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Lampiran 1 Analisis Kandungan klorofil (mg/g berat basah sampel).
Analisis kandungan klorofil a, b, dan klorofil total dilakukan menggunakan metode yang digunakan Richardson et al. (2002) yang merupakan perbaikan metode yang digunakan Arnon (1949) sebagai berikut: - Disiapkan tiga disk sampel daun segar atau 100 mg berat basah, kemudian dimasukkan ke dalam tabung reaksi yang berisi 4.5 ml dimethyl sulfoxide (DMSO). - Sampel tersebut kemudian diinkubasi pada suhu 65oC selama 45 menit, dihindari penguapan untuk mencegah berkurangnya volume ekstrak. - Setelah dingin, larutan ekstrak diukur menggunakan spektrofotometer pada panjang gelombang 645 nm dan 663 nm. - Kandungan klorofil a, klorofil b dan klorofil total dihitung menggunakan rumus Arnon (1949) kemudian dikonversikan menjadi kandungan klorofil daun (mg klorofil/g berat basah sampel), sebagai berikut.
Klorofil A =
(12 .7 A 663 − 2 .69 A 645 ) Volume Berat
Klorofil B =
Klorofil
( 22 . 9 A 645 − 4 . 68 A 663 ) Volume Berat
Total
=
( 20 . 2 A 645 + 8 . 02 A 663 ) Volume Berat
dimana: volume = volume larutan ekstrak DMSO yang digunakan, dan berat = berat basah sampel daun (gram).
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Lampiran 2 Penampilan warna daun beberapa genotipe kedelai pada berbagai intensitas cahaya rendah Genotipe kedelai
Intensitas cahaya rendah Ceneng
Godek
Pangrango
Slamet
Cahaya penuh (L0)
5 hari naungan (L1)
5 hari gelap total (L2)
3 hari naungan + 5 hari cahaya (L3) 3 hari naungan + 3 hari cahaya + 5 hari gelap total (L4)
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Lampiran 3 Diskripsi varietas Pangrango Nomor galur : B8306-4-4 Asal : Persilangan varietas local Lampung x Davros (1983) Warna hipokotil : Ungu Warna bunga : Ungu Warna biji : Kuning Warna hilum biji : Coklat Warna kulit polong masak : Coklat Warna bulu : Coklat Tipe tumbuh : Diterminate Tinggi tanaman : ± 65 cm Jumlah cabang : 3-4 batang Umur mulai berbunga : ± 40 hari Umur polong masak : ± 88 hari Bentuk biji : Bulat – agak bulat Bobot 100 biji : ± 10 gram Ukuran biji : Sedang Kandungan lemak : ± 18% Kandungan protein : ± 39% Hasil pada tumpangsari dengan jagung tertinggi : ± 2.0 ton/ha rata-rata : ± 1.4 ton/ha Ketahanan terhadap penyakit : Tahan karat daun Pemulia : Asadi, Darman M. Arsyad, Sumarno, Hafni Zahara, dan Nurwita Dewi Tahun di lepas : 1995
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Lampiran 4 Diskripsi varietas Slamet Nomor asal : T33 (UNSOED) Asal : Hasil persilangan Dempo x Wilis Warna hipokotil : Ungu Warna epikotil : Ungu Warna daun : Hijau Warna biji : Kuning Warna kulit polong masak : Coklat Warna bulu : Coklat Tipe tumbuh : Diterminate Tinggi tanaman : ± 65 cm Umur mulai berbunga : ± 37 hari setelah tanam Umur polong masak : ± 87 hari setelah tanam Kerabahan : Tahan Bobot 100 biji : ± 12.5 gram Kandungan lemak : ± 15% Kandungan protein : ± 34% Rata-rata hasil : ± 2.26 ton/ha Ketahanan terhadap penyakit : Agak tahan terhadap penyakit karat daun Keterangan : Sesuai untuk tanah masam Pemulia : Sunarto, Noor Farid, dan Suwarto Tahun di lepas : 1995
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Lampiran 5 Sekuen lengkap gen JJ3 di database publik di GenBank LOCUS DEFINITION
EF628505 800 bp mRNA linear PLN 29-MAY-2007 Glycine max photosystem I subunit PsaD (psaD) mRNA, complete cds; chloroplast. ACCESSION EF628505 VERSION EF628505 KEYWORDS . SOURCE chloroplast Glycine max (soybean) ORGANISM Glycine max Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids I; Fabales; Fabaceae; Papilionoideae; Phaseoleae; Glycine. REFERENCE 1 (bases 1 to 800) AUTHORS Khumaida,N., Sopandie,D. and Takano,T. TITLE Shade tolerance-related gene on soybean (Glycine max): homolog to psaD gene of PSI subunit JOURNAL Unpublished REFERENCE 2 (bases 1 to 800) AUTHORS Khumaida,N., Sopandie,D. and Takano,T. TITLE Direct Submission JOURNAL Submitted (21-MAY-2007) Agronomy and Horticulture, Faculty Of Agriculture, Bogor Agricultural University (IPB), Jl. Meranti, Kampus IPB Darmaga, Bogor, West Java 16680, Indonesia FEATURES Location/Qualifiers source 1..800 /organism="Glycine max" /organelle="plastid:chloroplast" /mol_type="mRNA" /db_xref="taxon:3847" gene 1..800 /gene="psaD" /note="gmpsaD" CDS 27..659 /gene="psaD" /note="PS-1 subunit; transport electron PSI; PsaD protein" /codon_start=1 /transl_table=11 /product="photosystem I subunit PsaD" /translation="MAMATQASLLTPPLSGLKASDRASVPWKQNSSLSF SSPKPLKFSRTIRAAAADETTEAPAKVEAAPVGFTPPELDPNTPSPIF GGSTGGLLRKAQVEEFYVITWDSPKEQIFEMPTGGAAIMREGPNLLKL ARKEQCLALGTRLRSKYKIKYQFYRVFPNGEVQYLHPKDGVYPEKVNA GRQGVGQNFRSIGKNVSPIEVKFTGKQPYDL" ORIGIN 1 gaacacttgt attatctcaa gcaaccatgg caatggcaac ccaagcctct ctcttaaccc 61 cacccctctc cggtctcaaa gccagcgacc gcgcctccgt gccatggaag caaaactcca 121 gcctctcctt ctccagcccg aagcccctca agttctccag aacaatcaga gcagcagccg 181 ccgacgagac cacagaggca ccagcaaaag tagaggctgc accggtcggg ttcaccccac 241 cagaacttga cccaaacacc ccttccccga tcttcggggg cagcaccggc gggctcctgc 301 gcaaggcaca ggtggaggag ttttatgtca ttacgtggga ctcacccaaa gaacagatct 361 ttgaaatgcc cactggcggc gccgctatca tgagggaggg tcctaacctt ctcaagttgg 421 ccaggaagga gcagtgcttg gctcttggga ctaggctcag gtccaagtac aagatcaagt 481 accagttcta cagggtcttc cctaatgggg aggttcagta tttgcaccct aaggatggtg 541 tttaccctga gaaggtcaac gccggacgcc aaggggtggg tcaaaacttc aggtctattg 601 gtaagaatgt tagtcctatt gaggtcaagt tcactggcaa gcagccctat gatttgtgag 661 cacacaactc tatcttcatc atcatcatcc cccgtgcttc ctttatatgc tatatattct 721 catgtgatat catgtaccta ttgtcaattt tattatgcca caaatattgc taaaaaaaaa 781 aaaaaaaaaa aaaaaaaaaa //
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