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Lampiran 1 a. Komposisi vitamin pakan percobaan.
Vitamin
mg per kg pakan
Vitamin B, Vitamin B, Vitamin B, Vitamin B,, Asam pantotenat Inositol Biotin Asam folat Asam para amino benzoat Kolin Niasin Vitamin A Vitamin E Vitamin K Jauncey dan Ross ( 1 982) Lampiran 1 b. Komposisi mineral pakan percobaan
Mineral CaHPO, 2 H,O MgSO, 7 H 2 0 NaC l Kc1 FeSO, 7 H,O ZnSO, 7 H 2 0 MnSO, 7 H 2 0 CuSO, 4 H,O CoSO, 7 H 2 0 CaIO, 6 H,O CrCI, 6 H 2 0 Jauncey dan Ross ( 1982 1
g per kg pakan
Lampiran 2. Distribusi diameter oosit pada setiap stadium.
Kelas diameter (mm)
Total
Stadium 2
3
4
Total
1 0 0 .OO
100 - 0 0
Keterancran = a = frekuensi kelas ukuran oosit b = prosentase c = prosentase dalam baris d = prosentase dalam lajur
100 . O O
100.00
400.00
Lampiran 3 . Tingkat kernatangan gonad (TKG), indeks gonad vitamin C ovarium. dan distribusi oosit ikan nila.
No
TKG
Rataan
IGS
0.157
vitamin C (ug/g) < 0.40 (mm)
74.33
100
somatik (lGS=%),
diameter oosit 0.41-0.80
(mm)
0
0.81-1.80
1.81-2.20
(mm)
(mm
0
0
Rataan
Rataan
0.373
115.44
74.52
25.48
0
0
Rataan
3.071
121.95
15.51
15.25
Rat aan
1.592
108.25
45.66
47 - 6 6
Keteranean
=
*
contoh tidak cukup
8.60
6.67
60.64
0
Lampiran 4. Distribc~siindeks gonad souiatik (IGS) pada t i n s l a t kematangan g o n a d (TKC;)
TKG
Kelas IGS (%)
I
II
II I
'Total
Iv
v
Total
10.00
23.00
Keterangan : a
=
b
=
frekuensi IGS prosentase c = prosentase datam baris d = prosentase dalam lajur
23.00
35.00
9.00
100.00
131
Lampiran 5 a. Kro~natografihasil analisis vitamin C telur. hati. dan ovariuln ( V ) .
Ovarium
Lampiran 5 b. Kromatografi standar vitamin C (V)
Hati
Lampiran 6 . Distribusi kandungan vitamin C ovarium pada setiap tingkat kematangan gonad (TKG).
TKG
Kelas kandungan vitamin C ovarium
(uds)
I
II
111
Total
Iv
v
Total
8.00
23-00
23 -00
3.00
Keteranvan : a = frekuensi kandungan vitamin C pada TKG b = prosentase c = prosentase pada baris d = prosentase pada lajur
9.00
98 - 0 0
Kandungan vitamin C hati (ug/g) ikan nila yang menerin~apakan dengan suplernentasi berbagai tipe vitamin C setelah masa pelneliharaan 42 hari.
Lampiran 7.a
S u m b e r vitamin
Ulangan 1
2
Kataan vitarnin C (uds)
3
Lampiran 8 b. Kandungan vitamin C usus (ug/g) ikan nila vang menerima pakan dengan suplementasi berbagai tipe vitaminC setelah niasa percobaan 4 2 hari (uji "ketersediaan biologi").
S u m b e r vitamin
Ulangan 1
2
Keterangan : kontrol (tanpa vitamin C) askorbil-2-fosfat magnesium (APM) = askorbil-2-polipfosfat kalsium dilapisi sitikon D = asam askorbat dilapisi lemak ranaman E = asam askorbat (vitamin C) A
=
B C
=
Rataan vitamin C 3
Cugk 1
Lampiran 8 a. Analisis ragam pengaruh suplementasi beberapa tipe vitamin C terhadap retensi vitamin C hati ikan ni1a.
Sumber keragaman
Db
PerIakuan AVSBCDE BC Vs DE BVSC DVsE Galat
4 1 1 1 1 10
7546.4219 6612 -6902 543.6494 288.5653 288.5653 1060.1942
Total
14
8606.6161
6612 -6902 62.37'*' 543.6494 5.13" 288.5653 22.72 101.5170 00.95 106.0194
10.04
Keterangan : *'
nyata pada taraf (P c 0.05) sangat nyata pada taraf (P < 0.01)
*&>
A
= Kana-01
BC = Kelompok askorbil fosfat
VS
BCDE
VS
DE
B = Askorbil-2-mono fosfat magnesium
VS
D
VS
=
Asam askorbat dilapisi lemak tanaman
E
=
Kelompok vitamin C
= Kelompok asam askorbat
= Asam askorbat
4-96
Lampiran 8 b Anatisis ragarn pengaruh beberapa tipe vitamin C usus ikan nila.
Db
Surnber keragaman
JK
1 1 10
2056.4610 6481.3067 2893.5675
Total
14
75147.1098
1 1
"
"'
F h,,
KT
Perlakuan A VS BCDE BC Vs DE I3 VS C D VS E Galat
4
vitamin C terhadap retensi
72253.5423 45634 -9712 18080.8033
45634.9712 18080.8033 2056.6410 6481.3067 289.3567
F,a~, (1%)
(5%)
157.71'*' 10.04 62.48*+)
4.96
7.10"' 22. 26*"
nyata pada taraf (P < 0. 0 5 ) sangat nyata pada taraf (P < 0. 0 I.)
A = Kontrol
VS
BCDE
=
BC= Kelompok Askorbil fosfat
VS
DE
= Kelompok asam askorbat
B = Askorbil 2-mono fosfat magnesium
VS
C
D = Asam askorbat dilapisi iemak tanaman
VS
=
Krlornpok vitamin C
Askorbil2-polifosfat kalsium
Lampiran 9 a.
Rataan prosentase (%) vitamin C hilang selarna lnasa perendaman (3, 6 , 12 rnenit) dari pakan yang ~nengandung beberapa tipe vitamin C.
% vitamin C hilang setelah perendaman menit ke-
Sumber vitamin
Lampiran
3
6
12
9 b. Rataan prosentase (%) vitamin C hilang selama proses pernbuatan pakan berupa pelet.
% hilang
Sumher vitamin
Keterangan : = askorbit-2-fosfat magnesium C = askorbil-2-polifosfat kalsium dilapisi silikon D = asam aikorbat dilapisi lernak tanaman E = asaln askorbat (vitamin C )
3
Lampiran 10 a. Perkembangan indeks gonad somatik (IGS) dan tingkat kematangan gonad (TKG) ikan nila betina pada setiap perlakuan.
Dosis APM (mag
IGS dan TKG pengamatan hari ke 0
18
IGS TKG IGS 0
Rataan 750
36
TKG
0.19 I 0.2511 0.20 I 0.15 I 0.05 I 0.24 I 0.16 I 0.15 I 0.18 I 0.03 I
0.69 I1 0.29 I1 0.29 I1 0.55 I1 0.89 I11 0.77 I1 0.45 I1 0.67 I1
0.16
0.545
0.38
*
0.40
I1
I1
0.76 I1 0.25 I1 0.22 I 0.82 I11 0.42 I1 0.22 I 1.61 I11 0.34 I1 0.549
1500
0.63
*
0.41 0.62 0.44
*
0.507
1.08 0.82 0.62 0.79 0.80 0.53 0.38
I1 I1 I1 I1
IGS
72
TKG
IGS
TKG
I11 I11 I1 I11 111 I1 I1
1.66 I11 2.39 1.31 I11 2.53 0.74 I1 2.02 1-05 111 1.92 1.13 111 1.65 * 1.71 1-01 I11 * * 0.74 I1 1.02 1-08 I11 0.82 I11 1.18 0.25 I1 1.31 I11 1.55
0.42 0.63 1.21 0.68 1.27 1.37 0.43 0.38 1-07
*
1.086 I1 2.01 111.66 I11 2.03 11 1.27 I11 1.36 I11 1.62 I1 0.52 I1 * I11 1.37 1.02
0.828
0.34 I1 0.23 I1 1-30 I11 0.60 I1
Rataan
54
TKG
0.705
rc
Rataan
IGS
0.46 0.48 0.57 1.14 0.69 1.86 0.72 1.57 0.62 0.85 0.896
*
1.91 2.67 1.38 2.02 1.42
* 1.49 0.99 1.07 1.618
I11 111 I11
1.807 I11 2-61 1112.96 I11 3.04 I11 2.56 I11 1.67 I11 1.66 I1 1.72 1.52 111 1.68 11 *
1.428 I1 I1 I1 I11 I1 I11 I1 I11 I1 I11
IV IV 111 I11 I11 I11
IV IV IV IV 111 111 I11 111 111
2.157 3.06 3.17 2.32 3.15 2.56 2.04 1.84 I11 2.33 111 1.93 111 2.43
I11 IV I11 I11 I11
IV IV IV IV IV I11 I11 IV I11 IV
2 -480
Rataan
0.523
1.025
I 1 1.12 I 1 2.21
Rataan
0.413
0.696
2 -751
1.736
1.586
III IV
2.38 3.77
2.507
IV IV
Lampiran I0 b. Daftar sidik ragam indeks gonad somatik ikan nila pada setiap periode pengamatan.
Hari k e IS -
-
-
-
-
Surnber keragarnan
Db
Dosis Linier Kuadratik
4
1 1
JK
KT
0.1485558 0.0792066 0.0792066 0.0565355 0.0565355
Galat
40
3.9570086
Total
44
4.1055644
0.80 0.57
0.3762 0.4541
0.14 0.92
0.7-071 0.3435
0.0989252
Hari k e 3 6
Surnber keragaman Dosis Linier Kuadratik
Total
Db
4 1 1
44
0.4835814 0.0297409 0.1907609
8.7881200
0.0297409 0.1907689
Hari ke 5 4
Sumber keragarnan
Db
JK
Dosis Linier Kuadratik
4 1 1
Galat
39
15.4691986
Total
43
17.1746977
1.7054991 1.1628100 0.4962625
KT
1.1628100 0.4962625
FI,,,
2.93 1.25
Pr > F
0.0948 0.2702
0.39664612
Hari ke 72 --
Sumber Keragaman
Db
Dosis Linier Kuadratik
4 1
Total
-
JK
1
5.3539422 3.8192400 1.3495365
44.;
22.4079200
3 -8192400 1.3495365
Keteranean : * ) nyata pada taraf (P < 0.0 5 )
**) sangat nyata pada taraf (P
i
o .o 1 )
8.96.-' 0.43
0.0047 0-5139
142
Lampiran I 1 a. Indeks hepar somatik (IWS) induk ikan nila pada setiap perlakuan.
Dosis APM ( m d k g pakan) 0
Rataan 750
IHS pengarnatan hari ke
o
18
36
54
72
1.07
0.96 0.93 1.26 0.98 1.14 0.97 0.99
0.96 1.04 1.21 0.99 0.98
0.99 0.99 1.02 1-08 1.12 1.02
0.28 0.42 0.66 0.43 0.26 0.30 0.38 0.37 0.28 0.39
0.97 1.08 0.96 0.92 1.28 1.19 0.98 1.12
0.377
t
*
*
1.26 0.82
1.06 0.98 0.97 0.95
1.04 1.06 1.08
1.063
1.034
1.015
1.044
1.39
1.35 1.30 1.33 1.53 1.32 1.36 1.28 0.99 1.31
1.26 1.35
1.23 1.15 1.35 1.32 1.25 1.21 1.21 1.37 1.32
*
1.13 0.88 1.16 1.12 1.05 0.86 1.07 0.87
*
*
1.37 1.27 1.32 1.31 1.13
*
1.21 1.02
1~
Rataan
1.058
1.307
15 0 0
1.16
1.21
1.31 1.29 1.25 1.46 1.39 1.42 1.32 1.28 1.31 1.32
1.49 1.42 1.21
1.27 1.29 1.05 1.17 1.23 1.19 1.45 1.24 1.37 1.24
1.116
1.335
1.320
1.250
*
0.96 1.06 1.12
*
1..14 0.96
1.32
Rataan
1.248 x
1.32
1.29 1.24 1.27 1.32 x
1.267
Rataan
Rataan K e t e r a n ~ a n:
'
*
= jantan '
1.066
1.376
1.325
1.264
1.113
1.337
1.284
1.234
Lampiran I I b. Daftar sidik ragam indeks hepar somatik ikan nila pada setiap periode pengamatan. Hari ke 18
Sumber keragaman
Db
Dosis Linier Kuadratik
4 1 1
0.0356223 0.0151474 0.0001362
0.01514741 0.00013627
GaIat
40
0.9038863
0.02259716
Total
44
0.9394977
JK
0.67 0.01
0.4172 0.9385
F,,
Pr > F
Hari k e 36
Sumber keragaman
Db
Dosis Linier Kuadratik
4 1 1
JK
0.7077688 0.2901344 0.3702293
KT
0.2901344 0.3702293
Keteranean :
*)
nyata pada taraf (P < 0.05)
* *) sangat nyata pada taraf (P < 0.0 1)
35.97"' 45.90"'
0.0001 0-0001
Hari ke 54
Sumber keragaman
Db
Dosis Linier Kuadratik
4 1 1
JK
0.62502500 0.23818778 0 -38411034
0.23818778 0.38411034 0.01133846
Galat
39
0.44220000
Total
43
1.06722500
Hari ke
KT
21.01' 33.08.-'
0.0001 0.0001
72
JK
Sumber keragaman
Db
Dosis Linier Kuadratik
4 1 1
0.3585560 0.0768574 0.2754024
43
0.7041726
Total
Keteranean
KT
0.0768574 0.2754024
:
*) **)
nyata bada taraf (P c CI - 0 5 ) sangat nyata pada taraf (P < 0 - 01)
8.67*' 31.08*"
0.0054 0 0~0~0~1
.
Lampiran 12 a . Kandungan estradiol serum (ndml) pada setiap perlakuan selatna 7 2 liari percobaan.
Dosis APM ( m d k g ~ a k a )n
Rataan
Rataan
Rataan
Periode pengamatan (hari) 0
18
36
54
6.05
9.00
1.12
3.67
3.25
7 -95
htaan
3.41
Rataan Keteranean :
* = jantan '
r e =
contoh darah tidak cukup
6.64
8.69
7.57
Lampiran 13 b. DaRar sidik ragam kandungan estradiol serum setiap perlakuan selama 72 hari percobaan. Hari ke 18 Sunber keragaman
Db
KT
JK
F ,
Pr > F
Dosis Linier Kuadratik
Total
Hari ke
41
36
Surnber keragaman Dosis Linier Kuadratik
Total
125.59540465
Db
JK
4 1 1
24.8873022 20.4966344 3.3581341
41
295.7999880
KT
20.4966944 3.3581341
F,t
Pr > F
3.03 0.50
0.0896 0.4854
Hari ke 5 4
Sumber keragaman Dosis Linier Kuadratik Galat
34
Total
38 101.74640000
71.38357500
2.03953071
Hari k e 7 2
Sumber keragaman
Db
IK
Dosis linier Kuadratik Galat
4 0 116.294048
Total :
44
2.90735
142 -338458
Keteranean : *) nyata pada taraf (P < 0.05)
**) sangat nyata pada taraf (P < 0.01)
Larnpiran 13 a. Kandungan kolesterol ovarium (mg/g) pada setiap perlakuan selama 72 hari percobaan.
Dosis APM (mg/kg ~ a k a n
Rataan
Periode pengamatan (hari) 0
18
36
54
72
4.867
9.584
8.396
8.446
Rataan
3.940
7.485
5.821
5.727
Rataan
3.621
6.637
5.745
3.12
5.357
Rataan
3 -781
6.144
5.398
5.307
Rataan
3.167
6.217
4.978
5.348
Lampiran 13 b. Daftar sidik ragam kandungan kolesterol ovarium seiama 72 hari percobaan . Hari k e 18
JK
KT
Sumber keragarnan
Db
Dosis Linier Kuadratik
4 1 1
11.0613828 9.2238300 0.6911520
9.22383000 0.69115204
Gal at
30
20.4011714
0.68003905
Total
34
31,4625542
Y
= 4.9501
+
Fhit
Pr> F
13.56**' 0.0009 1.03 0.3215
0.3630 X
Hari ke 3 6
Sumber keragarnan
Db
KT
Fhic
40.96575000 11.70588673
29 -82::; 8.52
JK
Dosis tinier Kuadratik
4 1 1
53.19767429 40.96575000 11.70588673
Total
34
94.42598857
Keteranean : *) nyata pada taraf ( P < 0. 05)
**) sangat nyata pada taraf
( P .c 0 . 01)
Pr> F
O.OOOI 0.0066
Hari k c 5 4
JK
KT
1
51.23269714 37.56626286 8.45192245
37.56626286 8,45192245
34
92.45275429
Db
JK
~ukber keragaman
Db
Dosis tinier Kuadratik
4 1
Total
Hari ke 72
Sumber keragarnan
Dosis Linier Kuadratik
Total
34 87.30606857
Keterangan : *) nyata pada taraf (P < 0. @ 5 )
* *) sangat nyata pada taraf (P
0.0 1 )
Fw
27.34.'' 6.15
Pr> F
0.0001 0.0190
Lampiran 1 4 a. Kandungan kolesterol serum (mddl) pada setiap perlakuan selama 72 hari percobaan. Dosis APM (mg/kg pakan
Rataan
Rataan
Rataan
Periode pengamatan (hari) 0
125.45
18
128.49
150.88
186.19
36
186.11
218.78
54
72
176-10
176.77
175.75
168..0 2
Lampiran 14 b. Daftar sidik ragam kandungan kolesterol
serum.
Hari k e 18 Surnber keragaman
Db
JK
Dosis Linier Kuadratik
4 1
1
11309.68903 3324.391159 2678.544844
Total
39
67585.15856
KT
F,,
3324.391159 2.07 2678.544844 1.67
Pr>F
0.15931 0.2053 ',
Hari ice 3 6
JK
KT
Sumber Keragaman
Db
Dosis Linier Kuadratik
4 1 1
36503.34926 920.04612 920.04612 21306.52814 21306.52814
39
79505.94625
Total
*) nyata pada taraf (P
< 0.0 5 )
**) sangat nyata pada taraf (P
< 0. 0 1)
Fhjt
Pr > F
0.77 0.3877 26.03*"0.0001
Hari ke 54
K
Sumber keragaman
Db
Dosis Linier Kuadratik
4 1 1
Gal at
35 40409.77185
Total
39 50003.35557
6952.73239 2011.63900 6421.42089
KT
Pr > F
F ,
2011.639005 1.74 0.1954 6421.420895 5.56**' 0.0241
Hari ke 7 2 Sumber keragaman
Db
JK
KT
Dosis Linier Kuadratik Galat
35
Total
39 500003.35558
45553.535275
1301.529579
Keteranean : * ) nyata pada taraf (P < 0.05)
**)
sangat nyata pada taraf (P < 0.01)
Fhit
Pr>F
Lampiran 15 a. Kandungan vitamin C ovarium (ug/g) pada setiap perlakuan selama 72 hari percobaan.
Periode pengamatan (hari)
Dosis APM (mg/kg pakan 1
0
Rataan
63.96 69.46 97.02 76.81 105.15 92.01 148.67 115.27
-
Rataan 1500
36
54
72
382.17 316.81 305.45 294.15 330.62 299.04 397.58 254.56
258.19 283.44 217.14 391.85 335.85 251.66 387.62 241.49
322.55
295.90
72.05
750
-
18
-
139.39 98.50 110.50 116.13 180.49 125.12 138.37 149.37
103.54 134.13 113.11 94.72 93.12 145.35 98.31 164.44 159.C7 169.71
120.87 470.90 367.60 115.47 425.75 324.14 117.60 469.72 392.31 246.29 320.17 386.33 180.46 455.86 368.06 202.18 402.57 372.55 134.35 377.45 399.49 212.57 382.96 395.14
2250
148.39 164.55 158.11 127.12 149.80 180.57 133.56 18a.41
257.18 145.53 259.01 124.16 227.06 303.87 230.46 116.76
460.17 465.17 481.12 410.00 453.76 396.88 362.75 393.66
311.25 395.28 386.62 377.57 303.40 358.06 379.55 390.49
Rataan .
168.31
224.25
422.93
370.27
3 000
160.94 127.40 147.30 193.61 203.93 176.52 208.00 233.10
255.00 229.97 269.49 156.18 218.75 203.36 281.64 293.38
449.89 442.00 468.30 393.05 429.00 390.13 388.43 392.84
303.24 381.12 308.36 362.36 353.37 356.17 354.45 362.01
Rataan
188.85
238.51
358.43
349.26*
-
Lampiran 15 b . Daftar sidik ragam kandungan vitamin C ovarium. setiap periode pengarnatan. Hari ke 18
Surnber Keragaman
Db
Dosis Linier Kuadratik
4 1 1
Total
JK
71205.60970 70860.94105 70860.94105 322.66080 322.66080
97.65"' 0.44
0.0001 0.9847
3 9 96603.29115
Hari ke 3 6
Sumber Keragarnan
Db
JK
Dosis Linier Kuadratik
4 1 1
Gal at
35
95756.0332
Total
39
295341.6759
199585.6427 186884.3778 186884 -3778 9690.2283 9690.2283 2735-8867
Keteranean : * ) nyata pada taraf
*+)
(P< 0.05) sangat nyata pada taraf (P < 0.01 )
68.31*" 3.54
4
0.0001 0.0582
Hari ke 54
Sumber Keragaman
Db
JK
KT
Dosis Linier Kuadratik
4 1 1
Total
3 9 963372.1249
Y =
897422.4496 579340.3762 293722.1244
-
291.0727
t
F,t
Pr>F
579340.3762 307.37*-' 0.0001 293722.1244 155.80'*' 0.0001
392.3617X
-
51.2105
X"
Hari k e 7 2
Sumber Keragaman
Db
JK
KT
Dosis Linier Kuadratik Galat
35
46490.5702
Total
39
738337.1747
Keteranean
1328.3020
:
*) **)
nyata pada taraf (P < 0 .05 ) sangat nyata pada taraf (P < 0.0 1 )
Fw
Pr> F
Lampiran 1 6 a. Kandungan Vitamin C hati ( u d g ) pada setiap perlakuan selarna 72 hari percobaan
.
1)osis APM ( ~ n g / k gpakan)
Periode pengarnatan (hari) 0
18
36
54
72
750
87.79 81.32 88.07 110.11 137.97 92.01 84.02 120.93
121.12 108.12 99.14 111.12 130.99 140.72 217.92 137.56
140.11 162.17 172.17 112.15 160.92 172.19 160.19 142.21
187.34 180.59 219.50 192.14 235.85 287.62 251.66 191.21
Rataan
100.28
133.34 152.76
1500
99.19 146.91 99.52 97.02 125-60 118.05 89.02 91.35
130.20 180.68 112.27 174.45 205.51 226.00 112.07 174.25
248.19 262.03 178.11 164.12 209.11 252.31 161.39 159.14
249.12 212.36 327.05 206.22 227.91 306.40 225.16 262.12
108.33
164.42 204.30
252.05
Rataan
Rataan
218.21
-
Rataan
116.92
188.65
256.01
273.89
Rataan
124.87
193.54
241.29
293.03
Lampiran 16 b. Daftar sidik ragam kandungan vitamin C hati. setiap periode pengamatan
.
Hari ke 18
Sumber Keragaman
Db
Dosis Linier Kuadratik
4
I 1
JK
26857.77752 21522.70440 3819.59360
KT
21522.70440 3819.59360
F h i t
54-42"' 9.66++'
Pr > F
O.OOO~ 0.0001
Hari k e 3 6
Sumber Keragaman
Db
JK
KT
Dosis Linier Kuadratik
4 1 1
140611.7999 12487 -8564 122487.8564 15117.6204 5117.6204
Total
39
215660.6743
*) nyata pada taraf (P <
** )
0.05 )
sangat nyata pada taraf (P < 0.0 1 )
57.12+"' 7.05""
0.0001 0.0018
Hari ke 54
Db
JK
Galat
35
34773.8100
Totai
39 301231.9045
Sumber Keragaman
KT
Dosis Linier Kuadratik 993.5374
Hari ke 72
Sumber Keragaman
DB
JK
Dosis Linier Kuadratik
4 1 1
Total
39 458693.5806
403098.4338 230433.2184 151919.3304
KT
230433.2184 151919.3304
Keteranean :
< 0.05) sangat nyata pada taraf (P < 0.01)
* ) nyata pada taraf (P
** )
145.07-*' 95.64")
0.0001 0.0001
Lampiran 17. Prosentase (%) komposisi oosit berdasarkan (mm) pada awal percobaan
.
NO
IGS
diameter
diameter oosit (mm)
(8)
< 0.40
0.41-0.80
0.81-1.80
1.81-2.20
Lampiran 18. Prosentase (%) komposisi oosit berdasarkan diameter (mm) setiap perlakuan pada hari ke 1 8 . Dosis APM (mg'kg ~ a k a n )
IGS
diameter oosit (mm) C
0.40
0.41-0.80
Rataan
53.67
45.33
Rataan
44.00
43.20
0.81-1.80
1.81-2.20
1
11.60
1-20
Rataan
49.0
46.50
4.50
0
Rataan
46.8
42.0
11.20
0
Rataan
42.67
57.33
Keteranean :
-
-
contoh tidak cukup
* = jantan '
0
0
Lampiran 19. Prosentase (%) komposisi oosit berdasarkan diameter (mm) setinp perlakuan pada hari k e 3 6 . Dosis APM (mglkg pakan)
IGS
diameter oosit (mm) < 0.40
Rataan
44.28
Rataan
39.77
0.41-0.80
45.86
43.50
0.81-1.80
1,81-2-20
9.57
0.29
15.25
1.88
Rataan
38.9
Rataan
Rataan
Keteranean
-
-
20.50
3.20
32.44
42.44
26.22
2.66
36.0
44.23
17.55
2.22
;
contoh tidak cukup
* = jantan '
37.21
Lampiran
2 o . Prosentase (%) komposisi oosit berdasarkan diameter (mtn) setiap perlakuan pada hari ke 5 4 ,
Dosis APM (mgkg pakan)
ICS
< 0.40
0.41-0.80
diameter oosit (mm) 0.81-1.80 1.81-2.20
Rataan
36.33
37.33
20.45
5.89
Rataan
24.22
30.67
35.44
9.67
Rataan
25.82
Rataan
Rataan
-
= =
33.50
12.0
24.78
30.78
21.55
22.89
29.00
2 8 -67
30.22
12.11
Keteranean :
*
28-62
contoh tidak cukup jantan
Lampiran 2 1. 1'1-osr~lrase(%) ko~nposisioosit berdasarkan diameter (IIIIII) sctiap perlakuan pada hari ke 7 2 .
Dosis APM (mglkg pakan)
IGS
Rataan 750
Rataan
diameter oosit (mm) <
0.40
31.45
2.61 2.96 3.04 2.56 1.67 1.66 1.72 1.52 1.68
17 19 21 17 23 20 25 22 22 20.67
0.41-0.80
32.77
0.81-1.80
21.55
1.81-2.20
14.22
Rataan
19.30
22.80
Rataan
20.33
3.33
Rataan
19.22
28.00
'
Keterangan :
-
*
=
c o ~ ~ tidak ~ o h cukup
- J." 1 l I ' l l ~ -~
13 -90
44.00
14.55
43.00
18.68
34.10
Lampiran
22 a. Daftar sidik ragam daya tetas telur (%)
Sumber keragaman
JK
Db
KT
Pr> F
=hit
Dosis Linier Kuadratik
4 1 1
3.6622357 2.3017533 1.2982810
2.3017533 1.2982810
Gal at
9
1.0076500
0.1119611
13
4.6698857
20.56" 11.60*'
0.0014 0.0078
Total Keteranean :
*) nyata pada taraf (P
< 0. 05) sangat nyata pada taraf (P c 0.0 1)
**)
Lampiran
Sumber keraga~nan
2 2 b. Daftar sidik ragam rataan diameter telur (mm)
JK
Db
KT
Dosis Linier Kuadratik
4 1 1
0.0973857 0.0565785 0.0306672
Total
13
0.1046357
Pr>F
&it
0.0565785 0.0306672
70.24"' 38.07'*'
Keteranrran :
*) **)
nyata pada taraf (P < 0.05) sangat nyata pada taraf (P < 0.0 1)
Y = 1.804
+
0.214 X
-
0.0281
X'
0.0001 0.0002
Lampiran 22 c. Dafiar sidik ragam panjang totaI larva (mm) Db
.rK
Dosis Linier Kuadratik
4 1 1
0.5917381 0.2716071 0.2728281
Total
13
0.8875214
Sumber keragaman
KT
Pr> F
Fct
0,2716071 8.26'*' 0.2728281 8-30.'
0.0143 0.0449
Keteranrzaa :
*) nyata pada taraf (P i * *) sangat nyata pada taraf (P
0.05 )
.
< o oi)
Lampiran 2 2 d. Dafiar sidik ragarn bobot larva (mg) Sumber keragaman
Db
Dosis Linier Kuadratik
4
1 11
3.5781714 1.5378285 1.6375540
1.5378285 9.87-' 1.6375548 10.51+'
Galat
9
1.4028000
0.1558661
Total
13
4.9809714
IK
KT
Keteranaan : *) nyata pada taraf (P < 0.05)
**) sangat nyata pada taraf (P <
0.01)
Fhi
t
Pr> F
0.0119 0.0101
Lampiran 22 e. Daftar sidik ragarn abnorrnalan larva (%)
Sumber keragarnan
Dosis
Db
JK
Linier Kuadratik
4 1 1
22.2194047 16.5100960 5.0252005
Total
13
25.7417214
KT
16.51009603 5.02520057
Keteranrran :
*) nyata pada taraf (P c 0. 05) **) sangat nyata pada taraf (P c. 0.01)
'hit
Pr > F
42.19'*' 0. 0001 12.84&' 0.0059
Lampiran 2 3 . Kandungan vitamin C telur (awal), larva, pascalarva pada setiap perlakuan (ug/g).
Stadium
Ulangan 0
Telur
1 2 3
Rataan
7 50
Dosis APM (mg/kg pakan) 1500 2250
3000
12.12 9.96 0.00
105.12 96.19 112.27
185.16 185.72 192.12
196.64 186.72 176.76
187.00 174.96 192.13
7.36
104.52
187.66
186.70
184.69
2.34
72.82
155.05
164.89
171.13
0.00
58.31
121.54
141.23
137.39
Larva
Rataan
Rataan Keteranean =
-
=
data hilang
Lampiran 2 4 .
Dosis (mg/kg pakan)
Sifat kimia air dalam wadah pemeliharaan percobaan .
Oksigen (PP~) min mak
Ammonia (PP~) min mak
Sifat kimia air Nitrit (PP~) rnin mak
ikan nila selama
PH (unit) min mak
Kesadahan (CaC03) min mak
I.empiran
25
.
Kandungan total lipidn, lipida no11 polar dan polar ova-ium, telur dau hati dari bnduk T K U IV
ilosis APM
Contoh I
Contoh 2
liataar~
Tclur Dosis APM
Contoh 1
Contoh 2
Rataan
(nlgk6 ~"k"11)
LC 0
18.06 69.71 30.29
D o s i s APM (mg/kg palran)
0
LT
LP
LN
18.02 69.63
Contoh 1 IHS
LT
1.16 8.69
1.N
LN
LP
LN
I-T 18.04
30.27
Contoh 2
Lt'
IHS
LT
62.60 37.40 1.21 8 . 9 5
69.67
LP 30.27
Iiataan
1-N
I.\'
72.60
27.40
Ll'
L,N
LP
8.82 68.10 31.90
