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"------M 1 f. I K ERPUSTAKAAN RSITAS AIRLANGOA
URA BA YA
DEWI KUMALAYANTI
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JURUSAN BIOLOGI FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM ·_ ·~. , UNIVERSITAS AIRLANGGA ....i) ;~ ~: SURABAYA -i: ~- . ..'!
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PENGARUH INDUKSI 2-METHOXYETHANOL (2-ME) P ADA EMBRIO MENCIT (Mus musculus) TAHAP 2 SEL TERHADAP KEMAMPUAN PROSES IMPLANTASI DAN PERKEMBANGAN EMBRIO POST IMPLANTASI
SKRIPSI Sebagai Salah Satu Syarat Untuk Memperoleh Gelar Sarjana Sains Bidang Biologi Pada Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Airlangga
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Oleh:
Mll, l'K
PERPUST AKA AN UN IVERSlTAS AIRLANGGA
\
DEWI KUM..~AYANTI
bU l{ A BA YA
NIM : 089711635
Tanggal Lulus Ujian : 19 Juli 2002 Disetujui Oleh : Pembimbing I,
Pembimbing I,
Drs. Win Dannanto, M. S., Ph. D NIP. 131 653 741
Drs. Eko Prlhiyantoro, M. Kes NIP. 132 049 477
LEMBAR PENGESAHAN SKRIPSI : Pengaruh Induksl 2-Methoxyetluawl (2-ME)
Judul
Pada
Embrio Mencit (MllS musculus) Tahap 2 Sel Terhadap Kemampuan Proses Implantasl dan Perkembangan Embrio Post hnplantasi Penyusun
: Dewl KumalayanU
NIM
: 089711635
Tanggal Ujian
: 19 Juli 2002
Disetujui Oleh : Pembimblng I,
Ors. Eko Priltlyantoro. M. Kes. NIP. 132 049 477
Drs. Win Darmanto, M. S., Ph. D NIP. 131653741
Mengetahul : KETIJA JUR SAN BIOLOGI FM.IPA UNIVERSITAS AIRLANGGA
Dra. Rosmanida, M.Kes. NIP. 131126 075
LEMBAR PENGESAHAN UJIAN SKRIPSI Judul
: Pengaruh Indukst 2-Metlwxyetlumol (2-ME)
Pada
Embrto Mencit (Jt,fus musculus) Tahap 2 Sel T erhadap Kemampuan Proses Implantasi dan Perkembangan Embrto Post Implantasi Penyusun
: Dewl KumalayanU
NIM
089711635
Tanggal Ujtan
19 Juli 2002
Naskah Skripsi ini telah diperbaiki sesuai dengan saran-saran dalmn forum ujian
Disetujut Oleh :
Pengujl I,
Drs. Win Darmanto, M. S., Ph. D NIP. 131 653 741
Drs. Eko Prihtyantoro, M. Kes NIP. 132 049 477
PenguJI IV, \
s. J.B. Rai Pidada, M.Si NIP. 130 531 824
Dra. Rosmanida, M.Kes NIP. 131126 075
Dewi Kurnalayanti. 2002. Pengaruh Induksi 2-Methoxyethanol (2-ME) Pada Embrio Mencit (Mus musculus) Tahap 2 Sel Terhadap Kemampuan Proses lmplantasi dan Perkembangan Embrio Post Implantasi. Skripsi ini di bawah bimbingan Drs. Win Darmanto, M.S., Ph.D dan Drs. Eko Prihiyantoro, M.Kes. Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Airlangga Surabf}ya.
ABSTRAK Tujuan
Kata kunci : 2-M{!thoxyethanol, mencit, implantasi, endometriurn Vll
Dewi Kumalayanti. 2002. Effects of 2-Methoxyethanol (2-ME) on The Process of Implantation and Post Implantation Embryo Following Two-Cell Stage of Embryo Exposure. This thesis written under the advisory of Ors. Win Darmanto, M.S., Ph.D aI\d Ors. Eko Prihiyantoro, M.Kes. Department of Biology, Mathematic and Science Faculty, Airlangga University Surabaya.
ABSTRACT 2-Metl1o)f.yethanol (2-ME) as a pollutan in the environment and has been known as a male or female reproductive disrnptor, however effects of 2-ME in the implantation pro~ess is not documented yet. This research was designed, how the effects of 2-ME on the process of implantation and post implantation embryos following two-cell stage embryo exposure. Pregnant mice on gestational day (GD) 0 (embryo two-cell stage) was injected intrapefitoneally 2-ME at dose of 10 mmol/kg body weight. The condi~ion of endometrium were observed on GD 5 when implantation process was occurs. The via,0ility of implanted embrios were observed on GD 18. The condition of enqometrium including thickness of endometrium, number of blood vessels, and nu.rµber of gland tubules were observed, the data were analyzed by t-test. The thicl5:.ness of endometrium and the number of gland tubules tended decreased, however the number of blood vessels tended increased. Whereas no significantly diff~rent between the number of implantation on the treatment and control groups. However the number of live fetuses tended decreased and also total of intrauterj.ne death tended increased. There were also tended decreased in the body weight of live fetuses and the incident of external malformations tended increased. These result indicate that 2-ME has no significantly effects in the development of endometrium and embryo post implantation when exposed on GD 0 at dose 10 ,mmol/kg body weight.
Key words : 2-M~thoxyethanol , mice, implantation, endometrium.
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