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Atlas, R.M. dan M. Bartha. 1987. Microbiai Ecology, Fundamentals and Applications.The Benjamin Publishing Company Inc. California. p.34--41 Baker, A.J.M. 1987. Accumulaters and Exluders Strategies in the Response of Plant to Heavy Metals. Journal of Plant Nutrition. 3(3) :343-654 Bartelmus, P. 1986. Environment and Development. Allen & Unwin. Boston. P. 174--176 Bosse, U. dan W.E. Peter . 1997. Activity and Distribution of Methane-Oxidizing Bacteria in Flooded Rice Soil Microcosms and in Rice Plants p r y z a sativa). Applied and Environmental Microbiology. American Society for Microbiology. 63(4):24 -29 Brown, P.H., R.M. Welch and E.F.Cary. 1987. Nickel : A Micronutrient Essential for Higher Plants. Plant Physiology. 5:801-803 Botts, A. J.. B.Moore and W. L. Goodfellow. 1989. Toxicity Identification Evaluation. Toxicity Reduction Evaluation Protocol for Municipal Wastewater Treatment Plants. U.S. Environmental Protection Agency, Ohio. 62 : 4 1 4 3 [BPPTj Badan Pengkajian dan Penerapan Teknologi, Direktorat Pengembangan Lahan dan Mitigasi Bencana. 1997. Manajemen Limbah Cair Industri. Jakarta. haf. 6-1 7 Budiharjo, E. 1984. Sejumlah Masalah Pemukiman Kota. Alumni. Bandung. hal. 21-29 Case, D. 1994. Water Garden Plants. Redwood Books. Trowbridge, Wilthire. p. 1 4 0 Chaerulwan, U. den Krismono. 1998. Beberapa Aspek Limno-Biologi dan Perikanan di Danau Tondano, Sulawesi Utara. Jurnal Perikanan Indonesia 4 (4): 1 - 3 0

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LAMPIRAN

Tabel lapiran 1 : HASIL PENGUKURAN KUALITAS LIMBAH SETELAH MELALU! PROSES BlOREMEDlASl

7 / ~erlaruan s u h u AOBO

) 28.00

AOBI

28.00

P

e

u

b

~ e k e r u h a n~ d tTerousp . ( JTU) ( mgll)

( O c

a

h p~

DHL-

pmhoslcm)

126.62

120.56

6.70

127.83

120.00

6.70

118.20

6.70

114.70

6.70

73.88

6.90

108.50

70.00

6.90

108.20

58.86

6.90

107.70

45.00

6.90

107.00

160.50

1

ADB2 AOB3

28.00

1

I

28.00

127.00

i

126.20

A1BO

27.00

64.15

A161

27.00

63.20

I

1

154.66

A260

26.25

58.54

53.10

7.00

96.80

A251

26.25

48.03

50.10

7.00

96.20

A282

26.25

39.88

45.00

7.00

95.80

A2B3

25.25

37.24

40.00

7.00

95.30

A380

25.25

34.54

41.14

7.20

94.90

A3B 1

25.25

28.06

I

40.05

7.20

94.55

A382

25.25

25.85

I

39.16

7.20

94.50

A3B3

24.25

23.52

38.00

7.20

94.20

Keterangan .

A = Komposisi tanaman air. 3 = Pengenceran lrmbah

1

1

Sambungan tabel lampiran 1

Keterangan :

A. = Komposisi tanaman air B = Pengenceran limbah

Sambungan tabel lampiran 1 P

Periakuan

~e"

Ci-

AOBO

( mg/J ) 0.84

( mg/! ) 34.29

e

u

b

S O . ' ( mgll ) 38.52

Keterangan : A = Komposisi tanaman air B = Pengenceran limbah

a

h

NH3 ( mg/l ) 15.44

NO -

(mwfl

26.40

Sarnbungan tabel larnpiran 1 Pertakuan AOBO

NO2( mgll ) 0.38

caZ'

P

( mgll )

32.24

e u b a h ~ n " E. coli ( s e W 1 0 0 ml) ( mgll )

0.32

Keterangan : A = Komposisi tanaman air B = Pengenceran limbah

9.6 x

lo4

Coliform (seU'tOO mf ) 0.6~10~

Tabel Lampiran 2 : HASlL ANALISfS SlSlDK RAGAM PENGARUH PERLAKUAN TERHADAP PENURUNAN SUHU

***

BY

Y-l Fl F2

ANALYSIS OF VARIANCE

SUHU ('c FAKTOR FAKTOR

Sum of Square

*"

) A ( KOMPOSISI TANAMAN AIR') B ( PENGENCERAN LIMBAH )

DF

Mean Square

F

6

8.938

35.750

.OOO

F1

3

'17.188

68.750

,000

F2

3

.687

2.749

,000

Source of variance Main Effects

2 -Way Interactions

F1 - F2 Expla~ned Residual Total

48 Cases were processed 0 Case ( .O PCT ) were missing

Siginificant of F

Tabel Lampiran 3 : HASIL ANALISIS SlSlDK RAGAM PENGARUH PERLAKUAN TERHADAP PENURUNAN KEKERUHAN

*tt

BY

Y2 F1

F2

ANALYSIS O'F VAR!ANCE

'**

KEKERUHAN ( JTU ) FAKTOR A ( KOMPOSlSl TANAMAN AIR ) FAKTOR B ( PENGENCERAN LIMBAH )

Mean

Siginificant of F

Source of variance

Sum of Square

DF

Square

F

Main Effects

79338.343

6

13218.724

20983.t39

.OOO

F1

79317.779

3

26439.260

420337.99

,000

F2

,564

3

188

.31

,664

2 -Way interactions

F1 - F2 Explained Residual Total


Tabel Lampiran 4 : HASlL ANALISIS SlSlDK RAGAM PENGARUH PERLAKUANTERHADAPPENURUNANPADATAN TERSUSPENSl

c**

BY

Y3 F1 F2


"'

PADATAN TERSUSPENSI ( rng/l) FAKTOR A ( KOMPOSlSl TANAMAN AIR ) FAKTOR B ( PENGENCERAN LIMBAH )

Source of variance

Sum of Square

DF

Mean Square

F

Main Effects

43363.721

6

7227.286

234985.96

000

F1

43057.276

3

14352.425

466650.69

.OOO

F2

306.445

3

102.448

3321.22

.OOO

2

- Way

Interactions

F1 - F2

Explained Residual Total

48 Cases were processed 0 Case ( .O P C T ) were missing

Siginificant of F

Tabel Lampiran 5 : HASlL ANALISIS SlSlDK RAGAM PENGARUH PERLAKUAN TERHADAP PENURUNAN pH

***

BY

Y4 F1 F2


pH FAKTOR FAKTOR

Sum of Square

Mean Square

6

,219

Fl

3

,425

85.00

000

F2

3

,014

2.79

000

Main Effects

-

A ( KOMPOSlSl TANAMAN AIR ) B ( PENGENCERAN LIMBAH )

DF

Source of variance

2

'**

Way tnteract~ons

F1

-

F2



F

43.89

Siginificant of F ,000

Tabel Lampiran 6 : HASlL ANALISIS SlSlDK RAGAM PENGARUH PERLAKUAN TERHADAP PENURUNAN DHL

***

BY

Y5 F1

F2

ANALYSIS' OF VARIANCE

"*

DHL ( g~rnhoslcm) FAKTOR A ( KOMPOSlSl TANAMAN AIR ) FAKTOR B ( PENGENCERAN LIMBAH )

Source of variance

Sum of Square

Mean Square

F

Main Effects

30650.300 6

51 08.383

39662.64

.OOO

F1

306257.325 3

10209.108

79625.83

.OOO

F2

22.975

3

7.658

59.46

,000

54.420

9

6.047

46.95

.OOO

6.047

46.95

.OOO

DF

Siginificant of F

2 - W a y Interactions F1 -F2

Explained

30704.720 15

Residual

4.121

32

.I29

Total

30708.841

47

653.380


Tabel Lampiran 7 : HASIL ANALISIS SISIDK RAGAM PENGARUH PERLAKUAN TERHADAP PENURUNAN DO

***

BY

Y6 F1 F2


DO ( mgll ) FAKTOR A FAKTOR 3

***

( KOMPOSlSl TANAMAN AIR ) ( PENGENCERAN LIMBAH )

DF

Mean Square

6

2.053

2451.02

.OOO

F1

3

3.799

4536.51

.OOO

F2

3

,306

Source of variance

Sum of Square

Main Effects

2 -Way Interactions



F

Siginificant of F

365.52

000

Tabel Lampiran 8 : HASIL ANALISIS SlDlK RAGAM PENGARUH PERLAKUAN TERHADAP PENURUNAN BOD ***

Y7 BY F1 F2

Source of variance


***

BOD ( mg/l ) FAKTOR A ( KOMPOSlSl TANAMAN AIR ) FAKTOR B ( PENGENCERAN LIMBAH )

Sum of Square

Main Effects

Mean Square 4331.953

F

Siginificant of F

38579.62

.OOO

F1

8650.571 77399.930

.OOO

F2

13.335

119.3TO

,000

2 - Way Interactions



Tabel Lampiran 9 : HASlL ANALlSlS SlDIK RAGAM PENGARUH PERLAKUAN TERHADAP PENURUNAN COD ***

Y8 BY F1 F2


*"

COD ( mgli ) FAKTOR A (KOMPOSISITANAMANAIR) FAKTOR B ( PENGENCERAN LIMBAH )

Source of variance

Sum of Square

Mean Square

F

Main Effects

163963.648 6

27327.275

6831 818.75 .DO0

F1

163698.843 3

54566.281

13641500.00 .OOO

F2

264.805

3

88.268

22067.00

,000

723.291

9

80.368

20092.00

,000

Explained

164686

15

10979.129

2744782.25 ,000

Residual

0.1 15

32

,004

Total

164687.054 47

2

- Way

DF

Siginificant of F

Interactions

F1 - F2


3503.980

Tabel Lampiran 10 : HASlL ANALISIS SlDlK RAGAM PENGARUH PERLAKUAN TERHADAP PENURUNAN Con **l

BY

Y9 F1 F2


***

C O z ( mgll ) FAKTOR A ( KOMPOSISI TANAMAN AIR ) FAKTOR 3 ( PENGENCERAN LIMBAH )

Source of variance

Sum of Square

Mean Square

Main Effects

3678.417

613.069

180535.79

.OOO

F1

3613.744

1204.581 354723.34

- .OOO

F2

64.673

21.558

2 - Way Interactions F1 - F2 Explained Residual Total


F

Siginificant of F

6348.23

.OOO

Tabel Lampiran It : HASIL ANALISIS SlDlK RAGAM PENGARUH PERLAKUAN TERHADAP PENURUNAN ORTOFOSFAT f.r*

BY

Y10 Fl F2

Source of variance

ANALYSIS OF VARIANCE *-

ORTOFOSFAT ( mgll ) FAKTOR A ( KOMPOSlSl TANAMAN AIR ) FAKTOR B ( PENGENCERAN LIMBAH )

Sum of Square

Mean Square

F

Siginificant of F

422 1.02

.000

F1

7997.49

.000

i= 2

444.55

.ooo

Main Effects


F1 - F2 Explained Residual

Total


Tabe[ Lampiran 12 : HASlL ANALrSlS SlDlK RAGAM PENGARUH PERLAKUAN TERHADAP PENURUNAN ~ e ' ***

Y11 BY F l

F2


"*

~ e ( ~mgll ' ) FAKTOR A ( KOMPOSlSl TANAMAN AIR.) FAKTOR B ( PENGENCERAN LIMBAH )

Sum of Square

Mean

Square

6

,414

F1

3

,814

3829.74

.OOO

F2

3

.Oq4

63.65

-000

Main Effects

2 - Way Interactions F l - F2

Explained Residual

Total


F

Siginificant of F

DF

Source of variance

1946.79

,000

Tabel Lampiran 13 : HASIL ANALEIS SlDlK RAGAM PENGARUH PERLAKUAN TERHADAP PENURUNAN CI***

BY

Y12 F1 F2


CI- ( mg/l ) FAKTOR A FAKTOR B

**"

( KOMPOSISI TANAMAN AIR ) ( PENGENCERAN LIMBAH )

Source of variance

Sum of Square

DF

Mean Square

Main Effects

4130.220

6

688.370

F1

4128.307

3

1376.702

821 553.71

.OOO

F2

1.912

3

,637

380.52

-000

2

- Way

Interactions

F1 - F2 Explained

Residual Total

48 Cases were processed 0 Case ( .O,PCT ) were missing

F

Siginificant of F

410967.12

.OOO

Tabel Lampiran 14 : HASlL ANALISIS SlDtK RAGAM PENGARUH PERLAKUAN TERHADAP PENURUNAN So,+'' -*

BY

YI 3 F1 F2

ANALYSIS OF VARIANCE "'*

SO^'-( mg11 ) FAKTOR A ( KOMPOSlSl TANAMAN AIR ) FAKTOR B ( PENGENCERAN LIMBAH )

Sum of Source of variance

Square

DF

Mean Square

Main Effects

6089.296

6

1014.883

762353.20

,000

F1

6083.973

3

2027.991

1523373.6

,000

F2

5.323

3

1.774

1332.78

.OOO


F1 - F2



F

Siginificant of F

Tabel Larnpiran 15 : HASlL ANALISIS SlDlK RAGAM PENGARUH PERLAKUAN TERHADAP PENURUNAN NH3 ***

BY

Y14 F1 F2


**"

NU3( mgfl ) FAKTOR A ( KOMPOSlSl TANAMAN AIR.) FAKTOR B ( PENGENCERAN LIMBAH )

S u m of DF

Mean Square

6

119.468

292575.37

.OOO

F1

3

232.672

569807.92

.OOO

F2

3

6.265

15342.82

,000

Source of variance

Square

Main Effects




F

Siginificant of F

Tabel Lampiran 16 : HASlL ANALISIS SlDlK RAGAM PENGARUH PERLAKUAN TERHADAP PENURUNAN NO< ***

BY

Y15 F1 F2


***

NOs ( mgll ) FAKTOR A ( KOMPOSISI TANAMAN AIR) FAKTOR B ( PENGENCERAN LIMBAH )

Source of variance

Sum of Square

DF

Mean Square

F

Main Effects

2525.638

6

420.940

706472.7 2

F1

2523.017

3

841.006

1411478.00 ,000

F2

2.621

3

,874

1466.20




Siginificant of F .OOO

,000

Tabel Lampiran 17 : HASlL ANALISIS SlDlK RAGAM PENGARUH PERLAKUAN TERHADAP PENURUNAN NOz-

BY

Y 16 F1 F2

N02- ( rngll ) FAKTOR A ( KOMPOSlSl TANAMAN AIR ) FAKTOR 3 ( PENGENCERAN LIMBAH )

Sum of Square

DF

Mean Square

F

6

. I26

830.64

,000

F1

3

.251

1650.21

.OOO

F2

3

.002

1 1.08

,000


2 -Way Interactions F1I - F2



Siginificant of F

Tabel Lampiran 18 : HASlL ANALISIS SIDIK RAGAM PENGARUH PERLAKUAN TERHAOAP PENURUNAN ca2' ttt

BY

Y17 F1 F2

ANALYSlS OF VARIANCE

*"

ca2'( mgll ) FAKTOR A ( KOMPOSISI TANAMAN AIR ) FAKTOR B ( PENGENCERAN LIMBAH )

S u m of Source of variance

Square

DF

Mean Square

Main Effects

4608.066

6

768.01 1

134443.94

.OOO

F1

4606 336

3

,577

268786.93

,000

F2

1.730

3

.577

1C3.96

,000

.645

9

,072

12.55

.OOO

Explained

4608.71 1

15

307.247

53785.11

.OOO

Residual

,183

32

.006

Total

4608.894

47

98.062

F

Siginificant of F

2 -Way Interactions F1 - F 2


Tabel Lampiran 19 : HASIL ANALISIS SlDIK RAGAM PENGARUH PERLAKUAN TERHADAP PENURUNAN ~ n * ' *I,+

BY

Y18 F1 F2

ANALYSIS OF VARIANCE ***

~ n ' ' ( mgll ) FAKTOR A ( KOMPOSlSl TANAMAN AIR ) FAKTOR 6 ( PENGENCERAN LIMBAH )

Sum of Square

DF

Mean Square

F

6

,073

400.01

.OOO

F1

3

144

784.10

.OOO

F2

3

,003

15.92

.OOO


2 - Way Interactions F ? - F2



Siginificant of F

Tabel Lampiran 20 : HASlL ANALISIS SlDlK RAGAM PENGARUH PERLAKUAN TERHADAP PENURUNAN Coliform ***

BY

Y19 F1 F2


*-

Coliform ( sell1 00 ml ) FAKTOR A ( KOMPOSlSl TANAMAN AIR ) FAKTOR B ( PENGENCERAN LIMBAH )

Source of variance

Sum of Square

DF

Mean Square

F

Main Effects

510.275

6

85.046

92.95

F1

493.979

3

164.660

3 79.96

F2

16.296

3

5.432

5.94

47

11.310

2

- Way

Interactions

F1 - F2

2.000

Explained

502.275

Residual

29.280

Total

531.555


Sigin~ficant of F 000 .OOO 000

Tabet Larnpiwn 21 : HASIL ANALISIS SlDlK R A ~ A M PENGARUH PERLAKUAN TERHADAP PENURUNAN E.coli ***

BY

Y20 F1 F2


E.coli ( se1/100 ml ) FAKTOR

FAKTOR

S u m of Square

( KOMPOSlSl TANAMAN AIR B ( PENGENCERAN LiMBAH )

A

Mean

Siginificant of F

DF

Square

F

6

1 83

12.90

.OOO

FI

3

,321

22.65

.OOO

F2

3

,045

3.15

,038


2

***

- Way F1

Interactions

- F2



Tabel lampiran 22 : BAKU MUTU LIMBAH RUMAH TANGGA ( SESUAl SK MENEG KLH. NO.KEP.3IMENKLHII1/1991 DAN PP N0.20 TAHUN 1990 )

Xis

P e u b a h

Satuan

t

2

Kadar yang diperbofehkan

3

4

FlSlK

1

Suhu

2 3

Kekeruhan Padatan tersuspensi

mg/l

4

Zat padat terlarut

mg/l

5

Padatan ferendap

mg/l

O c

JTU

KIMIA 1

PH Besi terlarut

mgfl

3

Mangan terlarut

mgll

4

Barium

mg/i

5

Tembaga

mg/l

6

Seng

mg/l

7

Kadmium

rngll

8

Nikef

mg/l

9

Perak

mg/l

2

10

Raksa

mg/l

11

Ammonia

mg/l

12

Klor

mg/l

13

DO

mgA

74

80D5

mg/l

15

COD

mgfl

16

DHL

17

Con --

prnhos ,

mgll

18

Ortofosfat

I

mgll

8 - 12

1

mgfi

8- 12

mg/)

10

mgll

0.06

19

Sulfat

20

Nitrat

21

Nitrit

22

Kalsium

mg/l

5-10

23

Sbnida

m g/1

0.02

mgil

0.01

mg{l

1

24

Fenol

25

Minyak dan iemak

i I I

1

MlKROBlOLOGl ?

Coliform

se1l100 mi

20.000

2

E.coli

sell100 rnl

100

i

Tabel Lampiran 23 :

1. Jumlah

HASlL PENGAMATAN PADA UJI LC50 DAN PEMANTAUAN BOBOT BADAN IKAN

lkan yang Hidup Setelah Pemberian Limbah

2. Bobot Badan lkan Setelah Pemberian Limbah Konsentrasi C ikan Bobot badan ikan ( dalam gram ) limbah ( O h ) (ekor) Seri ke : 0 jam ] 24 jam 1 48 jam 72 jam 1 10 10

0

3 Raterat.

25

10 10

, , J r i

1

3

-1 1

i

1 !

10

_

_

~

2 3 kRats:r.ata

~ -].-

35 32 34 33.7

36 33 , 34 34.3 1 35 33 I 33 35 . 35 34.3 34.3 36 36 I 34 34 33 33

34 3 4 ;;3

!Ratzzty

l l o 1 Rata-rata ~

-

35

35 32 33 33.3

34 32 33 33.0

96 jam 35 32 34 33.7

33.3 32 30 35 32.3 32.5

29.2 29 30 29.5 29.5 27 27 20.5

27.2 29 24 29.5 27.5 24 20.5 20.5

27 26.5

.x4-9.~267-~248L.~

DAFTAR PUSTAKA. Bartelmus, P Environment and Development. Allen & Unwin. Boston

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