Lampiran 1. Analisa Indeks Aterogenik Descriptives
Std. Deviation Std. Error Mean ,58924 ,34020 1,2400 ,06839 1,2267 ,11846 ,62781 1,08740 1.2067 ,62139 ,20713 1,2244 ,12503 ,07219 ,4533 ,65501 ,37817 1,6167 ,4833 ,30665 ,17704 ,22718 ,8511 ,68155 ,I 1780 ,20404 ,3467 1,89739 1,09546 2,5600 1,08752 ,62788 1.8300 1.46973 ,48991 1,5789 ,43062 ,24862 ,4167 1.20952 ,69832 2.9867 ,76632 1.32730 1,7933 1,44674 ,48225 1,7322
N IA-B1
IA-B2
IA-B3
IA-B4
K.Negatif K.positif Statin Total K.Negatif K.positif Statin Total K.Negatif K.positif Statin Total K.Negatif K.positif Statin Total
3 3 3 9 3 3 3 9 3 3 3 9 3 3 3 9
Test of Homogeneity of Variances
IA-B1 [A-62 IA-B3 IA-!34
Levene Statistic 5,242 2,488 4,658 2,734
df2
dfl 2 2 2 2
6 6 6 6
Sig. ,048 ,163 ,060 ,143
ANOVA
95% Confidence Interval for Mean Lower Bound Upper Bound Minimum Maximum -237 2,7037 1,60 56 1,5209 ,9324 1,09 1,30 3,9079 -1.4946 2.43 ,35 1.7021 ,7468 2.43 .35 ,7639 ,1427 ,33 ,58 2,22 3,2438 -,0105 ,92 1,2451 -,2784 -16 ,77 1,3750 ,3272 2.22 ,I6 ,8535 -,I602 ,I7 -57 7.2734 -2,1534 1.12 4-71 4,5315 -.a715 2,81 -66 4,71 2.7086 ,4492 ,I7 1.4864 -6531 ,oo -86 5.9913 -,0179 2,08 4,36 5,0905 -1,5039 2,76 28 2,8443 ,6202 4,36 ,oo
?ost Hoc Tests Indeks atherogenik
Multiple Comparisons
The mean difference is significant at the .05 level.
Homogeneous Subsets Inileks Atherogenik IA-62 Subset for alpha = .05 Periakuan 1 N 2 Duncans K.Negatif 3 ,4533 Statin 3 ,4833 K.positif 3 1.6167 Sig. ,934 1,000 Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Sue = 3,000.
= .05 Perlakuan Duncana Statin K.positii K.Negatif
N
-
1 '1 / 3
1 1.2067 1,2267 1.2400
Sig. ,958 Means for groups in homogeneous subsets are displayed. a. Uses Hamlonic Mean'SampleSue = 3.000.
Subset for alpha
= .05 1 ,3467 1,8300 2,5600 ,085
N
Perlakuan .Duncana K.Negatif Statin K.positif Sig.
[A-w
3 3 3
Subset for alpha = .05 Perlakuan Duncana K.Negatif Statin K.wsitif Sig.
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
N
3 3 3
1 ,4167 1,7933 ,165
2 1.7933 2,9867 ,219
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000
Lampiran 2. Analisa Kolesterol Total Descriptives
95% Confidence Interval for N
81
82
83
84
K-negatif Kgositif Statin Total K-negatif Kpsitif Statin Total K-negatif Kgositif Statin Total K-negatif Kgositi Statin Total
Mean
3 3 3 9 3 3 3 9 3 3 3 9 3 3 3 9
53.1667 49,6333 77,5000 60,1000 43,2600 163.6170 183,3343 130.0704 57,7233 259.3487 2i6,2593 177,7771 68,4210 435.9650 127,1930 210.5263
Mean Std. Deviation Std. Error Lower Bound Upper Bound Minimum Maximum
23,11068 18,79051 23,63810 23,11125 19,31928 96.94089 154.35672 112,74359 47.18961 112,65489 196,70616 147,84524 63,81222 236,26145 81,59314 214,17887
13,34295 10.84871 13,64747 7.70375 11,15399 55,96885 89,11790 37,58120 27,24493 65,04133 113,56836 49.28175 36.84200 136,40561 47,10782 71,39296
Test o f Homogeneity o f Variances Levene Statistic
B1 82 83
84
,210 3,078 4,476 5,767
dfl
df2
2 2 2 2
Sig.
6 6 6 6
,816 ,120 ,065 ,040
-4.2434 2,9551 18,7797 42.3351 -4,7318 -77,1975 -200.1090 43,4080 -59,5021 -20,5016 -272,3859 64.1332 -90,0973 -150.9410 -75,4956 45.8939
110.5768 96,3116 136.2203 77,8649 91,2518 404.4315 566.7777 216,7328 174.9488 539,1989 704,9045 291,4210 226;9393 1022.8710 329,8816 375.1588
38,40 2830 50,9[1 28,80 24,90 78,05 47.56 24.90 29.27 129,27 78,05 29,27 3158 i63,16 44.74 31,58
79,80 65.30 96,IO 96,lO 63,42 268.90 351,22 351,22 112.20 324,39 441,46 441,46 142,ll 573,68 207.90 573.68
ANOVA
Post Hoe Tests Kolestreol TotaI Multiple Comparisons
I I
Dependent Variabit 61
LSD
82
LSD
Mean I I Difference (I) Periakuan (J) Periakua (I-J) Std. E m r K-negatif Kgositif 1 3,53333 17,92544 1 Statin -24,33333 17.92544 Kgositif K-negatif -3,53333 17.92544 Statin -27,86667 17,92544 Statin K-negafif 24,33333 17,92544 Kgositif 27.86667 17,92544 K-negatif Kgositif .120,35700 86,40572 Statin 140,07433 86.40572 Kgositif K-negatif 1120,35700 186,40572 Statin -19,71733 86,40572 Statin K-negatif 140,07433 86,40572 Kgositii 19,71733 86.40572 K-negatif Kgositif -201.62533 09.14954 Statin -158,53600 09.14954 Kpsitif K-flegatif 201,62533 09,14954 Statin 43.08933 09,14954 Statin K-negatif 158,53600 09.14954 Kgositif -43,08933 09,14954 K-negatif Kgositif -367,54400a 21,60858 Statin -58,77200 21.60858 Kgositif K-negatif 367,54400'21,60858 Statin 308,77200^ 21,60858 Statin K-negaui 68,77200 21.60858 Kgositif -308,77200' 21,60E58
1
83
LSD
84
LSD
*.The mean difference is significant at the .05 level.
I
95% Confidence Interval Sig. Lower Bound lupper Bound ,850 -40,3286 1 47,3953 ,223 -68,1953 19,5286 ,850 -47,3953 40,32$6 ,171 -71,7286 15.9953 ,223 -19,5286 68.1953 ,171 -15,9953 71,7286 ,213 -331.7842 91.0702 ,156 -351.5015 71,3529 ,213 -91.0702 331.7842 ,827 -231,1445 191,7099 ,156 -71,3529 351,5015 ,827 -191,7099 231,1445 ,114 , -468,7046 65,4540 ,197 -425.6153 108.5433 ,114 -65,4540 468.7046 ,707 -223,9900 310,1686 ,197 -108,5433 425,6153 ,707 -310,1686 223,9900 ,023 -665,1095 -69,9785 ,646 -356.3375 238.7935 ,023 69,9785 665,1095 11,2065 605,3375 ,044 ,646 -238,7935 356,3375 -606,3375 -11,2065 ,044
1 1
1 1
Homogeneous Subsets Kolesterol T o t a l
Perlakuan Duncana K-negatif Kgositif Statin Sig.
/ 31163.6;;iI (
I
1
N
3
1
3
1 43.2600
183,3343
Means for groups in homogeneous subsets are displayed a. Uses Harmonic Mean Sample Sue = 3,000. &I
I I
I
Perlakuan Duncana K-negatif
I I I
[ N
3
I
1
Subset for alpha = .05
1 68.4210
1
1
2
1
Kgositif 3 435,9650 ,646 1.000 sig. Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000
I
1
Subset for alpha
= .05 Perlakuan
I
Statin Kgositii Sig.
57,7233 216,2593 259,3487 125
1 1 / 3
3
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Sue = 3.000.
1
Lampiran 3. Analisa Trigliserida Descriptives
Test of Homogeneity of Variances
81 B2 83 B4
Levene Statistic 9,865 8.487 ,571 5.662
dfl
df2 2 2 2 2
6 6 6 6
Sig. ,013 ,018 ,593 ,042
ANOVA
Within Groups Total
53860,612 69430,134
6 8
8976,769
lost H o c Tests T r i g l i e r i d a Multiple Comparisons
Bomogeneous Subsets T r i g l i s e r i d a
83
I
I Subset I I for alpha I
I I Perlakuan Duncana K-negatif Kpsitif Statin
Subset for alpha
= .05
N 3
1 52,5667
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3.000.
Perlakuan Duncana Kgositif K-negatii Statin
I
21,7950 28,2050 28.2050 ,438
Sig. Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
Subset for alpha
Subset for alpha
= .05 N
Perlakuan Duncana Kgositif K-negatii Statin Siq.
3 3 3
N
1 44,8720 69,2310 91,0257 .436
Kgositif Statin Sig.
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
3 3 3
= .05 1 53.7633 65.5913 147,3120 .286
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
Lampiran 4. Analisa HDL Descriptives
04
Statin Total K-negatif Kgositif Statin Total
3 9 3 3 3 9
98.28183 62,72067 22.33841 70,32842 16,21919 48.22758
138,6227 92.6437 53.4050 113.1667 53.W17 73.1911
56.74304 20,90689 12,89709 40,60298 9.38416 16.07586
Test of Homogeneity of Variances
Levene Statistic
B1 82 83 84
4,358 3,764 2,407 2,415
Sig.
df2
dfl
2 2 2 2
6 6 6 6
,068 ,087 ,171 ,170
-107.5229 44,4323 -2.0867 61.5339 12.7110 35.1201
380.7683 140.8550 108.8967 287.8672 93.2924 110,2621
33.89 33.89 31.72 46.37 34.27 31,72
229,74 229.74 76.34 186.56 62.50 186.56
ANOVA
Post Hoc Tests HDL Multiple Comparisons
Homogeneous Subsets HDL 53
81
Subset for alpha
= .05 1 22,0333 24,0667 39.6333 ,172
N
Perlakuan Duncana Kgositif K-negatif Statin Sig.
Subset for alpha
3 3 3
= .05
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
I Perlakuan Duncana K-negatif Kgositif Statin Siq.
1
I
N
I
3 3 3
1
Subset
N
Perlakuan Duncana K-negatif Kgositii Statin Sig.
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
1 64.1130 77,1953 136,6227 ,210
3 3 3
Means for groups in homogeneous subsets are displayed. a, Uses Harmonic Mean Sample Size = 3,000.
I
1 60,7527 61,5143 117,9213 ,197
1 Perlakuan Duncana Statin K-negatii Kgositii Siq.
1
I
1 N
Subset
Statin 84
K-rmgatif
K-vositif
Statin Total
3 9 3 3 3 9
128.8250 120,7298 33.6107 161,9440 82,3729 92.6423
139,06797 80,29093 108.68453 36.22818 20.90852 12,07154 176.02563 101.62844 8,31003 4,79780 104.97695 34.99232
1
1
3
1 53.0017
3 3
53,4050 113.1667 ,154
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
Lampiran 5. Analisa LDL
Total
,
-216.6390 37.1874 -1 8.3290 -275.3279 61.7291 11,9499
474,2890 204,2721 85.5503 599.2159 103.0156 173.3348
33.90 11,51 111.15 58.42 74.74 10.15
288.46 288.46
50.23 365.19 91.23 365.19
>
Test of HornogeneiQ of Variances Levene Statistic
B1 82 83
.64
3.094 2,441 1.087 13,270
dfl
d? f.
2 2 2 2
Sig.
6 6 6 6 ANOVA
,119 ,168 ,395 ,006
?ost Hoe Tests LDL Multiple Comparisons
Homogeneous Subsets LDL
Perlakuan Duncan8 Kgositif K-negatif Statin Sig.
N 3 3 3
Subset for alpha = .05 1 16.2667 18.5867 47,2980 ,123
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
Perlakuan Duncane K-negatif Statin Kgositif Sig.
N 3 3 3
Subset for alpha = .05 1 55,5033 128,8250 177.8610 ,233
Means for groups in homogeneous subsets are displayed a. Uses Harmonic Mean Sample Sue = 3,000.
Subset for alpha
Subset for alpha
= .05 1 14.8850 63,8477 92,9277 ,065
N
Perlakuan Duncana K-negatif Statin Kgositif Sig.
3 3 3
Perlakuan Duncana K-negatif Statin KgosiM Sig.
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3.000.
N 3 3 3
= .05 1 33,6107 82,3723 161,9440 ,189
- Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample She = 3.000.
Lampiran 6. Analia Rataan Berat Badan Descriptives rataan BB
95% Confidence Interval for
- K-negatif
Mean
N
3 3 3 9
Kgositii Statin Total
Std. Deviation
2162.5933 2386,7800 2084.0900 2211.1544
Mean Lower Bound Upper Bound
Std. Error
1983,1379 1829,1944 1458.3201 2042,2752
72.24054 41,70809 224,45839 129.59111 251.90628 145,43816 219,70371 73.23457
Test o f Homogeneity of Variances Levene Statistic
df2
dfl
1,258
2
Sig.
6
,350 ANOVA
rataan BB Sum of Squares Between Groups Wthin Groups Total
148043.7 2381 14,l 386157,7
Mean Square
df
2 6 8
74021.835 39685,679
F 1,865
Sig.
,234
2342.0488 2944,3656 2709.8599 2380,0337
Minimum
2080.85 2157.91 1855,18 1855,18
Maximum
2217.86 260635 2353.97 2606,55
ost Hoc Tests Rataan BB Multiple Comparisons Dependent Variable: rataan BB
I LSD
I
I
I
)
Mean Difference (I-J) (J) Perlakuan Kgositif ( -224.18667 Statin ( 78.50333 K-negatii 224.18667 Statin 302,69000 K-negatif 1 -78,50333 Kgositif -302,69000
(I) Perlakuan K-negatif -
1 1
Kgositif Statin
Std. Error 1162,65645 (162,65645 1162,65645 1162,65645 1162,65645 162,65645
,217
95% Confidence Interval Lower Bound U er Bound -622,1927 173,8193 -319,5027 476,5093 -173,8193 622,1927
:omogeneous Subsets Rataan BB
Subset for alpha Perlakuan Duncana Statin K-negatif Kgositif Siq.
)
N
I
1 3 12084,0900 3 2162,5933 3 2386,7800 ,122
Means for groups in homogeneoussubsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
ampiran 7. Analisa Konsumsi Pakan
rataan konsum pakan
-
Mean 83,0333 62,8500 74,6867 80,1900
N
K negatif Kgositif Statin Total
3 3 3 9
Std. Deviation 7,96406 10,56821 10.64226 9,441 15
Test of Homogeneity of Variances rataan konsum pakan Levene Statistic dfl ,143 2
df2
(
6
(
Sig. ,869
Std. Error 4,59805 6,10156 6,14431 3,14705
95% Confidence Interval for Mean Lower Bound Upper Bound 102,8172 63.2495 109.1029 56,5971 101,1235 46.2498 87,4471 72.9329
Minimum 76.85 72,60 66.12 66,12
Maximum 92,02 93,71 86.60 93,71
-
ANOVA
.
rataan konsum pakan Sum of Squares Between Groups 136,340 Within Groups 576.742 Total 713,083
Mean Square
df
2 6 8
F
68.170 96,124
,ostHoc Tests Konsumsi pakan Multiple Comparisons
iomogeneous Subsets Konsurnsi pakan
Subset for alpha Perlakuan Duncana Statin Kgositif K-negatii Sig.
I
I
N
1
3
1 74.6867
3 3
82,8500 83,0333 .352
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
,709
Sig.
,529
.ampiran 8. Analisa Konversi Pakan Descriptives
I
rasio konvers pakan
t
I
I
I
Std. Deviation
Mean
N K-negatif Kgositif Statin Total
1 ,14731 ,69635 ,49743 ,54035
,4300 1.1200 ,5367 .6956
3 3 3 9
95% Confidence Interval for Mean Lower Bound Upper Bound
Std. Error
,08505 ,40204 ,28719 ,18012
,0641 -,6098 -,6990 ,2802
Test o f Homogeneity of Variances rasio konvers pakan ~evene Statistic dfl
I
I I 4.579 1
2
1
I
df2
6
1
Sig.
1
,062 ANOVA
rasio konvers pakan Sum of Squares Between Groups ,828 Within Groups 1,508 Total 2,336
df
Mean Square
2 6 8
,414 ,251
F 1.647
Post Hoc Tests KOnversi Pakan Multiple Comparisons
Sig.
,269
,7959 2,8498 1,7723 1.1109
I
I Minimum
26 -65 -22 .22
Maximum
32 1.92 1,11 1.92
Homogeneous Subsets Konversi Pakan rasio-konversgakan
I
Perlakuan Duncan8 K-negatif
I
N 3
1
Subset for alpha = .05 1 ,4300
I
,154 Sig. Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
Lampiran 9. Analisa Tebal Plak Atheroma Descriptives tebal olak ~ ~
N K-negatif Kgositif Statin Total
3 3 3 9
Std. Deviation ,35553 ,37170 2.84649 1,88743
Mean ,5318 1,5162 3,2946 1,7809
Std. Error ,20526 ,21460 1,64342 ,62914
95% Confidence Intewal for Mean Lower Bound Upper Bound -,3513 1.4150 2,4395 ,5929 10,3657 -3,7765 3,2317 ,3301
Test of Homogeneity of Variances tebal plak
I ~evene I statistic I 10.306
dfl
1
1
dP2 2 1
6
I
sin.
1
,011
ANOVA tebalglak
Between Groups Within Groups Total
Sum of Squares 11,765 16,734 28,499
df 2 6 8
Mean Square 5,882 2,789
F 2,109
Sig. ,202
Minimum -12 1.09 1,31 ,12
Maximum ,77 1,78 6,56 6,56
'ost Moc Tests T%balPlak Atheroma iviultiple Comparisons
lomogeneous Subsets Tebal Plak Atheroma tebalglak Subset for alpha = .05 Perlakuan
)
Kgositif Statin Sig.
1 '1 1 ,5318 1.5162 3,2946
,098 Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
Lampiran 10. Prosedur Standar Potong Beku
1. .Taringan dibekulcan dengan nitrogen cair. 2. Pastikan Cryostat bekerja pada temperatur -20°C sampai 30°C. Selanjutnya,
tempatkan sedikit OCT pada wadah yang telah berisi jaringan. (Gelembung tidak boleh terdapat pada sediaan) 3. Tempatkan spesimen yang telah dibekukan kebagian tengah cryobar pada cryostat
untuk proses pembekuan. Blok spesimen akan berwama putih solid saat telah membeku. 4. Bila spesilnen telah beku sempuma, tempatkan sedikit cairan OCT pada tengah disk
object untuk merekatkan blok spesimen. Selanjutnya tempatkan kembali pada cryobar, hingga spesimen menempel sempuma.
5. Apabila telah sempuma, tempatkan object disk yang telah ditempeli spesimen pada object disk holder dan dieratkan dengan memutar skmp objec dik holder.
6. Pastikan ada cukup jarak antara blok spesimen dengan pisau nzicrolome dan atur tebal spesimen yang akan dipotong dengan memutar pengatur tebal spesimen pada cryotome.
7. Gerakkan blok spesimen kearah pisau microtolne dengan memutar penggerak blok
pada bagian luar cryostat. Potong dan buang beberapa potongan pertama.
8. Selanjutnya ambil beberapa spesimen yang dibutuhkan dan rekatkan pada gelas objek. 9. Proses selanjutnya adalah melakukan pewamaan dengan Sudan Black dan Kemechtrot.
