Lampiran 1. Hasil analisis kandungan asam lemak dari minyak alpukat
49 Universitas Sumatera Utara
Lampiran 2. Hasil analisis kandungan vitamin E dari minyak alpukat
50 Universitas Sumatera Utara
Lampiran 3. Bagan alir proses pembuatan dasar krim
Fase minyak
Fase air
- asam stearat
- sorbitol
- setil alkohol
- propilen glikol
- dimasukkan ke dalam cawan penguap
- trietanolamin - metil paraben
- dilebur di atas penangas air pada suhu 70°C - dilarutkan dalam air panas yg telah ditakar pada suhu 70°C Fase minyak + fase air - digerus konstan di dalam lumpang panas - ditambahkan 3 tetes pengaroma Dasar krim
51 Universitas Sumatera Utara
Lampiran 4. Bagan alir proses pembuatan sediaan krim anti-aging
Fase minyak
Fase air
- asam stearat
- sorbitol
- setil alkohol
- propilen glikol
- dimasukkan ke dalam cawan penguap
- trietanolamin - metil paraben
- dilebur di atas penangas air pada suhu 70°C - dilarutkan dalam air panas yg telah ditakar pada suhu 70°C
Fase minyak + Fase air - digerus konstan di dalam lumpang panas - ditambahkan minyak alpukat - ditambahkan 3 tetes pewangi Krim anti-aging
52 Universitas Sumatera Utara
Lampiran 5. Gambar alat
A
B
C
Keterangan : A: Moisture Checker (Aramo-SG), B: Skin Analyzer (Aramo-SG), C: pH meter (Hanna Instrument)
53 Universitas Sumatera Utara
Lampiran 6. Sampel minyak alpukat (avocado oil) “Green Tosca”
54 Universitas Sumatera Utara
Lampiran 7. Gambar sediaan
-setelah selesai dibuat
Krim A
Krim B
Krim C
B
Krim D D
Krim E
-setelah penyimpanan 12 minggu
Krim B
Krim C
Krim A
Krim D
Krim E
Keterangan: Krim A: blanko, Krim B: krim minyak alpukat 5%, Krim C: krim minyak alpukat 10%, Krim D: krim minyak alpukat 15%, Krim E: krim minyak alpukat 20%.
55 Universitas Sumatera Utara
Lampiran 8. Gambar hasil uji evaluasi sediaan krim
Krim A
Krim B
Krim C
Krim D
Krim E
Gambar uji homogenitas sediaan
Krim A
Krim B
Krim C
Krim D
Krim E
Gambar uji penentuan tipe emulsi Keterangan: Krim A: blanko, Krim B: krim minyak alpukat 5%, Krim C: krim minyak alpukat 10%, Krim D: krim minyak alpukat 15%, Krim E: krim minyak alpukat 20%.
56 Universitas Sumatera Utara
Lampiran 9. Contoh hasil pengukuran skin analyzer
Kadar air (moisture) Kondisi awal :
Pemulihan minggu I :
Pemulihan minggu II :
Pemulihan minggu III :
Pemulihan minggu IV :
57 Universitas Sumatera Utara
Lampiran 9. (Lanjutan) Kehalusan (evenness) Kondisi awal :
Pemulihan minggu I :
Pemulihan minggu II :
58 Universitas Sumatera Utara
Lampiran 9. (Lanjutan) Pemulihan minggu III :
Pemulihan minggu IV :
59 Universitas Sumatera Utara
Lampiran 9. (Lanjutan) Noda (spot) Kondisi awal :
Pemulihan minggu I :
60 Universitas Sumatera Utara
Lampiran 9. (Lanjutan) Pemulihan minggu II :
Pemulihan minggu III :
61 Universitas Sumatera Utara
Lampiran 9. (Lanjutan) Pemulihan minggu IV :
62 Universitas Sumatera Utara
Lampiran 9. (Lanjutan) Keriput (wrinkle) Kondisi awal :
Pemulihan minggu I :
63 Universitas Sumatera Utara
Lampiran 9. (Lanjutan) Pemulihan minggu II :
Pemulihan minggu III :
64 Universitas Sumatera Utara
Lampiran 9. (Lanjutan) Pemulihan minggu IV :
65 Universitas Sumatera Utara
Lampiran 10. Contoh surat pernyataan sukarelawan SURAT PERNYATAAN PERSETUJUAN IKUT SERTA DALAM PENELITIAN Saya yang bertandatangan dibawah ini : Nama
:
Umur
:
Alamat
:
Telah mendapat penjelasan secukupnya bahwa saya akan melakukan uji krim minyak alpukat terhadap kulit sebagai sediaan krim anti-aging. Setelah mendapat penjelasan secukupnya tentang manfaat penelitian ini dan efek sampingnya, maka saya menyatakan SETUJU untuk ikut serta dalam penelitian dari Cut Putri Arhandhi dengan judul “FORMULASI DAN UJI EFEK ANTIAGING DARI KRIM YANG MENGANDUNG MINYAK ALPUKAT (Avocado oil), sebagai usaha untuk mengetahui apakah sediaan krim anti-aging dari minyak alpukat yang dihasilkan mampu atau tidak dalam memulihkan kulit yang telah mengalami penuaan. Saya menyatakan sukarela dan bersedia untuk mengikuti prosedur penelitian yang telah ditetapkan. Persetujuan ini saya buat dengan penuh kesadaran dan tanpa paksaan dari pihak manapun. Demikian surat pernyataan ini dibuat untuk dapat dipergunakan sebagaimana mestinya. Medan, 27 November 2014 Peneliti,
Sukarelawan,
(Cut Putri Arhandhi)
(Nama Lengkap)
66 Universitas Sumatera Utara
Lampiran 11. Data statistik
1. Kadar air (moisture) ANOVA Sum of Squares Sebelum
df
Mean Square
Between Groups
2.500
5
.500
Within Groups
4.000
12
.333
Total
6.500
17
Between Groups
5.111
5
1.022
Within Groups
5.333
12
.444
Total
10.444
17
Between Groups
10.944
5
2.189
Within Groups
10.000
12
.833
Total
20.944
17
MingguIII Between Groups
15.611
5
3.122
6.000
12
.500
Total
21.611
17
MingguIV Between Groups
17.778
5
3.556
7.333
12
.611
25.111
17
MingguI
MingguII
Within Groups
Within Groups Total
F
Sig.
1.500
.261
2.300
.110
2.627
.079
6.244
.004
5.818
.006
67 Universitas Sumatera Utara
Lampiran 11. (Lanjutan) Sebelum Subset for alpha = 0.05 PEMULIHAN Tukey HSD
a
N
1
Formula A
3
27.67
Formula B
3
27.67
Formula C
3
28.33
Formula D
3
28.33
Formula E
3
28.33
Formula F
3
28.67
Sig.
.339
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
MingguI Subset for alpha = 0.05 PEMULIHAN Tukey HSD
a
N
1
Formula A
3
29.67
Formula B
3
30.00
Formula C
3
30.33
Formula D
3
30.67
Formula E
3
30.67
Formula F
3
31.33
Sig.
.082
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
68 Universitas Sumatera Utara
Lampiran 11. (Lanjutan) MingguII Subset for alpha = 0.05 PEMULIHAN Tukey HSD
a
N
1
2
Formula A
3
29.67
Formula B
3
31.00
31.00
Formula C
3
31.00
31.00
Formula D
3
31.00
31.00
Formula E
3
31.33
31.33
Formula F
3
32.33
Sig.
.506
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
MingguIII Subset for alpha = 0.05 PEMULIHAN Tukey HSD
a
N
1
2
3
Formula A
3
30.33
Formula B
3
30.67
30.67
Formula C
3
32.00
32.00
32.00
Formula D
3
32.00
32.00
32.00
Formula E
3
32.33
32.33
Formula F
3
33.00
Sig.
.538
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
69 Universitas Sumatera Utara
Lampiran 11. (Lanjutan) MingguIV Subset for alpha = 0.05 PEMULIHAN Tukey HSD
a
N
1
2
Formula A
3
30.33
Formula B
3
31.67
31.67
Formula C
3
32.33
32.33
Formula D
3
32.67
Formula E
3
33.00
Formula F
3
33.33
Sig.
.168
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
70 Universitas Sumatera Utara
Lampiran 11. (Lanjutan) 2. Kehalusan (evenness) ANOVA Sum of Squares Sebelum
Between
df
Mean Square
60.944
5
12.189
Within Groups
124.000
12
10.333
Total
184.944
17
44.278
5
8.856
92.667
12
7.722
136.944
17
32.278
5
6.456
78.000
12
6.500
110.278
17
67.778
5
13.556
90.000
12
7.500
Total
157.778
17
Between
155.611
5
31.122
82.000
12
6.833
237.611
17
F
Sig.
1.180
.375
1.147
.389
.993
.462
1.807
.186
4.554
.015
Groups
MingguI
Between Groups Within Groups Total
MingguII
Between Groups Within Groups Total
MingguIII
Between Groups Within Groups
MingguIV
Groups Within Groups Total
71 Universitas Sumatera Utara
Lampiran 11. (Lanjutan) Sebelum Subset for alpha = 0.05 PEMULIHAN Tukey HSD
a
N
1
Formula F
3
38.67
Formula E
3
39.33
Formula D
3
40.33
Formula C
3
41.33
Formula B
3
41.67
Formula A
3
44.33
Sig.
.322
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
MingguI Subset for alpha = 0.05 PEMULIHAN Tukey HSD
a
N
1
Formula F
3
37.33
Formula E
3
37.67
Formula D
3
38.67
Formula C
3
38.67
Formula B
3
40.00
Formula A
3
42.00
Sig.
.368
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
72 Universitas Sumatera Utara
Lampiran 11. (Lanjutan) MingguII Subset for alpha = 0.05 PEMULIHAN Tukey HSD
a
N
1
Formula F
3
35.00
Formula E
3
35.33
Formula D
3
36.00
Formula C
3
36.67
Formula B
3
38.00
Formula A
3
38.67
Sig.
.522
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
MingguIII Subset for alpha = 0.05 PEMULIHAN Tukey HSD
a
N
1
Formula F
3
32.33
Formula E
3
33.33
Formula D
3
34.00
Formula C
3
35.00
Formula B
3
36.67
Formula A
3
38.00
Sig.
.189
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
73 Universitas Sumatera Utara
Lampiran 11. (Lanjutan) MingguIV Subset for alpha = 0.05 PEMULIHAN Tukey HSD
a
N
1
2
Formula F
3
29.00
Formula E
3
29.33
Formula D
3
33.00
33.00
Formula C
3
33.33
33.33
Formula B
3
34.00
34.00
Formula A
3
37.67
Sig.
.310
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
74 Universitas Sumatera Utara
Lampiran 11. (Lanjutan) 3. Besar pori (pore) ANOVA Sum of Squares Sebelum
Between
df
Mean Square
21.778
5
4.356
Within Groups
168.000
12
14.000
Total
189.778
17
29.167
5
5.833
Within Groups
167.333
12
13.944
Total
196.500
17
Between
142.500
5
28.500
74.000
12
6.167
Total
216.500
17
Between
404.444
5
58.667
12
Total
463.111
17
Between
489.778
5
64.000
12
553.778
17
F
Sig.
.311
.897
.418
.827
4.622
.014
80.889 16.545
.000
Groups
MingguI
Between Groups
MingguII
Groups Within Groups
MingguIII
Groups Within Groups
MingguIV
4.889
97.956 18.367
.000
Groups Within Groups Total
5.333
75 Universitas Sumatera Utara
Lampiran 11. (Lanjutan) Sebelum Subset for alpha = 0.05 PEMULIHAN Tukey HSD
a
N
1
Formula F
3
24.33
Formula E
3
25.33
Formula D
3
25.67
Formula C
3
26.67
Formula B
3
27.33
Formula A
3
27.33
Sig.
.915
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
MingguI Subset for alpha = 0.05 PEMULIHAN Tukey HSD
a
N
1
Formula F
3
20.67
Formula E
3
22.33
Formula D
3
22.33
Formula C
3
23.00
Formula B
3
24.33
Formula A
3
24.33
Sig.
.828
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
76 Universitas Sumatera Utara
Lampiran 11. (Lanjutan) MingguII Subset for alpha = 0.05 PEMULIHAN Tukey HSD
a
N
1
2
Formula F
3
16.00
Formula E
3
18.67
18.67
Formula D
3
20.67
20.67
Formula C
3
22.00
22.00
Formula B
3
23.67
Formula A
3
24.00
Sig.
.163
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
MingguIII Subset for alpha = 0.05 PEMULIHAN Tukey HSD
a
N
1
2
3
Formula F
3
10.67
Formula E
3
14.00
Formula D
3
17.33
17.33
Formula C
3
17.33
17.33
Formula B
3
Formula A
3
4
14.00
23.33
23.33 24.00
Sig.
.999
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
77 Universitas Sumatera Utara
Lampiran 11. (Lanjutan) MingguIV Subset for alpha = 0.05 PEMULIHAN Tukey HSD
a
N
1
2
3
Formula F
3
8.00
Formula E
3
10.67
10.67
Formula D
3
14.00
14.00
14.00
Formula C
3
14.67
14.67
Formula B
3
Formula A
3
20.00
4
20.00 23.33
Sig.
.518
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
78 Universitas Sumatera Utara
Lampiran 11. (Lanjutan) 4. Noda (spot) ANOVA Sum of Squares Sebelum
Between
df
Mean Square
172.944
5
34.589
Within Groups
184.000
12
15.333
Total
356.944
17
17.333
5
3.467
Within Groups
266.667
12
22.222
Total
284.000
17
Between
189.778
5
37.956
Within Groups
150.667
12
12.556
Total
340.444
17
Between
437.111
5
87.422
88.000
12
7.333
Total
525.111
17
Between
860.500
5
172.100
84.000
12
7.000
944.500
17
F
Sig.
2.256
.115
.156
.974
3.023
.054
11.921
.000
24.586
.000
Groups
MingguI
Between Groups
MingguII
Groups
MingguIII
Groups Within Groups
MingguIV
Groups Within Groups Total
79 Universitas Sumatera Utara
Lampiran 11. (Lanjutan) Sebelum Subset for alpha = 0.05 PEMULIHAN Tukey HSD
a
N
1
Formula F
3
38.67
Formula E
3
41.67
Formula D
3
44.00
Formula C
3
45.00
Formula B
3
46.00
Formula A
3
48.33
Sig.
.087
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
MingguI Subset for alpha = 0.05 PEMULIHAN Tukey HSD
a
N
1
Formula F
3
38.33
Formula E
3
38.67
Formula D
3
38.67
Formula C
3
39.00
Formula B
3
40.33
Formula A
3
41.00
Sig.
.979
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
80 Universitas Sumatera Utara
Lampiran 11. (Lanjutan) MingguII Subset for alpha = 0.05 PEMULIHAN Tukey HSD
a
N
1
Formula F
3
29.67
Formula E
3
33.33
Formula D
3
35.67
Formula C
3
37.00
Formula B
3
38.67
Formula A
3
39.00
Sig.
.062
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
MingguIII Subset for alpha = 0.05 PEMULIHAN Tukey HSD
a
N
1
2
Formula F
3
24.67
Formula E
3
27.00
Formula D
3
32.00
Formula C
3
35.33
Formula B
3
36.00
Formula A
3
38.33
Sig.
32.00
.113
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
81 Universitas Sumatera Utara
Lampiran 11. (Lanjutan) MingguIV Subset for alpha = 0.05 PEMULIHAN Tukey HSD
a
N
1
2
3
4
Formula F
3
18.33
Formula E
3
22.33
Formula D
3
Formula C
3
Formula B
3
35.00
Formula A
3
38.00
Sig.
22.33 27.33
27.33 32.00
.472
.260
.322
32.00
.129
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
82 Universitas Sumatera Utara
Lampiran 11. (Lanjutan) 5. Keriput (wrinkle) ANOVA Sum of Squares Sebelum
Between
df
Mean Square
316.944
5
63.389
354.667
12
29.556
Total
671.611
17
Between
448.278
5
89.656
236.667
12
19.722
Total
684.944
17
Between
102.944
5
20.589
438.667
12
36.556
541.611
17
91.111
5
18.222
113.333
12
9.444
Total
204.444
17
Between
509.333
5
101.867
262.667
12
21.889
772.000
17
F
Sig.
2.145
.129
4.546
.015
.563
.727
1.929
.163
4.654
.014
Groups Within Groups
MingguI
Groups Within Groups
MingguII
Groups Within Groups Total MingguIII
Between Groups Within Groups
MingguIV
Groups Within Groups Total
83 Universitas Sumatera Utara
Lampiran 11. (Lanjutan) Sebelum Subset for alpha = 0.05 Pemulihan Tukey HSD
a
N
1
Formula F
3
27.67
Formula E
3
34.67
Formula D
3
35.33
Formula C
3
36.67
Formula B
3
39.00
Formula A
3
41.00
Sig.
.090
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
MingguI Subset for alpha = 0.05 Pemulihan Tukey HSD
a
N
1
2
Formula F
3
26.33
Formula E
3
27.67
27.67
Formula D
3
31.67
31.67
Formula C
3
34.67
34.67
Formula B
3
39.00
Formula A
3
39.00
Sig.
.074
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
84 Universitas Sumatera Utara
Lampiran 11. (Lanjutan) MingguII Subset for alpha = 0.05 Pemulihan Tukey HSD
a
N
1
Formula F
3
25.00
Formula E
3
27.67
Formula D
3
29.00
Formula C
3
30.67
Formula B
3
31.67
Formula A
3
31.67
Sig.
.754
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
MingguIII Subset for alpha = 0.05 Pemulihan Tukey HSD
a
N
1
Formula F
3
22.33
Formula E
3
22.67
Formula D
3
26.33
Formula C
3
26.33
Formula B
3
27.67
Formula A
3
28.00
Sig.
.281
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
85 Universitas Sumatera Utara
Lampiran 11. (Lanjutan) MingguIV Subset for alpha = 0.05 Pemulihan Tukey HSD
a
N
1
2
Formula F
3
14.00
Formula E
3
16.00
16.00
Formula D
3
22.33
22.33
Formula C
3
24.33
24.33
Formula B
3
27.67
Formula A
3
27.67
Sig.
.145
.083
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
86 Universitas Sumatera Utara
Lampiran 11. (Lanjutan) 6. Kedalaman keriput (wrinkle’s depth) ANOVA Sum of Squares Sebelum
Between
df
Mean Square
1080.944
5
184.000
12
1264.944
17
988.944
5
204.667
12
Total
1193.611
17
Between
1082.278
5
88.000
12
1170.278
17
866.278
5
198.667
12
Total
1064.944
17
Between
1636.444
5
342.667
12
1979.111
17
F
216.189 14.099
Sig. .000
Groups Within Groups Total MingguI
Between
15.333
197.789 11.597
.000
Groups Within Groups
MingguII
17.056
216.456 29.517
.000
Groups Within Groups Total MingguIII
Between
7.333
173.256 10.465
.000
Groups Within Groups
MingguIV
16.556
327.289 11.461
.000
Groups Within Groups Total
28.556
87 Universitas Sumatera Utara
Lampiran 11. (Lanjutan) Sebelum Subset for alpha = 0.05 PEMULIHAN Tukey HSD
a
N
1
2
Formula F
3
39.00
Formula E
3
42.33
Formula D
3
43.33
Formula C
3
47.00
Formula B
3
Formula A
3
3
47.00 56.00
56.00 60.67
Sig.
.694
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
MingguI Subset for alpha = 0.05 PEMULIHAN Tukey HSD
a
N
1
Formula F
3
35.67
Formula E
3
40.00
Formula D
3
42.33
Formula C
3
43.33
Formula B
3
Formula A
3
2
3
43.33 54.33
54.33 56.00
Sig.
.995
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
88 Universitas Sumatera Utara
Lampiran 11. (Lanjutan) MingguII Subset for alpha = 0.05 PEMULIHAN Tukey HSD
a
N
1
2
Formula F
3
32.00
Formula E
3
33.67
Formula D
3
38.00
Formula C
3
Formula B
3
Formula A
3
3
4
38.00 42.33
42.33 49.00
49.00 53.33
Sig.
.416
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
MingguIII Subset for alpha = 0.05 PEMULIHAN Tukey HSD
a
N
1
2
3
Formula F
3
28.00
Formula E
3
33.00
33.00
Formula D
3
33.33
33.33
Formula C
3
Formula B
3
45.33
Formula A
3
46.33
42.33
Sig.
42.33
.827
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
89 Universitas Sumatera Utara
Lampiran 11. (Lanjutan) MingguIV Subset for alpha = 0.05 PEMULIHAN Tukey HSD
a
N
1
2
3
Formula F
3
20.33
Formula E
3
24.00
Formula D
3
29.67
Formula C
3
42.00
42.00
Formula B
3
42.33
42.33
Formula A
3
29.67
44.33
Sig.
.993
Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 3,000.
90 Universitas Sumatera Utara
