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Weiss, W.P., J.S. Hogan, K.L. Smith and K.H. Hoblet. 1990. Relationships among selenium, vitamin E and mammary gland health in commercial d a i j herds. J Dairy Sci. 73:381-390. West, ES and W.R. Todd. 1961. Texbook of Biochemistry. 3rded. The MacMillan Company, New York. Wichtel, J.J., A.L. Craigie, D.A. Freeman, H. Varela-alvarez and N.B. Williamson. 1996. Effect of selenium and iodine supplementation on growth rate and on thyroid and somatotropic in dairy calves at pasture. J. Dairy Sci. 79 : 1865-1872. Wilde, C.J. and C.H. Knight. 1989. Metabolic adaptations in mammary gland during the declining phase of lactation. J. Dairy Sci. 72 : 1679-1692. Williams, C.M. and J. W.T. Dickerson. 1990. Nutrition and cancer-some biochemical mechanisms. Nutr. Res.Rev. 3 : 75-100. William, C.H. 1985. Milk collection. In Lactation. Larson, B.L. ed. Iowa State University Press. Ames p. 198-228. Windisch, W., S. Gabler and M. Kirchgessner. 1998. Effect of selenite, selenocysteine and selenomethionine on the selenium metabolism of Se labeled rats. J.Anim. Physio.Anim. Nutr. 78 :67-74. Wikantadi, B. 1977. Biologi Laktasi. Bagian Ternak Perah. Fakultas Peternakan, UGM. Yogyakarta. Whanger, P.D., N.D. Pedersen, J. Hatfield and P.H. Weswig. 1976. Absorption of selenite and selenomethionine from ligated digestive tract segments in rats. Proc. Sco. Exper. Biol. Med. 153:295. Wray, M.I., W.M. Beeson, T.M. Perry, M.T. Mohler and E. Baough. 1979. Effect of soybean, feather and hair meals and fat on the performance of growing-finishing beef cattle. J.Anim.Sci. 48 : 748-757. Wright, T.C., S. Moscardini, P. H. Luimes, P. Susmel and B.W. McBride. 1998. Effect of rumen-undegradable protein and feed intake on nitrogen balance and milk protein production in dairy cows. J. Dairy Sci. 8 1:784-793.
Lampiran 1. Anova Pengaruh Selenoproteinat dalam Ransum Sapi Perah Laktasi pada Kecernaan Bahan Kering (%) in vitro Sumber Keragaman
db
JK
KT
F
F.05
F.O1
Perl.
KBK
Signifikasi
Blok
2
5.71
2.852
1.65
4.46
8.65
A
51.40
a
Perlakuan
4
348.01
87.00
50.32
3.84
7.01
B
59.60
b
AB vs CDE
1
206.66
206.7
119.52
5.32
11.26
C
61.70
c
3
A vs B
1
99.878
99.88
57.76
5.32
11.26
D
66.10
d
CE vs D
1
41.405
41.41
23.95
5.32
11.26
E
61.50
e
E vs C Error Total
1
0.06
0.06
0.03
5.32
11.26
8
13.83
1.73
14
367.54
Lampiran 2. Anova Pengaruh Selenoproteinat dalam Ransum Sapi Perah Laktasi pada Kecernaan Bahan Organik (%) in vitro Sumber Keragaman
db
JK
KT
F
F.05
F.O1
Perl.
KBO
Signifikasi
Blok
2
3.19
1.59
0.89
4.46
8.65
A
52.9
a
Perlakuan
4
329.54
82.38
45.90
3.84
7.01
B
60.3
b
AB vs CDE
1 202.62
202.62
112.89
5.32
11.26
C
62.6
c
Error
8
Total
14.36
14 347.08
1.79
Lampiran 3. Anova Pengaruh Selenoproteinat dalam Ransurn Sapi Perah Laktasi pada NHs (mM) in vitro Sumber db Keragaman
JK
KT
F
F.05
Perl.
F.01
Signifikasi
NH3
Blok
2
0.09
0.05
0.23
4.46
8.65
A
2.20
a
Perlakuan
4
10.1 1
2.53
12.43
3.84
7.01
B
2.43
a
ABC vs DE
1
7.61
7.61
37.42
5.32
11.26
C
3.02
a
Error
8
1.63
0.20
Total
14
11.83
Lampiran 4. Anova Pengaruh Selenoproteinat dalam Ransum Sapi Perah Laktasi pada VFA (mM)in vilro Surnber db Keragaman
JK
KT
Blok
2
153.73
Perlakuan
4
ABC vs DE
F 76.87
F.05
F.O1
Perl.
VFA Signifi kasi
1.10
4.46
8.65
A
121
a
4846.27
121 1.57 17.34
3.84
7.01
B
127
a
1
4737.88
4737.88 67.81
5.32
11.26
C
128
a
Error
8
558.93
Total
14
5558.93
69.87
Lampiran 5. Anova Pengaruh Selenoproteinat dalam Ransum Sapi Perah Laktasi pada Konsumsi Bahan Kering (kglhari) Sumber db Keragaman
JK
KT
F
F.05
F.O1
Perl.
Kons. BK
Signifikasi
Blok
2
11.84
5.92
13.94
4.46
8.65
A
11.8
a
Perlakuan
4
14.75
3.69
8.68
3.84
7.01
B
14.5
c
AC vs BDE
1
10.58
10.58
24.92
5.32
11.26
C
13.20
b
E vs B
1
0.07
0.06
0.19
5.32
11.26
Error
8
3.40
0.42
Total
14
29.99
Lampiran 6. Anova Pengaruh Selenoproteinat dalam Ransurn Sapi Perah Laktasi pada Konsumsi Protein Kasar (kglhari) Sumber db Kerayaman
JK
KT
F
F.05
F.01
Perl.
Kons.
PK
Signilikasi
Blok
2
0.06
0.03
1 .I0
4.46
8.65
A
1.54
a
Perlakuan
4
0.39
0.10
3.63
3.84
7.01
B
1.90
b
ACvsBDE
1
0.28
0.28
10.59
5.32
11.26
C
1.78
a
D vs BE
1
0.00
0.00
0.14
5.32
11.26
E
2.01
b
BvsE
1
0.02
0.02
0.58
5.32-
11.26
Error
8
0.21
Total
14
0.66
Lampiran 7. Anova Pengaruh Selenoproteinat dalam Ransum Sapi Perah Laktasi pada Produksi Susu, 4 % FCM (kg)
.
Sumber db Keragaman
JK
KT
F.
F.05
.-
F.O1
Perl.
4% FCM
Sighifikasi
A
15.10
a
B
18.20
b
11.26
C
17.00
a
11.26
D
20.90
c
Blok
2
71.67
35.84
21.90
4.46
8.65
Perlakuan
4
56.62
14.16
8.65
3.84
7.01
AC vs BDE
1
38.22
38.22
23.36
5.32
A vs C
1
5.82
5.82
3.56
5.32
Error
8
13.09
1.64
Total
14
141.38
'
Lampiran 8. Anova Pengaruh Selenoproteinat dalam Ransum Sapi Perah Laktasi pada Produksi Kolostrum (kghari) Sumber Keragaman
db
JK
KT
F
F.05
F.O1
Perl.
Kolt. (kg)
Signifikasi
Blok
2
71.67
35.84
21.90
4.46
8.65
A
10.1
a
Perlakuan
4
56.62
14.16
8.65
3.84
7.01
B
13.9
b
AC vs BDE
1
38.22
38.22
23.36
5.32
11.26
C
11.2
a
A vs C
1
5.82
5.82
3.56
5.32
11.26
D
12.5
c
DE vs B
1
12.04
2.04
7.36
5.32
11.26
E
12.0
b
E vs D
1
0.54
0.54
0.33
5.32
11.26
Error
8
13.09
1.64
Total
14
141.38
Lampiran 9. Anova Pengaruh Selenoproteinat dalam Ransum Sapi Perah Laktasi pada Globulin Kolostrum (g/hari) Sumber Keragaman
db
JK
KT
F
F.05
F.O1
Perl.
Globl. Signifikasi
Blok
2
0.104
0.052
12.86
4.46
8.65
A
236.67
a
Perlakuan
4
0.096
0.024
5.95
3.84
7.01
B
276.19
a
Error
8
0.028
0.004
Total
14
0.229
Larnpiran 10. Anova Pengaruh Selenoproteinat dalam Ransum Sapi Perah Laktasi pada Bahan Kering Susu (kg/hari) Sumber Keragaman
db
JK
KT
F
F.05
F.O1
Perl.
BK susu
Signifikasi
Blok
2
0.725
0.362
17.20
4.74
9.55
A
1.69
a
Perlakuan
4
0.163
0.041
1.94
4.12
7.85
B
1.84
a
ABCvsDE
1
0.124
0.124
5.87
5.59
12.25
C
1.72
a
AC vs B
1
0.003
0.003
0.16
5.59
12.25
D
2.45
b
AvsC
1
0.001
0.001
0.05
5.59
12.25
E
2.02
b
E vs D
1
0.035
0.035
1.67
5.59
12.25
Error
7
0.147
0.021
Total
13
1.035
Lampiran 11.Anova Pengaruh Selenoproteinatdalam Ransurn Sapi Perah Laktasi pada Bahan Kering Tanpa Lemak Susu (kgthari) Sumber db Keragaman
JK
KT
F
Blok
2
0.007
0.004
0.03
4.74
9.55
A
1.07
a
Perlakuan ABC vsDE
4
0.486
0.122
1.11
4.12
7.85
B
1.13
a
1
0.369
0.369
3.37
5.59
12.25
C
1.03
a
AC vs B
1
0.014
0.014
0.12
5.59
12.25
D
1.53
a
E vs D Error
1
0.101
0.101
0.92
5.59
7
0.766
0.109
13
1.259
Total
F.O1
F.05
.--
Perl.
BK susu
Signifikasi
12.25
Lampiran 12. Anova Pengaruh Selenoproteinat dalam Ransum Sapi Perah Laktasi pada Lemak Susu (ghari) Sumber Keragaman
db
JK
KT
F
F.05
F.O1
Perl.
Lema Signiksusu fikasi
Blok
2 30.554
15.277
2.56
4.46
8.65
A
617.3
a
Perlakuan ABC vs DE
4 49.920
12.480
2.09
3.84
7.01
B
706.1
a
1
32.71
32.71
5.47
5.32
11.26
C
689.8
a
A vs BC
1
4.59
4.59
0.77
5.32
11.26
D
920.6
b
C vs B
1
0.13
0.13
0.02
5.32
11.26
E
756.9
b
E vs D Error
1
12.50
12.50
2.09
5.32
11.26
8
47.81
5.98
14
128.28
Total
Lampiran 13. Anova Pengaruh Selenoproteinat dalarn Ransum Sapi Perah Laktasi pada Protein Susu (ghari) Sumber db Keragaman
JK
KT
Blok
2
0.050
0.025
19.28
4.74
9.55
A
513.9
a
Perlakuan
4
0.048
0.012
9.27
4.12
7.85
B
742.6
b
A vs BCDE
1
0.045
0.045
34.95
5.59
12.25
C
679.9
b
CD vs BE
1
0.002
0.002
1.66
5.59
12.25
D
698.1
b
C vs D
1
0.001
0.001
0.37
5.59
12.25
E
721.9
b
E vs B
1
0.000
0.000
0.1 1
5.59
12.25
Error
7
0.009
0.001
F
F.05
F.01
Perl.
Prot. susu
Signifikasi
Lampiran 14. Anova Pengaruh Selenoproteinat dalam Ransum Sapi Perah Laktasi pada Laktosa Susu (glhari) Sumber Keragarnan
db
JK
KT
F
F.05
F.O1
Perl.
Lakto Signi sa fikasi
Blok
2
159617.8
79808.9
5.78
4.74
9.55
A
614.75
a
Perlakuan
4
65648.4
16412.1
1.19
4.12
7.85
B
730.06
a
Error
7
96652.8
Total
13
321919
Larnpiran 15.Anova Pengaruh Selenoproteinat dalam Ransum Sapi Perah Laktasi pada Glutation Susu (mgIL) Sumber Keragaman
db
JK
KT
'F
F.05
F.01
Perl.
Blok Perlakuan
2
1592.1
796.1
0.23
4.74
9.55
A
4
326851.2
81712.8
23.72
4.12
7.85
ABCE v s ~
1
321700.3
321700.3
93.40
5.59
ABE vs C
1
3732.3
3732.3
1.08
A vs BE
I
1415.8
1415.8
0.41
Error
8
27553.4
3444.2
Total
14
355996.8
Glutation
Signi fikasi a
B
278.5 304.5
12.25
c
337.0
a
5.59
12.25
D
672.6
b
5.59
12.25
E
305.8
a
a
Lampiran 16. Anova Pengaruh Selenoproteinat dalam Ransum Sapi Perah Laktasi pada SCC ( I0' sel/1111) Sumber Keragaman
db
Blok
2
Perlakuan
4
BDE vs AC
1
D vs BE
1
E vs B
1
C vs A
1
Error
8
Total
14
JK
KT
F
F.05
F.O1
Perl.
SCC
Signi fikasi
Lampiran 17. Anova Pengaruh Selenoproteinat dalam Ransum Sapi Perah Laktasi Pada T 3 darah (nM) Sumber Keragaman
db
JK
KT
F
F.05
F.O1
Perl.
T3
Signi fikasi
Blok
2
0.093
0.046
5.64
4.74
9.55
A
0.42
a
Perlakuan
4
1.052
0.263
32.00
4.12
7.85
B
1.07
b
A vs BCDE
1
1.048
1.048
127.58
5.59
12.25
C
1.07
b
BCD vs E
1
0.003
0.003
0.4 1
5.59
12.25
D
1.08
b
BC vs D
1
0.000
0.000
0.02
5.59
12.25
E
1.1 1
b
B vs C
1
0.000
0.000
0.00
5.59
12.25
Error
8
0.066
0.008
Total
14
Lampiran 18.Anova Pengaruh Selenoproteinat dalam Ransum Sapi Perah Laktasi pada IgG darah (nM) Sumber Keragaman
db
JK
KT
F
F.05
F.O1
Per1
IgG
Signifi kasi
Blok
2
1.797
0.898
2.67
4.74
9-55
A
1.443
a
Perlakuan
4
16.090
4.022
1 1.95
4.12
7-85
B
2.939
b
AC vs BDE
1
15.050
15.050
44.71
5.59
12.25
C
1.275
a
Bvs DE
1
0.969
0.969
2.88
5.59
12.25
E
3.567
b
E vs D
1
0.028
0.028
0.08
5.59
12.25
Error
8
2.693
0.336
Total
14
20.580
Larnpiran 19. Anova Pengaruh Selenoproteinat dalam Ransum Sapi Perah Laktasi pada Limfosit (% Leukosit) Sumber Keragaman
db
KT
JK
F
F.05
F.O1
Perl.
Limposit
Signi fikasi
Blok
2
17.2
8.6
0.85
4.74
9.55
A
55
a
Perlakuan
4
542.4
135.6
13.43
4.12
7.85
B
69
c
ACE vs BD
1
336.4
336.4
33.31
5.59
12.25
C
65
b
Avs CE
1
180.5
180.5
17.87
5.59
12.25
D
73
c
E vs C
1
1.5
1.5
0.15
5.59
12.25
E
64
b
B vs D
1
24
24
2.38
5.59
12.25
Error
8
80.8
10.1
Total
14
640.4
