BIOREMIDIATION PENGANTAR BIOTEKNOLOGI
DEFINITION
PHYTOREMEDIATION
OUTLINE BIOREMEDIATION TECHNIQUE
CASE STUDY
What is BIOREMEDIATION
The technology used to speed up the natural processes of waste degradation and recycling
Use of naturally occurring microorganism such as bacteria, fungi, and yeast to degrade pollutants or hazardous substances in soil, water and air into nontoxic or less toxic substances in order to return the environment to its original natural condition
TRIPLE CORNERS PROSESS INORGANIC ORGANIC
POLLUTANS
ENVIRONMENT SOIL WATER AIR
ORGANISM MICROORGANISM
Municipal water well
po nd
Leaking petroleum tank Ocean
Water table Fresh water
Saline Water
septic tank leakage
Lateral intrusion of saline water
Infiltration of pesticides and fertilizers from farmlands Brine leakage from ruptured well casing
Municipal landfill
Contaminated shallow well
Accidental fuel spill Confining Unit
Confining Unit Abandoned oil well
Leakage from hazardous waste site
Deep Aquifer
PLANTS (Phytoremediation) ENZYME
MICROORGANISM 1- Isolation of the microorganism
2- Purification of the obtained isolates 3- Identification of the microbial isolate 4- Optimization of the biodegradation conditions 5- Determination of the biodegradation efficiency 6- Identification of the biodegradation products. 7- Cell or enzyme immobilization. 8- Enzyme identification.
GMO APPLICATION FOR BIOREMIDIATION
Deinococcus radiodurans organisme paling radioresistant dimodifikasi untuk dapat mencerna toluene dan ionic mercury dari limbah dg kandunga radioactive nuclear yang tinggi
DEFINITION
PHYTOREMEDIATION
OUTLINE BIOREMEDIATION TECHNIQUE
CASE STUDY
PHYTOREMEDIATION
Phytoremediation is use of plants (HYPERACCUMULATOR PLANT) for accumulation, removal or conversion of pollutants. PENGURAIAN ZAT KONTAMINAN
TRANSPIRASI ZAT KONTAMINAN DLM BENTUK MENJADI LARUTAN TERURAI TDK BERBAHAYA
PENEMPELAN ZAT KONTAMINAN PADA AKAR
TUMBUHAN MENARIK ZAT KONTAMINAN, BERAKUMULASI DI DAUN
(Rhyzodegradation)PENGURAIAN ZAT KONTAMINAN OLEH AKTIVITAS MO
HYPERACCUMULATOR PLANT
Can adsorb more than 10.000 ppm for Mn, Zn, Ni
> 1.000 ppm for Cu and Se
> 100 ppm for Cd, Cr, Pb and Co
METAL CONTAMNANTS IN THE SOIL are ABSORBED by the roots (UPTAKE) move in to shoot (TRANSLOCATION)
are STORED in the shoot (ACCUMULATION)
Thiaspi caerulescens
Nicotina tabbacum
Berkheya sp
Alyssum murale
FITOREMIDIATION BENEFIT
LOW COST OPERATIONAL
DESCRESE CONTAMINANT POLLUTANS NATURALLY
CONTAMINATED PLANT CAN USED AS FUEL
MYCOREMEDIATION
USE FUNGGI AS DECONTAMINATION AREAL
USED FUNGAL MYCELIA
EX: DIESEL OIL CONTAMINATED AREA INOCULATE WITH OYSTER FUNGI. CAN CHANGE PAH (polycyclic aromatic hydrocarbon) CO2 + H2O
DEFINITION
PHYTOREMEDIATION
OUTLINE BIOREMEDIATION TECHNIQUE
CASE STUDY
IN SITU BIOREMEDIATION
SOIL BIOREMIDIATION (IN SITU PROCESSING)
BIOVENTING
Pemompaan udara dan Nutrisi
AIR SPARGING Pememompaan udara untuk meningkatkan aktifitas degradasi oleh mikroba
SOIL BIOREMIDIATION (EX-SITU PROCESSING)
Tanah terkontaminasi diangkat ke dan diperlakukan di permukaan
SOIL BIOREMIDIATION (EX-SITU PROCESSING)
SLURRY PHASE menggunakan bioreaktor
SOIL BIOREMIDIATION (COMBINATION IN SITU & EX-SITU PROCESSING)
WATER AND GAS BIOREMIDIATION
Biofiltration is a process, in which, microorganisms supported on inert materials are used to degrade organic pollutants for air, gas and water bioremediation.
Types of biofilters: 1- Bioscrubbers. 2- Biotrickling filters. 3- Slow sand or carbon filters.
Bioscrubber filters
DEFINITION
PHYTOREMEDIATION
OUTLINE BIOREMEDIATION TECHNIQUE
CASE STUDY
Case Study
Sebuah perusahaan POM BENSIN terbesar di Las Vegas, USA sedang menhadapi masalah serius. Tangki yang ditanam dalam tanah sedalam 10 meter mengalami kebocoran.
Jika Anda sebagai Konsultan, diminta untuk menyelesaikan masalah perusahaan POM BENSIN, Las Vegas, USA tersebut. Apa yang akan anda lakukan ?
1. THE EXXON VALDEZ OIL SPILS 2. TRAGEDI MINAMATA 3. KASUS LOVE CANAL (AMERIKA SERIKAT) 4. KASUS KABUT DIOXIN DI SEVESO (ITALIA) 5. PT. NEWMONT MINAHASA RAYA PENCEMAR TELUK BUYAT
• JELASKAN KASUS PENCEMARAN TERSEBUT SERTA DAMPAK YANG DITIMBULKAN • JIKA ANDA SEBAGAI KONSULTAN BIDANG LINGKUNGAN SOLUSI APAKAH YANG AKAN BERIKAN UNTUK MENGATASI MASALAH TERSEBUT (IDENTIFIKASI POLLUTAN, ENVIRONMENT DAN ORGANISM)
