DAFTAR PUSTAKA Ackermann HW, Dubow MS. 1987. Viruses of prokaryotes, vol II. Natural groups of bacteriophages. CRC Press, Inc., Boca Raton, Fla. Adisasmito W. 2007. Faktor Risiko Diare pada Bayi dan Balita di Indonesia: Systematic Review Penelitian Akademik Bidang Kesehatan Masyarakat. Makara, Kesehatan 11(1):1-10. Albert MJ, Faruque SM, Faruque AS, Neogi PK, Ansaruzzaman M, Bhuiyan NA, Alam K, Akbar MS. 1995. Controlled study of Escherichia coli diarrheal infections in Bangladesh children. J. Clin. Microbiol. 33:973-977. Albert MJ, Faruque AS, Faruque SM, Sack RB, Mahalanabis D. 1999. Casecontrol study of enteropathogens associated with childhood diarrhea in Dhaka, Bangladesh. J. Clin. Microbiol. 37:3458-3464. Alisky J, Iczkowski K, Rapoport A, Troitsky N. 1998. Bacteriophages show promise as antimicrobial agents. J. Infect. 36:5-15. Barrow PA, Soothill JS. 1997. Bacteriophage therapy and prophylaxis: rediscovery and renewed assessment of potential. Trends Genet. 5:268271. Bayer ME. 1968. Adsorption of Bacteriophages to Adhesions Between Wall and Membrane of Escherichia coli. J Virol 2(4):346-356. Bhan MK, Mahalanabis D, Fontaine O, Pierce NF. 1994. Clinical trials of improved oral rehydration salt formulations: a review. Bull. W.H.O. 72:945-955. Black RE, Merson MH, Rahman AS, Yunus M, Alim AR, Huq I, Yolken RH, Curlin GT. 1980. A two-year study of bacterial, viral, and parasitic agents associated with diarrhea in rural Bangladesh. J. Infect. Dis. 142:660-664. Bozzola JJ, Russell LD. 1998. Electron Microscopy Principles and Techniques for Biologist Second Edition. Jones and Bartlett Publishers:Massachusetts, USA. Bradford MM. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72:248-254. Budiarti S. 1997. Pelekatan pada sel HEp-2 dan keragaman serotipe O Escherichia coli enteropatogenik isolat Indonesia. Berkala Ilmu Kedokteran 29:105-110.
Budiarti S. 1998. Telaah faktor adhesivitas Escherichia coli enteropatogenik dalam penanggulangan penyakit diare di Indonesia. Laporan Penelitian Hibah Bersaing III/ Perguruan Tinggi DIKTI-Jakarta. Budiarti S, Mubarik NR. 2006. Extracellular Protease Activity of Enteropathogenic Escherichia coli on Mucin Substrate. Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University. Carlton RM, Noordman WH, Biswas B, de Meester ED, Loessner MJ. 2005. P100 for control of Listeria monocytogenes in foods: Genome sequence, bioinformatic analyses, oral toxicity study, and application. Regul Toxicol and Pharmacol 43:301-312. Casswall TH, Sarker SA, Faruque SM, Weintraub A, Albert MJ, Fuchs GJ, Alam NH, Dahlstrom AK, Link H, Brussow H, Hammarström L. 2000. Treatment of enterotoxigenic and enteropathogenic Escherichia coliinduced diarrhoea in children with bovine immunoglobulin milk concentrate from hyperimmunized cows: a double-blind, placebocontrolled, clinical trial. Scand. J. Gastroenterol. 35:711-718. Chibani-Chennoufi S, Sidoti J, Bruttin A, Dillmann M, Kutter E, Qadri F, Sarker SA, Brüssow H. 2004. Isolation of Escherichia coli Bacteriophages from the Stool of Pediatric Diarrhea Patients in Bangladesh. J Bacteriol 186(24):8287-8294. Clark JR, March JB. 2004. Bacterial viruses as human vaccines? Expert Rev. Vaccines 3:463-476. Defigueiredo MP, Splittstoesser DF. 1976. Food Microbiology: Public Health and Spoilage Aspects. The Avi Publishing Company, Inc: USA. Donnenberg MS, Whittam TS. 2001. Pathogenesis and evolution of virulence in Enteropathogenic and Enterohemorrhagic Escherichia coli.J. Clin. Investig.107(5). Ellis EL, Delbruck M. 1938. The Growth of Bacteriophage. Journal Gen Physiol:365-384. Farmer III JJ, Howard BJ, Weissfeld AS. 1987. Enterobacteriaceae, p317 in Howard BJ, Rubin SJ, Weissfeld AS, Tilton RC (eds) Clinical and pathogenic microbiology. The CV Mosby Co. St. Louis, Washington. Flynn GO, Ross RP, Fitzgerald GF, Coffey A. 2004. Evaluation of a cocktail of three bacteriophages for biocontrol of Escherichia coli O157:H7. Appl Environ Microbiol 70(6):3417-3424. Fortuna et al. 2008. Bacteriophage therapy in children: facts and prospects. J.Med
Sci Monit 14. Foschino R, Perrone F, Galli A. 1995. Characterization of two virulent Lactobacillus fermentum bacteriophages isolated from sour dough. J. Appl. Bacteriol. 79: 677-683. Girons IS, Margarita D, Amouriaux P, Baranton G. 1990. First Isolation Of Bacteriophages For A Spirochaete:Potential Genetic Tools For Leptospira. Res. Microbiol 141:1131-1138. Giron JA, Ho S, Schoolnik GK. 1991. An inducible bundle-forming pilus of enteropathogenic Escherichia coli. Science 258:710-713. Goode G, Allen VM, Barrow PA. 2003. Reduction of Experimental Salmonella and Campylobacter Contamination of Chicken Skin by Application of Lytic Bacteriophages. Appl Env Microbiol: 5032-5036. Goodridge L, Gallaccio A, Griffiths MW. 2003. Morphological, Host Range, and Genetic Characterization of Two Coliphages. Appl Environ Microbiol 69(9):5364-5371. Greenwood D, Slack RCB, Peutherer JF. 1995. Medical Microbiology. Churchill Livingstone, Medical Division of Pearson Professional Ltd: Hongkong. Gurnev PA, Oppenheim AB, Winterhalter M, Bezrukov SM. 2006. Docking of a single phage lambda to its membrane receptor maltoporin as a timeresolved event. J Mol Biol 359(5):1447-1455. Hagens S, Offerhaus ML. 2008. Bacteriophages, New Weapons for Food Safety. Food Technol 62(4):46-54. Hicks S, Frankel G, Kaper JB, Dougan G, Phillips AD. 1998. Role of Intimin and Bundle-Forming Pili in Enteropathogenic Escherichia coli Adhesion to Pediatric Intestinal Tissue In Vitro. Infect Immun. 66: 1570-1578. Hoque SS, Faruque AS, Mahalanabis D, Hasnat A. 1994. Infectious agents causing acute watery diarrhoea in infants and young children inBangladesh and their public health implications. J. Trop. Pediatr. 40:351-354. Hughes KA, Sutherland IA, Jones MV. 1998. Biofilm susceptibility to bacteriophage attack: the role of phage-borne polysaccharide depolymerase. Microbiology 144:3039-3047. Inglis TJJ. 1996. Microbiology and Infections. A clinically orientated core text with self-assessment. Churchill Livingstone:New York. International Committee on Taxonomy of Viruses. 2000. Virus taxonomy; classification and nomenclature of viruses. Sixth report of the International
Committee on Taxonomy of Viruses. Springer-Verlag, Vienna, Austria:49-54. Irianto J. 2000. http://digilib.litbang.depkes.go.id/go.php?id=jkpkbppk-gdl-res2000-joko-1085-diare. Jarvis KG, Giron JA, Jerse AE, McDaniel TK, Donnenberg MS, Kaper JB. 1995. Enteropathogenic Escherichia coli contains a putative type III secretion system necessary for the export of proteins involved in attaching and effacing lesion formation. Proc Natl Acad Sci. USA 92:7996-8000. Jay JM. 1978. Modern Food Microbiology, second edition. Van Nostrand Reinhold Company:New York. Jay JM, Loessner MJ, Golden DA. 2005. Modern Food Microbiology, seventh edition. Springer Science, Business Media, CLC: USA. Kenny BR, DeVinney R, Stein M, Reinsheid DJ, Frey EA, Finlay BB. 1997. Enteropathogenic Escherichia coli (EPEC) transfer its receptor for intimate adhesion into mammalian cell. Cell 91:511-529. Keogh BP, Pettingill G. 1966. Long-Term Storage of Bacteriophages of Lactic Streptococci. Appl Environ Microbiol. 14(3): 421-424. Knutton S, Lloyd DR, McNeish AS. 1987. Adhesion of enteropathogenic Escherichia coli to human intestinal enterocytes and cultured human intestinal mucosa. Infect. Immun. 55:69-77. Kudva IT, Jelacic S, Tarr PI, Youderian P, Hovde CJ. 1999. Biocontrol of Escherichia coli O157 with O157-Specific Bacteriophages. Appl Environ Microbiol 65(9):3767-3773. Laemmli EK. 1970. Cleavage of structural protein during the assembly of head of bacteriophage T4. Nature 227:680-685. Lay BW, Hastowo S. 1992. Mikrobiologi. Jakarta:Rajawali Pers. Levine MM, Nataro JP, Karch H, Baldini MM, Kaper JB, Black RE, Clements ML, O’Brien AD. 1985. The diarrheal response of humans to some classic serotypes of enteropathogenic Escherichia coli is dependent on a plasmid encoding an enteroadhesiveness factor. Infect. Dis. 152(3):550-559. Levine MM. 1987. Escherichia coli that cause diarrhea: enterotoxigenic enteropathogenic, enteroinvasive, enterohaemorrhagic and enteroadherent. Infect Dis 155:377-389. Ma YL, Lu CP. 2008. Isolation and identification of a bacteriophage capable of infecting Streptococcus suis type 2 strains. Vet Microbiol xxx
Nataro JP, Kaper JB. 1998. Diarrheagenic Escherichia coli. Clin Microbiol 11:142-201. Ochman H, Selander RK. 1984. Standard reference strains of Escherichia coli from natural populations. J Bacteriol 157:690-693. Ogunseitan OA, Sayler GS, Miller RV. 1992. Application of DNA probes to analysis of bacteriophage distribution patterns in the environment. Appl Environ Microbiol 58:2046-2052. Park SC, Nakai T. 2003. Bacteriophage control of Pseudomonas plecoglossicida infection in ayu Plecoglossus altivelis. Dis Aquat Organ 53:33-39. Park SC, Shimamura I, Fukunaga M, Mori KI, Nakai T. 2000. Isolation of bacteriophages specific to a fish pathogen, Pseudomonas plecoglossicida, as a candidate for disease control. Appl Environ Microbiol 66:1416-1422. Pawsey RK. 2002. Case Studies in Food Microbiology for Food Safety and Quality. Royal Society of Chemistry: London, United Kingdom. Pelczar MJ, Chan ESC. 1988. Dasar-Dasar Mikrobiologi. Diterjemahkan oleh R.S. Hadioetomo, T. Imas, S.S. Tjitrosomo dan S.L. Angka. Universitas Indonesia:Jakarta. Pierson MD, Stern NJ. 1986. Foodborne Microorganisms and Their Toxins: Developing Methodology. Marcel Dekker, Inc: New York, USA. Pitt TL, Gaston MA. 1995. Bacteriophage Typing. Methods in Molecular Biology 46:15-26. Projan S. 2004. Phage-inspired antibiotics?. Nat Biotechnol 22:167-168. Randerson J. 2003. Virus cleans up food poisoning bug. New Sci:2392. Salyers AA, Whitt DD. 1994. Bacterial Pathogenesis, A Molecular Approach. Departement of Microbiology, University of Illionis. ASM Press:Washington DC. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular Cloning: a Laboratory Manual. Second edition. Cold Spring Harbor Laboratory Pr, Cold Spring Harbor:New York. Savarino SJ, Hall ER, Bassily S, Wierzba TF, Youssef FG, Peruski Jr. LF, AbuElyazeed R, Rao M, Francis WM, El Mohamady H, Safwat M, Naficy AB, Svennerholm AM, Jertborn M, Lee YJ, Clemens JD. 2002. Introductory evaluation of an oral, killed whole cell enterotoxigenic Escherichia coli plus cholera toxin B subunit vaccine in Egyptian infants. Pediatr. Infect. Dis. J. 21:322-330.
Shuren J. 2006 in Federal Register, ed US Food and Drug Administration (Natl Arch and Records Admin, Washington, DC) 71:47729-47732. Skurnik M. 1984. Lack of correlation between the presence of plasmids and fimbriae in Yersinia enterocolitica and Yersinia pseudotuberculosis. J Appl Bacteriol 56:355-363. Skurnik M, Strauch E. 2006. Phage therapy: Facts and fiction (Review). International Journal of Medical Microbiology 296:5-11. Smith HW, Huggins MB, Shaw KM. 1987. The control of experimental Escherichia coli diarrhea in calves by means of bacteriophages. J. Gen. Microbiol. 133:1111-1126. Snyder JD, Merson MH. 1982. The magnitude of the global problem of acute diarrhoeal disease: a review of active surveillance data. Bull. W.H.O. 60:605-613. Strauch E, Kaspar H, Schaudinn C, Dersch P, Madela K, Gewinner C, Hertwig S, Wecke J, Appel B. 2001a. Characterization of enterocoliticin, a phage taillike bacteriocin, and its effect on pathogenic Yersinia enterocolitica strains. Appl Environ Microbiol 67:5634-5642. Topley WWC, Wilson GS. 1990. Principles of bacteriology, virology and immunity. B.C. Decker Publisher: London, United Kingdom. Tortora GJ, Funke BR, Case CL. 2006. Microbiology: an Introduction (ninth edition). Benjamin Cummings:New York. Travis J. 2008. Curious Cat Science and Engineering Blog © curiouscat.com. WordPress. Warouw S. 2002. http://digilib.litbang.depkes.go.id/go.php?id=jkpkbppk-gdlres-2002-sonny-836-lingkungan. Waturangi DE. 1999. Purifikasi dan Karakterisasi Protease Ekstraseluler Enteropatogenik Escherichia coli [Tesis]. Program Pascasarjana, Institut Pertanian Bogor:Bogor. Wendelschafer-crabb G, Erlandsen SL, Walker DH, JR. 1975. Conditions Critical for Optimal Visualization of Bacteriophage Adsorbed to Bacterial Surfaces by Scanning Electron Microscopy. J Virol 15(6):1498-1503. Yoon, Sung-Sik, Barrangou-Poueys R, JR FB, Fleming HP. 2007. Detection and Characterization of a Lytic Pediococcus Bacteriophage from the Fermenting Cucumber Brine. J Microbiol Biotechnol 17(2):262-270.
LAMPIRAN
Lampiran 1 Pembuatan pereaksi Bradford untuk pengukuran konsentrasi protein Coomassie Brilliant Blue G-250 sebanyak 100 mg dilarutkan di dalam 50 ml etanol 95%. Kemudian ditambahkan 100 ml asam orto-fosfat 85% dan diencerkan dengan akuades sampai satu liter. Larutan ini kemudian disaring dengan kertas saring. Larutan stok lima kali ini dapat disimpan beberapa bulan pada suhu 4 0C dan apabila akan digunakan, satu bagian larutan stok ini diencerkan dengan empat bagian akuades.
Lampiran 2 Komposisi bahan untuk membuat gel pengumpul dan gel pemisah Konsentrasi No. 1.
Komponen
Gel Pengumpul
Gel Pemisah
(4%)
(10%)
0.67 ml
3.3 ml
-
2.5 ml
Larutan Stok Akrilamida 30%
2.
Buffer Gel Pemisah
3.
Buffer Gel Pengumpul
1.25 ml
-
4.
Akuades
3.075 ml
4.1 ml
5.
SDS 10% (w/v)
0.05 ml
0.1 ml
6.
Amonium persulfat 10% (w/v)
0.05 ml
0.1 ml
7.
TEMED
0.005 ml
0.01 ml
Jumlah
5.1 ml
10.11 ml
Lampiran 3 Komposisi larutan stok SDS-PAGE (Laemmli 1970) 1. Larutan stok akrilamida 30% w/v Akrilamida sebanyak 29.2 gram dilarutkan dalam 80 ml air bebas ion, kemudian ditambahkan 0.8 gram bis-akrilamida. Setelah larut, volumenya ditepatkan menjadi 100 ml. Larutan ini kemudian disaring dengan kertas saring dan disimpan dalam botol gelap pada suhu 4 0C. Hindari kontak langsung dengan kulit atau masuk ke dalam tubuh, sebab akrilamida dan bis akrilamida bersifat neurotoksik. 2. Buffer gel pemisah (1.4 M Tris-Cl pH 8.8) Sebanyak 45.5 gram Tris base dilarutkan dalam 200 ml air bebas ion dan pH-nya disesuaikan menjadi 8.8 dengan HCl. Kemudian 1 gram SDS ditambahkan dan volumenya ditepatkan menjadi 250 ml. 3. Buffer gel pengumpul (1.4 M Tris-Cl pH 6.8) Sebanyak 15.1 gram Tris base dilarutkan dalam 200 ml air bebas ion dan pH-nya disesuaikan menjadi 6.8 dengan HCl. Kemudian 1 gram SDS ditambahkan dan volumenya ditepatkan menjadi 250 ml. 4. Sodium Dodesil Sulfat (SDS) 10% Sebanyak 10 gram SDS dilarutkan ke dalam 100 ml air bebas ion kemudian disaring. Larutan ini disimpan pada suhu ruang. 5. Amonium persulfat (APS) 10% Sebanyak 1 gram amonium persulfat dilarutkan ke dalam 10 ml air bebas ion. Larutan ini sebaiknya selalu dibuat segar. 6. Stok double strength buffer sampel Sebanyak 0.15 gram Tris base dilarutkan dalam 5 ml air bebas ion, lalu dicampur dengan 2 ml gliserol, 1 ml merkaptoetanol dan 1 ml bromofenol blue (0.1% w/v bromofenol blue dalam air bebas ion). Kemudian pH-nya disesuaikan menjadi 6.8 dengan menambahkan HCl dan ditambahkan SDS 0.46 gram. Volumenya kemudian ditepatkan menjadi 10 ml.
Lampiran 4 Prosedur pewarnaan perak (silver stain) 1.
Pewarnaan perak dilakukan menggunakan sarung tangan dan peralatan yang benar-benar bersih, karena kebersihan alat akan mempengaruhi sensitifitas reaksi.
2.
Gel elektroforesis, direndam dalam 5 kali volume gel larutan 30% ethanol dan 10% asam asetat selama 3 jam hingga satu malam pada suhu ruang sambil diagitasi perlahan.
3.
Larutan ethanol dan asam asetat kemudian dibuang, dan gel direndam dalam 5 kali volume larutan 30% ethanol. Inkubasi gel ini selama 30 menit pada suhu ruang sambil diagitasi perlahan. Ulangi perendaman ini sekali lagi.
4.
Buang larutan ethanol, lalu cuci gel dalam 10 kali volume gel air bebas ion dan diinkubasi selama 10 menit. Ulangi pencucian ini dua kali.
5.
Gel kemudian direndam dalam 5 kali volume gel larutan 0.1% AgNO3 yang diencerkan dari stok 20% (w/v). Inkubasi gel ini selama 30 menit, pada suhu ruang, kemudian gel dicuci dengan air bebas ion yang dialirkan selama 20 detik.
6.
Tambahkan 5 kali volume gel larutan 2.5% NaCO3 dan 0.02% formaldehid dan gel diinkubasi pada suhu ruang sambil diagitasi hingga terbentuk pitapita dalam beberapa menit. Apabila background mulai berwarna, hentikan inkubasi dengan mencuci gel dalam larutan 1% asam asetat.
7.
Kemudian cuci gel dengan air bebas ion selama 10 menit. Ulangi pencucian ini beberapa kali.
Lampiran 5 Kurva standar protein (Bovine Serum Albumin)
0.300
Absorban
0.250 0.200 0.150 y = 0.0024x + 0.0145
0.100
R2 = 0.9749
0.050 0.000 0
20
40
60
80
mg/L (ppm)
Konsentrasi BSA (mg/L)
Absorban
Blanko 1 2 3 4 5 10 15 20 40 60 80 100
0.158 0.161 0.165 0.175 0.181 0.186 0.194 0.220 0.226 0.290 0.335 0.376 0.390
100
120
