 8 5
1069.93 3209.80 KT
48877.18993 50269.52 99146.71
Fhit
F0.05
6982.455705 3.055808578 2284.978109
2.46
Perbedaan Antar Perlakuan 1187.83 1187.75 1177.96 1167.29 1148.52 1131.24 1085.41 1069.93
0.08 9.87 20.54 39.30 56.58 102.42 117.89
9.79 20.46 39.23 56.51 102.34 117.82
10.67 29.44 46.72 92.55 108.03
18.76 36.04 81.88 97.35
SSR 0.05
17.28 63.11 78.59
45.84 61.31
1085.41 3256.22
15.47
2.93 3.08 3.17 3.24 3.29 3.32 3.35
LSR 0.05
Signi fikansi
70.03 73.61 75.77 77.44 78.63 79.35 80.07
a a a a a ab b b
Umur 3 – 4 minggu (Sesudah ditantang virus IBD)
1 2 3 4 Total
1
2
3
827.89 795.56 767.78 769.22 790.11 3160.44
856.00 757.89 769.00 738.56 780.36 3121.44
913.38 670.29 854.89 701.89 785.11 3140.44
Sumber Perlakuan Galat Total
db 7 22 29
4 853.78 826.44 833.22 837.81 2513.44
5
6
7
8
907.33 778.89 871.67 874.33 858.06 3432.22
852.56 609.44 785.00 779.78 756.69 3026.78
798.22 785.00 788.56 825.25 799.26 3197.03
747.67 704.13 806.44 752.75 2258.24
JK
KT
Fhit
F0.05
34045.51867 99951.18 133996.70
4863.645524 4543.235326
1.070524676
2.46
104
Umur 4 – 5 minggu (Sesudah uji tantang virus IBD atan ND)
1 2 3 4 Total
1
2
3
4
5
6
7
857.66 891.17 895.35 915.60 889.94 3559.78
875.46 903.45 878.53 917.32 893.69 3574.75
888.47 1102.58 866.37 757.85 903.82 3615.27
899.52 873.00 921.86
755.76 836.29 891.79 744.71 807.14 3228.55
738.20 1037.88 903.20 921.99 900.32 3601.27
927.39 901.24 844.27 865.39 884.57 3538.29
Sumber
db
Perlakuan Galat Total
7 22 29
898.13 2694.39
8 840.22 795.41 915.85 850.49 2551.48
JK
KT
Fhit
F0.05
29938.81077 138343.40 168282.21
4276.972967 6288.336176
0.680143817
2.46
Umur 5 – 6 minggu (Sesudah uji tantang virus IBD atau ND)
1 2 3 4 Total
1
2
3
4
5
6
7
8
1050.68 978.19 969.80 984.91 995.90 3983.58
1153.86 992.75 986.59 985.19 1029.60 4118.38
1065.02 989.89 979.17 909.32 985.85 3943.40
999.46 1126.08 939.85
1014.02 756.10 1015.84 954.40 935.09 3740.36
1048.86 1144.26 1033.89 1053.48 1070.12 4280.49
1050.68 1040.05 1049.28 1000.58 1035.15 4140.59
1114.32 987.15 1156.30
Sumber
db
Perlakuan Galat Total
7 22 29
1021.80 3065.39
JK
KT
Fhit
F0.05
60046.44679 124883.70 184930.15
8578.063828 5676.532004
1.511145154
2.46
1085.92 3257.77
Umur 3 – 6 minggu (Sesudah uji tantang virus IBD atau ND)
1 2 3 4 Total
1
2
3
4
5
6
7
8
2736.23 2664.92 2632.92 2669.73 2675.95 10703.80
3007.78 2654.08 2634.12 2641.07 2734.26 10937.05
2991.16 3126.46 2700.43 2369.06 2796.78 11187.10
2752.76 2947.65 2694.93
2677.11 2371.29 2779.29 2573.45 2600.28 10401.13
2639.62 2791.58 2722.09 2755.25 2727.14 10908.54
2776.29 2726.29 2682.11 2691.22 2718.98 10875.91
2702.21 2597.96 3006.71
Sumber Perlakuan Galat Total
db 7 22 29
2798.45 8395.35
JK
KT
Fhit
F0.05
114896.0808 678785.42 793681.50
16413.72583 30853.88277
0.531982505
2.46
2768.96 8306.87
105
Lampiran 14. Analisis statistika pengaruh perlakuan terhadap pertambahan bobot badan sebelum dan sesudah uji tantang virus IBD atau ND Umur 1 – 3 minggu (Sebelum uji tantang virus IBD atau ND)
Total
1
2
3
4
5
6
7
8
634.62 457.82 454.86 491.33 509.66 2038.63
565.10 581.80 548.71 475.36 542.74 2170.97
475.65 359.81 546.32 449.47 457.81 1831.25
510.42 551.88 550.24 541.06 538.40 2153.60
441.62 505.87 450.82
562.49 450.03 496.26 474.83 495.90 1983.60
475.03 477.53 403.30 387.22 435.77 1743.09
367.73 542.40 579.59
Sumber
db
Perlakuan Galat Total
7 22 29
466.10 1398.31
JK
KT
Fhit
F0.05
39533.7669 88866.07 128399.84
5647.680992 4039.366925
1.398159934
2.46
496.57 1489.72
Umur 3 – 4 minggu (Sesudah uji tantang virus IBD)
Total
1
2
3
4
434.53 336.90 403.11 353.11 381.91 1527.65
407.13 397.22 386.56 372.67 390.89 1563.57
462.13 286.43 463.56 281.44 373.39 1493.55
424.56 414.67 433.89 420.37 423.37 1693.49
Sumber
db
Perlakuan Galat Total
7 22 29
5 452.74 439.77 478.32 456.94 1370.83
6
7
434.11 320.38 356.33 340.44 362.82 1451.26
324.78 371.89 336.78 344.90 344.59 1378.35
JK
KT
F
40812.4854 55427.40 96239.88
5830.355051 2519.427237
2.314158935
8 327.33 285.75 402.33 338.47 1015.42
F0.05 2.46
Umur 4 – 5 minggu (Sesudah uji tantang virus IBD atau ND)
Total
1
2
3
4
5
6
7
515.13 522.00 488.08 441.06 491.57 1966.26
560.66 448.65 429.58 490.90 482.45 1929.80
500.77 427.02 308.72 326.39 390.73 1562.90
425.72 524.95 515.26 484.65 487.65 1950.58
350.52 469.36 487.12
470.88 474.29 671.74 508.97 531.47 2125.87
476.64 480.40 456.35 467.88 470.32 1881.26
435.67 819.88
8 358.46 481.82 435.63 425.30 1275.91
106
Sumber
db
Perlakuan Galat Total
7 22 29
JK
KT
Fhit
F0.05
53599.3226 90604.44 144203.77
7657.046079 4118.383775
1.859235685
2.46
Umur 5 – 6 minggu (Sesudah uji tantang virus IBD atau ND)
Total
1
2
3
4
5
6
7
8
476.41 409.30 397.54 439.21 430.62 1722.46
634.55 468.98 489.75 504.45 524.43 2097.73
367.46 372.83 305.79 160.50 301.64 1206.58
524.75 293.51 269.20 358.49 361.49 1445.95
264.74 331.78 332.45
470.70 529.21 302.95 429.08 432.99 1731.94
447.39 420.66 460.73 443.86 443.16 1772.64
386.21 457.60 482.31
Sumber
db
Perlakuan Galat Total
7 22 29
Perla kuan 2 7 8 6 1 4 5 3
309.66 928.97
JK
KT
Fhit
F0.05
157493.907 126639.49 284133.40
22499.12957 5756.340476
3.908582139
2.46
Perbedaan Antar Perlakuan 524.43 443.16 81.27 442.04 82.39 1.12 432.99 91.45 10.18 430.62 93.82 12.54 361.49 162.94 81.67 309.66 214.78 133.50 301.64 222.79 141.52
9.05 11.42 80.55 132.38 140.39
SSR 0.05
2.93 3.08 3.17 3.24 3.29 3.32 3.35
2.37 71.50 69.13 123.33 120.96 51.83 131.34 128.97 59.84 8.01
442.04 1326.11
LSR 0.05
Signi fikansi
111.15 116.84 120.25 122.91 124.81 125.95 127.08
a ab ab abc abc bcd cd d
Umur 3 – 6 minggu (Sesudah uji tantang virus IBD atan ND)
Total
1
2
3
4
5
6
7
8
1426.06 1268.21 1288.73 1233.38 1304.10 5216.38
1602.33 1314.86 1305.89 1368.02 1397.77 5591.10
1330.35 1086.29 1078.06 768.33 1065.76 4263.03
1374.03 1229.13 1217.35 1269.50 1272.50 5090.01
1173.52 1174.72 1258.53
1375.68 1323.88 1331.02 1278.50 1327.27 5309.07
1248.81 1272.95 1253.86 1256.63 1258.06 5032.25
1072.00 1225.17 1320.28
Sumber Perlakuan Galat Total
db
JK 7 22 29
274658.105 298728.96 573387.07
1202.26 2348.24
KT 39236.87211 13578.58915
Fhit 2.889613322
1205.81 3617.44 F0.05
2.46
107
Perla kuan 2 6 1 4 7 8 5 3
Perbedaan Antar Perlakuan 1397.77 1327.27 1304.10 1272.50 1258.06 1205.81 1202.26 1065.76
70.51 93.68 23.17 125.27 54.76 31.59 139.71 69.20 46.03 14.44 191.96 121.45 98.28 66.69 52.25 195.51 125.01 101.83 70.24 55.80 3.55 332.02 261.51 238.34 206.75 192.31 140.06 136.50
SSR 0.05 2.93 3.08 3.17 3.24 3.29 3.32 3.35
LSR 0.05 170.71 179.45 184.70 188.77 191.69 193.44 195.18
Signifi kansi a ab ab ab abc bc bc c
108
Lampiran 15. Analisis statistika pengaruh perlakuan terhadap konversi ransum sebelum dan sesudah uji tantang virus IBD atau ND Umur 1 – 3 minggu (Sebelum uji tantang virus IBD atau ND) 1 1 2 3 4 Total
2
1.74 2.21 2.36 2.17 2.12 8.47
Sumber
3
2.05 1.89 1.97 2.16 2.02 8.07
4
2.24 2.73 1.91 2.17 2.26 9.05
db
Perlakuan Galat Total
7 22 29
2.17 2.01 2.06 2.08 6.23
5
6
2.03 2.12 1.85 2.02 2.01 8.03
2.05 2.27 2.17 2.24 2.18 8.73
7
8
2.18 2.24 2.44 2.63 2.37 9.49
2.48 1.91 1.73 2.04 6.12
JK
KT
Fhit
F0.05
0.460546527 1.13 1.59
0.065792361 0.05120574
1.284862997
2.46
Umur 3 – 4 minggu (Sesudah uji tantang virus IBD) 1 1 2 3 4 Total
1.91 2.36 1.90 2.18 2.09 8.35
Sumber
2
3
2.10 1.91 1.99 1.98 1.995 7.98
1.98 2.34 1.84 2.49 2.16 8.65
db
Perlakuan Galat Total
7 22 29
4 2.01 2.01 1.92 1.98 5.94
5
6
7
2.01 1.69 1.99 1.83 1.88 7.52
1.96 1.90 2.20 2.29 2.089915 8.36
8
2.46 2.11 2.34 2.39 2.326 9.30
2.28 2.46 2.00 2.25 6.75
JK
KT
Fhit
F0.05
0.568957232 0.80 1.37
0.081279605 0.036297239
2.239277886
2.46
Umur 4 – 5 minggu (Sesudah uji tantang virus IBD atau ND)
1 2 3 4 Total
1
2
3
4
5
6
7
8
1.66 1.71 1.83 2.08 1.82 7.28
1.78 2.01 2.05 1.87 1.93 7.71
2.02 3.43 2.81 2.32 2.65 10.58
2.12 1.90 1.79
1.71 2.39 1.91 1.53 1.89 7.55
1.57 2.19 1.34 1.81 1.73 6.91
1.95 2.11 2.34 2.39 2.20 8.79
2.34 1.88 2.40
1.94 5.81
2.21 6.62
109
Sumber
db
Perlakuan Galat Total
7 22 29
Perla kuan
JK
KT
Fhit
F0.05
2.410359715 2.44 4.85
0.344337102 0.110824128
3.107058981
2.46
Perbedaan Antar Perlakuan
3 8 7 4 2 5 1 6
2.65 2.21 2.20 1.94 1.93 1.89 1.82 1.73
0.44 0.45 0.71 0.72 0.76 0.83 0.92
0.01 0.27 0.28 0.32 0.39 0.48
0.26 0.27 0.31 0.38 0.47
SSR 0.05
0.01 0.05 0.12 0.21
0.04 0.11 0.20
0.07 0.16
0.09
LSR 0.05
2.93 3.08 3.17 3.24 3.29 3.32 3.35
Signifi kansi
0.49 0.51 0.53 0.54 0.55 0.55 0.56
a ab ab b b b b b
Umur 5 – 6 minggu (Sesudah uji tantang virus IBD atau ND)
1 2 3 4 Total
1
2
3
4
5
6
7
2.21 2.39 2.44 2.24 2.32 9.28
1.82 2.12 2.01 1.95 1.98 7.90
2.90 2.66 3.20 5.67 3.61 14.42
1.91 3.85 3.50
3.00 2.99 3.04 3.24 3.07 12.27
2.23 2.16 3.41 2.46 2.56 10.26
2.35 2.47 2.28 2.25 2.338 9.35
Sumber
db
3.09 9.26
Fhit
F0.05
7.670209117 9.39 17.06
1.09574416 0.426941495
2.566497217
2.46
Perla kuan
Perbedaan Antar Perlakuan 0.52 0.54 1.04 1.13 1.27 1.29 1.63
2.48 7.44
KT
7 22 29
3.61 3.09 3.07 2.56 2.48 2.34 2.32 1.98
2.89 2.16 2.40
JK
Perlakuan Galat Total
3 4 5 6 8 7 1 2
8
0.02 0.52 0.61 0.75 0.77 1.11
0.50 0.59 0.73 0.75 1.09
SSR 0.05
0.08 0.23 0.25 0.59
0.14 0.16 0.50
0.02 0.36
0.34
2.93 3.08 3.17 3.24 3.29 3.32 3.35
LSR 0.05 0.96 1.01 1.04 1.06 1.07 1.08 1.09
Signifi kansi a ab ab bc bc bc bc c
110
Umur 3 – 6 minggu (Sesudah uji tantang virus IBD atau ND) 1 1 2 3 4 Total
1.92 2.10 2.04 2.16 2.06 8.23
Sumber
2 1.88 2.02 2.02 1.93 1.96 7.84
4
5
2.25 2.88 2.50 3.08 2.68 10.71
2.00 2.40 2.22
2.31 2.06 2.25 2.05 2.16 8.65
db
Perlakuan Galat Total
7 22 29
Perla kuan 3 8 5 4 7 1 6 2
3
2.21 6.62
6
7
1.92 2.11 2.05 2.16 2.06 8.23
2.22 2.14 2.14 2.14 2.161 8.65
0.37 0.47 0.52 0.52 0.62 0.62 0.72
2.52 2.12 2.28 2.31 6.92
JK
KT
Fhit
F0.05
1.353673305 0.71 2.07
0.193381901 0.032454797
5.958499721
2.46
Perbedaan Antar Perlakuan 2.68 2.31 2.21 2.16 2.16 2.06 2.06 1.96
8
0.10 0.14 0.14 0.25 0.25 0.35
0.04 0.05 0.15 0.15 0.25
0.00 0.11 0.11 0.20
SSR 0.05
0.10 0.10 0.20
0.00 0.10
0.10
2.93 3.08 3.17 3.24 3.29 3.32 3.35
LSR 0.05
Signifi kansi
0.26 0.28 0.29 0.29 0.30 0.30 0.30
a b bc bc bc bc bc c
111
Lampiran 16. Analisis statistika pengaruh perlakuan terhadap berat relatif bursa Fabricius sebelum dan sesudah uji tantang virus IBD atau ND Umur 2 minggu
1 2 3 4 Total
1
2
3
4
5
6
7
8
0.0024 0.0029 0.0044 0.0021 0.0030 0.0118
0.0027 0.0024 0.0029 0.0029 0.0027 0.0109
0.0040 0.0099 0.0059 0.0018 0.0054 0.0216
0.0035 0.0035 0.0013 0.0033 0.0029 0.0117
0.0019 0.0027 0.0023
0.0014 0.0038 0.0017 0.0037 0.0026 0.0105
0.0023 0.0024 0.0026 0.0032 0.0026 0.0106
0.0011 0.0026 0.0013
Sumber
db
Perlakuan Galat Total
JK
7 22 29
0.0023 0.0068
KT
3.122E-05 0.00 0.00
4.46E-06 2.257E-06
Fhit
F0.05
1.975772
2.46
0.0017 0.0051
Umur 4 minggu (sesudah uji tantang virus IBD atau ND) 1 0.002 0.001 5E-04 7E-04 1E-03 0.004
Total Sumber
2
3
4
5
6
7
8
0.001 0.0007 0.0019 0.0005 0.001 0.0041
0.0034 0.0016 0.0032 0.0005 0.0022 0.0088
0.0028 0.0009 0.001 0.0015 0.0016 0.0062
0.0034 0.0012 0.0019
0.002 0.002 0.003 0.001 0.002 0.009
0.0022 0.0024 0.0024 0.0028 0.0025 0.0098
0.002 0.002 0.003
db
Perlakuan Galat Total
7 22 29
0.0022 0.0065
JK
KT
Fhit
F0.05
1.0191E-05 1.4085E-05 2.4276E-05
1.4559E-06 6.4022E-07
2.274082
2.46
0.002 0.007
Perlakuan
Rataan
Perbedaan Antar Perlakuan
SSR 0.05
LSR 0.05
Signi fikan
7 8 6 3 5 4 2 1
0.0025 0.0025 0.0023 0.0022 0.0022 0.0016 0.001 0.001
1E-05 0.0002 0.0003 0.0003 0.0009 0.0014 0.0015
2.93 3.08 3.17 3.24 3.29 3.32 3.35
0.001 0.001 0.001 0.001 0.001 0.001 0.001
a a ab ab ab ab b b
0.0002 0.0003 0.0003 0.0009 0.0014 0.0015
5E-05 9E-05 0.0007 0.0012 0.0013
4E-05 0.0006 0.0012 0.0012
0.0006 0.0011 0.0012
0.00054 0.00058
4E-05
112
Umur 5 minggu (sesudah uji tantang virus IBD atau ND) 1 6E-04 5E-04 7E-04 7E-04 6E-04 0.002
Total Sumber
2
3
4
5
6
7
8
0.0007 0.0008 0.0006 0.0009 0.0008 0.003
0.003 0.002 0.001 0.001 0.002 0.008
0.0016 0.0019 0.004 0.0035 0.0028 0.011
0.002 0.003 0.003
0.0005 0.0007 0.0007 0.0004 0.0005 0.0022
0.0008 0.0005 0.0009 0.0005 0.0007 0.0027
0.0008 0.0005 0.0019
db
0.002 0.007
JK
KT
Fhit
F0.05
2.0602E-05 7.2536E-06 2.7855E-05
2.9431E-06 3.2971E-07
8.926284
2.46
0.0011 0.0032
Perlakuan Galat Total
7 22 29
Perlakuan
Perbedaan antar Perlakuan
SSR 0.05
LSR 0.05
4E-04 8E-04 0.002 0.002 0.002 0.002 0.002
2.93 3.08 3.17 3.24 3.29 3.32 3.35
0.0008 0.0009 0.0009 0.0009 0.0009 0.0010 0.0010
4 5 3 8 2 7 1 6
0.003 0.002 0.002 0.001 8E-04 7E-04 6E-04 5E-04
4E-04 0.001 0.002 0.002 0.002 0.002
9E-04 0.001 0.001 0.001 0.001
3E-04 4E-04 5E-04 5E-04
8E-05 2E-04 2E-04
8E-05 1E-04
6E-05
Umur 6 minggu (sesudah uji tantang virus IBD atau ND)
Total
1
2
3
4
5
6
7
8
0.0005 0.0003 0.0008 0.0006 0.0006 0.0022
0.0005 0.0008 0.0006 0.0006 0.0006 0.0025
0.0008 0.0007 0.0015 0.0007 0.0009 0.0036
0.0005 0.0011 0.0009 0.0006 0.0008 0.0031
0.0004 0.0009 0.0003
0.0004 0.0005 0.0006 0.0007 0.0005 0.0021
0.00038 0.00038 0.00059 0.00074 0.00052 0.00209
0.0003 0.0005 0.0006
Sumber Perlakuan Galat Total
db 7 22 29
0.0005 0.0016
JK
KT
Fhit
F0.05
5.9022E-07 1.1340E-06 1.7242E-06
8.4317E-08 5.1545E-08
1.635795
2.46
0.0005 0.0015
Signi fikan si a a ab bc c c c c
113
Lampiran 17. Analisis statistika pengaruh perlakuan terhadap berat relatif limpa sebelum dan sesudah uji tantang virus IBD atau ND Umur 2 minggu
Total
1
2
3
4
5
6
7
8
0.0009 0.0011 0.0012 0.0004 0.0009 0.0035
0.0006 0.0009 0.0015 0.0004 0.0008 0.0033
0.0009 0.0018 0.0018 0.0004 0.0012 0.0048
0.001 0.0006 0.001 0.0003 0.0007 0.0029
0.0011 0.0007 0.0011
0.0003 0.0019 0.0014 0.0007 0.0011 0.0043
0.00116 0.00091 0.00113 0.00079 0.001 0.00399
0.0015 0.0007 0.0013
Sumber
db
Perlakuan Galat Total
7 22 29
0.001 0.0029
JK
KT
Fhit
F0.05
7.2196E-07 4.9673E-06 5.6893E-06
1.0314E-07 2.2579E-07
0.45679
2.46
0.0012 0.0035
Umur 4 minggu (sesudah uji tantang virus IBD atau ND)
Total
1
2
3
4
5
6
7
8
0.0015 0.0014 0.0013 0.002 0.0016 0.0062
0.0012 0.0011 0.0013 0.0018 0.0013 0.0054
0.0011 0.0011 0.0009 0.0011 0.001 0.0042
0.0013 0.0011 0.0008 0.0014 0.0012 0.0046
0.0014 0.001 0.0014
0.0011 0.0021 0.0018 0.0013 0.0015 0.0062
0.00147 0.00165 0.00135 0.0025 0.00174 0.00697
0.0015 0.002 0.0012
Sumber
db
Perlakuan Galat Total
7 22 29
0.0013 0.0039
JK
KT
Fhit
1.5115E-06 2.7516E-06 4.2631E-06
2.1593E-07 1.2507E-07
0.0015 0.0046
F0.05
1.726383
2.46
Umur 5 minggu (sesudah uji tantang virus IBD atau ND)
Total
1
2
3
4
5
6
7
8
0.0014 0.0007 0.001 0.0009 0.001 0.004
0.0011 0.001 0.0008 0.0012 0.001 0.0041
0.0028 0.0012 0.0008 0.0011 0.0015 0.0059
0.0019 0.002 0.0019 0.0024 0.002 0.0082
0.0011 0.0018 0.0019
0.0013 0.0005 0.0009 0.0014 0.001 0.0041
0.00124 0.00133 0.00129 0.00099 0.00121 0.00486
0.0018 0.0011 0.002
0.0016 0.0048
0.0016 0.0049
114
Sumber
db
JK
Perlakuan Galat Total
7 22 29
3.8218E-06 4.2273E-06 8.0491E-06
Per lakuan
Rataan
4 8 5 3 7 6 2 1
0.002 0.0016 0.0016 0.0015 0.0012 0.001 0.001 0.001
KT
Fhit
5.4597E-07 1.9215E-07
F0.05
2.841351
2.46
Perbedaan Antar Perlakuan
SSR 0.05
0.0004 0.0005 0.0006 0.0008 0.001 0.001 0.0011
2.93 3.08 3.17 3.24 3.29 3.32 3.35
3E-05 0.0001 0.0004 0.0006 0.0006 0.0006
0.0001 0.0004 0.0006 0.0006 0.0006
0.0003 0.0004 0.0005 0.0005
0.0002 0.0002 0.0002
1.5E-05 4E-05
3E-05
LSR 0.05
6E-04 7E-04 7E-04 7E-04 7E-04 7E-04 7E-04
a ab ab ab b b b b
Umur 6 minggu (sesudah uji tantang virus IBD atau ND)
Total
1
2
3
4
5
6
7
8
0.0015 0.0007 0.0013 0.0007 0.001 0.0042
0.0011 0.0009 0.0009 0.0009 0.0009 0.0037
0.0008 0.0019 0.0013 0.0017 0.0015 0.0058
0.0019 0.0025 0.0023 0.0028 0.0024 0.0095
0.0007 0.0012 0.0015
0.0022 0.0009 0.0009 0.0024 0.0016 0.0063
0.00306 0.00383 0.00118 0.00125 0.00233 0.00932
0.0011 0.0009 0.0013
Sumber
db
JK
Perlakuan Galat Total
7 22 29
8.8471E-06 9.2749E-06 1.8122E-05
Per laku an 4 7 6 3 8 5 1 2
Rataan 0.0024 0.0023 0.0016 0.0015 0.0011 0.0011 0.001 0.0009
0.0011 0.0034
KT
F
1.2639E-06 4.2159E-07
0.0011 0.0034
F0.05
2.997873
Perbedaan Antar Perlakuan
SSR 0.05
LSR 0.05
Signifikansi
5E-05 0.0008 0.0009 0.0013 0.0013 0.0013 0.0015
2.93 3.08 3.17 3.24 3.29 3.32 3.35
1E-03 1E-03 0.001 0.001 0.001 0.001 0.001
a a a ab b b b b
0.0007 0.0009 0.0012 0.0012 0.0013 0.0014
0.0001 0.0005 0.0005 0.0005 0.0007
0.0003 0.0003 0.0004 0.0005
2E-06 8E-05 0.0002
7.5E-05 0.0002
0.0001
115
Lampiran 18. Analisis statistika pengaruh perlakuan terhadap berat relatif timus sebelum dan sesudah uji tantang virus IBD atau ND Umur 2 minggu
Total
1
2
3
4
5
6
7
8
0.0067 0.0032 0.006 0.0068 0.0057 0.0227
0.0038 0.0024 0.0035 0.0043 0.0035 0.0141
0.0044 0.0018 0.0011 0.0028 0.0025 0.0101
0.0067 0.001 0.0027 0.003 0.0033 0.0133
0.0004 0.0013 0.0011
0.0003 0.0057 0.0003 0.0037 0.0025 0.01
0.0027 0.00121 0.00528 0.00238 0.00289 0.01158
0.0023 0.0013 0.0039
0.0009 0.0028
Sumber
db
JK
KT
Fhit
F0.05
Perlakuan Galat Total
7 22 29
4.5052E-05 6.7722E-05 1.1277E-04
6.4360E-06 3.0783E-06
2.090761
2.46
0.0025 0.0075
Umur 4 minggu (sesudah uji tantang virus IBD)
Total
1
2
3
4
5
6
7
8
0.0028 0.0023 0.0031 0.0047 0.0032 0.0129
0.0018 0.0056 0.0032 0.0038 0.0036 0.0145
0.0049 0.0023 0.0027 0.0031 0.0033 0.013
0.0078 0.006 0.0045 0.0048 0.0058 0.0231
0.006 0.0048 0.007
0.0057 0.004 0.0039 0.005 0.0047 0.0186
0.00543 0.00236 0.00515 0.00125 0.00355 0.01419
0.0055 0.0021 0.0034
Sumber
db
JK
Perlakuan Galat Total
7 22 29
3.0893E-05 4.4652E-05 7.5545E-05
0.0059 0.0178
KT 4.4134E-06 2.0296E-06
Fhit
F0.05
2.174462
2.46
0.0037 0.011
Umur 5 minggu (sesudah uji tantang virus IBD atau ND)
Total
1
2
3
4
5
6
7
8
0.0045 0.0026 0.0064 0.0063 0.005 0.0199
0.0064 0.0056 0.0009 0.0018 0.0037 0.0146
0.0054 0.0034 0.0012 0.0028 0.0032 0.0129
0.0019 0.0046 0.0045 0.0055 0.0041 0.0165
0.0041 0.0042 0.0049
0.002 0.0038 0.0038 0.0054 0.0037 0.015
0.00186 0.00357 0.00576 0.00323 0.00361 0.01443
0.0046 0.0032 0.0038
0.0044 0.0132
0.0039 0.0116
116
Sumber
db
JK
KT
Perlakuan Galat
7 22
8.0435E-06 6.3088E-05
1.1491E-06 2.8676E-06
Fhit
F0.05
0.400703
2.46
Umur 6 minggu (sesudah uji tantang virus IBD atau ND)
Total
1
2
3
4
5
6
7
8
0.0034 0.0015 0.0031 0.0033 0.0028 0.0112
0.0074 0.0047 0.0038 0.0057 0.0054 0.0216
0.0023 0.0049 0.0022 0.0037 0.0033 0.0131
0.0032 0.0041 0.0026 0.0013 0.0028 0.0112
0.0038 0.0023 0.003
0.0026 0.0037 0.005 0.0053 0.0042 0.0167
0.00344 0.00274 0.00561 0.00453 0.00408 0.01632
0.0045 0.0032 0.0053
Sumber
db
JK
Perlakuan Galat Total
7 22 29
2.2958E-05 3.1241E-05 5.4200E-05
0.003 0.0091
KT 3.2797E-06 1.4201E-06
Fhit 2.309567
F0.05 2.46
0.0044 0.0131
117
Lampiran 19. Tehnik pembuatan preparat histopatologi. Sampel organ diambil dan diawetkan
Sampel dipotong dengan ketebalan ± 3mm Dan difiksasi BNF 10%
Organ kembali dipotong dengan ukuran panjang ± 4mm,lebar 3mm dan tebal 3mm
Pengambilan air dari jaringan (dehidrasi): Organ-organ didehidrasi dengan alkohol konsentrasi bertingkat mulai dar 70%, 80%, 90%, 95% absolut 1 dan absolut 2. Masing-masing konsentrasi membutuhkan waktu ± 2 menit
Tahap penjernihan : Organ dimasukan ke dalam xylol selama 30 menit
Penanaman dalam parafin (embedding dan blok) : Organ-organ tersebut ditanam ke dalam parafin cair dalam lemari pemanas selama 2 jam dan kemudian organ-organ dimasukan ke dalam blok pencetak
Blok parafin dibekukan kemudian disimpan dalam lemari es
Blok dipotong dengan menggunakan mikrotom dengan ketebalan 5 µm (agar hasil potongan tidak mengkerut maka potongan diletakan diatas permukaan air bersuhu 45 oC), hasil potongan kemudian diangkat dengan gelas objek.
Gelas objek tersebut disimpan dan dikeringkan ke dalam inkubator dengan suhu 60 oC selama satu malam
Pewarnaan
118
Lampiran 20. Pewarnaan preparat histologi
Gelas objek yang telah diinkubator dimasukkan ke dalam alkohol konsentrasi bertingkat mulai dari absolut 1, absolut 2, 95%, 90%, 80%, 70% masing-masing selama 2 menit
Lalu bilas dengan air mengalir
Pewarnaan hematoksilin, dilakukan dengan memasukan sediaan ke dalam zat warna hematoksilin selama 2 – 3 menit
Sebelum dimasukkan ke dalam pewarnaan eosin harus dibilas terlebih dahulu dengan air mengalir, demikian pula setelah pewarnaan eosin sediaan kembali dibilas dengan air
Sediaan dimasukkan ke dalam alkohol konsentrasi bertingkat dan alkohol absolut 1 dan 2
Penjernihan : proses ini dilakukan dengan memasukan sediaan ke dalam xylol 1 dan xylol 2 selama beberapa menit
Lalu dikeringkan di udara terbuka agar sediaan terhindar dari pengaruh lingkungan
Kemudian ditutup dengan cover glass yang direkatkan dengan zat perekat entelen
Sediaan kemudian dikeringkan di udara terbuka dan diberi label agar mudah diidentifikasi
Pemeriksaan mikroskopik dilakukan dengan pembesaran 400 x
Perubahan organ pada masing-masing unit perlakuan diamati dengan pengamatan lima lapang pandang
119
Lampiran 21. Skor lesio histopatologi bursa fabricius
1 2 3 4 Rataan 1 R9. Vaksin 2 3 4 Rataan 1 R9. Non Vaksin ND + infeksi ND 2 3 4 Rataan 1 R9. Vaksin + infeksi ND 2 3 4 Rataan 1 R0. Vaksin + infeksi ND 2 3 4 Rataan 1 R9. Non Vaksin IBD + infeksi IBD 2 3 4 Rataan 1 R9. Vaksin + infeksi IBD 2 3 4 Rataan 1 R0. Vaksin + infeksi IBD 2 3 4 Rataan R9. Non Vaksin
2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Umur (minggu) 4 5 1 1 1 1 1 1 1 1 1 1 1 3 1 1 1 0 0 1 1 2 3 1 3 1 2 0 1 1 2.25 0 2 0 3 1 1 1 1 0.5 1.75 1 2 1 3 2 1
6 1 1 1 1 1 2 0 1 1 1 3 2 1 2 2 3 2 1 2 2 2 3 3
1.33 2 2 2 2 2 1 0 1 1 0.75 2 2 1
2.0 3 2 2 3 2.5 1 1 2 2 1.5 1 1 1
2.67 3 2 2 1 2 1 1 1 1 1 2 2 1
1.67
1
1.67
Keterangan : 0 = 0 – 0.25; 1 = 0.26 – 1.25; 2 = 1.26 – 2.25; 3 = 2.26 – 3.00
120
Lampiran 21. Skor lesio histopatologi bursa Fabricius Umur (minggu) 4 5
2 R9. Non Vaksin R9. Vaksin R9. Non Vaksin ND + infeksi ND R9. Vaksin + infeksi ND R0. Vaksin + infeksi ND R9. Non Vaksin IBD + infeksi IBD R9. Vaksin + infeksi IBD R0. Vaksin + infeksi IBD
0 0 0 0 0 0 0 0
1.00 1.00 1.00 0.50 1.33 2.00 0.75 1.67
6
1.00 1.00 2.25 1.75 2.00 2.50 1.50 1.00
1.00 1.00 2.00 2.00 2.67 2.00 1.00 1.67
Uji Kruskal-Walis skor lesio histipatologi bursa Fabricius umur 4 minggu 1
2
3
4
5
13 13 25.5 2.5 13 13 13 13 2.5 13 13 13 13 13 25.5 13 2.5 13 Ri 52 39 54 31 51.5 Ri2 2704 1521 2916 961 2652.25 Ri2/ni 676 507 729 240.25 884.0833 H = [12/(29 x 30)] x Σi Ri2/ni – 3 x 30 H = [12/(29 x 30)] x 7433.229– 3 x 30 H = 12.5273 Hterkoreksi = H/ {(1 – [Σ T/(n-1)n(n+1)]} Hterkoreksi = 12.5273/0.797553 Hterkoreksi = 15.70717; χ20.05; db 7 = 14.1; Hterkoreksi > χ20.05; db 7
6
7
25.5 25.5 25.5 25.5 102 10404 2601
8
13 25.5 2.5 25.5 13 13 13 41.5 64 1722.25 4096 430.5625 1365.333
Uji jarak berganda Duncan skor lesio histipatologi bursa Fabricius umur 4 minggu Perlakuan 6 8 5 2 1 3 7 4
2 1.67 1.33 1 1 1 0.75 0.50
Perbedaan Antar perlakuan
0.33 0.67 1.00 1.00 1.00 1.25 1.50
0.33 0.67 0.67 0.67 0.92 1.17
0.33 0.33 0.33 0.58 0.83
0.00 0.00 0.25 0.50
0.00 0.25 0.50
SSR 0.05
0.25 0.50
0.25
2.93 3.08 3.17 3.24 3.29 3.32 3.35
LSR 0.05
Signifikansi
a 0.75 ab 0.79 abc 0.82 bc 0.83 bc 0.85 bc 0.85 c 0.86 c
121
Uji Kruskal-Walis skor lesio histipatologi bursa Fabricius umur 5 minggu 1 2 3 4 5 9.5 27 27 20 20 9.5 9.5 27 27 27 9.5 1.5 20 9.5 9.5 9.5 1.5 9.5 9.5 38 Ri 39.5 83.5 66 56.5 2 1444 Ri 1560.25 6972.25 4356 3192.25 361 Ri2/ni 390.0625 1743.063 1089 1064.083 2 H = [12/(30 x 31)] x Σi Ri /ni – 3 x 31 H = [12/(30 x 31)] x 7997.208 – 3 x 31 H = 10.18978 Hterkoreksi = H/ {(1 – [Σ T/(n-1)n(n+1)]} Hterkoreksi = 10.18978/0.873637 Hterkoreksi = 11.66363; χ20.05; db 7 = 14.1; Hterkoreksi < χ20.05; db 7
6
7
8
27 20 20 27 94 8836 2209
9.5 9.5 20 20 59 3481 870.25
9.5 9.5 9.5 28.5 812.25 270.75
Uji Kruskal-Walis skor lesio histipatologi bursa Fabricius umur 6 minggu 1
2 3 4 5 8 20 28 28 20 8 1 20 20 28 8 8 8 8 28 8 8 20 20 32 Ri 37 76 76 76 2 1024 Ri 1369 5776 5776 5776 256 Ri2/ni 342.25 1444 1444 1925.333 2 H = [12/(30 x 31)] x Σi Ri /ni – 3 x 31 H = [12/(30 x 31)] x 8311.583 – 3 x 31 H = 14.24624 Hterkoreksi = H/ {1 – [Σ T/(n-1)n(n+1)]} Hterkoreksi = 14.24624/0.865628 Hterkoreksi = 16.45768; χ20.05; db 7 = 14.1; Hterkoreksi > χ20.05; db 7
6
7
8
28 20 20 8 76 5776 1444
8 8 8 8 32 1024 256
20 20 20 60 3600 1200
Uji jarak berganda Duncan skor lesio histipatologi bursa Fabricius umur 6 minggu Perlakuan 5 3 4 6 8 7 1 2
SSR 0.05
Perbedaan Antar Perlakuan 2.67 2 2 2 2 1 1 1
0.67 0.67 0.67 0.67 1.67 1.67 1.67
0 0 0 1 1 1
0 0 1 1 1
0 1 1 1
1 1 1
0 0
0
2.93 3.08 3.17 3.24 3.29 3.32 3.35
LSR 0.05 0.9 1.0 1.0 1.0 1.0 1.0 1.1
Signifikansi a a a a a b b b
122
Lampiran 22. Skor lesio histopatologi limpa
1 2 3 4 Rataan 1 R9. Vaksin 2 3 4 Rataan 1 R9. Non Vaksin ND + infeksi ND 2 3 4 Rataan 1 R9. Vaksin + infeksi ND 2 3 4 Rataan 1 R0. Vaksin + infeksi ND 2 3 4 Rataan 1 R9. Non Vaksin IBD + infeksi IBD 2 3 4 Rataan 1 R9. Vaksin + infeksi IBD 2 3 4 Rataan 1 R0. Vaksin + infeksi IBD 2 3 4 Rataan R9. Non Vaksin
2 0 1 1 1 0.75 0 0 0 1 0.25 0 0 0 0 0 0 1 0 0 0.25 0 0 0 0 0 0 0 1 1 0.5 0 0 0 0 0 0 0 0 0 0
Umur(minggu) 4 5 0 0 1 1 1 0 1 1 0.75 0.50 0 1 0 0 0 0 0 0 0 0.25 0 2 1 2 1 2 0 2 0.5 2 0 2 0 2 0 2 0 2 0 2 0 3 0 3 0 3
6 2 3 1 1 1.75 1 1 1 1 1 2 3 2 2 2.25 1 1 1 1 1 3 3 3
0 1 1 1 0 0.75 0 0 0 0 0 1 0 0
3.0 2 2 2 1 1.75 2 2 1 1 1.50 1 1 2
3.0 2 2 1 1 1.50 2 1 1 1 1.25 2 1 1
0.33
1.33
1.33
Keterangan : 0 = 0 – 0.25; 1 = 0.26 – 1.25; 2 = 1.26 – 2.25; 3 = 2.26 – 3.00
123
Lampiran 22. Skor lesio histopatologi limpa
R9 non vaksin R9 vaksin R9 non vaksin ND+tantang ND R9 vaksin + tantang ND R0 vaksin + tantang ND R9 non vaksin IBD+tantang IBD R9 vaksin+tantang IBD R9 vaksin+tantang IBD
2 0 0 0 0 0 0 0 0
Umur (minggu) 5 0.50 0.83 2.00 0.75 2.10 0.75 0.53 1.03
4 0.50 0.87 0.38 0.63 0.63 0.70 0.68 0.67
Uji kruskal walis skor lesio limpa umur 4 mg 1 2 3 4 9.5 28.5 9.5 9.5 26 1 4 19.5 9.5 15.5 4 38.5 74 24 Ri Ri2 1482.25 5476 576 Ri2/ni 370.5625 1825.333 144 H = [12/(30 x 31)] x Σi Ri2/ni – 3 x 31 H = [12/(30 x 31)] x7334.604 – 3 x 31 H = 8.166954 χ20.05; db 7 = 14.1; H > χ20.05; db 7
5
22.5 22.5 9.5 4 58.5 3422.25 855.5625
Uji kruskal walis skor lesio limpa umur 5 mg 1 2 3 3.50 12.50 24.00 6.00 25.00 28.00 9.50 6.00 21.00 12.50 16.50 30.00 Ri 31.5 60 103 Ri2 992.25 3600 10609 2 Ri /ni 248.0625 900 2652.25 H = [12/(30 x 31)] x Σi Ri2/ni – 3 x 31 H = [12/(30 x 31)] x 8541.354 – 3 x 31 H = 17.21102 χ20.05; db 7 = 14.1; H> χ20.05; db 7
6 0.45 0.38 1.85 0.85 1.97 1.60 0.80 1.73
9.5 22.5 15.5 47.5 2256.25 752.0833
4
26.00 1.50 9.50 1.50 38.5 1482.25 370.5625
6
7
26 15.5 15.5 19.5 76.5 5852.25 1463.063
4 15.5 26 22.5 68 4624 1156
5 28.00 21.00 28.00 77 5929 1976.333
8 4 15.5 28.5 48 2304 768
6
7
12.50 16.50 12.50 16.50 58 3364 841
19.00 6.00 3.50 8.00 36.5 1332.25 333.0625
8 21.00 23.00 16.50 60.5 3660.25 1220.083
124
Uji jarak berganda Duncan skor lesio limpa umur 5 minggu Perlakuan 5 3 8 2 4 6 7 1
Perbedaan Antar Perlakuan 2.10 2.00 1.03 0.83 0.75 0.75 0.53 0.50
0.10 1.07 1.28 1.35 1.35 1.58 1.60
0.97 1.18 1.25 1.25 1.48 1.50
0.21 0.28 0.28 0.51 0.53
0.08 0.08 0.30 0.33
2.93 3.08 3.17 3.24 0.00 3.29 0.23 0.23 3.32 0.25 0.25 0.03 3.35
Uji kruskal walis skor lesio limpa umur 6 mg 1 2 3 4 1.50 11.50 5.50 15.00 3.50 29.00 11.50 7.50 25.00 1.50 5.50 26.00 Ri 29.5 28 85.5 Ri2 870.25 784 7310.25 Ri2/ni 217.5625 196 1827.563 H = [12/(30 x 31)] x Σi Ri2/ni – 3 x 31 H = [12/(30 x 31)] x 8371.917 – 3 x 31 H = 15.02473 χ20.05; db 7 = 14.1; H > χ20.05; db 7
SSR LSR Signifi0.05 0.05 kansi
9.00 18.00 7.50 19.00 53.5 2862.25 715.5625
5
a 0.8 ab 0.9 bc 0.9 c 0.9 c 0.9 c 1 c 1 c
6
30.00 23.00 17.00 70 4900 1633.333
11.50 27.50 27.50 15.00 81.5 6642.25 1660.563
Uji jarak berganda Duncan skor lesio limpa umur 6 minggu Perlalakuan 5 3 8 6 4 7 1 2
Perbedaan Antar Perlakuan 1.97 1.85 1.73 1.60 0.85 0.80 0.45 0.38
0.12 0.24 0.37 1.12 1.17 1.52 1.60
0.12 0.25 1.00 1.05 1.40 1.48
0.13 0.88 0.93 1.28 1.36
0.75 0.80 1.15 1.23
SSR LSR Signifi0.05 0.05 kansi
2.93 3.08 3.17 3.24 0.05 3.29 0.40 0.35 3.32 0.48 0.43 0.08 3.35
a 0.99 ab 1.04 ab 1.07 abc 1.09 bc 1.11 bc 1.12 c 1.13 c
7 3.50 20.00 15.00 11.50 50 2500 625
8 24.00 21.00 22.00 67 4489 1496.333
125
Lampiran 23. Skor lesio histopatologi timus
R9. Non Vaksin
R9. Vaksin
R9. Non Vaksin ND + infeksi ND
R9. Vaksin + infeksi ND
R0. Vaksin + infeksi ND
R9. Non Vaksin IBD + infeksi IBD
R9. Vaksin + infeksi IBD
R0. Vaksin + infeksi IBD
2 0 1 1 0 0.50 0 0 1
Umur 4 1 0 2 2 1.25 1 1 0
0.33 0
2 3 4 Rataan 1 2 3 4 Rataan 1 2 3 4 Rataan 1 2 3 4 Rataan 1 2 3 4 Rataan 1 2 3 4 Rataan
1 2 3 4 Rataan 1 2 3 4 Rataan 1
0.67 2
(minggu) 5 1 1 1 0 0.75 1 0 0 0 0.25 1
6 1 1 3 2 1.75 1 1 1 1 1.00 3
1 0 1 0.50 1 0 0 0 0.25 0 0 1
1 0 2 1.25 1 0 0 0 0.25 0 0 1
1 1 0 0.75 1 1 2 1 1.25 1 1 1
3 3 3 3.00 1 2 2 2 1.75 3 2 1
0.33 0
0.33 1
1.00 2
2.00 2
0 1 1 0.50 1 0 0 0.33 0 0 1
1 0 0 0.50 0 1 0 0 0.25 1 2 1
2 1 1 1.50 1 1 2 2 1.50 2 2 2
2 3 2 2.25 2 1 2 1 1.50 3 2 2
0.33
1.33
2.00
2.33
126
Lampiran 23. Skor lesio histopatologi timus Umur (minggu) 4 5
2 R9. Non Vaksin R9. Vaksin R9. Non Vaksin ND + infeksi ND R9. Vaksin + infeksi ND R0. Vaksin + infeksi ND R9. Non Vaksin IBD + infeksi IBD R9. Vaksin + infeksi IBD R0. Vaksin + infeksi IBD
0.50 0.33 0.50 0.25 0.33 0.5 0.33 0.33
1.25 0.67 1.25 0.25 0.33 0.50 0.25 1.33
6
0.75 0.25 0.75 1.25 1.00 1.50 1.50 2.00
1.75 1.00 3.00 1.75 2.00 2.25 1.50 2.33
Uji Kruskal-Walis skor lesio histipatologi timus umur 2 minggu 1
2
3
4
5
9 9 9 23 9 23 9 23 9 9 23 23 9 9 23 9 23 9 Ri 64 41 64 50 41 Ri2 4096 1681 4096 2500 1681 Ri2/ni 1024 560.3333 1024 625 560.3333 H = [12/(28 x 29)] x Σi Ri2/ni – 3 x 29 H = [12/(28 x 29)]x 5938.333 - 3 x 29 H = 0.758621 Hterkoreksi = H/ {1 – [Σ T/(n-1)n(n+1)]} Hterkoreksi = 0.758621/0.716475 Hterkoreksi =1.058824; χ20.05; db 7 = 14.1; Hterkoreksi < χ20.05; db 7
6
7
8
9 9 23 23 64 4096 1024
23 9 9
9 9 23
41 1681 560.333
41 1681 560.333
Uji Kruskal-Walis skor lesio histipatologi timus umur 4 minggu 1
2
3
4
5
19 19 27 19 7 7 19 19 7 7 27 7 7 7 19 27 27 7 Ri 80 45 80 40 33 2 Ri 6400 2025 6400 1600 1089 Ri2/ni 1600 675 1600 400 363 H = [12/(29 x 30)] x Σi Ri2/ni – 3 x 30 H = [12/(29 x 30)] x7122.333 – 3 x 30 H = 8.23908 Hterkoreksi = H/ {1 – [Σ T/(n-1)n(n+1)]} Hterkoreksi = 8.23908/0.851232 Hterkoreksi =9.679012; χ20.05; db 7 = 14.1; Hterkoreksi < χ20.05; db 7
6
7
8
19 19 7 7
7 19 7 7
19 27 19
52 2704 676
40 1600 400
65 4225 1408.333
127
Uji Kruskal-Walis skor lesio histipatologi timus umur 5 minggu 1
2
3
4
5
14 14 14 14 14 14 3 14 14 14 14 3 14 26.5 14 3 3 3 14 45 23 45 68.5 42 Ri Ri2 2025 529 2025 4692.25 1764 Ri2/ni 506.25 132.25 506.25 1173.06 588 H = [12/(30 x 31)] x Σi Ri2/ni – 3 x 31 H = [12/(30 x 31)] x 8293.063 – 3 x 31 H = 14.00726 Hterkoreksi = H/ {1 – [Σ T/(n-1)n(n+1)]} Hterkoreksi = 14.00726/0.795328 Hterkoreksi =17.61192; χ20.05; db 7 = 14.1; Hterkoreksi > χ20.05; db 7
6
7
8
26.5 26.5 14 14 81 6561 1640.25
14 14 26.5 26.5 81 6561 1640.25
26.5 26.5 26.5 79.5 6320.25 2106.75
Uji jarak berganda Duncan skor lesio histipatologi timus umur 5 minggu Perlakuan 8 6 7 4 5 1 3 2
Rataan
Perbedaan Antar Perlakuan
2 1.5 1.5 1.25 1 0.75 0.75 0.25
0.5 0.5 0.75 1 1.25 1.25 1.75
0 0.25 0.5 0.75 0.75 1.25
0.25 0.5 0.75 0.75 1.25
0.25 0.5 0.5 1
0.25 0.25 0.75
0 0.5
0.5
SSR 0.05
LSR 0.05
Signifikansi
2.93 3.08 3.17 3.24 3.29 3.32 3.35
0.70 0.73 0.76 0.77 0.78 0.79 0.80
a ab ab ab bc bc bc c
Uji Kruskal-Walis skor lesio histipatologi timus umur 6 minggu 1
2
3
4
5
5.5 5.5 27 17 5.5 5.5 5.5 27 17 17 27 5.5 27 17 5.5 17 5.5 27 17 Ri 55 22 108 68 28 Ri2 3025 484 11664 4624 784 Ri2/ni 756.25 121 2916 1156 261.333 2 H = [12/(30 x 31)] x Σi Ri /ni – 3 x 31 H = [12/(30 x 31)] x 8478.167 – 3 x 31 H = 16.3957 Hterkoreksi = H/ {1 – [Σ T/(n-1)n(n+1)]} Hterkoreksi = 16.3957/0.869855 Hterkoreksi =18.84876; χ20.05; db 7 = 14.1; Hterkoreksi > χ20.05; db 7
6 17 17 27 17 78 6084 1521
7
8
17 5.5 17 5.5 45 2025 506.25
27 17 17 61 3721 1240.33
128
Uji jarak berganda Duncan skor lesio histipatologi timus umur 6 minggu Perla- Ratakuan an 3 8 6 5 4 1 7 2
3.00 2.33 2.25 2.00 1.75 1.75 1.50 1.00
Perbedaan Antar Perlakuan
0.67 0.75 1.00 1.25 1.25 1.50 2.00
0.08 0.33 0.58 0.58 0.83 1.33
0.25 0.50 0.50 0.75 1.25
0.25 0.25 0.50 1.00
0.00 0.25 0.75
SSR 0.05
0.25 0.75
2.93 3.08 3.17 3.24 3.29 3.32 0.50 3.35
LSR 0.05
Signifi-
a 0.8788 ab 0.9238 ab 0.9508 b 0.9718 bc 0.9868 bc 0.9958 bc 1.0048 c
