BIOLOGI - ACHADIAH RACHMAWATI
Electronic noises can spoil the enjoyment of this lecture Please TURN OFF all mobile phones, WAPs and PADs
1
2
SOMATIC AND ASOMATIC CELLS
1
BIOLOGI - ACHADIAH RACHMAWATI
Sel Berdasarkan Jumlah Kromosom 1. Sel somatis (2n) – Sel tubuh (sel yang menyusun seluruh tubuh hewan)
2. Sel asomatis (1n) – Sel kelamin (sel gamet) • •
Spermatozoa → spermatogenesis (testis) Oosit → oogenesis dan folliculogenesis (ovary)
3
Prokaryotic cells Pili: attachment structures on the surface of some prokaryotes Nucleoid: region where the cell’s DNA is located (not enclosed by a membrane) Ribosomes: organelles that synthesize proteins
Bacterial chromosome (a) A typical rod-shaped bacterium
Plasma membrane: membrane enclosing the cytoplasm Cell wall: rigid structure outside the plasma membrane Capsule: jelly-like outer coating of many prokaryotes 0.5 µm Flagella: locomotion organelles of some bacteria
(b) A thin section through the bacterium Bacillus coagulans (TEM)
4
SOMATIC AND ASOMATIC CELLS
2
BIOLOGI - ACHADIAH RACHMAWATI
Eukaryotic cell
5
Animal cell
6
SOMATIC AND ASOMATIC CELLS
3
BIOLOGI - ACHADIAH RACHMAWATI
DNA: Deoxyribonucleic acid Chromosomes Nucleic Acid Nucleotide Nucleosome
2 nm
7
Ribosome produces protein
8
SOMATIC AND ASOMATIC CELLS
4
BIOLOGI - ACHADIAH RACHMAWATI
Two Types of Ribosomes • Free ribosomes- make proteins that stay in the cytoplasm and are used there. • Bound ribosomes- make proteins for export or for membrane construction.
Endoplasmic reticulum (ER)
ER Ribosomes
Cytosol
Free ribosomes
Bound ribosomes Large subunit
0.5 µm TEM showing ER and ribosomes
Small subunit Diagram of a ribosome
9
10
SOMATIC AND ASOMATIC CELLS
5
BIOLOGI - ACHADIAH RACHMAWATI
Sel Spermatozoa
11
Struktur Spermatozoa
12
SOMATIC AND ASOMATIC CELLS
6
BIOLOGI - ACHADIAH RACHMAWATI
Struktur Kepala Spermatozoa • Sebagian kepala diselubungi oleh akrosom yang didalamnya terdapat enzim-enzim yang berguna untuk fertilisasi (pro acrosin dan hyaluronidase) • Enzim ini diaktifkan saat terjadinya reaksi akrosom
13
Ekor Spermatozoa Bagian Middle Piece : • Mitochondrial sheat – menghasilkan energi untuk menggerakkan spermatozoa
• Axoneme – menggerakkan mikrotubulus yang berpola 9+2, sehingga memungkinkan spermatozoa bergerak sliding
• Semua bagian spermatozoa diselubungi membran kecuali pada end piece 14
SOMATIC AND ASOMATIC CELLS
7
BIOLOGI - ACHADIAH RACHMAWATI
Axoneme
15
Axoneme (SEM)
16
SOMATIC AND ASOMATIC CELLS
8
BIOLOGI - ACHADIAH RACHMAWATI
Spermatozoa abnormal
Mitochondria pada leher spermatozoa
17
Seluruh Tubuh Spermatozoa Diselaputi Membran (Perlu Dijaga)
18
SOMATIC AND ASOMATIC CELLS
9
BIOLOGI - ACHADIAH RACHMAWATI
Seluruh Tubuh Spermatozoa Diselaputi Membran (Perlu Dijaga)
19
Spermatozoa manusia
Spermatozoa Tikus
20
SOMATIC AND ASOMATIC CELLS
10
BIOLOGI - ACHADIAH RACHMAWATI
OOSIT (SEL TELUR)
21
22
SOMATIC AND ASOMATIC CELLS
11
BIOLOGI - ACHADIAH RACHMAWATI
23
24
SOMATIC AND ASOMATIC CELLS
12
BIOLOGI - ACHADIAH RACHMAWATI
25
26
SOMATIC AND ASOMATIC CELLS
13
BIOLOGI - ACHADIAH RACHMAWATI
27
OOGENESIS • Mitosis • Meiosis I dan II – Meiosis I (pembentukan oosit primer ke oosit sekunder) – Meiosis II (pembentukan ovum dengan polar bodi)
• Oosit siap untuk fertilisasi pada saat Meosis II (metafase) 28
SOMATIC AND ASOMATIC CELLS
14
BIOLOGI - ACHADIAH RACHMAWATI
Every folliculogenesis step, oocytes undergo atresia and then the best oocytes can be ovulated.
29
Possible time of action of nutrition on folliculogenesis Gr ing ow
-180
Re
-50
-9
Very little atresia
Primordial
d ite u r c
S
-4
c e le
te
d
Ovulation 0
+1
Time (days)
50 - 80% atresia
Small Antral
SOMATIC AND ASOMATIC CELLS
Large antral
+10
Pre-ovulatory
Corpus luteum
30
15
BIOLOGI - ACHADIAH RACHMAWATI
31
Development of Germ Cell Biotechnology Clonning
32
SOMATIC AND ASOMATIC CELLS
16
BIOLOGI - ACHADIAH RACHMAWATI
Development of Germ Cell Biotechnology
33
Embryonic Stem Cell
34
SOMATIC AND ASOMATIC CELLS
17