Lampiran 1 Hasil Pengukuran Jumlah Limfosit dan Makrofag
Kelompok K1 K2 K3 K4 K5 P1 No. 1 P 1 No. 2 P 1 No. 3 P 1 No. 4 P 1 No. 5 P 2 No. 1 P 2 No. 2 P 2 No. 3 P 2 No. 4 P 2 No. 5 P 3 No. 1 P 3 No. 2 P 3 No. 3 P 3 No. 4 P 3 No. 5
Jumlah limfosit 6 7 6 6 7 11 12 12 12 12 14 15 16 16 16 20 21 21 22 22
Jumlah makrofag 2 3 2 3 3 5 5 6 6 6 7 8 9 10 9 14 15 16 16 15
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Lampiran 2. Hasil Pengolahan data penelitian menggunakan SPSS 17.0
Explore Limfosit Descriptives Limfosit
Kelompok K
P1
P2
P3
Mean 95% Confidence Interval for Mean 5% Trimmed Mean Median Variance Std. Deviation Minimum Maximum Range Interquartile Range Skewness Kurtosis Mean 95% Confidence Interval for Mean 5% Trimmed Mean Median Variance Std. Deviation Minimum Maximum Range Interquartile Range Skewness Kurtosis Mean 95% Confidence Interval for Mean 5% Trimmed Mean Median Variance Std. Deviation Minimum Maximum Range Interquartile Range Skewness Kurtosis Mean 95% Confidence Interval for Mean 5% Trimmed Mean Median Variance Std. Deviation Minimum Maximum Range Interquartile Range Skewness Kurtosis
Lower Bound Upper Bound
Lower Bound Upper Bound
Lower Bound Upper Bound
Lower Bound Upper Bound
Statistic 6.40 5.72
Std. Error .245
7.08 6.39 6.00 .300 .548 6 7 1 1 .609 -3.333 11.80 11.24
.913 2.000 .200
12.36 11.83 12.00 .200 .447 11 12 1 1 -2.236 5.000 15.40 14.29
.913 2.000 .400
16.51 15.44 16.00 .800 .894 14 16 2 2 -1.258 .313 21.20 20.16
.913 2.000 .374
22.24 21.22 21.00 .700 .837 20 22 2 2 -.512 -.612
.913 2.000
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Tests of Normality a
Limfosit
Kelompok K P1 P2 P3
Kolmogorov-Smirnov Statistic df Sig. .367 5 .026 .473 5 .001 .349 5 .046 .231 5 .200*
Statistic .684 .552 .771 .881
Shapiro-Wilk df 5 5 5 5
Sig. .006 .000 .046 .314
*. This is a lower bound of the true significance. a. Lilliefors Significance Correction
25
20
15
6 10
5 K
P1
P2
P3
Kelompok
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NPar Tests Kruskal-Wallis Test
Ranks Limfosit
Kelompok K P1 P2 P3 Total
N 5 5 5 5 20
Mean Rank 3.00 8.00 13.00 18.00
Test Statisticsa,b Chi-Square df Asymp. Sig.
Limfosit 18.144 3 .000
a. Kruskal Wallis Test b. Grouping Variable: Kelompok
NPar Tests Mann-Whitney Test
Ranks Limfosit
Kelompok K P1 Total
N 5 5 10
Mean Rank 3.00 8.00
Sum of Ranks 15.00 40.00
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Test Statisticsb Mann-Whitney U Wilcoxon W Z Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
Limfosit .000 15.000 -2.739 .006 .008
a
a. Not corrected for ties. b. Grouping Variable: Kelompok
NPar Tests Mann-Whitney Test
Ranks Limfosit
Kelompok K P2 Total
N 5 5 10
Mean Rank 3.00 8.00
Sum of Ranks 15.00 40.00
Test Statisticsb Mann-Whitney U Wilcoxon W Z Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
Limfosit .000 15.000 -2.685 .007 .008
a
a. Not corrected for ties. b. Grouping Variable: Kelompok
55
NPar Tests Mann-Whitney Test
Ranks Limfosit
Kelompok K P3 Total
N 5 5 10
Mean Rank 3.00 8.00
Sum of Ranks 15.00 40.00
Mean Rank 3.00 8.00
Sum of Ranks 15.00 40.00
Test Statisticsb Mann-Whitney U Wilcoxon W Z Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
Limfosit .000 15.000 -2.668 .008 .008
a
a. Not corrected for ties. b. Grouping Variable: Kelompok
NPar Tests Mann-Whitney Test
Ranks Limfosit
Kelompok P1 P2 Total
N 5 5 10
56
Test Statisticsb Mann-Whitney U Wilcoxon W Z Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
Limfosit .000 15.000 -2.730 .006 .008
a
a. Not corrected for ties. b. Grouping Variable: Kelompok
NPar Tests Mann-Whitney Test
Ranks Limfosit
Kelompok P1 P3 Total
N 5 5 10
Mean Rank 3.00 8.00
Sum of Ranks 15.00 40.00
Test Statisticsb Mann-Whitney U Wilcoxon W Z Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
Limfosit .000 15.000 -2.712 .007 .008
a
a. Not corrected for ties. b. Grouping Variable: Kelompok
57
NPar Tests Mann-Whitney Test
Ranks Limfosit
Kelompok P2 P3 Total
N 5 5 10
Mean Rank 3.00 8.00
Sum of Ranks 15.00 40.00
Test Statisticsb Mann-Whitney U Wilcoxon W Z Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
Limfosit .000 15.000 -2.660 .008 .008
a
a. Not corrected for ties. b. Grouping Variable: Kelompok
58
Explore Makrofag Descriptives Makrofag
Kelompok K
P1
P2
P3
Mean 95% Confidence Interval for Mean 5% Trimmed Mean Median Variance Std. Deviation Minimum Maximum Range Interquartile Range Skewness Kurtosis Mean 95% Confidence Interval for Mean 5% Trimmed Mean Median Variance Std. Deviation Minimum Maximum Range Interquartile Range Skewness Kurtosis Mean 95% Confidence Interval for Mean 5% Trimmed Mean Median Variance Std. Deviation Minimum Maximum Range Interquartile Range Skewness Kurtosis Mean 95% Confidence Interval for Mean 5% Trimmed Mean Median Variance Std. Deviation Minimum Maximum Range Interquartile Range Skewness Kurtosis
Lower Bound Upper Bound
Lower Bound Upper Bound
Lower Bound Upper Bound
Lower Bound Upper Bound
Statistic 2.60 1.92
Std. Error .245
3.28 2.61 3.00 .300 .548 2 3 1 1 -.609 -3.333 5.60 4.92
.913 2.000 .245
6.28 5.61 6.00 .300 .548 5 6 1 1 -.609 -3.333 8.60 7.18
.913 2.000 .510
10.02 8.61 9.00 1.300 1.140 7 10 3 2 -.405 -.178 15.20 14.16
.913 2.000 .374
16.24 15.22 15.00 .700 .837 14 16 2 2 -.512 -.612
.913 2.000
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Tests of Normality a
Makrofag
Kelompok K P1 P2 P3
Kolmogorov-Smirnov Statistic df Sig. .367 5 .026 .367 5 .026 .237 5 .200* .231 5 .200*
Statistic .684 .684 .961 .881
Shapiro-Wilk df 5 5 5 5
Sig. .006 .006 .814 .314
*. This is a lower bound of the true significance. a. Lilliefors Significance Correction
15
12.5
10
7.5
5
2.5
K
P1
P2
P3
Kelompok
60
NPar Tests Kruskal-Wallis Test Ranks Makrofag
Kelompok K P1 P2 P3 Total
N 5 5 5 5 20
Mean Rank 3.00 8.00 13.00 18.00
Test Statisticsa,b Makrofag 18.033 3 .000
Chi-Square df Asymp. Sig.
a. Kruskal Wallis Test b. Grouping Variable: Kelompok
NPar Tests Mann-Whitney Test Ranks Makrofag
Kelompok K P1 Total
N 5 5 10
Mean Rank 3.00 8.00
Sum of Ranks 15.00 40.00
Test Statisticsb Mann-Whitney U Wilcoxon W Z Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
Makrofag .000 15.000 -2.694 .007 a
.008
a. Not corrected for ties. b. Grouping Variable: Kelompok
61
NPar Tests Mann-Whitney Test
Ranks Makrofag
Kelompok K P2 Total
N 5 5 10
Mean Rank 3.00 8.00
Sum of Ranks 15.00 40.00
Mean Rank 3.00 8.00
Sum of Ranks 15.00 40.00
Test Statisticsb Mann-Whitney U Wilcoxon W Z Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
Makrofag .000 15.000 -2.660 .008 .008
a
a. Not corrected for ties. b. Grouping Variable: Kelompok
NPar Tests Mann-Whitney Test Ranks Makrofag
Kelompok K P3 Total
N 5 5 10
62
Test Statisticsb Mann-Whitney U Wilcoxon W Z Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
Makrofag .000 15.000 -2.668 .008 .008
a
a. Not corrected for ties. b. Grouping Variable: Kelompok
NPar Tests Mann-Whitney Test
Ranks Makrofag
Kelompok P1 P2 Total
N 5 5 10
Mean Rank 3.00 8.00
Sum of Ranks 15.00 40.00
Test Statisticsb Mann-Whitney U Wilcoxon W Z Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
Makrofag .000 15.000 -2.660 .008 .008
a
a. Not corrected for ties. b. Grouping Variable: Kelompok
63
NPar Tests Mann-Whitney Test Ranks Makrofag
Kelompok P1 P3 Total
N 5 5 10
Mean Rank 3.00 8.00
Sum of Ranks 15.00 40.00
Mean Rank 3.00 8.00
Sum of Ranks 15.00 40.00
Test Statisticsb Makrofag .000 15.000 -2.668 .008
Mann-Whitney U Wilcoxon W Z Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
.008
a
a. Not corrected for ties. b. Grouping Variable: Kelompok
NPar Tests Mann-Whitney Test Ranks Makrofag
Kelompok P2 P3 Total
N 5 5 10
Test Statisticsb Mann-Whitney U Wilcoxon W Z Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)]
Makrofag .000 15.000 -2.635 .008 a
.008
a. Not corrected for ties. b. Grouping Variable: Kelompok
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Lampiran 3. Ethical Clearance
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Lampiran 4 :
Makrofag
Limfosit
Gambar 9 : Sebukan limfosit dan makrofag pada kelompok perlakuan P3 (Pembesaran 400 X)
66
Limfosit
Makrofag
Gambar 10 : Sebukan limfosit dan makrofag pada kelompok perlakuan P2 (Pembesaran 400 X)
67
Makrofag
Limfosit
Gambar 11 : Sebukan limfosit dan makrofag pada kelompok perlakuan P1 (Pembesaran 400 X)
68
Makrofag
Limfosit
Gambar 12 : Sebukan limfosit dan makrofag pada kelompok perlakuan K (Pembesaran 400 X)
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Lampiran 5. Gambar / foto tikus
Gambar 13 : Saat pemberian bubur tumor
Gambar 14 : Terbentuknya jaringan kanker
Gambar 15 : Saat pemberian ekstrak Nigella sativa 70
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