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Lampiran 1. Kapal tuna longline
Spesifikasi Kapal : Panjang total Panjang garis tegak Lebar Tinggi Sarat air sesuai desain (Desaigned draft) Berat muatan Isi kotor Isi bersih Fish bait hold Bait hold Fuel oil tank Fresh water tank Mesin Kecepatan
: 23.40 m : 18.45 m : 5.20 m : 2.20 m : 1.60 m : 60 ton : 86 ton : 51 ton : 32 m3 : 2.3 m3 : 18,7 m3 : 4.5 m3 : YANMAR 240 PS X 2000 RPM : 9 knot
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Lampiran 2. Pengukuran panjang ikan tuna mata besar di kapal tuna longline
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Lampiran 3. Kriteria tingkat kematangan gonad menurut Schaefer dan Orange ( 1956) Tingkat Keadaan gonad (ovary) I. Dara Berkembang (Immature)
Deskripsi
Gonad memanjang dan ramping, jenis kelamin dapat ditentukan dengan kaca pembesar. Ovari jernih berwarna abu-abu hingga kemerah-merahan, telur satu per satu dapat dilihat dengan kaca pembesar
II. Perkembangan I (early maturing)
Ovary membcsar, berwarna kemerah-merahan dengan pembuluh kapiler,. bulatan telur belum dapat terlihat dengan mata telanjang, ovari mengisi sekitar setengah ruang bawah
III. Perkembangan II (late maturing)
Ovary membesar dan membengkak , berwarna orange kemerah-merahan, butiran telur sudah dapat terlihat dengan mata biasa, ovari mengisi 2/3 ruang bawah
IV. Matang (Ripe)
Ovari sangat membesar, butiran telur membesar dan berwarna jernih, dapat keluar dari lumen dengan sedikit penekanan pada bagian perut, gonad mengisi penuh ruang bawah.
V. Memijah (Spawning)
Termasuk yang memijah sekarang (salin) dan mijah sebelumnya (post-spawning) , ovari sangat besar dan lunak (karena mijah). Telur matang yang tertinggal dalam keadaan terserap, telur berwarna jernih dan ada yang masih tertinggal dalam ovari. Telur akan keluar dengan sedikit penekanan pada perut.
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Lampiran 4. Teknik Pembuatan Preparat Histologi (Angka et al., 1996) 1. Fiksasi : sampel/jaringan dimasukkan ke dalam larutan fiksatif (Bouins alkohol) yaitu campuran asam fikrat, formalin, dan asam asetat pekat dengan perbandingan 15:5:1. Larutan ini berfungsi untuk mencegah terjadinya kerusakan jaringan, terjadinya proses osmosis, penciutan jaringan dan mengawetkan jaringan agar mendekati atau sama seperti asalnya. 2. Dehidrasi : sampel dipindahkan secara bertahap ke dalam alkohol 80%, 90%, 95% dan 100% masing-masing selama 2 jam. Selanjutnya sampel dipindahkan kedalam alkohol 100% selama semalam. 3. Clearing : sampel dipindahkan kedalam alkohol 100% baru selama satu jam. Selanjutnya dipindahkan kedalam alkohol-xylol, xylol I, xylol II dan xylol III, masing-masing selama setengah jam. 4. Impregnasi : Sampel dipindahkan kedalam xylol : parafin (1:1) selama ¾ jam (didalam oven) pada suhu 65-70oC 5. Embedding : sampel dipindahkan ke dalam parafin I, parafin II, dan parafin III masing-masing selama ¾ jam. 6. Blocking : sampel dikeluarkan dari parafin, kemudian dicetak dalam cetakan dan didiamkan selama semalam. 7. Pemotongan jaringan. Sampel dipotong setebal 5-6 μm. Selanjutnya potongan sampel ditetesi larutan albumin gliserin agar sampel jaringan teregang. Gelas benda (object glass) dengan jaringan di ataasnya ditaruh di atas hot plate suhu 40oC agar agak kering. Langkah berikutnya sampel diwarnai. 8. Pewarnaan jaringan : Setelah disayat maka dilakukan proses dehidrasi. Gelas benda berisi jaringan dimasukkan ke dalam xylol I, xylol II. Alkohol 100%I, 100% II, 95%, 90%, 80%, 70% dan 50% masing-masing selama 3 menit, kemudian preparat dicuci sampai berwarna putih. Selanjutnya diwarnai dengan haematocylin selama 5-7 menit, dicuci dengan air 5-7 menit, direndam dalam larutan eoisin 3 menit dan dicuci dengan air kran mengalir. Setelah dicuci kembali dilakukan dehidrasi, caranya yaitu memasukkan gelas benda yang berisi jaringan ke dalam alkohol 50%, 70%,85%, 90%, 95%, 100% I,
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100% II, xylol I dan xylol II masing-masing selama 2 menit. Langkah berikutnya tetesi dengan balsem Canada atau Entellen dan langsung ditutup dengan gelas penutup. Sampel
dibiarkan selama semalam (12 jam) agar
kering dan tidak ada udara antara gelas tutup dan gelas benda. Selanjutnya sampel diamati di bawah mikroskop.
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Lampiran 5. Sebaran Frekuensi Ukuran Kelas Panjang dan Hubungan Panjang Berat Ikan Tuna Mata Besar (T.obesus) a. Sebaran Frekuensi Ukuran kelas Panjang Kelas ukuran panjang (cm) 108-115 116-123 124-131 132-139 140-147 148-155 Jumlah
Jumlah ikan (ekor) 6 6 8 13 8 1 42
b. Hubungan Panjang Berat Model Regresi Ikan Tuna Mata Besar (Thunnus obesus) Berat Total = 0,0381 + 2, 8623 Panjang Cagak Koefisien : a = 0,0381 b = 2,8623 Regression Statistics Multiple R 0,9875 R Square 0,9753 Adjusted R Square 0,9500 Standard Error 20,8477 Observations 42
c. Uji –t
Mean Variance Observations Pearson Correlation df t Stat t table
Variable 1 130,40 115,32 42 0,96 41 26,77 2,02
Variable 2 44023,81 113096980,3 42
Hipotesis Ho : b = 3 (pola pertumbuhan isometric) Hipotesis H1 : b ≠ 3 (pola pertumbuhan allometrik) Hasil perhitungan nilat t hitung > t tabel , maka keputusannya tolak Ho
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Lampiran 6. Faktor kondisi Ikan Tuna Mata Besar (T.obesus) a. Hubungan Faktor kondisi dengan Ukuran Panjang Kelas Panjang (cm) 108-115 116-123 124-131 132-139 140-147 148-155
Faktor kondisi 2,016 1,922 2,003 1,915 1,921 2,038
Berat rata-rata (gr) 28833,33 33333,33 42625,00 47230,77 56000 73000
b. Hubungan Faktor Kondisi dengan TKG TKG I II IV
Faktor kondisi 1,942 1,981 1,897
Berat rata-rata (g) 43071,43 45166,67 50500
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Lampiran 7. Tingkat Kematangan Gonad (TKG) dan Indeks Kematangan Gonad (IKG) Ikan Tuna Mata Besar (T.obesus) a. TKG pada Selang Kelas Panjang Kelas ukuran panjang (cm) I 4 4 8 6 5 1 28
108-115 116-123 124-131 132-139 140-147 148-155 Jumlah
Jumlah II 2 2 0 6 2 0 12
b. Hubungan TKG dengan IKG TKG I II IV
Rata-rata IKG 0,496 0,888 2,093
c. Hubungan TKG dengan Waktu Bulan Maret April Mei Oktober Jumlah
I 2 4 22 0 28
Jumlah II 6 6 0 0 12
d. Hubungan IKG dengan Waktu Bulan Maret April Mei Oktober
IKG rata-rata 0,897 0,656 0,491 2,093
IV 0 0 0 2 2
IV 0 0 0 1 1 0 2
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Lampiran 8. Pola Sebaran Diameter Telur Ikan Tuna Mata Besar (T.obesus) a. Pola Sebaran Diameter Telur Ikan Tuna Mata Besar (T.obesus) Gabungan. Selang kelas diameter telur (μm) 99,49-165,94 165,95-232,30 232,31-298,76 298,77-365,22 365,23-431,68 431,69-498,14 498,15-564,60 564,61-631,06 631,07-697,52 697,53-763,98 763,99-830,44 Jumlah
Frekuensi (%) 0,33 2,83 16,00 8,67 21,83 27,83 5,50 8,67 4,67 2,17 1,50 600
b. Pola Sebaran Diameter Telur Ikan Tuna Mata Besar (T.obesus) Bagian Posterior
30 20 10 0 99 ,5 -1 73 ,4 0 17 3, 41 -2 47 ,3 1 24 7, 32 -3 21 ,2 2 32 1, 23 -3 95 ,1 3 39 5, 14 -4 69 ,0 4 46 9, 05 -5 42 ,9 5 54 2, 96 -6 16 ,8 6 61 6, 87 -6 90 ,7 7 69 0, 78 -7 64 ,6 8 76 4, 69 -8 38 ,5 9
Jumlah telur (butir)
n=200 60 50 40
Selang Kelas Diameter Telur (µm)
Jumlah Telur (butir) 99 ,5 -1 73 ,4 0 17 3, 41 -2 47 ,3 1 24 7, 32 -3 21 ,2 2 32 1, 23 -3 95 ,1 3 39 5, 14 -4 69 ,0 4 46 9, 05 -5 42 ,9 5 54 2, 96 -6 16 ,8 6 61 6, 87 -6 90 ,7 7 69 0, 78 -7 64 ,6 8 76 4, 69 -8 38 ,5 9
Jumlah telur (butir) 40 35 30 25 20 15 10 5 0
60 50 40 30 20 10 0
17 3, 41 -2 47 ,3 1 24 7, 32 -3 21 ,2 2 32 1, 23 -3 95 ,1 3 39 5, 14 -4 69 ,0 4 46 9, 05 -5 42 ,9 5 54 2, 96 -6 16 ,8 6 61 6, 87 -6 90 ,7 7 69 0, 78 -7 64 ,6 8 76 4, 69 -8 38 ,5 9
99 ,5 -1 73 ,4 0
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c. Pola Sebaran Diameter Telur Ikan Tuna Mata Besar (T.obesus) Bagian Median
n=200
Selang Kelas Diameter Telur (µm)
d. Pola Sebaran Diameter Telur Ikan Tuna Mata Besar (T.obesus) Bagian Anterior
n=200
Selang Kelas Diameter Telur (µm)
