LAMPIRAN 1 PERHITUNGAN DOSIS
Berdasarkan jurnal “A Dose-Response Study on the Effects of Purified Lycopene Supplementation on Biomarkers of Oxidative Stress”, disebutkan bahwa dosis likopen 30 mg/hari pada manusia dapat menurunkan kerusakan akibat stres oksidatif (Devaraj et al, 2008). Sediaan likopen yang digunakan berupa pasta tomat. Menurut data USDA (1998) dalam 100 gram pasta tomat terdapat 29,33 mg likopen.
Dosis pasta tomat untuk manusia: 30 mg x 100 gram = 102,28 gram 29,33 mg
Dosis pasta tomat untuk mencit (berat badan 20 gram) = 102,28 gram x 0,0026 (faktor konversi dari manusia ke mencit) = 0,27 gram
Dosis pasta tomat untuk mencit berat badan 24 gram 24 gram x 0,27 gram = 0,324 gram = 0,32 gram 20 gram Pasta tomat dosis I: ½ x 0,32 gram = 0,16 gram pasta tomat Pasta tomat dosis II: 1 x 0,32 gram = 0,32 gram pasta tomat Pasta tomat dosis III: ¾ x 0,32 gram = 0,48 gram pasta tomat
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LAMPIRAN 2 KADAR LIKOPEN PADA BERBAGAI PRODUK TOMAT
Product
Lycopene
Serving
Lycopene
(mg /100 g)
Size
(mg /serving)
9.32
240 mL (1 cup)
22.9
Tomato Ketchup
17.01
15 mL (1 tbsp)
2.89
Spaghetti Sauce
15.99
125 g (1/2 cup)
19.99
Tomato Paste
29.33
30 g (2 tbsp)
8.80
Tomato Soup
10.92
245 g (1 cup
13.10
Tomato Juice (without salt added)
(Condensed)
prepared)
Tomato Sauce
15.92
60 g (1/4 cup)
9.55
Raw Tomato
3.0
148 g (1 medium)
4.5
(USDA-NCC carotenoid database for U.S. foods-1998)
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LAMPIRAN 3 TABEL PENGAMATAN HASIL PENELITIAN
Tabel Diameter Tubulus Seminiferus Kelompok Kontrol Akuades 1 Tubulus 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Ratarata
X
Sok 15 13 15 15 16 15 16 14 14 15 14 15 15 13 17 15 15 15 14 13 19 13 16 15 15
µm 200,0 187,5 162,5 212,5 175,0 187,5 200,0 162,5 162,5 200,0 175,0 187,5 187,5 212,5 187,5 200,0 187,5 175,0 200,0 212,5 187,5 187,5 187,5 212,5 212,5
Mencit 3 X Sok µm 12 150,0 11 137,5 12 150,0 12 150,0 12 150,0 13 162,5 11 137,5 12 150,0 10 125,0 12 150,0 12 150,0 11 137,5 12 150,0 14 175,0 10 125,0 11 137,5 11 137,5 13 162,5 12 150,0 12 150,0 12 150,0 11 137,5 11 137,5 13 162,5 12 150,0
190,5
147,0
2 µm 187,5 162,5 187,5 187,5 200,0 187,5 200,0 175,0 175,0 187,5 175,0 187,5 187,5 162,5 212,5 187,5 187,5 187,5 175,0 162,5 237,5 162,5 200,0 187,5 187,5 186,0
X
Sok 16 15 13 17 14 15 16 13 13 16 14 15 15 17 15 16 15 14 16 17 15 15 15 17 17
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4 X
Sok 12 12 12 12 12 13 12 11 13 14 12 13 11 11 14 13 13 12 13 13 15 14 12 12 12
5 µm 150,0 150,0 150,0 150,0 150,0 162,5 150,0 137,5 162,5 175,0 150,0 162,5 137,5 137,5 175,0 162,5 162,5 150,0 162,5 162,5 187,5 175,0 150,0 150,0 150,0 156,5
X
Sok 15 17 18 15 15 14 15 14 11 16 16 13 13 15 19 14 16 15 15 13 17 14 17 13 13
µm 187,5 212,5 225,0 187,5 187,5 175,0 187,5 175,0 137,5 200,0 200,0 162,5 162,5 187,5 237,5 175,0 200,0 187,5 187,5 162,5 212,5 175,0 212,5 162,5 162,5 186,5
54
Tabel Diameter Tubulus Seminiferus Kelompok Kontrol Asap Rokok 1 Tubulus 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Ratarata
X
Sok 11 12 12 10 10 10 11 13 11 10 13 11 11 11 13 11 13 11 8 12 11 10 12 14 12
µm 175,0 137,5 125,0 187,5 162,5 125,0 150,0 150,0 162,5 150,0 162,5 187,5 150,0 150,0 137,5 150,0 175,0 150,0 150,0 137,5 162,5 175,0 175,0 125,0 150,0
Mencit 3 X Sok µm 15 187,5 14 175,0 13 162,5 11 137,5 12 150,0 12 150,0 12 150,0 14 175,0 10 125,0 14 175,0 11 137,5 13 162,5 15 187,5 12 150,0 11 137,5 11 137,5 12 150,0 11 137,5 12 150,0 13 162,5 13 162,5 14 175,0 10 125,0 10 125,0 11 137,5
154,5
153,0
2 µm 137,5 150,0 150,0 125,0 125,0 125,0 137,5 162,5 137,5 125,0 162,5 137,5 137,5 137,5 162,5 137,5 162,5 137,5 100,0 150,0 137,5 125,0 150,0 175,0 150,0 141,5
X
Sok 14 11 10 15 13 10 12 12 13 12 13 15 12 12 11 12 14 12 12 11 13 14 14 10 12
4 X
Sok 15 10 12 12 10 13 13 13 12 12 12 13 12 12 13 13 13 14 10 11 13 13 13 12 10
5 µm 187,5 125,0 150,0 150,0 125,0 162,5 162,5 162,5 150,0 150,0 150,0 162,5 150,0 150,0 162,5 162,5 162,5 175,0 125,0 137,5 162,5 162,5 162,5 150,0 125,0 153,0
X
Sok 12 11 10 11 12 14 12 13 12 16 11 11 15 13 11 10 12 11 16 10 14 12 11 10 13
µm 150,0 137,5 125,0 137,5 150,0 175,0 150,0 162,5 150,0 200,0 137,5 137,5 187,5 162,5 137,5 125,0 150,0 137,5 200,0 125,0 175,0 150,0 137,5 125,0 162,5 151,5
55
Tabel Diameter Tubulus Seminiferus Kelompok PT Dosis I 1 Tubulus 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Ratarata
X
Sok 13 13 13 10 15 11 13 14 10 14 14 14 15 12 10 11 11 11 13 12 10 12 10 16 8
µm 125,0 175,0 175,0 162,5 150,0 175,0 187,5 112,5 162,5 137,5 137,5 175,0 150,0 150,0 162,5 125,0 100,0 162,5 100,0 150,0 137,5 150,0 175,0 137,5 175,0
Mencit 3 X Sok µm 14 175,0 11 137,5 13 162,5 11 137,5 13 162,5 15 187,5 13 162,5 12 150,0 13 162,5 14 175,0 13 162,5 13 162,5 10 125,0 11 137,5 11 137,5 9 112,5 10 125,0 10 125,0 11 137,5 9 112,5 14 175,0 14 175,0 13 162,5 12 150,0 13 162,5
150,0
151,0
2 µm 162,5 162,5 162,5 125,0 187,5 137,5 162,5 175,0 125,0 175,0 175,0 175,0 187,5 150,0 125,0 137,5 137,5 137,5 162,5 150,0 125,0 150,0 125,0 200,0 100,0 152,5
X
Sok 10 14 14 13 12 14 15 9 13 11 11 14 12 12 13 10 8 13 8 12 11 12 14 11 14
4 X
Sok 11 11 12 13 11 10 13 12 11 12 10 10 15 12 15 12 12 13 13 13 12 12 14 14 13
5 µm 137,5 137,5 150,0 162,5 137,5 125,0 162,5 150,0 137,5 150,0 125,0 125,0 187,5 150,0 187,5 150,0 150,0 162,5 162,5 162,5 150,0 150,0 175,0 175,0 162,5 153,0
X
Sok 14 12 12 13 12 11 16 12 13 12 12 12 12 12 13 13 10 12 10 12 12 13 14 12 15
µm 175,0 150,0 150,0 162,5 150,0 137,5 200,0 150,0 162,5 150,0 150,0 150,0 150,0 150,0 162,5 162,5 125,0 150,0 125,0 150,0 150,0 162,5 175,0 150,0 187,5 155,5
56
Tabel Diameter Tubulus Seminiferus Kelompok PT Dosis II 1 Tubulus 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Ratarata
X
Sok 14 15 16 14 16 18 14 14 13 13 15 17 16 16 15 14 11 14 15 11 19 14 14 18 12
µm 187,5 237,5 200,0 187,5 175,0 187,5 175,0 200,0 225,0 150,0 187,5 212,5 187,5 175,0 150,0 187,5 162,5 187,5 187,5 162,5 187,5 162,5 175,0 150,0 212,5
Mencit 3 X Sok µm 12 150,0 13 162,5 13 162,5 13 162,5 14 175,0 13 162,5 13 162,5 10 125,0 13 162,5 13 162,5 13 162,5 12 150,0 13 162,5 14 175,0 12 150,0 13 162,5 15 187,5 15 187,5 14 175,0 14 175,0 12 150,0 13 162,5 12 150,0 13 162,5 13 162,5
184,5
162,5
2 µm 175,0 187,5 200,0 175,0 200,0 225,0 175,0 175,0 162,5 162,5 187,5 212,5 200,0 200,0 187,5 175,0 137,5 175,0 187,5 137,5 237,5 175,0 175,0 225,0 150,0 184,0
X
Sok 15 19 16 15 14 15 14 16 18 12 15 17 15 14 12 15 13 15 15 13 15 13 14 12 17
4 X
Sok 14 14 13 14 17 14 14 13 13 13 13 16 14 16 17 17 14 16 17 14 15 17 13 16 16
5 µm 175,0 175,0 162,5 175,0 212,5 175,0 175,0 162,5 162,5 162,5 162,5 200,0 175,0 200,0 212,5 212,5 175,0 200,0 212,5 175,0 187,5 212,5 162,5 200,0 200,0 185,0
X
Sok 11 13 12 12 13 13 13 14 18 12 15 13 12 14 13 11 13 11 12 15 13 14 12 13 12
µm 137,5 162,5 150,0 150,0 162,5 162,5 162,5 175,0 225,0 150,0 187,5 162,5 150,0 175,0 162,5 137,5 162,5 137,5 150,0 187,5 162,5 175,0 150,0 162,5 150,0 162,0
57
Tabel Diameter Tubulus Seminiferus Kelompok PT Dosis III 1 Tubulus 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Ratarata
X
Sok 12 12 12 12 15 16 15 13 14 14 17 15 16 14 20 13 12 14 13 13 13 12 11 14 11
µm 175,0 175,0 162,5 125,0 175,0 162,5 175,0 187,5 150,0 162,5 162,5 200,0 200,0 187,5 175,0 187,5 162,5 137,5 175,0 175,0 175,0 150,0 150,0 162,5 150,0
Mencit 3 X Sok µm 13 162,5 13 162,5 14 175,0 14 175,0 13 162,5 15 187,5 14 175,0 15 187,5 17 212,5 14 175,0 14 175,0 14 175,0 12 150,0 15 187,5 14 175,0 16 200,0 15 187,5 12 150,0 12 150,0 14 175,0 13 162,5 14 175,0 15 187,5 13 162,5 14 175,0
168,0
174,5
2 µm 150,0 150,0 150,0 150,0 187,5 200,0 187,5 162,5 175,0 175,0 212,5 187,5 200,0 175,0 250,0 162,5 150,0 175,0 162,5 162,5 162,5 150,0 137,5 175,0 137,5 171,5
X
Sok 14 14 13 10 14 13 14 15 12 13 13 16 16 15 14 15 13 11 14 14 14 12 12 13 12
Keterangan : X
Sok : jumlah kotak pada skala grating
4 X
Sok 13 13 14 15 15 15 13 13 15 15 14 12 14 13 14 12 14 13 13 14 13 14 15 13 12
5 µm 162,5 162,5 175,0 187,5 187,5 187,5 162,5 162,5 187,5 187,5 175,0 150,0 175,0 162,5 175,0 150,0 175,0 162,5 162,5 175,0 162,5 175,0 187,5 162,5 150,0 170,5
X
Sok 14 14 14 14 15 13 14 15 14 13 16 15 14 14 12 14 15 15 13 15 16 18 15 14 15
µm 175,0 175,0 175,0 175,0 187,5 162,5 175,0 187,5 175,0 162,5 200,0 187,5 175,0 175,0 150,0 175,0 187,5 187,5 162,5 187,5 200,0 225,0 187,5 175,0 187,5 180,5
LAMPIRAN 4 ANALISIS DATA
Oneway
Descriptives Diameter Tubulus Seminiferus 95% Confidence Interval for Mean N Mean Kontrol akuades
Std. Deviation
Std. Error
Lower Bound
Upper Bound
Minimum Maximum
5 173.300
20.0331
8.9591
148.426
198.174
147.0
190.5
Kontrol asap rokok 5 150.700
5.2512
2.3484
144.180
157.220
141.5
154.5
PT dosis I
5 152.400
2.1036
.9407
149.788
155.012
150.0
155.5
PT dosis II
5 175.600
12.1932
5.4530
160.460
190.740
162.0
185.0
PT dosis III
5 173.000
4.7958
2.1448
167.045
178.955
168.0
180.5
Total
2
15.0866
3.0173
158.773
171.227
141.5
190.5
5
165.000
Test of Homogeneity of Variances Diameter Tubulus Seminiferus Levene Statistic 19.407
df1
df2 4
Sig. 20
.000
ANOVA Diameter Tubulus Seminiferus Sum of Squares
df
Mean Square
Between Groups
3042.500
4
760.625
Within Groups
2420.000
20
121.000
Total
5462.500
24
58
F
Sig. 6.286
.002
59
Post Hoc Tests
Multiple Comparisons Diameter Tubulus Seminiferus Tukey HSD (I) kelompok perlakuan kontrol akuades
(J) kelompok perlakuan
PT dosis III
Sig.
Lower Bound
Upper Bound
22.6000
6.9570
.029
1.782
43.418
PT dosis I
20.9000*
6.9570
.049
.082
41.718
PT dosis II
-2.3000
6.9570
.997
-23.118
18.518
.3000
6.9570
1.000
-20.518
21.118
-22.6000*
6.9570
.029
-43.418
-1.782
-1.7000
6.9570
.999
-22.518
19.118
PT dosis II
-24.9000*
6.9570
.014
-45.718
-4.082
PT dosis III
-22.3000*
6.9570
.032
-43.118
-1.482
kontrol akuades
-20.9000*
6.9570
.049
-41.718
-.082
1.7000
6.9570
.999
-19.118
22.518
PT dosis II
-23.2000*
6.9570
.024
-44.018
-2.382
PT dosis III
-20.6000
6.9570
.053
-41.418
.218
2.3000
6.9570
.997
-18.518
23.118
kontrol asap rokok
24.9000*
6.9570
.014
4.082
45.718
PT dosis I
23.2000*
6.9570
.024
2.382
44.018
PT dosis III
2.6000
6.9570
.996
-18.218
23.418
kontrol akuades
-.3000
6.9570
1.000
-21.118
20.518
22.3000*
6.9570
.032
1.482
43.118
PT dosis I
20.6000
6.9570
.053
-.218
41.418
PT dosis II
-2.6000
6.9570
.996
-23.418
18.218
kontrol akuades
kontrol asap rokok
PT dosis II
Std. Error
kontrol asap rokok
PT dosis I
PT dosis I
(I-J) *
PT dosis III kontrol asap rokok
95% Confidence Interval
Mean Difference
kontrol akuades
kontrol asap rokok
*. The mean difference is significant at the 0.05 level.
60
Homogeneous Subsets
Diameter Tubulus Seminiferus Tukey HSD Subset for alpha = 0.05 kelompok perlakuan
N
1
2
3
kontrol asap rokok
5
150.700
PT dosis I
5
152.400
PT dosis III
5
kontrol akuades
5
173.300
PT dosis II
5
175.600
Sig.
152.400 173.000
.999
Means for groups in homogeneous subsets are displayed.
.053
173.000
.996
LAMPIRAN 5 FOTO-FOTO PENELITIAN
1. Pasta Tomat “X”
4. Pemberian Asap Rokok
2. Rokok Berfilter “Y” 4. Dislokasi servikal
3. Pemberian Aquades dan Pasta
5. Pengambilan Data
Tomat
61
62
6. Preparat Perbesaran 10 X Kelompok Kontrol Akuades
Kelompok Pasta Tomat Dosis II
Kelompok Kontrol Asap Rokok
Kelompok Pasta Tomat Dosis III
Kelompok Pasta Tomat Dosis I
63
7. Preparat Perbesaran 40 X Kelompok Kontrol Akuades
Kelompok Pasta Tomat Dosis II
Kelompok Kontrol Asap Rokok
Kelompok Pasta Tomat Dosis III
Kelompok Pasta Tomat Dosis I
LAMPIRAN 6 ETIK PENELITIAN
64
RIWAYAT HIDUP
Nama
: Vikie Nouvrisia Anandaputri
NRP
: 0610045
Tempat dan Tanggal Lahir
: Bogor, 28 November 1987
Alamat
: Jl. Kota Baru no. 20A Bogor 16710
Riwayat Pendidikan
:
TK Mexindo Bogor, 1994 SDN Polisi 4 Bogor, 2000 SMP Negeri 1 Bogor, 2003 SMA Regina Pacis Bogor , 2006 Mahasiswa Fakultas Kedokteran Universitas Kristen Maranatha Bandung angkatan 2006
65
