20. MEZIOBOROVÁ ČESKO-SLOVENSKÁ TOXIKOLOGICKÁ KONFERENCE TOXCON

1 | 20. Mezioborová česko-slovenská toxikologická konference 27.–29. 5. 2015 Brno

20. MEZIOBOROVÁ ČESKO-SLOVENSKÁ TOXIKOLOGICKÁ KONFERENCE

TOXCON 2015 20th Interdisciplinary Czech-Slovak Toxicology Conference TOXCON 2015

Hotel Myslivna, Brno, Czech Republic; May 27 – 29, 2015

2 | 20. Mezioborová česko-slovenská toxikologická konference 27.–29. 5. 2015 Brno

SEZNAM POSTERŮ LIST OF POSTERS Maximální rozměr posteru je 100 x 100 cm 1. STUDY OF BIOLOGICAL ACTIVITY OF HYDROXYNAPHTHALENE CARBOXANILIDE DERIVATIVES ON TUMOR CELL LINES Bábková Z.1, Polášková A.1,2, Adámik M.2, Helma R.1,2, Kos J.1, Goněc T.1, Jampílek J.1, Brázdová M.1,2 1 Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackého 1-3, Brno 2 Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Královopolská 135, Brno 2. CONTRIBUTIONS OF ARISTOLOCHIC ACID, OCHRATOXIN A, HEAVY METALS AND PHTHALATES ON AETIOLOGY OF BALKAN ENDEMIC NEPHROPATHY Bárta F.1, Levová K.1, Hodek P.1, Schmeiser H.H.2, Arlt V.M.3, Stiborová M.1 1 Department of Biochemistry, Faculty of Science, Charles University in Prague 2 Research Group Genetic Alterations in Carcinogenesis, German Cancer Research Center 3 Analytical and Environmental Sciences Division, MRC-PHE Centre for Environment and Health, King’s College London 3. EFFECTS OF CYANOBACTERIAL TOXINS ON LIVER PROGENITOR AND STEM CELLS Basu A., Čtveráčková L., Váňová S., Škovroňová R., Bláha L., Babica P. RECETOX - Research Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic 4. TRAMADOL - THE EFFECT ON DANIO RERIO IN THE GROWTH TEST Beránková P.1, Chmelová L.1, Zavadilová R.1, Svobodová Z.1 1 University of Veterinary and Pharmaceutical Sciences Brno, Palackého třída 1/3, Brno 5. EVALUATION OF CYTOTOXICITY INDUCED BY T-BHP Bezek Š., Mrvová N., Račková L., Ujházy E. Institute of Experimental Pharmacology and Toxicology Slovak Academy of Sciences, Dúbravská cesta 9, 841 04 Bratislava, Slovak Republic 6. IMMOBILIZED READY-TO-USE RECOMBINANT YEAST ASSAYS FOR DETECTION OF ESTROGENS AND ANDROGENS Bittner M., Jarque S., Hilscherová K. Masaryk University, Faculty of Science, RECETOX, Kamenice 5, CZ-62500 Brno, Czech Republic

3 | 20. Mezioborová česko-slovenská toxikologická konference 27.–29. 5. 2015 Brno

7. OXIDATIVE STRESS RESPONSES IN ZEBRAFISH (DANIO RERIO) AFTER SUBCHRONIC TONALID EXPOSURE Blahová J.1, Divišová L.1, Enevová V.1, Plhalová L.1, Doubková V.1, Maršálek P.1, Svobodová Z.1 1 University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Department of Veterinary Public Health and Animal Welfare, Palackého tř. 1/3, Brno, Czech Republic 8. ASSESSMENT OF SPECIFIC OXIDATIVE STRESS BIOMARKERS IN TISSUES AFTER THE INHALATION EXPOSURE OF MICE TO CDO NANOPARTICLES Bláhová L.1, Podborská M. 1, Kohoutek J.1, Lebedová J.1, Bláha L.1, Večeřa Z.2, Buchtová M.3, Míšek I.3, Hilscherová K.1 1 Masaryk University, RECETOX, Kamenice 753/5, CZ62500 Brno, Czech Republic 2 Institute of Analytical Chemistry v. v. i., Academy of Sciences, Brno, Czech Republic 3 Institute of Animal Physiology and Genetics v. v. i., Academy of Sciences, Brno, Czech Republic 9. A STUDY ON 17ALPHA-ETHINYLESTRADIOL METABOLISM IN RAT AND PLEUROTUS OSTREATUS Bořek-Dohalská L.1, Valášková P.1, Kubíčková B.1, Svobodová K.2, Cajthaml T.2, Stiborová M.1 1 Department of Biochemistry, Faculty of Science, Charles University in Prague, Albertov 6, 128 43 Prague 2, Czech Republic 2 Institute of Microbiology, Academy of Science of the Czech Republic, Vídeňská 1083, 142 20 Prague 4, Czech Republic 10. ROLE OF DIHYDROMYRICETIN IN CYTOCHROME P450 MEDIATED METABOLISM Boštíková Z.1, Pávek P. 2, Stiborová M.1, Hodek P.1 1 Department of Biochemistry, Faculty of Science, Charles University in Prague, Hlavova 8, 128 43 Prague 2 2 Department of Pharmacology and Toxicology, Faculty of Pharmacy, Charles University in Prague, Heyrovského 1203, 500 05 Hradec Králové 11. NEONATAL WITHDRAWAL SYNDROME AND PERINATAL ASPHYXIA. HOW TO MANAGE THE NEWBORN? Brucknerová I.1, Mach M.2, Dubovický M.2, Ujházy E.2 1 Neonatal Department of Intensive Medicine, Medical Faculty, Comenius University in Bratislava and Children´s Hospital in Bratislava, Slovak Republic 2 Institute of Experimental Pharmacology & Toxicology, Slovak Academy of Sciences, Bratislava, Slovak Republic 12. FERROUS AND FERRIC STATES OF CYTOCHROMES P450 IN INTACT ESCHERICHIA COLI CELLS - POSSIBLE ROLE OF CYTOCHROME P450-FLAVODOXIN INTERACTIONS Culka M., Milichovský J., Jeřábek P., Stiborová M., Martínek V. Department of Biochemistry, Faculty of Science, Charles University in Prague, Albertov 6, 128 43 Prague 2, Czech Republic

4 | 20. Mezioborová česko-slovenská toxikologická konference 27.–29. 5. 2015 Brno

13. NANOCARRIERS WITH ENCAPSULED DOXORUBICIN AND THEIR EFFECT ON CANCER CELL LINES Černá T.1, Hraběta J.2, Groh T.1, Dostálová S.3, Nguyen H.V.3, Moulick A.3, Kizek R.3, Adam V.3, Eckschlager T.2, Stiborová M.1 1 Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic 2 Department of Pediatric Hematology and Oncology, 2nd Medical Faculty, Charles University and University Hospital Motol, Prague, Czech Republic 3 Laboratory metallomics and nanotechnology, Mendel University in Brno and Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic European Union 14. MODULATION OF CHEMICALLY- AND ONCOGENE-INDUCED INHIBITION OF GAP JUNCTIONAL INTERCELLULAR COMMUNICATION BY CHEMOPREVENTIVE AGENTS Čtveráčková L.1, Trosko J. E. 2, Upham B. L. 2, Babica P.1, 3 1 RECETOX - Research Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic 2 Department of Pediatrics and Human Development, Michigan State University, 1129 Farm Ln, East Lansing, MI 48824, USA 3 Department of Experimental Phycology and Ecotoxicology, Institute of Botany ASCR, Lidická 25/27, 602 00 Brno, Czech Republic 15. DETERMINATION OF TOXICITY OF COPPER (II) SCHIFF BASE COMPLEXES DERIVED FROM AMINO ACIDS Dlouhá M. 1, Keltošová S. 1, Račanská E. 1 1 Department of Human Pharmacology and Toxicology, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackého 1-3, Brno 16. EFFECT OF BETA-GLUCAN ON OXIDATIVE STRESS PARAMETERS IN COMMON CARP (CYPRINUS CARPIO) EXPOSED TO OXYTETRACYCLINE ANTIBIOTICS Dobšíková R., Blahová J., Charvátová N., Kobylková L., Svobodová Z. University of Veterinary and Pharmaceutical Sciences Brno, Department of Veterinary Public Health and Animal Welfare, Palackého tř. 1/3, 612 42 Brno, Czech Republic 17. DETECTION OF ANIMAL’S POISONING OF ANTICOAGULANT RODENTICIDES Doubková V.1, Maršálek P.1, Modrá H.1 1 Department of Veterinary Public Health and Toxicology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic, 612 42, Brno, Czech Republic

5 | 20. Mezioborová česko-slovenská toxikologická konference 27.–29. 5. 2015 Brno 18. THE INFLUENCE OF COMBINED APPLICATION OF SUDAN I AND BENZO(A)PYRENE ON GENE EXPRESSION OF BIOTRANSFORMATION ENZYMES AND BENZO(A)PYRENE GENOTOXICITY IN RATS Dračínská H.1, Moserová M.1, Indra R.1, Bárta F.1, Gajdík Z.1, Schmeiser H. H.2, Frei E.2, Ulrich C. M.2, Arlt V. M.3, Hodek P.1, Stiborová M.1 1 Department of Biochemistry, Faculty of Science, Charles University in Prague, Hlavova 8, 12843 Prague 2, Czech Republic; 2German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, 69120 Heidelberg, Germany; 3MRC-PHE Centre for Environmental & Health, King's College London, 150 Stamford Street, London SE1 9NH, UK. 19. IN VIVO VIABILITY OF CHEMICALLY MODIFIED ERYTHROCYTES AFTER TRANSFUSION IN RATS Dušková Š.1, Mráz J.1, Linhart I.2, Hanzlíková I.1, Dabrowská L.1, Chrástecká H.1, Vajtrová R.1 and Alánová P.3 1 Centre of Occupational Health, National Institute of Public Health, Prague 2 Department of Organic Chemistry, University of Chemistry and Technology, Prague 3Institute of Physiology, Academy of Sciences of the Czech Republic, Prague 20. CONCENTRATION OF HEAVY AND TOXIC METALS IN FISH AND SEDIMENTS FROM THE BASIN MORAVA, CZECH REPUBLIC Dvořák P.1, Andreji J.2, Dvořáková – Líšková Z.3 1 Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Czech Republic 2 Department of Poultry Science and Small Animal Husbandry, Slovak University of Agriculture, Nitra, Slovak Republic 3 Faculty of Economy, University of South Bohemia, Ceske Budejovice, Czech Republic 21. AUTOMATIC IMAGE ANALYSIS FOR EVALUATION OF CHEMICAL EFFECTS IN 3D CELL CULTURE MODELS Dydowiczová A., Basu A., Sovadinová I., Babica P. RECETOX - Research Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic 22. USING GASTROPODS IN ECOTOXICITY ASSESSEMENT OF REAL CONTAMINATED MATRIXES Fojt P.1 , DeVaufleury A.2 1 Faculty of Chemistry, University of Technology, Purkyňova 118, Brno 2 Department of Chrono-Environment, University of Franche-Comté, Place Leclerc, Besancon 23. EFFECT OF ANTIPSYCHOTIC OLANZAPINE ON ISCHEMIC-REPERFUSION INDUCED MYOCARDIAL INJURY Gulač P.1, Vicen M.1, Kráľová E.1, Tomášová L1., Knezl V.2, Stankovičová T.1 1 Comenius University Bratislava, Faculty of Pharmacy, Department of Pharmacology and Toxicology 2 Institute of Experimental Pharmacology & Toxicology, Slovak academy of Sciences, Bratislava

6 | 20. Mezioborová česko-slovenská toxikologická konference 27.–29. 5. 2015 Brno 24. BIOLOGICAL FATE OF STYRENE OXIDE ADDUCTS WITH GLOBIN: IDENTIFICATION OF DEGRADATION PRODUCTS IN RAT URINE Hanzlíková I. 1, Mráz J.1, Linhart I.2, Dušková Š.1, Dabrowská L.1, Moulisová A.2, Hejl K.2, Chrástecká H.1 and Vajtrová R.1 1 Centre of Occupational Health, National Institute of Public Health, Prague 2 Department of Organic Chemistry, University of Chemistry and Technology, Prague 25. COMPARATIVE STUDY OF THE ECOTOXICOLOGICAL EFFECT OF CD IN FOLSOMIA CANDIDA, ENCHYTRAEUS CRYPTICUS AND LACTUCA SATIVA Havelková B., Beklová M. Department of Ecology and Diseases of Game, Fish and Bees, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1/3, CZ612 42 Brno, Czech Republic 26. BINDING OF MUTANT P53 TO G-RICH REGIONS OF TARGET GENES AND REGULATION OF THEIR TRANSCRIPTION Helma R.1,2, Quante T.3, Kejnovská I.1, Polášková A.1, Navrátilová L.1, Petr M.1, Adámik M.1, Bábková Z.2, Holaňová L.2, Krejčí A.1, Deppert W.3, Tolstonog G.V.2, Brázdová M.1,2 1 Institute of Biophysics, AS CR, v.v.i, Královopolská 135, Brno, 2 University of Veterinary and Pharmaceutical Sciences Brno, Palackého 1-3, Brno 3 Heinrich-Pette Institute; Martini strasse 52, Hamburg, Germany 27. TOXICITY OF NORFLOXACIN FOR COMMON CARP (CYPRINUS CARPIO L.) FOCUSED ON SOMATIC PARAMETERS Charvátová N.1, Dobšíková R.1, Blahová J.1, Plhalová L.1, Sehonová P.1, Živná D.1, Želinská G. 1, Prokeš M.2, Svobodová Z.1 1 University of Veterinary and Pharmaceutical Sciences Brno, Department of Veterinary Public Health and Animal Welfare, Palackého tř. 1/3, 612 42 Brno, Czech Republic 2 Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, v.v.i., Brno, Czech Republic 28. INVESTIGATION ON HISTOPATHOLOGICAL ALTERATIONS OF SUB-ACUTE PERACETIC ACID EXPOSURE TO SIGNAL CRAYFISH (PACIFASTACUS LENIUSCULUS) Chupani L., Zusková E., Kouba A., Stará A., Velíšek J. University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, CR 29. CONTRIBUTIONS OF INDIVIDUAL CYTOCHROMES P450 TO METABOLISM OF BENZO(A)PYRENE IN HUMAN AND RAT LIVERS Indra R. 1, Moserová M. 1, Hodek P. 1, Bořek-Dohalská L.1, Schmeiser H.H.2, Frei E.2, Arlt V.M. 3 , Stiborová M. 1 1 Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic; 2 German Cancer Research Center (DKFZ), Heidelberg, Germany; 3MRC-PHE Centre for Environmental & Health, King's College London, London SE1 9NH, UK

7 | 20. Mezioborová česko-slovenská toxikologická konference 27.–29. 5. 2015 Brno 30. RESIDUES OF SELECTED ANTIBIOTICS IN THE SOUTH MORAVIAN RIVERS, CZECH REPUBLIC Járová K.1, Vávrová M.2, Kolečkářová A.1 1 University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Department of Ecology & Game, Fish and Bees Diseases, Palackého tř. 1/3, 612 42 Brno, Czech Republic, [email protected] 2 Brno University of Technology, Faculty of Chemistry, Institute of Chemistry and Technology of Environmental Protection, Purkyňova 118, 612 00 Brno, Czech Republic 31. RETINOID-LIKE COMPOUNDS PRODUCED BY WATER BLOOMS OF WIDESPREAD CYANOBACTERIUM MICROCYSTIS AERUGINOSA Javůrek J., Sychrová E., Smutná M., Bittner M., Kohoutek J., Adamovský O., Nováková K., Smetanová S., Hilscherová K. RECETOX – Research Centre for Toxic Compounds in the Environment, Masaryk University, Faculty of Science, Brno, Czech Republic 32. EPIGENOME AND DIFFERENTIATION AFFECTING DRUGS CHANGE EXPRESSION OF NUCLEOSIDE TRANSPORTERS IN PLACENTAL BEWO AND JEG-3 CELL LINES Jirásková L., Červený L., Štaud F. Department of Pharmacology and Toxicology, Charles University in Praque, Faculty of Pharmacology in Hradec Králové 33. SYSTEMIC OXIDATIVE STRESS IN STREPTOZOTOCIN – INDUCED DIABETES IN RATS – EFFECT OF ROSMARINIC ACID Kaprinay B., Sotníková R., Navarová J. Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava, Slovakia 34. NUCLEOSIDE TRANSPORTERS DOES NOT AFFECT TRANSPORT OF ZIDOVUDINE ACROSS THE PLACENTA Karbanová S., Neumanová Z., Červený L., Štaud F. Department of pharmacology and toxicology, Faculty of Pharmacy in Hradec Králové, Charles University in Prague, Czech Republic 35. A COMPARISON OF THE REACTIVATING AND THERAPEUTIC EFFICACY OF TWO NOVEL BISPYRIDINIUM OXIMES (K727, K733) WITH THE OXIME HI-6 AND OBIDOXIME IN SARINPOISONED RATS AND MICE Kassa J., Šepsová V., Matoušková L., Horová A., Musílek K. Department of Toxicology and Military Pharmacy, Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic 36. THE OCCURENCE OF MERCURY IN ONE OF THE PART IN EAST SLOVAKIA Kimáková T. Department of Public Health and Hygiene, Faculty of Medicine, University of P. J. Šafárik in Košice

8 | 20. Mezioborová česko-slovenská toxikologická konference 27.–29. 5. 2015 Brno 37. WORKERS AND THEIR EXPOSURE TO PCB Kimáková T. 1, Šak M. 2, Nevolná Z. 3, Farkašová Iannacone S. 4 1 Department of Public Health and Hygiene, Faculty of Medicine, University of P. J. Šafárik in Košice 2 Department of Dermatovenerology, Faculty of Medicine, University of P. J. Šafárik in Košice 3 St. Elizabeth University of Health and Social Work in Bratislava 4 Department of Forensic Medicine, Faculty of Medicine, University of P. J. Šafárik in Košice 38. USE OF ISOPROTERENOL-INDUCED MODEL OF CARDIOTOXICITY AND CTNI DURING EVALUATION OF CARDIOPROTECTIVE EFFECT OF 44BU AND CARVEDILOL IN RATS Kolmanová E.1, Bartošová L.1, Mokrý P.2, Parák T.1, Suchý P.1 1 Department of Human Pharmacology and Toxicology, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1-3, 612 42 Brno, Czech Republic 2 Department of Chemical Drugs Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1-3, 612 42 Brno, Czech Republic 39. PROTECTIVE EFFECT OF NARINGENIN IN ARSENIC-INDUCED OXIDATIVE DAMAGE IN RATS Kotyzová D., Bludovská M., Hradecký O., Eybl V. Department of Pharmacology & Toxicology, Faculty of Medicine, Charles University, Pilsen 40. EVALUATION OF MERCURY CONTAMINATION IN DOGS USING HAIR ANALYSIS Král T.1, Blahová J.1, Sedláčková L.1, Svobodová Z.1 1 Department of Veterinary Public Health and Animal Welfare University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic 41. DOES THE ANIMAL TREATMENT WITH NEURAMINIDASE INDUCE AIRWAY CONDITIONS SIMILAR TO THOSE OF LUNGS FROM CF PATIENTS? Kubíčková B.1, Hadrabová J.1, Mandys V.2, Vadinská K.2, Stiborová M.1, Hodek P.1 1 Department of Biochemistry, Faculty of Science, Charles University in Prague, Albertov 6, 128 43 Prague 2, Czech Republic 2 Department of Pathology, Third Faculty of Medicine, Charles University, Ruská 87, 10000 Prague 10, Czech Republic 42. PRAVASTATIN IS EFFECTIVELY ELIMINATED BY RENAL TUBULAR SECRETION IN RATS WITH OBSTRUCTIVE CHOLESTASIS Laho T.1, Kadová Z.1, Doleželová E.1,2, Cermanová J.1, Nováková L.3, Vlčková H.3, Mičuda S.1 1 Department of Pharmacology, Charles University in Prague, Faculty of Medicine in Hradec Kralove; 2Department of Biological Sciences, 3Department of Analytical Chemistry, Charles University in Prague, Faculty of Pharmacy in Hradec Kralove; Czech Republic. 43. INTERDISCIPLINARY TOXICOLOGY – SEVEN YEARS IN SCIENTIFIC COMMUNITY Mach M., Ujházy E., Dubovický M. Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Dubravska cesta 9, 841 04 Bratislava, Slovakia. e-mail: [email protected]

9 | 20. Mezioborová česko-slovenská toxikologická konference 27.–29. 5. 2015 Brno 44. DEVELOPMENT AND VALIDATION OF A FAST LC-MS/MS METHOD FOR QUANTIFICATION OF URAPIDIL IN HUMAN SERUM Marešová V.1, Oktábec Z.1, Čabala R.1, Kopecká A.1, Štrauch B.2, Petrák O.2, Holaj R.2, Zelinka T.2, Widimský J. Jr 2 1 Department of Toxicology, Institute of Forensic Medicine and Toxicology, 1 st Faculty of Medicine, Charles University in Prague and General University Hospital in Prague 2 rd 3 Department of Internal Medicine, 1st Faculty of Medicine, Charles University in Prague and General University Hospital in Prague 45. METABOLIZATION OF PRAZIQUANTEL AND FENBENDAZOLE IN RAINBOW TROUT AFTER ORAL ADMINISTRATION Marková-Soukupová Z.1, Doubková V.2, Maršálek P.2, Svobodová Z.2, Palíková M.1 1 Department of Ecology and Diseases of Game, Fish and Bees, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého třída 1/3, Brno 2 Department of Veterinary Public Health and Animal, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého třída 1/3, Brno 46. EFFECT OF THE T-2 TOXIN ON THE IMMUNE RESPONSES OF RAINBOW TROUT ONCORHYNCHUS MYKISS Matejová I. 1, Palíková M. 2, Modrá H. 1, Hyršl P. 3, Vojtek L. 3, Svobodová Z. 1 1 Department of Veterinary Public Health and Welfare, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1/3, Brno 2 Department of Ecology and Diseases of Game, Fish and Bees, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1/3, Brno 3 Institute of Experimental Biology, Faculty of Science, Masaryk University, Kotlarska 2, Brno 47. EFFECT OF CHRONIC MILD STRESS AND/OR THE ANTIDEPRESSANT VENLAFAXINE TREATMENT ON MATERNAL BEHAVIOR IN RATS Melicherčíková, K.1,2, Császár, E.1,2, Ujházy, E.1, Dubovický, M.1 1 Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Bratislava, Slovak Republic 2 Department of Pharmacology, Jessenius Faculty of Medicine, Comenius University, Martin, Slovak Republic 48. ANTIPROLIFERATIVE AND IMMUNOMODULATORY ACTIVITY OF NEW FLUOROQUINOLONE ON DIFFERENT CELL LINES Melušová M.1, Paulovičová E.2, Paulovičová L.2, Janošková M.1, Milata V.3, Jantová S.1 1 Institute of Biochemistry, Nutrition and Health Protection, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, Bratislava, Slovakia 2 Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, Bratislava, Slovakia 3 Institute of Organic Chemistry, Catalysis and Petrochemistry, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, Bratislava, Slovakia

10 | 20. Mezioborová česko-slovenská toxikologická konference 27.–29. 5. 2015 Brno 49. TOXICITY OF SELECTED INSECTICIDES AND HEAVY METALS IN MOUSE-EARED BAT CELL LINES Mikšíková M1., Banďouchová H. 1, Linhart P1. Brichta J. 1, Hrubá H. 1, Kováčová V. 1, Zukal J.2,3, Köllner B.4, Pikula J. 1 1 Department of Ecology and Diseases of Game, Fish and Bees, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého 1/3, 612 42 Brno, Czech Republic 2 Department of Botany and Zoology, Masaryk University, Kotlářská 2, 61137 Brno, Czech Republic 3 Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, Květná 8, 603 65 Brno, Czech Republic 4 Friedrich-Loeffler-Institut (FLI), Südufer 10, 17493 Greifswald – Insel Riems, Germany 50. COMPARISON OF THE ADSORPTION CAPACITY AND TRAPPING EFFECTIVENESS OF DIOSMECTITE AND CHARCOAL ON THE COMPOUNDS CAUSING THE MOST FREQUENT INTOXICATIONS IN ACUTE MEDICINE Mináriková M.1, Vyskočilová E.2, Sedláček J.3, Stiborová M.1, Martínková M.1 1 Department of Biochemistry, Faculty of Science, Charles University in Prague, Hlavova (Albertov) 2030-8, Prague 2, Czech Republic 2 Institute of Chemical Technology Prague, Faculty of Chemical Technology, Department of Organic Technology, Technická 1905, 166 28 Prague 6, Czech Republic 3 Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University in Prague, Hlavova (Albertov) 2030-8, Prague 2, Czech Republic 51. EFFECTS OF PHARMACEUTICALS IN TWO ALTERNATIVE AQUATIC TOXICITY BIOASSAYS WITH D. RERIO EMBRYOS AND RT GILL W1 FISH CELLS IN VITRO Mlnaříková M.1., Pípal M. 1, Jonáš A.1, Bečanová, J.1, Bláha, L.1 1 Masaryk University, RECETOX, Kamenice 753/5, CZ62500 Brno, Czech Republic 52. EFFECTS OF COMBINATIONS OF SELECTED ENDOCRINE DISRUPTORS AND VITAMINS ON STEROID HORMONE PRODUCTION BY PORCINE OVARIAN GRANULOSA CELLS Mlynarčíková A., Ficková M., Scsuková S. Institute of Experimental Endocrinology SAS, Vlárska 3, 83306 Bratislava, Slovakia 53. A STUDY ON EFFICIENCIES OF ELLIPTICINE TO MODULATE EXPRESSION OF CYTOCHROMES P450 AS A POTENTIAL LIGAND OF ARYL HYDROCARBON-, GLUCOCORTICOID- OR PREGNANE X- RECEPTORS Moserová M.1, Mrízová I. 1, Švécarová M. 2, Dvořák Z. 2, Stiborová M.1 1 Department of Biochemistry, Faculty of Science, Charles University in Prague, Albertov 6, 128 43 Prague 2, Czech Republic 2 Department of Cell Biology and Genetics, Faculty of Science, Palacky University, Šlechtitelu 11, 783 71 Olomouc, Czech Republic

11 | 20. Mezioborová česko-slovenská toxikologická konference 27.–29. 5. 2015 Brno 54. MATERNAL SMOKING IN PREGNANCY Nevolná Z. 1 , Kimáková T. 2 1 St. Elizabeth University of Health and Social Work in Bratislava 2 Department of Public Health and Hygiene, Faculty of Medicine, University of P. J. Šafárik in Košice 55. EFFECT OF OXALIC ACID APPLIED BY SUBLIMATION ON HONEY BEE COLONY FITNESS – A COMPARISON WITH AMITRAZ Papežíková I., Heumannová R., Fronc M., Palíková M. Department of Ecology and Diseases of Game, Fish and Bees, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic 56. THE EFFECTS OF SUBCHRONIC EXPOSURE TO NEEMAZAL T/S ON ZEBRAFISH (DANIO RERIO) Plhalová L.1, Blahová J.1, Divišová L.1, Enevová V.1, Casuscelli di Tocco F.2, Faggio F.2, Tichý F.3, Svobodová Z.1 1 Department of Veterinary Public Health and Animal Welfare, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1/3, 612 42 Brno, Czech Republic 2 Department of Biological and Environmental Sciences, University of Messina, Viale Ferdinando Stagno d'Alcontres 31, 98166, S. Agata, Messina, Italy 3 Department of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1/3, 612 42 Brno, Czech Republic 57. SARIN HAS IMPACT ON OXIDATIVE HOMEOSTASIS AND STANDARD BIOCHEMICAL BLOOD MARKERS Pohanka M. 1, Románek J. 1, Fusek J. 1, Pikula J. 2 1 Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 50001 Hradec Kralove, Czech Republic 2 Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1/3, 612 42 Brno, Czech Republic 58. INFLUENCE OF P53 CONFORMATION ON RESPONSE TO CYTOTOXIC TREATMENT IN GLIOBLASTOMA AND COLON CELL LINES Polášková A.1,2, Adámik M.2, Bábková Z.1, Helma R.1,2 and Brázdová M.1,2 1 Faculty of Pharmacy, University of Veterinary and Pharmaceutic Sciences Brno, Palackého 1-3, Brno 2 Institute of Biophysics, Academy of Sciences of the CR, v.v.i., Královopolská 135, Brno

12 | 20. Mezioborová česko-slovenská toxikologická konference 27.–29. 5. 2015 Brno 59. EVALUATION OF CARNOSINE, TROLOX AND NOVEL TROLOX-CARNOSINE EFFECT IN THE TREATMENT OF ADJUVANT ARTHRITIS WITH IMPACT ON OXIDATIVE STRESS IN THE BRAIN Poništ S.1, Slovák L.1, Kuncírová V.1, Kucharská J.2, Fedorova T.3, Logvinenko A.3, Muzychuk O.3, Mihalová D.1 and Bauerová K.1 1 Institute of Experimental Pharmacology and Toxicology of Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava, Slovak Republic, [email protected] 2 Faculty of Medicine, Comenius University, Sasinkova 4, Bratislava, Slovak Republic 3 Research Center of Neurology, Moscow, Volokolamskoye shosse 80, 125367 Moscow, Russian Federation 60. COMPARISON OF THE EFFECT OF FOUR ANAESTHETICS ON HAEMATOLOGICAL PROFILE, OXIDATIVE STRESS AND ANTIOXIDANT ENZYMES IN BARBEL (BARBUS BARBUS) Příborský J., Stará A., Řežábek J., Zusková E., Lepič P., Velíšek J. University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Czech Republic 61. EFFECT OF POLYMERIC NANOPARTICLE POLY (ETHYLENE GLYCOL)-BLOCK-POLY (LACTIC ACID) (PEG-B-PLA) ON IN VITRO LH RELEASE FROM ANTERIOR PITUITARY CELLS IN FEMALE RATS Scsuková S.1, Mlynarčíková A.1, Kiss A.1, Rollerová E.2 1 Institute of Experimental Endocrinology, Slovak Academy of Sciences, Vlárska 3, Bratislava, Slovakia 2 Slovak Medical University, Department of Toxicology, Limbová 12, Bratislava, Slovakia 62. RETINOID-LIKE COMPOUNDS PRODUCED BY PHYTOPLANKTON AND THEIR EFFECTS ON THE EMBRYONIC DEVELOPMENT OF XENOPUS LAEVIS Smutná M., Večerková J., Nekvapilová A., Hilscherová K. RECETOX – Research Centre for Toxic Compounds in the Environment, Masaryk University, Faculty of Science, Brno, Czech Republic 63. EFFECTS OF ACUTE EXPOSURE TO DELTAMETHRIN ON BIOMARKER OF OXIDATIVE STRESS AND ANTIOXIDANT RESPONSE ON COMMON CARP (CYPRINUS CARPIO) Stará A., Máchová J., Příborský J., Velíšek J. Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Czech Republic 64. TIME-DEPENDENT TRIMETHYLTIN EFFECT ON SYNAPTIC TRANSMISSION IN THE RAT HIPPOCAMPUS Stará V., Ujházy E., Gáspárová Z. Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Bratislava, Slovakia

13 | 20. Mezioborová česko-slovenská toxikologická konference 27.–29. 5. 2015 Brno 65. POISONINGS OF FARM ANIMALS CAUSED BY TOXIC PLANTS Široká Z.1, Svobodová, Z.1, Pána O.2, Kučerová Chrpová V.3 1 University of Veterinary and Pharmaceutical Sciences Brno, Department of Veterinary Public Health and Animal Welfare, Palackého tř. 1/3, 612 42 Brno, Czech Republic 2 Veterinary Clinic, Na Piketě 542/III, 37701 Jindřichův Hradec, Czech Republic 3 Czech University of Life Sciences Prague, Department of Veterinary Sciences, Kamýcká 129, 165 21 Praha 6, Czech Republic 66. EFFECTS OF SALINITY ON TERRESTRIAL ISOPODS PORCELLIO SCABER Škarková P.a, Vávrová M.a, Jemec A. b, Kos M.b, Drobne D.b,c,d a Brno University of Technology, Faculty of Chemistry, Purkyňova 118, Brno, Czech Republic b University of Ljubljana, Biotechnical Faculty, Department of Biology, Večna pot 111, 1000 Ljubljana, Slovenia c Centre of Excellence in Advanced Materials and Technologies for the Future (CO NAMASTE), Jozef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia d Centre of Excellence in Nanoscience and Nanotechnology (CO Nanocenter), Jozef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia 67. A STUDY OF INTERACTIONS OF CYCLIN-DEPENDENT KINASE INHIBITORS AZD5438 AND R547 WITH ABC DRUG EFFLUX TRANSPORTERS ABCB1, ABCG2 AND ABCC1 Šorf A.1, Číhalová D.1, Čečková M.1, Štaud F.1 1 Department of Pharmacology and Toxicology, Charles University in Prague, Faculty of Pharmacy, Heyrovského 1203, Hradec Králové 68. THE IMPACT OF ALCOHOL ON MORTALITY IN THE REGION OF NORTHERN SLOVAKIA Tumová I.1, Račanská E.1, Berzáková M.1, Straka Ľ.2 1 Faculty of Pharmacy, Comenius University, Odbojárov 10, Bratislava, Slovakia 2 Jessenius Faculty of Medicine Comenius University Martin, Slovakia 69. THE EFFECT OF DIFFERENT N-DONOR LIGANDS ON THE ELECTRONIC STRUCTURE, MAGNETIC PROPERTIES AND BIOLOGICAL ACTIVITIY OF IRON(II/III)-SALOPHEN COMPLEXES Vančo J. 1, Šindelář Z. 1, Trávníček Z. 1, Dvořák Z. 2 1 Regional Centre of Advanced Technologies and Materials & Department of Inorganic Chemistry, Faculty of Science, Palacký University in Olomouc, 17.listopadu 12, CZ-771 46 Olomouc, Czech Republic 2 Department of Cell Biology and Genetics, Faculty of Science, Palacký University in Olomouc, Šlechtitelů 27, CZ-783 71 Olomouc, Czech Republic 70. EFFECTS OF TERBUTHYLAZINE ON EARLY LIFE STAGES OF COMMON CARP (CYPRINUS CARPIO L.) Velíšek J., Stará A., Koutnik D., Zusková E. University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Czech Republic

14 | 20. Mezioborová česko-slovenská toxikologická konference 27.–29. 5. 2015 Brno 71. PREPARATION AND EVALUATION OF CARRIERS FOR DETECTION OF CHOLINESTERASE INHIBITORS Vetchý D.1, Pitschmann V.2, Vetchá M.3, Kašparovský T.4, Matějovský L.5 1 Department of Pharmaceutics, Faculty of Pharmacy, Veterinary and Pharmaceutical University Palackého tř. 1, Brno 2 Oritest Ltd., Staropramenná 17, Praha 3 Pharmacy, Mendlovo nám. 14, Brno 4 Department of Biochemistry, Faculty of Science, Masaryk University, Kotlářská 2, Brno 5 Faculty of Environmental Technology, University of Chemistry and Technology, Technická 5, Praha 72. SILYMARIN FLAVONOLIGNANS DO NOT AFFECT THE EXPRESSION OF HEME OXYGENASE-1 IN RAW264.7 AND HEPG2 CELLS Vrba J.1,2, Roubalová L.1,2, Křen V.3, Ulrichová J.1,2 1 Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 3, Olomouc 77515, Czech Republic 2 Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 3, Olomouc 77515, Czech Republic 3 Institute of Microbiology, Center for Biocatalysis and Biotransformation, Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague 14220, Czech Republic 73. EFFECTS OF ENVIRONMENTAL CONTAMINANTS ON GAP JUNCTIONAL INTERCELLULAR COMMUNICATION IN SOMATIC TESTICULAR CELLS Yawer A., Macíková P., Babica P., Sovadinová I. RECETOX - Research Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic 74. ASSESSMENT OF FORMALDEHYDE RESINS ECOTOXICITY IN RESPECT TO AQUATIC ECOSYSTEM Zlámalová Gargošová H.1, Kalčíková G.1, Vávrová M.1 1 Institute of Chemistry and Technology of Environmental Protection, Faculty of Chemistry, Brno University of Technology Brno, Czech Republic 75. EXPERIMENTAL TREATMENT OF POMPHORHYNCHUS LAEVIS (ACANTHOCEPHALA) INFECTION IN BARBEL (BARBUS BARBUS) Zusková E., Piačková V., Kolářová J., Máchová J., Chupani L., Steinbach Ch., Velíšek J. University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, CR