Lampiran 1. Komposisi media RPMI-1640 Komponen Garam anorganik Ca(NO3)2.4H2O KCl MgSO4.7H2O NaCl NaHCO3 Na2HPO4 anhydrous Komponen lain D-Glukosa fenol merah glutathione (tereduksi) HEPES (d) Asam Amino L-arginin HCl L-asparagin L-asam aspartat L-sistin L-asam glutamat L-glutamin Glisin L-histidin L-hidroksiprolin L-isoleusin L-leusin L-lisin HCl L-metionin L-fenilalanin L-prolin L-serin L-threonin L-triptofan L-tirosin L-valin Vitamin D-biotin B-Ca pantotenat kolin klorida asam folat i-inositol
Konsentrasi (mg/l) 100 400 100 6000 2000 800 2000 5,3 1 5958 200 56,82 20 50 20 300 10 15 20 50 50 40 15 15 20 30 20 5 20 20 0,2 0,25 3 1 35
113
Lanjutan tabel 1 Komponen nikotinamida PABA Piridoksal HCl Riboflavin Tiamin HCl Vitamin B12
Konsentrasi (mg/l) 1 1 1 0,2 1 0,005
114
Lampiran 2. Komposisi balanced salt solution PBS (Harrison and Rae, 1997) Komponen CaCl2.H2O KCl KH2PO4 MgCl2.6H2O NaCl Na2HPO4
Konsentrasi (mg/l) 132,5 200 200 100 8000 1150
115
Lampiran 3. Proses Produksi Pangan Iradiasi yang shelf-stable (IAEA, 1995) Langkah kunci dalam produksi pangan iradiasi adalah sebagai berikut: 1. Masaklah terlebih dahulu daging mentah, unggas, dan seafood sampai keadaan matang (medium done) dengan syarat suhu internalnya kira-kira 75°C, untuk menginaktivasi enzim pengurai jaringan, kecuali bahan yang dimasak telah diproses secara termal sebelumnya (misalnya diasapkan (hot-smoked), dikuring, atau dibuat pastrami) 2. Secara hermetis kemaslah secara vakum dalam kemasan individu yang berupa kantong multilapis alumunium foil, seperti yang dikembangkan oleh program US Army dan disetujui oleh FDA atau yang setara. 3. Bekukan produk dalam kemasan secara kriogenik atau mekanis hingga mencapai -40°C dan iradiasi saat masih dalam keadaan beku untuk mempertahankan kualitas gizi. Menumpuk kemasan dengan dry ice merupakan cara yang efektif dan tidak mahal untuk mempertahankan keadaan beku selama proses iradiasi yang panjang. 4. Untuk iradiasi gamma atau sinar X, kemas kantong-kantong pengemas individual beku dalam karton pengemas dan simpan dalam keadaan beku atau dengan dry ice sebelum diiradiasi. Jika waktu iradiasi lebih dari satu jam, dry ice harus ditaruh di sekitar karton pengemas untuk mempertahankan keadaan beku (hard frozen) selama proses iradiasi. Bahkan pada energy maksimum 10 MeV iradiasi electron /electron beam (yang memiliki daya penetrasi rendah) harus diterapkan pada tiap kantong produk yang dibekukan, misalnya: kantong yang diletakkan dalam posisi tidur pada ban berjalan yang membawa produk-produk tersebut saat diiradiasi dengan electron beam. Kantong yang berukuran lebih tebal daripada beberapa sentimeter harus diiradiasi pada tiap sisi (two-pass, 50% dari total minimum dosis pada tiap kali) untuk mencapai keseragaman. Laju dosis iradiasi dengan electron beam normalnya merupakan dosis yang sangat tinggi sehingga tidak membutuhkan penanganan aktif untuk mempertahankan keadaan beku selama iradiasi (waktu paparan normalnya
116
terlalu sebentar untuk kemungkinan terjadinya kenaikan suhu yang signifikan). 5. Lakukan proses iradiasi dengan dosis minimum yang telah ditentukan (2545 kGy tergantung produknya) untuk memberikan tingkat 10 -12 jaminan sterilitas antibotulinum dengan menggunakan dosimetri yang sesuai. Dosis minimum yang dibutuhkan untuk berbagai produk telah ditentukan oleh studi produk yang diinokulasi dengan C. botulinum yang dilakukan oleh program US Army. Pada level dosis minimum ini, produk steril secara mikrobiologi dan dapat disimpan selama bertahun-tahun dalam kondisi suhu ruang karena enzim endogen yang akan mendegradasi produk, meskipun tidak ada mikroorganisme yang hidup, telah diinaktivasi dengan panas sebelum dikemas dan diiradiasi. 6. Segera setelah iradiasi, biarkan suhu produk naik secara perlahan hingga mencapai suhu ruang. Kemas secara individu kantong-kantong produk dalam karton pengemas seperti saat sebelum diiradiasi.
117
Lampiran 4. Tabel data hasil pembacaan absorbansi uji proliferasi limfosit
Sampel
11 Nov 06
DIPA 14 Jun 07
No label 14 Jun 07
Kontrol Rendang
K+LPS
K+PWM
K-
1x
2x
4x
2,170 1,592 1,666 2,385 2,228 1,939 1,882 2,777 2,161 1,478 1,68 1,633 1,155 1,504 1,377 1,992 1,683 0,741 1,094 1,321 1,428
2,338 1,099 2,052 1,44 1,431 1,745 0,783 1,478 1,612 1,413 1,229 1,196
2,436 1,693 1,553 1,208 1,528 1,788 1,324 1,303 1,887 1,345 1,536 1,338
118
Lampiran 5. Contoh cara perhitungan untuk mendapatkan Indeks Stimulasi (I.S.) proliferasi limfosit
*Sampel 11 November 06, pengenceran 1x Diketahui :
Absorbansi sampel = 2,170 Absorbansi kontrol negatif = 1,904
maka I.S. =
Abs ekstrak sampel Abs kontrol negatif
I.S. =
2,170 1,094
I.S. = 1,984
119
Lampiran 6. Tabel data hasil pembacaan absorbansi uji hemolisis eritrosit pengenceran 1x
0 0,120 0,117 0,121 0,162 0,119 0,114 0,137 0,143
Waktu pengamatan (jam) 1 2 3 0,122 0,128 0,128 0,119 0,119 0,118 0,130 0,125 0,143 0,127 0,133 0,122 0,126 0,122 0,122 0,118 0,122 0,116 0,137 0,138 0,142 0,159 0,164 0,172
4 0,132 0,124 0,130 0,129 0,130 0,124 0,137 0,171
5 0,137 0,121 0,132 0,131 0,132 0,123 0,141 0,179
0,126 0,166 0,134 0,106 0,105 0,108 0,095 0,105 0,107
0,126 0,164 0,131 0,103 0,104 0,119 0,101 0,122 0,105
0,117 0,182 0,123 0,116 0,108 0,147 0,111 0,151 0,117
0,129 0,173 0,133 0,123 0,115 0,160 0,115 0,162 0,121
Sampel
11 Nov 2006 1x
DIPA 14 Jun 2007 1x
No Label 14 Jun 2007 1x
Kontrol Rendang 1x
K+ 1x
K- 1x
0,128 0,168 0,131 0,117 0,113 0,134 0,107 0,132 0,113
0,180 0,170 0,138 0,114 0,108 0,134 0,106 0,138 0,110
120
Lampiran 7. Tabel data hasil pembacaan absorbansi uji hemolisis eritrosit pengenceran 2x
Sampel
11 Nov 2006 2x
DIPA 14 Jun 2007 2x
No Label 14 Jun 2007 2x
Kontrol Rendang 2x
0 0,127 0,110 0,108 0,114 0,126 0,114 0,140 0,120 0,119 0,122 0,119 0,134
Waktu pengamatan (jam) 1 2 3 0,139 0,145 0,151 0,112 0,113 0,112 0,108 0,108 0,109 0,112 0,135 0,113 0,131 0,109 0,130 0,120 0,122 0,123 0,137 0,137 0,132 0,115 0,118 0,112 0,113 0,116 0,108 0,131 0,141 0,130 0,108 0,117 0,111 0,142 0,146 0,146
4 0,150 0,115 0,112 0,114 0,132 0,132 0,142 0,117 0,112 0,158 0,112 0,149
5 0,155 0,116 0,112 0,114 0,137 0,133 0,140 0,120 0,112 0,144 0,114 0,153
121
Lampiran 8. Tabel data hasil pembacaan absorbansi uji hemolisis eritrosit pengenceran 4x
Sampel
11 Nov 2006 4x
DIPA 14 Jun 2007 4x
No Label 14 Jun 2007 4x
Kontrol Rendang 4x
0 0,122 0,109 0,110 0,115 0,111 0,099 0,109 0,106 0,108 0,109 0,104 0,109
Waktu pengamatan (jam) 1 2 3 0,136 0,159 0,200 0,107 0,106 0,111 0,101 0,102 0,103 0,107 0,113 0,112 0,106 0,111 0,115 0,100 0,103 0,104 0,119 0,116 0,113 0,118 0,112 0,111 0,109 0,113 0,106 0,101 0,106 0,103 0,102 0,106 0,105 0,112 0,116 0,113
4 0,200 0,110 0,106 0,111 0,106 0,104 0,119 0,118 0,113 0,106 0,106 0,116
5 0,226 0,108 0,106 0,115 0,106 0,104 0,120 0,124 0,115 0,100 0,103 0,113
122
Lampiran 9. Contoh cara perhitungan % hemolisis *sampel 11 Nov 06, pengenceran 1x jam ke-5
Diketahui:
Absorbansi sampel
= 0,137
Absorbansi Kontrol (-) = 0,115
% Hemolisis =
=
= 119,130%
123
Lampiran 10. Tabel data hasil pembacaan absorbansi pengukuran kapasitas antioksidan (metode DPPH) Sampel 11 Nov 06 DIPA 14 Jun 07 No label 14 Jun 07 Kontrol Rendang
1x 1,190 1,193 1,050 1,051 1,060 1,060 0,995 0,996
Absorbansi 2x
4x
1,035
1,020
1,030
1,024
1,030
1,020
1,033 1,055
1,022 1,045
1,057
1,040
1,025
1,045
1,025
1,044
124
Lampiran 11. Contoh perhitungan untuk mendapatkan % kapasitas antioksidan tersisa *sampel 11 Nov 06 pengenceran 1x ulangan 1
Diketahui: Abs sampel: 1,190 Abs kontrol (-): 1,350 maka % Kapasitas Antioksidan tersisa
=
= = 11,85% Kapasitas ini setara dengan kapasitas antioksidan vitamin C (x) sebesar: y = 1,3227 – 0,0011x 1,190 = 1,3227 – 0,0011x x = 120,64 ppm
125
Lampiran 12. Tabel data hasil pembacaan absorbansi pengukuran kadar Malonaldehida Sampel 11 Nov 06 DIPA 14 Jun 07 No label 14 Jun 07 Kontrol Rendang
1x 0,458 0,432 0,464 0,370 0,462 0,538 0,542 0,562
Absorbansi 2x
4x
0,250
0,170
0,238
0,172
0,283
0,166
0,207
0,110
0,270
0,180
0,369
0,213
0,340
0,230
0,310
0,225
126
Lampiran 13. Contoh perhitungan untuk mendapatkan kadar malonaldehida *sampel 11 Nov 06 pengenceran 1x ulangan 1
Diketahui :
Abs sampel : 0,458 (y)
Dengan memasukkan nilai y = 0,458 ke persamaan y= 0,0025x + 0,1172 maka y
= 0,0025x + 0,1172
0,458 = 0,0025x + 0,1172 0,3408 = 0,0025x x
= 136,32 pmol/ml
127
Lampiran 14. Hasil analisis statistik uji proliferasi limfosit pada selang kepercayaan 95%
Univariate Analysis of Variance Between-Subjects Factors N Sampel
Ulangan
11 Nov 06 DIPA14Jun Kontrol_R
3 3
Ktrl Std
3
LPS
3
NL14Jun
3 3 7 7 7
3
PWM 1 2 3
Tests of Between-Subjects Effects Dependent Variable: Absorbansi Source Model
Type III Sum of Squares 44,445(a)
Sampel Ulangan Error Total
Df 9
Mean Square 4,938
2,754 6 ,660 2 1,350 12 45,795 21 a R Squared = ,971 (Adjusted R Squared = ,948)
,459 ,330 ,113
F 43,894
Sig. ,000
4,080 2,932
,018 ,092
Post Hoc Tests Sampel
128
Multi ple Comparisons Dependent Variable: Absorbansi Tukey HSD
(I) Sampel 11 Nov 06
DIPA14Jun
Kontrol_R
Ktrl Std
LPS
NL14Jun
PWM
(J) Sampel DIPA14Jun Kontrol_R Ktrl Std LPS NL14Jun PWM 11 Nov 06 Kontrol_R Ktrl Std LPS NL14Jun PWM 11 Nov 06 DIPA14Jun Ktrl Std LPS NL14Jun PWM 11 Nov 06 DIPA14Jun Kontrol_R LPS NL14Jun PWM 11 Nov 06 DIPA14Jun Kontrol_R Ktrl Std NL14Jun PWM 11 Nov 06 DIPA14Jun Kontrol_R Ktrl Std LPS PWM 11 Nov 06 DIPA14Jun Kontrol_R Ktrl Std LPS NL14Jun
Mean Dif f erence (I-J) Std. Error -,28967 ,273871 ,19633 ,273871 ,45200 ,273871 ,39900 ,273871 -,62567 ,273871 ,24733 ,273871 ,28967 ,273871 ,48600 ,273871 ,74167 ,273871 ,68867 ,273871 -,33600 ,273871 ,53700 ,273871 -,19633 ,273871 -,48600 ,273871 ,25567 ,273871 ,20267 ,273871 -,82200 ,273871 ,05100 ,273871 -,45200 ,273871 -,74167 ,273871 -,25567 ,273871 -,05300 ,273871 -1,07767* ,273871 -,20467 ,273871 -,39900 ,273871 -,68867 ,273871 -,20267 ,273871 ,05300 ,273871 -1,02467* ,273871 -,15167 ,273871 ,62567 ,273871 ,33600 ,273871 ,82200 ,273871 1,07767* ,273871 1,02467* ,273871 ,87300 ,273871 -,24733 ,273871 -,53700 ,273871 -,05100 ,273871 ,20467 ,273871 ,15167 ,273871 -,87300 ,273871
Sig. ,929 ,989 ,657 ,763 ,324 ,965 ,929 ,586 ,177 ,235 ,871 ,482 ,989 ,586 ,959 ,987 ,112 1,000 ,657 ,177 ,959 1,000 ,024 ,986 ,763 ,235 ,987 1,000 ,033 ,997 ,324 ,871 ,112 ,024 ,033 ,083 ,965 ,482 1,000 ,986 ,997 ,083
95% Conf idence Interv al Lower Bound Upper Bound -1,24818 ,66885 -,76218 1,15485 -,50652 1,41052 -,55952 1,35752 -1,58418 ,33285 -,71118 1,20585 -,66885 1,24818 -,47252 1,44452 -,21685 1,70018 -,26985 1,64718 -1,29452 ,62252 -,42152 1,49552 -1,15485 ,76218 -1,44452 ,47252 -,70285 1,21418 -,75585 1,16118 -1,78052 ,13652 -,90752 1,00952 -1,41052 ,50652 -1,70018 ,21685 -1,21418 ,70285 -1,01152 ,90552 -2,03618 -,11915 -1,16318 ,75385 -1,35752 ,55952 -1,64718 ,26985 -1,16118 ,75585 -,90552 1,01152 -1,98318 -,06615 -1,11018 ,80685 -,33285 1,58418 -,62252 1,29452 -,13652 1,78052 ,11915 2,03618 ,06615 1,98318 -,08552 1,83152 -1,20585 ,71118 -1,49552 ,42152 -1,00952 ,90752 -,75385 1,16318 -,80685 1,11018 -1,83152 ,08552
Based on observ ed means. *. The mean dif f erence is signif icant at the ,05 lev el.
129
Homogeneous Subsets Absorbansi Tukey HSD Subset Sampel Ktrl Std
3
1 1,00000
3 3
1,05300 1,20467
1,20467
Kontrol_R 11 Nov 06 DIPA14Jun
3 3
1,25567 1,45200
1,25567 1,45200
3
1,74167
1,74167
NL14Jun
3 ,177
2,07767 ,083
LPS PWM
Sig.
N
2
Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = ,113. a Uses Harmonic Mean Sample Size = 3,000. b Alpha = ,05.
130
Lampiran 15. Hasil analisis statistik uji proliferasi limfosit semua sampel pada selang kepercayaan 99%
Univariate Analysis of Variance Between-Subjects Factors N Sampel
11 Nov 06 DIPA14Jun
3 3 3
Kontrol_R Ktrl Std
Ulangan
LPS NL14Jun
3 3 3
PWM 1 2 3
3 7 7 7 Tests of Between-Subjects Effects
Dependent Variable: Absorbansi Source Model Sampel Ulangan Error
Type III Sum of Squares 44,445(a) 2,754 ,660 1,350
df
Mean Square 9 6 2 12
4,938 ,459 ,330 ,113
F 43,894 4,080 2,932
Sig. ,000 ,018 ,092
Total
45,795 21 a R Squared = ,971 (Adjusted R Squared = ,948)
131
Lampiran 16. Hasil analisis statistik uji proliferasi limfosit sampel 11 Nov 06 pada berbagai pengenceran pada selang kepercayaan 95%
Univariate Analysis of Variance Between-Subjects Factors N Sampel
11Nov06_1
3 3 3
Ulangan
11Nov06_2 11Nov06_4 1
3 3 3
2 3
Tests of Between-Subjects Effects Dependent Variable: IndeksStimulasi Source Model Sampel Ulangan Error
Type III Sum of Squares 21,659(a) ,011 1,819
df 5
Mean Square 4,332
F 85,677
Sig. ,000
2 2
,005 ,909
,107 17,986
,901 ,010
,202 4 21,861 9 a R Squared = ,991 (Adjusted R Squared = ,979)
,051
Total
132
Lampiran 17. Hasil analisis statistik uji proliferasi limfosit sampel DIPA 14 Juni 07 pada berbagai pengenceran pada selang kepercayaan 95%
Univariate Analysis of Variance Between-Subjects Factors N Sampel
Ulangan
DIPA_1 DIPA_2
3
DIPA_4
3 3
3
1 2
3 3
3
Tests of Between-Subjects Effects Dependent Variable: IndeksStimulasi Source Model Sampel Ulangan Error Total
Type III Sum of Squares 19,208(a) ,447 ,038 ,473 19,681
df 5 2 2 4 9
Mean Square 3,842 ,224 ,019 ,118
F 32,477 1,890 ,159
Sig. ,002 ,264 ,858
a R Squared = ,976 (Adjusted R Squared = ,946)
133
Lampiran 18. Hasil analisis statistik uji proliferasi limfosit sampel No Label 14 Juni 07 pada berbagai pengenceran pada selang kepercayaan 95%
Univariate Analysis of Variance Between-Subjects Factors N Sampel
Ulangan
NL_1 NL_2
3
NL_3
3 3
3
1 2
3 3
3
Tests of Between-Subjects Effects Dependent Variable: IndeksStimulasi Source Model Sampel Ulangan Error
Type III Sum of Squares 20,185(a) 2,041 ,183
df 5
Mean Square 4,037
F 47,989
Sig. ,001
2 2
1,020 ,092
12,131 1,089
,020 ,419
,336 4 20,521 9 a R Squared = ,984 (Adjusted R Squared = ,963)
,084
Total
Post Hoc Tests Sampel Multi ple Comparisons Dependent Variable: IndeksStimulasi Tukey HSD
(I) Sampel NL_1 NL_2 NL_3
(J) Sampel NL_2 NL_3 NL_1 NL_3 NL_1 NL_2
Mean Dif f erence (I-J) St d. Error 1,08967* ,236815 ,90533* ,236815 -1,08967* ,236815 -,18433 ,236815 -,90533* ,236815 ,18433 ,236815
Sig. ,022 ,040 ,022 ,735 ,040 ,735
95% Conf idence Interv al Lower Bound Upper Bound ,24566 1,93367 ,06133 1,74934 -1,93367 -,24566 -1,02834 ,65967 -1,74934 -,06133 -,65967 1,02834
Based on observ ed means. *. The mean dif f erence is signif icant at t he ,05 lev el.
134
Homogeneous Subsets IndeksStimulasi Tukey HSD Subset Sampel NL_2 NL_3 NL_1 Sig.
N 3 3 3
1 ,98800
2
1,17233 ,735
2,07767 1,000
Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) = ,084. a Uses Harmonic Mean Sample Size = 3,000. b Alpha = ,05.
135
Lampiran 19. Hasil analisis statistik uji proliferasi limfosit sampel No Label 14 Juni 07 pada berbagai pengenceran pada selang kepercayaan 99%
Univariate Analysis of Variance Between-Subjects Factors N Sampel
Ulangan
NL_1 NL_2
3 3 3 3 3
NL_3 1 2 3
3 Tests of Between-Subjects Effects
Dependent Variable: IndeksStimulasi Source Model Sampel Ulangan
Type III Sum of Squares 20,185(a) 2,041
df 5 2
Mean Square 4,037 1,020
,183 2 ,336 4 20,521 9 a R Squared = ,984 (Adjusted R Squared = ,963) Error Total
,092 ,084
F 47,989 12,131
Sig. ,001 ,020
1,089
,419
136
Lampiran 20. Hasil analisis statistik uji proliferasi limfosit sampel Kontrol Rendang pada berbagai pengenceran pada selang kepercayaan 95%
Univariate Analysis of Variance Between-Subjects Factors N Sampel
Ulangan
K_1 K_2 K_3 1 2 3
3 3 3 3 3 3
Tests of Between-Subjects Effects Dependent Variable: IndeksStimulasi Source Model Sampel Ulangan Error Total
Type III Sum of Squares 11,697(a)
df 5
Mean Square 2,339
,085 2 ,152 2 ,032 4 11,729 9 a R Squared = ,997 (Adjusted R Squared = ,994)
,042 ,076 ,008
F 289,202
Sig. ,000
5,247 9,369
,076 ,031
137
Lampiran 21. Hasil analisis statistik uji hemolisis eritrosit sampel 11 Nov 06 pada jam ke-5 pada selang kepercayaan 95%
Univariate Analysis of Variance Between-Subjects Factors N Sampel
Ulangan
1,00
3
2,00 3,00
3
1,00
3 3 3
2,00 3,00
3
Tests of Between-Subjects Effects Dependent Variable: Absorbansi Source Model Sampel Ulangan Error Total
Ty pe II I Sum of Squares ,171a ,001 ,006 ,004 ,175
df 5 2 2 4 9
Mean Square ,034 ,000 ,003 ,001
F 32,179 ,304 3,048
Sig. ,003 ,754 ,157
a. R Squared = , 976 (Adjusted R Squared = ,945)
138
Lampiran 22. Hasil analisis statistik uji hemolisis eritrosit sampel DIPA 14 Juni 2007 pada jam ke-5 pada selang kepercayaan 95%
Univariate Analysis of Variance
Between-Subjects Factors N Sampel
Ulangan
1,00 2,00 3,00 1,00 2,00 3,00
3 3 3 3 3 3
Tests of Between-Subjects Effects Dependent Variable: Absorbansi Source Model Sampel Ulangan Error Total
Ty pe II I Sum of Squares ,134a ,001 5,00E-005 ,000 ,134
df 5 2 2 4 9
Mean Square ,027 ,000 2,50E-005 9,23E-005
F 290,417 4,336 ,271
Sig. ,000 ,100 ,776
a. R Squared = , 997 (Adjusted R Squared = ,994)
139
Lampiran 23. Hasil analisis statistik uji hemolisis eritrosit sampel No Label 14 Juni 2007 pada jam ke-5 pada selang kepercayaan 95%
Univariate Analysis of Variance Between-Subjects Factors N Sampel
Ulangan
1,00 2,00 3,00 4,00 1,00 2,00 3,00
3 3 3 3 4 4 4
Tests of Between-Subjects Effects Dependent Variable: Absorbansi Source Model Sampel Ulangan Error Total
Ty pe II I Sum of Squares ,198a ,002 ,001 ,001 ,199
df 6 3 2 6 12
Mean Square ,033 ,001 ,000 ,000
F 186,486 3,823 1,538
Sig. ,000 ,076 ,289
a. R Squared = , 995 (Adjusted R Squared = ,989)
140
Lampiran 24. Hasil analisis statistik uji hemolisis eritrosit sampel kontrol pada jam ke-5 pada selang kepercayaan 95%
Univariate Analysis of Variance
Between-Subjects Factors N Sampel
Ulangan
1,00 2,00 3,00 1,00 2,00 3,00
3 3 3 3 3 3
Tests of Between-Subjects Effects Dependent Variable: Absorbansi Source Model Sampel Ulangan Error Total
Ty pe II I Sum of Squares ,162a ,003 ,000 ,001 ,163
df 5 2 2 4 9
Mean Square ,032 ,002 ,000 ,000
F 134,475 6,895 ,769
Sig. ,000 ,051 ,522
a. R Squared = , 994 (Adjusted R Squared = ,987)
141
Lampiran 25. Hasil analisis statistik persen hemolisis eritrosit semua sampel pada jam ke-5 pada selang kepercayaan 95%
Univariate Analysis of Variance Between-Subjects Factors N Sampel
Ulangan
1,00 2,00 3,00 4,00 5,00 1,00 2,00 3,00
3 3 2 3 3 5 5 4
Tests of Between-Subjects Effects Dependent Variable: PersenHemo Source Model Sampel Ulangan Error Total
Ty pe I II Sum of Squares 157585,422a 726,438 2295,430 1309,665 158895,088
df 7 4 2 7 14
Mean Square 22512,203 181,610 1147,715 187,095
F 120,325 ,971 6,134
Sig. ,000 ,480 ,029
a. R Squared = , 992 (Adjusted R Squared = ,984)
142
Lampiran 26. Hasil analisis statistik uji kapasitas antioksidan DPPH semua sampel pada pengenceran 1x dan selang kepercayaan 95%
Univariate Analysis of Variance Between-Subjects Factors N Sampel
Ulangan
1 2 3 4 1 2
2 2 2 2 4 4
Tests of Between-Subjects Effects Dependent Variable: KapAnt iox Source Model Sampel Ulangan Error Total
Ty pe I II Sum of Squares 3561,977a 227,374 ,017 ,013 3561,990
df 5 3 1 3 8
Mean Square 712,395 75,791 ,017 ,004
F 167786,9 17850,719 4,030
Sig. ,000 ,000 ,138
a. R Squared = 1, 000 (Adjusted R Squared = 1,000)
Post Hoc Tests Sampel
143
Multi ple Comparisons Dependent Variable: KapAntiox Tukey HSD
(I) Sampel 1,00
(J) Sampel 2,00 3,00 4,00 1,00 3,00 4,00 1,00 2,00 4,00 1,00 2,00 3,00
2,00
3,00
4,00
Mean Dif f erence (I-J) St d. Error 14,5200* ,06516 4,0750* ,06516 4,7800* ,06516 -14,5200* ,06516 -10,4450* ,06516 -9,7400* ,06516 -4,0750* ,06516 10,4450* ,06516 ,7050* ,06516 -4,7800* ,06516 9,7400* ,06516 -,7050* ,06516
Sig. ,000 ,000 ,000 ,000 ,000 ,000 ,000 ,000 ,005 ,000 ,000 ,005
95% Conf idence Interv al Lower Bound Upper Bound 14,2056 14,8344 3,7606 4,3894 4,4656 5,0944 -14,8344 -14,2056 -10,7594 -10,1306 -10,0544 -9,4256 -4,3894 -3,7606 10,1306 10,7594 ,3906 1,0194 -5,0944 -4,4656 9,4256 10,0544 -1,0194 -,3906
Based on observ ed means. *. The mean dif f erence is signif icant at t he ,05 lev el.
Homogeneous Subsets
KapAntiox a,b
Tukey HSD
Subset Sampel 2,00 4,00 3,00 1,00 Sig.
N 2 2 2 2
1 11,7400
2
3
4
21,4800 22,1850 1,000
1,000
1,000
26,2600 1,000
Means f or groups in homogeneous subsets are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = ,004. a. Uses Harmonic Mean Sample Size = 2,000. b. Alpha = ,05.
144
Lampiran 27. Hasil analisis statistik uji kapasitas antioksidan DPPH semua sampel pada pengenceran 1x dan selang kepercayaan 99%
Univariate Analysis of Variance Between-Subjects Factors N Sampel
Ulangan
1,00 2,00 3,00 4,00 1,00 2,00
2 2 2 2 4 4
Tests of Between-Subjects Effects Dependent Variable: KapAnt iox Source Model Sampel Ulangan Error Total
Ty pe I II Sum of Squares 3561,977a 227,374 ,017 ,013 3561,990
df 5 3 1 3 8
Mean Square 712,395 75,791 ,017 ,004
F 167786,9 17850,719 4,030
Sig. ,000 ,000 ,138
a. R Squared = 1, 000 (Adjusted R Squared = 1,000)
Post Hoc Tests
Sampel
145
Multi ple Comparisons Dependent Variable: KapAntiox Tukey HSD
(I) Sampel 1,00
(J) Sampel 2,00 3,00 4,00 1,00 3,00 4,00 1,00 2,00 4,00 1,00 2,00 3,00
2,00
3,00
4,00
Mean Dif f erence (I-J) St d. Error 14,5200* ,06516 4,0750* ,06516 4,7800* ,06516 -14,5200* ,06516 -10,4450* ,06516 -9,7400* ,06516 -4,0750* ,06516 10,4450* ,06516 ,7050* ,06516 -4,7800* ,06516 9,7400* ,06516 -,7050* ,06516
Sig. ,000 ,000 ,000 ,000 ,000 ,000 ,000 ,000 ,005 ,000 ,000 ,005
99% Conf idence Interv al Lower Bound Upper Bound 13,9593 15,0807 3,5143 4,6357 4,2193 5,3407 -15,0807 -13,9593 -11,0057 -9,8843 -10,3007 -9,1793 -4,6357 -3,5143 9,8843 11,0057 ,1443 1,2657 -5,3407 -4,2193 9,1793 10,3007 -1,2657 -,1443
Based on observ ed means. *. The mean dif f erence is signif icant at t he ,01 lev el.
Homogeneous Subsets
KapAntiox a,b
Tukey HSD
Subset Sampel 2,00 4,00 3,00 1,00 Sig.
N 2 2 2 2
1 11,7400
2
3
4
21,4800 22,1850 1,000
1,000
1,000
26,2600 1,000
Means f or groups in homogeneous subsets are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = ,004. a. Uses Harmonic Mean Sample Size = 2,000. b. Alpha = ,01.
146
Lampiran 28. Hasil analisis statistik pengukuran kadar MDA semua sampel pada pengenceran 1x dan selang kepercayaan 95%
Univariate Analysis of Variance
Between-Subjects Factors N Ulangan Sampel
1 2 11Nov 06 DIPA Kontrol NoLabel
4 4 2 2 2 2
Tests of Between-Subjects Effects Dependent Variable: kadarMDA Source Model Ulangan Sampel Error Total
Ty pe I II Sum of Squares 170545,843a 11,520 3446,080 1243,520 171789,363
df 5 1 3 3 8
Mean Square 34109,169 11,520 1148,693 414,507
F 82,289 ,028 2,771
Sig. ,002 ,878 ,212
a. R Squared = , 993 (Adjusted R Squared = ,981)
147
Lampiran 29. Hasil analisis statistik pengukuran kadar MDA sampel 11 Nov 2006 pada berbagai pengenceran dan selang kepercayaan 95%
Univariate Analysis of Variance Between-Subjects Factors N Ulangan Sampel
1 2 11Nov _1 11Nov _2 11Nov _4
3 3 2 2 2
Tests of Between-Subjects Effects Dependent Variable: kadarMDA Source Model Ulangan Sampel Error Total
Ty pe I II Sum of Squares 40490,726a 34,560 12885,973 31,360 40522,086
df 4 1 2 2 6
Mean Square 10122,682 34,560 6442,987 15,680
F 645,579 2,204 410,905
Sig. ,002 ,276 ,002
a. R Squared = , 999 (Adjusted R Squared = ,998)
Post Hoc Tests
Sampel
148
Multi ple Comparisons Dependent Variable: kadarMDA Tukey HSD
(I) Sampel 11Nov _1
(J) Sampel 11Nov _2 11Nov _4 11Nov _1 11Nov _4 11Nov _1 11Nov _2
11Nov _2 11Nov _4
Mean Dif f erence (I-J) St d. Error 80,40000* 3,959798 109,60000* 3,959798 -80,40000* 3,959798 29,20000* 3,959798 -109,60000* 3,959798 -29,20000* 3,959798
Sig. ,004 ,002 ,004 ,032 ,002 ,032
95% Conf idence Interv al Lower Bound Upper Bound 57,07380 103,72620 86,27380 132,92620 -103,72620 -57,07380 5,87380 52,52620 -132,92620 -86,27380 -52,52620 -5,87380
Based on observ ed means. *. The mean dif f erence is signif icant at t he ,05 lev el.
Homogeneous Subsets
kadarMDA a,b
Tukey HSD Sampel 11Nov _4 11Nov _2 11Nov _1 Sig.
N 2 2 2
1 21,52000
Subset 2
3
50,72000 1,000
1,000
131,12000 1,000
Means f or groups in homogeneous subsets are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = 15,680. a. Uses Harmonic Mean Sample Size = 2,000. b. Alpha = , 05.
149
Lampiran 30. Hasil analisis statistik pengukuran kadar MDA sampel 11 Nov 2006 pada berbagai pengenceran dan selang kepercayaan 99%
Univariate Analysis of Variance Between-Subjects Factors N Sampel
Ulangan
11Nov _1 11Nov _2 11Nov _4 1 2
2 2 2 3 3
Tests of Between-Subjects Effects Dependent Variable: kadarMDA Source Model Sampel Ulangan Error Total
Ty pe I II Sum of Squares 40490,726a 12885,973 34,560 31,360 40522,086
df 4 2 1 2 6
Mean Square 10122,682 6442,987 34,560 15,680
F 645,579 410,905 2,204
Sig. ,002 ,002 ,276
a. R Squared = , 999 (Adjusted R Squared = ,998)
Post Hoc Tests Sampel
150
Multi ple Comparisons Dependent Variable: kadarMDA Tukey HSD
(I) Sampel 11Nov _1
(J) Sampel 11Nov _2 11Nov _4 11Nov _1 11Nov _4 11Nov _1 11Nov _2
11Nov _2 11Nov _4
Mean Dif f erence (I-J) St d. Error 80,40000* 3,959798 109,60000* 3,959798 -80,40000* 3,959798 29,20000 3,959798 -109,60000* 3,959798 -29,20000 3,959798
Sig. ,004 ,002 ,004 ,032 ,002 ,032
99% Conf idence Interv al Lower Bound Upper Bound 27,14642 133,65358 56,34642 162,85358 -133,65358 -27,14642 -24,05358 82,45358 -162,85358 -56,34642 -82,45358 24,05358
Based on observ ed means. *. The mean dif f erence is signif icant at t he ,01 lev el.
Homogeneous Subsets
kadarMDA a,b
Tukey HSD Sampel 11Nov _4 11Nov _2 11Nov _1 Sig.
N 2 2 2
Subset 1 2 21,52000 50,72000 131,12000 ,032 1,000
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = 15,680. a. Uses Harmonic Mean Sample Size = 2,000. b. Alpha = ,01.
151
Lampiran 31. Hasil analisis statistik pengukuran kadar MDA sampel DIPA 14 Juni 2007 pada berbagai pengenceran dan selang kepercayaan 95%
Univariate Analysis of Variance
Between-Subjects Factors N Sampel
Ulangan
DIPA_1 DIPA_2 DIPA_4 1 2
2 2 2 3 3
Tests of Between-Subjects Effects Dependent Variable: kadarMDA Source Model Sampel Ulangan Error Total
Ty pe II I Sum of Squares 35455,262a 12386,210 1276,625 82,850 35538,112
df 4 2 1 2 6
Mean Square 8863,815 6193,105 1276,625 41,425
F 213,972 149,501 30,818
Sig. ,005 ,007 ,031
a. R Squared = , 998 (Adjusted R Squared = ,993)
Post Hoc Tests
Sampel
152
Multi ple Comparisons Dependent Variable: kadarMDA Tukey HSD
(I) Sampel DIPA_1
(J) Sampel DIPA_2 DIPA_4 DIPA_1 DIPA_4 DIPA_1 DIPA_2
DIPA_2 DIPA_4
Mean Dif f erence (I-J) St d. Error 68,80000* 6,436231 110,16000* 6,436231 -68,80000* 6,436231 41,36000* 6,436231 -110,16000* 6,436231 -41,36000* 6,436231
Sig. ,016 ,006 ,016 ,042 ,006 ,042
95% Conf idence Interv al Lower Bound Upper Bound 30,88574 106,71426 72,24574 148,07426 -106,71426 -30,88574 3,44574 79,27426 -148,07426 -72,24574 -79,27426 -3,44574
Based on observ ed means. *. The mean dif f erence is signif icant at t he ,05 lev el.
Homogeneous Subsets
kadarMDA a,b
Tukey HSD Sampel DIPA_4 DIPA_2 DIPA_1 Sig.
N 2 2 2
1 9,76000
Subset 2
3
51,12000 1,000
1,000
119,92000 1,000
Means f or groups in homogeneous subsets are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = 41,425. a. Uses Harmonic Mean Sample Size = 2,000. b. Alpha = ,05.
153
Lampiran 32. Hasil analisis statistik pengukuran kadar MDA sampel DIPA 14 Juni 2007 pada berbagai pengenceran dan selang kepercayaan 99%
Univariate Analysis of Variance Between-Subjects Factors N Ulangan Sampel
1 2 DIPA_1 DIPA_2 DIPA_4
3 3 2 2 2
Tests of Between-Subjects Effects Dependent Variable: kadarMDA Source Model Ulangan Sampel Error Total
Ty pe I II Sum of Squares 35455,262a 1276,625 12386,210 82,850 35538,112
df 4 1 2 2 6
Mean Square 8863,815 1276,625 6193,105 41,425
F 213,972 30,818 149,501
Sig. ,005 ,031 ,007
a. R Squared = , 998 (Adjusted R Squared = ,993)
Post Hoc Tests Sampel Multi ple Comparisons Dependent Variable: kadarMDA Tukey HSD
(I) Sampel DIPA_1 DIPA_2 DIPA_4
(J) Sampel DIPA_2 DIPA_4 DIPA_1 DIPA_4 DIPA_1 DIPA_2
Mean Dif f erence (I-J) St d. Error 68,80000 6,436231 110,16000* 6,436231 -68,80000 6,436231 41,36000 6,436231 -110,16000* 6,436231 -41,36000 6,436231
Sig. ,016 ,006 ,016 ,042 ,006 ,042
99% Conf idence Interv al Lower Bound Upper Bound -17,75804 155,35804 23,60196 196,71804 -155,35804 17,75804 -45,19804 127,91804 -196,71804 -23,60196 -127,91804 45,19804
Based on observ ed means. *. The mean dif f erence is signif icant at t he ,01 lev el.
154
Homogeneous Subsets
kadarMDA a,b
Tukey HSD Sampel DIPA_4 DIPA_2 DIPA_1 Sig.
N 2 2 2
Subset 1 2 9,76000 51,12000 51,12000 119,92000 ,042 ,016
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = 41,425. a. Uses Harmonic Mean Sample Size = 2,000. b. Alpha = ,01.
155
Lampiran 33. Hasil analisis statistik pengukuran kadar MDA sampel No Label 14 Juni 2007 pada berbagai pengenceran dan selang kepercayaan 95%
Univariate Analysis of Variance
Between-Subjects Factors N Sampel
Ulangan
NLabel_1 NLabel_2 NLabel_4 1 2
2 2 2 3 3
Tests of Between-Subjects Effects Dependent Variable: kadarMDA Source Model Sampel Ulangan Error Total
Ty pe II I Sum of Squares 63153,585a 14914,293 1153,707 179,573 63333,158
df 4 2 1 2 6
Mean Square 15788,396 7457,147 1153,707 89,787
F 175,843 83,054 12,849
Sig. ,006 ,012 ,070
a. R Squared = , 997 (Adjusted R Squared = ,991)
Post Hoc Tests
Sampel
156
Multi ple Comparisons Dependent Variable: kadarMDA Tukey HSD
(I) Sampel NLabel_1
(J) Sampel NLabel_2 NLabel_4 NLabel_1 NLabel_4 NLabel_1 NLabel_2
NLabel_2 NLabel_4
Mean Dif f erence (I-J) St d. Error 72,20000* 9,475583 121,40000* 9,475583 -72,20000* 9,475583 49,20000 9,475583 -121,40000* 9,475583 -49,20000 9,475583
Sig. ,031 ,011 ,031 ,063 ,011 ,063
95% Conf idence Interv al Lower Bound Upper Bound 16,38166 128,01834 65,58166 177,21834 -128,01834 -16,38166 -6,61834 105,01834 -177,21834 -65,58166 -105,01834 6,61834
Based on observ ed means. *. The mean dif f erence is signif icant at t he ,05 lev el.
Homogeneous Subsets
kadarMDA a,b
Tukey HSD
Subset Sampel NLabel_4 NLabel_2 NLabel_1 Sig.
N 2 2 2
1 31,72000 80,92000 ,063
2
153,12000 1,000
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = 89,787. a. Uses Harmonic Mean Sample Size = 2,000. b. Alpha = ,05.
157
Lampiran 34. Hasil analisis statistik pengukuran kadar MDA sampel No Label 14 Juni 2007 pada berbagai pengenceran dan selang kepercayaan 99%
Univariate Analysis of Variance
Between-Subjects Factors N Ulangan Sampel
1 2 NL_1 NL_2 NL_4
3 3 2 2 2
Tests of Between-Subjects Effects Dependent Variable: kadarMDA Source Model Ulangan Sampel Error Total
Ty pe I II Sum of Squares 63153,585a 1153,707 14914,293 179,573 63333,158
df 4 1 2 2 6
Mean Square 15788,396 1153,707 7457,147 89,787
F 175,843 12,849 83,054
Sig. ,006 ,070 ,012
a. R Squared = , 997 (Adjusted R Squared = ,991)
Post Hoc Tests
Sampel
158
Multi ple Comparisons Dependent Variable: kadarMDA Tukey HSD
(I) Sampel NL_1
(J) Sampel NL_2 NL_4 NL_1 NL_4 NL_1 NL_2
NL_2 NL_4
Mean Dif f erence (I-J) 72,20000 121,40000 -72,20000 49,20000 -121,40000 -49,20000
St d. Error 9,475583 9,475583 9,475583 9,475583 9,475583 9,475583
Sig. ,031 ,011 ,031 ,063 ,011 ,063
99% Conf idence Interv al Lower Bound Upper Bound -55,23295 199,63295 -6,03295 248,83295 -199,63295 55,23295 -78,23295 176,63295 -248,83295 6,03295 -176,63295 78,23295
Based on observ ed means.
Homogeneous Subsets
kadarMDA a,b
Tukey HSD Sampel NL_4 NL_2 NL_1 Sig.
N 2 2 2
Subset 1 31,72000 80,92000 153,12000 ,011
Means f or groups in homogeneous subset s are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = 89,787. a. Uses Harmonic Mean Sample Size = 2,000. b. Alpha = ,01.
159
Lampiran 35. Inform concern INFORM CONCERN PERNYATAAN KESEDIAAN MENJADI RESPONDEN PENELITIAN Saya yang bertanda tangan di bawah ini:
Nama
: Ari Try Purbayanto
Jenis kelamin
: Laki-laki
Pekerjaan
: Mahasiswa Ilmu dan Teknologi Pangan (ITP)
NRP
: F24052702
Alamat
: Jalan Raya Darmaga Gang Nurul Asman RT 01 RW Darmaga Bogor
Telepon
: 085697486586
Menyatakan dalam keadaan sehat dan bersedia atau tidak berkeberatan untuk dilakukan pengambilan darah untuk keperluan penelitian yang berjudul “Kapasitas Antioksidan, Proliferasi Limfosit dan Hemolisis Eritrosit Manusia Ekstrak Rendang Iradiasi Dosis Tinggi.” Penelitian ini dilakukan dalam rangka penulisan skripsi oleh Kamalita Pertiwi/ NRP F24052300. Pengambilan darah dilakukan di Klinik Farfa Darmaga- Bogor pada bulan Februari 2008 - April 2009. Demikian keterangan ini dibuat untuk digunakan seperlunya.
Petugas Pengambil Darah
Neng
Bogor, Februari 2008 Responden
Ari Try Purbayanto
160
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