DAFTAR PUSTAKA. Adnan, M Controvercy About Dietary Fats. Indonesian Food and Nutrition Progress 1 :

DAFTAR PUSTAKA

Adiotomre, .I., M.A. Eastwood, C.A. Edwards and W.G. Brydod. 1990. Dietary fiber : in vitro methods that anticipate nutrition and metabolic activity in humans. Am. J. Clin. Nutr. 52 : 128-134. Adnan, M. 1994. Controvercy About Dietary Fats. Indonesian Food and Nutrition Progress 1 :66-67. Advertorial. 1996. Sehat dengan Biskuit Berserai. Majalah NOVA No. 434/LX, tanggal 16 Juni. Alkanhal, M.A. 1996. Effect of carnitine supplementation on tissue lipid levels and ultrastructural changes in rats fed a diet containing gahwa (Arabian coffee) and cholesterol. Medical Science Research 24 :607-612. Andersen, G.J. and W.E. Connor. 1994. Accretion of n-3 fatty acids in the brain and retina of chicks fed a low-linoleic acid diet supplemented with docosahexaenoic acid. Am. J. Clin. Nu@. 59 : 1338-1346. Aoyama, T., K. Fukui, K. Taniguchi, S. Nagaoka, T. Yamamoto and Y. Hashimoto. 1996. Absorption and Metabolism of Lipids in Rats Depend on Fatty Acid Isomeric Position. J. Nurr. 126 :225-231. Athyros, V.G., A.A. Papageorgiou, M.J. Avramidis, A.J. K a r a y d s , C.X. Zamboulis and A.G. Kontopoulos. 1994. Effect of pravastatin in the prevention of coronary heart disease in patients with primary hypercholesterolemia. Current Therapeutic Research - Clinical and Experimental 55 :914-924. Aye, K.J. and A.J. Hulbert. 1996. Dietary Fatty Acid Profile Influences the Composition of SkeletalMuscle Phospholipids in Rats. J. Nu??-.126 : 653662. Barker, D.L. and J.L. Sell. 1994. Dietary Carnitine Did Not Influence Performance and Carcass Composition of Broiler Chickens and Young Turkeys Fed Lowor High-Fat Diets. Poult. Sci. 73 :281-287. Bartholmey, S.J. and A.R. Sherman. 1986. Postweaning Camitine Supplementation of Iron-Deficient Rats. J. Nutr. 116 :2190-2200.

Bananti, V., P. Pregnolato, M. Maranesi, 1. Bosi, A. Baracea, G. Solaini and E. Turcheno. 1995. Effect of dietary oils containing graded amounts of 18 : 3 n-6 and 18 : 4 n-3 cell plasma membranes. J. Nutr. Biochem. 6 :21-26. Basu, T.K., B. Ooraikul and M.L. Garg. 1993. Effects of dietary' pectin on hte hepatic activities of hydroxymethyl glutaryl. CoA reductase and acyl CoA cholesterol acyl-transferase in cholesterol supplemented mice. J. Nutr. Biochem. 4 :472-475. Bell, F.P., T.J. Vidrnar and T.L. Raymond. 1992. L-Carnitine Administration and Withdrawal Affect Plasma and Hepatic Camitine Concentrations, Plasma Lipid and Lipoprotein Composition, and In Vitro Hepatic Lipogenesis from Labeled Mevalonate and Oleate in Normal Rabbits. J. Nutr. 122 :959-966. Berger, R. and D.S. Sachan. 1991. Effects of supplementary levels of Gcarnitine on blood and urinary carnitines and on the portal-systemic bloodethanol concentrations in the rat. J. Nutr. Biochem. 2 :382-386. Bhuiyan, J. and D.W. Seccombe. 1996. The effects of 3-hydroxy-3-methylglutarylCoA reductase inhibition on tissue levels of carnitine and carnitine acyltransferase activity in the rabbit. Lipids 31 :867-870. Bianchi, P.B. and A.T. Davis. 1991. Sodium Pivalate Treatment Reduced Tissue Carnitines and Enhances Ketosis in Rats. J. Nutr. 121 :2029-2036. Bianchi, P.B. andA.T. Davis. 1996. B-Hydroxybutyrate Oxidation Is Reduced and Hepatic Balance of Ketone Bodies and Free Fatty Acids Is Unaltered in Carnitine-Depleted, Pivalate-Treated Rats. J. Nutr. 126 :2867-2872. Bianchi, P.B., D.C. Lehotay and A.T. Davis. 1996. Carnitine Supplementation Ameliorates the Steatosis and Ketosis Induces by Pivalate in Rats. J Nutr. 126 :2873-2879. Black, D.M. and S.L. Knauer. 1991. The Legal Implications of Dietary Fats : Risks of Cardiovascular Disease and the Duty of Food Manufacturers. J. Nutr. 121 :578-582. Blok, W.L., M.B. Katan and J.W.M. van der Meer. 1996. Modulation on Inflammation and Cytokine Production by Dietary (n-3) Fatty Acids. J. Nutr. 126 : 1515-1533.

Blonk, M.C., H. JG. Bilo, J. JP. Nauta, C. Popp-Snijders, C. Mulder, and Ab. JM. Donker. 1990. Dose-response effects of fish-oil supplementation in healthy volunteers. Am. J, Clin. Nub. 52 : 120-127. Bonaa, K.H., K.S. Bjerve and A. Nordoy. 1992. Habitual fish constmption, plasma phospholipid fatty acids, and serum lipids : the Tromso Study. Am. J. Clin. Nutr. 55 :1126-1134. Bonanome, A., F. Biasia, M. De Luca, G. Munareno, S. Biffanti, M. Pradella and A. Pagnan. 1996. n-3 Fatty acids do not enhance LDL susceptibility to oxidation in hypertriacylglycerolemic hemodialyzed subjects. Am. J. Clin. Nutr. 63 :261-266. Brady, L.J., P.S. Brady, L. Albers, A.T. Davis and C.L. Hoppel. 1986. Camitine Metabolism in Lean and Obese Zucker Rats during Starvation. J. Nutr. 116

.' 668-674. Brady, L.J., R.R. Rarnsay and P.S. Brady. 1991. Regulation of Carnitine Acyltransferase Synthesis in Lean and Obese Zucker Rats by Dehydroepiondrosteroneand Clofibrate. J. Nutr. 121 :525-531. Brass, E.P.and L.J. Rdf. 1989. Effect of Carnitine on Propionate Metabolism in the Vitamin B-12- Deficient Rat. J. Nutr. 119 :1196-1202. Brooks, S.D., J.J. Bahl and R. Bressler. 1985. Camitine in the Streptozotocin : Diabetic Rat. J. Nutr. 115 :1267-1273. Broughton, K.S., J. Whelan, I. Hardardottir and J.E. Kinsella. 1991. Effect of Increasing the Dietary (n-3) to (n-6) Polyunsaturated Fatty Acid Ratio on Murine Liver and Peritoneal Cell Fatty Acids and Eicosanoid Formation. J. Nurr. 121 : 155-164. Broughton, K.S. and L.J. Morgan. 1994. Frequency of (n-3) Polyunsaturated Fatty Acid Consumption Induced Alterations in Tissue Lipid Composition and Eicosanoid Synthesis in CD-I Mice. J. Nutr. 124 : 1104-1111. Burns, C.P. and A.A. Spector. 1994. Biochemical effects of lipids on cancer therapy. J. Nutr. Biochem. 5 : 114-123. Caughey, G.E.,E. Mantzioris, R.A. Gibson, L.G.Cleland and M.J. James. 1996. The effect on human tumor necrosis factor a and interleukin 1 P production of diets enriched in n-3 fatty acids from vegetable oil or fish oil. Am. J. Clin. Nutr. 63 : 116-122.

Cha, M.C. and P.J.H. Jones. 1996. Tissue fatty acid deposition is influenced by an interaction of dietary oil source and energy intake level in rats. J. Nutr. Biochem. 7 :650-658. Chanmugan, P., M. Boudreau, T. Boutte, R.S. Park, 3. Hebert, L.#Berio and D.H. Hwang. 1992. Incorporation of Different Types of n-3 Fatty Acids Into Tissue Lipids of Poultry. Poult. Sci. 71 : 516-521. Chatzifotis, S., T. Takeuchi and T. Seikai. 1996. The effect of dietary carnitine supplementation on growth of red sea bream (Pagrus major) finger-lings at two levels of dietary lysine. Aquaculture 147 : 235-248. Cherian, G. and J. Sim. 1992. Preferential Accumulation of n-3 Fatty Acids in the Brain of Chicks ffom Eggs Enriched with n-3 Fatty Acids. Poult. Sci. 71 :1658-1668.

Cherian, G. and J.S. Sim. 1993. Net Transfer and Incorporation of Yolk n-3 Fatty Acids into Developing Chick Embryos. Poult. Sci 72 :98-105. Cho, Y-0. and JE. Leklem. 1990. In Vivo Evidence for a Vitamin B-6 Requirement in Camitine Synthesis. J. Nutr. 120 :258-265. Clouet, P., I. Niot, J. Gresti, J. Demarquoy, J. Boichot, G. Durand and J. Bezard. 1995. ~ol~unsaturated n-3 and n-6 fatty acids at a low level in the diet alter mitochondrial outer membrane parameters in Wistar rat liver. J. Nutr. Biochem. 6 :626-634. Coffey, M.T.,R.B. Shireman, D.L. Herman and E.E. Jones. 1991. Carnitine status and Lipid Utilization in Neonatal Piglets Fed Diets in Carnitine. J. Nutr. 121 :1047-1053.

Connor, W.E. 1996. The decisive of diet on the progression and reversibility of coronary heart disease. Am. J. Clin. Nutr. 64 :253-254. Contacos, S., P.J. Barter and D.R. Sullivan. 1993. Effect of Pravastatin and omega-3 Fatty Acids on Plasma Lipids and Lipoproteins in Patients with Combined Hyperlipidemia. Arteriosclerosis and Thrombosis 13 : 17551762.

Cunnane, S.C. and M.J. Anderson. 1997. The Majority of Dietary Linoleate in Growing Rats Is P-Oxidized or Stored in Visceral Fat. J. Nutr. 127 : 146152.

Daily Ill, J.W. and D.S. Sachan. 1995. Choline Supplementation Alters Camitine Homeostasis in Humans and Guinea Pigs. J. Nulr. 125 : 1938-1944. Davis, A.T. 1990. Tissue Trimethyllysine Biosynthesis and Carnitine Content in Pregnant and Lactating Rats Fed a Lysine-Limiting Diet. J. Nutr. 120 : 846-856. Davis, A.T., E.M. Kruggel and S. Randall. 1993. Excess Dietary Lysine Increases Skeletal Muscle and Plasma Trimethyllysine in Rats. J. Nutr. 123 :11091116. Deuchi, K., 0. Kanauchi, Y. Imasato and E. Kobayashi. 1994. Decreasing Effect of Chitosan on the Apparent Fat Digestibility by Rats Fed on a High-fat Diet. Biosci. Biotech. Biochem. 58 : 1613-1616. Dichtenberg, J.B., A. Pronczuk and K.C. Hayes. 1995. Hyperlipidemic effects of trans fatty acids are accentuated by dietary cholesterol in gerbils. J. Nutr. Biochem. 6 :353-361. Dietschy, J.M. and H.J. Weis. 1971. Cholesterol synthesis by the gastrointestinal tract. Am. J. Clin. Nuir. 24 :70-76. Enser, M. 1984. The Chemistry, Biochemistry and Nutritional Importance of Animal Fats in Fats in Animal Nutrition Ed. J. Wiseman. Butterworths, London. Eritsland, J., H. Amesen, I. Seljeflot and A.T. Hostmark. 1994. Long-term metabolic effects of n-3 polyunsaturated fatty acids in patients with coronary artery disease. Am. J. Clin. Nutr. 61 : 831-836. Estiasih, T. 1996. Mikroenkapsulasi Konsentrat Asam Lemak Omega-3 dari L i b a h Cair Pengalengan Ikan Lemuru (Surdinella longiceps). Tesis Program Pascasarjana UGM. Yogyakarta. Evans, A.J., R.L. Hood, D.G. Oakenfull and G.S. Sidhu. 1992. Relationship between structure and function of dietary fibre : a comparative study of the effects of three galactomannans on cholesterol metabolism in the rat. Br. J. Nutr. 68 :21 7-229. Felgines, C., A. Mazur and Y. Rayssiguier. 1994. Plasma lipoproteins and apolipoproteins B and E in rats fed a high-fiber fiuctose-based diet. J. Nutr. Biochem. 5 :499-503.

Feller, A.G. and D. Rudman. 1988. Role of Carnitine in Human Nutrition. J. Nutr. 118 :541-547. Femandez, M.L. and D.J. Mc.Namara. 1991. Regulation of Cholesterol and Lipoprotein Metabolism in Guinea Pigs Mediated by Dietary Fat Quality and Quantity. J. Nutr. 121 :934-943. Ferrier, L.K., L.J. Caston, S. Leeson, J. Squires, B.J. Weaver and B.J. Holub. 1995. aLiioleic acid and docosahexaenoic acid-enriched eggs ftom hens fed flaxseed : influenced on blood lipids and plateled phospholipid acid in humans. Am. J. Clin. Nutr. 62 :81-86. Flores, C.A., C. Hu, J. Edmond and 0. Koldovsky. 1996. Milk Carnitine Affects Organ Carnitine Concentration in Newborn Rats. J. Nutr. 126 :1673-1682. Fritsche, K.L. and N.A. Cassity. 1992. Dietary n-3 Fatty Acids Reduce AntibodyDependent Cell Cyto toxicity and Alter Eicosanoid Release by Chicken Immune Cells. Poult. Sci. 71 : 1646-1657. ~altaher,D.D., C.A. Hassel and K-J Lee. 1993. Relationships between Viscosity of Hydroxypropyl Methylcellulose and Plasma Cholesterol in Hamsters. J. Nu*. 123 :1732-1 738. Garcia-Diez, F., V. Garcia-Mediavilla, J.E. Bayond and J. Gonzales-Gallego. 1996. Pectin Feeding Influences Fecal Bile Acid Excretion, Hepatic Bile Acid and Cholesterol Synthesis and Serum Cholesterol in Rats. J. Nutr. 126 :' 1766-1 771. Gaudette, D.C. and B.J. Holub. 1991. Docosahexaenoic acid @HA) and human platelet reactivity. J. Nufr. Biochem. 2 : 116-121. Gey, K.F. 1995. Ten-year retrospective on the antioxidant hypothesis of atherosclerosis : Threshold plasma levels of antioxidant micronutrients related to minimum cardiovascular risk. J. Nufr. Biochem. 6 :206-236. Ghosh, P., M. Somanchi and M.R. Lakshman. 1995. Regulatory enzymes of lipid metabolism in LAM-cp rats. J. Nub. Biochem. 6 :348-352. Grundy, S.M. 1989. Monounsaturated Fatty Acids and Cholesterol Metabolism : implications for Dietary Recommendations. J. Nutr. 119 :529-533. Ha, T.Y.,M. Otsuka and N. Arakawa. 1994. Ascorbate Indirectly Stimulates Fatty Acid Utilization in Primary Cultured Guinea Pig Hepatocytes by Enhancing Camitine Synthesis. J. Nutr. 124 : 732-737.

Hamazaki, T., S. Sawazaki, E. Asaoka, M. Itomura, Y. Mizushima, K. Yawam, T. Kuwamori and M. Kobayashi. 1996. Docosahexaenoic Acid-Rich Fish Oil Does Not Affect Serum Lipid Concentrations of Normolipidemic Young Adults. J. Nutr. 126 :2784-2789. L

-

Hanschrnann, H, and H.P. Kleber. 1997. Purification .and characterization of D (+) carnithe dehydrogenase from Agrobacterium sp. A new enzyme of camitine metabolism. Biochimica et Biophysica Acra - Protein Structure and Molecular Enzymology 1337 : 133-142.

-

Harper, P.C., Waldstroem. L. Backman and G. Cederblad. 1995. Increased liver camitine content in obese women. Am. J. Clin.Nutr. 61 :18-25.

Haumann, B.E., 1996. Conjugated Lioleic acid offers research promise. INFORM 7 : 152-159. Hayes, K.C., A. Pronczuk and P. Khosla 1995. A rationale for plasma cholesterol modulation by dietary fatty acids : Modeling the human response in animals. J Nutr. Biochem. 6 :188-194. Hibbeln, J.R. and N. Salem Jr. 1995. Dietary polyunsaturated fatty acids and depression : when cholesterol does not satisfy. Am. J. Clin. Nutr. 62 :1-9. Hicks, V., S.C. Chen, S.A. Tepper and D. Kritchevsky. 1995. The cholesterollowering effect of oat bran cereals in rats : Muence of processing. J. Nutr. Biochem. 6 :246-249. H i , A. and T.A. B. Sanders. 1993. The effect of increasing level of dietary fish oil rich in eicosapentaenoic and docosahexaenoic acids on lymphocyte phospholipid fatty acid composition and cell-mediated immunity in the mouse. Br. J Nutr. 69 :423-429.

Hodgson, J.M., M.L. Wahlquist, J.A. Boxall and N.D. Balazs. 1993. Can linoleic acid contribute to coronary artery disease ?Am. J. Clin. Nutr. 58 : 228-234. Honeyfield, D.C. and J.A. Froseth. 1991. Evaluation of Energy Sources With and Without Carnitine in Newborn Pig Heart and Liver. J. Nutr. 121 : 1117-1122. Horigome, T., E. Sakaguchi and C. Kishimoto. 1992. Hypocholesterolaemic effect of banana (Mura sapientum L. var. Cavendishii) pulp in the rat fed on a cholesterol-containing diet. Br. J. Nutr. 68 :231-244.

Howard, B.V., J.S. Hannah, C.C. Heiser, K.A. Jablonski, M.C. Paidi, L. Alarif, D.C. Robbins and W.J. Howard. 1995. Polyunsaturated fatty acids results in greater cholesterol lowering and less triacylglycerol elevation than do monounsaturated fatty acids in a dose-response comparison in a multiracial study group. Am. J. Clin. Nutr. 62 : 392-402.

.

Hrboticky, N., B. Zimrner and P.C. Weber. 1996. a-Linolenic acid reduces the lovastatin-induced rise in arachidonic acid and elevated cellular and lipoprotein eicosapentaenoic and docosahexaenoic acid levels in Hep G2 cells. J. Nurr. Biochem. 7 :465-471. Hudyono, J. and D.S. Raharja. 1995. Gambaran Kadar Gula Darah clan Kolesterol Pejabat Struktural UKRIDA. MEDITEK 3 :45-60. Hundemer, J.K., S.P. Nabar, B.J. Shriver and L.P. Forman. 1991. Dietary Fiber Sources Lower Blood Cholesterol in C 57 BL/6 Mice. J. Nutr. 121 : 1360-1365. Hunninghake, D.B., V.T. Miller, Y.C.LaRosa, B. Kinosian, V. Brown, W.J. Howard, F.J. DiSerio and R.R. O'Connor. 1994. Hypocholesterolemic effects of a dietary fiber supplement. Am. J. Clin.Nuir. 59 :1050-1054.

Igal, A. and N.T. de Gomez Dumm. 1995. Influence of dietary n-3 fatty acids on the biosynthesis of polyunsaturated fatty acids in STZ-diabetic rats. J Nutr. Biochem. 6 :269-274.

Ikeda, I., Y. Tomari and M. Sugano. 1989. Interrelated Effects of Dietary Fiber and Fat on Lymphatic Cholesterol and Triglyceride Absorption in Rats. J. Nutr. 119 :1383-1387. Irnaizumi, K., K. Abe, C. Kuroiwa and M. Sugano. 1993. Fat Containing Stearic Acid Increases Fecal Neutral Steroid Excretion and Catabolism of Low Density Lipoproteins Without Affecting Plasma Cholesterol Concentration in Hamsters Fed a Cholesterol-Containing Diet. J. Nutr. 123 : 1693-1702. Indrati, N. 1995. Pola Makan Sehat sebagai Usaha Pencegahan Serangan Stroke dan Diabetes. Duta Kampus Borobudur Nomor 8 : 157-167. Innis, S.M., F.M. Rioux, N. Auestad and R.G. Ackman. 1995. Marine and Freshwater Fish Oil Varying in Arachidonic, Eicosapentaenoic and Docosahexaenoic Acids Differ in their Effects on Organ Lipids and Fatty Acids in Growing Rats. J. Nutr. 125 : 2286-2293.

Ismadi, M. 1993. Biokimia. Suatu pendekatan berorientasi-kasus. Jilid 2. Edisi Keempat, Gadjah Mada University Press. Ismail, E.Y., H.M. Teleb and O.E.Mohamed. 1989. Dry Kemzyme brand enzyme and laying hen performance. Kemin Industries, Inc. publitation no. 07229, pp. 1 4.

-

Itaya, S. and K. Igarashi. 1992. Effects of Taxifolin on the Serum Cholesterol Level in Rats. Biosci. Biotech. Biochem. 56 :1492-1494. Jandacek, R.J., E.J. Hollenbach, B.N. Holcombe, C.M. Kuehlthau, J.C. Peters and J.D. Taulbee. 1991. Reduced storage of dietary eicosapentaenoic and docosahexaenoic acids in the weanling rat. J. Nuir. Biochem. 2 : 142149. Jeffery, N.M., P. Yaqoob, D. Wiggins, G.F. Gibbons, E.A. Newsholme and P.C. Calder. 1996. Characterization of lipoprotein composition in rats fed different dietary lipids and of the effects of lipoproteins upon lymphocyte proliferation. J. Nu*. Biochem. 7 :282-292. Ji, H., T.M. Bradley and G.C. Tremblay. 1996. Atlantic Salmon (Salmo solar) Fed GCarnitine Exhibit Altered Intermediary Metabolism and Reduced Tissue Lipid, but No Change in Growth Rate. J. Nuh: 126 : 1937-1950. Jolly, C.A., Y.-H. Jiang, R.S. Chapkin and D.N. McMuny. 1997. Dietary (n-3) Polyunsaturated Fatty Acids Suppress Murine Lymphoproliferation, Interleukin-2 Secretion, and the Formation of Diacylglycerol and Ceramide. J. Nuir. 127 :37-43. Jonnalagadda, S.S., F.W. Thye and J.L. Robertson. 1993. Plasma Total and Lipoprotein Cholesterol, Liver Cholesterol and Fecal Cholesterol Excretion in Hamsters Fed Fiber Diets. J. Nutr. 123 : 1377-1382. Karninska, J., K.A. Nalecz, A. Azzi and M.J. Nalecz. 1993. Purification of Carnitine Canier from Rat Brain Mitochondria. Biochemistry and Molecular Biology International 29 :999-1007. Keidar, S., M. Aviram, 1. Maor, J. Oiknine and J.G. Brook. 1994. Pravastatin inhibits cellular cholesterol synthesis and increases low density lipoprotein receptor activity in macrophages : In vitro and in vivo studies. British Journal of Clinical Pharmacology 38 :513-519. Kelley. D.S. 1996. Dietary fat and human immune response. INFORM 7 :852-858.

Kempen, T.A.T.G. and J. Odle. 1995. Carnitine Affects Actanoate Oxidation to Carbon Dioxide and Dicarboxylic Acids in Colostrum - Deprived Piglets : In Vivo Analysis of Mechanisms Involved Based on CoA and Carnitine Ester Profiles. J. Nutr. 125 :238-250.

-

,

Kempen, T.A.T.G. and J. Odle. 1993. Medium-Chain Fatty Acid Oxidation in Colostrum-Deprived Newborn Piglets : Stimulative Effect of L Carnitine Supplementation. J. Nutr. 123 : 1531-153 7. Khokhar, S. 1994. Dietary fibers : their effects on intestinal digestive enzyme activities. . INub. Biochem. 5 : 176-180. Kingrnan, S.M., A.F. Walker, A.G. Low, IE. Sarnbrook, R.W. Owen and T.J. Cole. 1993. Comparative effects of four legume species on plasma lipids and faecal steroid excretion in hypercholesterolemic pigs. Br. J. Nutr. 69 : 409421. Kisella, J.E., B. Lokesh and R.A. Stone. 1990. Dietary n-3 polyunsaturated fatty acids and amelioration of cardiovascular disease : possible mechanisms. Am. 1 Clin. Nu& 52 :1-28. Klurfeld, D.M. 1995. Fat effects in experimental tumorigenesis. . INuti-. Biochem. 6 : 201-205. Knudsen, K.E.B., E. Wisker, M. Daniel, W. Feldheim and B.O. Eggum. 1994. Digestibility of energy, protein, fat and non-starch polysaccharides in mixed diets : comparative studies between man and the rat. Br. J. Nuti-. 71 :471487. Komari. 1994. Mienkapsulasi Minyak Ikan untuk Fortifikasi Asam Lemak Omega-3 ke &lam Makanan. Gizi Indonesia - Journal of the Indonesian Nutrition Association 19 (1 -2) :90-100. Kritchevsky, D. and S.A. Tepper. 1995. Influence of dietary fiber on establishment and progression of atherosclerosis in rabbits. J. Nuh: Biochem. 6 : 509512. Kritchevsky, D. 1995. Fatty acids, triglyceride structure, and lipid metabolism. J. Nufr. Biochem. 6 :172-178. Layne, K.S., Y.K.Goh, J.A. Jurnpsen, E.A. Ryan, P. Chow and M.T. Clandinin. 1996. Normal Subjects Consuming Physiological Levels of 18 : 3 (n-3) and 20 : 5 (n-3) from Flaxseed or Fish Oils Have Characteristic Defferences in Plasma Lipid and Lipoprotein Fatty Acid Levels. J. Nufr. 126 :2130-2140.

Leaf, D.A., W.E. Connor, L. Barstad and G. Sexton. 1995. Incorporation of dietary n-3 fatty acids into the fatty acids of human adipose tissue and plasma lipid classes. Am. J. Clin. Nutr. 62 :68-73. Leibetseder, J. 1995. Studies of Lcarnitine effects in poultry. Archives of Animal Nutrition Archiv fuer Tierernaehrung 48 :97-108.

-

Leray, C., M. Andriamampandry, G. Gutbier. T. Raclot and R. Groscolas. 1995. Incorporation of n-3 fatty acids into phospholipids of rat liver and white I and brown adipose tissues: A time-course study during fish-oil feeding. . Nutr. Biochem. 6 : 673-680. Lessard, P., M.R. L e h c o i s and J.F. Bemier. 1993. Dietary Addition of Cellular Metabolic Intermediates and Carcass Fat Deposition in Broilers. Poult. Sci. 72 :535-545. Li, BUK., M.L. Lloyd, H. Gudjonsson, A.L. Shug and W.A. Olsen. 1992. The effect of enteral camitine administration in humans. Am. J. Clin. Nun: 55 : 838845. Liepa, G.U. and M.A. Gorman. 1991. Nutritional and Health Aspects of Dietary Lipids in Introduction to Fats and Oils Technology, Edited by P.J. Wan. American Oil Chemists' Society, Champaign, Illinois.

Li,C.Y. and D.W. Morel. 1995. Distribution of oxysterols in human serum

:

Characterization of 25-hydroxy-cholesterol association with semh albumin. J. Nub-. Biochem. 6 :618-1525, Lin, D.S. and W.E. Connor. 1990. Are the n-3 fatty acids &om dietary fish oil deposited in the triglyceride stores of adipose tissue ? Am. J. Clin. Nub-. 51 :535-539. Lombard, K.A., A.L. Olson, S.E. Nelson, and C.J. Rebouche. 1989. Carnitine status of iactoovovegetarians and strict vegetarian adults and children. Am. J. Clin. Nutr. 50 0: 301-306.

Lu, S.C.,

M-H. Lin and P-C. Huang. 1996. A High Cholesterol, (n-3) Polyunsaturated Fatty Acid Diet Induces Hypercholesterolemia More Than a High Cholesterol, (n-6) Polyunsaturated Fatty Acid Diet in Hamster. J. Nurr. 126 : 1759-1765.

Luo, J., S.W. Rizkalla, J. Boillot, C. Alamowitch, H. Chaib, F. B ~ z z o ,N. Desplanque. A-M. Dalix, G. Durand and G. Slama. 1996. Dietary (n-3) and Polyunsaturated Fatty Acids lmprove Adipocyte lnsulin Action Glucose Metabolism in Insulin-Resistant Rats : Relation to Membrane L Fatty Acids. J Nutr. 126 :1951-1958. Maezaki, Y., K. Tsuji, Y. Nakagawa, Y. Kawai, M. Akimoto, T. Tsugita, W. Takekawa, A. Terada, H. Hara and T. Mitsuoka. 1993. Hypocholesterolemic Effect of Chitosan in Adult Males. Biosci. Biotech. Biochem. 57 :1439-1444. Marignani, P.A. and R.J. Sebaldt. 1995. Formation of Second Messenger DiradylglyceroI in Murine Peritoneal Macrophages Is Altered after In Vivo (n-3) Polyunsaturated Fatty Acid Supplementation. J Nutr. 125 : 30303040.

Marshall, A.C., K.S. Kubena, K.R. Hiton, P.S. Hargis and M.E. Van Elswyk. 1994. n-3 Fatty Acid Enriched Table Eggs : A Survey of Consumer Acceptability. Poult. Sci 73. :1334-1340. Martin, D.W.Jr., P A . Mayes and V.W. Rodwell. 1984. Biochemishy Harper's Review of Biochemistry. 19th ed. Lange Medical Publ., Los Altos, California. Martinez, V.M., R.K. Newman and C.W. Newman. 1992. Barley Diets with Different Fat Sources have Hypocholesterolemic Effects in Chicks. J.' Nu&. 122 :1070-1076. Matheson, H.B., I.S. Colon and J.A. Story. 1995. Cholesterol 'la-Hydroxylase Activity Is Increased by Dietary Modification with Psyllium Hydrocolloid, Pectin, Cholesterol and Cholestyramine in Rats. J Nuh.125 :454-458. Matsunaga, A., J. Sasaki, K. Hidaka and Y. Takada. 1994. Pravastatin-induced changes in receptor - mediated metabolism of low density lipoprotein in guinea pigs. Artery 21 :94-113. Michalak, A. and 1.A. Qureshi. 1990. Plasma and Urinary Levels of Hyperammonernia and the Effect of Sodium Benzoate Treatment. Biochemical Medicine and Metabolic Biology 43 :163-1 74. Moundras, C., C. Demigne, A. Mazur and C. Remesy. 1995. The cholesterol lowering effect of steroid sequestrants is modulated by large intestine fermentations. J. Nutr. Biochem. 6 :158-162.

Muhilal, S. Herman and D. Karyadi. 1994. Composition of Omega-6 and Omega-3 Fatty Acid in Breastmilk and Infant Formula. Gizi lndonesia - Journal of the Indonesian Nutrition Association 19 (1-2) : 65-68. N.R.C. 1994. Nutrient Requirements of Poultry. Ninth Revised Edition, National Academy Press, Washington, D.C. Nakajima, H., N. Kodo, F. Inoue, E. Kizaki, S. Nukina, A. Kinugasa and T. Sawada. 1996. Pivalate Affects Carnitine Status but Causes No Severe Metabolic Changes in Rat Liver. J. Nutr. 126 :1683-1687. Nalecz, K.A. and M.J. Nalecz. 1993. Transport and metabolic effects of carnitine and acylcarnitines in brain. Acta Biochimica Polonica 40 :337-343. Nardiii, M., M.D. Aquino, G. Tomassi, V. Gentili, M.D. Felice and C. Scaccini. 1995. Dietary fish oil enhances plasma and LDL oxidative modification in rats. J. Nutr. Biochem. 6 :474-480. Nelson, C.E. 1989. Maximizing Animal Productivity Through Dry Kemzyme Brand Enzyme. Kemin Industries, Inc. U.S.A. publication no. 02236, pp. 112. Nestel, P.J., M. Noakes, G.B. Belling, R McArthur and P.M. Clifton. 1995. Effect on plasma lipids of interesterifying a mix of edible oils. Am. J. Clin. Nub. 62 :950-955. Newton, I.S. 1996. Food enrichment with long-chain n-3 PUFA. IrNFORM 7 : 169177. Nishida, N., T. Sugimoto, T. Takeuchi and Y. Kobayashi. 1989. Effect of L-Carnitine on GLycogen Synthesis and ATP Production in Cultured Hepatocytes of the Newborn Rat. J. Nu@. 119 : 1705-1708. Nishina, P.M. and R.A. Freedland. 1990. The Effects of Dietary Fiber Feeding on Cholesterol Metabolism in Rats. J. Nutr. 120 :800-805. Noy, Y. and D. Sklan. 1995. Digestion and Absorption in the Young Chick. Poult. Sci. 74 :366-373. Ogawa, T., M. Gatchalian-Yee, M. Sugano, M. Kimoto, T. Matsuo and Y. Hashimoto. 1992. Hypocholesterolemic Effect of Undigested Fraction of Soybean Protein in Rats Fed No Cholesterol. Biosci. Biotech. Biochem. 56 : 1845-1848.

Opara, E.C. and V.S. Hubbard. 1993. Essential faay acids (EFA) : Role in pancreatic hormone release and concomitant metabolic effect. J. Nutr. Biochem. 4 :498-509. Osada, K., E. Sasaki and M. Sugano. 1994a. Inhibition of Cholestefol Absorption by Oxidized Cholesterol in Rats. Biosci. Biotech..Biochem. 58 : 782-783. Osada, K., T. Kodama, L. Cui, Y. Ito and M. Sugano. 1994b. Effect of Dietary Oxidized Cholesterol on Lipid Metabolism in Differently Aged Rats. Biosci. Biotech. Biochem. 58 :1062-1069. Overton, P.D., N. Furlonger, J.M. Beety, J. Chakraborty, J.A. Tredger and L.M. Morgan. 1994. The effects of dietary sugar-beet fib* and guar gum on lipid metabolism in Wistar rats. Br. J. Nutr. 72 :385-395. Paniangvait, P., A.J. King, A.D. Jones and B.G. German. 1995. Cholesterol Oxides in Foods of Animal Origin. J. Food. Sci 60 :1159-11 74. Pasquier, B., M. Armand, F. Guillon, C. Castelain, P. Borel, J-L. Bany, G. Pieroni and D. Lairon. 1996. Viscous soluble dietary fibers alter emulsification and lipolysis of triacylglycerols in duodenal medium in vitro. J. Nutr. Biochem. 7 : 292-302. Paul, H.S. and G. Sekas. 1992. Hepatic Release of Carnitine : Effect of Increased Concentration by Clofibrate Treatment. Biochemical Medicine and Metabolic Biology 47 :21-30. Paulson, D.J., G.E. Hoganson, J. Traxler, R. Sufit, H. Peters and A.L. Shug. 1988. Ketogenic Effects of Carnitine in Patients with Muscular Dystrophy and Cytochrome Oxidase Deficiency. Biochemical Medicine and Metabolic Biology 39 :40-47. Pearson, D.J. and P.K. Tubbs. 1967. Carnitine and Derivatives in Rat Tissues. Biochem. J. 105 : 953-963. Peck, M.D. 1994. Interaction of lipids with immune function 1 : Biochemical effects of dietary lipids on plasma membranes. J. Nutr. Biochem. 5 : 466478. Phetteplace, H.W. and B.A. Watkins. 1992. influence of Dietary n-6 and n-3 Polyunsaturated on Lipids in Chickens Divergently Selected for Body Weight. Poult. Sci. 71 :1513-1519.

Phinney, S.D., R.S. Odin, S.B. Johnson and R.T. Holman. 1990. Reduced arachidonate in serum phospholipids and choleste~ylesters associated with vegetarian diets in humans. Am. J. Clin. Nutr. 51 :385-392. Pierpont, M.E.M., D.B. Judd. D.P. Tukey and C.M. Smith ll. 1988. Erythrocyte Carnitine : A Study on Erythrocyte Fractipns Isolated by Discontinous Density Gradient Centrifugation. Biochemical Medicine and Metabolic Biology 40 :237-246. Pinchasov, Y. and I. Nir. 1992. Effect of Dietary Polyunsaturated Fatty Acid Concentration of Performance, Fat Deposition, and Carcass Fatty Acid Composition in Broiler Chickens. Poult. Sci. 71 : 1504-1512. Pryde, E.H. 1985. Natural Fatty Acids and Their Sources in Fatty Acids. Edited by E.H.Pryde. The American Oil Chemists' Society, Champaign, Illinois. Qureshi, A.A., J.W. Lehmann and D.M. Peterson. 1996. Amaranth and Its Oil Inhibit Cholesterol Biosynthesis in 6-Week-Old Female Chickens. J. Nutr. 126 :1972-1978.

Raharjo. S. 1995. Produk Oksidasi Lemak Salah Satu Penyebab Penyakit Jantung Koroner. Agritech. 15 :31-35.

Razdan, A. and D. Pettersson. 1994a. Effects of feeding restriction and meal pattem of a sugar beet-containing diet and control diet on nutrient digestibility, plasma lipid concentrations and postprandial triacylglycerol response in broiler chickens. Br. J. Nutr. 71 :389-400. Razdan, A. and D. Pettersson. 1994b. Effect of chitin and chitosan on nutrient digestibility and plasma lipid concentrations in broiler chickens. Br. J. Nutr. 72 :2 77-288. t

Rebouche, C.J. 1983. Effect of Dietary Carnitine Isomers and YButyobetaine on LCarnitine Biosynthesis and Metabolism in the Rat. J. Nutr. 113 : 1906-1913. Rebouche, C.J. and C.A. Chenard. 1991. Metabolic Fate of Dietary Carnitine in Human Adults : Identification and Quantification of Urinary and Fecal Metabolites. J. Nutr. 121 :539-546. Rebouche, C.J., D.L. Mack and P.F. Edmonson. 1984. L-Camitine Dissimilation in the Gastrointestinal Tract of the Rat. Biochemistry 23 :6422-6426.

Rebouche, C.J., K.A. Lombard and C.A. Chenard. 1993. Renal adaptation to dietary camitine in humans. Am. J. Clin. Nutr. 58 :660-665. Rebouche, C.J. 1986. Is Carnitine an Essential Nutrient for Humans ? J. Nutr. 116 : 704-706. , Rebouche, C.J., E.P. Bosch, C.A. Chenard, K.J. ~chaboldand S.E. Nelson. 1989. Utilization of Dietary Precursors for Carnitine Synthesis in Human Adult. J. Nue. 119 : 1907-1913. Rebouche, C.J., L.J. Lehman and A.L. Olson. 1986. E-N-Trimethyllysine Availability Regulates the Rate of Carnitine Biosynthesis in the Growing Rat. J. Nutr. 116 :751-759. Reddy, B.S. 1994. Chemoprevention of colon cancer by dietary fatty acids. Cancer and Metastasis Reviews 13 :285-302. Reid, B.L. 1984. Enzymes and laying hen performance. Report January 5, pp. 1-22.

Rhew, T.H. and D.S. Sachan. 1986. DoseDependent Lipotropic Effect of Carnitine in Chronic Alcoholic Rats.J. Nutr. 116 :2263-2269. Rolow, A.M. and C.E. Nelson. 1987. Enzyme supplementation of growing and fmishing swine rations. Kemin Industries. Inc. publication no. 02237, pp. 1-3. Sachan, D.S. and R.A. Ruark. 1985. Distribution of Carnitine and Acylcarnitine in Small Intestine of Carnitine-Supplemented and Fasted Rats. J. Nutr. 115 : 865-871. Santoso, U. 1991. Studi tentang Kitin Cangkang Udang Il : Hidrolisis dengan Lisozim. Agrirech l l : 14-20. Sardesai, V.M. 1992a. Nutritional role of polyunsaturated fatty acids. J. Nutr. Biochem. 3 :154-166. Sardesai, V.M. 1992b. Biochemical and nutritional aspects of eicosanoids. J. Nutr. Biochem. 3 : 562-579. SASISTAT. 1988. SAS/STAT User's Guide: Statistics. Release 6.03 Edition. SAS Institute lnc., Cary, NC, USA.

Sato. M.. T. Nakano, M. Takeuchi, N. Kanno, E. Nagahisa, Y. Sato and Y. Kobatake. 1996. Effects of Octopine on Serum Cholesterol Level in Rats. Biosci. Biotech. Biochem. 60 :154-156. Scaife, J.R., J. Moyo, H. Galbraith, W. Michie and V. Campbell. ,1994. Effect of different dietary supplemental fats and oils on the tissue fatty acid composition and growth of female broilers. Br. Poult. Sci. 35 : 107-118. Schaefer, E.J., A.H. Lichtenstein, S. Lamon-Fava, J.H.Contois, Z. Li. B.R. Goldin, H. Rasmussen, J.R.McNamara and J.M. Ordovas. 1996. Effects of National Cholesterol Education Program Step 2 diets relatively high or relatively low in fish-derived fatty acids on plasma lipoproteins in middle-aged and elderly subjects. Am. J. Clin. Nuir. 63 :234-241. Schaefer, E.J., A.H. Lichtenstein, S. Larnon-Fava, J.R. McNamara and J.M. Ordovas. 1995. Lipoproteins, nutrition, aging, and atherosclerosis. Am. J. Clin. Nutr. 61 :726 S-740 S. Schectman, G., L.E. Boerboom, J. Hannah, B.V., Howard, R.A. Mueller and A.H. Kissebah. 1996. Dietary fish oil decreases low-density-lipoprotein clearance in nonhuman primates. Am. J. Clin. Nu@. 64 :215 - 221. Seidelin, K.N., B. Myrup and B. Fischer-Hansen. 1992. n-3 fatty acids in adipose tissue and coronary artery disease are inversely related. Am. J. Clin. Nutr. 55 : 1117-1119. Sheard, N.F. and B. Krasin. 1994. Restricting Food Intake Does Not Exacerbate the Effects of a Cholme-Deficient Diet on Tissue Carnitine Concentrations in Rats. . I Nuir. 124 :738-743. Shiomi, M., Shiraishi, T. Yata and T. Ito. 1994. Effect of fluvastatin sodium on secretion of very low density lipoprotein and serum cholesterol levels In vivo study using low density lipoprotein receptor deficient Watanabe heritable hyperlipidemic rabbits. Anneimittel Forschung/Drug Research. 44 : 1154-1156.

-

Shrapnel, B. 1995. Wheat fibre and bowel cancer. Perspectives in Food and Nutrition. Issue one Spring. pp. 9-11. Simopoulos, A.P. 3989. Summary of the NATO Advanced Research Workshop Fatty Acids : Biological Effects and Nutritional on Dietary and (06 Essentiality. J. Nutr. 119 :521-528.

Sinclair, A. 1996. Does fish prevent heart disease ? Perspectives in Food and Nutrition. Issue three Autumn. pp. 1-12. Soepamo, 1994. Sadar Gizi, Penerapan llmu dan Teknologi dalam lndustri Daging. Pidato Pengukuhan Jabatan Guru Besar dalam Ilmu Teknobgi Pengolahan Daging pada Fakultas Peternakan UGM 29 Desember 1994. Soepamo. 1995. Ilmu clan Teknologi : Aspek Daging Unggas dan Penanganannya. Pembukaan Kuliah Program Studi Ilmu Petemakan, Program Pascasarjana UGM tanggal 9 September 1995. Soerjodibroto, W. 1995. Semua Jenis Minyak Goreng Berbahaya Terutama bagi Penderita Hiperlipidemia. Harian KOMPAS, Minggu 8 Januari. Suarez, A., M.D.C. Ramires, M.J. Faus and A. Gil. 1996. Dietary Long-Chain Polyunsaturated Fatty Acids Influence Tissue Fatty Acid Composition in Rats at Weaning. J. Nutr. 126 :887-897. Suarez, A., M.J. Faus and A. Gil. 1996. Dietary supplementation with long-chain polyunsaturated fatty acids increases susceptiblity of weanling rat tissue lipids to in vitro lipid peroxidation. J. Nuh. Biochem. 7 :252-260. Sugano, M. and K. Imaizumi. 1995. Effect of different saturated fatty acids as interesterified triacylglycerols on lipid metabolism in rats and hamsters. J. Nutr. Biochem. 6 :195-200. Sugano, M., S. Goto, Y. Yamada, K. Yoshida, Y. Hashimoto, T. Matsuo and M. Kimoto. 1990. Cholesterol-Lowering Activity of Various Undigested Fractions of Soybean Protein in Rats. J. Nutr. 120 : 977-985. Sugiyama, K., A. Tanaka, H. Kawagishi, F. Ojima, H. Sakamoto and Y. Ishiguro. 1994. Hypocholesterolemic Action of Dietary GTifolin on Rats Fed with a High-cholesterol Diet. Biosci. Biotech. Biochem. 58 :211-212. Sugiyama, K., H. Kanamori, T. Akachi and A. Yamakawa. 1996. Amino acid composition of dietary proteins affects plasma cholesterol concentration through alteration of hepatic phospholipid metabolism in rats fed a cholesterol-hepatic phospholipid metabolism in rats fed a cholesterol-fiee diet. J. Nutr. Biochem. 7 :40-48. Sugiyama, K., T. Akachi and A. Yamakawa. 1995. Eritadenine-induced alteration of hepatic phospholipid metabolism in relation to its hypochotesterolemic action in rats. J. Nutr. Biochem. 6 :80-87.

Sulistyani. 1996. Terapi Estrogen dan Jantung Koroner. Harian KOMPAS Minggu, 14 Januari. Sundram, K., K.C. Hayes and O.H. Siru. 1995. Both dietary 18 : 2 and 16 : 0 may be required to improve the serum LDLIHDL cholesterol ratio in normocholesterolemic men. J. Nutr. Biochem. 6 :179-187.

.

Supari, F., A. Rasad dan A. Hanafiah. 1986. Pengaruh Diit Minyak Ikan Lemuru terhadap Kadar Lipid Plasma dan Agregasi Platelet pada Orang Sehat. Makalah. Universitas Indonesia. Jakarta. Sutantyo, E. 1994. The Effect of Palm Oil, Peanut Oil and Margarine on Serum Lipoproteins and Atherosclerosis in Rats. Gizi Indonesia - Journal of the Indonesian Nutrition Association I9 (I-2) : 69-89. Sutardi, T. 1997. Peluang dan Tantangan Pengembangan Ilmu-ilmu Nutrisi Temak. Orasi Ilmiah Guru Besar Tetap Ilmu Nutrisi Temak, Fakultas Petemakan IPB 4 Januari 1997; Swanson, Y.E., J.M.Black and J.E. Kinsella. 1987. Dietary n-3 Polyunsaturated Fatty Acids : Rate and Extent of Modification of Fatty Acyl Composition of Lipid Classes of Mouse Lung and Kidney. J. Nutr. 117 :824-832.

Takada, R., M. Saitoh and T. Mori. 1994. Dietary Y-Linolenic Acid-Enriched Oil Reduces Body Fat Content and Induces Liver Enzyme Activities Relating to Fatty Acid P-Oxidation in Rats. J. Nutr. 124 :469-474. Takahashi, K.and Y. Akiba. 1995. Effect of Methionine Supplementation on Lipogenesis and Lipolysis in Broiler Chicks. Jpn. Poult. Sci. 32 :99-106. Takita, T., K. Nakamura and S. Innami. 1994. Effects of Dietary Oils with Different n-3 Fatty Acid Sources and n-3/n-6 Ratios on Lipid Metabolism of Rats Fed on a High Cholesterol Diet. Biosci. Biotech. Biochem. 58 :695-698. Tavener, M. 1987. Evaluation of dry Kernzyrne brand enzyme as a dietary supplement for weaner pigs. Kemin Industries, Inc. publication no. 02246, pp. 1-7. Torreele, E., A.V.D. Sluiszen and J. Verreth. 1993. The effect of dietary L-camitine on the growth performance in fingerlings of the Afican catfish (Clarias gariepinus) in relation to dietary lipid. Br. J. Nutr. 69 :289-299. Vahouny, G.V., S. Satchithanandam, 1. Chen, S.A. Tepper. D. Kritchevsky. F.G. Lightfoot and M.M. Cassidy. 1988. Dietary fibre and intestinal adaptation : effects on lipid absorption and lymphatic transport in the rat. Am. J. Clin Nutr. 47 :201-206.

Van Der Klis, J.D. and A. Van Voorst. 1993. The Effect of Carboxy Methyl Cellulose (A Soluble Polysaccharide) on the Rate of Marker Excretion from the Gastrointestinal Tract of Broiler. Poult. Sci. 72 :503-512. Vanhanen, H. 1994. Cholesterol malabsorption caused by sitostanol ester feeding and neomycin in pravastatin-treated hypercholesterolaemic patients. European Journal of Clinical Pharmacologv 47 :169-1 76. Wainwright, P.E. H.-C. Xing, L. Mutsaers, D. McCutcheon and D. Kyle. 1997. Arachidonic Acid Offsets the Effects on Mouse Brain and Behavior of a Diet with a Low (n-6) : (n-3) Ratio and Very High Levels of Docosahexaenoic Acid. J. Nutr. 127 : 184-193. Wanasuria, S. 1990. Tepung Kepala Udang Indonesia No. 122iTh. X Hal. 19-21.

dalam Pakan Broiler.

Poultry

Wang, L., R.K. Newman, C.W. Newman and P.J. Hofer. 1992. Barley P-Glucans Alter Intestinal Viscosity and Reduce Plasma Cholesterol Concentrations in Chicks. J. Nutr. 122 :2292-2297. Watkis, B.A. 1991. Importance of Essential Fatty Acids and Their Derivatives in Poultry. J. Nutr. I21 : 1475-1 785. Watts, G.F., P. Jackson, V. Burke and B. Lewis. 1996. Dietary fatty acids and progression of coronary artery disease in men. Am. J. Clin. Nutr. 64 : 202-209 Wyan, C.L. and T.N. Goodman. 1993. Effect of Lcarnitine and dietary lysine and fat levels on growth performance and body lipid content in young broilers. Poult. Sci. 72 :200 (S 149). Xu, N. and A. Nilsson. 1996. Uptake and interconversion of plasma unesterified 14C linoleic acid by gastrointestinal tract and blood forming tissues : An experimental study in the rat. J. Nu&. ~io&hem.7 : 16-22. Yongrnanitchai, W . and O.P.Ward. 1989. Omega-3 Fatty Acids : Alternative Sources of Production. Process Biochemistry. 24 :11 7-125. Yu. S., J. Derr, T.D. Etherton and PM. Kris-Eltherton. 1995. Plasma cholesterolpredictive equations demonstrate that stearic acid is neutral and monounsaturated fatty acids are hypocholesterolemic. Am. J. Clin. Nutr. 61 : 1129-1139.

Lampiran 1. Prosedur Analisis Kolesterol Serum Darah Metode CHOD-PAP yang merupakan Tes Penuh dari Merckotest dengan rincian sebagai berikut : '

.

Kolorimetrik

Ensimatik

Reagensia : 1. Reagensia - warna A (3 x 80 ml) 2-(4-(2-HydroxymethyI)-perazinyl-ethensulfoacid)(PPES) 0.1 mol/l Phenol 12.0 mmoVl 2. Reagensia - warna B (3 x 2 ml) 0.08 mol/l 1-phenyl-2,3-dimethyl-4-aminopyrazolone (5) 3. Campuran ensim (I x 3.3 ml) > 10 k U/1 cholesterol esterase; > k UA cholesterol oxidase; > 85 k U/1 peroxidase (menurut Gallati) Semua reagensia akan tetap stabil hingga tanggal kedaluwarsa bila masih tertutup rapat dan disimpan pa& suhu +2 hingga +S°C. Persiapan : Larutan pereaksi Tambahkan 1 ml dari botol 3 (campuran ensim) dan isi satu botol ke dalam botol 1 (reagensia - warna A) clan campurkanlah dengan seksama. Campuran ini stabil selama 4 minggu bila disimpan pada suhu +2 hingga +8'C dan selama 1 minggu pada +15 hingga +25OC. Simpanlah di tempat yang terhindar dari cahaya. Prosedur : Panjang gelombang : 500 nm,546 nm,Filter 546 nm. Diameter dalam kuvet : 1 cm. S e r b atau plasma 10 pl Larutan pereaksi 1000 p1 Campurkanlah dan inkubasikan selama 10 menit pada suhu + 15 hingga +25'C atau selama 5 menit pada suhu +37'C. Ukur absorbance dari sampel (A) terhadap larutan pereaksi. Absorbance akan tetap stabil selama 45 menil.

Perhitungan : Konsentrasi kolesterol = A.F

Batas Pengenceran :

Apabila konsentrasinya lebih tinggi, encerkan sampel 1 + 2 dengan gararn isotonik (9 g/l A 154 mmoV1 NaCl), ulangi penentuan dan kalikan hasilnya dengan 3.

Lampiran 2. Analisis Lipid dalam Plasma atau Serum

Analisis lemak atau lipid dalam serum darah meAurut Dawiesah (1989) dalam buku Petunjuk Laboratorium Penentuan Nutrient dalam Jaringan clan Plasma Tubuh, cara kerjanya adalah sebagai berikut : Bahan : 1. Alkohol bebas lemak. Diperoleh dengan menyuling dua kali etanol96%. 2. Petroleumeter bebas lemak (titik didih 40-60' C), diperoleh dengan menyuling dua kali petroleumeter. Pelaksanaan : Seluruh penentuan dilakukan dengan alat gelas bebas lemak dan dengan pipet lemak. Dipipet 5 ml serum dalam labu ukur 100 ml yang mengandung kurang lebih 70 ml alkohol. Sesudah digojok baik-baik didiarnkan 10 menit, diisi sampai marka dengan alkohol, dicampur clan disaring. Dari hasil saringan dipipet 80 rnl, sesuai dengan 4 ml serum, dalam cawan porselin dan dikeringkan hati-hati pada penangas air mendidii. Sisa yang tertinggal dilarutkan dengan porsi-porsi kecil petroleum eter, yang setiap kali sebentar ditempatkan pada penangas air sampai mendidih. Larutan disaring dalam botol timbang kecil yang sudah ditimbang. Sesudah corong dibilas dengan petroleumeter, cairan dalam botol timbang diuapkan pada penangas air. Seterusnya botol timbang ditempatkan dalam eksikator untuk mendiginkan dan ditimbang. Perbedaan penimbangan sebelum dan sesudah penentuan memberikan jumlah lipid. Dengan dikalikan 25, kadar lemak diperoleh dalam mg%.

Lampiran 3. Penentuan Kadar Kolesterol dengan Metode Liebermann-Burchard Sampel daging ditimbang sebanyak 1 gram diiasukkan ke dalam tabung reaksi, kemudian diekstraksi dengan aseton alkohol dengan perbandingan 1 : 1 sebanyak 10 ml, diaduk sampai daging hancur. Tabung tersebut dipanaskan dalam penangas air sampai mulai mendidih. Tabung diangkat dan digoyang selama 5 menit, kemudian didinginkan. Setelah diigin filtratnya dimasukkan tabung sentrifuge dan disentrifuge selama 15 menit dengan kecepatan 2500 rpm. Supematan yang terbentuk dimasukkan tabung reaksi dan diuapkan dengan dipanaskan &lam air mendidii sampai kering dan terbentuk residu. Residu kering ini dilarutkan dengan 3 ml khloroform dan diiomogenkan. Kemudian ditambah larutan asetat anhidrid dan asam sulfat pekat dengan perbandingan 30: 1, ditempatkan dalam ruang gelap sekitar 5 menit sehingga larutan berwarna hijau kebirum. Dengan cara yang sama dibuat larutan blanko. Hasil analisis ditera dengan spektrofotometer pada panjang gelombang 680 nrn. Perhitungan kadar kolesterol daging dilakukan dengan membandingkan absorbannya dengan absorban kolesterol standar (Plummer, 1977). Cara pembuatan larutan kolesterol murni untuk larutan standar : Pertama-tama membuat larutan kolesterol murni dengan konsentrasi 2 mg/ml, sebanyak 10 ml dan diencerkan dengan 40 ml khloroform, sehingga terbentuk' larutan dengan konsentrasi 0.4 mgfml (larutan A). Kemudian dibuat larutan kolesterol murni dengan bermacam-macam konsentrasi dari pengenceran larutan A. Tahap selanjutnya adalah larutan A diambil20 ml diiasukkan tabung reaksi dan ditambah khloroform 10 ml sehingga diperoleh larutan dengan konsentrasi 0.2 mglml (larutan B). Dari larutan B diambil masing-masing 9 ml, 7 ml, 6 ml, 5 ml, 4 ml, 3 ml dan 2 ml. Dari pengenceran larutan B ini akan diperoleh larutan dengan konsentrasi0.18, 0.14,0.12, 0.10,0.08, 0.06,0.04mg/ml. Larutan A diambil lagi sebanyak 30 ml (larutan C). Dari larutan C ini diambil 8 ml, 7 ml, 6 ml, 5 ml dan 4 ml. Masing-masing dimasukkan ke dalam tabung reaksi kemudian volumenya disamakan menjadi 10 ml, sehingga diperoleh larutan dengan konsentrasi 0.32,0.28, 0.24,0.20 dan 0.16 mg/ml. Larutan standar tersebut masing-masingdiarnbil 3 ml dan ditambahkan 3 n ~ asetat l anhidrid - asam sulfat pekat dengan perbandingan 30:1, ditempatkan pada ruang gelap selama 5 menit hingga larutan menjadi hijau, diukur absorbansinya dengan spektrofotometer pada panjang gelombang 680 nm (Plummer, 1977).

Absorban larutan kolesterol standar : -

No.

I. 2. 3.

4. 5. 6. 7. 8. 9. 10. 11. 12.

-

-

-

--

Kadar kolesterol standar (mglml)

0.04 0.06 0.08 0.10 0.12 0.14 0.16 0.18 0.20 0.24 0.28 0.32

Persamaan garis regresi (r)

Y = 2.355436 x + 0.00565 r = 0.99816 x = Kadar kolesterol Y = Absorban

Absorban 0.J 135 0.1322 0.1972 0.2328 0.3098 0.3188 0.3768 0.4173 0.4914 0.5808 0.6625 0.7570

.

Lampiran 4. Prosedur Analisis LDL-Cholesterol

.

Metode CHOD-PAP yang merupakan Tes Kolorimetrik Ensimatik Penuh yang urutannya adalah sebagai berikut : Reagensia : I. Reagensia - pengendap, 250 ml (0.68 g/l heparin, kira-kira 100.000 IU/I; 0.064 molll sodiumsitrat, stabilizer). 2. Larutan pereaksi untuk penentuan kolesterol Prosedur : Absorbance rnaksimal : 500 nm,546. Filter : 546 run. Diameter dalam kuvet : 1 cm. Hangatkan reagensia pengendap hingga suhu antara +15 hingga +25OC Pipetkan ke dalam tabung sentrifugasi : Serum : 100 pl Reagensia - pengendap 1 : 1000 pl Campwkanlah dengan seksama, biarkan selama 10 menit pada suhu +15 hingga +25OC dan lakukan sentrifugasi selama 15 menit pa& kurang lebii 4000 rpm. Tentukan konsentrasi kolesterol &lam supematan, dalam waktu 1 jam setelah sentrifugasi. Pipetkanlah ke dalam bejana reaksi : Supematan : 100d Larutan pereaksi 2 : 1000 p1 Campurkanlah dengan seksama, inkubasikan selama 10 menit pada suhu +15 hiigga +25OC atau selama 5 menit pa& suhu +37"C dan ukurlah absorbance sarnpel (A) terhadap larutan pereaksi. Perhitungan : Konsentrasi kolesterol dalam supernatan : A.F

Perhitungan LDL-cholesterol : LDL cholesterol = total kolesterol - kolesterol dalam supematan

Lampiran 5. Prosedur Analisis HDL-Cholesterol Metode CHOD-PAP yang merupakan Tes Penuh yang urutannya adalah sebagai berikut :

Kolorimetrik

Ensimatik

Reagensia : 1. Reagensia pengendap, 250 ml (1.4 mmol phosphotungstic acid; 8.6 mmolll magnesium chloride; stabilizer). 2. Larutan pereaksi untuk penentuan kolesterol

-

Prosedur : Absorbance maksimal : 500 tun. Filter : 546 nrn. Diameter dalam kuvet : 1 cm. Pipetkan ke dalam tabung sentrifugasi : : 200 pl Senun Reagensia pengendap 1 : 500 pl

-

Campurkanlah dengan seksama, biarkan selama 10 menit pada suhu +15 hiigga +2S°C clan kemudian lakukm sentrifugasi selama 15 menit pada 4000 rpm. Dalam waktu dua jam setelah sentrifugasi, gunakan supematan yang jernih (lihat catatan) untuk penentuan konsentrasi kolesterol dengan metode CHOD-PAP. Sampel Pipetkan ke dalam tabung reaksi : Supematan Aquabides Larutan pereaksi

100 pl

-

1000 p1

Blanko reagensia

100 pi 1000 p1

Campurkanlah dengan seksama, inkubasikan selama 10 menit pada suhu +15 hingga +25OC atau selama 5 menit pa& suhu +37OC dan ukurlah absorbance sampel (A) terhadap blanko reagensia. Perhitungan : Konsentrasi HDLcholesterol = A.F

Catatan :

Supematan yang terbentuk sesudah sentrifugasi harm jemih. Serum yang mengandung trigliserida lebih dari 1000 mgtdl cenderung untuk memproduksi supematan yang keruh atau endapan yang mengapung. Apabila ha1 ini terjadi, encerkan sampel 1+1 dengan garam isotonik (9 g/l A 154 mmolR\laCl) dan kemudian lakukan lagi pengendapan. Kalikan hasilnya dengan 2.

Lampinn 6. Prosedur Analisis Trigliserida

Metode GPO-PAP Tes Kolorimetrik Ensimatik Penuh yang mtannya sebagai berikut : Reagensia : 1. Larutan buffer (80 ml) 2. Carnpuran reagensia (untuk 7 x 1l ml) 3. Larutan standar (1 x I ml)

Semua reagensia akan tetap stabil hingga tanggal kedaluwarsa bila masih tertutup rapat dan disimpan pa& +2 hiigga +g°C. Persiapan : 1. Larutan pereaksi (0.1 mill PIPES pH 7.5; 12.8 mmolll magnesium acetate; 1.0 mrnol/l 4-aminoantipyrine; 2 mmol1lATP; 2.0 mmoM 2-chlorophenol; 1 U/ml peroxidase; 0.2 Ulml glycerolkinase; 1.5 Ufml glycerophosphate oxidase; 10 Ulml lipase; 2 gfl polyethyleneglycol monomethylether; stabilisator; aktivator). Larutkan isi satu botol2 ke dalam 11 rnl larutan buffer dari botol 1. 2. Larutan standar (200 mg/dl A 2.29 mmol). Larutan standar dalam keadaan siap pakai. Larutan ini akan tetap stabil hingga tanggal kedaluwarsa bila masih t e m p r a p t dan disimpan p d a suhu +2 hingga +8OC. Prosedur :

Panjang gelombang : 492 nm,500 nm, 546 nm. Filter :Hg 546, Hg 492. Diameter &lam kuvet : 1 cm. Bangatkan larutan pereaksi pada suhu +20 hingga +2S°C. Hasil dapat dihitung dengan menggunakan sebuah standar atau faktor. Sampel Senun atau plasma Larutan standar (2) Larutan pereaksi (1 )

Standar

10 d

-

100 pl

10 pl 1000 p1

Campurkanlah dan inkubasikan selama 10 menit pada suhu +15 hingga +25"C. Ukurlah absorbance sampe1 (As) dan absorbance standar (Ast) terhadap larutan pereaksi &lam waktu 1 jam.

Perhitungan : Penentuan dengan standar : 200 Konsentrasi trigliserida = As . ----- (mgldl) Ast 2.29 = As

. ------

(mmol/l)

Ast Penentuan dengan faktor : Konsentrasi trigliserida = As .F

Batas pengenceran : 1000 mgldl A 11.4 mmol/l Bila konsentrasi trigliserida lebih tinggi, encerkan sampel 1 + 5 dengan larutan garam isotonik (9 g/l A 154 mmolfl NaCl), ulangi penentuan dan k a l i i hasilnya dengan 6.

Lampiran 7. Prosedur Analisis Asam Lemak dengan Kromatografi Gas

,

Analisis asam lemak pada daging, pakan dan minyak ikan dilakukan dengan alat kromatografi gas, dengan rincian sebagai berikut : Tahap I :Ekstraksi Minyak (Bligh dan Dyer) Sampel berupa daging ayam, hams bersih dari kulit dan tulang. Lumatkan daging yang sudah diblender dengan cepat menggunakan mortir. Ambil25 g lurnatan daging, masukkan dalam erlenmeyer 250 ml. Tambahkan 25 ml khloroform dan 50 ml metanol dan gojog selama 5 menit. Saring cairannya (filtrat I) dari padatannya dengan 25 ml khlorofonn sambil digojog selama 5 menit. Pisahkan lagi cairannya (filtrat 11) dan campurkan dengan filtrat I. Saring melalui saringan gelas atau sentrifuge untuk memisahkan kotoran. Masukkan cairan ekstrak ke dalam gelas silinder 250 ml. Tambahkan 25 ml larutan KC1 0.88% dalam air ke dalam ekstrak, gojog dan biarkan mendendap. Larutan yang di bawah berisi lipid hasil ektraksi dipisahkan dan diuapkan pelarutnya dengan menggunakan aliran gas Nz Sampel diambil dengan sediit heksana (diietahui jumlahnya) dan dilanjutkan dengan proses berikutnya atau disimpan di almari es.

Tahap I1 : Ekstraksi atau metilasi

Buat larutan 5% minyak dalam heksana, ambil6 ml larutan ini dan masukkan ke dalam tabung reaksi yang tertutup (kapasitas 12 mi). Tambahkan 150 $ 2 N KOH dalam metanol clan gojog selama 5 menit. Sentrifugasi selama 5 menit pada 2000 rpm, supernatannya yang diperoleh siap untuk diinjeksikan pada alat kromatografi gas. Tahap 111 :Penentuan ester asam lemak dengan kromatografi gas Ester-ester asam lemak yang telah disiapkan diinjeksikan meialui injektor alat kromatografi gas untuk pemisahan dan identifikasi serta penentuan kuantitatif asam-asam lemak dapat dilakukan dari kromatogram yang diperoleh. Kromatografi gas yang digunakan merk HITACH1 163 GC, dilengkapi dengan Flame ionization Detector (FID)dan integrator merk SHIMADZU C-R 6A Chromopac.

Kondisi kromatografi gas yang digunakan : Kolom Bahan isian

: gelas, panjang 2 m, diameter 3 mm

Gas pembawa Gas pembakar Gas pembantu Suhu kolom

: Nzkecepatan 50 mllmenit : H2 kecepatan 90 mllmenit

Suhu injektor Kecepatan kertas Volume injeksi

: 255OC : 2 rndmenit

: 10% DEGS (dietil glikol suksinat)

dan Chromo 8Ol100 : udara, tekanan 1.9 kg/cm2

: suhu awal 195' C

suhu akhir 205'C

: 1-2 mikroliter

.

Lampiran 8. Uji Pengikatan Serat terhadap Lemak OBS UL SELULOSA CHITIN AGAR SEL+CHTN SEL+AGAReCHT+AGAR 1 1 53.13 78.13 0.00 84.38 . 6.25 75.00 2 2 53.13 71.88 3.13 65.63 3.13 59.38 3 3 56.25 78.13 3.13 43.75 6.25 53.13 Analysis of Variance Procedure Class Level Information Class

Levels

Values

PERL

6

U1.

3

Agar Chitin Chitin+Agar Selulosa Selulosa+Agar Selulosa+Chitin 1 2 3

Number of observations in data set = 18 General Linier Models Procedure Dependent Variable: IKAT-LMK Sum of Squares

Mean Square

3094.75973 7943.460939 717.882817 0.000006 93.86372

32.97 84.63 7.65 0.00

12

15473.79863 7943.460939 717.882817 0.000006 1126.36467

17

16600.16329

Root MSE 9.688329

IKAT-LMK Mean 44.1005556

~ r / >T I

Std Error of Estimate 13.7013665 7.9104876 13.7013665

Source

DF

PERL 1-n v s III

5 1 1

I vs U I UI vs IV- V] &or

Corrected Total

1

R-Square 0.932147 Parameter 1-11 vs III 1 vs I1 1-111 vs 1V-VI

C.V. 21.96872

TforHO= Parameted Estimate -126.0433333 -9.20 2 1.87666667 2.77 -0.00333333 - 0.00

'

0.0001 0.0171 0.9998

FValue

Pr > F

0.0001 0.0001 0.0171 0.9998

Duncan's Multiple Range Test for variabel: IKAT-LMK NOTE : This test controls the type I comparisonwise error rate, not the experimentwise error rate

MSE = 93.86372

Alpha= 0.05 df = 12 Number of Means Critical Range

5 6 3 4 2 17.20 18.02 18.57 18.85 19.06

Means with the same letter arenot significantly differentt. Duncan Grouping

Mean

N

PERL

76.047 64.587 62.503 54.170 5.210 2.087

3

Chitin Selulosa+Chitin Chitin+Agar Selulosa Selulosa+Agar Agar

3 3 3 3 3

Lampiran 9. Contoh : ANOVA Pertambahan Berat Badan

.

General Linier Models Procedure Class Level Information Class

Levels

Values

KELAMIN PERL

2

Betina Jantan I II m IV v VI

7 3

UL

1

2

vn

3

Number of observations in data set = 42 General Limier Models Procedure Dependent Variable : BRT-BDN Source

Squares

Mean Square

KELAMIN

387840.3810 920582.4762 297601.5873 429664.5000 196982.7222 194820.0833 18723.3611 140060.9524

387840.38 10 153430.4127 297601.5873 429664.5000 196982.7222 194820.0833 18723.3611 23343.4921

486 142.667 1934626.476

17362.238

C.V.

Root MSE

TforHO=

~ r >

ITI

Std Error of Estimate

0.0003 0.000 1 0.0022 0.0023 0.3079

348.619659 186.345046 186.345046 76.075046 131.765846

Sum of

PEG I vs n-VII I vs II-N IV vs v-VII Ivvsw v-vl vs VII KEL.Ah,nN+PERL Error Corrected Total

28 41

R-Square

Parameter 1 vs 11- VH I vs 11-N IV vs V-VII IV vs VI1 V-VI vs V11

Estimate 1443.3333333 927.00000000 -627.6666667 -254.8333333 -136.8333333

Parameted 4.14 4.97 - 3.37 - 3.35 - 1.04

F Value 22.34 8.84 17.14 24.75 11.35 11.22 1.08 1.34

BRT-BDN Mean

Duncan's Multiple Range Test for variabel: BRT-BDN NOTE : This test controls the type I comparisonwise error rate, not the experimentwise error rate

.

Alpha= 0.05 df = 28 Number of Means Critical Range

MSE = 17362.24

2 83.24

Means with the same letter are not significantly differentt. Duncan Grouping

Mean

-

2243.29 2051.10

N

PERL

21 21

Jantan Betina

Duncan's Multiple Range Test for variabel: BRT-BDN NOTE : This test controls the type I comparisonwise error rate, not the experimentwise error rate Alpha= 0.05 Number of Means Critical Range

df = 28

MSE = 17362.24

2 3 4 5 6 7 155.7 163.7 169.1 172.5 175.3 177.5

Means with the same letter are not significantly differentt. Duncan Grouping A A A A A A B B B B B B B

Mean 2322.50

PERL

m

Lampiran 10. Data Pertambahan Berat Badan (g) lenis kelamin Ulangan Perlakuan 1 Perl. 11 Perl. I11 Perl. IV Pert. V Perl. VI Perl. VII

Rataan

Rataan

Perlakuan

I

I1

III IV

v

VJ VII

= Ransum basal = Ransum basal = Ransum basal

+ 100% Selulosa

+ 100%

Khitin

= Ransum basal + 100% Agar = Ransum basal + 50% Selulosa + 50% Khitin = Ransum basal + 50% Selulosa + 50% Agar = Ransum basal + 50% Khitin + 50% Agar

Lampiran 11. Data Konsumsi Pakan (g) Jenis kelamin Ulangan Perlakuan l Perl. 11 Perl. 111 Perl. IV Perl. V Perl. V1 Pui. VI1

Rataan Betina

1

2 3 4 5

Rataan

Keterangan : Perlakuan

I I1 Ill

IV V V1 VII

= Ransum basal

+ 100% Selulosa = Ransum basal + 100% Khitin = Ransum basal + 100% Agar

= Ransum basal

= Ransum basal = Ransum basal

+ 50% Selulosa + 50% Khitin

= Ransum basal

+ 50% Khitin + 50% Agar

+ 50% Selulosa + 50%Agar

Lampiran 12.

.

Data Konversi Pakan

Jenis kelamin Ulangan Perlakuan 1 Perl.I1 Perl.111 Perl.IV Perl. V Perl.V1 Perl.VII

Rataan

Rataan

Keterangan :

Perlakuan

I II

= Ransum basal

ID IV

= Ransum basal + 100% Khitin = Ransum basal + 100% Agar

V VI VII

+ 50% Selulosa + 50% Khitin = Ransum basal + 50% Selulosa + 50% Agar = Ransurn basal + 50% Khitin + 50% Agar

= Ransum basal +

= Ransum basal

100% Selulosa

Lampiran 13. Data Kolesterol Ekskreta (mg %) Jenis kelamin Ulangan Perlakuan l Perl. I1 Perl. I11 Perl. lV Perl. V Perl. VI --

Jantan

1 2 3 4 5

Rataan Betina

1

2 3 4

5

Rataan

Keterangan :

Perlakuan

I

II IU IV V VI VII

= Ransum = Ransum

basal basal + 100% Selulosa = Ransum basal + 100%Khitin = Ransum basal + 100%Agar = Ransum basal + 50% Selulosa + 50% Khitin = Ransum basal + 50% Selulosa + 50% Agar = Ransum basal + 50% Khitin + 50% Agar

Perl. VII

Lampiran 14.

.

Data Lemak Ekskreta (%)

Jenis kelamin Ulangan Perlakuan l Perl. 11 Perl. Ill Perl. IV. Perl. V Perl. VI Perl. VII

Rataan Betina

1 2 3 4 5 Rataan

2.46

5.22

6.50

2.67

5.35

3.70

3.70

2.68 2.32 2.41 2.56 2.21

5.63 5.66 4.40 4.72 5.47

6.72 7.86 5.89 5.72 7.14

2.76 2.42 2.38 2.48 2.51

5.54 5.02 5.07 5.31 5.21

2.83 3.46 3.62 3.51 3.49

4.08 3.52 3.38 3.42 3.61

2.44

5.18

6.67

2.51

5.23

3.38

3.60

Keterangan :

Perlakuan

I 11 III

IV V

VI VII

= Ransum basal = Ransum basal + 100% Selulosa = Ransum basal + 100% Khitin = Ransum basal + 100% Agax

basal + 50% Selulosa + 50% Khitin + 50% Selulosa + 50% Agar = Ransum basal + 50% Khitin + 50% Agar = Ransum

= Ransum basal

.

Data Serat Kasar Ekskreta ( O h )

Ulangan Pertakuan 1 Perl. 11 Perl. 111 Perl. lV Perl. V Perl. VI Pert. V11

Jenis kelamin

Rataan

13.94

14.22

16.64

14.13

16.09

14.41

15.00

1 2 3 4 5

12.47 11.60 12.86 11.92 11.35

14.73 15.05 14.92 15.01 14.74

17.14 16.40 16.72 17.01 16.58

12.96 12.66 12.72 13.02 12.69

14.98 17.11 16.02 17.09 15.05

14.90 13.88 14.46 13.98 14.73

14.69 15.00 14.87 14.68 15.01

12.04

14.89

16.77

12.81

16.05

14.39

14.85

Betina

Rataan

Keterangan : Perlakuan

I II

III IV V VI VII

= Ransum basal = Ransum basal + 100% Selulosa = Ransum basal + 100% Khitin = Ransum basal + 100% Agar = Ransum basal + 50%

Selulosa + 50% Khitin = Ransum basal + 50% Selulosa + 50% Agar = Ransum basal + 50% Khitin + 50% Agar

Lampiran 16. Data Kolesterol Daging (mg %) Ulangan Perlakuan 1 Perl. 11 Perl. 111 Perl. I\!

Jenis kelamin

Perl. V Perl. VI Perl. VI1

- ---

Jantan

76.13 77.09 80.1 2 80.72 79.82

1

2

3 4 5

Rataan

70.96 73.72 74.89 72.07 73.80

63.83 50.56 59.36 63.80 64.09

62.62 80.14 77.04 68.79 79.98

67.02 68.71 66.21 65.80 68.09

68.03 74.01 73.82 72.90 71.97

67.46 69.72 68.07 68.71 67.95

78.78

73.09

60.33

73.71

67.17

72.15

68.38

4 5

77.42 79.82 80.06 81.01 76.07

72.07 76.03 72.89 70.02 71.87

64.67 58.70 62.86 61.98 60.71

74.89 76.82 80.12 72.69 70.81

70.13 65.82 66.80 68.92 68.01

73.72 71.06 72.89 70.91 71.68

80.72 62.70 66.02 65.21 63.38

Rataan

78.88

72.58

61.78

75.07

67.94

72.05

67.61

Betina

1 2

3

Keterangan :

Perlakuan

I

= Ransum basal

II EI IV V VI

= Ransum basal + 100% Selulosa = Ransum basal + 100% Khitin = Ransum basal + 100% Agar = Ransum basal + 50% Selulosa + 50% Khitin = Ransum basal + 50% Selulosa + 50% Agar = Ransum basal + 50% Khitin + 50% Agar

VII

Lampiran 17. Data Lemak Daging (%) Jenis kelamin Ulangan Perlakuan I Perl. 11 Perl. Ill Perl. lV Perl. V PerlrVl Perl. V11 Jantan

1 2

3

4 5

Betina

..

1 2 3 4 5

Keterangan : Perlakuan

I I1

= Ransum basal +

= Ransum basal

III

= Ransum basal

IV V VI VII

= Ransum basal

100% Selulosa

+ 100% Khitin

+ 100% Agar = Ransum basal + 50% Selulosa + 50% Khitin = Ransum basal + 50% Selulosa + 50% Agar = Ransum basal

+ 50% Khitin + 50% Agar

Lampiran 18. Data Protein Daging (%) Jenis kelamin Ulangan Perlakuan I Perl. I1

Perl. 111 Perl. IV Perl. V Perl. VI Pnl. VII

Rataan

18.16

20.53

22.64

20.31

21.41

20.38

21.07

1 2 3 4 5

21.94 17.43 18.98 19.72 20.3 8

22.77 18.96 20.56 21.07 20.99

26.13 20.70 24.02 23.88 22.37

20.46 20.60 21.02 20.51 20.06

21.25 21.96 21.66 21.42 21.61

20.55 21.12 20.70 20.62 21.21

20.89 21.20 21.01 20.92 21.23

Rataan

19.69

20.87

23.42

20.53

21.56

20.84

21.05

Betina

Keterangan : Perlakuan

I

D Dl N V VI

VII

= Ransum basal = Ransum basal + 100% Selulosa = Ransum basal + 100% Khitin

+ 100% Agar basal + 50% Selulosa + 50% Khitin = Ransum basal + 50% Selulosa + 50% Agar = Ransum basal + 50% Khitin + 50% Agar = Ransum basal = Ransum

Lampiran 19.

.

Data Air Daging (%)

Jenis kelamin Ulangan Perlakuan 1 Perl. 11 Perl. 111 Perl. IV Perl. V Perl. V1 Perl. VII

Betina

1 2 3 4 5

74.44 74.92 74.51 75.68 75.03

75.62 74.40 74.75 74.86 74.66

74.82 74.36 75.23 74.91 75.21

75.66 75.67 75.16 74.15 75.17

75.69 74.66 74.85 74.18 74.85

75.06 75.50 72.29 74.61 75.59

Keterangan : Perlakuan

I 11

III N V VI VJI

= Ransum basal = Ransum basal +

100% Selulosa = Ransurn basal + 100% Khitin = Ransum basal + 100% Agar = Ransum basal + 50% Selulosa + 50% Khitin = Ransum basal + 50% Selulosa + 50% Agar = Ransum basal + 50% Khitin + 50% Agar

76.02 75.47 76.11 74.79 74.95

Lampiran 20. Data Kolesterol Darah (mg/dl)

Jenis kelamin Ulangan Periakuan 1 Perl. I1

Pal. UI Perl. IV Perl. V Perl. V1 Perl. VII

Jantan

1 2 3 4 5

Rataan 1 2 3 4 5

Betina

Rataan

122.92 124.10 121.96 117.80 121.52

109.08 106.78 104.02 98.76 106.71

98.07 101.01 99.08 102.45 97.09

120.92 122.14 122.68 121.96 120.60

101.08 98.07 101.68 99.01 97.86

112.71 109.68 104.97 118.02 107.62

101.68 102.97 99.81 105.37 101.72

121.66

105.07

99.54

121.66

99.54

110.60

102.31

95.06 96.07 91.83 89.08 98.01

84.72 86.76 81.71 80.58 90.98

71.02 68.71 70.09 66.12 69.71

94.71 93.68 84.38 90.86 92.62

76.81 72.86 70.92 69.08 69.78

88.01 86.07 78.09 81.57 81.01

76.01 77.82 73.62 73.21 72.64

94.01

82.95

69.13

91.25

71.89

82.95

74.66

Keterangan : Perlakuan

I 11

= Ransum basal

III N

= Ransum basal + = Ransum basal +

V VI VII

= Ransum basal

+ 100% Selulosa

100% Khitin 100%Agar = Ransum basal + 50% Selulosa + 50% Khitin = Ransum basal + 50% Selulosa + 50% Agar = Ransum basal + 50% Khitin + 50% Agar

Lampiran 21. Data Lemak Serum Darah (mg %)

Jenis kelamin Ulangan Perlakuan l Perl. I1 Perl. Ill Perl. lV Perl. V Perl. VI Perl. VI1

Rataan Betina

1

2 3 4 5

Rataan

Keterangan :

Perlakuan

I

= Ransum basal

IJ

= Ransum

ID N V VI VII

basal + 100% Selulosa = Ransum basal + 100% Khitin = Ransum basal + 100% Agar = Ransum basal + 50% Selulosa + 50% Khitin = Ransum basal + 50% Selulosa + 50% Agar = Ransum basal + 50% Khitin + 50% Agar

Lampiran 22.

.

Data Lemak Terrnetabolis (%)

Jenis kelamin Ulangan Perlakuan 1 Perl. 11 Perl. 111 Perl. lV Perl. V Perl. VI Perl. V11

Rataan

88.79

85.72

74.21

86.21

80.01

82.92

81.21

Rataan

89.20

84.61

75.68

87.92

79.61

83.72

82.46

Keterangan : Perlakuan

I I1

III N V VI VII

= Ransum basal

basal + 100% Selulosa = Ransum basal + 100%Khitin = Ransum basal + 100%Agar = Ransum basal + 50% Selulosa + 50% Khitin = Ransum basal + 50% Selulosa + 50% Agar = Ransum basal + 50% Khitin + 50% Agar = Ransum

Lampiran 23. Data Pertambahan Berat Badan (g)

Jenis kelamin Ulangan Perlakuan 1 Perl.I1 Perl.111 Perl.IV Perl.V Perl.VI Perl.VII

Jantan

1

2 3 Rataan

Rataan

Perlakuan

I

II

= Ransum basal = Ransum basal + Kemzim

IV

= Ransum basal

VI W

= Ransum basal + Mikroelemen = Ransum basal + Lisin + Metionin + Mikroelemen

m

v Mikroelernen :

+ Sodium pravastatin + Karnitin = Ransum basal + Lisin + Metionin = Ransum basal

- Asam askorbat - Piridoksin - Niacin -

FeSQ

Lampiran 24. Data Konsumsi Pakan (g)

Jenis kelamin Ulangan Perlakum l Perl.I1 Perl.111 Perl.IV Perl.V Perl.VI Perl.VII

Rataan

5848

5681

5642

4968

6303

5208

5504

Rataan

4876

4648

5714

4509

5092

5232

4522

Perlakuan

I

ll III

IV V VI VII

Mikroelemen :

= Ransum basal = Ransum basal + Kemzim = Ransum basal + Sodium pravastatin = Ransum basal + Karnitin = Ransum basal + Lisin + Metionin = Ransum basal + Mikroelemen = Ransum basal Lisin + Metionin

+

-

-

Asam askorbat Piridoksin Niacin FeS04

+ Mikroelemen

Lampiran 25. Data Konversi Pakan

Jenis kelamin Ulangan Perlakuan I Perl. I1 Perl. I11 Perl. IV Perl. V Perl. VI Perl. VII

Jantan

1 2 3

2.93 2.79 2.87

2.52 2.57 2.63

2.39 2.61 2.20

1.92 2.20 2.04

2.84 2.65 2.45

2.57 2.87 2.36

2.46 2.37 2.41

Betina

1 2 3

2.64 2.46 2.83

2.36 2.32 2.29

2.34 2.66 2.49

2.03 2.13 1.94

2.40 2.26 2.33

2.59 2.66 2.76

2.44 2.56 2.35

I II

Ill N

= Ransum basal = Ransum basal + Kemzim = Ransum basal + Sodium pravastatin = Ransum basal + Karnitin

V VI VII

basal + Lisin + Metionin = Ransum basal + Mikroelemen = Ransum basal + Lisin + Metionin + Miluoelemen

Keterangan : Perlakuan

Mikroelemen :

= Ransum

-

-

-

Asam askorbat Piridoksin Niacin FeSOn

Lampiran 26. Data Kolesterol Daging (mg O h ) Jenis kelamin Ulangan Perlakuan I Perl. I1 Perl. I11 Perl. IV Perl. V Perl. VI Perl. V11

Rataan 69.15

65.42

61.27

52.27

60.79

61.81

54.26

Rataan 60.60

49.86

47.89

43.80

50.40

47.90

43.31

Keterangan : Perlakuan

I I1

In

= Ransum basal + Sodium pravastatin

+ Karnitin

IV

= Ransum basal

VI

= Ransum basal + Lisin + Metionin = Ransum basal + Mikroelemen

VII

= Ransum basal

v Mikroelemen :

= Ransum basal = Ransum basal + Kemzirn

-

+ Lisin + Metionin + Mikroelemen

Asarn askorbat Piridoksin Niacin FeS04

Lampiran 27. Data Lemak Daging (%)

Jenis kelamin Ulangan Perlakuan 1 Perl. 11 Pal. 111 Perl. IV Pal. V Perl. VI Perl. VII

Rataan 3.50

2.76

2.85

1.98

2.74

2.47

2.81

3.99 3.18 3.81

3.29 3.15 3.23

2.81 2.41 3.28

2.11 2.02 2.32

2.92 2.84 2.53

2.63 2.41 3.51

2.95 3.09 2.81

Rataan 3.66

3.22

2.83

2.15

2.76

2.85

2.95

Betina

1

2 3

Keterangan : Perlakuan

I I1

rn N

v

VI VII Mikroelemen :

= Ransum basal = Ransum basal + Kemzim = Ransum basal + Sodium pravastatin = Ransum basal + Karnitin

basal + Lisin + Metionin basal + Mikroelemen = Ransum basal + Lisin + Metionin + Mikroelemen = Ransum = Ransum

-

Asam askorbat Piridoksin Niacin FeS04

Lampiran 28. Data Air Daging (%)

Jenis kelamin Ulangan Perlakuan 1 Perl. 11 Perl. I11 Perl. IV Perl. V Perl. VI Perl. V11

Jantan

1 2 3

72.59 76.45 74.62

72.12 75.25 73.79

73.98 75.05 74.62

73.29 74.90 74.20

73.54 75.19 74.47

73.69 73.61 73.75

74.18 73.69 73.84

Rataan 74.55

73.72

74.55

74.13

74.40

73.68

73.90

Rataan 74.87

74.86

73.33

74.95

75.93

74.52

74.24

Keterangan : = Ransum basal = Ransum basal + Kemzim

+ Sodium pravastatin = Ransum basal + Kamitin = Ransum basal + Lisin + Metionin = Ransum basal + Mikroelemen = Ransum basal + Lisin + Metionin + Mikroelemen = Ransum basal

Mikroelemen :

-

Asam askorbat - Piridoksin - Niacin - FeS04

Lampiran 29.

.

Data Protein Daging (%)

Jenis kelamin Ulangan Perlakuan l Perl. I1 Perl. Ill Perl. IV Perl. V Perl. V1 Perl. V11

Rataan 18.34

Betina

1 2 3

19.17

21.70

20.92

20.93

20.36

23.24

20.91 17.80 21.19

21.11 22.00 21.39

23.19 24.04 21.54

16.52 20.28 25.35

22.85 22.27 20.02

20.47 21.60 22.29

20.29 27.99 22.28

Rataan 19.97

21.50

22.92

20.72

21.71

21.45

23.52

Keterangan : Perlakuan

I I1

III

= Ransum basal = Ransum basal + Kemzim = Ransum basal + Sodium pravastatin

IV

= Ransum

V VI W

= Ransum basal + Lisin + Metionin = Ransum basal + Mikroelernen = Ransum basal + Lisin + Metionin + Mikroelemen

Mikroelemen :

-

basal + Karnitin

Asam askorbat Piridoksin Niacin FeS04

Larnpiran 30. Data Karkas (%) jenis kelamin Ulangan Perlakuan 1 Perl.I1 Perl.I11 Pal.IV Perl.V Perl.V1 Perl.V1I

Jantan

1 2 3

70.48 72.86 71.77

74.59 72.21 73.50

72.34 70.51 71.53

73.62 70.68 72.25

73.64 71.05 72.45

74.15 75.60 74.98

74.81 67.12 69.02

Rataan 71.70

73.43

71.46

72.18

72.38

74.91

70.32

Rataan 71.76

70.66

70.11

70.40

71.25

70.25

71.51

Keterangan : Perlakuan

I I1

III IV V VI W Mikroelemen :

= Ransum basal = Ransum basal + Kemzim = Ransum basal + Sodium pravastatin = Ransum basal + Karnitin = Ransum basal Lisin + Metionin

+ + Mikroelemen = Ransum basal + Lisin + Metionin + Mikroelemen

= Ransum basal

-

Asam askorbat Piridoksin Niacin FeS04

Lampiran 31. Data Lemak Abdomen (%) Jenis kelamin Ulangan Perlakuan I Perl. l l Perl. I11 Perl. IV Perl. V Perl. VI Perl. VII

Rataan 2.74

2.32

2.44

2.15

2.57

Rataan 2.98

2.43

2.32

2.06

2.65

Keterangan : Perlakuan

I I1

m lv v VI vn Mikroelernen :

= Ransum basal = Ransum basal + Kemzim = Ransum basal + Sodium pravastatin = Ransum basal + Karnitin

= Ransum basal + Lisin + Metionin = Ransum basal = Ransum basal

-

+ Mikroelernen + Lisin + Metionin + Mikroelernen

Asam askorbat Piridoksin Niacin FeS0.j

Lampiran 32. Data Kolesterol Darah (mgtdl) Jenis kelamin Ulangan Perlakuan l Perl. I1 Perl. 111 Perl. IV Perl. V Perl. V1 Perl. VII Jantan

Betina

1

142

109

106

100

115

116

103

Rataan 139

110

110

97

112

113

105

1 2 3

111 115 110

116 109 111

80 81 76

108 114 114

119 123 121

111 104 106

130 123 125

Keterangan : = Ransum basal = Ransum basal = Ransum basal

+ Kemzim

+ Sodium pravastatin

= Ransum basal + Kamitin

= Ransum basal + Lisin + Metionin = Ransum basal + Mikroelemen = Ransum basal + Lisin + Metionin + Mikroelemen

Mikroelemen :

-

Asam askorbat Piridoksin Niacin FeS04

Lampiran 33.

Data LDL Darah (mgldl)

Jenis kelamin Ulangan Perlakuan I Perl. 11 Perl. 111 Perl. lV Perl. V Perl. VI Perl. VI1

Rataan 43.2

32.4

30.4

8.8

29.2

36.6

22.8

42.1 39.6 40.7

26.1 24.8 24.1

23.1 21.2 23.5

8.9 7.9 8.4

17.2 15.9 16.1

41.2 38.1 37.7

20.1 18.9 18.6

Rataan 40.8

25.0

22.6

8.4

16.4

39.0

19.2

Betina

1

2 3

Keterangan : Perlakuan

= Ransum basal = Ransum basal = Ransum basal

+ Kemzim + Sodium pravastatin

= Ransum basal

+ Kamitin

VI

= Ransum basal

VII

= Ransum basal

+ Mikroelemen + Lisin + Metionin + Mikroelemen

I

II III IV

v

Mikroelemen :

= Ransum basal + Lisin + Metionin

-

-

Asam askorbat Piridoksin Niacin FeS04

Lampiran 34. Data HDL Darah (mg/dl) Jenis kelamin Ulangan Pcrlakuan I Pal. I1 Perl. 111 Perl. IV Perl. V Perl. VI Perl. VII Jantan

1

2 3

45 48 45

Rataan 46

Betina

1 2 3

43 40 40

Rataan 41

Keterangan : Perlakuan

I

= Ransum basal

11

= Ransum basal

m

= Ransum basal

N

= Ransum = Ransum

v

VI

vn Mikroelemen :

+ Kemzim

+ Sodium pravastatin basal + Kamitin basal + Lisin + Metionin = Ransum basal + Mikroelemen = Ransum basal + Lisin + Metionin + Mikroelemen

-

Asam askorbat Piridoksin Niacin FeS04

Lampiran 35. Data Trigliserida (TG) Darah (mgldl)

Jenis kelamin Ulangan Perlakuan I Perl. I1 Perl. I11 Perl. IV Perl. V Perl. V1 Perl. VII

Betina

Rataan 159

88

86

74

82

138

79

161 155 158

105 103 101

97 96 92

75 70 68

80 75 76

145 143 135

80 76 72

Rataan 158

103

95

71

77

141

76

1 2 3

Keterangan : Perlakuan

I I1

m N v VI VII Mikroelemen :

= Ransum basal = Ransum basal + Kemzim = Ransum

basal + Sodium pravastatin

= Ransum basal + Kamitin = Ransum basal + Lisin + Metionin = Ransum

basal + Mikroelemen + Lisin + Metionin + Mikroelemen

= Ransum basal

-

-

Asam askorbat Piridoksin Niacin FeSOd

Lampiran 36. Data Usus dan Pankreas (%)

Jenis kelamin Ulangan Perlakuan l Pal. I1 Pal. 111 Pal. IV Perl. V Perl. V1 Perl. VII

Jantan

3.37 2.98 3.55

4.43 3.45 2.46

3.45 2.28 2.87

3.05 3.69 2.38

2.91 2.86 2.61

4.56 2.25 3.51

2.21 2.41 2.65

Rataan 3.30

3.45

2.87

3.04

2.79

3.44

2.42

1 2 3

4.20 4.99 3.21

4.86 4.17 3.28

4.21 3.02 3.72

3.76 5.25 2.07

5.43 3.90 2.16

4.97 3.26 4.22

3.53 4.37 2.49

Rataan 4.13

4.10

3.65

3.69

3.83

4.15

3.46

1

2 3

Betina

Keterangan : Perlakuan

I

I1 III IV V VI VII

= Ransum basal = Ransum basal + Kemzim = Ransum basal + Sodium pravastatin = Ransum

basaI + Kamitin

= Ransum

basal + Lisin + Metionin + Mikroelemen

= Ransum basal + Lisin + Metionin = Ransum basal + Mikroelemen

Mikroelemen : - Asarn askorbat - Piridoksin - Niacin - FeS04

Lampiran 37. Data Giblet (Hati, Jantung, Empedal) (%)

Jenis kelamin Ulangan

Perlakuan 1 Perl. I1 Perl. Ill Perl. lV Perl. V Perl. V1 Perl. VII

Jantan

5.56 4.52 5.14

5.55 5.15 4.54

5.31 5.87 4.54

5.53 6.66 4.19

5.73 5.23 4.52

Rataan 5.07

5.08

5.24

5.46

5.16

Rataan 5.94

6.42

5.48

6.19

5.92

1

2 3

-

6.27 4.23 5.40

5.68 6.08 5.08

5.33

5.61

5.50

5.71

Keterangan : Perlakuan

I 11 Ill

= Ransum basal = Ransum basal + Kemzim = Ransum basal

+ Sodium pravastatin

IV

= Ransum basal + Karnitin

V VI VII

= Ransum basal = Ransum basal = Ransurn basal

Mikroelemen : - Asam askorbat - Piridoksin - Niacin - FeS04

+ Lisin + Metionin + Mikroelemen + Lisin + Metionin + Mikroelemen

Lampiran 38. Data Asam Palmitat Daging (g/lOO g Asam Lemak)

Jenis kelamin Ulangan Perlakuan I Perl. I1 Perl. I11 Pwl. lV Perl. V Perl. VI Pal. V11

Jantan

Betina

2 3

3.18 3.1 1 4.01

3.10 3.08 4.12

3.49 3.46 4.17

2.29 2.18 3.11

3.37 3.27 3.27

1 2 3

4.08 3.12 4.09

3.15 3.11 4.21

3.56 3.17 4.62

2.25 2.09 3.09

3.35 3.11 3.17

Rataan 3.76

3.49

3.78

2.48

3.21

1

Keterangan : Perlakuan

I I1

= Ransum basal = Ransum basal + Kemzim

+ Sodium pravastatin + Kamitin = Ransum basal + Lisin + Metionin

IU

= Ransum basal

IV V VI VII

= Ransum basal = Ransum basal

+ Mikroelemen

= Ransum basal + Lisin + Metionin + Mikroelemen

Mikroelemen : - Asam askorbat - Piridoksin - Niacin - FeS04

Lampiran 39.

.

Data Asam Stearat Daging (g/100 g Asam Lemak)

Jenis kclamin Ulangan Perlakuan I Perl. I1 Perl. 111 Perl. lV Perl. V Perl. V1 Perl. VIl

Rataan 32.23

32.82

31.61

33.40

34.27

31.37

32.23

Rataan 32.50

32.56

32.33

33.41

34.20

31.49

32.44

Keterangan : Perlakuau

I

n III

rV V VI VII

= Ransum basal = Ransum basal + Kemzim = Ransum basal + Sodium pravastatin = Ransum basal + Karnitin

+ Lisin + Metionin + Mikroelemen = Ransum basal + Lisin + Metionin + Mikroelemen = Ransum basal

= Ransum basal

Mikroelemen : - Asarn askorbat - Piridoksin - Niacin - FeS04

Lampiran 40. Data Asam Oleat Daging (g1100 g Asam Lemak)

Jenis kelamin Ulangan Perlakuan I Perl. 11 Perl. I11 Perl. IV Perl. V Pal. V1 Pal. VI1 lantan

Betina

1 2 3

27.30 28.02 28.09

28.01 27.92 27.76

27.86 27.61 27.82

28.24 28.21 27.71

27.78 26.26 26.16

26.78 26.71 26.51

27.31 27.62 27.71

Rataan 27.80

27.90

27.76

28.05

26.73

26.67

27.55

1 2 3

26.92 28.12 28.01

27.06 27.91 28.06

27.91 27.62 27.11

28.28 28.01 26.91

27.61 28.17 28.09

26.90 26.82 26.91

28.32 27.11 28.91

Rataan 27.68

27.68

27.55

27.73

27.96

26.88

28.11

Keterangan : Perlakuan

I I1

Dl N V VI VII

= Ransum basal = Ransum basal + Kemzim = Ransum basal + Sodium pravastatin = Ransurn basal + Karnitin

+

= Ransurn basal Lisin + Metionin = Ransum basal + Mikroelemen

= Ransum basal + Lisin + Metionin + Mikroelemen

Mikroelemen : - Asam askorbat - Piridoksin - Niacin - FeS04

Lampiran 41. Data Asam Linoleat Daging (gI100 g Asam Lemak)

Jenis kelamin Ulangan Perlakuan 1 Perl. ll

Betina

Perl. 111 Perl. lV Perl. V Perl. VI Perl. V11

Rataan 29.74

29.00

30.74

30.17

1 2 3

28.76 29.68 29.72

28.72 28.67 29.68

30.75 30.25 30.06

30.29 30.09 29.06

Rataan 29.39

29.02

30.35

29.81

Keterangan : Perlakuan

I

= Ransum basal

II III N

= Ransum = Ransum

V VI VII

basal + Kemzim basal + Sodium pravastatin = Ransurn basal + Karnitin = Ransum basal + Lisin + Metionin = Ransum basal + Mikroelemen = Ransum basal + Lisin + Metionin + Mikroelemen

Mikroelemen : - Asam askorbat - Piridoksin - Niacin - FeS04

Lampiran 42. Data Asam a-Homolinolenat Daging (g1100 g Asam Lemak)

Jenis kelamin Ulangan Perlakuan I Perl. 11 Perl. 111 Perl. IV Perl. V Perl. V1 Perl. VII Jantan

Betina

1 2 3

0.92 0.87 0.96

1.09 1.04 1.09

Rataan 0.92

1.07

1 2 3

0.81 0.92 1.06

1.16 1.09 1.12

Rataan 0.93

1.12

-

-

Keterangan : Perlakuan

I 11

El IV V VI VII

= Ransum = Ransum = Ransum

basal basal + Kemzim basal + Sodium pravastatin = Ransum basal + Kamitin = Ransum basal + Lisin + Metionin = Ransum basal + Mikroelemen = Ransum basal + Lisin + Metionin + Mikroelemen

Mikroelemen : - Asam askorbat - Piridoksin - Niacin - FeS04

Lampiran 43.

.

Data Asam Eikosapentaenoat (EPA) Daging (gI100 g Asam Lemak)

Jenis kelamin Ulangan Perlakuan I Perl. 11 Perk. I11 Perl. lV Perl. V Perl. V1 Perl. VIl

Betina

Rataan 2.43

3.03

2.36

2.29

2.71

3.69

2.64

1 2 3

2.68 2.75 1.78

3.82 3.21 1.92

2.11 3.24 1.95

1.72 3.10 3.42

2.14 3.02 2.96

3.72 2.72 2.91

2.81 2.61 3.72

Rataan 2.40

2.98

2.43

2.75

2.71

3.12

3.05

Keterangan : Perlakuan

I

II Ill IV V VI W

= Ransum basal = Ransum basal + Kemzim = Ransum basal

+ Sodium pravastatin

= Ransum basal + Karnitin = Ransum basal + Lisin + Metionin = Ransum basal + Mikroelemen = Ransum basal + Lisin + Metionin + Mikroelemen

Mikroelemen : - Asam askorbat - Piridoksin - Niacin - FeS04

Lampiran 44. Data Asam Dokosapentaenoat Daging (g1100 g Asam Lemak)

,

Jmis kelamin Ulangan Perlakuan I Perl. I1 P a l . I11 Perl. IV Perl. V Perl. V1 P a l . Vll

Betina

Rataan 1.13

1.06

1.25

0.86

0.55

1.71

0.86

1.12 1.17 1.86

1.16 1.09 1.72

1.42 1.26 2.71

1.07 0.92 0.39

0.56 0.48 0.41

1.26 2.51 2.36

0.61 0.72 0.41

Rataan 1.38

1.32

1.80

0.79

0.48

2.04

0.58

1

2 3

Keterangan : Perlakuan

I

I1 Dl

N V VI VII

= Ransum basal = Ransum basal

+ Kemzim + Sodium pravastatin = Ransum basal + Karnitin = Ransum basal + Lisin + Metionin = Ransum basal + Mikroelemen = Ransum basal + Lisin + Metionin + Mikroelemen = Ransum basal

Mikroelemen : - Asam askorbat - Piridoksin - Niacin - FeS04

Lampiran 45.

.

Data Asam Dokosaheksaenoat (DHA) Daging (g1100 g Asam Lemak) Jenjs kelamin Ulangan Perlakuan l Pcrl. ll Perl. I11 Perl. lV Perl. V Perl. VI Perl. VII

Betina

1 2 3

Keterangan : Perlakuan

I

II

= Ransum basal = Ransurn basal + Kemzim

III

= Ransum basal + Sodium pravastatin

N

= Ransum

V VI VLI

= Ransum

basal + Kamitin basal + Lisin + Metionin = Ransum basal + Mikroelemen = Ransum basal + Lisin + Metionin + Mikroelernen

Mikroelemen : - Asam askorbat - Piridoksin - Niacin - FeS04

Lampiran 46. Data Asam Lemak Jenuh Daging (gI100 g Asam Lemak)

p~

.

--

Jenis kelamin Ulangan Perlakuan 1 Perl. I1 Pal. Ill Perl. lV Perl. V Pal. VI Perl. VII Jantan

Betina

1 2 3

35.89 35.03 36.07

35.99 35.79 36.98

34.86 34.81 36.28

36.47 36.20 35.12

37.94 37.44 37.33

34.68 34.18 34.05

34.52 34.68 34.72

Rataan 35.66

36.25

35.32

35.93

37.57

34.30

34.64

1 2 3

37.60 34.98 36.20

36.56 35.67 35.93

34.88 34.22 36.24

36.42 36.10 35.15

37.85 37.20 37.18

33.67 34.49 35.00

35.47 34.62 34.63

Rataan 36.26

36.05

35.11

35.89

37.41

34.39

34.91

Keterangan : Perlakuan

I 11

= Ransurn basal

= Ransum basal + Kemzim

+ Sodium pravastatin

LlI

= Ransum basal

IV

= Ransum basal

V VI VU

= Ransurn basal + Mikroelemen

+ Karnitin = Ransum basal + Lisin + Metionin = Ransum basal

Mikroelemen : - Asam askorbat - Piridoksin - Niacin - FeS04

+ Lisin + Metionin + Mikroelemen

Lampiran 47. Data Asam Lemak Tidak Jenuh Daging (g1100 g Asam Lemak)

.

Jenis kelamin Ulangan Perlakuan I Perl. I1 Perl. I11 Perl. IV Perl. V Perl. VI Perl. VII

Jantan

64.11 64.97 63.93

64.01 64.21 63.02

65.14 65.19 63.72

63.53 63.80 64.88

62.06 65.32 62.56 65.82 62.67 65.95

65.48 65.32 65.28

Rataan 64.34

63.75

64.68

64.07

62.43 65.70

65.36

1 2 3

62.40 65.02 63.80

63.44 64.33 64.07

65.12 65.78 63.76

62.15 66.33 62.80 65.51 62.82 65.00

64.53 65.38 65.37

I

= Ransum basal

LI

= Ransum basal

1

2 3

Betina

63.58 63.90 64.85

Keterangan : Perlakuan

+ Sodium pravastatin basal + Karnitin = Ransum basal + Lisin + Metionin = Ransum basal + Mikroelemen = Ransum basal + Lisin + Metionin + Mikroelemen = Ransum basal

IV V

VI VLl

+ Kemzim

= Ransum

Mikroelemen : - Asam askorbat - Piridoksin - Niacin - FeS04

Lampiran 48. Data Asam Lemak Omega-3 (n-3) Daging (&I00 g Asam Lemak)

.

Jenis kelamin Ulangan Perlakuan I Perl.I1 Perl.111 Perl.IV Perl. V Perl.VI Perl.VI1

Betina

1 2 3

5.60 6.05 4.21

6.50 6.66 4.61

5.04 6.65 3.88

3.94 4.88 8.49

I II

= Ransum basal

III IV

= Ransum basal + Sodium pravastatin

V VI VII

= Ransum basal + Lisin + Metionin

3.91 4.99 5.31

9.16 7.08 7.71

4.53 4.49 5.88

Keterangan : Perlakuan

= Ransum basal + Kemzim = Ransum basal

= Ransum basal + Mikroelemen

= Ransum basal + Lisin + Metionin + Mikroelemen

Mikroelemen : - Asam askorbat - Piridoksin - Niacin FeS0.i

-

+ Karnitin

Lampiran 49. Data Asam Lemak Omega-6 (n-6) Daging (g1100 g Asam Lemak)

.

Jenis kelamin Ulangan Perlakuan I Perl. I1 Perl. Ill Perl. IV P a l . V Perl. VI Pal. VI1

Betina

1 2 3

29.88 30.85 31.58

29.88 29.76 31.40

32.17 31.51 32.77

31.36 31.01 29.45

30.63 29.64 29.42

30.27 31.61 30.38

31.68 33.78 30.58

I I1

= Ransum basal

IV

= Ransum basal + Karnitin

V VI VII

= Ransum basal + Lisin + Metionin = Ransum basal + Mikroelemen = Ransum basal + Lisin + Metionin + Mikroelemen

Keterangan :

Perlakuan

= Ransum basal + Kemzim = Ransum basal + Sodium pravastatin

Mikroelemen : - Asam askorbat - Piridoksin - Niacin - FeS04