Gizi dan Kesehatan LAPORAN PENELITIAN HIBAH BERSAING TAHUN ANGGARAN 2011
Pengaruh Ekstrak Air Cincau Hitam (Mesona Palustris BL) terhadap Ekspresi Interferon Gama Dan Aktivasi Immunosurveillance serta Efek Sitotoksiknya pada Kultur Sel Hela
Dr. Ir. Tri Dewanti Widyaningsih, M.Kes Jaya Mahar STP Novita Wijayanti STP
Dibiayai oleh Direktorat Jenderal Pendidian Tinggi, Kementerian Pendidikan Nasional, melalui DIPA Universitas Brawijaya REV.1 Nomor: 0636/023-04.2.16/15/2011 R, tanggal 30 Maret 2011 dan berdasarkan surat dari DP2M Dikti Nomor: 121/D3/PL/2011 tanggal 7 Februari 2011
UNIVERSITAS BRAWIJAYA NOPEMBER 2011
RINGKASAN Tanaman cincau hitam (Mesona palustris BL) yang merupakan bahan baku gel cincau hitam, selain sebagai bahan pangan sudah lama dipercaya berkhasiat sebagai obat ”panas dalam” maupun penyakit lainnya. Dilaporkan bahwa beberapa efek farmasi herbal dapat dikaitkan dengan aktivitas antioksidan Tujuan penelitian ini adalah untuk menentukan pengaruh ekstrak air cincau hitam terhadap ekspressi/kadar Interferon gama dan aktifitas sel NK, Sel sitotoksik dan makrofag secara in vivo dan menentukan efek sitotoksiknya dengan kultur sel Hela secara in vitro. Tahapan penelitian dalam 2 tahun. Tahun 1 : Skrining fitokimia ekstrak cincau hitam dan Pengaruh Ekstrak Air Cincau Hitam (Mesona Palustris BL) terhadap Ekspresi Interferon Gama dan Aktivasi Immun surveillance dalam darah mencit yaitu peningkatan aktivitas sel Natural Killer, Sel T sitotoksik dan makrofag dengan metode flowcytometri. Penelitian tahun kedua ini dilakukan uji sitotoksisitas secara in vitro ekstrak air, ekstrak etanol dan ekstrak etil asetat cincau hitam terhadap sel Hela menggunakan metoda MTT (3-4(4,5-dimetil-diazol-2-il)-2,5-diphenil tetrazolinum bromid) dengan 10 tingkat konsentrasi ekstrak. Hasil Penelitian, ekstrak air, etanol dan etil asetat dari cincau hitam menunjukkan potensi bioaktivitas sebagai antikanker pada sel Hela. Aktivitas ketiga ekstrak tersebut tidak berbeda nyata. Ekstrak air cincau hitam pada konsentrasi yang rendah ( sampai 156,25 µg/ml) menunjukkan aktivitas antikanker yang lebih tinggi dibandingkan ekstrak etanol dan etil asetat. LC50 ekstrak air cincau hitam adalah 296,6 µg/ml, ekstrak etanol cincau hitam 246,0 µg/ml dan ekstrak etil asetat cincau hitam 282,96 µg/ml.
SUMMARY
Black cincau (Mesona palustris BL) or Grass black jelly is a traditional Indonesian food that has been used as a folk medicine and is effective against heatshock,hypertension,diabetes and muscle and joint pains. It was reported that some of pharmaceutical effect of herbs could be related to their possible antioxidant activities The purpose of this study was to determine the effect of water extract of black of cincau (Mesona palustris BL) expression of interferon gamma and NK cell activity, Cell cytotoxic and macrophages in vivo and determine the cytotoxic effects of mice with Hela cell cultures in vitro. Stages of the study within 2 years. Year 1: Phytochemical screening of extracts of black cincau (Mesona palustris BL) and Effect of Water Extracts of black cincau against interferon gamma expression and activation of immune surveillance in the blood of mice is increased activity of Natural Killer cells, cytotoxic T cells and macrophages with flowcytometri method. Second year of the studies to determine the bioactivity of black cincau extract against Hela cell. Cytotoxicity assays was conducted using method the MTT with 10 levels of extract concentration. The results of cytotoxicity assays showed the water extract, ethanol extract and the ethyl acetate extract of black cincau has anticancer activity against Hela cell lines. The third activity of black cincau extracts was not significantly different. Water extracts of black cincau at low concentrations (up to 156,25 µg/ml) showed higher anticancer activity than extracts ethanol and ethyl acetate. LC50 water extract of black cincau is 296,6 µg/ml, ethanol extract of black cincau is 246,0 µg/ml and ethyl acetate extract of black cincau is 282,96 µg/ml
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