Curriculum Vitae Ernawati Arifin Giri-Rachman, Ph.D School of Life Sciences and Technology Institut Teknologi Bandung Indonesia Jl. Ganesa 10 Bandung
+62-22-2511575/
+62-22-2534107
[email protected] ,
[email protected]
Position : Assistant Professor in Molecular Biology Academic Career : Assistant Professor in Molecular Biology Institut Teknologi Bandung
2006
Doctorate (Molecular Microbiology)
University of Bristol
2003
Master (Chemistry)
Institut Teknologi Bandung
1997
Bachelor (Chemistry)
Institut Teknologi Bandung
1993
Employment : Academic staff (2006 – now) Research and development projects 1. National Vaccine Consortium in Hepatitis B Insentif Riset Sinas-Kementrian Riset-Teknologi, 2012-…… (Co-Researcher, ITB Coordinator) Development and production of Subunit Hepatitis B Vaccine in Indonesia 2. Riset Centre and Advance Science ITB. 2015. Purification of P-particle and development of intranasal therapeutic and preventive Hepatitis B vaccine 3. Riset Inovasi KK ITB.2015. Studi dimerasisasi PhoR M.tuberculosis untuk pengembangan obat baru. 4. Riset Unggulan ITB.2015. Pengembangan humanized antibodi monoclonal anti-HBsAg
5. Determination of 3-D conformation of Phor domain sensor of Mycobacterium tuberculosis for development of new anti tuberkular. 2014. Funded by Institut Teknologi Bandung 6. Cloning and crystallization of PhoR cytoplasmic domain from Mycobacterium tuberculosis to develop novel multitarget antitubercular drug. 2013. TWAS Grant 7. Pathogenesis and immunology of enteric virus. 2012. Fulbright Visiting Scholar 2012 : in Dr. Lijuan Yuan Laboratory, Virginia Tech, US. 8. Riset dan Inovasi KK ITB, 2012 (Princial Investigator) Production of Modified L-HBsAg in Plant as a Protective Hepatitis B Vaccine Antigen 9. Riset PP Pangan, Kesehatan dan Obat ITB, 2011 (Principle Investigator) Initiation of Hepatitis B Surface Antigen (HBsAg) Production based on Indonesian isolate in Pichia pastoris 10. Riset Hibah Kompetisi Strategis Nasional, DIKTI, 2009-2011 (Principle Investigator) Production of L-HBsAg in plant to solve National Health Problem 11. Riset Unggulan ITB, 2009 (Principle Investigator) Production of Hepatitis B surface antigen (HBsAg) and mutein G145R HBsAg in Pichia pastoris 12. Riset kompetitif LIPI- 2007-2011 (Co-Researcher) Development of Hepatitis B edible vaccine 13. Riset Desentralisasi DIKTI, 2012 (Principal Investigator) Purification and Crystalization of PhoR Sensor Domain Mycobacterium tuberculosis as a target of Novel Antitubercular 14. National Vaccine Consortium in Tuberkulosis, Insentif Riset Sinas-Kementrian Riset-Teknologi, 2012 (Co-Researcher, ITB Coordinator) Development of Prototype of Tuberculosis Vaccine 15. Indonesian Torray Science Foundation, 2009 (Co-researcher) Targetting PhoP-PhoR two component signal transduction to develop a novel high throughput screening system for searching new antibacterial against M . tuberculosis 16. Riset Internasional ITB, 2008, collaboration with School of Pharmacy ITB and University of Groningen, Netherlands (Co-Researcher) Molecular characterization of streptococcal adhesin to human fibronectin using Surface Plasmon Resonance 17. KNAW Mobility Program, collaboration with School of Pharmacy ITB and University of Groningen, Netherlands (Co-Researcher) Drugs development of Streptococcus Pyogenes 18. Joint Research Program Hibah Kompetisi B (PHK-B)-DIKTI, Kerjasama SITH-ITB dan Fakultas Kedokteran UK Maranatha : 2009 (Co-Researcher) Development of FCGamma RIII detection 19. Riset Hibah Kompetitif Strategis Nasional DIKTI, 2009 (Co-Researcher) Development of HCV diagnostic kit 20. Riset Insentif Kementrian Ristek dan Teknologi, 2007 (Co-Researcher) Microsateliate in Penaues Monodon 21. Riset Pembinaan Ilmu Kedokteran (Risbin-Iptekdok), Kerjasama dengan Universitas Hasanudin , 2007 (Konsultan) Overexpression of Cpn-1 as Tuberculosis vaccine candidate 22. Hibah Pekerti DIKTI, Kerjasama dengan Universitas Hasanudin , 2006 (Co-Researcher) VP 28 as White Spot Syndrome Virus vaccine candidate in shrimp 23. Riset Fakultas – ITB Kerjasama dengan Kelompok Keahlian Biokimia – ITB , 2006 (Co-
researcher) Protein analysis of gene expressed in invation process of Salmonella typhi straind Indonesia 24. Riset Pembinaan Ilmu Kedokteran (Risbin-Iptekdok), Kerjasama dengan Universitas Hasanudin, 2006 (Consultant) Immune response variant against Anti-ESAT6 in M akasar 25. Intitation of collaboration with Dr. Claire Leuseur, National University of Singapore , 2005 Site directed mutagenesis of Cholera toxin B subunit to investigate cholera toxin assembly in vitro 26. Indonesian Torray Science Foundation, 2004 ( (Principal investigator) Study of D51 mutant of two component regulatory system of Vibrio cholerae 27. PhD Thesis, 2003 The Cpx two component regulatory system of Vibrio cholerae (Supervisor : Prof.T.R.Hirst, University of Bristol, UK) Cooperation with industry : 1. PT Biofarma (Persero) 2. 2012-now: National vaccine consortium in Hepatitis B Publications
Contribution to GenBank NCBI Database : 1. Giri-Rachman,E.A ., Kocher,J.F., Li,G., Bui,T., Jiang,X. and Yuan,L. Norovirus Hu/GII.4 2006b/092895/2008/USA major capsid protein gene, complete cds.GenBank: KC990829.1. 2013 2. Jinata,C., Giri-Rachman,E.A . and Retnoningrum,D.S. Hepatitis B virus isolate CJ66 small S protein (sHBsAg) gene, partial cds. JQ646056.1-JQ646072, 2012 (18 sequences) Journal : 1. Ernawati Arifin Giri-Rachman, Indah Woro Utami, Shinta Kusumawardani, Debbie Soefie Retnoningrum, Dessy Natalia, Nurfitriani, Gilang Nadia, Patricia Gita Naully and Neni Nurainy. 2015. Construction, Expression and Characterization of Multi Cassettes Encoding Indonesian Small Hepatitis B Surface Antigen (s-HBsAg) in Methylotropic Yeast Pichia pastoris. Biotechnology 14 (5): 225-232 DOI: 10.3923/biotech.2015.225.232 2. 2. OKTIRA ROKA AJI, DYSHELLY NURKARTIKA PASCAPURNAMA, FENRYCO PRATAMA, IHSANAWATI IHSANAWATI, MAELITA RAMDHANI MOEIS, ERNAWATI ARIFIN GIRI-RACHMAN, 2014. Cloning, Overexpression, and Purification of PhoR CytoplasmicDomain Protein from Mycobacterium tuberculosis strain H37Rv. Vol 8, No 4 (2014) 3. 3. Ernawati Arifin Giri-Rachman, Fenryco Pratama, Oktira Roka Aji, Arum Patriati, Ihsanawati, Maelita Ramdani Moeis, Edy Giri-Rachman Putra, Expressionand Purification of PhoR SensorDomain Histidine Kinaseof Mycobacterium tuberculosisin Eschericia coli. Submited to Microbiology Indonesia, under review 4. Ernawati A. Giri-Rachman, Ivan Roostanto, Maelita R. Moeis. Construction and Immune Response Preliminary Study of 1 mpt70 and esxA Fusion Gene. Submitted to Journal of
Mathematical and Fundamental Science ITB 5. Jacob Kocher, Tammy Bui, Erna Giri-Rachman, Ke Wen, Guohua Li, Xingdong Yang,Fangning Liu, Ming Tan, Ming Xia, WeimingZhong, Xi Jiang, and Lijuan Yuan. 2014. Intranasal P particle vaccine protects against human GII.4 norovorus infection and diarrhea in gnotobiotic pigs. J Virol. pii: JVI.01249-14. 6. Ke Wen, Christine Tin, Haifeng Wang, Xingdong Yang,Guohua Li, Ernawati Giri-Rachman , Jacob Kocher, Tammy Bui, Sherrie Clark-Deener, Lijuan Yuan. 2014.Probiotic Lactobacillus rhamnosusGG enhanced Th1 cellular immunity but did not affect antibody responses in a human gut microbiota transplanted neonatal gnotobiotic pig model. PLoS One. 10;9(4):e94504. doi: 10.1371 7. Husen Zhang, Haifeng Wang, Megan Shepherd, Ke Wen, Guohua Li, Xingdong Yang, Jacob Kocher,Ernawati Giri-Rachman , Allan Dickerman, Robert Settlage, Lijuan Yuan. Lactobacillus rhamnosusGG modulates the human gut microbiota in rotavirus vaccinated and challenged gnotobiotic pigs. 2013. Submitted 8. Ugiyadi M, Tan MI, Giri-Rachman EA, Zuhairi FR, Sumarsono SH. The expression of essential components for human influenza virus internalisation in Vero and MDCK cells. Cytotechnology . Published online 2013 Aug 4 9. Martius E., Mardjuki A, Giri-Rachman, E , Riani C, Cahyati , Retnoningrum DS. 2013. Role of Lys59 and Lys386 of streptokinase from type 12 group A Streptococcus on in vitro stability against plasmin cleavage. Journal of Pure and Applied Microbiology , 7: 2617-2627 10. Chandra Jinata, Ernawati Arifin Giri-Rachman and Debbie Soefie Retnoningrum. 2012. Molecular Analysis of Immune Escape Mutants of Hepatitis B Virus from Local Clinical Samples. Microbiology Indonesia. 6 (1), 9-14. 11. FX Reymond Sutandy, Putri Dwi Utari, Tati Kristianti, Sony Suhandono, Amy Estiati and Ernawati A. Giri Rachman. 2012. Transformation and Analysis of L-HBsAg DNA Transient Expression in Tobacco (Nicotiana tabacum Linn.) Seedlings. Journal of Life Sciences and Technology (STM online Journal) School of Life Science and Technology, ITB, 1 (1) : 19-24 12. Zrimi, J., A. N. Ling, E. A. Giri-Rachman, G. Feverati, C. Lesieur., 2010. Cholera Toxin B Subunits Assemble into Pentamers - Proposition of a Fly-Casting Mechanism. Plos One 5 (12) e15347 (eISSN-1932-6203) 13. Aldina S. Suwanto, Ihsanawati and Ernawati Arifin Giri-Rachman. 2011. Molecular Cloning of PhoR Sensor Domain from Mycobacterium tuberculosis for Structure-Based Discovery of Novel AntiTubercular Drugs . Journal of Life Sciences . 5:454-459 14. Sony Suhandono, Rasi Fitria, Ernawati Arifin Giri Rachman . 2010. Sequence Analysis of PutativepotB , potC , and potD Genes from Serratia rubidae . Microbiology Indonesia . 4:2, (ISSN 1978-3477) 15. Patrisia Puspapriyanti, Johan Lucianus, Ernawati A. Giri-Rachman . 2010. Kloning DNA Pengkode PhoQ untuk Pengembangan High Throughput Screening System sebagai Usaha Mencari Antibakteri Baru terhadap Salmonella typhi. Jurnal Kedokteran Maranatha . 9(2): 136-145 (ISSN 1411-9641) 16. Kirby Saputra, Johan Lucianus, Ernawati A. Giri-Rachman. 2009. Deteksi mutasi pada Quinolone Resistance Determining Region (QRDRs) gen gyrA pada Salmonella typhi Isolat Klinik dan Galur khas Indonesia. Jurnal Kedokteran Maranatha 9(1): 1-7 (ISSN 1411-9641) 17. Suhandono S., Giri-Rachman E. A. , I.M Zainuddin, P D Utari, A. Supraba and A. Estiati. 2007. Construction of Binary Vector with Wound Inducible Promoter For HBsAg Expression : Development of Plant–based Edible Hepatitis B Vaccine from Indonesian isolate. Annales
Bogorienses 11 (1): 8-18 (ISSN : 0517-8452). Paten : 1. PLASMID REKOMBINAN YANG BERFUNGSI SEBAGAI VEKTOR EKSPRESI UNTUK PRODUKSI BERLEBIH HEPATITIS B SURFACE ANTIGEN (HBsAg) DI HANSENULA POLYMORPHA. No Pendaftaran : P00201407051. 2014 Paten diajukan bersama ITB dan PT Biofarma (Persero). ITB : Dessy Natalia, Ernawati A. Giri-Rachman (SITH/PP Biosains Bioteknologi ITB), Debbie Soefie Retnoningrum dan Fernita Puspasari Biofarma : Neni Nurainy, Gilang Nadia, Vanny Narita 2. VIRUS-LIKE PARTICLE PROTEIN SMALL HEPATITIS B SURFACE ANTIGEN (sHBsAg) UNTUK BAHAN BAKU VAKSIN HEPATITIS B DAN PROSES PRODUKSINYA DI SEL INANG PICHIA PASTORIS.NO PENDAFTARAN : P00201504908. 12 AGUSTUS 2015 Paten diajukan bersama ITB dan PT Biofarma (Persero) ITB : Ernawati A. Giri-Rachman (SITH/PP Biosains Bioteknologi ITB), Dessy Natalia, Debbie Soefie Retnoningrum, Elvi Restiawaty, Wardono Niloperbowo dan Fernita PuspasariBiofarma : Neni Nurainy, Gilang Nadia, Dicky Mahardhika
Proceeding : 1. Langi, Gladys Emmanuella Putri ; Moeis, Maelita R. ; Ihsanawati, GiriRachman, Ernawati Arifin , Molecular cloning and cold shock induced overexpression of the DNA encoding phor sensor domain from Mycobacterium tuberculosis as a target molecule for novel antitubercular drugs, AIP Conference Proceedings, Volume 1589, Issue 1, p.407-410 2. Dina Rachmi Ramdhani, Putri Dwi Utari, Ima Mulyama Zainuddin, Ami Estiati, Sony Suhandono,Ernawati A. Giri-Rachman. 2009. Construct DNA Encoded Large Hepatitis B Surface Antigens (L-HBsAg) from Indonesian Isolates into Expression of Vector pCAMBIA 1390 to Development of The Third Generation Vaccine. Proceeding of International Conference and Exhibition on Biomass Production , School of Life Sciences and Technology, ITB. ISBN 978-60296488-0-5 3. Ima Mulyama Zainuddin , Sony Suhandono, Ernawati Arifin Giri-Rachman , Ami Estiyati . 2007. “Construction of Binary Vector with MeEF1 Promoter for Gene Expression of Hepatitis B SurfaceAntigen (HBsAg) in Plant.” Proceeding in International Seminar on Pharmaceutics. School of Pharmacy Institut Teknologi Bandung. 4. Saripah Sundari, Junessa Palao, and Ernawati Giri-Rachman . 2009. Targetting PhoP-PhoR two component signal transduction to develop a novel high throughput screening system for searching new antibacterial against M . tuberculosis. Proceeding of International Conference and Exhibition on Biomass Production , School of Life Sciences and Technology, ITB . ISBN 978-602-96488-0-5 5. Mufti Hatur Rahmah and Ernawati A. Giri-Rachman . 2009. Amplification of truncated MprB encoding gene to Develop a Novel High ThroughputScreening System for Searching New Antibacterial against Mycobacterium Tuberculosis. Proceeding of International Conference and Exhibition on Biomass Production, School of Life Sciences and Technology . ISBN 978-602-96488-
0-5 6. Ima Mulyama Zainuddin, Ima, Sony Suhandono, Ernawati A. Giri-Rachman , Amy Estiati.2008. Isolation of Poly-A vspB Fragment from Soybean cv. Slamet, Proceeding in International Conference of Mathematical and Natural Sciences 7. Ernawati Arifin Giri-Rachman , Thomas Robertus and Sarwono Hadi. 2006. “PCR for detectionSalmonella typhi strain Ind onesia.” Proceeding in International Conference of Mathematical and Natural Sciences. Award : I.
Poster/Oral Presentation :
1. Ernawati Arifin Giri-Rachman, Indah Woro Utami, Shinta Kusumawardani, Debbie Soefie Retnoningrum, Dessy Natalia, Nurfitrian, Patricia Gita Naully, Neni Nurainy, Construction, Expression and Characterization of Multi Copy Expression Cassete of HBsAg in Pichia pastoris. 7-8 Mei 2015. International Seminar : Infectious Diseases, Biochemistry and Structural Biology. Molecular and Cellular Life Science (MCLS) : Surabaya. The Best Presenter in Infectious Disease 2. Utari, P.D. , Suhandono. S., Ramadhani, D.R., Sutandy, FX.R., Badriyah, L., Kristianti, T., Estiati, A., dan Giri Rachman, E.A ., Construction and Transient Expression of Recombinant Large Hepatitis B Surface Antigen in Plants to Develop Third Generation Vaccine. Proceeding of The 5th International Eijkman Conference on the Eijkman Institute comes of age: vitamins, genomics, and welfare , 8-10 November 2011 . Best Oral Presentation 3. Dina Rachmi Ramdhani , Putri Dwi Utari, Ima Mulyama Zainuddin, Ami Estiati, Sony Suhandono,Ernawati A. Giri-Rachman. Construct DNA Encoded Large Hepatitis B Surface Antigens (L-HBsAg) from Indonesian Isolates into Expression of Vector pCAMBIA 1390 to Development of The Third Generation Vaccine. International Conference and Exhibition on Biomass Production, School of Life Sciences and Technology, ITB, November, 2009 . 2 nd Best Poster Presentation 4. Caroline T. Sardjono, Teresa L. Wargasetia, Ernawati G. Rachman , Marselina I. Tan. DETECTION OF FC GAMMA RECEPTOR III EXPRESSION IN HEMATOPOETIC STEM CELL, International Seminar and Workshop, 8th Basic Molecular Biology Course on Stem Cell, Treatment With Stem Cell. Brawijaya University Malang. May 2010, Best Poster Presentation 5. Junessa Palao, Saripah Sundari and Ernawati A.Giri-Rachman , Cloning of Promoter iclR E.coli K-12 in Vector pRSET with eGFP probe : Application Promoter iclR to develop Screening System for Searching New Antibiotic Against MDR-TB (Multi Drugs Resistant Tuberculosis), Kongres Nasinal VII PAMKI Pengendalian Penyakit Infeksi untuk meningkatkan kualitas hidup bangsa, 12 - 14 November 2009. Best poster presentation 6. Saripah Sundari, Junessa Palao, and Ernawati Giri-Rachman , Targetting PhoP-PhoR two component signal transduction to develop a novel high throughput screening system for searching new antibacterial against M . tuberculosis. Presentasi oral pada “Biotechnology for a Sustainable Future” Universitas Udayana, Bali, 15-16 September 2009. 2nd Best Oral Present ation 7. Kirby Saputra , Johan Lucianus, Ernawati A. Giri-Rachman. Deteksi mutasi pada Quinolone Resistance Determining Region (QRDRs) gen gyrA pada Salmonella typhi Isolat Klinik dan Galur khas Indonesia. Seminar Fakultas Kedokteran Universitas Kristen Maranatha, July 2009. Best poster presention 8. Mohammad Bahrelfi Belafiff, Ernawati A. Giri-Rachman , Adi Pancoro. Phylogeography and
distribution pattern of H5N1 from Indonesia, the Second International Conference on Mathematics and Natural Sciences (ICMNS). Institut Teknologi Bandung. Best Oral Presentation . October 2008 II. FULBRIGHT SENIOR RESEARCH PROGRAM , 2012 Conference: 1. Ernawati Arifin Giri-Rachman, Indah Woro Utami, Shinta Kusumawardani, Debbie Soefie Retnoningrum, Dessy Natalia, Nurfitriani, Patricia Gita Naully, Neni Nurainy, Construction and Expression of Multi Cassettes Encoding Indonesian Small Hepatitis B Surface Antigen (s-HBsAg) in Methylotrophic Yeast Pichia pastoris. 1st Asean Microbial Biotechnology Conference. Thailand 2. Febiana Rima Diputri, Ernawati A. Giri-Rachman, Konstruksi dan Overekspresi Fusi Terlarut Maltose Binding Protein (MBP) dan Domain Sitoplasmik Protein PhoR Mycobacterium tuberculosis sebagai Pengembangan Target Anti Tuberkulosis Baru. PIT PERMI 8-9 Oktober. 2015. Semarang 3. Meliawati Poniman, Ernawati A. Giri-Rachman, Stephanie Lie, Debbie Soefie Retnoningrum, Lijuan Yuan. Cloning and Purification of Norovirus P Particle and in silico Study as an Intranasal Hepaititis B Vaccine Candidate. PIT PERMI 8-9 Oktober. 2015. Semarang 4. Ricky Mandala Putra, Irma Sopiyani Kurnia dan Ernawati Arifin Giri-Rachman. Purifikasi Protein Domain Sitoplasmik PhoR Mycobacterium tuberculosis H37Rv untuk penapisan Antituberkulosis Baru. PIT PERMI 8-9 Oktober. 2015. Semarang 5. Shely Agustina, Vanny Narita, Ernawati Arifin Giri-Rachman, Siti Isnaeni Mutmainah, Dessy Natalia, Debbie Soefie Retnoningrum, Neni Nurainy. Clonind and Expression lf L-HBsAg Gene in Pichia pastoris GS 115. PIT PERMI 8-9 Oktober. 2015. Semarang 6. Mellysa Rahmita dan Ernawati A. Giri-Rachman. Pengembanga Sistem Penapisan Obat Antituberkulosis Baru dengan Target PhoR Mycobacterium tuberculosis H37 Rv Menggunakan Protein Repressor araC Escherichia coli. PIT PERMI 8-9 Oktober. 2015. Semarang 7. Nur Amalia Rosyada. dan Ernawati A. Giri-Rachman. Konstruksi dan Overekspresi Fusi Maltose Binding Protein dan Domain Sensor Protein PhoR Mycobacterium tuberculosis. PIT PERMI 8-9 Oktober. 2015. Semarang 8. Prima Nanda Fauziah, Sony Suhandono, Ernawati A. Giri-Rachman. Molecular Cloning, Characterization, Promoter Analysis of the Zinc Finger 23 (ZIF23) Gene to Deveop a Novel High Throughput Screening System or New Antitubercular by Targetting PhoR of Mycobacterium tuberculosis. PIT PERMI 8-9 Oktober. 2015. Semarang 9. Risma Wiharyanti, Made Tri Ari Penia Kresnowati, Ernawati Arifin Giri-Rachman , ISOLATION And CLONING OF PROMOTER FORMATE DEHYDROGENASE (FDH) FROM Candida orthopsilosi. INTERNATIONAL SEMINAR AND MINI WORKSHOP ON PHARMACEUTICS. School of Pharmacy ITB. Advanced Research in Pharmaceutical Science and Technology. 2015 10. Maharani Ugayadi, Marselina I Tan, Ernawati A Giri-Rahman , Fawzi R Zuhairi, Sony H Sumarsono. Primary monkey trachea cell culture express the subtantial components for influenza virus internalization. International Seminar on Tropical Bioresources for Sustainable Bioindustry. ITB Bandung, 30–31 October 2012 11. Regina Angeline, Rahma Tejawati Maryama, Ernawati Arifin Giri-Rachman and Wardono Niloperbowo. Mathematical Modelling of Growth of Recombinant Pichia pastoris , Producing sHBsAg, in Yeast Extract Potato Dextrose Medium (YEPD) and Buffered Glycerol-complex Medium (BMGY). International Conference on Mathematical and Natural Science . Institut Teknologi Bandung. 8-9 November 2012. Oral Presentation
12. Amy Estiati, Ade Nena, Ernawati A. Giri-Rachman , Putri Sandy Pangestu, Putri Dwi Utari, Ima Mulyama and Sony Suhandono. Transformation of Hepatitis B Surface Antigen into Local Banana Plants for Molecular Farming Vaccine. International Conference on Biomedical Science. Oral Poster Presentation. School of Life Science and Technology, Institut Teknologi Bandung. 27-28 February 2012. Oral Poster Presentation. 13. Rahma Tejawati Maryama, Shinta Kusumawardhani, Dessy Natalia, Debbie S Retnoningrum and Ernawati A. Giri-Rachman . Intracelullar Expression of HbsAg Genotype B in Pichia pastoris KM71 Hyperresistant Zeosin, International Conference on Biomedical Science . School of Life Science and Technology, Institut Teknologi Bandung. 27-28 February 2012. Oral Poster Presentation. 14. Putri Dwi Utari, Dina Rachmi Ramadhani, FX Reymond Sutandy, Lailatul Badriyah, Tati Kristanti, Ami Estiati, Sony Suhandono and Ernawati Arifin Giri-Rachman . Construction and Transient Expression of Recombinant Large Hepatitis B Surface Antigen (L-HBsAg) in Plants to Develop Third Generation Hepatitis B Vaccine. International Conference on Biomedical Science . School of Life Science and Technology, Institut Teknologi Bandung. 27-28 February 2012. Oral Poster Presentation. 15. Kusumawardani, S, Huvat, E.A, Natalia, D, Retnoningrum, D.S , and Giri-Rachman, E.A . Cloning of HBsAg Encoded Gene From Indonesian Isolate in Pichia pastoris KM71. The Third GruberSoedigdo Lecture. ITB and University of Groningen. 27-29 July 2010. Poster Presentation. 16. Dina Rachmi Ramdhani and Ernawati Arifin Giri Rachman , Cloning of Large Hepatitis B surface antigens (L-HBsAg) from Indonesian Isolates : Development of the Third Generation Vaccine. Presentasi oral pada “International Conference on Biological Science” Universitas Gadjah Mada, Yogyakarta 16-17 Oktober 2009 17. Putri Dwi Utari, Dina Rahmi Ramdani, Ima Mulyama, Sony Suhandono, Ernawati A. GiriRachman, Amy Estiati, C loning and construction of plant binary vector for expression of s HB s A g and L-HBsAg regulated by wound inducible promoter Presentasi oral pada “Biotechnology for a Sustainable Future” Universitas Udayana, Bali, 15-16 September 2009 18. Emeraldo A. Huvat, Shinta Kusumawardhani, Ernawati A. Giri-Rachman , Debbie S. Retnoningrum, Dessy Natalia, Cloning Open Reading Frame (ORF), Hepatitis B Surface Antigen (HBsAg) encoding gene in pGEM-T-EASY For Further Construction In Pichia pastoris, Poster Presentation. International Conference and Exhibition on Biomass Production, School of Life Sciences and Technology, ITB, 25-26 November, 2009 19. Ernawati A. Giri-Rachman dan Dina Rachmi Ramdhani, ‘Cloning DNA Encodes Large HBsAg of Wild type and T143M Vaccine escape Mutant Hepatitis B Virus from Indonesian Isolate', Oral Presentation, Malaysian of Molecular Biology and Biotechnology. August, 2009 20. Zainuddin I.M, Suhandono, S., Giri-Rachman , E.A. Estiati A. ¨Progress on construction of plant binary vector for expression of hepatitis B surface antigen (HBsAg) Mutant T140I controlled by wounding induction. The First International Symposium on Molecular Pathogenesis. School of Pharmacy Institut Teknologi Bandung. Poster Presentation. January 2008 21. Supraba, A, Suhandono S, Giri-Rachman E.A. , Kusumawati, D, Hidayat, T, Parwati I, and Estiati A,¨Construction of fusion hepatitis B virus surface antigene gene isolated from serum samples of Hasan Sadikin Hospital patient and VSPaS signal peptide. The First International Symposium on Molecular Pathogenesis. School of Pharmacy Institut Teknologi Bandung. Poster Presentation. January 2008 . 22. Gladys Langi, Ihsanawati, Maelita R. Moeis and Ernawati A. Giri-Rachman. Molecular Cloning and Cold Shock Induced Overexpression of the DNA Encoding PhoR Sensor Domain
from Mycobacterium tuberculosis as a Target Molecule for Novel Anti-Tubercular Drugs.International Conference on Mathematical and Natural Science . Institut Teknologi Bandung. 8-9 November 2012. Oral Presentation. 23. Dyshelly Nurkartika, Ernawati A. Giri Rachman , Maelita R. Moeis and Ihsanawati. Cloning and Overexpression of PhoR Cytoplasmic Domain Mycobacterium tuberculosis H37Rv as A Molecular Target for Novel Antitubercular Drugs. International Conference on Mathematical and Natural Science . Institut Teknologi Bandung. 8-9 November 2012. Oral Presentation. 24. Irma Sopiyani and Ernawati A. Giri-Rachman . PhoR of Mycobacterium tuberculosis as a Target for High Throughput Screening System of Novel Anti-Tubercular Drugs. International Conference on Mathematical and Natural Science . Institut Teknologi Bandung. 8-9 November 2012. Oral Presentation. 25. Irma Sophie, Saripah Sundari, Junessa Palao Kurniadi and Ernawati A. Giri-Rachman . PhoR of Mycobacterium tuberculosis as a Target for High Throughput Screening System of Novel AntiTubercular Drugs. International Conference on Biomedical Science. School of Life Science and Technology, Institut Teknologi Bandung. 27-28 February 2012. Oral Poster Presentation. 26. Aldina S. Suwanto, Arkasha Sadewa, Gladys Langi, Ihsanawati and Ernawati A.Giri-Rachman . Characterization, Molecular Cloning and Expression of the DNA Encoding PhoR Sensor Domain from Mycobacterium tuberculosis as a Target Molecule for Novel Anti-Tubercular Drugs. International Conference on Biomedical Science . School of Life Science and Technology, Institut Teknologi Bandung. 27-28 February 2012. Oral Poster Presentation. 27. Inri Kusumadewi, Lidya, Ernawati Giri Rachman , Bachti Alisjahbana, Ida Parwati and Reinot van Crevel. Molecular Epidemiology Study of Mycobacterium tuberculosis in Java and Papua. International Conference on Biomedical Science . School of Life Science and Technology, Institut Teknologi Bandung. 27-28 February 2012. Oral Poster Presentation. 28. Purwaeni, Ernawati A. Giri-Rachman and Debbie S Retnoningrum. Role of Residues 256-257 and 278 in ß Domain on Plasminogen Activation of Streptokinase of Streptococcus pyogenes. International Conference on Biomedical Science. School of Life Science and Technology, Institut Teknologi Bandung. 27-28 February 2012. Oral Poster Presentation. 29. Efrida Martius, Ernawati A. Giri-Rachman , Yeyet Cahyati and Debbie Soefie Retnoningrum. 2012. Alanine-scanning mutagenesis identifies residues ß Domain of Streptokinase of Streptococcus pyogenes involved in Plasminogen Activation. International Conference on Biomedical Science . School of Life Science and Technology, Institut Teknologi Bandung. Oral Poster Presentation. 30. Ali B Kusuma, Anggie PN Hidayati, Ernawati A. Giri-Rachman , Din Syafruddin, Hisao Yoshikawa and Masaharu Tokoro. Trichomonads Species Distributing in Southwest Sumba District, Indonesia. International Conference on Biomedical Science . School of Life Science and Technology, Institut Teknologi Bandung. 27-28 February 2012. Oral Poster Presentation. 31. Saripah Sundari dan Ernawati Giri-Rachman , Pengembangan Sistem Screening Antibakteri Baru dengan Target Gen PhoR-PhoP pada M.tuberculosis dalam Usaha Penanggulangan Resistensi Antibiotik pada Pengobatan Tuberculosi, Parade Penelitian Tuberkulosis II, 27-29 April 2010, Unit Penelitian Kedokteran UNPAD- RS Hasan Sadikin, Bandung. Poster presentation 32. Aldina Suwanto dan Ernawati A. Giri-Rachman , Karakterisasi Sensor Domain PhoRMycobacterium tuberculosis, Structure Based Drug Discovery Obat Anti Tuberkular Baru Berbasis Biodiversitas Indonesia dengan Target Sistem Transduksi Sinyal , Parade Penelitian Tuberkulosis II, 27-29 April 2010, Unit Penelitian Kedokteran UNPAD- RS Hasan Sadikin, Bandung. Poster presentation 33. Junessa Palao Kurniadi dan Ernawati A. Giri-Rachman . Kloning Promoter dari Protein Represor
IclR E.coli K-12 dalam Vektor pRSET dengan Gen Penanda EGFP : Pengembangan Sistem Skrining Antitubercular Baru untuk Mengatasi Multidrug-Resistant Tuberculosis (MDR-TB). Parade Penelitian Tuberkulosis II, 27-29 April 2010, Unit Penelitian Kedokteran UNPAD- RS Hasan Sadikin, Bandung. Poster presentation 34. Soni Muhsinin, Lidya, Ernawati Giri Rachman , Ida Parwati, Pengembangan Metode Molekuler untuk Deteksi Mycobacterium tuberculosis yang Resisten terhadap Parade Penelitian Tuberkulosis II, 27-29 April 2010, Unit Penelitian Kedokteran UNPAD- RS Hasan Sadikin, Bandung. Oral presentation 35. Tina Kusumaningrum, Lidya, Ernawati Giri Rachman , Ida Parwati, PENGEMBANGAN METODE REAL TIME PCR UNTUK MENDETEKSI MUTASI katG KODON 315 PADAMycobacterium tuberculosis, Parade Penelitian Tuberkulosis II, 27-29 April 2010, Unit Penelitian Kedokteran UNPAD- RS Hasan Sadikin, Bandung. Poster presentation 36. Putri Dwi Utari, Maelita Ramdani Moeis, Ernawati A. Giri-Rachman , Sony Suhandono, Amy Estiati, Ima Mulyama Zainuddin, Apsari Supraba, C onstruction of HBsAg gene with signal peptide Vsp a and cloning into pAF vector and codon optimization of hbsag gene based on musa sp. codon bias in silico. International Conference of Mathematical and Natural Sciences.October 2008. Poster presentation 37. Rostinawati, T.C.K.hendarto, A. Mulyono, N.T. Pasaribu, E.A Giri-Rachman , and D. S Retnoningrum,¡¨Identification of mutations in Hepatitis B virus Polymerase Gene from Hepatitis B patients from several cities in Java. The First International Symposium on Molecular Pathogenesis. School of Pharmacy Institut Teknologi Bandung. Poster Presentation. January 2008 38. Rosana Agus, Anandayu Pradita, Ernawati A. Giri-Rachman and Debbie S.Retnoningrum, Cloning open reading frame (orf) of early secretory antigenic target-6 (esat-6) encoding gene amplified from Mycobacterium tuberculosis INDONESIAN ISOLATE IN Eschericia coliDH5a. Poster Presentation. International Conference and Exhibition on Biomass Production, School of Life Sciences and Technology, ITB, 25-26 November, 2009. 39. Ernawati Arifin Giri-Rachman , Tuberculosis and Environment,, Environmental Science Course for Young Researchers Invited from ASEAN Countries 2009, Kanazawa University Symposium 2009, 10 September 2009 40. Ernawati Giri-Rachman Arifin , Improvement of drug discovery system using bacterial protein signalling, Oral presentation, 12 Januari 2009, LAPTH, Annecy , Perancis 41. Agus, R., Kwenang A., D.S. Retnoningrum and Ernawati Arifin Giri-Rachman ,¨Nucleotide sequences of ESAT-6 gene from Indonesian Mycobacterium tuberculosis isolates are higly conserved. The First International Symposium on Molecular Pathogenesis. School of Pharmacy Institut Teknologi Bandung. Poster Presentation. January 2008 42. Ernawati Arifin Giri-Rachman and Fernita Puspasari “Study of D51 mutant of Cpx two component regulatory system in Vibrio cholerae ”, ITSF Seminar, February 2005 43. Ernawati Arifin Giri-Rachman , Lolke de-Haan, Claire Lesieur, Fernita Puspasari and Timothy R.Hirst, “The role of Cpx two component regulatory system in Vibrio cholerae” , International Biotechnology Conference, Bali, December 2004. 44. Ernawati Arifin Giri-Rachman , Lolke de-Haan, Kurshid Nurmahomed, Timothy R.Hirst “Cpx dan pengendalian faktor virulensi di Vibrio cholerae ”, PIT PERMI, 2003. 45. Ernawati Arifin Giri-Rachman , Lolke de-Haan, Claire Lesieur, Timothy R.Hirst” Cpx two component regulatory system di Vibrio cholerae ”, Seminar Jurusan Kimia ITB, 2003 46. Ernawati Arifin Giri-Rachman, “The Cpx two component regulatory system of Vibrio cholerae ”.
PhD Thesis, 2003. Lectures (last 5 years): 1. 2. 3. 4. 5. 6. 7. 8.
Basic Molecular and Cell Biology (For Microbiology Study Program) Applied in Molecular and Cell Biology (For School of Pharmacy) Diagnostic Microbiology Microbial physiolgy (practical) Microbial pathogenesis and Immunology Project on Microbial Genetics Virology Current Issues in Biotechnology
Professional Organization 1. Society of Indonesian Microbiology, Bandung Branch Chairman Juni 2011-............... 2. Society of Indonesian Microbiology, Bandung Branch Program Coordinator 2005-2011 3. Society of Indonesian Biochemistry and Molecular Biology, Bandung Branch Member 2011-...................... Reviewer : Indonesian Torray Science Foundation Research Grant, 2006, 2008 Editor Editorial Team : Microbiology Indonesia (2012-……)
