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Larnpiran 2. Anal isis ragam pengaruh lokasi kebun di Lampung, Cianjur dan Jember terhadap kejadian penyakit busuk buah pada kakao
No. 1.
Sumber keragaman
DB
F hitung Lampung
Kelompok
4
4,05
Total F table 5% = 4,46; 1% = 8,6S m' Tidak nyata.
F hitung Cianjur 1,23
F hitung Jem ber 0,59
14
Lampiran 3. Analisis ragam pengaruh lokasi kebun di Lampung, Cianjur dan Jember terhadap keparahan penyaki t busuk buah pa& kakao
No.
Sumber keragaman
DB
1.
4
F hitung Lampung 3,39
2. 3.
2
0,29 '"
Kefompok Lokasi kebun Galat Total F table 5% = 4,46; 1 % = 8,65 In' Tidak nyata.
F hitung Cianjur 0,64 0,74
F hitung Jemkr 1,15 0,t9'"
8 14
Lampiran 4. AnaIisis ragam pengaruh 37 jenis isoIat 1'. puimivoru terhadap Iuas bercak yang ditimbulkan pada Suah kakao klon Amelonado No.
lsolat Galat Total Sangat r,yata pa& tingkat 1%.
1. 2.
"'
Sumber keragaman
DB
F hiiung
36 74 110
4,72-'
F tabel
5% I ,62
I% f ,98
Lampiran 5. Analisis ragam pengaruh 37 jenis isolat P. pult?iivora terhadap Iuas kanker batang yang ditirnbulkan pada pohon kakao klon Amelonado
No.
Sumber keragaman
DB
F hitung
4
21,96
F tabel 5%
Kelompok isofat Galat Total '7 Sangat nyata pada tingkat 1%. 1. 2. 3.
36 144 1 84
134
1% 1,83
2,2 1**
Lampiran 6 . Analisis ragam pengaruh 24 perlakuan isolat x klon terhadap penode inkubasi (PI), luas bercak (LB) dan laju infeksi (LI) penyakit busuk buah kakao
No. 1.
Sumber keragaman DB 2 Kelompok 2. Isolat x klon 23 46 3. Galat Total --7 1 F table 5% = 1,77; 1% = 2,24 Sangat nyata pada tingkat I %.
"'
F hitung PI
F hitung LB F hitung LI
8,15**
12,89**
1 1,33**
Lampisan 7. Profi 1 pita DNA hasi I am pl ifikasi dua puI uh ( 1-20) isoIat 1'. pu/n?rvotzl dengan primer OPB 1 1 , tM) {marker.
l,ampiran 8
Profil pita DNA hasil aniplitikasi dua puluh ( 1-20] isalaf 1'. p u l ~ ? l i ~ ~dengan o ~ - u primer OPH 19, (M) marker.
Lampiran 9, h f d pi@ DNA h a d a~1p-i P. pa[mnom dengan pwm* marker*
selektif sqxduh (1-10) isald't pdmcr EAC/MCAT, &O
L a a p h PO. Prom pita DNA b.Q - a m p m i seIeWwpyluh (I1-20) isolsrt P. paImjrcror& derigaa pasangan primer EAW-CAT, (M) mdcEr.
1'2
P.
pi@ DNA WI.t @ @ i & W ~ ~ l = Z @ ) *m ctengrsl .. psztzgm primer 6 . ~ c f ; M r n ~(MI ,
Lampiran 13. Matri k hubungan kesamaan genetik dua puluh isoht P.palmivora basil RAPD.
SS-BZ
JB-6 JT-CP ST-10
ST-B
JT-R JB-T- JB-31
ST-9
---------------------------------------------------------------+---
SS-82
JB-6 JT-CP ST-10 ST-a
JT-R
JB-T-CIO JB-B1-CIO ST-9
ST-6 SU- 4
su-3 JB-BLSZ
JB-BLS4 SS-B1 JB-3AP LP-B
LP-T JT-GC7
JB-RAP
SU-3 J B - B L JB-BL S S - B l J B - W
su-3 SB-BLS2
JB-BLS4 SS-81
JB-BAP LP-B
LP-T JT-GC7
JB-RAP
LP-B
LP-TJT-GC7W-RAP
ST-6
SU-4
Larnpiran 14. Matrik hubungan kesamaangenetik dua puluh isolat P. p~lnzivoruhas11 AFLP.
SS-BZ
JB-6 J T - C P ST-10
ST-8
J T - R JB-T- JS-B1
ST-?
ST-6
SU-4
------------------------------------------------------------------SS-B2 38- 6
-.
JT-CP ST-10 ST-8 Jl- R JB-T-CIO JB-B1-CIO ST-9
ST- 6 SU-4
su- 3 JB-3LS2
I I I 1
I I I
1 I I I I
JB-BLS4
I I
SS-B1
1
JB-BAP
I
LP-B LP-T JT-GC7
I
JB-RAP
I
I 1
SU- 3 J E - B L J B - B L SS-BlJB-BAP
SU-3 JB-BLS2 JB-BLS4 SS-B1
JB-BAP LP-k LP-T
JT-GC7 JB-RAP
1 I I
I I I I I I
LP- B
.- - - - --
LP-TJT-GC? J B - R A P
