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LAMPIRAN
54
LAMPIRAN 1 ROADMAP PENELITIAN Sumber kolagenase organisme perairan
Pilorik kaeka: Byun (2002) Tuna (Thunnus thynnus)
Organ dalam Kim (2002): filefish (Novodon modestrus) Park (2002): makerel (Scomber japanicus)
Hepatopankreas Aoki (2003) : Udang (Pandalus eolus) Rudenskaya (2004) : Kepiting raja (Paralithodes camtschaticus)
Usus
Jaringan Kim (1991): (Novodon modestrus)
Yuniarti (2008)
Bandeng (Chanos chanos, forskal)
Pilorik kaeka
Kelenjar pencernaan Simpson (2000): Kepiting, lobster, udang
Hati
Purifikasi dan karakterisasi Kolagenase
KOLAGENASE
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Lampiran 2 Tabel Konversi dari g (gravity) ke rpm (revolutions per minute) Konversi dari g ke rpm berdasarkan rumus:
Dimana: g = gravity atau gaya sentrifugal relatif (RCF = Relative Centrifugal Force) R = jari-jari rotor (cm) S = kecepatan putaran dalam rpm (revolutions per minute)
Sumber: Pierce Biotechnology, Inc. 2005. Technical Resource Convert Between Times Gravity (×G) And Centrifuge Rotor Speed (Rpm). Rockford, USA: Pierce Biotechnology, Inc. www.piercenet.com.
56
Lampiran 3
57
Lampiran 4 Organ dalam ikan bandeng (Chanos chanos, Forskal)
Hati
Usus Pilorik kaeka
58
Lampiran 5 Hasil perhitungan elekroforesis
4 5 6 7 8
Marker Phosphorilase b Albumin Oval bumin Carbonic anhidrase tripsin inhibitor α-laktalbumin Band 1 Band 2
Batas atas (cm) 0,7 1,5 2,3
BM 97.000 66.000 45.000
Rf 0,14 0,3 0,46
Log BM 4,986772 4,819544 4,653213
5 5 5 5 5
3,1 3,4 4,0 3,0 4,1
30.000 20.100 14.400 27.612,14 14.636,59
0,62 0,68 0,80 0,60 0,82
4,477121 4,303196 4,158362 4,44110 4,16544
Kurva regresi linear berat molekul marker dan Rf 6 5 4 Log BM
No 1 2 3
Batas bawah (cm) 5 5 5
y = -1,253x + 5,1929 2
3
R = 0,9828
2 1 0 0
0,2
0,4
0,6 Rf
0,8
1
