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Lampiran 1. Pembentukan warna pada uji kadar nitrat (A), nitrit (B), dan amonium (C).
A
B
C
Lampiran 2 Kurva standar konsentrasi amonium (A) dan kurva standar konsentrasi nitrit (B)
Kurva Standar Amonium 2.00 1.80 Absorbansi (640 nm)
1.60
y = 11.201x + 0.0514 R2 = 0.9963
1.40 1.20 1.00 0.80 0.60 0.40 0.20 0.00 0
0.02
0.04
0.06
0.08
0.1
0.12
0.14
0.16
Konsentrasi amonium (mM)
A
Kurva Standar Nitrit 2.00
y = 114.81x + 0.224 R2 = 0.9087
1.80 1.60 Abs or bans i
1.40 1.20 1.00 0.80 0.60 0.40 0.20 0.00
0.000
0.002
0.004
0.006
0.008
0.010
Konsentrasi nitrit (mM)
B
0.012
0.014
0.016
Lampiran 3 Jumlah CFU Isolat HF7 dan LF6 pada pengenceran 10-8
Lampiran 4
Kurva standar pertumbuhan isolat HF7 dan LF6 pada kondisi nitrat medium 49,41 mM
OD (620 nm)
Kurva Standar Pertumbuhan Isolat HF7 0.40 0.35 0.30 0.25 0.20 0.15 0.10 0.05 0.00
y = 0.0073x + 0.0208 R2 = 0.9623
0
10
20
30
40
50
9
Jumlah Organisma ( 10 CFU/ml)
kurva s tandar pertum buhan Isolat LF6
y = 0.091x + 0.0239 R2 = 0.9427
0.30 0.25 0.20 0.15 0.10 0.05 0.00 0
5
10
15
20
Jumlah organisma (109 CFU/sel)
25
30
Lampiran 5
Presentase nitrat tereduksi dan senyawa yang dihasilkan isolat LF6,HF7 dan FR2 pada kadar udara saturasi berbeda dengan inkubasi selama 4 hari suhu ruang (29-31)oC Isolat LF6
Perlakuan udara saturasi
Nitrat nitrat Awal tereduksi
0% 1% 10% 30% 50% 80% 100%
49,41 49,41 49,41 49,41 49,41 49,41 49,41
mM 48,52 47,78 47,50 47,17 46,04 45,34 45,40
% 98,19 96,69 96,12 95,46 93,17 91,75 91,88
Nitrit terbentuk
Amonium terbentuk
Estimasi gas terbentuk
mM 0,05 0,00 0,02 0,01 0,04 0,03 0,03
mM 15,02 6,17 8,94 9,26 12,00 10,02 9,18
mM 33,45 31,61 38,54 37,90 34,01 35,29 36,19
% 0,00 0,00 0,00 0,00 0,00 0,00 0,00
% 30,95 33,85 18,83 19,63 26,06 22,09 20,23
% 68,95 66,15 81,14 80,35 73,86 77,84 79,71
Isolat HF7 Perlakuan udara saturasi
Nitrat nitrat Awal tereduksi
0% 1% 10% 30% 50% 80% 100%
49,41 49,41 49,41 49,41 49,41 49,41 49,41
mM 48,78 47,07 46,13 47,17 45,83 45,84 45,67
% 98,72 95,26 93,35 95,46 92,75 92,77 92,42
Nitrit terbentuk
Amonium terbentuk
Estimasi gas terbentuk
mM % 0,00 0,00 0,00 0,00 0,00 0,00 0,01 0,03 0,02 0,04 0,00 0,00 0,03 0,07
mM % 14,77 30,29 14,19 30,14 13,15 28,50 14,18 30,06 16,19 35,33 14,69 32,05 7,29 15,96
mM 34,01 32,89 32,99 32,98 29,62 31,15 38,35
% 69,72 69,86 71,51 69,91 64,63 67,95 83,96
Isolat FR2 Perlakuan udara saturasi
Nitrat nitrat Awal tereduksi
0% 1% 10% 30% 50% 80% 100%
49,41 49,41 49,41 49,41 49,41 49,41 49,41
mM 48,37 46,63 46,84 48,31 47,89 47,47 44,76
% 97,88 94,36 94,78 97,76 96,91 96,06 90,58
Nitrit terbentuk
Amonium terbentuk
Estimasi gas terbentuk
mM 0,00 0,00 0,02 0,00 0,00 0,00 0,03
mM 6,73 9,02 9,20 8,35 8,90 10,19 7,72
mM 41,64 36,91 37,61 39,96 39,77 38,87 41,38
% 0,00 0,00 0,04 0,00 0,00 0,00 0,06
% 13,91 19,35 19,65 17,28 18,58 21,47 17,25
% 86,09 79,16 80,31 82,72 83,05 81,88 92,44
Lampiran 6 Presentase nitrat tereduksi dan senyawa yang dihasilkan isolat FR1 pada kadar udara saturasi berbeda dengan inkubasi selama 4 hari suhu ruang (29-31)oC Isolat FR1
Perlakuan Nitrat Awal udara saturasi 0% 1% 10% 30% 50% 80% 100%
49,41 49,41 49,41 49,41 49,41 49,41 49,41
nitrat tereduksi
Nitrit terbentuk
Amonium terbentuk
Estimasi gas terbentuk
mM 48,20 47,73 48,01 47,28 47,08 46,35 46,26
mM 1,74 1,88 2,86 2,55 2,54 3,17 4,95
mM 7,09 9,05 6,87 6,08 6,55 6,75 6,50
mM 39,37 36,79 38,28 38,65 37,99 36,43 34,82
% 97,54 96,58 97,15 95,68 95,28 93,79 93,62
% 3,61 3,94 5,96 5,39 5,39 6,84 10,70
% 14,71 18,97 14,30 12,86 13,92 14,56 14,04
% 81,68 89,79 85,87 87,14 86,08 85,44 85,96
Lampiran 7 Perbandingan konsentrasi nitrat tereduksi, nitrit, nitrat, amonium dan estimasi gas yang dihasilkan isolat LF6 dan HF7
Nitrat,nitrit, amonium, gas (mM)
Isolat LF6 50 40 30 20 10 0 0%
1%
10%
30%
50%
80%
100%
Konsentrasi udara saturasi
Nitrat, Nitrit, Amonium, gas (mM)
Isolat HF7 50 40 30 20 10 0 0%
1%
10%
30%
50%
Konsentrasi udara saturasi
Keterangan
Nitrat tereduksi Nitrit terakumulasi Amonium yang dihasilkan Estimasi gas yang dihasilkan
\
80%
100%
Lampiran 8 Perbandingan konsentrasi nitrat tereduksi, nitrit, nitrat, amonium dan estimasi gas yang dihasilkan isolat FR1 dan FR2
Nitrat,nitrit, amonium, gas (mM)
Isolat FR1 50 40 30 20 10 0 0%
1%
10%
30%
50%
80%
100%
Konsentrasi udara saturasi
Nitrat,nitrit,amonium,gas (mM)
Isolat FR2 50 40 30 20 10 0 0%
1%
10%
30%
50%
80%
Konsentrasi udara saturasi
Keterangan
Nitrat tereduksi Nitrit terakumulasi Amonium yang dihasilkan Estimasi gas yang dihasikan
100%
