UJI KANDUNGAN FLAVONOID PADA EKSTRAK DAUN SALAM (Eugenia polyantha Wight)
Hesti Devinta G1G012031
BAB I PENDAHULUAN A. Latar Belakang Tumbuhan obat di Indonesia melimpah
Ekstrak
Senyawa aktif
Flavonoid
Daun Salam (Eugenia polyantha Wight)
B. Tujuan Penelitian Mengetahui pembuatan ekstrak etanol daun salam serta kandungan senyawa aktif flavonoid pada ekstrak daun salam (Eugenia polyantha Wight) C. Manfaat Penelitian Menambah wawasan penulis dan pembaca serta menambah referensi institusi mengenai kandungan senyawa aktif flavonoid pada ekstrak daun salam (Eugenia polyantha Wight)
BAB II TINJAUAN PUSTAKA A. Landasan Teori 1. Daun Salam (Eugenia polyantha Wight) a. Taksonomi b. Morfologi c. Kandungan senyawa aktif 2. Ekstraksi a. Gambaran Umum b. Metode 1) Cara Dingin: Maserasi dan perkolasi 2) Cara Panas: Refluks, sokletasi, digesti, infus dan dekok 3. Flavonoid
BAB III METODE PENELITIAN A. Kerangka Konsep Variabel bebas: Ekstrak daun salam (Eugenia polyantha Wight)
Variabel tak terkendali: Paparan zat lain Variabel terikat: Senyawa aktif Flavonoid Variabel terkendali: 1. Alat yang digunakan 2. Pelarut etanol 96 % 3. Suhu ruangan
B. Hipotesis Terdapat senyawa aktif berupa flavonoid pada ekstrak daun salam (Eugenia polyantha Wight) C. Jenis dan Rancangan Penelitian Jenis penelitian ini adalah penelitian eksperimental laboratoris. D. Lokasi dan Waktu Penelitian Laboratorium taksonomi tumbuhan fakultas biologi dan laboraturium biologi farmasi UNSOED pada bulan November.
E. Variabel Penelitian 1. Variabel Bebas: Daun Salam 2. Variabel Terikat: Senyawa aktif flavonoid 3. Variabel Terkendali: Alat yang digunakan, pelarut etanol 96%, suhu ruangan F. Definisi Operasional 1. ekstrak daun salam (rasio) 2. flavonoid (Nominal) G. Sumber Data
H. Instrumen 1. Alat: Oven, timbangan, evaporator, bekker glass, waterbath 2. Bahan: Daun salam, etanol 96 %, HCL pekat, serbuk Mg I. Cara Pengumpulan Data 1. Determinasi tanaman daun salam 2. Pembuatan ekstrak daun salam 3. Uji Fitokimia Flavonoid
Pembuatan ekstrak daun salam a. Daun salam dibersihkan lalu diiris menjadi potonganpotongan halus danmenghasilkan 350 gram b. Ekstraksi dilakukan dengan cara maserasi dengan menggunakan etanol 96%, sampel dimaserasi dalam bekker glass selama 5 hari dan sesekali diaduk c. Filtrasi sebanyak 2x d. Evaporasi menggunakan evaporator pada suhu 60°c sampai menjadi endapn yang tidak terlalu kental (Sekitar 90 menit) e. Pengeringan dengan menggunakan waterbath.
Uji Fitokimia Flavonoid a. Ekstrak kental etanol daun salam ditimbang sebanyak 1 g dan dipanaskan b. Lapisan atas dipipet dan ditambahkan dengan 1 tetes HCL pekat 2N serta 0,1 serbuk Mg c. Hasil positiv ditunjukan dengan munculnya warna merah yang mengindikasikan adanya senyawa aktif flavonoid
Membangun Jejaring 1. Lokasi Penelitian a. Gedung farmasi FIKES Universitas Jenderal Soedirman
b. Gedung Biologi Universitas Jenderal Soedirman
2. Meminta kesediaan untuk menjadi dosen pembimbing lapangan 3. Mengurus perizinan praktik lapangan 4. Memberikan surat kepada kepala laboratorium
PEMBIMBING DOSEN LAPANGAN
Harwoko, M. Sc. Apt
Curriculum Vitae Personal Data Nama : Harwoko, M. Sc. Apt TTL : Pekalongan, 5 Juli 1984 Alamat : Perum. Puri Karanggitung C-6 Sumbang, Banyumas, Jawa Tengah Pendidikan : 2002-2006 S.Farm, UGM 2006-2007 Apt., UGM 2011-2013 M. Sc, UGM Kegiatan Mengajar: Phytochemistry Pharmacognosy Phytotherapy Research Methodology Pharmareutical Botany
RESEARCH EXPERIENCES 2015 (On going)
-Bioassay-guided fractionation of latropa curcas leaf to isolate bioactive compound as antiinflammation (Fundamental research-DIKTI) -- Effect of purified extract of Psidium guajava leaf on cutaneus anaphylactic reaction (Compotitive grantDIKTI) -- Effect of flavonoid-rich fraction of Centella asiatica leaf on type 2 diabetes mellitus and insulin resistance rats (Institutional research-UNSOED)
2014
-Potency of bioactive compounds of Brotowali as chemotherapeutic on colon cancer cell with cellular approach -Development of Brotowali stem purified extract as antihyperuricemic (in vivo and in vitro study)
2012
Bioactivity study of Myrmeleon sp. As hypoglychemic drugs
2011
Bioactivity study of losartan on aminotransferase serum level and liver fibrosis degree in rats
2010-2011
Antipoliferative effects of protein fraction from Jatropha curcas leaf in mice skin exposed to DMBA and UVB
2010
Hepatoprotective effect of waru leaves infusion in oaracetamol induced hepatotoxic rats
CONFERENCES AND SCIENTIFIC MEETINGS 2015
-The 4th International Conference on Pharmacy and Advanced Pharmaceutical Sciences -The 5th International Conference on Plant Diversity -The International Conference on Herbal Medicine Industrialization as Complementary Theraphy in Natural Disasters
2014
International Symposium on Medicinal Plant and Traditional Medicine
2012
International Conference on Medicinal Plants: “Scientification of Jamu (Evidence Based Jamu Development): A breakthrough Program from Plant to Medicine for Health Care
2011
-The 1st International Conference in Medicine and Health Sciences -The 2nd International Conference on Pharmacy and Advanced Pharmaceutical Sciences
TRAINING, COURSE, AND WORKSHOP 2015
Course on Quality Control of Material and Herbal Product (Batch 1)
2014
Course on International Publication (Batch II)
2012
Workshop on Manuscript Writing for Journal Publication
2011
Workshop on Quality Assurances of Biopharmaca Material and Product
2010
The 18th Advanced course in Pharceutical Science: Workshop on Development of Phytochemicals into Commercial Pharmaceutical Poducts”
2010
Course on National Scientific Article Writing
ORGANIZATION AND OTHER EXPERIENCES 2015present
Head, Laboratory of Pharmaceutical Biology, Universitas Jenderal Soedirman
2015
Workshop on Pharmacy movement for 50 student creativity programs (PKM)
2014
Seminar on Improving UNSOED lecturer’s competence through International Publications Purwokerto Indonesia, June 5th (adviser)
2014
Patient Conseling Workshop on the right usage of herbal medication counseling, “Using traditional medicine appropriately adn safely”. Purwokerto Indonesia. September 29th, 2011
2014present
Member of organizational and leadership Indonesian Pharmacist Association (IAI)
2010-2011
Coach of student activity unit of Pharmacy drug information service (UKM PIO)
2010-2015
Trainer on Development Program of Student Character and Personality (PKKM UNSOED)
2011-2012
Guest lecturer of Academid of Health Science “Al- Irsyad
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