131
DAFTAR PUSTAKA
Agrios GN. 1997. Plant Pathology. San Diego: Academic Press, Inc. Ahloowalia BS. 1986. Limitation to the use of somaclonal variation in crop improvement. Di dalam: Semal J, editor. Somaclonal Variation and Crop Improvement . New York: Martinus Nijhoff Pub. hlm. 14-27 Ahloowalia BS, Maluszynski M. 2001. Induced mutation - A new paradigm in plant breeding. Euphytica 118: 167-173. Ahloowalia BS, Maluszynski M, Nichterlein K. 2004. Global impact of mutationderived varieties. Euphytica 135:187-204. Ahmed KZ, Mesterhazy A, Sagi F. 1991. In vitro techniques for selecting wheat (Triticum aestivum L.) for Fusarium-resistance. I. double- layer culture technique. Euphytica 57:251-257 Ahmed KZ, Mesterhazy A, Bartok T, Sagi F. 1996. In vitro techniques for selecting wheat (Triticum aestivum L.) for Fusarium-resistance II. Culture filtrate technique and inheritance of Fusarium-resistance in the somaclones. Euphytica 91: 341-349 Aragon TC. 2000. Fiber crops programe area research planning and prioritization. Phillipine Institute for Development Studies. http://www.pids.gov.ph [28 Juni 2006] Arumingtyas EL, Indriyani S. 2005. Induksi variabilitas genetik percabangan tanaman kenaf (Hibiscus cannabinus L.) dengan mutagen kimia Ethyl Methane Sulfonate (EMS). Natural Jurnal 8 (2): 24-28 Bacon CW, Porter JK, Norred WP, Leslie JF. 1996. Production of Fusaric acid by Fusarium species. App. Env. Microbiol 62(11): 4039-4043. Bastasa GN, Baliad AA. 2005. Biological control of Fusarium wilt of abaka (Fusarium oxysporum) with Trichoderma and yeast. Philippines J Crops Sci 30:29-37. Basuki S. 2003. Identification of DNA markers and recombinations events in the vinicity of the Fusarium oxysporum f.sp. licopersici resistance gene I-3 of tomato (Lycopersicon esculentum) [Thesis]. Brisbane: School of Land & Food Sciences and School of Molecular & Microbiology Sciences, The University of Queensland.
132
Ben-Yephet Y, Reuven M, Zviebil A, Shtienberg D. 1996. Effects of initial inoculum and cultivar resistance on incidence of Fusarium wilt and population densities of Fusarium oxysporum F.sp. dianthi on carnation and in soil. Phytopathology 86:751-756. Berger J. 1969. The world’s major fibres crops. Their cultivation and manuring. Zurich: Centre d’Etude de l’Azote. BI. 2003. Model Kelayakan Proyek Kemitraan Terpadu perkebunan Pisang Abaka. http://www.bi.go.id/sipuk/lm/ind/pisang-ABACA [18 April 2003] Bird RMcK, Neuffer MG. 1987. Induced mutation in maize. Plant breeding Reviews 5:139-180 Borras O, Santos R, Matos AP, Cabral RS, Arzola M. 2001. A first attempt to use a Fusarium subglutinans culture filtrate for the selection of pineapple cultivars resistant to fusariose disease. Plant Breeding 120: 435-438. Bouizgarne B, Bouteau HE, Frankart C, Reboutier D, Madiona K, Pennarun AM, Monestiez M, Trouverie J, Amiar Z, Briand J, Brault M, Rona JP, Ouhdouch Y, Hadrami IE, Bouteau F. 2006. Early physiological responses of Arabidopsis thaliana cells to fusaric acid: toxic and signalling effects. New Phytologist 169:209-218. Burge Y, Katzir N, Tzuri G, Portnoy V, Saar U, Shriber S, Perl- Treves R, Cohen R. 2003. Variation in the resistance of melon genotypes to Fusarium oxysporum f.sp. melonis race 1 determine by inoculation tests and molecular markers. Plant Pathol 52:204-211. Cachinero JM, Hervas A, Jimenez-Diaz RM, Tena M. 2002. Plant defence reactions against Fusarium wilt in chickpea ind uced by incopatible race 0 of Fusarium oxysporum f.sp. ciceris and non- host isolates of F. Oxysporum. Plant Pathol 51:765-776. Cai T, Butler LG. 1996. Somaclonal variation in sorghum. Di dalam: Bajaj YPS, editor. Di dalam: Biotechnology in Agriculture and Forestry 36: Somaclonal variation in crop improvement II. Springer. hlm. 81-106. Chen M, Choi Y, Voytas DF, Rodermel S. 2000. Mutations in the Arabidopsis VAR2 locus cause leaf variegation due to the loss of chloroplast FtsH protease. The Plant Journal 22(4): 303-313 Damayanti F. 2002. Seleksi in vitro untuk ketahanan terhadap penyakit layu Fusarium pada tanaman abaka (Musa textilis Nee). [Tesis]. Bogor: Program Pascasarjana, Institut Pertanian Bogor.
133
Damayanti F. 2004. Seleksi in vitro tanaman abaka (Musa textilis Nee) dengan filtrat Fusarium oxysporum untuk ketahanan terhadap penyakit layu Fusarium. Bioscientiae 1(2):11-22. Del-Rosario AG, Zamora AB. 1988. Plant tissue culture research in the Philippines. The Philippine Agric. Special Issue:43-61 Dempsey JM. 1963. Long vegetable fiber development in South Vietnam and other Asian Countries 1957-1962. Saigon: USOM. Di Pietro A, Madrid MP, Caracuel Z, Delgado-Jarana J, Roncero MIG. 2003. Fusarium oxysporum: exploring the molecular arsenal of a vascular wilt fungus. Mol Plant Pathol 4:315-325. Djatnika I, Hermanto C, Eliza. 2003. Pengendalian hayati layu Fusarium pada tanaman pisang dengan Pseudomonas fluorescens dan Gliocladium sp. J Hort 13:205-211. Durbin RD. 1983. Future Prospect in Toxin Research. Di dalam: Daly, Deverall, editor. Toxins and Plant Pathogenesis. Sydney: Academic Press. hlm 159177. Duryatmo S, Dasoeki R. 1999. Pisang abaca bahan baku penghasil uang. Trubus 30 (351):48-49. Epp D. 1987. Somaclonal variation in banana: a case study with Fusarium wilt. Di dalam: Persley GJ, De Langhe EA, editor. Banana and Plantain Breeding Strategies. Canbera: ACIAR Publ, hlm140-150. Fadel F, Wenzei G. 1993. In vitro selection for tolerance to Fusarium in F1 microspore populations of wheat. Plant Breeding 110: 89-95 Gichner T, Stavevra DA, van Breusegem F. 2001. O-Phenylenediamine- induced DNA damage and mutagenicity in tobacco seedlings is light-dependent. Mutation Research 495: 117-125. Gichner T. 2003. Differential genotoxicity of ethyl methanesulphonate, N-ethylN-nitrosourea and maleic hydrazide in tobacco seedlings based on data of the Comet assay and two recombination assay. Mutation Research 538: 171179 Goodman RN, Kiraly Z, Wood KR. 1986. The biochemistry and physiology of plant disease. Missouri: The University of Missouri Press. Gowda MVC, Nadaf HL, Sheshagiri R. 1996. The role of mutation in intraspecific differentiation of groundnut (Arachis hypogaea L). Euphytica 90:105-113
134
Hadipoentyanti E, Ratnadewi D, Solihat L. 2001. Variabilitas genetik berbagai varietas abaka (Musa textilis Nee) dan kerabat liar melalui analisis RAPD. Zuriat 12:93-106. Harborne JB. 1988. Introduction to ecological biochemistry. Edisi ke-tiga. London: Academic Press Limited. 356 hlm. He CY, Hsiang T, Wolyn DJ. 2002. Induction of systemic disease resistance and pathogen defence responses in Asparagus officinalis inoculated with nonpathogenic strains of Fusarium oxysporum. Plant Pathol 51:225-230. Heliyanto B, Marjani. Setyo-Budi U, Sudjindro, Kangiden DI. 1995. Eksplorasi plasma nutfah abaca di daerah Lampung Selatan. Bul. Tembakau dan Serat 4: 7-9 Heyne K. 1987. Tumbuhan Berguna Indonesia I. Jakarta: Yayasan Sarana Warna Jaya. Hidalgo OB, Santos R, Tussel RT, de Matos AP, Cabral RS, Arzola M, Perez MC. 1999. Phytotoxity of Fusarium subglutinans culture filtrates on in vitro plantlets and calli of resistant and susceptible pineapple (Ananas comosus). Plant Pathol. 48: 756-758. Hilario A. 2006. The abaca republic. Philippines J. Crop Sci. 31(2): 01-02. Hilman I, Toruan-Mathius N. 2001. Budi daya dan prospek pengembangan abaka. Jakarta: Penebar Swadaya. Hwang SC, Ko WH. 1987. Somaclonal variation of bananas and screening for resistance to Fusarium wilt. Di dalam: Persley GJ, De Langhe EA, editor. Banana and plantain breeding strategies. Canberra: ACIAR. hlm 151-156. Hwang SC, Ko WH. 2004. Cavendish banana cultivars resistant to Fusarium wilt acquired through somaclonal variation in Taiwan. Plant Disease 88(6): 580588. Hutagalung L. 2002. Status penyakit layu dan penyakit kerdil pisang di propinsi Lampung. Makalah Seminar Pengendalian Penyakit Layu Pisang. Padang, 22-23 Oktober 2002. 18 hlm. Inayati A. 2003. Seleksi ketahanan in vitro plantlet vanili terhadap Fusarium oxysporum f.sp vanillae menggunakan teknik double layer, kultur filtrat dan asam fusarat. [Tesis]. Yogyakarta: Program Pascasarjana. Universitas Gadjah Mada. Ishak, Dwimahyani I. 2005. Evaluasi pertumbuhan dan ketahanan galur mutan pisang terhadap F. oxysporum fsp. Cubense (FOC). J Stigma 13:26-30.
135
Jayasankar S, Li Z, Gray DJ. 2000. In-vitro selection of Vitis vinifera ‘Chardonnay’ with Elsinoe ampelina culture filtrate is accompanied by fungal resistance and enhanced secretion of chitinase. Planta 211: 200-208. Jin H, Hartman GL, Huang YH, Nickell CD, Widholm JM. 1996. Regeneration of soybean plants from embryogenic suspension cultures treated with toxic culture filtrate of Fusarium solani and screening of regenerants for resistance. Phytopathology 86 (7): 714-718. Joseph R, Yeoh H-H, Loh C-S. 2004. Induced mutations in cassava using somatic embryos and the identification of mutant plants with altered starch yield and composition. Plant Cell Rep 23(1-2):91-98. Karp A. 1995. Somaclonal variation as a tool for crop improvement. Euphytica 85:295-302. Kumar PS, Matur, VL. 2004. Chromosomal instability in callus culture of Pisum sativum. Plant Cell Tiss. Org. Cult. 78: 267-271. Leal MR, Maribona RH, Rutz A, Kornsva S, Canales E, Dinkova TD, Izquierdo F, Coro O, Rizo D. 1996. Somaclonal variation as a source of resistance to eyespot disease of sugarcane. Plant Breeding 115:37-42. Li S, Hartman GL, Widholm JM. 1999. Viability staining of soybean suspensioncultured cells and a seedling stem cutting assay to evaluate phytotoxicity of Fusarium solani f.sp. glycines culture filtrates. Plant Cell Rep. 18(5): 375380. Mak C, Ho YW, Liew KW, Asif JM. 2004a. Biotechnology and in vitro mutagenesis for banana improvement. Di dalam: Jain SM, Swennen R, editor. Banana Improvement: Cellular, Molecular Biology, and Induced Mutations. Enfield: Sci Pub, Inc. hlm. 51-68. Mak C, Mohamed AA, Liew KW, Ho YW. 2004b. Early screening technique for Fusarium wilt resistance in banana micropropagated plants. Di dalam: Jain SM, Swennen R, editor. Banana Improvement: Cellular, Molecular Biology, and Induced Mutations. Enfield: Sci Publ, Inc. hlm. 11-20 Maluszynski M, Ahloowalia BS, Sigurbjörnsson B. 1995. Application of in vivo and in vitro mutation techniques for crop improvement. Euphytica 85: 303315. Mangal M, Sharma DR. 2002. In vitro mutagenesis and cell selection for the induction of black rot resistance in cauliflower. J. Hort Sci Biotech. 77 (3): 268-272. Mante S, Tepper HB. 1983. Propagation of Musa textilis Nee plants from apical meristem slices in vitro. Plant Cell Tiss. Org. Cult. 2:151-159.
136
Mariska I, Sukmadjaja D. 2003. Perbanyakan bibit abaka melalui kultur jaringan. Bogor: Balitbiogen. 14 hlm. Matsumoto K, Barbosa ML, Souza LAC, Teixeira. 1995. Race 1 fusarium wilt tolerance on banana plants selected by fusaric acid. Euphytica 84:67-71 Montgomery B. 1954. The leaf fibers. Di dalam Mauersberger HR, editor. Textile fibers. Their physical, microscopic, and chemical properties. New York: John Willey & Sons Inc. hlm 360-391 Morpurgo R, Lopato SV, Afza R, Novak FJ. 1994. Selection parameters for resistance to Fusarium oxysporum f.sp cubense race 1 and 4 on diploid banana (Musa acuminata Colla). Euphytica 75:121-129 Murashige I, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant 159: 473-497 Nagatomi S. 1996. A new approach of radiation breeding toward improvement of disease resistance in crops. Proc. Seminar on Integrated Control on Main Diseases of Industrial Crops. Bogor, 13-14 March 1996. RISMC-JICA. hlm 16-24 Nasir N, Jumjunidang, Eliesti F, dab Meldia Y. 2003. Penyakit layu Panama pada pisang: observasi ras 4 Fusarium oxysporum f.sp. cubense di Jawa Barat. J. Hort 13:269-275. Nasir N, Jumjunidang. 2003. Karakterisasi ras Fusarium oxysporum f.sp. cubense dengan metode vegetative compatibility group test dan identifikasi kultivar pisang yang terserang. J. Hort. 13(4): 276-284. Nur N. 1957. Observasi pada Musa textilis Nee. Bagian pertama: Catatan mengenai biologi bunga. Teknik Pertanian VI(11/12):391-473. Orlando R, Magro P, Rugini E. 1997. Pectic enzymes as a selective pressure tool for in vitro recovery of strawberry plants with fungal disease resistance. Plant Cell Rep. 16:272-276 Ploetz RC, Zentmyer GA, Nishujima WT, Rohrbach KG, Ohr HD. 1994. Compendium of tropical fruit diseases. Minnesota: ADS Press. Ploetz RC. 2000. Banana disease: a classic and destructive disease of banana. www.plantmanagementnetwork.org. [23 Februari 2006]. Pratt RG. 1996. Screening for resistance to Sclerotinia trifoliarum in alfalfa by inoculation of excised leaf tissue. Phytopathology 86:923-928. Purseglove JW. 1983. Tropical Crops Monocotyledons. Britain: ELBS and Longman Group Ltd. hlm 337-384.
137
Rademacher W. 1994. Gibberellin formation in microorganisms. Plant Growth Regul. 15: 303-314. Rahajeng W. 2006. Interaksi genotipe klon-klon abaka (Musa textilis Nee) dengan beberapa komposisi media pada kultur in vitro. [Skripsi]. Malang: Fakultas Pertanian, Universitas Brawijaya. Remotti PC. 1996. The role of fusaric acid in the Fusarium-Gladiolus interaction and its application in in vitro selection for resistance breeding [Disertasi]. Nijmegen: de Katholieke Universiteit Nijmegen. Remotti PC, Loffler HJM. 1996. The involvement of fusaric acid in the bulb-rot of Gladiolus. J Phytopathol 144:405-411 Remotti PC, Loffler HJM, Loten-Doting LV. 1997. Selection of cell lines and regeneration of plants resistant to fusaric acid from Gladiolus x grandiflorus c.v. ‘Peter Pear’. Euphytica 96:237-245. Roux N, Toloza A, Dolezel J, Swennen R, Lepoivre P, Zapata-Arias FJ. 1999. Usefulness of embriogenic cell suspension for the induction and selection of mutants in Musa spp. Promusa 4:18-19 Roux NS. 2004. Mutation induction in Musa-review. Di dalam: Jain SM, Swennen R, editor. Banana Improvement: Cellular, Molecular Biology, and Induced Mutations. Enfield: Sci Pub, Inc. hlm. 21-29. Sakamoto W, Tamura T, Hanba-Tomita Y, Sodmergen, Murata M. 2002. The VAR1 locus of Arabidopsis encodes a chloroplastic FtsH and is responsible for leaf variegation in mutant alleles. Genes to Cell 7:769-780. Sakamoto JM, Gordon TR. 2006. Factors influencing infection of mechanical wounds by Fusarium cincinatum on Monterey pines (Pinus radiata). Plant Pathol 55:130-136. Salerno MI, Gianinazzi S, Gianinazzi-Pearson V. 2000. Effects on growth and comparison of root tissue colonization pattern of Eucaliptus viminalis by pathogenic and non-pathogenic strains of Fusarium oxysporum. New Pythol. 146:317-324. Scala A, Bettini P, Buiatti M, Bogani P, Pellegrini G, Tognoni F. 1985. TomatoFusarium oxysporum f.sp. lycopersici interaction: “in vitro” analysis of several possible pathogenic factors. Phytopath Z, 113:90-94. Schierholt A, Rucker B, Becker HC. 2001. Inheritance of high oleic acid mutations in winter oilseed rape (Brasica napus L.). Crop Sci. 41: 14441449.
138
Semal J, Lepoivre P. 1990. Application of tissue culture variability to crop improvement. Di dalam: Bhojwani SS, editor. Developments in Crop Science 19. Plant Tissue Culture: Applications and Limitations. Amsterdam: Elsevier. hlm 301-315. Semangun H. 1996. Pengantar Ilmu Penyakit Tumbuhan. Ed ke-3. Yogyakarta: Universitas Gadjah Mada Press. Setyo-Budi U, Sudjindro, Purwati RD. 2001. Status plasma nutfah abaca: “Menyongsong program agribisnis abaca di Indonesia”. Di dalam: Kasno A et al., editor. Kontribusi Pemuliaan dalam Inovasi Teknologi Ramah Lingkungan. Malang, 28 Agustus 2001. Bandung: PERIPI. hlm 254-258. Skirvin RM, McPheeters KD, Norton M. 1994. Sources and frequency of somaclonal variation. Hort. Sci. 29(11): 1232-1237 Spasibionek S. 2006. New mutants of winter rapeseed (Brasica napus L.) with changed fatty acid composition. Plant Breeding 125(3): 259-267 Strosse H, Van den Houwe I, Panis B. 2004. Banana cell and tissue culturereview. Di dalam: Jain SM, Swennen R, editor. Banana Improvement: Cellular, Molecular Biology, and Induced Mutations. Enfield: Sci Pub, Inc. hlm. 1-10. Sudjindro. 1999. Pemanfaatan plasma nutfah dalam usaha pengembangan tanaman abaca untuk mendukung industri strategis. Makalah pada Seminar dan Lokakarya Nasional Peluang dan Potensi Serat Abaca sebagai Komoditi Ekspor Prospektif dan Pemberdayaan Ekonomi Rakyat, Jakarta: 15 September 1999. Svabova L, Lebeda A. 2005. In vitro selection for improved plant resistance to toxin-producing pathogens. J Phytopathol 153:52-64. Tabora PC, Carlos JT. 1978. Cultivation species and varieties and relatives. Intern Document Center on Abaca. Los Banos: UPLB Libr. Thakur M, Sharma DR, Sharma SK. 2002. In vitro selection and regene ration of carnation (Dianthus caryophyllus L) plants resistant to culture filtrate of Fusarium oxysporum f.sp. dianthi. Plant Cell Rep 20:825-828. Thrane U. 2001. Developments in the taxonomy of Fusarium species based on secondary metabolites. Di dalam: Sumerell BA, Leslie JF, Backhouse D, Bryden WL, Burgess LW, editor. Fusarium Paul E. Nelson Memorial Symposium. Minnesota: APS Press. hlm 29-49. Toyoda H, Tanaka N, Hirai T. 1984a. Effects of the culture filtrate of Fusarium oxysporum f.sp. Lycopersici on tomato callus growth and the selection of resistant callus cells to the filtrate. Ann. Phytopath Soc. Japan 50 (1): 53-62
139
Toyoda H, Hayashi H, Yamamoto K. 1984b. Selection of resistant tomato calli to fusaric acid. Ann. Phytopath Soc. Japan 50 (4): 538-540 Torssell KBG. 1983. Natural Product Chemistry. A mechanistic and biosynthetic approach to secondary metabolism. Chichester: John Willey & Son. 401 hlm. Triyanto HS, Muliah, Edi M. 1982. Batang abaka (Musa textilis Nee) sebagai bahan baku kertas. Berita Selulosa 18:27. van Harten AM. 1998. Mutation Breeding: theory and practical application. Cambridge: Cambridge University Press. Villareal RL. 1988. Breeding of horticulural crops in the Philippines. The Philippine Agric. Special issue:21-35 Villordon AQ. 2003. The abaca, a Philippine native. http://www.Mozcom.com/ SCF/pv/abaca.html [18 April 2003] Wardiyati T. 1999. Tanaman abaca sebagai alternatif pengembangan agroindustri di Indonesia. Makalah pada Seminar dan Lokakarya Nasional Peluang dan Potensi Serat Abaca sebagai Komoditi Ekspor Prospektif dan Pemberdayaan Ekonomi Rakyat, 15 September 1999, Jakarta. Wibowo A, Suryanti, Sumardiyono C. 2001. Patogenesis 6 isolat Fusarium oxysporum f.sp. cubense penyebab penyakit layu fusarium pada pisang. Didalam: Prosiding Kongres Nasional XVI dan Seminar Ilmiah. Bogor, 2224 Agustus 2001. Bogor: Perhimpunan Fitopatologi Indonesia, hlm 254259. Widoretno W. Harran S, Sudarsono. 2003. Keragaman karakter kualitatif dan kuantitatif pada populasi tanaman somaklon kedelai dari embrio somatik hasil seleksi in vitro. Hayati 10:110-117. Yusnita, Sudarsono. 2004. Metode inokulasi dan reaksi ketahanan 30 genotipe kacang tanah terhadap penyakit busuk batang Sclerotium. Hayati 11:53-58. Yusnita, Widodo, Sudarsono. 2005. In vitro selection of peanut somatic embryos on medium containing culture filtrate of Sclerotium rolfsii and plantlet regeneration. Hayati 12(2): 50-56. Yunus A, 2000. Pengaruh ekstrak Fusarium moniliforme terhadap pertumbuhan dan resistensi tanaman tebu terhadap penyakit pokahbung. Agrosains 2 (1):1-9.
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Lampiran 1. Sebaran nilai dan jumlah klon berdasarkan peubah panjang daun, lebar daun dan rasio panjang/lebar daun tanaman di lapangan diantara populasi abaka klon Tangongon varian yang diregenerasikan dari kalus embriogen dengan perlakuan berbagai konsentrasi EMS (0%, 0.3%, 0.4%, 0.5%, dan 0.6%), pada 16 bulan setelah tanam. 60 0.4%
0.5%
0.6%
Klon Tangongon - Panjang daun
0%
6 4
4
2
8
14
27
28
11
20
24 22
21
40
7 9
Jumlah klon
0.3%
0 <89.3
<135.9
<182.6
>182.6
Kisaran panjang daun (cm) 60
Jumlah klon
0.3%
0.4%
0.5%
0.6%
Klon Tangongon - Lebar daun
0%
40 26
25 23 20
15 4 2
1 3
20
18
8 10
10
10 5 4
3
0 <26.6
<39.1
<51.7
>51.7
Kisaran lebar daun (cm)
60
Jumlah klon
0.3%
0.4%
0.5%
0.6%
Klon Tangongon - Rasio P/L daun
0%
40 31 22 20
12
29 25 15
14
5
21 8
2
3
0 <2.54
<3.28 <4.03 Kisaran rasio panjang/lebar daun
>4.03
141
Lampiran 2. Sebaran nilai dan jumlah klon berdasarkan peubah diameter batang, bobot batang dan jumlah anakan tanaman di lapangan diantara populasi abaka klon Tangongon varian yang diregenerasikan dari kalus embriogen dengan perlakuan berbagai konsentrasi EMS (0%, 0.3%, 0.4%, 0.5%, dan 0.6%), pada 16 bulan setelah tanam.
60
Jumlah klon
0.3%
0.4%
0.5%
0.6%
Klon Tangongon - Diameter batang
0%
40 20
18
20
18
12
10 1
27
26
24
8
5
4
8
5
1
0 <19.8
<30.4 <41.1 Kisaran diameter batang (cm)
>41.1
60 Klon Tangongon - Bobot batang 0.4%
0.5%
0.6%
0%
17
6 1
1
2
2
7
8
14
20
16
16
20 25
26
40
9 10
Jumlah klon
0.3%
0 <3345
<6440
<9535
>9535
Kisaran bobot batang (g/tanaman)
60 0.4%
0.5%
0.6%
0%
Klon Tangongon - Jumlah anakan
24 21 29 6 7 6 4 8
10 3
7
5
20
18
25
40
15
Jumlah klon
0.3%
0 <3.75
<6.25
<9.25
Kisaran jumlah anakan
>9.25
142
Lampiran 3. Sebaran nilai dan jumlah klon berdasarkan peubah jumlah pelepah, kekuatan serat, rendemen serat dari batang, dan dari pelepah tanaman di lapangan diantara populasi abaka klon Tangongon varian yang diregenerasikan dari kalus embriogen dengan perlakuan berbagai konsentrasi EMS (0%, 0.3%, 0.4%, 0.5%, dan 0.6%), pada 16 bulan setelah tanam. 60 Klon Tangongon - Jumlah pelepah
0.4%
0.5%
0.6%
0%
18
2
1
8 1
3
6 2
1
20
25 13
11
19
18
20
23
40
9
Jumlah klon
0.3%
0 <5
<8
<11
>11
Kisaran jumlah pelepah 60
0.4%
0.5%
0.6%
0%
5
3
1
2
9
9
11
18
20 14
15
13
16
16
20
16
40
7
Jumlah klon
Klon Tangongon - Kekuatan serat
0.3%
0 <30.67
<36.03
<41.38
>41.38
Kisaran kekuatan serat (g/tex)
60
0.4%
0.5%
0.6%
0%
9 2
1
3
6
7
10
7
20
22
20
28
29
26
40
5
Jumlah klon
Klon Tangongon - Rendemen batang
0.3%
0 <0.93
<1.65 <2.38 Kisaran rendemen serat dari batang (%)
>2.38
60 Klon Tangongon - Rendemen pelepah
0.4%
0.5%
0.6%
0% 30 24
22
25
31
40
2
1
7
8
6 3
6
5
20 5
Jumlah klon
0.3%
0 <1.23
<2.18 <3.13 Kisaran rendemen serat dari pelepah (%)
>3.13
143
Lampiran 4. Sebaran nilai dan jumlah klon berdasarkan peubah panjang daun, lebar daun, dan rasio panjang/lebar daun tanaman di lapangan diantara populasi abaka klon Sangihe -1 varian yang diregenerasikan dari kalus embriogen dengan perlakuan berbagai konsentrasi EMS (0%, 0.3%, 0.4%, 0.5%, dan 0.6%), pada 16 bulan setelah tanam 60 Klon Sangihe-1 - Panjang daun 0.5%
0.6%
0%
1
1
2
16 16
22 12
18 12
17 10
20
25
40
10
Jumlah klon
0.3% 0.4%
0 <89.3
<135.9
<182.6
>182.6
Kisaran panjang daun (cm) 60 Klon Sangihe-1 - Lebar Daun 0.6%
0%
17
15
3
4
1
3
4
9
20
15 19
32
34
0.5%
40
7
Jumlah klon
0.3% 0.4%
0 <26.6
<39.1
<51.7
>51.7
Kisaran lebar daun (cm) 60 Klon Sangihe-1 - Rasio P/L daun 0.5%
0.6%
0%
0 <2.54
<3.28
<4.03
Kisaran rasio panjang/lebar daun
>4.03
1
2
4
2
2 1
6
10 8 8
13
20
16
23
25 27
40
8 6
Jumlah klon
0.3% 0.4%
144
Lampiran 5. Sebaran nilai dan jumlah klon berdasarkan peubah diameter batang, bobot batang, dan jumlah anakan tanaman di lapangan diantara populasi abaka klon Sangihe -1 varian yang diregenerasikan dari kalus embriogen dengan perlakuan berbagai konsentrasi EMS (0%, 0.3%, 0.4%, 0.5%, dan 0.6%), pada 16 bulan setelah tanam 60 0.5%
0.6%
0%
Klon Sangihe-1 - Diameter batang
1
1
12
12
20 19
22 11
9
20
26
40
12 18
Jumlah klon
0.3% 0.4%
<19.8
<30.4
<41.1
>41.1
Kisaran diameter batang (cm)
60 0.5%
0.6%
Klon Sangihe-1 - Bobot batang
0%
23
34 33
36
40 22 20
1 1
3
4
Jumlah klon
0.3% 0.4%
<3345
<6440
<9535
>9535
Kisaran bobot batang (g/tanaman)
60 0.5%
0.6%
Klon Sangihe-1 - Jumlah anakan
0%
21 24
13 9 10 8
0 <3.75
<6.25
<9.25
Kisaran jumlah anakan
>9.25
1
1
4
3 2 5
3
1
20
13
15 19
40
8
Jumlah klon
0.3% 0.4%
145
Lampiran 6. Sebaran nilai dan jumlah klon berdasarkan peubah bobot pelepah, panjang serat, rendemen serat dari batang dan rendemen serat dari pelepah tanaman di lapangan diantara populasi abaka klon Sangihe -1 varian yang diregenerasikan dari kalus embriogen dengan perlakuan berbagai konsentrasi EMS (0%, 0.3%, 0.4%, 0.5%, dan 0.6%), pada 16 bulan setelah tanam 60 Klon Sangihe-1 - Bobot pelepah
0.6%
0%
22
34
0.5% 34
36
40 21
1
2
5
20 2
Jumlah klon
0.3% 0.4%
0 <2493
<4895 <7298 Kisaran bobot pelepah (g/tanaman)
>7298
60
0.5%
0.6%
0%
13
12
17
3
4
2
1
8
6
8
12
13
20
18
40
6
Jumlah klon
Klon Sangihe-1 - Panjang serat
0.3% 0.4%
0 <93.7
<126.3
<159.0
>159.0
Kisaran panjang serat (cm) 60 Klon Sangihe-1 - Rendemen batang
0.5%
0.6%
0%
1
4
4
6
4
12
1
4
5
7
20
14
14
21
23
40
3
Jumlah klon
0.3% 0.4%
0 <0.93
<1.65
<2.38
>2.38
Kisaran rendemen serat dari batang (%) 60 Klon Sangihe-1 - Rendemen pelepah
0.5%
0.6%
0%
0 <1.23
<2.18
<3.13
Kisaran rendemen serat dari pelepah (%)
>3.13
1
1
1
3
4
5
11
22 12
14
1
2
3
8
20
17
15
40
2
Jumlah klon
0.3% 0.4%
