Lampiran 1. Data Penentuan ph. Jenis Formula I II III IV Rata-Rata 4,8 4,8 4,9 4,83

Lampiran 1 Data Penentuan pH

Tabel 7. Data Hasil Penentuan pH Sediaan Jenis Formula PH I II III 1 5,2 4,8 4,4 2 5,5 4,8 4,5 3 5,4 4,9 4,4 Rata-Rata 5,37 4,83 4,43

IV 4,1 4,0 4,0 4,03

Universitas Sumatera Utara

Lampiran 2 Data Penentuan Viskositas

a. Data Penentuan Viskositas Sediaan Tabel 8. Data Hasil Penentuan Viskositas sediaan Jenis Formula Pengulangan I II III 1 170,000 P 147,357 P 142,202 P 2 170,803 P 148,243 P 142,570 P 3 170,630 P 149,753 P 143,074 P Rata-Rata 170,477 P 148,455 P 142,615 P

IV 137,536 P 137,148 P 137,594 P 137,426 P

b. Perhitungan Viskositas Sediaan Data Viskositas untuk Formula I, Formula II, Formula III, dan Formula IV Jenis Formula Spesifikasi I II III IV Berat (gram) 8,865 8,211 7,426 7,2742 Volume(ml) 10 10 10 10 8862 7650 7345 7097 Waktu 8905 7696 7364 7077 (sekon) 8896 7701 7390 7100

k

= 2r2 . g / 9 l

Rumus :

η = k (ρk-ρF1) t

= 2(0,0075)2 . 10 / 9 (0,10) = 1,25 x 10-3

Keterangan : η

: Viskositas (Poise)

k

: Konstanta viskosimeter

ρk

: Berat jenis bola (gr/ml) = 16,321 gr/ml

ρF

: Berat jenis Sediaan Krim (gr/ml)

t

: waktu (sekon)

r

: Jari-jari bola (m) = 0,0075 m


l

: Panjang antara 2 tanda (m) = 0,10 m

g

: Percepatan grafitasi bumi (10 m/s2) = 10 m/s2

FORMULA I ρF1

= gram/ml = 8,865 / 10 = 0,8865 gr/ml

η1

= 1,25 . 10-3 (16,231-0,8865) 8862 = 170,00 P

η2

= 1,25 . 10-3 (16,231-0,8865) 8905 = 170,803 P

η3

= 1,25 . 10-3 (16,231-0,8865) 8896 = 170,630 P

η

= (η1 + η2 + η3) / 3 = (170,00 + 170,803 + 170,630) / 3 = 170,477 P

FORMULA II ρF1

= 8,211 / 10 = 0,8211 gr/ml

η1

= 1,25 . 10-3 (16.231-0,8211) 7650 = 147,357 P

η2

= 1,25 . 10-3 (16,231- 0,8211) 7696 = 148,243 P

η3

= 1,25 . 10-3 (16,231-0,8211) 7701 = 149,753 P


η

= (147,357 + 148,243 + 149,753) / 3 = 148,455 P

FORMULA III ρF1

= 7,426/10

η1

= 1,25 . 10-3 (16,231-0,7426) 7345

= 0,7426 gr/ml

= 142,202 P η2

= 1,25 . 10-3 (16,231-0,7426) 7364 = 142,570 P

η3

= 1,25 . 10-3 (16,231-0,7426) 7390 = 143,074 P

η

= (142,202 + 142,570 + 142,074) / 3 = 142,615 P

FORMULA IV ρF1

= 7,2742 / 10 = 0,72742 gr/ml

η1

= 1,25 . 10-3 (16,231-0,72742) 7097 = 137,536 P

η2

= 1,25 . 10-3 (16.231-0,72742) 7077 = 137,148 P

η3

= 1,25 . 10-3 (16.231-0,72742) 7100 = 137,594 P

η

= (137,536 + 137,148 + 137,594) / 3 = 137,426 P


Lampiran 3 Data Pengamatan Stabilitas Sediaan Tabel 9. Hasil Data Pengamatan Stabilitas Formula I, Formula II, Formula III, dan Formula IV. Pengamatan Sediaan No. Formula 4 Minggu 8 Minggu 12 Minggu x y z X y z x y z 1 I 2 II 3 III 4 IV -

Keterangan : x

: Perubahan warna

y

: Perubahan bau

z

: Pecahnya emulsi

-

: Tidak terjadi


Lampiran 4 Data Pengukuran Serapan Sediaan

Tabel 10. Data Hasil Pengukuran Serapan Formula I λ (nm) Pengulangan 290 300 310 320 330 1 0,5605 0,5538 0,5498 0,4660 0,2913 2 0,5826 0,5758 0,5710 0,4889 0,3126 3 0,5671 0,5640 0,5563 0,4747 0,2992 4 0,5415 0,5380 0,5435 0,4641 0,2947 5 0,5291 0,5255 0,5264 0,4473 0,2810 6 0,5184 0,5160 0,5184 0,4386 0,2697 Jumlah 3,2992 3,2731 3,2654 2,7796 1,7485 Rata-rata 0,5499 0,5455 0,5442 0,4633 0,2914

Tabel 11. Data Hasil Pengukuran Serapan Formula II λ (nm) Pengulangan 290 300 310 320 330 1 0,5845 0,5846 0,5844 0,4984 0,3129 2 0,5970 0,5966 0,5959 0,5089 0,3213 3 0,5780 0,5781 0,5787 0,4934 0,3063 4 0,5696 0,5719 0,5851 0,5002 0,3123 5 0,5864 0,5874 0,5930 0,5071 0,3209 6 0,5776 0,5767 0,5830 0,4966 0,2096 Jumlah 3,4931 3,4953 3,5201 3,0046 1,7833 Rata-rata 0,5822 0,5826 0,5867 0,5008 0,2972

340 0,1437 0,1620 0,1504 0,1500 0,1354 0,1273 0,8688 0,1448

340 0,1521 0,1600 0,1484 0,1517 0,1614 0,1488 0,9224 0,1537

350 0,0058 0,0834 0,0573 0,0608 0,0552 0,0613 0,3238 0,0540

350 0,0654 0,0704 0,0634 0,0651 0,0643 0,0673 0,3959 0,0660


Lampiran 4 (Lanjutan) Tabel 12 Data Hasil Pengukuran Serapan Formula III λ (nm) Pengulangan 290 300 310 320 330 1 0,6432 0,6472 0,6528 0,5542 0,3389 2 0,6583 0,6615 0,6602 0,5626 0,3508 3 0,6447 0,6490 0,6525 0,5553 0,3398 4 0,6589 0,6627 0,6781 0,5823 0,3693 5 0,6576 0,6610 0,6724 0,5737 0,3627 6 0,6520 0,6549 0,6599 0,5662 0,3533 Jumlah 3,9147 3,9363 3,9759 3,3943 2,1148 Rata-rata 0,6525 0,6561 0,6627 0,5657 0,3525

340 0,1566 0,1678 0,1580 0,1862 0,1818 0,1737 1,0241 0,1707

350 0,0702 0,0673 0,0701 0,0922 0,0791 0,0803 0,4592 0,0765

Tabel 13. Data Hasil Pengukuran Serapan Formula IV λ (nm) Pengulangan 290 300 310 320 330 1 0,6797 0,6748 0,6755 0,5784 0,3696 2 0,6680 0,6648 0,6626 0,5669 0,3589 3 0,6753 0,6724 0,6720 0,5759 0,3683 4 0,6981 0,6945 0,7035 0,6102 0,4095 5 0,6769 0,6724 0,6832 0,5909 0,3899 6 0,6646 0,6619 0,6709 0,5778 0,3752 Jumlah 4,0626 4,0408 4,0677 3,5001 2,2714 Rata-rata 0,6771 0,6735 0,6780 0,5834 0,3786

340 0,1879 0,1759 0,1854 0,2306 0,2135 0,1974 1,1907 0,1985

350 0,0845 0,0824 0,0840 0,1174 0,1160 0,1165 0,6008 0,1001


Lampiran 5 Contoh Perhitungan Nilai SPF

Tabel 14. Contoh Data Formula I λ (nm)

A

290

0,5605

300

0,5538

310

0,5498

320

0,4660

330

0,2913

340

0,1437

350

0,0579

Rumus : Area dibawah kurva = luas trapezium = Serapan pada λn + Serapan pada λn+1 2

x (λn+1-λn)

L1 = 0,5605 + 0,5538 x (300 – 290) = 5,5715 2 L2 = 0,5538 + 0,5498 x (310 – 300) = 5,5180 2 L3 = 0,5498 + 0,4660 x (320 – 310) = 5,0790 2


Lampiran 5 (Lanjutan) L4 = 0,4660 + 0,2913 x (330 – 320) = 3,7865 2 L5 = 0,2913 + 0,1437 x (340 – 330) = 2,1750 2 L6 = 0,1437 + 0,0579 x (350 – 340) = 1,008 2 AUC

= L1 + L2 + L3 + L4 + L5 = 5,5715 + 5,5180 + 5,0790 + 3,7865 + 2,1750 + 1,008 = 23,138

Log SPF =

Log SPF

=

AUC λmax - λmin

23,138

x 2

x 2

(350 – 290) = 0,7713

SPF

= Arc. 0,7713 = 5,905


Lampiran 6 Perhitungan Statistik Metode ANOVA

Tabel 15. Rancang Acak Lengkap Ulangan (r)

Perlakuan (t) I

II

III

IV

1

I1

II1

III1

IV1

2

I2

II2

III2

IV2

3

I3

II3

III3

IV3

4

I4

II4

III4

IV4

5

I5

II5

III5

IV5

6

I6

II6

III6

IV6

Jumlah (TA)

ΣI

ΣII

ΣIII

ΣIV

Rataan (yA)

yI

yII

yIII

yIV


Lampiran 6 (Lanjutan)

Tabel 16. ANOVA Sumber Variasi

Df

SS

MS

Fhitung

Perlakuan

t–1

SST

MST

MST

Error Percobaan

n–t

SSE

MSE

MSE

Total

SSY

Ftabel F0,05;(t-1);(n-t)

Keterangan : SST

= Treatment of Squares atau Jumlah Kuadrat Perlakuan

SSE

= Error Sum of Squares atau Jumlah Kuadrat Error

SSY

= Jumlah Kuadrat Total

df

= Degree of Fredom atau Tingkat Kebebasan

t

= Perlakuan

n

= Jumlah Subjek dalam percobaan

MS

= Mean Squares

MST

= SST / t-1

MSE

= SSE / n-t


Lampiran 7 Data Perhitungan ANOVA

Table 17. Nilai SPF untuk Formula I, Formula II, Formula III, dan Formula IV REPLIKASI

I 5,9056 6,522 6,1038 5,8052 5,4439 5,2475 35,028 5,838

1 2 3 4 5 6 Jumlah Rata-Rata t

SST =

∑

JENIS FORMULA II III 6,5960 7,9824 6,8992 8,3541 6,4348 8,0167 6,4991 8,9409 6,7876 8,7093 6,4921 8,4085 39,7088 50,4115 6,6181 8,4019

IV 9,0386 8,6053 8,9375 10,4396 9,5966 9,0764 55,694 9,2823

(Y2i. / r) - (Y2.. / tr)

i =1

= [(35,0,28)2 + (39,7088)2 + (50,4115)2 + (55,694)2] / 6 - (5,9056 + … + 9,0764)2 / 4.6 = 8446,890549 / 6 - 32704,08214 / 24 = 1407,815092 - 1362,670089 = 45,145003 SSY =

t

r

i =1

j =1

∑ ∑

(Y2ij) - (Y2.. / tr)

= [(5,9056)2 + (6,522)2 + … + 9,0764)2] - 1362,670089 = 1411,870673 – 1362,670089 = 49,20058 SSE = SSY – SST = 49,200584 – 45,145003 = 4,055581



MST = =

SST

t −1

45,145003

4 −1

= 15,04833433 MSE = =

SSE

n−t

4,055581

24 − 4

= 0,20277905 Fhitung

Ftabel

=

MST

=

45,04833433

=

74,2104982

=

F0,05;(4-1);(24-4)

=

F0,05;3;20

=

3,10

MSE 0,20277905

Fhitung > Ftabel maka dilakukan uji HSD (Beda antar rata-rata)


Lampiran 8 Hasil Perhitungan ANOVA Descriptives SPF

N Formula I Formula II Formula III Formula IV Total

6 6 6 6 24

Mean 5,838000 6,618133 8,401983 9,282333 7,535113

Std. Deviation ,4578551 ,1854032 ,3772806 ,6507117 1,4625853

Std. Error ,1869185 ,0756905 ,1540242 ,2656519 ,2985490

95% Confidence Interval for Mean Lower Bound Upper Bound 5,357511 6,318489 6,423565 6,812702 8,006052 8,797915 8,599453 9,965213 6,917517 8,152708

Minimum 5,2475 6,4348 7,9824 8,6053 5,2475

Maximum 6,5220 6,8992 8,9409 10,4396 10,4396

Test of Homogeneity of Variances SPF Levene Statistic 1,838

df2

df1 3

Sig. ,173

20

ANOV A SP F

Between Groups W ithin Groups Total

Sum of Squares 45,152 4,049 49,201

df 3 20 23

Mean S quare 15,051 ,202

F 74,345

Sig. ,000


Lampiran 9 Hasil Uji Beda Rata-Rata

HSD = ± f0,05;t;(n-t) / MSE / r = ± f0,05;4;20x

0,20277 / 6

= ± 2,87 x 0,1838 = ± 0,5275 Daerah Penerimaan -0,5275

+0,5275

Dependent Variable: SPF Tukey HSD

(I) JENIS FORMULA Formula I

Formula II

Formula III

Formula IV

(J) JENIS FORMULA Formula II

Mean Difference (I-J) -,7801333*

Std. Error ,2597710

Sig. ,033

Lower Bound -1,507216

Upper Bound -,053051

Formula III

-2,5639833*

,2597710

,000

-3,291066

-1,836901

Formula IV

-3,4443333*

,2597710

,000

-4,171416

-2,717251

,7801333*

,2597710

,033

,053051

1,507216

Formula III

-1,7838500*

,2597710

,000

-2,510933

-1,056767

Formula IV

Formula I

95% Confidence Interval

-2,6642000*

,2597710

,000

-3,391283

-1,937117

Formula I

2,5639833*

,2597710

,000

1,836901

3,291066

Formula II

1,7838500*

,2597710

,000

1,056767

2,510933

Formula IV

-,8803500*

,2597710

,014

-1,607433

-,153267

Formula I

3,4443333*

,2597710

,000

2,717251

4,171416

Formula II

2,6642000*

,2597710

,000

1,937117

3,391283

Formula III

,8803500*

,2597710

,014

,153267

1,607433

*. The mean difference is significant at the .05 level.



Tukey HSD

a

Subset for alpha = .05 JENIS FORMULA Formula I

N 6

Formula II

6

Formula III

6 6

Formula IV Sig.

1 5,838000

2

3

4

6,618133 8,401983 9,282333 1,000

1,000

1,000

1,000

Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 6,000.


Lampiran 10 Grafik Hasil Pengamatan Serapan Sediaan Tabir Surya

Gambar 2. Grafik Panjang Gelombang vs Serapan pada Pengukuran ke-1











Lampiran 11 Data Sertifikat Zat Aktif

1.1. Sertifikat Zat Aktif Oksibenson


Lampiran 11 (lanjutan) 1.2. Sertifikat Zat Aktif Oktilmetoksisinamat








Lampiran 12 Gambar Sediaan Tabir Surya

Gambar 8. Krim Formula I, II, III,dan IV

Gambar 9. Krim Formula I

Gambar 10. Krim Formula II



Gambar 11. Krim Formula III

Gambar 12. Krim Formula IV

Gambar 13. Formula Terlihat dari Sisi Atas


Lampiran 13 Gambar Alat Yang Digunakan

1

1

2

Gambar 14. Alat Spektrofotometer UV-Vis Keterangan :

1. Monitor 2. Printer



1 2

4 5 6 3

Gambar 15. Alat Viskosimeter Bola Jatuh menurut Hoeppler Keterangan :

1. Tudung Penutup 2. Tanda atas 3. Bola 4. Pipa Jatuh 5. Tanda bawah 6. Tudung Penutup


Lampiran 14 Tabel Distribusi Fisher (F)


Lampiran 1. Data Penentuan ph. Jenis Formula I II III IV Rata-Rata 4,8 4,8 4,9 4,83

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