Lampiran A. Data Pengamatan Berat Testis Mencit

Lampiran A. Data Pengamatan Berat Testis Mencit a. Data Pengamatan Berat Testis Mencit Waktu Pemberian

Minggu ke-0

Minggu ke-6

Minggu ke-12

Minggu ke-18

Minggu ke-24

Ulangan

Data Berat Testis (mg) K

P

1 2 3 4 5 Rata-Rata

95 200 150 165 95 141

150 110 70 70 80 96

1 2 3 4 5 Rata-Rata

85 100 165 70 105 105

95 100 155 110 110 114

1 2 3 4 5 Rata-Rata

80 60 120 40 85 77

80 120 0 80 95 375

1 2 3 4 5 Rata-Rata

115 95 140 150 175 135

45 100 100 155 0 80

1 2 3 4 5 Rata-Rata

110 125 145 140 145 133

150 0 0 60 65 55

Universitas Sumatera Utara

b. Tabel Rata-Rata Berat Testis Mencit Setelah Pemberian Kombinasi Ekstrak Air Biji Pepaya (Carica papaya L.) dan Testosteron Undekanoat (TU). Kelompok (Minggu) K0 (0 minggu) K1 (6 minggu) K2 (12 minggu) K3 (18 minggu) K4 (24 minggu)

Kontrol

Perlakuan

141 ± 45.74 105 ± 36.23 77 ± 29.92 135 ± 31.02 133 ± 15.25

96 ± 34.35 114 ± 23.82 75 ± 45.0 80 ± 59.27 55 ± 61.64

c. Analisis Statistik Tests of Normality Kolmogorov-Smirnova KELOMPOK DATA_BERAT

Statistic

df

Shapiro-Wilk

Sig.

Statistic

Df

Sig.

KONTROL

.112

25

.200*

.987

25

.978

PERLAKUAN

.121

25

.200*

.919

25

.048

a. Lilliefors Significance Correction *. This is a lower bound of the true significance.

Independent Samples Test Levene's Test for Equality of Variances

t-test for Equality of Means 95% Confidence Interval of the Difference

F DATA_BERAT Equal variances assumed Equal variances not assumed

.390

Sig.

t

Sig. (2Mean Std. Error tailed) Difference Difference Lower

df

Upper

.535 2.782

48

.008 34.20000 12.29458 9.48009 58.91991

2.782

46.218

.008 34.20000 12.29458 9.45545 58.94455

Universitas Sumatera Utara

Tests of Normality Kolmogorov-Smirnova KELOMPOK K0P0

Statistic

KONTROL

df

.243

Shapiro-Wilk

Sig. 5

Statistic

df

Sig.

*

.898

5

.397

*

.836

5

.155

.200

.279

5

K1P1

PERLAKUAN KONTROL

.300

5

.161

.880

5

.309

.367

5

.027

.788

5

.064

K2P2

PERLAKUAN KONTROL

.195

5

*

.200

.979

5

.930

K3P3

PERLAKUAN KONTROL

.344 .164

5 5

.053 .200*

.858 .986

5 5

.222 .965

.232

5

.200*

.963

5

.826

K4P4

PERLAKUAN KONTROL

5

*

.848

5

.190

*

.876

5

.291

.277

PERLAKUAN

.236

.200

.200

5

.200

a. Lilliefors Significance Correction *. This is a lower bound of the true significance.

Independent Samples Test Levene's Test for Equality of Variances

F K0P0

Equal variances assumed

.704

Sig.

t-test for Equality of Means

t

.426 1.759

Equal variances not assumed

Sig. (2Mean Std. Error tailed) Difference Difference

df

95% Confidence Interval of the Difference Lower

Upper

8

.117 45.00000 25.58320 -13.99496

103.99496

1.759 7.423

.120 45.00000 25.58320 -14.80299

104.80299

Mann-Whitney Test Test Statisticsb

Ranks KELOMPOK K1P1 KONTROL PERLAKUAN Total

Mean Rank

N

Sum of Ranks

5

4.70

23.50

5

6.30

31.50

10

K1P1 Mann-Whitney U Wilcoxon W Z

8.500 23.500 -.841

Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]

.401 a

.421

a. Not corrected for ties. b. Grouping Variable: KELOMPOK

Universitas Sumatera Utara

Independent Samples Test Levene's Test for Equality of Variances

F K2P2

Equal variances assumed

.291

Sig. .604

Equal variances not assumed

t-test for Equality of Means

t

Sig. (2Mean Std. Error tailed) Difference Difference

df

.083

95% Confidence Interval of the Difference Lower

Upper

8

.936

2.00000 24.16609

-53.72711

57.72711

.083 6.958

.936

2.00000 24.16609

-55.21375

59.21375

Independent Samples Test Levene's Test for Equality of Variances

F K3P3

Equal variances assumed

2.180

Sig.

t-test for Equality of Means

t

.178 1.838

Equal variances not assumed

Sig. (2Mean Std. Error tailed) Difference Difference

df

95% Confidence Interval of the Difference Lower

Upper

8

.103 55.00000 29.91655

-13.98769

123.98769

1.838 6.039

.115 55.00000 29.91655

-18.08854

128.08854

Independent Samples Test Levene's Test for Equality of Variances

F K4P4

Equal variances assumed Equal variances not assumed

3.515

Sig.

t-test for Equality of Means

t

.098 2.747

Sig. (2Mean Std. Error tailed) Difference Difference

df

95% Confidence Interval of the Difference Lower

Upper

8

.025 78.00000 28.39894

12.51192

143.48808

2.747 4.488

.045 78.00000 28.39894

2.41867

153.58133

Universitas Sumatera Utara

Lampiran B. Data Pengamatan Volume Testis Mencit a. Data Pengamatan Berat Testis Mencit Pemberian Kombinasi

Ulangan

Minggu ke-0

1 2 3 4 5 Rata-Rata

Data Volume Testis (cm³) K P 178.98 141.3 169.56 113.04 141.3 160.14 141.3 118.27 160.14 141.3 158.25 134.81

Minggu ke-6

1 2 3 4 5 Rata-Rata

178.98 141.3 113.04 98.08 169.56 140.19

141.3 113.04 141.3 150.72 113.04 131.88

Minggu ke-12

1 2 3 4 5 Rata-Rata

178.98 169.56 113.04 98.08 113.04 134.54

127.69 141.3 0 150.72 131.88 110.318

Minggu ke-18

1 2 3 4 5 Rata-Rata

141.3 150.72 160.14 160.14 160.14 154.488

78.5 127.69 118.27 113.04 98.08 107.16

Minggu ke-24

1 2 3 4 5 Rata-Rata

141.3 160.14 150.19 169.56 169.56 158.15

141.3 141.3 150.72 130.83 113.04 135.43

Universitas Sumatera Utara

b. Tabel Rata-Rata Volume Testis Mencit Setelah Pemberian Kombinasi Ekstrak Air Biji Pepaya (Carica papaya L.) dan Testosteron Undekanoat (TU). Kelompok (Minggu) K1 (0 minggu) K2 (6 minggu) K3 (12 minggu) K4 (18 minggu) K5 (24 minggu)

Kontrol

Perlakuan

158.25 ± 16.85 140.19 ± 34.92 134.54 ± 36.93 154.48 ± 8.43 158.15 ± 12.37

134.81 ± 19.19 131.88 ± 17.62 110.32 ± 62.31 107.16 ± 19.26 135.43 ± 14.36

c. Analisis Statistik Tests of Normality Kolmogorov-Smirnova KELOMPOK

Statistic

DATA_VOLUME KONTROL PERLAKUAN

df

Shapiro-Wilk

Sig.

Statistic

df

Sig.

.191

25

.019

.884

25

.009

.247

25

.000

.742

25

.000

a. Lilliefors Significance Correction Test Statisticsa

Mann-Whitney Test Ranks KELOMPOK DATA_ KONTROL VOLUME PERLAKUAN Total

N

Mean Sum of Rank Ranks

25 32.00 800.00 25 19.00 475.00 50

DATA_VOLUME Mann-Whitney U Wilcoxon W Z Asymp. Sig. (2-tailed)

150.000 475.000 -3.187 .001

a. Grouping Variable: KELOMPOK

Friedman Test Test Statisticsa

Ranks Mean Rank K0 K1 K2

3.40 2.50 2.50

K3 K4

3.00 3.60

N Chi-Square Df Asymp. Sig.

5.000 2.267 4.000 .687

a. Friedman Test

Universitas Sumatera Utara

Test Statisticsa

Ranks Mean Rank P0 P1 P2 P3 P4

3.70 3.50 3.40 2.00 2.40

N

5.000

Chi-Square df Asymp. Sig.

4.913 4.000 .296

a. Friedman Test

Tests of Normality Kolmogorov-Smirnova KELOMPOK K0P0

Statistic

KONTROL_VOLUME

df

.243

Shapiro-Wilk

Sig.

Statistic

df

Sig.

5

.200*

.894

5

.377

5

*

.922

5

.545

*

.931

5

.606

.232

K1P1

PERLAKUAN_VOLUME KONTROL_VOLUME

.200

5

.304

5

.149

.817

5

.111

K2P2

PERLAKUAN_VOLUME KONTROL_VOLUME

.320

5

.105

.833

5

.147

.410

5

.006

.689

5

.007

K3P3

PERLAKUAN_VOLUME KONTROL_VOLUME

.349

5

.046

.771

5

.046

5

*

.951

5

.747

*

.900

5

.408

*

.921

5

.538

K4P4

PERLAKUAN_VOLUME KONTROL_VOLUME PERLAKUAN_VOLUME

.221 .222

.200 .200

.200

5

.258

.200

5

.200

a. Lilliefors Significance Correction *. This is a lower bound of the true significance. Independent Samples Test Levene's Test for Equality of Variances

F K0P0

Equal variances assumed

Sig.

Equal variances assumed Equal variances not assumed

t

.120 .738 2.053

Equal variances not assumed K1P1

t-test for Equality of Means

2.782 134

Sig. (2tailed)

df

Mean Std. Error Difference Difference

95% Confidence Interval of the Difference Lower

Upper

8

.074

23.44600 11.42186

-2.89287 49.78487

2.053 7.868

.075

23.44600 11.42186

-2.96978 49.86178

.475

8

.647

8.31200 17.49454 -32.03048 48.65448

.475 5.913

.652

8.31200 17.49454 -34.64871 51.27271

Universitas Sumatera Utara

Mann-Whitney Test Ranks KELOMPOK K2P2

Mean Rank

N

KONTROL

Test Statisticsb

5

PERLAKUAN

5.60

5

Total

K2P2

Sum of Ranks

5.40

Mann-Whitney U

12.000

28.00

Wilcoxon W

27.000

27.00

Z

10

-.105

Asymp. Sig. (2-tailed)

.917 1.000a

Exact Sig. [2*(1-tailed Sig.)] a. Not corrected for ties. b. Grouping Variable: KELOMPOK

Test Statisticsb

Ranks KELOMPOK

Mean Rank

N

K3P3 KONTROL PERLAKUAN Total

Sum of Ranks

K3P3 Mann-Whitney U

.000

5

8.00

40.00

Wilcoxon W

15.000

5

3.00

15.00

Z

-2.643

Asymp. Sig. (2-tailed)

10

.008 .008a

Exact Sig. [2*(1-tailed Sig.)] a. Not corrected for ties. b. Grouping Variable: KELOMPOK

Independent Samples Test Levene's Test for Equality of Variances

F K4P4

Equal variances assumed Equal variances not assumed

.043

Sig.

t-test for Equality of Means

t

.841 2.680

Sig. (2Mean tailed) Difference

df

Std. Error Difference

95% Confidence Interval of the Difference Lower

Upper

8

.028 22.71200

8.47582

3.16673 42.25727

2.680 7.827

.028 22.71200

8.47582

3.09132 42.33268

Universitas Sumatera Utara

Lampiran C. Data Pengamatan Diameter Tubulus Seminiferus Testis Mencit a. Data Pengamatan Diameter Tubulus Seminiferus Testis Mencit Pemberian Kombinasi

Ulangan

Minggu ke-0

1 2 3 4 5 Rata-Rata

Data Diameter Tubulus Seminiferus (µ) K P 184.23 91.37 171.94 194.94 128.49 93.59 91.97 117.19 100.19 92.92 135.36 118.00

Minggu ke-6

1 2 3 4 5 Rata-Rata

163.38 81.00 186.97 87.25 91.49 122.02

179.72 150.19 98.55 215.50 115.59 151.91

Minggu ke-12

1 2 3 4 5 Rata-Rata

131.94 105.14 62.89 99.42 112.31 102.34

129.19 93.64 0.00 79.42 87.43 77.94

Minggu ke-18

1 2 3 4 5 Rata-Rata

84.02 96.64 102.29 94.41 107.86 97.04

86.42 87.38 98.05 111.16 0.00 76.60

Minggu ke-24

1 2 3 4 5 Rata-Rata

215.55 259.70 119.58 176.56 130.41 180.36

87.97 0.00 0.00 78.70 72.67 47.87

Universitas Sumatera Utara

b. Rata-Rata Diameter Kombinasi Ekstrak Undekanoat (TU). Kelompok (Minggu) K1 (0 minggu) K2 (6 minggu) K3 (12 minggu) K4 (18 minggu) K5 (24 minggu)

Tubulus Seminiferus Testis Mencit Setelah Pemberian Air Biji Pepaya (Carica papaya L.) dan Testosteron Kontrol

Perlakuan

135.36 ± 41.51 122.02 ± 49.38 102.34 ± 25.24 97.04 ± 8.96 180.36 ± 58.60

118 ± 44.31 151.91 ± 47.40 77.94 ± 47.54 76.6 ± 43.97 47.87 ± 44.04

c. Analisis Statistik Tests of Normality Kolmogorov-Smirnova KELOMPOK

Statistic

DATA_DIAMETER KONTROL PERLAKUAN

df

Sig.

Shapiro-Wilk Statistic

Df

Sig.

.183

25

.030

.883

25

.008

.189

25

.022

.902

25

.020

a. Lilliefors Significance Correction Mann-Whitney Test Ranks

DATA_ DIAMETER

Test Statisticsa Mean Sum of Rank Ranks

KELOMPOK

N

KONTROL

25 30.08

752.00

PERLAKUAN

25 20.92

523.00

Total

50

DATA_DIAMETER Mann-Whitney U Wilcoxon W Z Asymp. Sig. (2-tailed)

198.000 523.000 -2.222 .026

a. Grouping Variable: KELOMPOK Friedman Test Test Statisticsa

Ranks Mean Rank K0 K1 K2 K3 K4

3.20 2.20 2.80 2.20 4.60

N

5.000

Chi-Square Df

7.840 4.000

Asymp. Sig.

.098

Universitas Sumatera Utara

Rank Test Statisticsa

Mean Rank P0 P1 P2 P3 P4

3.80 4.80 2.70 2.20 1.50

N Chi-Square

5.000 13.859

Df Asymp. Sig.

4.000 .008

a. Friedman Test

Wilcoxon Signed Ranks Test Test Statisticsc P1 - P0 P2 - P0 P3 - P0 P4 - P0 P2 - P1 P3 - P1 P4 - P1 P3 - P2 P4 - P2 P4 - P3 Z

-1.214a -1.214b -1.753b -2.023b -2.023b -2.023b -2.023b

Asymp. Sig. (2tailed)

.225

.225

.080

.043

.043

.043

.043

-.135b -1.826b .893

.068

-.944b .345

a. Based on negative ranks. b. Based on positive ranks. c. Wilcoxon Signed Ranks Test

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Lampiran D. Data Pengamatan Luas Lapisan Germinal Tubulus Seminiferus Testis Mencit a. Data Pengamatan Luas Lapisan Germinal Tubulus Seminiferus Testis Mencit Data Luas Lapisan Germinal Pemberian Ulangan Tubulus Seminiferus (µ²) Kombinasi K P 33961.37 1 23896.70 2 26169.19 28100.24 3 26978.99 24343.53 Minggu ke-0 4 30549.01 26898.99 5 18984.65 19645.83 Rata-Rata 27328.64 24577.06

Minggu ke-6

1 2 3 4 5 Rata-Rata

30951.46 20958.51 24835.33 20755.45 26007.54 123508.29

29881.09 33659.22 23506.50 40932.00 31549.37 31905.64

Minggu ke-12

1 2 3 4 5 Rata-Rata

31741.07 30324.13 10543.00 24038.60 31416.77 25612.71

27125.67 23874.25 0.00 12962.98 16893.41 16171.26

Minggu ke-18

1 2 3 4 5 Rata-Rata

22760.59 10084.04 25046.37 26548.71 27226.15 22333.17

24440.89 20755.85 22081.70 32119.23 0.00 19879.53

Minggu ke-24

1 2 3 4 5 Rata-Rata

115873.75 38341.16 30526.99 35727.66 27463.67 49586.65

21759.91 0.00 0.00 14267.62 14263.46 10058.20

Universitas Sumatera Utara

b. Rata-Rata Luas Lapisan Germinal Tubulus Seminiferus Testis Mencit Setelah Pemberian Kombinasi Ekstrak Air Biji Pepaya (Carica papaya L.) dan Testosteron Undekanoat (TU). Kelompok (Minggu) K1 (0 minggu) K2 (6 minggu) K3 (12 minggu) K4 (18 minggu) K5 (24 minggu)

Kontrol

Perlakuan

27328.64 ± 5600.28 24701.66 ± 4194.25 25612.71 ± 8986.31 22333.17 ± 7058.64 49586.65 ± 37300.2

24577.06 ± 3264.91 31905.64 ± 6311.13 16171.26 ± 10626.14 99397.67 ± 11952.3 10058.2 ± 9678.17

c. Analisis Statistik Tests of Normality Kolmogorov-Smirnova KELOMPOK DATA_LUAS

Statistic

df

Shapiro-Wilk

Sig.

Statistic

df

Sig.

KONTROL

.302

25

.000

.528

25

.000

PERLAKUAN

.149

25

.159

.915

25

.040

a. Lilliefors Significance Correction Mann-Whitney Test Ranks

DATA_ LUAS

DATA_LUAS Mean Sum of Rank Ranks

KELOMPOK

N

KONTROL

25 29.20

730.00

PERLAKUAN

25 21.80

545.00

Total

50

Mann-Whitney U Wilcoxon W Z Asymp. Sig. (2-tailed)

220.000 545.000 -1.796 .073

a. Grouping Variable: KELOMPOK

Test Statisticsa

Universitas Sumatera Utara

Tests of Normality Kolmogorov-Smirnova KELOMPOK

Statistic

df

Shapiro-Wilk

Sig.

Statistic

df

Sig.

K0P0

KONTROL

.211

5

.200*

.897

5

.394

.309

5

.133

.710

5

.012

K1P1

PERLAKUAN KONTROL

.332

5

.075

.809

5

.095

5

*

.967

5

.854

*

.942

5

.683

PERLAKUAN K2P2

.178

.200

KONTROL

.254

5

PERLAKUAN

.312

5

.124

.882

5

.319

*

.980

5

.937

.200

K3P3

KONTROL

.184

5

.388

5

.013

.760

5

.037

K4P4

PERLAKUAN KONTROL

.203

5

.200*

.941

5

.671

PERLAKUAN

.313

5

.122

.774

5

.049

.200

a. Lilliefors Significance Correction *. This is a lower bound of the true significance. Independent Samples Test Levene's Test for Equality of Variances

F K0P0

Equal variances assumed

.055

Sig. .820

Equal variances not assumed K2P2

Equal variances assumed Equal variances not assumed

.765

t-test for Equality of Means

T

Sig. (2Mean tailed) Difference

df

Std. Error Difference

95% Confidence Interval of the Difference Lower

Upper

8

.540 17.36200

27.15476

79.98099 45.25699

.639 7.966

.541 17.36200

27.15476

80.02733 45.30333

.639

.407 1.014

8

.340

24.40400

24.07091 -31.10362 79.91162

1.014

6.089

.349

24.40400

24.07091 -34.28714 83.09514

Universitas Sumatera Utara

Mann-Whitney Test Ranks KELOMPOK K3P3

Mean Rank

N

Sum of Ranks

KONTROL

5

6.00

30.00

PERLAKUAN

5

5.00

25.00

Total

10

b

Test Statistics

k3p3 Mann-Whitney U

10.000

Wilcoxon W

25.000

Z Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]

-.522 .602 .690a

a. Not corrected for ties. b. Grouping Variable: KELOMPOK

Universitas Sumatera Utara

Independent Samples Test Levene's Test for Equality of Variances

F K4P4

Equal variances assumed Equal variances not assumed

.308

Sig.

t-test for Equality of Means

t

.594 4.042

Sig. (2- Mean Std. Error tailed) Difference Difference

df

95% Confidence Interval of the Difference Lower

Upper

8

.004 132.49200 32.78219 56.89614

208.08786

4.042 7.425

.004 132.49200 32.78219 55.86527

209.11873

Universitas Sumatera Utara

Lampiran E. Pembuatan Ekstrak Biji Pepaya Buah Pepaya

Diambil biji pepaya Dikeringkan dalam inkubator pada suhu 500 Celsius Dihaluskan hingga menjadi serbuk Diambil 300 g dan dimasukkan kedalam bejana berisi 1000 mL air Dipanaskan di atas hotplate Disaring dan diambil filtratnya Dipanaskan filtrat di atas hotplate hingga diperoleh cairan kental berwarna hitam Diambil 30 g ekstrak kental dan dilarutkan dalam 500 mL aquabidestilata

Hasil

Universitas Sumatera Utara

Lampiran F. Pembuatan Preparat Histologis Testis

Testis Dibilas dengan NaCl 0,9% Difiksasi dalam BOUIN selama 1 malam Washing dengan alkohol 70% Dehidrasi dalam alkohol konsentrasi bertingkat mulai 70%, 80%, 96% hingga 100% Clearing dalam xylol Infiltrasi Embedding (penanaman) organ dalam cetakan kemudian dituangkan parafin murni, dibiarkan hingga didapatkan blok parafin Blok Parafin Cutting (pemotongan) menggunakan mikrotum sehingga didapatkan pita-pita parafin Blok Parafin Attaching (Penempelan) pita parafin pada gelas objek Deparafinasi dengan mencelupkan objek dalam xylol Dealkoholisasi dalam konsentrasi menurun dari 100%, 96%, 80% hingga 70% Pewarnaan dengan mencelupkan dalam Hematoxilin selama 3-7 menit kemudian dibilas dengan air mengalir. Selanjutnya dicelupkan dalam alkohol 70%, lalu dicelupkan dalam Eosin selama 1-3 menit. Mounting yaitu menutup preparat dengan gelas penutup yang sebelumnya diberi Canada balsam Diberi label dan siap diamati Hasil

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Lampiran G. Dokumentasi Gambar Histologi Testis Mencit

K0

Histologi Testis Kelompok Kontrol Minggu-0 (10x10)

P0

Histologi Testis Kelompok Perlakuan Minggu-0 (10x10)

Universitas Sumatera Utara

K1

Histologi Testis Kelompok Kontrol Minggu-6 (10x10)

P1

Histologi Testis Kelompok Perlakuan Minggu-6 (10x10)

Universitas Sumatera Utara

K2

Histologi Testis Kelompok Kontrol Minggu-12 (10x10)

P2

Histologi Testis Kelompok Perlakuan Minggu-12 (10x10)

Universitas Sumatera Utara

K3 Histologi Testis Kelompok Kontrol Minggu-18 (10x10)

P3

Histologi Testis Kelompok Perlakuan Minggu-18 (10x10)

Universitas Sumatera Utara