LAMPIRAN 1 Gambar / foto sel mitosis pada jaringan karsinoma epidermoid (anak panah merah dengan perbesaran 400x)
Kelompok kontrol
Kelompok P2
Kelompok P1
Kelompok P3
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LAMPIRAN 2 Analisa Statistik Perubahan Diameter Tumor Case Summaries Selisih diameter tumor Kelompok K P1 P2 P3 Total
N 3 3 3 3 12
Mean 1.1000 .4333 .4000 -.2667 .4167
Std. Deviation .26458 .25166 .10000 .20817 .53739
Median 1.0000 .4000 .4000 -.2000 .4000
Minimum .90 .20 .30 -.50 -.50
Maximum 1.40 .70 .50 -.10 1.40
Case Summaries
Kelompok K
P1
P2
P3
Total
N Mean Std. Deviation Median Minimum Maximum N Mean Std. Deviation Median Minimum Maximum N Mean Std. Deviation Median Minimum Maximum N Mean Std. Deviation Median Minimum Maximum N Mean Std. Deviation Median Minimum Maximum
Diameter tumor pre test 3 1.3000 1.47986 .6000 .30 3.00 3 .5667 .55076 .3000 .20 1.20 3 1.1000 1.47986 .4000 .10 2.80 3 .9667 1.15902 .4000 .20 2.30 12 .9833 1.08362 .4000 .10 3.00
Diameter tumor post test 3 2.4000 1.34536 2.0000 1.30 3.90 3 1.0000 .78102 .6000 .50 1.90 3 1.5000 1.57162 .8000 .40 3.30 3 .7000 .95394 .2000 .10 1.80 12 1.4000 1.22771 1.0500 .10 3.90
77
Tests of Normality a
Selisih diameter tumor
Kelompok K P1 P2 P3
Kolmogorov-Smirnov Statistic df Sig. .314 3 .219 3 .175 3 .292 3
. . . .
Statistic .893 .987 1.000 .923
Shapiro-Wilk df 3 3 3 3
Sig. .363 .780 1.000 .463
a. Lilliefors Significance Correction
Test of Homogeneity of Variances Selisih diameter tumor Levene Statistic 1.129
df1
df2 3
8
Sig. .394
NPar Tests Kruskal-Wallis Test
78
Mann Whitney Test
Test Statisticsb
Mann-Whitney U
Tumor diameter diference .000
Wilcoxon W
6.000
Z
-1.964
Asymp. Sig. (2-tailed)
.049
Exact Sig. [2*(1-tailed Sig.)]
.100
a
a. Not corrected for ties. b. Grouping Variable: Group
Test Statisticsb
Mann-Whitney U
Tumor diameter diference .000
Wilcoxon W Z
6.000 -1.964
Asymp. Sig. (2-tailed)
.049
Exact Sig. [2*(1-tailed Sig.)]
.100
a
a. Not corrected for ties. b. Grouping Variable: Group
79
Test Statisticsb
Mann-Whitney U
Tumor diameter diference .000
Wilcoxon W Z
6.000 -1.964
Asymp. Sig. (2-tailed)
.049
Exact Sig. [2*(1-tailed Sig.)]
.100
a
a. Not corrected for ties. b. Grouping Variable: Group
80
Test Statisticsb
Mann-Whitney U
Tumor diameter diference .000
Wilcoxon W
6.000
Z
-1.964
Asymp. Sig. (2-tailed)
.049
Exact Sig. [2*(1-tailed Sig.)]
.100
a
a. Not corrected for ties. b. Grouping Variable: Group
Test Statisticsb
Mann-Whitney U
Tumor diameter diference .000
Wilcoxon W Z
6.000 -1.964
Asymp. Sig. (2-tailed)
.049
Exact Sig. [2*(1-tailed Sig.)]
.100
a
a. Not corrected for ties. b. Grouping Variable: Group
81
LAMPIRAN 3 Analisa Statistik Indek Mitosis
Case Summaries Index mitosis Kelompok K P1 P2 P3 Total
N
Mean 3.2000 1.1333 1.0667 .6667 1.5167
3 3 3 3 12
Std. Deviation .34641 .11547 .11547 .11547 1.04606
Median 3.4000 1.2000 1.0000 .6000 1.1000
Minimum 2.80 1.00 1.00 .60 .60
Maximum 3.40 1.20 1.20 .80 3.40
Tests of Normality a
Index mitosis
Kelompok K P1 P2 P3
Kolmogorov-Smirnov Statistic df Sig. .385 3 .385 3 .385 3 .385 3
. . . .
Statistic .750 .750 .750 .750
Shapiro-Wilk df 3 3 3 3
Sig. .000 .000 .000 .000
a. Lilliefors Significance Correction
Test of Homogeneity of Variances Index mitosis Levene Statistic 5.333
df1
df2 3
Sig. .026
8
NPar Tests
Kruskal-Wallis Test Ranks Index mitosis
Kelompok K P1 P2 P3 Total
N 3 3 3 3 12
Mean Rank 11.00 7.00 6.00 2.00
82
Test Statisticsa,b Chi-Square df Asymp. Sig.
Index mitosis 9.804 3 .020
a. Kruskal Wallis Test b. Grouping Variable: Kelompok
NPar Tests
Mann-Whitney Test Ranks Index mitosis
Kelompok K P1 Total
N 3 3 6
Mean Rank 5.00 2.00
Sum of Ranks 15.00 6.00
Mean Rank 5.00 2.00
Sum of Ranks 15.00 6.00
NPar Tests
Mann-Whitney Test Ranks Index mitosis
Kelompok K P2 Total
N 3 3 6
Test Statisticsb Mann-Whitney U Wilcoxon W Z Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
Index mitosis .000 6.000 -2.023 .043 .100
a
a. Not corrected for ties. b. Grouping Variable: Kelompok
83
NPar Tests
Mann-Whitney Test Ranks Index mitosis
Kelompok K P3 Total
N 3 3 6
Mean Rank 5.00 2.00
Sum of Ranks 15.00 6.00
Mean Rank 4.00 3.00
Sum of Ranks 12.00 9.00
Test Statisticsb Mann-Whitney U Wilcoxon W Z Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
Index mitosis .000 6.000 -2.023 .043 .100
a
a. Not corrected for ties. b. Grouping Variable: Kelompok
NPar Tests
Mann-Whitney Test Ranks Index mitosis
Kelompok P1 P2 Total
N 3 3 6
84
Test Statisticsb Mann-Whitney U Wilcoxon W Z Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
Index mitosis 3.000 9.000 -.745 .456 .700
a
a. Not corrected for ties. b. Grouping Variable: Kelompok
NPar Tests
Mann-Whitney Test
Ranks Index mitosis
Kelompok P1 P3 Total
N 3 3 6
Mean Rank 5.00 2.00
Sum of Ranks 15.00 6.00
Test Statisticsb Mann-Whitney U Wilcoxon W Z Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
Index mitosis .000 6.000 -2.023 .043 .100
a
a. Not corrected for ties. b. Grouping Variable: Kelompok
85
NPar Tests
Mann-Whitney Test
Ranks Index mitosis
Kelompok P2 P3 Total
N 3 3 6
Mean Rank 5.00 2.00
Sum of Ranks 15.00 6.00
Test Statisticsb Mann-Whitney U Wilcoxon W Z Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
Index mitosis .000 6.000 -2.023 .043 .100
a
a. Not corrected for ties. b. Grouping Variable: Kelompok
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LAMPIRAN 4 Uji Korelasi Selisih Diameter Tumor dan Indek Mitosis
Explore
Case Summaries
N Mean Std. Deviation Median Minimum Maximum
Selisih diameter tumor 12 .4167 .53739 .4000 -.50 1.40
Index mitosis 12 1.5167 1.04606 1.1000 .60 3.40
Tests of Normality a
Selisih diameter tumor Index mitosis
Kolmogorov-Smirnov Statistic df Sig. .105 12 .200* .369 12 .000
Statistic .988 .742
Shapiro-Wilk df 12 12
Sig. .999 .002
*. This is a lower bound of the true significance. a. Lilliefors Significance Correction
Nonparametric Correlations
Correlations
Spearman's rho
Selisih diameter tumor
Index mitosis
Correlation Coefficient Sig. (2-tailed) N Correlation Coefficient Sig. (2-tailed) N
Selisih diameter tumor Index mitosis 1.000 .813** . .001 12 12 .813** 1.000 .001 . 12 12
**. Correlation is significant at the 0.01 level (2-tailed).
87
LAMPIRAN 5 Patologi Anatomi Sampel, Ukuran Diameter Tumor Pre dan Post Perlakuan, Indek Mitosis Post Perlakuan SCORING INDEX MITOSIS DALAM 5 LAPANG PANDANG BESAR KELOMPOK Kontrol
I
II
III
IV
V
Mean
A
4
3
3
5
2
3.4
B C mean P1
2 4
3 3
4 2
3 3
2 5
2.8 3.4 3.2
A
1
2
1
1
1
1.2
B
0
1
2
1
1
1
C Mean P2
3
1
1
1
0
1.2 3.4
A
1
1
1
0
2
1
B
2
2
0
1
1
1.2
C
1
0
2
2
0
1
mean P3
SQUAMOUS CELL CA WELL DIFFERENTIATED SQUAMOUS CELL CA Insitu SQUAMOUS CELL CA Insitu
KERATOACANTOMA DENGAN SQUAMOUS CELL CA MIKROINVASIF PAPILOMA CELL SQUAMOUS DENGAN FOKUS CA SQUAMOUS CELL CA Insitu
SQUAMOUS CELL CA WELL DIFF DENGAN NEKROSIS LUAS SQUAMOUS CELL CA WELL DIFFERENTIATED SQUAMOUS CELL CA WELL DIFFERENTIATED
1.06
A
0
1
1
2
0
0.8
B
1
0
0
1
1
0.6
C
1
1
0
1
0
0.6
mean
HASIL PATOLOGI ANATOMI
SQUAMOUS CELL CA WELL DIFFERENTIATED SQUAMOUS CELL CA Insitu SQUAMOUS CELL CA INSITU + MIKROINVASIF
0.6
88
Data Ukuran Diameter Tumor Pre dan Post Perlakuan Kelompok Kontrol A B C Mean P1 A B C Mean P2 A B C Mean P3 A B C Mean
Diameter tumor pre test (cm)
Diameter tumor post test (cm)
3 0,6 0,3
3,9 2 1,3
0,9 1,4 1 1,1
1,2 0,2 0,3
1,9 0,6 0,5
0,7 0,4 0,2 0,43
2,8 0,1 0,4
3,3 0,4 0,8
0,5 0,3 0,4 0,4
2,3 0,4 0,2
1,8 0,2 0,1
-0,5 -0,2 -0,1 -0,26
Selisih diameter tumor (cm)
Keterangan : K
: Kontrol
P1
: Phaleria macrocarpa 0,0715 mg (0,36 ml/hari).
P2
: sitostatika Paclitaxel 175 mg/m2 dan cisplatin 50 mg/m2
P3
: sitostatika Paclitaxel 175 mg/m2 dan cisplatin 50 mg/m2 dan dikombinasikan dengan Phaleria macrocarpa 0,0715 mg (0,36 ml/hari)
A
: tanda asam pikrat pada mencit di kepala
B
: tanda asam pikrat pada mencit di kaki depan
C
: tanda asam pikrat pada mencit di ekor
89
LAMPIRAN 6 Foto Mencit yang Diinduksi Tumor, Perlakuan, dan Pengukuran Tumor
Gambar. Mencit yang diinduksi karsinoma epidermoid
Gambar. Pemberian Phaleria macrocarpa via sonde
Gambar. Penyuntikan sitostatika Gambar. Pengukuran diameter tumor menggunakan caliper
90
LAMPIRAN 7 Foto Pemeliharaan Mencit
91
LAMPIRAN 8 Foto Pengolahan Ekstrak Phaleria macrocarpa
92
LAMPIRAN 9
93
LAMPIRAN 10
94
