UNIVERSITAS AIRLANGGA DIREKTORAT PENDIDIKAN Tim Pengembangan Jurnal Universitas Airlangga Kampus C Mulyorejo Surabaya
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UNIVERSITAS AIRLANGGA DIREKTORAT PENDIDIKAN Tim Pengembangan Jurnal Universitas Airlangga Kampus C Mulyorejo Surabaya
EDITORIAL BOARD Dewan Redaksi SUSUNAN DEWAN REDAKSI MEDIA KEDOKTERAN HEWAN 2006 – 2010 Ketua Penyunting : Epy Muhammad Luqman Sekretaris : Erma Safitri Bendahara : Diah Kusumawati Gali Iklan dan Langganan : Budiarto Penyunting Pelaksana : 1. 2. 3. 4. 5. 6.
Sri Subekti Bendryman Soedjoko Mas’ud Hariadi Fedik Abdul Rantam Suwarno Agus Sudjarwo
Penyunting Teknik : Kusnoto Thomas Valentinus Widiyatno Tata Usaha : Berty Ferijanti
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UNIVERSITAS AIRLANGGA DIREKTORAT PENDIDIKAN Tim Pengembangan Jurnal Universitas Airlangga Kampus C Mulyorejo Surabaya
Table of Contents No 1 2
Title Correlation Between Motility With Viability and Membrane Integrity of Boer Goat Sperm Before Freezing and Post Thawing Peningkatan Kualitas Semen Beku Kambing PE melalui Suplementasi Antioksidan α–tocoferol dalam Pengencer Semen untuk Inseminasi Buatan
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UNIVERSITAS AIRLANGGA DIREKTORAT PENDIDIKAN Tim Pengembangan Jurnal Universitas Airlangga Kampus C Mulyorejo Surabaya Vol. 30 - No. 1 / 2014-01 TOC : 2, and page : 9 - 28 Peningkatan Kualitas Semen Beku Kambing PE melalui Suplementasi Antioksidan α–tocoferol dalam Pengencer Semen untuk Inseminasi Buatan Peningkatan Kualitas Semen Beku Kambing PE melalui Suplementasi Antioksidan α–tocoferol dalam Pengencer Semen untuk Inseminasi Buatan Author : Achadiah Rachmawati |
[email protected] Fakultas Peternakan, Universitas Brawijaya, Malang Sri Wahjuningsih |
[email protected] Fakultas Peternakan, Universitas Brawijaya, Malang Trisna Anjaka | Wahjuningsih
[email protected] Fakultas Peternakan, Universitas Brawijaya, Malang Abstract Abstract Problems often arise in the process of freezing semen is sperm plasma membrane damage, because the membrane contains unsaturated fatty acids are highly susceptible to peroxidation damage. Formation Rective Oxygen Species (ROS) due to damage to the membrane causes a decrease in sperm quality. The purpose of this study was to analyze the supplementation of α-tocoferol in the diluent to maintain the quality of frozen semen to be used for Artificial Insemination (AI). This study used fresh semen Etawah Cross Breed Ram 2 to 2.5 years of age are accommodated once per week with an artificial vagina. Treatment research is the addition of antioxidants α-tocoferol into 100 ml of diluent with a dose of 0, 0.2, 0.4 and 0.6 g. Data were analyzed with randomized block design followed by Duncan's Multiple Range Test. The variables measured were quality of spermatozoa during cooling and thawing again, membrane integrity, the status of capacitation and acrosome reaction, percentage of intact plasma membrane and lipid peroxidation (malonaldehyde or MDA).The results showed that the addition of α-tocoferol 0.4 g/100 ml diluent to give the best results at all stages of freezing (p <0.05), so obtained83,33% pregnancy rate after synchronization of estrus with a double dose of prostaglandin (PGF2α) and IB first. The conclusion of this study is the dose α-tocoferol 0.4 g/100 ml diluent to maintain optimum quality of frozen Etawah Cross Breed Ram. AI applications on Goat PE females were synchronized to produce 76% pregnancy rate. Abstrak Masalah yang sering timbul dalam proses pembekuan semen adalah rusaknya membrane plasma spermatozoa. Pembentukan rective oxygen species (ROS) akibat rusaknya membrane spermatozoa menyebabkan penurunan kualitas spermatozoa. Tujuan penelitian ini adalah untuk menganalisis suplementasi α-tocoferol dalam pengencer terhadap kualitas semen beku yang akan digunakan untuk inseminasi buatan (IB). Penelitian ini menggunakan semen segar kambing Peranakan Etawah (PE) umur 2-2,5 tahun. Perlakuan penelitian adalah penambahan α-tocoferol kedalam 100 ml pengencer AndromedRdengan dosis 0,0; 0,2; 0,4 dan 0,6 g. Data dianalisis dengan analisis varian dan dilanjutkan dengan uji Jjarak berganda Duncan. Variabel yang diamati adalah kualitas spermatozoa pada saat pendingin dan pencairan kembali, yang meliputi integritas membrane, status kapasitasi dan reaksi akrosom, persentase membrane plasma utuh serta kadar lipid peroksidase (Malonaldehid atau MDA). Hasil pengamatan menunjukkan bahwa penambahan α-tocoferol 0,4 g/100 ml pengencer memberikan hasil terbaik pada semua tahapan pembekuan (p<0,05), sehingga didapatkan angka kebuntingan 83,33% setelah sinkronisasi birahi dengan dobel dosis prostaglandin (PGF2α) dan IB pertama. Keyword : prostaglandin, , inseminasi, buatan, , Peranakan, Etawah, , spermatozoa, , Daftar Pustaka : 1. Aebi, H, (1984). Catalase in vitro. Methods Enzymol. 105 : 121-126.. - : 2. Agarwal , S.A. Prabakaran, T.M. Said, (2005). Prevention of Oxidative Stress Injury to
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UNIVERSITAS AIRLANGGA DIREKTORAT PENDIDIKAN Tim Pengembangan Jurnal Universitas Airlangga Kampus C Mulyorejo Surabaya Sperm. Journal of Andrology. Vol 26 (6) : 654-660 . - : 3. Alawiyah D. dan M. Hartono, (2006). Pengaruh Penambahan Vitamin E Dalam Bahan Pengencer Sitrat Kuning Telur Terhadap Kualitas Semen Beku Kambing Boer. J.Indon.Trop.Anim.Agric. 31 [1] March 2006. http://www.deptan.go.id/pdf. Diakses tanggal 20 April 2009. - : 4. Alvarez,J.G. and B.T.Storey, (1995). Differential Incorporation of Fatty Acid into and Peroxidative Loss of Fatty Acids from Phospholipids of Human Spermatozoa. Mol. Reprod.Dev. 42 : 334-345.. - : 5. Arifiantini, R dan T. Yusuf, (2009). Keberhasilan Penggunaan Tiga Pengencer dalam Dua Jenis Kemasan Pada Proses Pembekuan Semen Sapi Frisien Holstein. Departemen Klinik, Reproduksi dan Patologi, Fakultas Kedokteran Hewan.Institut Pertanian Bogor.http:// ejournal.unud.ac.id /abstrak/ arifiantini% 20090302006.pdf. Diakses tanggal 20 April 2009. . - : 6. Bast. A., G.R.M.M. Haenem, and C.J.A. Doelman, (1991). Oxidants and Antioxidants. Stile of Arts. The American J. of Medicine. 91 : 2 – 12.. - : 7. Bilodeau. J.F., S. Chatterjee, M.A. Sorard and C. Gagnon, (2000). Levels of Antioxidants Defences are Decreased in Bovine Spermatozoa After a Cycle of Freezing and Thawing. Mol.Reprod-Dev.55:282-288.. - : 8. Correa. J.R.G, Heersche. Jr and P.M. Zavos, (1997). Sperm Membrane Functional Integrity and Response of Frozen-Thawed Bovine Spermatozoa During Hypo-osmotis Swelling Test Incubation at Raying Temperatures. Theriogenelogy. 47:715-721. . - : 9. Cormier, N, M.A. Sirard, and J. L. Bailey, (1997). Premature Capacitation of Bovine Spermatozoa IsInitiated by Cryopreservation. Journal Andrologi;18:461-468.. - : 10. Dass, N. Den, (1992). Laboratory Assessment of Semen Characteristics. Dalam Steph. J. Dielemant, Ben Colenbrander, Peter Booman and Tette Van der Lende (eds)Clinical Trends and Basic Research in Animal Reproduction.Reprinted from Animal Reproduction Science, Vol.28 Nos. 1-4 (1992. Amsterdam : Elsevier Science Publishers B. V 11. Evans,G dan WMC.Maxwell, (1997). Salomon’s Artificial Insemination of Sheep and Goats. Butterworths, Wellington. - : 12. Fouad, T, (2009). Antioxidants, Nature And Chemistry:Non-Enzymatic Antioxidants Alpha Tocopherol (Vitamin E).www.doctorslounge.com / primary / articles / antioxidants / antioxidants5.htm. - : 13. Gadea. J., FG. Vasquez, C. Matas, J.C. Gardon, S. Canovas and D. Gumbao, (2005). Cooling and Freezing Boar Spermatozoa: Suplementation of The Freezing Media with Reduce Glutaione Preserves Sperm Function . J. of Andrology.6:3.. - : 14. Garner, D.L dan Haves, E.S.E, (2000). Spermatozoa and Seminal Plasma. Dalam Reproduction in Farm Animal. Edisi VII. Diedit oleh Hafes, B dan Hafez, E.S.E. . Lhiladelpia : Lea and Febriger Publising 15. Hamilton,R.J., C.Kalu, E.Prisk,F.B.Padley and H. Pierce, (1997). Chemistry of Free Radicals in Lipids. Food Chemistry 60 : 193-199.. - : 16. Hammerstedt,R.H, (1993). Maintenance of Bioenergenetic Balance in Sperm and Prevention of Lipid Peroxidation : A review of the Effect on Design of Storage Preservation System. Reprod. Fert. Dev 5 :675-765.. - : 17. Hsieh, Y., C.C. Chang and C.S. Lin, (2006). Seminal Malondialdehid Concentration but not Glutathione Peroxidase Activity is Negatively Correlated with Seminal Co. - : 18. Huang,C. R. Niu, J. Wang, (2007). Toxic Effect of Sodiun Fluoride on Reproductive Function in Male Mice. Fluoride 40(3): 162-168.. - : 19. Janice,L., Bailey, N. Cormier, (2000). Semen Cryopreservation in Domestic Animals : A Damaging and Capacitating Phenomenon. Journal of Andrology vol. 21. no.1. - : 20. Jayendra, R.S and L.J.D. Zaneceld, (1984). Instructions for Hypoosmotic Swelling Test (HOS) Test. Semen Analysis. Reproductive Resources Centre. Lab Grant Hospital of Chicago . - : 21. Kaul,G. S.Singhs,K.K. Gandi,and Anand. , (1997). Calcium Requirement and Time Course of Capacitation of Goat Spermatozoa Assisted by CTC assay. Journal of Andrology. 29 (5): 243-251. . :22. Kumar,S., J.D.Millar and P.F.Watson, (2003). The Effect of Cooling Rate on Survival of Cryopreserved Bull,Ram and Boar Spermatozoa : A Comparison of Two Controlled-rate cooling
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UNIVERSITAS AIRLANGGA DIREKTORAT PENDIDIKAN Tim Pengembangan Jurnal Universitas Airlangga Kampus C Mulyorejo Surabaya Anim.Reprod.Sci 48 :267-279. . - : -
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