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PENGUJIAN FORMULA PUPUK HAYATI RHIZOBAKTERI TERHADAP PERTUMBUHAN DAN HASIL TANAMAN JAGUNG (Zea mays) DI LAHAN KERING SKRIPSI
Diajukan Untuk Memenuhi Sebagai Persyaratan Menyelesaikan Studi Sarjana S-1 Program Studi Agroteknologi
Oleh : DIKSAN OKKY RAMADHAN Nim. 201110200311003
Kepada JURUSAN AGRONOMI PROGRAM STUDI AGROTEKNOLOGI FAKULTAS PERTANIAN PETERNAKAN UNIVERSITAS MUHAMMADIYAH MALANG 2015
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RIWAYAT HIDUP Penulis dilahirkan di Sambirejo, sebuah Desa yang berada di Kecamatan Jiwan, Kabupaten Madiun– Jawa Timur pada tanggal 20 Maret 1993, sebagai Putra pertama dari Kardiono dan Umi Chasanah. Penulis menyelesaikan pendidikan Sekolah Dasar Negeri 02 Jambu pada tahun 2005. Pada tahun yang sama penulis masuk Sekolah Menengah Pertama Negeri 2 Pare dan lulus pada tahun 2008, pada tahun yang sama pula penulis masuk Sekolah Menengah Atas Negeri 1 Pare dan lulus pada tahun 2011. Pada tahun yang sama pula penulis melanjutkan pendidikan di Jurusan Agroteknologi Fakultas Pertanian-Peternakan Universitas Muhammadiyah Malang. Penulis melaksanakan Praktek Kerja Lapangan (PKL) di PT. Bisi International Tbk. Kediri pada tahun 2013.
KATA PENGANTAR Syukur alhamdulillah penulis panjatkan terhadap Allah SWT, berkat rahmat dan karunia-Nya penulis dapat menyelesaikan laporan penelitian skripsi dengan judul “Pengujian Formula Pupuk Hayati Rhizobakteri terhadap Pertumbuhan dan Hasil Tanaman Jagung (Zea mays) pada Lahan Kering”. Pada kesempatan ini, penulis menyampaikan ucapan terima kasih dan penghargaan kepada seluruh pihak terutama : Yang Terhormat : 1. Ayahku (Kardiono), Ibuku (Umi Chasanah) serta saudaraku (M. Tsalsa Afriansyah) yang selaku memberikan motivasi dan doa. 2. Dr. Ir. Damat, MP. selaku Dekan Fakultas Pertanian-Peternakan. 3. Dr. Ali Ikhwan, MP. selaku Ketua Jurusan Agronomi dan Pembimbing utama. 4. Ir. Sufianto, MM. selaku Pembimbing pendamping. 5. Teman-teman angkatan 2010 (Joko Ari Prasetyo dan Syahrul Mubarok S.P) dan teman-teman angkatan 2011yang telah berbagi ilmu dan memberikan semangat kepada penulis 6. Temandekatku (Nissa Wahyu Puji Lestari) yang telah membantu penulis serta memberikan semangat dan motivasi untuk menyelesaikan draf skripsi ini. Penulis menyadari bahwa laporan ini masih jauh dari sempurna, oleh karena itu kritik dan saran sangat diharapkan. Semoga laporan ini dapat memberikan manfaat dan masukan bagi semua pihak.
Malang, 10 September2015 Penulis
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DAFTAR ISI Isi
Halaman
KATA PENGANTAR ..........................................................................
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DAFTAR ISI ........................................................................................
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DAFTAR TABEL ................................................................................
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DAFTAR GAMBAR ............................................................................
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DAFTAR LAMPIRAN ........................................................................
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RINGKASAN .......................................................................................
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I. PENDAHULUAN ............................................................................
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1.1. Latar Belakang .........................................................................
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1.2. Tujuan ......................................................................................
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1.3. Rumusan Masalah ...................................................................
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1.4. Hipotesis. .................................................................................
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II. TINJAUAN PUSTAKA ................................................................
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2.1.Potensi Rhizobakteri pada Tanaman ..........................................
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2.2.Rhizobakteri sebagai Sumber Hayati pada Tanaman..................
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2.3.Peran Rhizobakteri pada Tanaman.............................................
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2.4. Botani Tanaman Jagung (Zea mays) .........................................
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III. METODE PENELITIAN ..............................................................
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3.1.Tempat dan Waktu Penelitian ....................................................
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3.2.Alat dan Bahan .........................................................................
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3.3. Metode Kerja ...........................................................................
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3.4. Pelaksanaan Penelitian .............................................................
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3.4.1. Tanaman Jagung. ............................................................
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3.4.1.1.Penyiapan Lahan. .....................................................
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3.4.1.2. Penanaman ..............................................................
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3.4.1.3. Pemeliharaan...........................................................
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3.4.1.3.1. Pengairan ........................................................
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3.4.1.3.2. Pemupukan .....................................................
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3.4.1.3.3. Penyulaman ....................................................
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3.4.1.3.4. Penjarangan ....................................................
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3.4.1.3.5. Penyiangan ......................................................
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3.4.1.3.6. Pengendalian Hama dan Penyakit ....................
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3.4.1.3.7. Pemanenan ......................................................
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3.5. Pengamatan. .............................................................................
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3.6. Analisis Data ............................................................................
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IV.HASIL DAN PEMBAHASAN .......................................................
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4.1 Hasil Pengamatan .....................................................................
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4.1.1. Tinggi Tanaman ..............................................................
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4.1.2. Luas Daun .......................................................................
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4.1.3. Berat Segar dan Berat Kering Jagung ..............................
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4.1.4. Panjang dan Berat Tongkol .............................................
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4.1.5. Berat Segar dan Kering Akar ...........................................
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4.1.6. Berat Pipil Jagung per Tongkol .......................................
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4.2 Pembahasan .............................................................................
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V. KESIMPULAN DAN SARAN ........................................................
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5.1 Kesimpulan ..............................................................................
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5.2 Saran ........................................................................................
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DAFTAR PUSTAKA ...........................................................................
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LAMPIRAN .........................................................................................
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DAFTAR TABEL
Tabel
Teks
Hal
1. Uji Rata-rata Tinggi Tanaman Jagung dengan Pemberian Formula Pupuk Rhizobakteri pada Berbagai Umur Pengamatan .
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2. Uji Rata-rata Luas Daun Jagung dengan Pemberian Formula Pupuk Rhizobakteri pada Berbagai Umur Pengamatan ...............
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3. Uji Rata-rata Berat Segar Tongkol Jagung dan Berat Kering Tongkol Jagung dengan Pemberian Formula Pupuk Rhizobakteri pada Berbagai Umur Pengamatan ..........................
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4. Uji Rata-rata Panjang dan Berat Tongkol Jagung dengan Pemberian Formula Pupuk Rhizobakteri pada Berbagai Umur Pengamatan ................................................................................
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5. Uji Rata-rata Berat Segar dan Kering Akar Jagung dengan Pemberian Formula Pupuk Rhizobakteri pada Berbagai Umur Pengamatan ................................................................................
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6. Uji Rata-rata Berat Pipil Jagung per Tongkol dengan Pemberian Formula Pupuk Rhizobakteri pada Berbagai Umur Pengamatan .
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DAFTAR GAMBAR
Gambar
Teks
Hal
1. Denah Percobaan......................................................................
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DAFTAR LAMPIRAN
Lampiran
Teks
Hal
1. Hasil Analisis Tinggi Tanaman pada Semua Pengamatan .......
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2. Hasil Analisis Luas Daun pada Semua Pengamatan ...............
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3. Hasil Analisis Berat Segar dan Berat Kering Jagung ..............
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4. Hasil Analisis Panjang dan Berat Tongkol Jagung .................
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5. Hasil Analisis Berat Segar dan Kering Akar ...........................
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6. Hasil Analisis Berat Pipil Jagung per Tongkol .......................
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7. Dokumentasi Bahan ...............................................................
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8. Dokumentasi Kegiatan ...........................................................
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9. Dokumentasi Pengamatan ......................................................
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DAFTAR PUSTAKA
Aak., 1996. Teknik Bercocok Tanam Jagung. Kanisiun, Yogyakarta. Adesina, V.M. Felicia. 2007. Characterization of bacterial antagonists of Rhizoctonia solani and arium oxysporum from six European soils and their potentialapplication for biological control. Dissertation. Germany: Universitat Carolo-Wilhelmina. Agustisyah, S. Ilyas, Sudarsono, dan M. Mahmud. 2013. Karakterisasi rizobakteri yang berpotensi mengendalikan bakteri Xanthomonas oryza pv. Oryzae dan meningkatkan pertumbuhan tanaman padi. HPT Tropika 13(1). Ahmad F, Ahmad I, Khan MS. 2005. Indoleacetic acid production by the indigenous isolates of Azotobacter and fluorescent pseudomonad in the presence and absence of tryptophan. Turk. J. Biol. 29:29-34. Akhtar, A., Hisamuddin, M.I. Robab, Abbasi, and R. Sharf. 2012. Plant growth promoting rhizobacteria : An overview. J. National. Production Plant Resources 2 (1) : 19-31. Ashrafi, V. and M.N. Seiedi. 2011. Influence of different plant densitiees and plant growth promoting rhizobacteria (PGPR) on yield and yield antrbutes of corn (Zea maysL.). recent Res. in Sci. and Tech. 3(1): 63-66. Ashrafuzzaman M, F.A. Hossen, M.R. , MdA. Hoque, M.Z. Islam, S.M. Shahidullah, and S. Meon. 2009. Efficiency of plant growth-promoting rhizobacteria (PGPR) for the enhancement of rice growth. Africa Journal of Biotechnology 8(7): 1247-1252. BPS dan Kementerian Pertanian. 2011. Produksi Padi, Jagung, dan Kedelai (Angka ramalan III tahun 2011). (http:// www.bps.go.id/brs_file/aram_lnov11.pdf) 19 Desember 2014 Badan Pusat Statistik. 2011. Produksi padi, jagung, dan kedelai (angka sementara tahun 2010 dan angka ramalan I tahun 2011). Berita Resmi Statistik 14:1-9. Beneduzi. A., A. Ambrosi, and L.M.P. Passaglia. 2012. Plant growth-promoting rhizobacteria (PGPR) : Their potential as antagonists and biocontrol agents. Genetic andmolecular Biology 35 (4):1044-1051. Biari, A., A. Gholami, and H.A. Rahmani. 2008. Growth promotion and nautrient uptake of maize (Zea mays) by application of plant growt promotion rhizobactera in arid region of Iran. Bio. Sci. 8(6):1015-1020.
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Botelho, G.R. ., K.V.B.R Tilak, (2006). Fluorescent Pseudomounads Associated With The Rhizosphere Of Crops – An Overview. Brazilian Journal of Microbiology. 37, 401-406. Das, A.J., M. Kumar, and R. Kumar. 2013. Plant growth-promoting rhizobacteria (PGPR) : An Alternatif of chemical fertilizer for sustainable, environment friendly agriculture. Research Journal of Agriculture and Forestry Sciences 1(4), 21-23. Desmawati, 2006. Pemanfaatan Plant Growth Promoting Rhizobacter (PGPR) Prospek Yang Menjanjikan dalam Berusaha Tani Tanaman. POPT Direktorat Perlindungan Tanaman Hortikultura dan Ditjen Hortikultura. http://ditlin.hortikultura.deptan.go.id/tulisan/desmawati.htm (Accessed 19 Desember 2014) Diby, P, and Y.R. Sarma 2006.Plant growth-promoting rhizobacteria (PGPR) mediated root proliferation in black peper (Piper nigrum L.) as evidenced through GS root software. Phytopatology and Pland Protection 39 : 1-4. Diby, P., K.A Salju, P.J. Jisha, Y.R. Sarma, A, Kumar, and M, Anandaraj. 2005. Mycolytic enzymes produced by Pseudomonas fluorescens and Trichoderma spp. against Phytopjthora capsici, the foot rot pathogen of black peper (Piper nigrum L.). Ann. Microbiol 55 : 129-133. Dobbelaere, S., J. Vanderleyden, and Y. Okon. 2003. Plant growth-promoting effects of diazotrophs in the rhizosfer. CRV Review Plant Science (22) : 107-149. Elango R., R. Parthasarathi, and S. Megala. 2013. Field level studies on the association of plant growth promoting rhizobacteria (PGPR) in Gloriosa Superba L. rhizosphere. Indian Streams Research Journal 3(10): 1-6. El-Azeem SAMA, T.A. Mehana, and A.A. Shabayek. 2007. Some plant growth promoting traits of rhizobacteria isolated from Suez Canal region, Egypt. African Crop Science Conference Proseedings 8:1517-1525. Estrada, J. D., M. S. Rossi, J. A. Andres, M. Rovera, N.S. Correa, and S.B. Rosas. 2004. Greenhouse evaluation of Pseudomonas aurantiaca formulated as inoculat-ion for the biocontrol of plant pathogen fungi. http://www.ag.auburn.edu/Argentina / pdf manuscripts/ Estrada. Gholami A., A. Biari, and S. Nezarat. 2008. Effect Of Seed Priming With Growth Promoting Rhizobacteria At Different Rhizophere Condition On Growth Parameter Of Maize. International Meeting On Soil Ferti;ity Land Management and Agroclimatology. Turkey P: 851-856.
34
Haas, D. and G. Devago. 2005. Biologycal Control Of Soil-Borne Pathogens by Fluorescens Pseudomonads. Nature Reviews Microbiology. 1,1-13. Jayasekhar, M., K. Manonmani, and C.G. Justin. 2008. Development of integrated biological strategy for the management of stem rot disease (Fusarium oxysporum f.sp. vanillae) of Vanilla. Agriculture Science Digest 28(2) : 109-111. Joo GJ, Kim YM, Kim JT, Rhee IK, Kim JH, Lee IJ. 2005. Gibberellinsproducing rhizobacteria increase endogenous gibberellins content and promote growth of red peppers. J Microbiol. 43(6): 510-5. Kasno A. 2010. Nutrient Balance at Integrated Nutient Management on Lowland Rice Which Is Dominated By 1:1 Clay Mineral for High Potential Rice Yields. Jurnal Tanah Tropika 15(2):119-126. Kishore, G.K., S. Pande, and A.R. Podile. 2005. Phylloplane bacteria increase seedling emergence, growth and yield of field –grown groundnut (Arachis hypogaea L). http://www.blackwell-synergi. Com/ doi / abs. Kloeper J.W. R. Rodriguez-Ubana, G. W. Zehnder, J.F. Murphy, E. Sikora and C Fernandez. 1999. Plant root-bacterial interactions in biological control of soilborne diseases and potential extension to systemic and foliar diseases. Australasian Plant Pathology 28:21-26. Kloeper J.W, Lifshitz R, Zablotowicz RM. 1989. Free living bacterial inocula for enhancing corp productivity. Trends Biotechnol 7:39-43 Lakitan. B. 2001. Dasar-dasar Fisiologi Tumbuhan. Rajawali Pers. Jakarta Loon LC. 2007. Plant responses to plant growth-promoting rhizobacteria. Eur J. Plant Pathology 119:243-254. Mamotonto, R. 2005. Pengaruh dosis pupuk majemuk NPK Phonska terhadap pertumbuhan dan produksi jagung maniz (Zea maysScchata slurt) . skripsi. Fakultas Pertanian Universitas Icshan, Gorontalo Maunuksela, L. 2004. Molecular And Physiological Characterization Of Rhizosphere Bacteria And Frankia In Forest Soils Devoid of Actinorhizal Plants. Dissertationes Biocentri Wikki Universitatis Helsingiensis. http://ethesis. Helsinki.fi. / julkaisnt/ mat / manuksela/ molecula.pdf. (19 Juli 2008) Paul, E.A. 2007. Soil Microbiology, Ecology and Biochemitry 3rd Edition. Elsivier. United States of America. Phukan, I., M. Madhab, M. Bordoloi, S.R. Sarmah, P. Dutta, R. Begum, A. Tanti, S. Bora, S.C. Nair, S. Rai, S. Debnath, and B.K. Barthakur. 2012.
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Exploitation of RPTT microbes of tea for improvement of plant growth and pest suppression : A novel approach. Two and Bud. 59:69-2012. Podile, A.R. and G.K. Kishore. 2007. Plant Growth-Promoting Rhizobacteria.p. 195-230. In : Nanamarickam, S.S. (ed). Plan-Associated Bacteria. Springer Saddler, G.S., 2005. Management of Bacteria Wilt Disease. P.121-132. In : In : Allen, C., Prior, P., Hayward, A.C.Bacterial Wilt Disease and theRalstoria solanacearum Species Complek. The American Phytopathological Society Press. Rahni, N.M. 2012. Efek Fitohormon Terhadap Pertumbuhan Tanaman jagung (Zea mays). Artikel Dosen Agroteknologi Universitas Haluoleo. Rahni, Mila Nini. 2012. Efek Fitohormon PGPR Terhadap Pertumbuhan Tanaman Jagung (Zea mays). Jurusan Agroteknologi Universitas Haluoleo. Rokhzadi, A., A. Asgharzadeh, F. Darvish, G.N. Mohammadi and E.E Majidi. 2008. Influence of Plant Growth-Promoting Rhozobacteria on dry matter accumulation and yield of chickpea (Cicer arietinum L) under field conditions. American Eurisian J. Agric. & Environ. Sci. 3(2): 253-257 Rubatzky, V.E dan M. Yamaghuci., 1998. Sayuran Dunia 1. Prinsip, Produksi dan Gizi. ITB Press, Bandung. Rudresh, D.L., M.K. Shivaprakash and R.D. Prasad. 2005. Effect of combined application of rhizobium, phospate cedublizing bacterium and yield of chickpea (Cicer arietnum L). Applied Soil Ecol. 28: 139-146 Rukmana, H.R. 1997. Usaha Tani Jagung. Kanisius, Jogjakarta. Sharifi, R.S., K. Khavazi, and A. Gholipouri. 2011. Effect of seed priming with plant grownh promoting rhizobacteria (PGPR) on dry matter accumulation and yield of maize (Zea mays) hibrids. International Research Journal of Biochemistry and Biofarmatics. 1(3): 076-083. Sharma, A, K.2002. Organic farming, Central Arid Zone Research institute Jidhpur. Agrobios. India Sitompul, S. M., dan B. Guritno. 1995. Analisis Pertumbuhan Tanman. Gadjah Mada University Press. Yogyakarta. Soedarsono, Y. 1997. The Potency of Drought-Tolerant Rhizobacteria as Inoculant for Gogo Rice. Indonesia Biotecnology Conference. Jakarta Soemartono, S. 1995. Cekaman lingkungan, tantangan pemuliaan tanaman masa depan. Prosiding Simposium Pemuliaan Tanaman III, PERIPI Komda Jawa Timur. Hal 1-12.
36
Sutariati, GAK, Widodo, Sudarsono, Ilyas S. 2006. Pengaruh perlakuan rhizobakteri pemacu pertumbuhan tanaman terhadap viabilitas benih serta pertumbuhan bibit tanaman cabai. Bul. Agron. 34(1): 46-54. Sutariati Gak. 2005. Perlakuan Benih dengan Agens Biokontrol untuk Pengendalian Penyakit Antraknosa, Peningkatan Hasil da Mutu Benih Cabai. Disentasi. Program Pascasarjana Institut Pertanian Bogor. Thakuria, D., N.C. Talukdar, C. Goswami, S. Hazarika, R.C. Boro, and M.R. Khan. 2004. Characterization and screening of bacteria from rhizosphere of rice grown in acidic soils of assam. Curren Sci. 86: 978-985. http://www.bio.uu.nl/ fytopath/ pdffiles/ Bookch.vanLoon 2003 (17 Maret 2010) Thanh, D.T., L.T. Tarn., N.T. Hanh, N.H. Tuyen, Bharathkumar, Srinivasan, S.Y. Lee and K.S Park. 2009. Biological control of soilborne diseases on tomato, potato and black pepper by selected RPTT in greenhouse and field in Vietnam. Plant Pathology Journal 25(3) : 263-269. Timmusk S, Grantcharova N, Wagner EGH. 2005. Paenibacillus polymya invades plant roots and forms biofilms. Applied and Environmental Microbiology 71(11): 7292-7300. Wang, Shui, H. Wu, J. Qiao, L. Ma, J. Liu, Y. xia and x. Gao. 2009. Molocular mechanism of plant growth promoting and induced systemic resistance to tobacco mosaic virus by Bacillus spp. Journal Microbiology Biotechnology. Http://www.kormb.or.kr/storage/Press/JMB/19 0/1056/articlefile/articlefile/article.pdf(2 Agustus 2013). Warisno. 1998. Budidaya Jagung Hibrida. Kanisius, Yogyakarta. Yazdani, M., M.A. Bahmanya, H. Pirdashti, and M.A. Esmaili. 2009. Effect of Phosphate solubilization microorganisme (PSM) and plant growth promoting rhizobacteria (PGPR) on yield and yield components of corn (Zea mays). Int. J. of Biol. And Life Sci. 5(2):80-82. Zaidi, A., M.S Khan., M. Amil. 2003. Interactive effect of rhizotropic microorganisms on yiled and nutrient up take of chikpea (Cicer arietinum L). European J. Of Agro. 19: 15-21
